CN113509413A - Plant extract composite bacteriostatic agent and application thereof - Google Patents
Plant extract composite bacteriostatic agent and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of cosmetics, and particularly relates to a plant extract composite bacteriostatic agent and application thereof, wherein the plant extract composite bacteriostatic agent comprises the following raw materials in percentage by mass: 1-30% of bacteriostatic agent, 0.5-50% of synergistic agent and 20-98.5% of solvent; the plant extract composite bacteriostatic agent has good bacteriostatic, itching relieving and anti-allergy effects, good safety and no toxic or side effect on a human body, and can be widely applied to various skin care cosmetics as a bacteriostatic and anti-allergy active ingredient.
Description
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to a plant extract composite bacteriostatic agent and application thereof.
Background
The preservative is an indispensable component in the cosmetics, and the preservative can be added to inhibit the breeding of microorganisms in the cosmetics and protect the products from being polluted by the microorganisms in the production or use process, so that the selection of the efficient and safe preservative is one of the keys in the formula development of the cosmetics. According to technical Specification for safety of cosmetics (2015 edition), there are 51 kinds of preservatives for cosmetic preparations at present, including p-hydroxybenzoate esters, phenoxyethanol, isothiazolinone and the like.
In recent years, along with the development of society and the improvement of living standard, people have made higher demands for the diversification of cosmetic functions and product quality. As consumers are deeply fixed on the concept of nature and health, the cosmetic has strong psychological repulsion on chemical additives, and the concepts of 'zero addition' and 'no corrosion' are vigorously developed in the cosmetic industry. Therefore, in order to meet the increasing demand of consumers, the development of cosmetics without adding chemical preservatives has been a hot spot.
Disclosure of Invention
In order to overcome the defects in the prior art, the first object of the present invention is to provide a plant extract composite bacteriostatic agent, wherein the components of the plant extract composite bacteriostatic agent interact with each other and are optimally combined with a synergist to finally provide a plant extract composite bacteriostatic agent capable of long-acting bacteriostasis, oxidation resistance and sensitization improvement, and the components of the plant extract composite bacteriostatic agent have bacteriostasis synergy effects.
The invention also aims to provide application of the plant extract composite bacteriostatic agent in preparation of cosmetics.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme:
the plant extract composite bacteriostatic agent is characterized by comprising the following raw materials in percentage by mass: 1-30% of bacteriostatic agent, 0.5-50% of synergistic agent and 20-98.5% of solvent, wherein the bacteriostatic agent is one or a mixture of cinnamon bark extract and cortex moutan radicis bark extract.
Preferably, the plant extract composite bacteriostatic agent consists of the following raw materials in percentage by mass: 5-20% of bacteriostatic agent, 2-20% of synergistic agent and 60-93% of solvent, wherein the bacteriostatic agent is one or a mixture of cinnamon bark extract and cortex moutan radicis bark extract.
More preferably, the plant extract composite bacteriostatic agent consists of the following raw materials in percentage by mass: 19% of cinnamon bark extract, 16% of synergistic agent and 65% of solvent.
More preferably, the plant extract composite bacteriostatic agent consists of the following raw materials in percentage by mass: 19% of cortex moutan radicis extract, 16% of synergistic agent and 65% of solvent.
More preferably, the plant extract composite bacteriostatic agent consists of the following raw materials in percentage by mass: 11% of cinnamon bark extract, 8% of cortex moutan radicis extract, 16% of synergistic agent and 65% of solvent.
The synergistic agent is caprylyl hydroximic acid, ethylhexyl glycerol, p-hydroxyacetophenone, levulinic acid, sodium levulinate, glyceryl monocaprylate, glyceryl undecylenate, glyceryl monolaurate, dipropylene glycol, 1, 2-octanediol, azelaic acid, magnolia bark extract, sophora flavescens extract, artemisia leaf extract, benzoin extract, star anise extract, brazil tree bark extract, hyacinth bean extract, cardamom extract, oldenlandia diffusa extract, willow bark extract, birch bark extract, lalang grass rhizome extract, lilac extract, atractylodes macrocephala extract, pulsatilla chinensis extract, thyme extract, barbat skullcap extract, mint extract, Cedar bark extract, Schisandra chinensis extract, Chinese arborvitae leaf extract, artemisia scoparia extract, saffron extract, xanthium sibiricum extract, atractylodes lancea extract, thyme extract, scutellaria baicalensis extract, mint extract, schisandra chinensis extract, hinokra extract, artemisia extract, saffron extract, xanthium extract, atractylodes rhizome extract, and/or other, Katsumadai seed extract, tsaoko amomum fruit extract, sweet clover extract, Chinese arborvitae twig extract, camellia