CN117229862A

CN117229862A - Perfumed soap with mite-removing and bacteria-inhibiting effects and preparation method thereof

Info

Publication number: CN117229862A
Application number: CN202311202979.6A
Authority: CN
Inventors: 杨桐卫
Original assignee: Guangzhou Huaxin Plastic Product Co ltd
Current assignee: Guangzhou Huaxin Plastic Product Co ltd
Priority date: 2023-09-18
Filing date: 2023-09-18
Publication date: 2023-12-15
Also published as: CN118440784A

Abstract

The application discloses a perfumed soap with mite-removing and bacteriostasis effects and a preparation method thereof, wherein the perfumed soap with mite-removing and bacteriostasis effects comprises the following components in parts by weight: 5-10 parts of sargassum fusiforme extract, 3-8 parts of magnolia officinalis extract, 1-5 parts of tree peony root bark extract and 2-6 parts of radix sanguisorbae extract; the sargassum fusiforme extract, the magnolia bark extract, the tree peony root bark extract and the radix sanguisorbae extract are compounded in a specific proportion, so that a synergistic inhibition effect on staphylococcus aureus and bacillus subtilis can be achieved, and the damage of bacteria to human bodies can be reduced. And the polyglutamic acid with the relative molecular weight of 40-70 ten thousand is added, so that the problems of durability and cracking of the soap caused by the addition of glycerol are solved.

Description

Perfumed soap with mite-removing and bacteria-inhibiting effects and preparation method thereof

Technical Field

The application belongs to the field of daily chemicals, and particularly relates to a perfumed soap with mite-removing and bacteria-inhibiting effects and a preparation method thereof.

Background

The soap has strong cleaning ability and low price, and becomes a necessity for people to live. Along with the improvement of the living standard of people, the types of perfumed soaps are more and more, and the functions are also continuously increased. Aiming at the demands of different consumers, the perfumed soap has a multifunctional product, such as a product with the functions of resisting bacteria, softening, moisturizing, removing acnes, refreshing skin, whitening, lasting fragrance and the like, is deeply favored by the consumers.

In our life, a great number of pathogenic bacteria and fungi exist, skin is a main parasitic part of the pathogenic bacteria, and the use of perfumed soap with antibacterial effect is one of good measures for preventing infection. The perfumed soap sold in the market at present basically contains chemical substances such as triclocarban or triclosan, the safety of the chemical substances such as triclocarban or triclosan is to be explored, and the bacteria is easy to cause drug resistance after long-term use of triclocarban or triclosan for bacteriostasis. Some manufacturers use sodium fatty acid and other surfactants as main raw materials to prepare perfumed soaps, and then add quality improvers and appearance improvers to prepare the perfumed soaps after processing and shaping. However, the prepared perfumed soap is generally high in pH value, and is easy to cause damage to skin after long-term use, so that the perfumed soap is generally used for cleaning clothes. Therefore, the mild perfumed soap with the antibacterial effect has certain market value.

Disclosure of Invention

Aiming at the defects in the prior art, the application aims to provide the perfumed soap with the mite-removing and bacteria-inhibiting effects. The sargassum fusiforme extract, the magnolia bark extract, the tree peony root bark extract and the radix sanguisorbae extract are compounded in a specific proportion, so that a synergistic inhibition effect on staphylococcus aureus and bacillus subtilis can be achieved, and the damage of bacteria to human bodies can be reduced. And the polyglutamic acid with the relative molecular weight of 40-70 ten thousand is added, so that the problems of durability and cracking of the soap caused by the addition of glycerol are solved.

The application aims to provide a perfumed soap with mite-removing and bacteria-inhibiting effects, which comprises the following components in parts by weight: 5-10 parts of sargassum fusiforme extract, 3-8 parts of magnolia bark extract, 1-5 parts of tree peony root bark extract and 2-6 parts of radix sanguisorbae extract.

