CN114748380A - Composition containing marine plant extract and application thereof - Google Patents

Composition containing marine plant extract and application thereof Download PDF

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CN114748380A
CN114748380A CN202210508925.1A CN202210508925A CN114748380A CN 114748380 A CN114748380 A CN 114748380A CN 202210508925 A CN202210508925 A CN 202210508925A CN 114748380 A CN114748380 A CN 114748380A
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extract
marine plant
plant extract
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CN114748380B (en
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刘倩
梅欢
崔健
张代雨
李季阳
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Jiangsu University of Science and Technology
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706Algae
    • A61K8/9711Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00General protective or antinoxious agents
    • A61P39/06Free radical scavengers or antioxidants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00Make-up preparations; Body powders; Preparations for removing make-up
    • A61Q1/12Face or body powders for grooming, adorning or absorbing
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • AHUMAN NECESSITIES
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    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
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    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/10Washing or bathing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
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    • AHUMAN NECESSITIES
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    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/82Preparation or application process involves sonication or ultrasonication
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention discloses a composition containing a marine plant extract and application thereof, wherein the composition containing the marine plant extract comprises the following components in percentage by mass: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract. By compounding the sargassum fusiforme extract, the centella extract, the artemisia capillaries extract and the black soybean extract in a specific ratio, the antibacterial performance and the antioxidant performance can be obviously improved, the quick and long-acting antibacterial effect is achieved, and the composition has the advantages of low dosage, safety, no stimulation and the like when being used in cosmetics.

Description

Composition containing marine plant extract and application thereof
Technical Field
The invention belongs to the field of bacteriostasis, and particularly relates to a composition containing a marine plant extract and application thereof.
Background
The marine plant resources of China are very rich, various animals, plants and microorganisms live in the sea, the marine organisms have different metabolic pathways from terrestrial organisms due to special environments such as high pressure and high salt of the sea, the components contained in many marine plants have specific chemical structures and wide pharmacological activities, such as halogenated terpenoids, polyphenols, neurotoxin, polysaccharide, polypeptide and the like, the main pharmacological effects of the marine plants comprise antibiosis, anti-inflammation, antioxidation, antitumor, antivirus, cardiovascular disease treatment, immune regulation and the like, most of the marine plants have good effects of antibiosis, antivirus, anti-inflammation, antioxidation, antitumor, cardiovascular disease treatment, immune regulation and the like, and the clinical development and application prospect is wide.
The marine plants have rich nutritional value, and have the following nutritional characteristics: 1. the crude protein content is high, and the feed contains rich vitamins and mineral substances, thereby being beneficial to the breeding, growth and development of livestock and poultry. 2. Contains antibacterial and bacteriostatic active substances. The active substances have inhibiting effect on mould, Staphylococcus aureus, Escherichia coli, salmonella, etc., and can improve immunity and disease resistance of livestock and fowl. 3. Contains bioactive hormone and somatomedin. Can regulate the balance of various nutrients in the feed, promote the digestion and absorption of nutrient substances, generate an anti-stress effect, and improve the growth speed and the disease resistance of the livestock and poultry. 4. It is rich in pigment. The marine plant contains phycoxanthin, carotene, etc.; when the feed is used in livestock and poultry feed, the quality of livestock and poultry products can be obviously improved, and the skin and muscle of livestock and poultry are bright in color. 5. High iodine content and proper calcium-phosphorus ratio. The feed is used for preparing livestock and poultry feed, so that the meat, egg and milk products are rich in nutrition and delicious in taste; the thickness of the eggshell is increased, and the color of the yolk is changed into dark yellow; especially, the iodine content in the egg yolk is dozens of times higher than that in the original egg yolk; the fat structure is also changed, and the content of cholesterol is reduced. 6. Contains phenol compounds, natural gum, alginic acid, etc. The compound containing phenol has strong bacteriostasis and mildew-proof function, is a natural feed mildew preventive, contains agar, alginic acid and water-absorbing substances, is a natural feed adhesive and a moisture-proof agent, and can absorb water in the feed. 7. The brown algae starch with the functions of reducing blood fat and resisting coagulation is iodized to obtain the sulfate which can replace heparin and has the functions of reducing blood fat, resisting coagulation and improving a microcirculation system.
