KR20210036767A - Cosmetic composition with sea Lacto for antibacterial and antiinflammatory and manufacturing method thereof - Google Patents

Abstract

The present invention relates to an antibacterial anti-inflammatory sea lacto cosmetic composition, and a method for producing the same. The method comprises the steps of: (a) preparing processed Ecklonia cava extracts, Hizikia fusiforme extracts, kelp extracts, Pyropia yezoensis extracts, brown algae extracts, lactic acid bacteria, and oligosaccharide; (b) mixing 5-10 parts by weight of Hizikia fusiforme extracts, 20-35 parts by weight of kelp extracts, 5-8 parts by weight of Pyropia yezoensis extracts, 5-10 parts by weight of brown algae extracts, 30-90 parts by weight of lactic acid bacteria, and 3-25 parts by weight of oligosaccharide with respect to 100 parts by weight of Ecklonia cava extracts, and agitating the same for 30-45 minutes; (c) fermenting the same for 3-6 months at 30-43℃ and filtering the same to prepare a crude filtrate; and (d) preparing an antibacterial anti-inflammatory sea lactose cosmetic composition by adding 3-50 parts by weight of fucose with respect to 100 parts by weight of the crude filtrate, and stirring the same for 10-12 hours. By using natural ingredients and lactic acid bacteria derived from the sea, it is possible to mass-produce a feminine cleanser with excellent antibacterial and anti-inflammatory effects.

Description

[Cosmetic composition with sea Lacto for antibacterial and antiinflammatory and manufacturing method thereof]

The present invention relates to an antibacterial anti-inflammatory sea lacto cosmetic composition and a method for producing the same.

Republic of Korea Patent Registration No. 1962641 (Registration Date: March 31, 2019, Title of Invention: A composition having antibacterial and antiviral activity containing chestnut gunpox extract as an active ingredient, and a feminine cleansing composition containing the same) is effective in chestnut gunpox extract. Included as a component, wherein the antimicrobial activity is antibacterial activity against Gardnerella vaginalis and Chlamydia trachomatis, and the antiviral activity is against human papillomavirus (HPV). Disclosed is a feminine cleansing composition comprising a composition having antibacterial and antiviral activity as an active ingredient, including in an amount of 0.0001 to 10% by weight with respect to the total weight of the composition in which the chestnut musculus extract has antibacterial and antiviral activity. Has been.

In this prior art, the composition containing the chestnut musculus extract exhibits antimicrobial activity against Gardnerella vaginalis, which causes female vaginitis, inhibits Chlamydia trachomatis infection and inhibits infection from human papillomavirus, It can be usefully used as a composition having the effect of preventing diseases from infection from, and claims that it can be safely used because there are few side effects on the human mucosa, while the manufacturing process has a complex disadvantage.

An object of the present invention created to solve the above problems is to provide an anti-bacterial anti-inflammatory sea lacto cosmetic composition using marine-derived natural substances and lactic acid bacteria and a method for manufacturing the same.

The object of the present invention is, (a) preparing a processed Ecklonia cava extract, a hetspberry extract, a kelp extract, a radiated patterned rock extract, a brown algae extract, a lactic acid bacterium and an oligosaccharide; (b) Based on 100 parts by weight of Ecklonia cava extract, 5 to 10 parts by weight of Totilla extract, 20 to 35 parts by weight of kelp extract, 5 to 8 parts by weight of radish-patterned seaweed extract, 5 to 10 parts by weight of brown algae extract, 30 to 90 parts by weight of lactic acid bacteria Mixing parts and 3 to 25 parts by weight of oligosaccharide and stirring for 30 to 45 minutes; (c) fermenting at 30 to 43° C. for 3 to 6 months and then filtering to prepare a crude filtrate; (d) a method for preparing an anti-bacterial anti-inflammatory shellacto cosmetic composition comprising the step of preparing an anti-bacterial anti-inflammatory shellacto cosmetic composition by adding 3 to 50 parts by weight of fucose to 100 parts by weight of the crude filtrate and stirring for 10 to 12 hours Can be achieved by