extract, tea extract, bupleurum extract, calamus extract, ivy extract, pulsatilla koreana extract, angelica koreana extract, plantain extract, agilawood extract, daisy extract, agilawood extract, codonopsis pilosula extract, phellodendron amurense extract, pericarpium zanthoxyli extract, elecampane extract, ligusticum wallichii extract, andrographis paniculata extract, acanthopanax senticosus extract, plantago asiatica extract, soybean seed extract, galangal, rhodiola crenulata, purslane, opuntia ficus, thistle extract, barley extract, sargentgloryvine stem extract, plantago asiatica extract, jujube extract, salvia miltiorrhiza extract, hawthorn fruit extract, lophatherum gracile/stem extract, angelica extract, codonopsis pilosula extract, camellia extract, tea extract, bupleurum chinense extract, ligusticum wallichii extract, plantain extract, plantain seed extract, etc Sword bean extract, rehmannia root extract, garden burnet root extract, juniper chinensis leaf/stem extract, polygonatum kingianum extract, clove leaf extract, angelica acutangula extract, white gourd extract, pubescent angelica root extract, eucommia bark extract, schizosaccharomyces fermentation product extract, fermentation monospore fungus fermentation product extract, french wintersweet extract, french rose extract, french lavender extract, papaya extract, tomato fruit/leaf/stem extract, senna leaf extract, ledebouriella root extract, soapwort extract, tetrandra root extract, passionflower extract, propolis extract, honey extract, royal jelly extract, phoenix-tail extract, impatiens balsamina flower/leaf/stem extract, fingered citron extract, poria cocos extract, chlorella pumila extract, raspberry extract, licorice extract, honey extract, and honey extract, Chamomile extract, nardostachys chinensis extract, galangal extract, alpine edelweiss extract, kudzu root extract, medlar extract, hypericum perforatum extract, patchouli extract, desmodium styracifolium extract, dioscorea panthaica extract, curcuma kwangsiensis extract, osmanthus fragrans extract, peronospora bungeana extract, hypsizygus marmoreus extract, tropaeolum majus extract, myrobalan extract, albizia julibrissin flower extract, albizia julibrissin bark extract, polygonum multiflorum extract, black sesame extract, black tea extract, red clover extract, safflower extract, rhodiola rosea extract, red yeast rice extract, picrorhiza sativa extract, pepper extract, mistletoe extract, polygonum cuspidatum extract, interbedded-leaf and leaf/stem extract, zanthoxylum bungeanum fruit extract, sophora flower extract, phellodendron amurense bark extract, artemisia leaf extract, airpotato extract, goldenrod extract, yellow flower extract, kombum extract, lycium chinensis extract, lycium chinense extract, hypericum japonicum extract, echinacea extract, hypericum extract, hyperi, Coptis chinensis extract, scutellaria baicalensis extract, anise fruit extract, fennel extract, glechoma longituba extract, suberect spatholobus stem extract, centella asiatica extract, pseudoalteromonas sp fermentation product extract, ash bark extract, dandelion extract, sisal extract, ginger extract, turmeric extract, gynostemma pentaphyllum extract, yeast lysate extract, yeast extract, elderberry extract, lobelia extract, dendrobium nobile extract, chamomile extract, longhairy antenoron herb extract, vitex negundo leaf/root/stem extract, schizonepeta herb extract, sarcandra glabra extract, murraya paniculata leaf extract, platycodon grandiflorum extract, artemisia chrysanthemums extract, chicory extract, kava root extract, sophora flavescens root extract, momordica charantia fruit extract, quassia wood extract, aloe vera extract, mentha flower/leaf/stem extract, holly leaf/stem extract, platycodon grandiflorum extract, artemisia grandiflorum extract, chrysanthemum extract, chicory extract, kava extract, kava sylvestris extract, horseradish root extract, capsicum extract, forsythia extract, lotus flower extract, lotus leaf extract, lotus seed extract, campsis grandiflora extract, spearmint flower/leaf/stem extract, reed rhizome extract, aloe extract, arundo donax stem extract, pilose antler extract, Thujopsis dolabrata branch extract, momordica grosvenori fruit extract, podocarpus macrophylla extract, ocimum basilicium flower/leaf/stem extract, mung bean extract, purslane extract, calla flower/stem extract, ophiopogon root extract, vanilla extract, myrrh extract, acacia melissa officinalis extract, rosemary extract, changium smyrnioides extract, jasmine extract, clove extract, chamomile extract, peony extract, oroxylum indicum extract, hibiscus bark extract, akebia stem extract, costustoot extract, artemisia scoparia extract, kadsura longepedunculata extract, kadsura japonica extract, kadsura longa flower extract, and a kadsura longepedwood extract, a flower extract, a kadsura longflower extract, a flower extract, a kadsura longflower extract, a joa flower extract, a kadsura longflower extract, a flower extract, a faga flower extract, a, Lemon extract, burdock root extract, oregano extract, glossy privet fruit extract, thyme extract, salvia extract, syringa vulgaris extract, rubia cordifolia root extract, inula japonica extract, agrimony extract, aesculus hippocastanum extract, berberis thunbergii rhizome/root extract, loquat fruit/leaf extract, hop extract, brewer's yeast extract, grape seed extract, grapefruit seed