CN110256598A - A kind of preparation method of difference bioactivity lentinan - Google Patents
A kind of preparation method of difference bioactivity lentinan Download PDFInfo
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- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
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Abstract
The present invention provides a kind of preparation method of different bioactivity lentinans, is related to biological polyoses technical field, and the present invention crosses 60-80 mesh screen, obtain mushroom powder the following steps are included: (1) crushing after dry, pulverize mushroom fruiting body；(2) extract, take mushroom powder that pure water is added and obtain mixed liquor, the mushroom powder, pure water ratio be 1:20-40 m/v, heating mixed liquor is extracted at 90-100 DEG C；(3) it filters, the first leaching liquor is obtained after filtering, filter residue carries out secondary extraction, obtains extraction filtrate；(4) it is classified alcohol precipitation, extraction filtrate obtains concentrate after being concentrated under reduced pressure, carries out classification alcohol precipitation, obtain the polysaccharide precipitation of a variety of different component contents；(5) polysaccharide is dry, respectively dries polysaccharide precipitation, obtains a variety of lentinans.The present invention can obtain the lentinan that different bioactivity, polyoses content are different, molecular weight is different, and the yield of polysaccharide is high, sufficiently promote the application value of lentinan, be conducive to the classification application of lentinan.
Description
Technical field
The present invention relates to biological polyoses technical fields, and in particular to a kind of preparation side of difference bioactivity lentinan
Method.
Background technique
Past 25 years, new natural drug was approved for treatment human diseases, in the R&D process of new drug,
Natural products plays a highly important role, and for hundreds of years, Shitake Mushroom P.E is taken as drug use, or even in
State, Japan, South Korea and Russian east are as substitution medicine.Plurality of active ingredients in mushroom is it is verified that be able to suppress cancer cell
Formation, wherein the studied polysaccharose substance preferably at most with enhancing host immune response, modern clinic experiments have shown that, it is fragrant
Containing there are many effective medicinal ingredient in mushroom, especially wherein lentinan is able to suppress cancer cell, hypoglycemic, anti-oxidant, resists
Tumour and preventing cold, improve the immunity of the human body.
Mushroom mainly carries out large-scale plantation using generation material or section wood at present, under suitable management condition, mushroom one
As will appear 4 damp mushrooms, the growth and development stage of each tide mushroom is generally divided into mushroom flower bud phase, immature phase fructification, and maturity period is real
Four periods of body and parachute-opening phase fructification.Polyoses producing method traditional at present all uses the mushroom fruiting body in maturity period, and
Alcohol precipitation is carried out under the conditions of 70% or more ethyl alcohol final concentration obtains lentinan, excessively high concentration of alcohol is the structure for destroying polysaccharide,
The lentinan for influencing its medical value, and utilizing is mostly to decolourize, take off proteoglycan.
A kind of isolation and purification method of lentinan is disclosed in the patent of Publication No. CN1153179, the patent
The yield of middle lentinan very low about 0.1%, causes great mushroom to waste in this way, increases the production cost of lentinan.
Due to the loss of Medium Culture nutritional ingredient, the variation of growing environment and human factor etc. one during mushroom growth
Serial reason causes different growth and development stage lentinan qualities certain otherness occur, meanwhile, it is dense eventually using different ethyl alcohol
The lentinan activity that degree obtains also has certain otherness.Lentinan is extracted in the prior art uses maturity period mushroom mostly
Fructification, the disadvantage is that without generated biological polyoses in the entire growth course of reasonable utilization mushroom, because different
The activity of the lentinan contained in growth period mushroom is different, and targeted medical care effect also has more difference, only
Extracting the lentinan in the mushroom fruiting body in maturity period will limit the diversity of lentinan, not make full use of the doctor of mushroom
Use health value.
Production technology in the prior art often purifies lentinan to obtain single polysaccharide, or is taken off
Color, the processing of de- albumen, the inoxidizability of obtained product, health and bad.
Summary of the invention
In view of this, the present invention provides a kind of preparation method of different bioactivity lentinans, the concentration of alcohol used
It is low, using the mushroom fruiting body of different growing stages as raw material, different bioactivity, polyoses content difference, molecular weight can be obtained not
The yield of same lentinan, polysaccharide is high, sufficiently promotes the application value of lentinan, while effectively reducing lentinan
Preparation cost.