Cyrtymenia Sparsa is a marine plant belonging to algae, and is mainly distributed in Zhejiang and Fujian provinces in China. The sargassum fusiforme extract contains abundant polysaccharide, dietary cellulose, B vitamins, alginic acid and mineral substances and trace elements necessary for human bodies, has good biological activity, but has poor antibacterial and antioxidant properties. Therefore, the improvement of the antibacterial performance and the antioxidant performance of the sargassum fusiforme extract has important significance.
Disclosure of Invention
In view of the above-mentioned deficiencies in the prior art, the present invention aims to provide a composition containing marine plant extract, which comprises the following components by mass: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract. By compounding the sargassum fusiforme extract, the centella extract, the artemisia capillaries extract and the black soybean extract in a specific ratio, the components have a synergistic interaction effect, so that the antibacterial performance and the antioxidant performance can be remarkably improved, and the quick and long-acting antibacterial effect is achieved. The composition containing marine plant extract can be used in cosmetics, and has the advantages of low dosage, safety, and no irritation.
The invention aims to provide a composition containing a marine plant extract, which comprises the following components in percentage by mass: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract.
Sargassum fusiforme is a marine plant belonging to algae, also known as antler tip, seaweed bud, goat milk seed, sea barley, etc. Onychidae, Strongylocentrotus. The Cyrtymenia Sparsa has rich nutrition. The fresh Cyrtymenia Sparsa contains water about 83.16%, and contains protein, carbohydrate, ash and fiber 15%, 46%, 30% and 7% in dry basis, and is a natural food with low fat and calorie, and has high nutritive value, and can be used as vegetable or medicine. The Cyrtymenia Sparsa extract is rich in polysaccharide, dietary cellulose, B vitamins, alginic acid and essential minerals and microelements, and has antibacterial and antioxidant effects.
Centella asiatica, perennial herbs, stems crawl, thin and long, and roots on the nodes. Centella asiatica extract contains asiatic acid and madecassic acid, and has effects of inhibiting Staphylococcus aureus and resisting oxidation.
Herba Artemisiae Scopariae is Compositae, Artemisia semi-shrub-like herbaceous plant, and the plant has strong fragrance. The herba Artemisiae Scopariae extract contains volatile oil components, vitamins, mineral components and terpenoids, has antiinflammatory, antibacterial and antioxidant effects, and can inhibit various free radical activities in skin cells, relieve skin aging, restore skin activity, and relieve skin aging with antiinflammatory and antibacterial effects.
Semen Sojae Atricolor is mature seed of Leguminosae plant, and is the main raw material of traditional Chinese medicine semen Sojae Preparatum. The semen Sojae Atricolor extract contains bioactive components such as soybean isoflavone, anthocyanin and tannin, and has various biological activities of relieving climacteric syndrome, resisting oxidation, protecting cardiovascular system, resisting cancer, bacteria and virus.
The inventor finds that the composition prepared by compounding the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract in a specific ratio has synergistic antibacterial and antioxidant effects among the components, and the probable reason is that the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract all contain hydroxyl compounds, hydrogen bonding effects exist among the hydroxyl compounds, and the active components are mutually coordinated and promoted to have good synergistic effects.
Preferably, the composition containing the marine plant extract comprises the following components in percentage by mass: 20% of sargassum fusiforme extract, 10% of centella asiatica extract, 10% of artemisia capillaris extract and 20% of black soybean extract.
Preferably, the extraction process of the sargassum fusiforme extract is as follows:
extracting dried and pulverized Cyrtymenia Sparsa with solvent under reflux, centrifuging, and concentrating the supernatant to obtain Cyrtymenia Sparsa extract.
Preferably, the solvent is selected from one or two of ethanol and methanol.
Preferably, the extraction process of the centella asiatica extract is as follows:
mixing dried herba Centellae and deionized water, soaking, boiling, separating to obtain medicinal liquid, adding deionized water into the residue, boiling, mixing the two boiled medicinal liquids, filtering, centrifuging, and concentrating the supernatant to obtain herba Centellae extract.