extract, dandelion extract, horse chestnut extract, madder extract, rose extract, zanthoxylum fruit extract, artemisia apiacea extract, ginseng root extract, honeysuckle extract, hinoki leaf extract, cnidium officinale root extract, angelica japonica extract, coptis japonica root extract, coptis japonica extract, cistanche deserticola extract, nutmeg extract, lactic acid bacteria fermentation extract, lactic acid bacteria fermentation extract, lactic acid bacteria, lactic, Olibanum extract, Notoginseng radix extract, caulis Akebiae stem extract, Mori fructus extract, folium Mori extract, herba Taxilli extract, flos Camelliae Japonicae extract, rhizoma Dioscoreae extract, Corni fructus extract, radix Paeoniae extract, fructus Cnidii extract, herba Thymi vulgaris extract, folium Ocimi Pilosi extract, rhizoma Acori Graminei extract, flos Granati extract, semen Granati extract, Caryophyllum chinensis extract, cornflower extract, herba Salvia officinalis extract, herba Menthae extract, herba Silybi Mariani extract, lignum sappan extract, semen Ziziphi Spinosae extract, radix Pseudostellariae extract, fructus Rhodomyrti extract, Terna folium et cacumen extract, Lonicera Japonica flos extract, rhizoma Gastrodiae extract, radix asparagi extract, herba Speranskiae Tuberculatae extract, rhizoma Smilacis Glabracteati extract, flos Tagetis Erectae extract, semen Vaccariae extract, flos Gomphrenae/flower extract, flos Magnoliae extract, radix Linderae extract, herba Taxilli chinensis extract, herba Taxilli extract, herba Taraxaci extract, radix Morindae officinalis extract, radix Morindae officinalis extract, radix Morindae officinalis extract, and radix Morindae officinalis extract, radix extract, and radix Morindae officinalis extract, and radix extract, Fig extract, soapberry extract, evodia fruit extract, Chinese gall extract, cortex acanthopanacis extract, schisandra fruit extract, American ginseng extract, sambucus williamsii flower extract, cinnamomum zeylanicum bark extract, leonurus tenuifolius extract, senna angustifolia leaf extract, selfheal extract, opuntia ficus chinensis extract, cactus extract, orange peel extract, citronella extract, bergamot fruit extract, bergamot leaf extract, fructus forsythiae extract, wheat extract, apricot extract, bearberry extract, spiraea crispa extract, usnea paniculata extract, figwort extract, inula japonica extract, saussurea involucrata extract, nettle extract, mushroom extract, bacillus/soybean fermentation product extract, bacillus/rice bran extract/soybean extract fermentation product filtrate, and the like, Oat extract, sage leaf extract, rhubarb extract, polygonatum rhizome/root extract, white sage extract, soybean extract, crataegus fruit extract, motherwort extract, starwort extract, ginkgo nut extract, ginkgo leaf extract, epimedium extract, neem extract, commiphora mukul resin extract, cherry leaf extract, chestnut seed extract, camellia oleifera leaf extract, camellia oleifera seed extract, pomelo peel extract, pomelo seed extract, houttuynia cordata extract, spiraea ulmaria flower extract, corn poppy flower extract, cinnamon extract, polygonatum odoratum extract, guaiacum extract, coriander fruit/leaf extract, peppermint leaf extract, angelica sinensis, extract, polygala tenuifolia extract, evening primrose extract, vietnamese benzoin resin extract, costus root extract, yuba root extract, white sage extract, yunnan benzoin resin extract, etc, One or more of a Yunnan coptis extract, a date fruit extract, a gleditsia sinensis extract, a spina gleditsiae extract, a quillaja bark extract, an alisma orientale extract, a camphor leaf extract, an amygdalus pedunculata extract, a selfheal extract, a jonquil extract, a rheum palmatum extract, a horse chestnut extract, a pearl extract, a sesame extract, a rhizoma anemarrhenae extract, a gardenia extract, an upright stemona root extract, a rehmannia glutinosa root extract, a polyporus umbellatus extract, a panax japonicus extract, a zanthoxylum bungeanum extract, a lithospermum extract, a mirabilis jalapa extract, a perilla extract, a pterocarpus indicus extract, a wisteria extract, a ganoderma extract and a palm extract.
Preferably, the synergistic agent is one or a mixture of more than one of schisandra chinensis extract, levulinic acid and saccharomyces bifidus fermentation product extract.
The solvent is one or a mixture of more than one of 1, 2-propylene glycol, 1, 3-propylene glycol, 1, 2-butylene glycol, 1, 3-butylene glycol, 1, 4-butylene glycol, 1, 2-pentanediol, 1, 5-pentanediol, isoprene glycol, 1, 2-hexanediol, 1, 6-hexanediol, ethanol, menthol, laurocapram, water, glycerol and methyl propylene glycol.
The plant extract composite bacteriostatic agent is prepared by the following steps: and (3) stirring and dissolving the bacteriostatic agent, the synergistic agent and the solvent at room temperature, and uniformly mixing to obtain the plant extract composite bacteriostatic agent.
The application of the plant extract composite bacteriostatic agent in preparing the cosmetics is characterized in that the cosmetics contain the plant extract composite bacteriostatic agent, and the cosmetics are selected from any one of facial mask liquid, emulsion, cream, toner, essence, stock solution, facial cleanser, toner, perfume, makeup remover, foundation solution, foundation cream, concealer, blusher, lipstick, eye shadow and blusher.