The present invention is a kind of preparation method of different bioactivity lentinans, comprising the following steps:
(1) it crushing, mushroom fruiting body is dry, and dry temperature is 40-60 DEG C, and the dry time is 6-12h,
60-80 mesh screen is smashed it through, mushroom powder is obtained, mushroom fruiting body is the fructification of mushroom flower bud phase, the son of immature phase
One of entity, the fructification in maturity period, fructification of parachute-opening phase are a variety of；
Raw material uses the mushroom fruiting body of different times, and the fructification of mushroom flower bud phase, fructification is undifferentiated, and bacteria cover diameter is about 0.5
cm-1.0 cm；The velum of immature phase fructification is formed, and bacteria cover diameter is about 1.5 cm-2.0 cm)；Maturity period, (velum was opened
Split) and the parachute-opening phase (velum splits completely, the complete parachute-opening of fructification).The lentinan of the mushroom fruiting body of different growth and development stages
Having a certain difference property of quality can also can be selected according to the type for needing to select mushroom fruiting body to polysaccharide different activities
The form of the mushroom fruiting body mixing of a variety of growth cycles is produced as raw material and has multiple biological activities lentinan.It is fragrant
The particle size at mushroom powder end will affect the effect of extraction, lentinan be the effective active that is extracted from high quality mushroom fruiting body at
Point, the active constituent in lentinan is β-(the 1-3)-D- glucan with branch, main chain by β-(1-3)-connection grape
Glycosyl composition, the glucosyl group connected along main chain random distribution by β-(1-6) is in pectinate texture, and lentinan is mostly acid
Property polysaccharide, be dissolved in water, diluted alkaline, be especially soluble in hot water, so lentinan is dissolved into hot water when extraction, refinement is fragrant
Mushroom powder end is conducive to improve its leaching effect.
Dry temperature is lower at 40-60 DEG C, because excessively high temperature can change the structure or chemical property of polysaccharide,
It is dry in lower temperature range, guarantee the activity of lentinan to greatest extent.
(2) it extracts, takes mushroom powder that pure water is added and obtain mixed liquor, the ratio of the mushroom powder, pure water
It for 1:(20-40) m/v, heats mixed liquor and is extracted for the first time at 90-100 DEG C, the time of extraction is 2-3h；
Lentinan is soluble in hot water, and lentinan can be dissolved out gradually in 90-100 DEG C, because to perfume (or spice) before extraction
Mushroom carries out dispersion and fining, the extracting effect in leaching process can be improved, in addition suitable extraction temperature, it is more to effectively improve mushroom
The yield of sugar.
(3) it filtering, the first leaching liquor is obtained after filtering, filter residue carries out secondary extraction, obtains the second leaching liquor,
Merge the first leaching liquor, the second leaching liquor, obtains extraction filtrate；；
The effect of extraction can be improved, finally can be improved yield as needed to filter residue extracted many times.
(4) it is classified alcohol precipitation, extraction filtrate obtains concentrate after being concentrated under reduced pressure, and the cocnentration factor of reduced pressure is
3-5:1 v/m, cocnentration factor are extraction filtrate volume: mushroom powder quality.Ethyl alcohol is added several times, successively makes final
Concentration of alcohol is 20-70% mL/mL, carries out classification alcohol precipitation, obtains the polysaccharide precipitation of a variety of different component contents, such as specific behaviour
Making can be with are as follows: ethyl alcohol is added, makes 20% mL/mL of the concentration progress alcohol precipitation of ethyl alcohol in mixed liquor first, precipitate A is collected by centrifugation；
Then it taking and was centrifuged remaining supernatant just now, ethyl alcohol is added into supernatant makes 50% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation,
Precipitate B is collected by centrifugation；Continuing up addition ethyl alcohol in clear liquid makes 70% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, is collected by centrifugation
Precipitate C obtains three kinds of alcohol precipitation polysaccharide precipitations, composition difference, the bioactivity of A polysaccharide precipitation, B polysaccharide precipitation, C polysaccharide precipitation
It is not identical.The ethyl alcohol that a kind of concentration can only be added according to specific needs carries out the extraction of lentinan.