Preferably, the extraction process of the artemisia capillaris extract is as follows:
mixing dried and sieved herba Artemisiae Scopariae powder with the extractive solution, leaching, ultrasonic extracting, decolorizing, filtering, and concentrating to obtain herba Artemisiae Scopariae extract.
Preferably, the extract is selected from a mixed solvent of water and ethanol with a volume ratio of 1: 9; the temperature of the leaching is 60-70 ℃.
Preferably, the extraction process of the black soybean extract is as follows:
mixing dried and sieved semen Sojae Atricolor powder with extractive solution, leaching, adjusting pH, ultrasonic extracting, filtering, decolorizing, filtering, and concentrating the filtrate to obtain herba Artemisiae Scopariae extract.
Preferably, the extract is selected from a mixed solvent of water and butanediol with the volume ratio of 2: 8; the leaching temperature is 40-50 ℃; the pH value is 4.0-5.0.
Preferably, the composition containing the marine plant extract comprises 10-70% by mass of a solvent.
Preferably, the solvent is selected from one or more of ethanol, 1, 2-propanediol, 1, 3-propanediol, 1, 2-butanediol, 1, 3-butanediol, 1, 4-butanediol, 1, 2-pentanediol, 1, 5-pentanediol, isoprene glycol, 1, 2-hexanediol, 1, 6-hexanediol, water, glycerol.
Another object of the present invention is to provide a method for preparing a composition containing marine plant extracts, comprising the steps of:
mixing Cyrtymenia Sparsa extract 10-30%, herba Centellae extract 5-15%, herba Artemisiae Scopariae extract 5-15%, semen Sojae Atricolor extract 10-30% and solvent 10-70%, stirring, heating to 45-55 deg.C, cooling, and packaging.
The invention also aims to provide application of the composition containing the marine plant extract in preparing cosmetics, wherein the cosmetics contain the composition containing the marine plant extract.
Preferably, the cosmetic is selected from any one of a facial solution, a lotion, a cream, a essence, a stock solution, a face cleanser, a foundation solution, a foundation cream and a concealer.
Preferably, the composition containing the marine plant extract accounts for 0.1-0.5% of the weight of the cosmetic.
Preferably, the composition containing the marine plant extract accounts for 0.5 percent of the weight of the cosmetic.
Compared with the prior art, the invention has the following beneficial effects:
(1) the composition containing the marine plant extract provided by the invention is safe and non-irritant because each component is derived from natural plants, and has good synergistic effect because each active component is mutually coordinated and promoted.
(2) According to the invention, the sargassum fusiforme extract, the centella extract, the artemisia capillaries extract and the black soybean extract are compounded in a specific ratio, so that the antibacterial performance and the antioxidant performance of the composition can be obviously improved, and the quick and long-acting antibacterial effect is achieved.
(3) The composition containing the marine plant extract prepared by the invention can achieve high-efficiency antibacterial and antioxidant effects at a lower dosage.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the following will clearly and completely describe the technical solution of the present invention with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: extracting Cyrtymenia Sparsa.
Pulverizing dried Cyrtymenia Sparsa 100kg, sieving with 50 mesh sieve, extracting with 500mL 95% ethanol at 78 deg.C for 2-3 hr, centrifuging for 10min, filtering to remove insoluble substances, decolorizing the filtrate with activated carbon, filtering to remove activated carbon, centrifuging, and concentrating the supernatant to obtain Cyrtymenia Sparsa extract.
Example 2: and (4) extracting centella asiatica.
Crushing 100kg of dried centella, sieving with a 100-mesh sieve, adding 500mL of deionized water, soaking for 10h, heating and boiling, separating out liquid medicine, adding 500mL of deionized water into dregs of a decoction, heating and boiling, combining the liquid medicine after twice boiling, filtering to remove insoluble substances, adding activated carbon into filtrate for decolorization, filtering to remove the activated carbon, centrifuging, and concentrating supernatant to obtain the centella extract.
Example 3: extracting herba Artemisiae Scopariae.
Pulverizing 100kg of dried herba Artemisiae Scopariae, sieving with 50 mesh sieve, adding 1000mL of mixed solvent of water and ethanol at volume ratio of 1:9, extracting at 60-70 deg.C for 3-5 hr, ultrasonic extracting, decolorizing the filtrate with activated carbon, filtering to remove activated carbon, and concentrating the filtrate to obtain herba Artemisiae Scopariae extract.