The application of the plant extract composite bacteriostatic agent in preparing cosmetics is characterized in that the dosage of the plant extract composite bacteriostatic agent is 0.5-5% by mass percent.
Compared with the prior art, the invention has the beneficial effects that:
1. the components of the plant extract composite bacteriostatic agent are common plant extracts, and the active ingredients are mutually coordinated and promoted, so that the bacteriostatic agent has good bacteriostatic, antioxidant and anti-allergy effects, can replace the traditional preservative, and has the advantages of safety, mildness and no stimulation.
2. According to the invention, the plant extracts such as the schisandra chinensis extract, the levulinic acid and the saccharomyces cerevisiae fermentation product extract are added into the plant extracts such as the cinnamon bark and the cortex moutan root bark, so that the curative effect of the plant extracts can be effectively improved in the preparation, storage and use processes of cosmetics; meanwhile, the synergists have a synergistic effect, and can obviously improve the antibacterial performance of the cinnamon bark extract and the antibacterial and anti-allergy performance of the cortex moutan radicis bark extract.
Detailed Description
The invention is further illustrated by the following examples. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention. Experimental procedures without specific conditions noted in the examples below, generally according to conditions conventional in the art or as suggested by the manufacturer; the raw materials, reagents and the like used are, unless otherwise specified, those commercially available from the conventional markets and the like. Any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.
Example 1
The extraction process of the cinnamon bark comprises the following steps:
taking 55kg of cinnamon bark, crushing by using a traditional Chinese medicine crusher, and sieving by using a 50-mesh sieve to obtain cinnamon bark powder. Taking 50kg of sieved cortex Cinnamomi powder, adding 5-15 times of distilled water, soaking for 18-24h, putting into a steam distillation device, rapidly heating at high power, reducing heating power after steam is generated, and continuously extracting for 4 h. Extracting the distillate with 50L diethyl ether twice, mixing the two extracted diethyl ether layers, adding small amount of anhydrous sodium sulfate, drying, heating for 20min, and steaming to remove diethyl ether to obtain oily extract of cortex Cinnamomi.
Example 2
The extraction process of root bark of the peony tree comprises the following steps:
50kg of cortex moutan radicis is taken, crushed by a traditional Chinese medicine crusher and sieved by a sieve of 80 meshes for standby. Adding the fine powder of cortex moutan into Soxhlet extractor, extracting with n-hexane as solvent at 70-80 deg.C under reflux for 24 hr. After extraction, the extract is decompressed and concentrated by a rotary evaporator to obtain the oily extract of the cortex moutan which is preserved in dark.
Preparation of plant-derived composite bacteriostatic agents of examples 3-13 and comparative examples 1-6 in each ratio:
according to the mass percentage composition of the table 1, the bacteriostat, the synergistic agent and the solvent are uniformly stirred and mixed and dissolved, and the plant extract composite bacteriostat with each proportion can be obtained.
Table 1. plant extract composite bacteriostatic agent in each proportion.
Test example 1. minimum inhibitory concentration test.
1. Experimental reagent: nutrient broth (Guangdong Huaqiao microbiological science, Inc., 022010); sterilizing fructus Hordei Germinatus extract decoction (027130, Kyoto cyclochei microorganism technology Co., Ltd.) in deionized water; 0.85% physiological saline, sterilizing and using.
2. Experimental materials: the bacteriostatic agents are shown in the table 1.
3. The species and source of the experimental strain: escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC9027, Staphylococcus aureus ATCC6526, Pseudomonas putida GDMCC NO.60411, Aspergillus niger ATCCL6404 and Candida albicans ATCCL0231 which are all from Guangdong province microorganism strain preservation center; burkholderia cepacia CGMCC No.5384, which is from the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms.
4. And (4) preparing a bacterial suspension.
TABLE 2 proportion of McLeod turbiditubes
The turbidimetric vials prepared according to the proportions in table 2 were used as standard bacterial suspension controls. Respectively adding sterile 0.85% of normal saline (0.85% of NaCl) into each strain slant, respectively washing out a certain amount of bacteria, then adding each washed-out bacteria liquid into a sterile test tube with the same diameter (size) as that of the McLeod's turbidimetric standard tube, respectively adding sterile 0.85% of normal saline (0.85% of NaCl) for dilution until the concentration is the same as that of the McLeod's turbidimetric standard tube, taking the diluted bacteria liquid as a bacteria suspension, and finally diluting each bacteria suspension by 100 times for later use.