Preferably, the Ecklonia cava extract of the step (a) of the present invention is 90 to 130 parts by weight of dried Ecklonia cava and 0.1 to 0.3 parts by weight of dried Ecklonia cava based on 100 parts by weight of purified water. It is prepared by heating at °C for 12 to 15 hours and then filtering, and the extract of the hepatica extract of step (a) is 50 to 55 parts by weight of hemp powder, 0.1 to 0.1 parts by weight of grapefruit seed extract and olive oil 1 based on 100 parts by weight of purified water. Prepared by filling ~ 1.5 parts by weight in an airtight container, heating at 90 to 130°C for 12 to 15 hours, and then filtering. The kelp extract of step (a) is prepared by (a) dried kelp in purified water for 5 to 10 days. Re-drying at 10 to 15° C. after immersion; (B) repeating the process of drying the re-dried kelp after self-cooking 1 to 5 times; (C) pulverizing the self-dried kelp to form a powder; With respect to 100 parts by weight of purified water, 5 to 15 parts by weight of kelp powder is mixed, stirred for 30 to 35 days at a rotational speed of 100 to 150 rpm, filtered, and then stored at 10 to 15°C for 120 to 150 days. , After filling the radiated pattern protrusion extract of step (a) into an airtight container, 30 to 35 parts by weight of radioactive pattern protrusion powder, 10 to 15 parts by weight of soybean curd powder, and 1 to 1.5 parts by weight of olive oil based on 100 parts by weight of purified water It is characterized in that it is prepared by heating at 90 to 130°C for 5 to 7 hours and then filtering.

Preferably, after the step (d) of the present invention, further comprising a step (e) of further adding 1 to 50 parts by weight of seaweed fermentation extract based on 100 parts by weight of the antibacterial anti-inflammatory sealacto cosmetic composition, wherein the seaweed fermented extract Silver, (a) based on 100 parts by weight of purified water, 10 to 15 parts by weight of dry chopped Ecklonia cava, 5 to 7 parts by weight of dry chopped soybean paste, 5 to 10 parts by weight of dry chopped kelp, 5 to 10 parts by weight of spinneret And 3 to 5 parts by weight of finely dried brown algae, followed by heating at 120 to 150° C. for 120 to 150 minutes, cooling to 30 to 35° C., and filtering to prepare a filtrate; (B) Based on 100 parts by weight of the filtrate, 80 to 130 parts by weight of sugar and 1 to 3 parts by weight of grapefruit seed extract are filled in an airtight container, fermented at 30 to 35°C for 3 to 5 months, and then filtered. It characterized in that it consists of steps.

More preferably, the lactic acid bacteria is one or more of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium longum, Bifidobacterium bifidum, Actiregularis or Lactobacillus rhamnosus. It is characterized by that.

In addition, the present invention can be achieved by the anti-bacterial anti-inflammatory siracto cosmetic composition prepared by any one of the above methods.

The present invention as described above has an effect of mass-producing a cosmetic composition capable of alleviating skin troubles with excellent antibacterial and anti-inflammatory effects using natural materials and lactic acid bacteria derived from the ocean. In particular, the present invention has an advantage that can be applied to a feminine cleaner with good safety in use so that girls and women can use it together.

Hereinafter, it will be described with reference to an embodiment of the present invention.

1. Making seaweed extract

Ecklonia cava extract (Ecklonia cava extract)

Use high-pressure water or high-pressure seawater to remove foreign substances from Ecklonia and dry naturally.

Thereafter, the dried Ecklonia cava is immersed in purified water for 30 to 120 minutes, and then re-dried after ultrasonic cleaning. Here, the drying and ultrasonic cleaning of Ecklonia cava is to remove the salt remaining in Ecklonia cava, a natural product derived from the ocean as much as possible.

Subsequently, with respect to 100 parts by weight of purified water, 30 to 45 parts by weight of dried Ecklonia cava and 0.1 to 0.3 parts by weight of Sancho Seed oil are filled in an airtight container, heated at 90 to 130° C. for 12 to 15 hours, and filtered to prepare. Here, the sanchoseed oil has pain relief, antioxidant and anti-cancer effects, and is provided to capture rancidity of the extract and the odor of ecstasy.

In general, Ecklonia cava is a perennial plant distributed in Jeju Island and some southern coasts of Korea, and is a health food resource containing a lot of nutrients such as alginic acid, iodine and potassium. In addition, Ecklonia cava contains fucoidan and fluorotannin components, so it has an antioxidant effect, an anticancer effect, an anti-inflammatory effect, an anti-aging effect, and an antihypertensive effect.

Chop extract (Hizikia Fusiforme extract)

Use high-pressure water or high-pressure seawater to remove foreign substances from Tot and then dry naturally.

Thereafter, the dried tot is passed through a rolling roller and then made into powder. Here, the reason why the dried tot is made into powder after rolling is to smoothly implement the extraction process described later.