Example 4: extracting black soybean.
Crushing 100kg of dried black soybean, sieving with a 50-mesh sieve, adding 1000mL of a mixed solvent of water and butanediol with the volume ratio of 2:8, leaching at 40-50 ℃ for 3-4 hours, adjusting the pH value to 4.0-5.0, performing ultrasonic extraction, filtering, adding activated carbon into the filtrate for decolorization, filtering, and concentrating the filtrate to obtain the black soybean extract.
Example 5: preparation method of composition containing marine plant extract is provided.
Mixing Cyrtymenia Sparsa extract 20g, herba Centellae extract 10g, herba Artemisiae Scopariae extract 10g, semen Sojae Atricolor extract 20g and 1, 3-butanediol 40g, stirring, heating to 50 deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain composition containing marine plant extract.
Example 6: preparation method of composition containing marine plant extract is provided.
Mixing Cyrtymenia Sparsa extract 10g, herba Centellae extract 15g, herba Artemisiae Scopariae extract 5g, semen Sojae Atricolor extract 30g and 1, 3-butanediol 40g, stirring, heating to 50 deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain composition containing marine plant extract.
Example 7: preparation method of composition containing marine plant extract is provided.
Mixing Cyrtymenia Sparsa extract 30g, herba Centellae extract 5g, herba Artemisiae Scopariae extract 15g, semen Sojae Atricolor extract 10g and 1, 3-butanediol 40g, stirring, heating to 50 deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain composition containing marine plant extract.
Comparative example 1: preparation of a one-component composition.
Mixing Cyrtymenia Sparsa extract 60g and 1, 3-butanediol 40g, stirring, heating to 50 deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain single component composition.
Comparative example 2: preparation of a one-component composition.
Mixing herba Centellae extract 60g and 1, 3-butanediol 40g, stirring, heating to 50 deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain single component composition.
Comparative example 3: preparation of a one-component composition.
Mixing herba Artemisiae Scopariae extract 60g and 1, 3-butanediol 40g, stirring, heating to 50 deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain single component composition.
Comparative example 4: preparation of a one-component composition.
Mixing 60g of black soybean extract and 40g of 1, 3-butanediol, stirring, heating to 50 ℃, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain the single-component composition.
Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC):
preparation of liquid medium: weighing 10g of peptone, 10g of glucose, 5g of sodium chloride and 5g of beef extract, adding water, boiling to fully dissolve all the components, fixing the volume to 1000mL, cooling to the normal temperature, adjusting the pH value to be neutral, placing the culture medium under the conditions of 0.1MPa and 120 ℃, sterilizing for 30min, cooling to the normal temperature, and placing in a refrigerator at 4 ℃ for storage for later use.
(2) Preparation of bacterial suspension:
strain: staphylococcus aureus ATCC6538 from the Guangdong province culture Collection of microorganisms.
Preparation of bacterial suspension: inoculating Staphylococcus aureus in logarithmic growth phase in liquid culture medium, culturing at 37 deg.C for 12 hr, concocting into bacterial suspension with 0.5 McLeod turbidity with sterile liquid culture medium, diluting at 1:1000 ratio until the bacteria content is about 105CFU/mL。
(3) Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC).
A two-fold dilution method is adopted to search a proper bacteriostatic and bactericidal concentration range: staphylococcus aureus in logarithmic growth phase was inoculated into liquid medium (suspension concentration 1X 10)5CFU/mL), equal amounts of the compositions of examples 5-7 and comparative examples 1-4 were added at different concentrations, respectively, so that the concentrations of the compositions of examples 5-7 and comparative examples 1-4 in the medium were: 160. 80, 40, 20, 10, 5 and 2.5mg/mL, carrying out shake culture at 37 ℃ and 120r/min for 24h, observing growth conditions, and recording the lowest concentration of bacteria-free growth in the test tube to determine a proper bacteriostatic concentration range.