5. And (4) determining the minimum inhibitory concentration.
Taking 12 sterile test tubes, sequentially marking, transferring 3000 μ L of liquid culture medium (nutrient broth for bacteria, and wort extract for fungi) into the sterile test tubes with pipette gun, and sequentially adding 3000 μ L of prepared liquid culture medium C into the 12 test tubes1，C2，C3，C4… … sample (C)1，C2，C3，C4… … concentrations were set according to each example test range and gradient decreasing), 3 replicates per concentration; in addition, with 1 control sample, 3000. mu.L of liquid medium and 3000. mu.L of 0.85% physiological saline were added, and finally, 300. mu.L of each bacterial suspension was added to the test tube; plugging the inoculated test tube with a plug, and placing the test tube inoculated with the microorganism in a shaking table at 30 ℃ for culturing for 48 hours; taking out the culture medium at 24h and 48h, shaking the test tube, and if sediment is produced at the bottom or the solution is turbid, the strain cannot be inhibited from growing at the concentration; on the other hand, the concentration is such that the growth of the species is inhibited (for example, in E.coli inoculated tubes, the concentration is C1，C2，C3The test tube of (A) produced no precipitate and the solution was relatively clear at a concentration of C4The bottom of the test tube is precipitated or the solution is turbid,the minimum concentration of this sample to inhibit E.coli is C3)。
TABLE 3 statistical results of minimum inhibitory concentration (unit: ppm)
As shown in table 3, it can be seen from the comparison of examples 3, 6 and 9 with comparative example 1 that the schisandra chinensis extract, levulinic acid and saccharomyces cerevisiae fermentation product extract all enhance the bacteriostatic performance of the cinnamon bark extract; comparing examples 4, 7 and 10 with comparative examples 2, 3, 4 and 5, it can be seen that the cortex moutan radicis and cortex moutan extract has slightly poor antibacterial performance, but the compound of the cortex moutan radicis and the synergist shows synergistic antibacterial effect; in addition, by respectively comparing examples 3-5, 6-8 and 9-11, the cortex moutan radicis extract and the cinnamon extract can be synergistic, so that the antibacterial effect is improved; in addition, in comparative examples 5, 8, 11, 12 and 13, it can be found that the schisandra chinensis extract, levulinic acid and the schizosaccharomyces cerevisiae fermentation product extract have a synergistic interaction effect, so that the antibacterial effect of the composition of the cinnamon bark extract and the cortex moutan radicis bark extract can be further improved.
Test example 2 Induction of inhibition of histamine Release amount from macrophage tumor cells in P815 mice.
Histamine is often used as one of the early diagnostic indicators of acute allergic reactions. The histamine is directly involved in hypersensitivity, the content of the histamine is related to the occurrence and the severity of the hypersensitivity, and the antiallergic capability of the sample can be evaluated by detecting the content of the histamine.
1. Experimental materials: DMEM medium, FBS, MTT, dansyl chloride, NaHCO3Ammonia, acetonitrile, ammonium acetate.
2. Preparing a sample to be tested: the samples of examples 3-13 and comparative examples 1-6 were diluted to a concentration of 10% with DMEM medium, respectively, for use.
3. Experiment ofThe method comprises the following steps: taking P815 cells (mouse mastocytoma cells, GDC099) in logarithmic growth phase, and diluting the cells to 6 × 10 concentration by DMEM medium4one/mL. The diluted cell solutions were inoculated into 96-well plates with an inoculum size of 1mL per well at 37 ℃ with 5% CO2The culture box is used for culturing for 24 hours. Then dividing the test sample into a blank control group, a model control group and a sample group, wherein each group is provided with 6 complex holes, 50 mu L of corresponding samples to be tested are respectively added into the test sample group, 50 mu L of DMEM culture medium is respectively added into the blank control group and the model control group, the test sample group and the model control group are placed in an incubator to be pre-incubated, and after 1h, except the blank control group, cells of each group are placed under a 15W ultraviolet lamp and irradiated for 30s to induce P815 cells to generate anaphylactic reaction and release histamine. Then put 5% CO2The culture box is used for culturing for 24 hours. After 24h, 200. mu.L of the culture medium was removed for derivatization of histamine, and saturated NaHCO was added in sequence3The solution (300. mu.L) and dansyl chloride (300. mu.L) at 20mg/mL were reacted at room temperature for 1 hour, then 20% ammonia-acetonitrile solution (100. mu.L) was added to terminate the reaction, and after 1 hour, 100. mu.L of acetonitrile solution was added, and HPLC analysis was performed after filtration through a membrane.
4. Result judgment
Preparing a solution with the concentration of 1,5, 10, 20 and 50ppm by using a histamine standard sample, filtering the solution by using a filter membrane, performing HPLC analysis, drawing a standard curve by using the concentration of the histamine standard sample as a horizontal coordinate and using a corresponding chromatographic peak area as a vertical coordinate, and calculating the concentration value of each sample according to the standard curve.
Table 4. test results of the inhibition effect experiment of the plant extract composite bacteriostatic agent on histamine release amount.
As shown in table 4, it can be seen from comparative examples 3 to 11 and comparative examples 1 to 6 that the cortex moutan radicis extract and the schisandra chinensis extract have a significant inhibitory effect on the amount of histamine released; the addition of the schisandra chinensis extract and the schizosaccharomyces cerevisiae fermentation product extract can effectively enhance the inhibition effect of the cortex moutan radicis extract on the histamine release amount; in addition, the results of comparative examples 5, 8, 11, 12 and 13 show that the Schisandra chinensis extract and the Zygosaccharomyces bifidus fermentation product extract have synergistic effect.
Test example 3 human body patch test.
1. The purpose of the test is as follows: the safety of examples 3 to 13 was examined.
2. Preparing facial mask liquid: examples 3-13 were each added to a facial mask solution prepared according to the formulation of table 5 below.