Preferably, the perfumed soap with mite-removing and bacteria-inhibiting effects further comprises the following components in parts by weight: 20-30 parts of olive oil, 10-20 parts of sodium hydroxide and 15-35 parts of water.

The olive oil is rich in squalene and essential fatty acid of human body with excellent skin affinity, and can be absorbed rapidly, so that the skin elasticity and the skin luster can be effectively maintained; the olive oil contains rich monounsaturated fatty acid, vitamin E, K, A, D and other phenol antioxidant substances, can eliminate facial wrinkles, prevent skin aging, has the effects of protecting skin and hair, preventing hand and foot chaps and the like, and is called as a 'eating' beautifying skin care product. However, the olive oil contains unsaturated fatty acid higher than any vegetable oil, and is used for preparing the perfumed soap, so that the perfumed soap is easy to change color and rancid. The inventor has unexpectedly found that the sargassum fusiforme extract, the magnolia bark extract, the tree peony root bark extract and the raw garden burnet extract can improve the problem of color change and rancidity of the soap caused by the addition of olive oil in the test process, and the possible reason is that the compound of the sargassum fusiforme extract, the magnolia bark extract, the tree peony root bark extract and the raw garden burnet extract has good oxidation resistance.

Preferably, the perfumed soap with mite-removing and bacteria-inhibiting effects further comprises the following components in parts by weight: 1-5 parts of glycerol and 10-20 parts of polyglutamic acid.

The glycerol has the effects of moisturizing, nourishing and whitening skin, and can be smeared on the surface of the skin to quickly moisten the skin, form a protective film, lock the moisture of the skin, promote the metabolism of skin cells and lighten the pigmentation of the skin. However, when preparing the soap, excessive glycerol addition can lead to the durability and cracking of the soap, the inventor adds polyglutamic acid with specific molecular weight, the carboxyl in the molecular structure of the polyglutamic acid and the hydroxyl in the glycerol have hydrogen bond action, the polyglutamic acid is highly stretched in the glycerol and is mutually entangled and connected to form a continuous reticular structure, and the glycerol is mutually combined with the polyglutamic acid in the network through polar bonds and hydrogen bonds, so that the problems of the durability and the cracking of the soap caused by adding the glycerol are solved.

Preferably, the relative molecular weight of the polyglutamic acid is 40 to 70 tens of thousands.

The molecular weight of the polyglutamic acid is too small, the acting force with the glycerol is small, a net structure cannot be well formed, and the problems of durability and cracking of the perfumed soap caused by adding the glycerol cannot be well solved; the molecular weight of the polyglutamic acid is too large, the polyglutamic acid is intertwined and connected to form a continuous network structure, carboxyl groups in a molecular chain are wrapped in the network structure and cannot be well contacted with the glycerol, so that the problem that the perfumed soap is not durable and cracked due to the addition of the glycerol cannot be well solved.

Preferably, the extraction process of the sargassum fusiforme extract is as follows:

reflux extracting dried and pulverized Cyrtymenia Sparsa with solvent, centrifuging, and concentrating supernatant to obtain Cyrtymenia Sparsa extract.

Preferably, the solvent is selected from at least one of ethanol, methanol, water.

Preferably, the extraction process of the magnolia bark extract is as follows:

reflux extracting dried and pulverized cortex Magnolia officinalis with solvent, centrifuging, and concentrating supernatant to obtain cortex Magnolia officinalis extract.

Preferably, the solvent is selected from at least one of water and ethanol.

Preferably, the extraction process of the tree peony root bark extract is as follows:

reflux-extracting dried and pulverized cortex moutan with solvent, centrifuging, and concentrating supernatant to obtain cortex moutan extract.

Preferably, the solvent is selected from n-hexane or petroleum ether.

Preferably, the extraction process of the radix sanguisorbae extract is as follows:

reflux-extracting dried and pulverized radix Sangusorbae with solvent, centrifuging, and concentrating supernatant to obtain radix Sangusorbae extract.