Determining the minimum bacteriostatic concentration and the minimum bactericidal concentration by adopting a gradient method: staphylococcus aureus in logarithmic growth phase was inoculated into liquid medium (suspension concentration 1X 10)5CFU/mL), equal amounts of the compositions of examples 5-7 and comparative examples 1-4 were added at different concentrations, respectively, so that the concentrations of the compositions of examples 5-7 and comparative examples 1-4 in the medium were: the concentration determined by a double dilution method is tested according to a concentration gradient of 0.5mg/mL,
Meanwhile, sterile water and 1, 3-butanediol are respectively used as blank control groups, and the minimum dilution concentration of the liquid culture medium with clear color is the minimum bactericidal concentration (MIC) after shaking culture at 37 ℃ and 120r/min for 24 h. And coating the bacteria liquid on a flat plate, observing the growth condition of the bacteria, and obtaining the minimum dilution concentration at which the bacteria do not grow as the MBC value.
TABLE 1 MIC and MBC determination results for the compositions of examples 5-7 and comparative examples 1-4.
Figure BDA0003638508950000061
Figure BDA0003638508950000071
As can be seen from Table 1, the marine plant extract-containing composition prepared by compounding the Cyrtymenia Sparsa extract, the centella asiatica extract, the Artemisia capillaris extract and the black soybean extract has a synergistic bacteriostatic effect among the components.
Determination of DPPH radical scavenging Rate:
40mg of DPPH was weighed and dissolved in 1000mL of methanol to obtain a 0.04mg/mL DPPH methanol solution, and sample solutions of 0.5mg/mL were prepared in examples 5 to 7 and comparative examples 1 to 4, respectively. 2mL of DPPH methanol solution was added to 2mL of each sample solution, and the mixture was reacted for 30min in the dark, and the absorbance was measured at 517 nm. Distilled water is used as a blank to replace a sample, a methanol solution is used as a blank to replace a DPPH methanol solution, and vitamin C is used as a positive control.
The DPPH radical clearance rate of the sample is calculated by the following formula:
DPPH free radical scavenging rate ═ 1- (a1-a2)/a0 × 100%;
blank a 0: 2mL of distilled water +2mL of DPPH solution;
sample a 1: 2mL of sample solution +2mL of DPPH solution;
sample blank a 2: absorbance of 2mL sample solution +2mL methanol solution.
TABLE 2 test results for DPPH radical scavenging rate for compositions of examples 5-7 and comparative examples 1-4.
Sample (I) DPPH radical scavenging ratio (%)
Example 5 86.0
Example 6 83.6
Example 7 82.4
Comparative example 1 64.2
Comparative example 2 50.6
Comparative example 3 67.8
Comparative example 4 63.7
Positive control group 40.3
As can be seen from table 2, the marine plant extract-containing compositions prepared by compounding the hijiki extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract all have better antioxidant effects than single components, thus indicating that the marine plant extract-containing compositions prepared by compounding the hijiki extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract have synergistic antioxidant effects.
The formula composition and the preparation method of the essence are as follows:
table 3 formula composition and amount (kg) of the essence.
Figure BDA0003638508950000081
Mixing propylene glycol, glycerol, 1, 2-hexanediol, allantoin, ethylhexylglycerin and water, stirring to dissolve, adding the compositions prepared in examples 5-7 and comparative examples 1-4 respectively, adding carbomer and xanthan gum, and stirring to dissolve to obtain essence.
Corrosion challenge experiments:
experimental strains: the bacterium is staphylococcus aureus ATCC6538, the fungus is Aspergillus niger ATCC16404, and the bacterium and the fungus are all from Guangdong province microorganism strain collection center.
The test method comprises the following steps: with reference to CTFA challenge experiment requirements, 5mL of the essence prepared in examples 8-10 and comparative examples 5-8 were inoculated with 1.0X 10 of Staphylococcus aureus6CFU/g (CFU/mL) and Aspergillus niger 1.0X 104CFU/g (CFU/mL), the inoculated sample was separately tested at a specific time.
And (3) judging test results: results at 0 hours (i.e., immediate post inoculation sampling), 7 days, 14 days, 21 days, and 28 days required 99.9% reduction in bacteria and 90% reduction in fungi at day 7; and continues to decline over 28 days, the preservative challenge test is passed. The results are shown in Table 4.
Table 4. results of preservation challenge test of the essences prepared in examples 8 to 10 and comparative examples 5 to 8.