TABLE 5 facial mask liquid formulation
Preparation method of facial mask liquid
Adding 1, 3-propylene glycol, and adding xanthan gum, sclerotium rolfsii gum and hydroxyethyl cellulose into a stirring pot while stirring until the mixture is uniformly stirred and dispersed;
continuously stirring the allantoin, the trehalose, the amino acid humectant, the sodium hyaluronate and the EG-1 until the allantoin, the trehalose, the amino acid humectant, the sodium hyaluronate and the EG-1 are uniformly stirred and dispersed;
adding 70 parts of deionized water, and then heating to the system temperature of about 85-90 ℃;
standing, keeping the temperature for 20min for sterilization, slowly cooling to below 60 deg.C under stirring, adding sufficient deionized water, and adding collagen powder, vitamin B3, and K2 into the system;
continuously stirring and cooling to about 40 ℃, adding the composition into the system, continuously stirring for about 10min, stopping stirring, cooling, and then inspecting to discharge materials;
and sealing the storage barrel after discharging, and moving to a standing room for later use.
3. The test method comprises the following steps: a48 h closed patch test was used. Respectively weighing 0.02mL of diluted aqueous solution of different mask liquid products into a spot tester, and simultaneously setting a blank control group, wherein the control group is the diluent of the cosmetic. 50 healthy volunteers are selected, the age is 18-24 years, and no allergic history exists. After cleaning the inner forearm of the subject, 0.02mL of sample was applied to a piece of filter paper attached to the plaque tester. The patch tester is applied to the inner side of forearm of the subject with non-irritating adhesive tape, and then is applied to skin by pressing with fingers for 48 h. The examinee is ordered to keep the spot-pasting part dry within 48h, and violent exercise, scratching the spot-pasting part, long-time sunlight irradiation and the like are avoided. After 48h, the tester is removed and marked, after 30min, the pressure marks disappear under sufficient light for judgment, and the revisit observation is carried out within 72h and 96h respectively.
TABLE 6 grading Standard of adverse skin reactions
TABLE 7 Patch test results
Claims (10)
1. The plant extract composite bacteriostatic agent is characterized by comprising the following raw materials in percentage by mass: 1-30% of bacteriostatic agent, 0.5-50% of synergistic agent and 20-98.5% of solvent, wherein the bacteriostatic agent is one or a mixture of cinnamon bark extract and cortex moutan radicis bark extract.
2. The plant extract composite bacteriostatic agent according to claim 1, which is prepared from the following raw materials in percentage by mass: 5-20% of bacteriostatic agent, 2-20% of synergistic agent and 60-93% of solvent, wherein the bacteriostatic agent is one or a mixture of cinnamon bark extract and cortex moutan radicis bark extract.
3. The plant extract composite bacteriostatic agent according to claim 2, which is prepared from the following raw materials in percentage by mass: 19% of cinnamon bark extract, 16% of synergistic agent and 65% of solvent.
4. The plant extract composite bacteriostatic agent according to claim 2, which is prepared from the following raw materials in percentage by mass: 19% of cortex moutan radicis extract, 16% of synergistic agent and 65% of solvent.
5. The plant extract composite bacteriostatic agent according to claim 2, which is prepared from the following raw materials in percentage by mass: 11% of cinnamon bark extract, 8% of cortex moutan radicis extract, 16% of synergistic agent and 65% of solvent.
6. The plant extract composite bacteriostatic agent according to claim 1, wherein the synergistic agent is caprylyl hydroximic acid, ethylhexyl glycerin, p-hydroxyacetophenone, levulinic acid, sodium levulinate, glyceryl monocaprylate, glyceryl undecylenate, glyceryl monolaurate, dipropylene glycol, 1, 2-octanediol, azelaic acid, magnolia bark extract, sophora flavescens extract, artemisia leaf extract, benzoin extract, star anise extract, brazil tree bark extract, white hyacinth bean extract, cardamom extract, oldenlandia diffusa extract, willow bark extract, birch bark extract, lalang grass rhizome extract, lilac alba extract, atractylodes macrocephala koidz extract, pulsatilla chinensis extract, thyme extract, sculellaria barbata extract, mint extract, Cedar asiatica bark extract, Schisandra chinensis extract, Hinoki leaf extract, artemisia scoparia extract, saffron extract, xanthium sibiricum extract, atractylodes rhizome extract, alpinia katsumadai extract, tsaoko amomum fruit extract, sweet clover extract, biota orientalis leaf extract, camellia extract, tea leaf extract, bupleurum root extract, calamus extract, ivy extract, pulsatilla koreana extract, angelica koreanum extract, plantain herb extract, agilawood extract, daisy extract, agilawood extract, codonopsis pilosula extract, phellodendron amurense extract, pericarpium zanthoxyli extract, elecampane extract, ligusticum wallichii extract, andrographis paniculata extract, acanthopanax senticosus extract, plantago asiatica extract, soybean seed extract, galangal, rhodiola crenulata, purslane