Preferably, the solvent is selected from at least one of water and ethanol.

The application also aims at providing a preparation method of the perfumed soap with the mite-removing and bacteria-inhibiting effects, which comprises the following steps:

s1, mixing sodium hydroxide and water, heating to 60-80 ℃, adding sargassum fusiforme extract, magnolia officinalis extract, tree peony root bark extract, raw garden burnet extract and olive oil, and stirring to obtain a mixture A;

s2, mixing and stirring glycerol and polyglutamic acid to obtain a mixture B;

s3, adding the mixture B into the mixture A, and stirring to obtain mixed soap solution;

s4, pouring the mixed soap solution into a mold, sealing, standing, demolding and drying to obtain the perfumed soap with the mite-removing and bacteria-inhibiting effects.

Preferably, in S1, the stirring time is 0.5 to 1 hour.

Preferably, in S2, the temperature of the stirring is 50-60 ℃ and the time is 10-20min.

Preferably, in S3, the temperature of the stirring is 40-50 ℃ and the time is 20-30min.

Preferably, in S4, the sealing temperature is 30-40 ℃ and the sealing time is 20-30min.

Preferably, in S4, the drying temperature is 40-50 ℃ and the drying time is 5-8 hours.

Detailed Description

In order to make the technical solution of the present application better understood by those skilled in the art, the technical solution of the present application will be clearly and completely described in conjunction with the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, shall fall within the scope of the present application.

Example 1: extracting Cyrtymenia Sparsa extract.

Pulverizing dried Cyrtymenia Sparsa 100kg with Chinese medicinal pulverizer, sieving with 80-100 mesh sieve, adding 800mL of anhydrous ethanol, heating and reflux extracting for 2-3 hr, cooling, centrifuging for 15min, filtering to remove insoluble substances, decolorizing the filtrate with active carbon, filtering to remove active carbon, centrifuging, concentrating the supernatant to obtain Cyrtymenia Sparsa extract, and storing in dark place.

Example 2: extracting Magnolia bark extract.

Pulverizing dried Magnolia officinalis 100kg with Chinese medicinal pulverizer, sieving with 40-50 mesh sieve, adding 1000mL of anhydrous ethanol, heating and reflux extracting for 1-1.5 hr, cooling, filtering, mixing filtrates, concentrating under reduced pressure to obtain Magnolia officinalis extract, and storing in dark place.

Example 3: extracting cortex moutan extract.

Pulverizing dried cortex moutan root bark 50kg with a traditional Chinese medicine pulverizer, sieving with 80-100 mesh sieve, adding 500mL n-hexane, reflux extracting at 70-80deg.C for 24 hr, filtering, concentrating the extractive solution under reduced pressure with a rotary evaporator to obtain cortex moutan root bark extract, and preserving in dark place.

Example 4: extracting radix Sangusorbae extract.

Pulverizing dried radix Sangusorbae 50kg with Chinese medicinal pulverizer, sieving with 40-50 mesh sieve, adding 500mL of water, heating and reflux extracting for 1-1.5 hr, cooling, filtering, mixing filtrates, concentrating under reduced pressure to obtain radix Sangusorbae extract, and preserving in dark place.

Example 5: and (3) preparing the perfumed soap.

S1, mixing 20 parts by mass of sodium hydroxide with 15 parts by mass of water, heating to 60-80 ℃, adding 5 parts by mass of sargassum fusiforme extract, 8 parts by mass of magnolia officinalis extract, 1 part by mass of tree peony root bark extract, 6 parts by mass of radix sanguisorbae extract and 26 parts by mass of olive oil, and stirring for 0.5-1 hour to obtain a mixture A;

s2, mixing 3 parts by mass of glycerol with 20 parts by mass of polyglutamic acid with a relative molecular weight of 40 ten thousand, and stirring for 10-20min at 50-60 ℃ to obtain a mixture B;

s3, adding the mixture B into the mixture A, and stirring for 20-30min at 40-50 ℃ to obtain mixed soap solution;

s4, pouring the mixed soap solution into a mold, sealing for 20-30min at 30-40 ℃, standing, demolding, and drying for 5-8 hours at 40-50 ℃ to obtain the perfumed soap with the mite-removing and bacteria-inhibiting effects.