Subsequently, 50 to 55 parts by weight of hepatitis powder, 0.1 to 0.1 parts by weight of grapefruit seed extract, and 1 to 1.5 parts by weight of olive oil were filled in an airtight container and heated at 90 to 130°C for 12 to 15 hours, based on 100 parts by weight of purified water. Then prepare by filtering. Here, grapefruit seed extract is a natural preservative, and olive oil is an antifoam.

In general, tot is a perennial algae that grows widely from south of Jumunjin in Korea to Jangsangot on the west coast. It contains a lot of inorganic salts such as calcium, iodine, and iron, so it has the effect of preventing vascular sclerosis. It is rich in fiber, so it has constipation and digestive function of the intestines. It is an effective health food resource.

Seaweed extract (Laminaria Japonica extract)

Use high-pressure water or high-pressure seawater to remove foreign substances from the kelp and dry.

Thereafter, the dried kelp is immersed in purified water for 5 to 10 days and then naturally dried at 10 to 15°C so that the moisture content is less than 3%. Here, the reason why the dried kelp is immersed in purified water is to remove salt.

Then, after the dried kelp is boiled, the process of drying is repeated 1 to 5 times. Here, the reason for repeating the self-cooking and drying process is to concentrate fucoidan contained in kelp.

Subsequently, the dried kelp is pulverized to form a powder.

Finally, 5 to 15 parts by weight of kelp powder is mixed with respect to 100 parts by weight of purified water, and then stirred for 30 to 35 days at a rotational speed of 100 to 150 rpm, filtered, and then stored at 10 to 15°C for 120 to 150 days. Here, the reason for using the non-heating method of the mixture of purified water and kelp powder is to prevent the fucoidan and laminin, which are active ingredients, from being damaged.

Kelp is a large sea horse that lives on the Pacific coast of Korea, Japan, Kamchatka Peninsula, and Sakhalin Island. It is born in 2 to 4 years, has a lot of inorganic salts such as iodine, potassium, and calcium, and is a health food resource with a lot of laminin and fucoidan.

Radiation pattern Seaweed extract (Porphyra Yezoensis extract)

Use high-pressure water or high-pressure seawater to remove foreign substances from the radiating pattern, and dry them naturally, then pulverize to make powder.

Subsequently, with respect to 100 parts by weight of purified water, 30 to 35 parts by weight of radiated pattern powder, 10 to 15 parts by weight of bean curd powder, and 1 to 1.5 parts by weight of olive oil are filled in an airtight container, and then heated at 90 to 130°C for 5 to 7 hours. And then filter to prepare. Here, olive oil is an antifoaming agent, and soybean curd powder is a natural precipitant provided to trap and precipitate the remaining salts of the radioactive pattern protrusions.

Radiation-patterned laver grows from the intertidal zone to the upper part of the lunch table along the coastline of Korea and Japan, and is a sea horse of the red algae plant purple hair that grows well in high salinity places facing the open sea. It is also late and has the effect of imparting conditioning to the skin and hair.

Algae extract

Brown algae of this embodiment is revealed in advance that including Ecklonia cava, Tot, seaweed excluding kelp, great disappointment, crucian carp, edamame, rhubarb, and half hatch.

Brown algae extract is prepared by swelling brown algae with sodium chloride solution, forming a colloid, centrifuging, and filtering.

2. Manufacture fermented seaweed extract

With respect to 100 parts by weight of purified water, 10 to 15 parts by weight of dried chopped Ecklonia cava, 5 to 7 parts by weight of dried chopped kombu, 5 to 10 parts by weight of dried chopped kelp, 5 to 10 parts by weight of radiated pattern protrusions, and shredded brown algae After mixing 3 to 5 parts by weight, heating at 120 to 150°C for 120 to 150 minutes, cooling to 30 to 35°C, and filtering to prepare a filtrate.

Thereafter, based on 100 parts by weight of the filtrate, 80 to 130 parts by weight of sugar and 1 to 3 parts by weight of grapefruit seed extract are filled in an airtight container, followed by fermentation at a temperature of 30 to 35° C. for 3 to 5 months, followed by filtration.

3. Manufacture of antibacterial and anti-inflammatory sealacto cosmetic composition

(a) Prepare the Ecklonia cava extract, hepatitis extract, kelp extract, radish-patterned laver extract, brown algae extract, fermented seaweed extract, lactic acid bacteria and oligosaccharides.