extract, opuntia ficus majus, setose thistle extract, barley extract, sargentgloryvine stem extract, plantain herb extract, jujube extract, salvia miltiorrhiza extract, sedum bungeanum kadsura segetum extract, sedum bungeanum extract, sedum maxim extract, sedum extract, alpine extract, sedum bunge rose extract, alpine extract, kadsia japonica extract, kadsura japonica, Fructus crataegi Pinnatifidae extract, lophatherum gracile/stem extract, angelica sinensis extract, codonopsis pilosula extract, sword bean extract, rehmannia glutinosa extract, sanguisorba officinalis extract, juniper chinensis leaf/stem extract, polygonatum kingianum extract, clove leaf extract, angelica sinensis extract, white gourd extract, pubescent angelica root extract, eucommia ulmoides extract, schizosaccharomyces pombe fermentation product extract, zymomonas campestris fermentation product extract, french wintersweet extract, french rose extract, french lavender extract, papaya extract, tomato fruit/leaf/stem extract, senna leaf extract, saposhnicovia divaricata extract, saposhnikovia officinalis extract, tetrandra powder extract, passionflower flower extract, propolis extract, honey extract, royal jelly extract, phoenix-tail fern extract, impatiens balsamina flower/leaf/stem extract, fingered citron extract, codonopsis pilosula extract, sword bean extract, rehmannia glutinosa extract, sanguisorba officinalis extract, polygonum japonicum extract, and honey extract, Poria cocos extract, poria cocos wolf extract, chlorella pumila extract, raspberry extract, licorice extract, chamomile extract, nardostachys chinensis extract, galangal rhizome extract, alpine edelweiss extract, pueraria root extract, lycium barbarum extract, hypericum perforatum extract, patchouli extract, desmodium styracifolium extract, dioscorea panthaica extract, curcuma kwangsiensis extract, osmanthus fragrans extract, peronospora bungeana extract, hypsizygus marmoreus extract, tropaeolum majus extract, myrobalan extract, albizia flower extract, albizia bark extract, polygonum multiflorum extract, black sesame extract, black tea extract, red clover extract, safflower extract, rhodiola rosea extract, red yeast rice extract, picrorhiza rhizome extract, pepper extract, mistletoe extract, polygonum cuspidatum extract, alternanthera flower/leaf/stem extract, zanthoxylum bungeanum fruit extract, zanthoxylum bungeanum extract, polygonum japonicum extract, polygonum bungeanum extract, polygonum bunge extract, polygonum multiflorum extract, polygonum bunge extract, kam, polygonum bunge extract, Sophora flower extract, phellodendron bark extract, artemisia annua leaf extract, vitex negundo extract, coptis chinensis extract, scutellaria baicalensis extract, anise fruit extract, fennel extract, red sage root extract, suberect spatholobus stem extract, centella asiatica extract, pseudoalteromonas fermentation product extract, ash bark extract, dandelion, sisal, ginger, turmeric, gynostemma pentaphyllum, yeast lysate, yeast extract, elderberry extract, Chinese lobelia extract, dendrobium stem extract, chamomile extract, lysimachia christinae extract, vitex bean leaf/root/stem extract, schizonepeta herb extract, sarcandra glabra extract, murraya jasminorage leaf extract, platycodon grandiflorum extract, artemisia chrysanthemi extract, chicory extract, kava root extract, kava root extract, kava sylvestris extract, Picrashiza root extract, momordica charantia fruit extract, quassia extract, aloe vera extract, peppermint/leaf/stem extract, horseradish root extract, capsicum extract, forsythia suspensa extract, lotus flower extract, lotus leaf extract, lotus seed extract, campsis grandiflora extract, spearmint flower/leaf/stem extract, reed rhizome extract, aloe extract, arundo donax stem extract, deer antler extract, thujoram twig extract, momordica grosvenori fruit extract, podocarpus macrophyllus extract, basil flower/leaf/stem extract, mung bean extract, purslane extract, calla flower/stem extract, ophiopogon root extract, vanilla extract, myrrh extract, acacia meili fruit extract, rosemary extract, changium smyrnioides extract, jasmine extract, clove extract, chamomile extract, peony extract, aloe vera extract, lily flower/leaf/stem extract, aloe vera extract, momordica flower extract, momordica grosvenori fruit extract, momordica flower extract, ginseng extract, jasmine extract, champigweed extract, aloe vera flower extract, aloe extract, lily extract, etc, Oroxylum indicum extract, hibiscus bark extract, akebia stem extract, costus root extract, artemisia scoparia extract, kadsura longepedunculata extract, lemon extract, burdock root extract, oregano extract, glossy privet fruit extract, thyme extract, salviae miltiorrhizae extract, syringa vulgaris extract, rubia cordifolia root extract, inula japonica extract, agrimony extract, aesculus hippocastanum extract, berberis thunbergii rhizome/root extract, loquat fruit/leaf extract, hops extract, brewer's yeast extract, grape seed extract, grapefruit seed extract, dandelion extract, horse chestnut extract, madder extract, rose extract, zanthoxylum piperitum fruit extract, artemisia apiacea extract, ginseng root extract, honeysuckle flower extract, japanese cypress leaf extract, japanese ligusticum wallichii