Example 6: and (3) preparing the perfumed soap.

S1, mixing 10 parts by mass of sodium hydroxide and 29 parts by mass of water, heating to 60-80 ℃, adding 10 parts by mass of sargassum fusiforme extract, 3 parts by mass of magnolia officinalis extract, 5 parts by mass of tree peony root bark extract, 2 parts by mass of radix sanguisorbae extract and 30 parts by mass of olive oil, and stirring for 0.5-1 hour to obtain a mixture A;

s2, mixing 5 parts by mass of glycerol and 10 parts by mass of polyglutamic acid with the relative molecular weight of 70 ten thousand, and stirring for 10-20min at 50-60 ℃ to obtain a mixture B;

Example 7: and (3) preparing the perfumed soap.

S1, mixing 15 parts by mass of sodium hydroxide with 35 parts by mass of water, heating to 60-80 ℃, adding 7 parts by mass of sargassum fusiforme extract, 6 parts by mass of magnolia officinalis extract, 3 parts by mass of tree peony root bark extract, 4 parts by mass of radix sanguisorbae extract and 20 parts by mass of olive oil, and stirring for 0.5-1 hour to obtain a mixture A;

s2, mixing 1 part by mass of glycerol with 13 parts by mass of polyglutamic acid with a relative molecular weight of 40 ten thousand, and stirring for 10-20min at 50-60 ℃ to obtain a mixture B;

Comparative example 1: and (3) preparing the perfumed soap.

S1, mixing 15 parts by mass of sodium hydroxide with 35 parts by mass of water, heating to 60-80 ℃, adding 20 parts by mass of sargassum fusiforme extract and 20 parts by mass of olive oil, and stirring for 0.5-1 hour to obtain a mixture A;

Comparative example 2: and (3) preparing the perfumed soap.

S1, mixing 15 parts by mass of sodium hydroxide with 35 parts by mass of water, heating to 60-80 ℃, adding 20 parts by mass of magnolia bark extract and 20 parts by mass of olive oil, and stirring for 0.5-1 hour to obtain a mixture A;

Comparative example 3: and (3) preparing the perfumed soap.

S1, mixing 15 parts by mass of sodium hydroxide with 35 parts by mass of water, heating to 60-80 ℃, adding 20 parts by mass of cortex moutan extract and 20 parts by mass of olive oil, and stirring for 0.5-1 hour to obtain a mixture A;

Comparative example 4: and (3) preparing the perfumed soap.

S1, mixing 15 parts by mass of sodium hydroxide with 35 parts by mass of water, heating to 60-80 ℃, adding 20 parts by mass of a raw garden burnet extract and 20 parts by mass of olive oil, and stirring for 0.5-1 hour to obtain a mixture A;

Test example 1: and (5) testing a bacteriostasis area.

(1) Test strain: coli ATCC8099, staphylococcus aureus ATCC6538, bacillus subtilis ATCC55614, all of which are all from the Guangdong province microorganism strain collection center.

(2) Preparation of the culture medium: 10g of the solid nutrient broth was dissolved in 500mL of purified water and autoclaved at 121℃for 20min for further use.

(3) Preparation of bacterial suspension: sterilizing the test tube and culture medium at 121deg.C for 30min, adding 5mL of culture medium into the 3 sterilized test tubes, cooling the culture medium to below 40deg.C, inoculating appropriate amount of Escherichia coli, staphylococcus aureus and Bacillus subtilis, respectively placing into the 3 test tubes, mixing, culturing in 37 deg.C incubator for 18-24 hr, and regulating bacterial liquid turbidity with Mitsubishi turbidimeter to 10% bacterial suspension ⁵ CFU/mL, ready for use.