Here, the lactic acid bacteria used in this practice mean probiotics including Lactobacillus and Bifidobacterium, and L. casei, L. acidophilus, and Lactobacillus bulgaricus (L.bulgaricus), Bifidobacterium longum, B.bifidum, Actiregularis, L.rhamnosus, and various other types. Lactobacillus of may be present.

In addition, oligosaccharides, which are food for lactic acid bacteria, are sugars that contain 2 to 8 sugars such as glucose, fructose, and galactose, and are water-soluble, crystalline substances with sweet taste. , It is a substance composed of sugar made by enzyme synthesis, and it is a material derived from carbohydrates, and has excellent physiological functions and physicochemical properties, and has a wide range of application to food.

(b) Based on 100 parts by weight of Ecklonia cava extract, 5 to 10 parts by weight of Totilla extract, 20 to 35 parts by weight of kelp extract, 5 to 8 parts by weight of radish-patterned seaweed extract, 5 to 10 parts by weight of brown algae extract, 30 to 90 parts by weight of lactic acid bacteria Parts and 3 to 25 parts by weight of oligosaccharide are mixed and stirred for 30 to 45 minutes.

(c) Then, it is fermented at 30 to 43°C for 3 to 6 months and then filtered to prepare a crude filtrate.

(d) Thereafter, 3 to 50 parts by weight of fucose is further added to 100 parts by weight of the crude filtrate and then stirred for 10 to 12 hours to prepare a final anti-bacterial anti-inflammatory shellacto cosmetic composition. Here, fucose is a kind of 6-deoxysaccharide, and the D- and L-forms are non-reducing end sugars of various sugars and lipids, and are also called 6-deoxygalactose, galactomethylose, and rhodeose. Since it is a known sugar component, a detailed description thereof will be omitted.

In addition, it may further include a step (e) of further adding 1 to 50 parts by weight of seaweed fermented extract based on 100 parts by weight of the anti-bacterial anti-inflammatory sealacto cosmetic composition. Here, the seaweed fermented extract is used as a natural preservative.

4. Evaluation test for inhibition of NO (Nitric Oxide) production of antibacterial anti-inflammatory sea lacto cosmetic composition

-Purpose of experiment: Confirming the anti-inflammatory activity of the sample by conducting the NO content test of the anti-bacterial anti-inflammatory seed lacto cosmetic composition.

-Laboratory: Chungcheongbuk-do Cosmetics Clinical Research Support Center

-Experimental cell line: Raw246.7

-Laboratory equipment and reagents

Autoclave(Coretech, Korea), Dry oven(DAIHAN, Korea), Water bath(Polyscience, USA), Incubator(DAIHAN, Korea), pH meter(Thermo Orion, USA), Vortex mixer(DAIHAN, Korea), Container ( Iwaki Pyrex, Japan), Sterile pipette (Eppendorf, Germany), Volumetric flask (KYUNG IN, Korea), Mechanical shaker (Jisico, Korea), Clean bench (CHC, Korea), DMSO (Sigma, USA), DMEM high glucose ( Biowest, USA), Fetal Bovine Serum (Biowest, USA), Antibiotics (Gibco, USA), MTT (Invitrogen, USA), LPS (Sima, USA), NO diagnostic kit (Griess Reagent System, USA)

Experiment method

The group configuration is shown in Table 1 below.

㉯ cell dispensing

The cells were dispensed in a 96 well plate at a ^{concentration of 2 × 10 5} cells/well, and cultured for 24 hours in a 37°C, 5% CO _{2 incubator.}

㉰ Test substance treatment

With the medium to which LPS was added, the test substance was replaced with a medium diluted by concentration, and cultured in a 37°C, 5% CO ₂ incubator for 24 hours.

㉱ Measurement of NO generation inhibition

50 µg/mL of cell culture solution and 50 µg/mL of Griess reagent were mixed and left in the dark, and the absorbance was measured at a wavelength of 520 nm.

㉲ result processing

As for the amount of NO produced, the NO content in the culture medium was calculated by preparing a standard curve for each concentration using the Nitric Oxide standard solution.

Experiment result

When treated with a 20-fold dilution concentration using C. lacto complex as a stock solution, a 47.79% inhibition rate of NO production was observed compared to the LPS treatment group.

As a result, in this test, the inhibitory effect of NO (Nitric Oxide) production was measured in order to evaluate the anti-inflammatory activity of the C-lactocomplex sample. Based on the cytotoxicity results, when the C-lactocomplex sample was treated with a stock solution at a 20-fold dilution concentration, a 47.79% inhibition rate of NO production was observed compared to the LPS-treated group, confirming that the 20-fold dilution concentration showed an inhibitory effect on NO production. I did.