root extract, japanese angelica extract, japanese mugwort extract, kadsura japonica extract, japanese mugwort extract, japanese cinnamon extract, and the like extract, Japanese coptis root extract, cistanche extract, myristica fragrans extract, lactic acid bacteria fermentation extract, frankincense extract, pseudo-ginseng extract, akebia trifoliata stem extract, mulberry fruit extract, mulberry leaf extract, parasitic loranthus extract, camellia extract, yam extract, dogwood fruit extract, peony extract, cnidium fruit extract, thyme extract, holy basil leaf extract, grassleaf sweelflag rhizome extract, pomegranate flower extract, pomegranate seed extract, carnation extract, cornflower extract, sage extract, peppermint extract, silybum marianum extract, sappan wood extract, wild jujube seed extract, pseudostellaria root extract, myrtle fruit extract, tonna leaf extract, Japanese honeysuckle extract, gastrodia tuber extract, asparagus extract, speranskia herb extract, glabrous greenbrier rhizome extract, marigold flower extract, lactobacillus fermentation extract, frankincense extract, pseudo-ginseng extract, trifoliate akebia stem extract, mulberry fruit extract, mulberry leaf extract, mulberry fruit extract, parasitic loranthus extract, camellia extract, salviae miltiorrhiza extract, salviae extract, etc., salviae extract, etc, Semen vaccariae extract, gomphrena flower bud/flower extract, gomphrena flower extract, lindera root extract, fig extract, soapberry extract, evodia fruit extract, galla chinensis extract, acanthopanax bark extract, schisandra fruit extract, American ginseng extract, elderberry flower extract, cinnamomum zeylanicum bark extract, leonurus tenuifolia extract, senna angustifolia leaf extract, selfheal extract, opuntia chinensis extract, cactus extract, citrus junos peel extract, citronella extract, bergamot fruit extract, bergamot leaf extract, fructus forsythiae extract, wheat extract, apricot extract, bearberry extract, spiraea ulmaria extract, usnea extract, cynanchum paniculatum extract, figwort extract, inula flower extract, saussurea involucrate extract, nettle extract, mushroom extract, bacillus/soybean fermentation product extract, bacillus subtilis extract, saponarius officinalis extract, myrtle extract, myrcia extract, and the like, Bacillus/rice bran extract/soybean extract fermentation product filtrate, oat extract, sage herb leaf extract, rhubarb horsetails extract, polygonatum rhizome/root extract, pellitory herb extract, wild soybean extract, crataegus fruit extract, motherwort extract, starwort root extract, ginkgo nut extract, ginkgo biloba leaf extract, epimedium extract, neem extract, commiphora mukul resin extract, cherry leaf extract, chestnut seed extract, camellia oleifera leaf extract, camellia oleifera seed extract, pomelo peel extract, pomelo seed extract, houttuynia cordata extract, spiraea ulmaria flower extract, corn poppy flower extract, cinnamon extract, polygonatum extract, guaiacum extract, coriander fruit/leaf extract, peppermint leaf extract, angelica, polygala tenuifolia extract, cinnamon bark extract, ginkgo biloba extract, ginkgo leaf extract, ginkgo biloba extract, sage root extract, Evening primrose extract, Vietnam benzoin resin extract, saussurea involucrate root extract, Yunnan coptis extract, jujube extract, gleditsia sinensis extract, spina gleditsiae extract, quillaja bark extract, rhizoma alismatis extract, camphor tree leaf extract, longstalck peach leaf extract, selfheal extract, jonquil flower extract, rheum palmatum extract, Zhejiang horse chestnut extract, pearl extract, sesame extract, rhizoma anemarrhenae extract, gardenia extract, sessile stemona root extract, Chinese rehmannia root extract, umbellate pore fungus extract, panax japonicus extract, zanthoxylum bungeanum extract, lithospermum extract, mirabilis jalapa extract, perilla extract, rosewood extract, wisteria extract, ganoderma extract and palm extract.
7. The plant extract composite bacteriostatic agent as claimed in claim 6, wherein the synergistic agent is one or more of Schisandra chinensis extract, levulinic acid and Schizophyllum commune fermentation product extract.
8. The plant extract composite bacteriostatic agent according to claim 1, wherein the solvent is one or a mixture of more than one of 1, 2-propanediol, 1, 3-propanediol, 1, 2-butanediol, 1, 3-butanediol, 1, 4-butanediol, 1, 2-pentanediol, 1, 5-pentanediol, isoprene glycol, 1, 2-hexanediol, 1, 6-hexanediol, ethanol, menthol, laurocapram, water, glycerol and methyl propanediol.
9. The use of the plant extract composite bacteriostatic agent according to claim 1 in the preparation of cosmetics, wherein the cosmetics comprise the plant extract composite bacteriostatic agent, and the cosmetics are selected from any one of facial mask liquid, lotion, cream, toner, essence, stock solution, face cleanser, astringent, perfume, makeup remover, foundation solution, foundation cream, concealer, rouge, lipstick, eye shadow and blush.
10. The use of the plant extract composite bacteriostatic agent in the preparation of cosmetics according to claim 9, wherein the amount of the plant extract composite bacteriostatic agent is 0.5-5% by mass.
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