(4) Preparation of test samples: the soaps prepared in examples 5-7 and comparative examples 1-4 were placed in conical flasks, 5g each, and 25mL of sterile distilled water was added, and the soaps were shaken in a water bath at 45℃until they dissolved, to obtain test samples;

(5) Determination of antibacterial properties: 100 mu L of bacterial suspension is sucked into a culture dish containing a solid nutrient broth culture medium, uniformly coated by a distributor, an oxford cup is clamped by sterile forceps and placed on the nutrient broth culture medium coated with uniform bacterial liquid, the oxford cup is fully contacted with the culture medium, and 3 repeated experiments are carried out on each strain. After 50. Mu.L of sample is added into each oxford cup, the petri dishes are placed in an incubator, staphylococcus aureus, escherichia coli and bacillus subtilis are placed in the incubator at 37 ℃ for culturing for 18-24 hours, the size of a bacteriostasis zone is observed, and the diameter of the bacteriostasis zone is measured by a ruler.

Table 1. Results of inhibition zone test.

As can be seen from Table 1, the toilet soaps of examples 5 to 7 of the present application have synergistic antibacterial effects against Staphylococcus aureus and Bacillus subtilis, but have no remarkable synergistic antibacterial effects against Escherichia coli.

Test example 2: measurement of DPPH radical scavenging Rate.

40mg of DPPH is weighed, 1000mL of methanol is added for dissolution, 0.04mg/mL of DPPH methanol solution is obtained, and the perfumed soaps of examples 5-7 and comparative examples 1-4 are respectively prepared into sample solutions with the concentration of 0.5 mg/mL. To 2mL of each sample solution was added 2mL of methanol solution of LDPPH, and the reaction was carried out in the dark for 30min, and the absorbance at 517nm was measured. Distilled water is used as a blank instead of a sample, a methanol solution is used as a sample blank instead of a DPPH methanol solution, and vitamin C is used as a positive control.

The clearance of the sample to DPPH radicals was calculated using the following formula:

DPPH radical scavenging = [1- (A1-A2)/A0 ] ×100%;

blank A0: absorbance of 2mL distilled water+2 mL dph solution;

sample A1: absorbance of 2mL sample solution+2 mL dpph solution;

sample blank A2: absorbance of 2mL sample solution+2 mL methanol solution.

Table 2. Test results of the soaps of examples 5-7 and comparative examples 1-4 for DPPH radical scavenging rate.

Sample of	DPPH radical scavenging Rate (%)
		Example 5	85.4
Example 6	86.3
		Example 7	87.8
Comparative example 1	72.4
		Comparative example 2	59.1
Comparative example 3	75.9
		Comparative example 4	68.7

From Table 2, it is clear that the combination of Cyrtymenia Sparsa extract, magnolia cortex extract, cortex moutan extract and radix Sangusorbae extract can effectively solve oxidative spoilage and discoloration problems of olive oil.

Test example 3: and (5) accelerating the measurement of the color-changing spoilage.

The soaps of examples 5 to 7 and comparative examples 1 to 4 were placed in an electrically heated constant temperature incubator at 40.+ -. 1 ℃ for 24 hours, and after the room temperature was restored, the appearance of discoloration and spoilage was observed.

Table 3. Test results of the color-changing spoilage properties of the soaps of examples 5-7 and comparative examples 1-4.

Sample of	Color change spoilage condition
		Example 5	No discoloration and spoilage
Example 6	No discoloration and spoilage
		Example 7	No discoloration and spoilage
Comparative example 1	Slightly discolored spoilage
		Comparative example 2	Slightly discolored spoilage
Comparative example 3	Slightly discolored spoilage
		Comparative example 4	Slightly discolored spoilage

From Table 3, it is clear that the combination of Cyrtymenia Sparsa extract, magnolia cortex extract, cortex moutan extract and radix Sangusorbae extract can effectively solve oxidative spoilage and discoloration problems of olive oil.