The present invention has been described by being limited to one embodiment, but the embodiments are not limited thereto, and all embodiments modified based on the technical idea of the present invention belong to the scope of the present invention.

Claims (5)

(a) preparing the processed Ecklonia cava extract, hepatitis extract, kelp extract, radiated pebbles extract, brown algae extract, lactic acid bacteria and oligosaccharides;
(b) Based on 100 parts by weight of Ecklonia cava extract, 5 to 10 parts by weight of Toot extract, 20 to 35 parts by weight of kelp extract, 5 to 8 parts by weight of radish-patterned seaweed extract, 5 to 10 parts by weight of brown algae extract, 30 to 90 parts by weight of lactic acid bacteria Mixing parts and 3 to 25 parts by weight of oligosaccharide and stirring for 30 to 45 minutes;
(c) fermenting at 30 to 43° C. for 3 to 6 months and then filtering to prepare a crude filtrate;
(d) a method for preparing an anti-bacterial anti-inflammatory shellacto cosmetic composition comprising the step of preparing an anti-bacterial anti-inflammatory shellacto cosmetic composition by adding 3 to 50 parts by weight of fucose to 100 parts by weight of the crude filtrate and then stirring for 10 to 12 hours .
The method of claim 1,
The Ecklonia cava extract of step (a) is heated at 90 to 130°C for 12 to 15 hours after filling 30 to 45 parts by weight of dried Ecklonia and 0.1 to 0.3 parts by weight of Sancho Seed oil in an airtight container based on 100 parts by weight of purified water. And then filtered to prepare,
In the above step (a), based on 100 parts by weight of purified water, 50 to 55 parts by weight of Hemischia powder, 0.1 to 0.1 parts by weight of grapefruit seed extract, and 1 to 1.5 parts by weight of olive oil are filled into an airtight container, and then 90 to 130°C. It is prepared by heating at 12 to 15 hours and then filtering,
The kelp extract of step (a) includes the steps of: (a) immersing the dried kelp in purified water for 5 to 10 days and then redrying at 10 to 15°C; (B) repeating the process of drying the re-dried kelp after self-cooking 1 to 5 times; (C) pulverizing the self-dried kelp to form a powder; With respect to 100 parts by weight of purified water, 5 to 15 parts by weight of kelp powder is mixed, stirred at a rotational speed of 100 to 150 rpm for 30 to 35 days, filtered, and then stored at 10 to 15°C for 120 to 150 days. ,
The radiated pattern protrusion extract of step (a) is charged in an airtight container with 30 to 35 parts by weight of radiant pattern protrusion powder, 10 to 15 parts by weight of bean curd powder, and 1 to 1.5 parts by weight of olive oil based on 100 parts by weight of purified water. Method for producing an anti-bacterial anti-inflammatory sea lacto cosmetic composition, characterized in that prepared by heating at ~ 130 ℃ for 5 to 7 hours and then filtered.
The method of claim 1,
After the step (d), further comprising a step (e) of further adding 1 to 50 parts by weight of seaweed fermented extract with respect to 100 parts by weight of the antibacterial anti-inflammatory sea lacto cosmetic composition,
The fermented seaweed extract is, (a) 10 to 15 parts by weight of dried chopped Ecklonia cava, 5 to 7 parts by weight of dried chopped kombu, 5 to 10 parts by weight of dried chopped kelp, and 5 Mixing ~ 10 parts by weight and 3 to 5 parts by weight of finely dried brown algae, heating at 120 to 150°C for 120 to 150 minutes, cooling to 30 to 35°C, and filtering to prepare a filtrate; (B) Based on 100 parts by weight of the filtrate, 80 to 130 parts by weight of sugar and 1 to 3 parts by weight of grapefruit seed extract are filled in an airtight container, fermented at 30 to 35°C for 3 to 5 months, and then filtered. Method for producing an antibacterial anti-inflammatory sea lacto cosmetic composition, characterized in that consisting of steps.
The method of claim 1,
The lactic acid bacteria are antibacterial, characterized in that at least one of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium longum, Bifidobacterium bifidum, Actiregularis or Lactobacillus rhamnosus Method for producing an anti-inflammatory sea lacto cosmetic composition.
Antibacterial anti-inflammatory sea lacto cosmetic composition prepared by any one of claims 1 to 4.