Figure BDA0003638508950000091
As can be seen from table 4, the essences prepared from the compositions containing marine plant extracts of examples 5 to 7 all passed the preservation challenge test and had strong preservation effects; whereas the essences prepared from the one-component compositions of comparative examples 1-4 all had a decrease in the total number of bacteria and fungi over time, indicating that all had some preservative efficacy, but none passed the preservative challenge test.
Human skin patch experiment:
the purpose of the experiment is as follows: the safety of the essence containing the composition containing marine plant extract prepared in examples 5 to 7 was examined.
The test method comprises the following steps: 100 volunteers are randomly selected according to age composition and gender, and the inclusion and exclusion standards of 2007 cosmetic hygiene standards are met; selecting skin on two sides of upper back vertebra as a tested part, dividing the skin into two parts, wherein one part is a blank control, and the other part is respectively smeared with the essence prepared in the embodiment 8-10; firmly sticking the spot tester adhesive tape added with the spot test substance from bottom to top; the patch test time is 48 h; and after 48 hours after the patch is applied, removing the spot tester, and observing and recording the test result after 30 min.
TABLE 5 skin adverse reaction grading criteria.
Figure BDA0003638508950000092
Figure BDA0003638508950000101
TABLE 6 Patch test results.
Figure BDA0003638508950000102
As can be seen from table 6, the essence of the composition containing marine plant extracts basically reacted negatively to the subjects, and no serious adverse reaction occurred, and the results determined that the essence of the composition containing marine plant extracts did not react adversely to human bodies.
Finally, it should be noted that the above-mentioned embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that after reading the present specification, modifications and equivalents of the specific embodiments of the present invention can be made, but these modifications and variations do not depart from the scope of the claims of the present application.

Claims (10)

1. The composition containing the marine plant extract is characterized by comprising the following components in percentage by mass: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract.
2. The marine plant extract-containing composition according to claim 1, wherein the sargassum fusiforme extract is extracted by the following process: extracting dried and pulverized Cyrtymenia Sparsa with solvent under reflux, centrifuging, and concentrating the supernatant to obtain Cyrtymenia Sparsa extract.
3. The marine plant extract-containing composition according to claim 2, wherein the solvent is one or two selected from the group consisting of ethanol and methanol.
4. The marine plant extract-containing composition according to claim 1, wherein said centella asiatica extract is extracted by the following process: mixing dried herba Centellae with ionized water, soaking, boiling, separating to obtain medicinal liquid, adding ionized water into the residue, boiling, mixing the two boiled medicinal liquids, filtering, centrifuging, and concentrating the supernatant to obtain herba Centellae extract.
5. The marine plant extract-containing composition of claim 1, wherein the artemisia capillaris extract is extracted by the following process: mixing dried and sieved herba Artemisiae Scopariae powder with the extractive solution, leaching, ultrasonic extracting, decolorizing, filtering, and concentrating to obtain herba Artemisiae Scopariae extract.
6. The marine plant extract-containing composition according to claim 5, wherein the extract is selected from the group consisting of a mixed solvent of water and ethanol at a volume ratio of 1: 9; the temperature of the leaching is 60-70 ℃.
7. The marine plant extract-containing composition of claim 1, wherein said black soybean extract is extracted by the following process: mixing dried and sieved semen Sojae Atricolor powder with extractive solution, leaching, adjusting pH, ultrasonic extracting, filtering, decolorizing, filtering, and concentrating the filtrate to obtain herba Artemisiae Scopariae extract.
8. The marine plant extract-containing composition according to claim 7, wherein the extract is selected from the group consisting of a mixed solvent of water and butylene glycol in a volume ratio of 2: 8; the leaching temperature is 40-50 ℃; the pH value is 4.0-5.0.
9. Use of a marine plant extract-containing composition according to any one of claims 1 to 8 in the preparation of a cosmetic, wherein the cosmetic comprises the marine plant extract-containing composition, and the cosmetic is selected from any one of a facial mask solution, an emulsion, a cream, a serum, a stock solution, a face cleanser, a foundation solution, a foundation cream, and a concealer.
10. The marine plant extract-containing composition according to claim 8, wherein the marine plant extract-containing composition is used in an amount of 0.1 to 0.5% by mass.
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