Comparative example 5: and (3) preparing the perfumed soap.

s2, adding 1 part by mass of glycerol into the mixture A, and stirring for 20-30min at 40-50 ℃ to obtain mixed soap solution;

s3, pouring the mixed soap solution into a mold, sealing for 20-30min at 30-40 ℃, standing, demolding, and drying for 5-8 hours at 40-50 ℃ to obtain the perfumed soap with the mite-removing and bacteria-inhibiting effects.

Comparative example 6: and (3) preparing the perfumed soap.

s2, mixing 1 part by mass of glycerol with 13 parts by mass of polyglutamic acid with a relative molecular weight of 100 ten thousand, and stirring for 10-20min at 50-60 ℃ to obtain a mixture B;

Comparative example 7: and (3) preparing the perfumed soap.

s2, mixing 1 part by mass of glycerol with 13 parts by mass of polyglutamic acid with a relative molecular weight of 1 ten thousand, and stirring for 10-20min at 50-60 ℃ to obtain a mixture B;

Test example 4: stability testing.

The soaps of examples 5 to 7 and comparative examples 5 to 7 were placed under a faucet, respectively, the surfaces of the soap body were scrubbed with a towel, each surface was scrubbed, the soaps were placed at room temperature for 24 hours after scrubbing, and after 10 times of such scrubbing, the appearance was observed for cracks, and the results are shown in table 4.

Table 4. Quality test results of soaps of examples 5-7 and comparative examples 5-7.

As can be seen from Table 4, the soaps prepared in examples 5 to 7, supplemented with polyglutamic acid having a relative molecular weight of 40 to 70 tens of thousands, can improve the problem of durability and cracking caused by the addition of glycerin.

Finally, it should be noted that the above-mentioned embodiments are only for illustrating the technical solution of the present application and not for limiting the same, and although the present application has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the specific embodiments of the present application after reading the present specification, and these modifications and variations do not depart from the scope of the application as claimed in the pending claims.

Claims

1. The perfumed soap with the mite-removing and bacteria-inhibiting effects is characterized by comprising the following components in parts by weight: 5-10 parts of sargassum fusiforme extract, 3-8 parts of magnolia bark extract, 1-5 parts of tree peony root bark extract and 2-6 parts of radix sanguisorbae extract.

2. The perfumed soap with mite-removing and bacteria-inhibiting effects as claimed in claim 1, further comprising the following components in parts by weight: 20-30 parts of olive oil, 10-20 parts of sodium hydroxide and 15-35 parts of water.

3. The perfumed soap with mite-removing and bacteria-inhibiting effects as claimed in claim 1, further comprising the following components in parts by weight: 1-5 parts of glycerol and 10-20 parts of polyglutamic acid.

4. A toilet soap with mite-killing and bacteria-inhibiting effects according to claim 3, wherein the relative molecular weight of the polyglutamic acid is 40-70 ten thousand.

5. The perfumed soap with mite-removing and bacteria-inhibiting effects according to claim 1, wherein the extraction process of the sargassum fusiforme extract is as follows:

6. The perfumed soap with mite-removing and bacteria-inhibiting effects as claimed in claim 1, wherein the extraction process of the magnolia bark extract is as follows:

7. The perfumed soap with mite-removing and bacteria-inhibiting effects as claimed in claim 1, wherein the extraction process of the tree peony root bark extract is as follows:

8. The toilet soap with mite-removing and bacteria-inhibiting effects according to claim 7, wherein the solvent is selected from n-hexane or petroleum ether.

9. The perfumed soap with mite-removing and bacteria-inhibiting effects according to claim 1, wherein the extraction process of the radix sanguisorbae extract is as follows:

10. The method for preparing the perfumed soap with mite-removing and bacteria-inhibiting effects according to any one of claims 1 to 9, which is characterized by comprising the following steps:

s2, mixing and stirring glycerol and polyglutamic acid to obtain a mixture B;