(5) polysaccharide is dry, respectively dries a variety of polysaccharide precipitations, obtains a variety of lentinans.
The revolving speed of centrifugation is 4000-5000 r/min, and centrifugation time is 15-20 min, and the temperature of alcohol precipitation is 4-5 DEG C,
The time of alcohol precipitation is 6-12h.
Reasonable cocnentration factor, the concentration of alcohol of alcohol precipitation, the temperature of alcohol precipitation, alcohol precipitation time mushroom can be improved
The yield and quality of polysaccharide.
(5) polysaccharide is dry, respectively dries a variety of polysaccharide precipitations, obtains a variety of lentinans.The temperature of drying is
45-58℃。
It is not decolourized in the preparation process of lentinan of the invention, de- albumen processing, does not carry out the column point of polysaccharide
From purifying, obtained product lentinan is the Thick many candies for being classified alcohol precipitation.Our early-stage studies the result shows that the decoloration of optimization and
For the lentinan loss late of de- protein Process 9% or so, the column separating purification process of polysaccharide can equally lose par-tial polysaccharide,
For this purpose, the lentinan of this patent preparation can guarantee higher polysaccharide yield and polyoses content；In addition our research discovery
Lentinan sample before purification shows better antioxidant activity, illustrate wherein protein or other active material with
The synergistic effect of polysaccharide makes it have better oxidation resistance, for this purpose, being not purer for the effective use of lentinan
It is good, the lentinan of health care product or food additives is done, using Thick many candies, alimentary health-care function is more excellent.
Using different ethanol concentration, using the mushroom fruiting body of different growing stages as raw material, different bioactivity can be obtained
Polysaccharide, the yield of polysaccharide is high, sufficiently promotes the application value of lentinan, identical mushroom leaching liquor can propose more simultaneously
Kind polysaccharide precipitation can improve the recovery rate to polysaccharide in raw materials of letinous edodes with the simplification production technology of high degree, it is former to save mushroom
The usage amount of material.
Specific embodiment
The present invention will be described in detail With reference to embodiment.
Embodiment 1
The present invention is a kind of preparation method of different bioactivity lentinans, comprising the following steps:
(1) it crushes, mushroom fruiting body is dry, and dry temperature is 40 DEG C, and the dry time is 12h, smashes it through 60 meshes
Net, obtains mushroom powder, and mushroom fruiting body is the fructification of mushroom flower bud phase；
(2) extract, take mushroom powder that pure water is added and obtain mixed liquor, the mushroom powder, pure water ratio be 1:40 m/v, heating
Mixed liquor extracts for the first time at 90 DEG C, and the time of extraction is 2h；
(3) it filters, the first leaching liquor is obtained after filtering, filter residue carries out second and extracts, and obtains the second leaching liquor, merges the first leaching
Extraction filtrate is obtained after extract, the second leaching liquor；
(4) alcohol precipitation, extraction filtrate obtain concentrate after being concentrated under reduced pressure, and the cocnentration factor of reduced pressure is 3:1,
Cocnentration factor be extraction filtrate volume: mushroom powder quality, be added ethyl alcohol, make first 20% mL/mL of concentration of ethyl alcohol into
Row alcohol precipitation, is collected by centrifugation precipitate A；Then proceeding to the addition ethyl alcohol into supernatant makes 50% mL/mL of concentration of ethyl alcohol carry out alcohol
It is heavy, precipitate B is collected by centrifugation；Continuing up addition ethyl alcohol in clear liquid makes 70% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, and centrifugation is received
Collect precipitate C, the revolving speed of centrifugation is 4000r/min, and the temperature of centrifugation time 15min, alcohol precipitation are 4 DEG C, and the time of alcohol precipitation is
12h obtains three kinds of different component polysaccharide precipitations；
(5) polysaccharide is dry, will obtain three kinds of lentinans after the polysaccharide precipitation drying of three kinds of different components respectively, A polysaccharide, B are more
Sugar, C polysaccharide, the temperature of drying are 58 DEG C.
After tested, A polysaccharide in the present embodiment, B polysaccharide, C polysaccharide yield be respectively 10.99%, 6.89%, 1.18%, it is total
Polysaccharide yield is 19.06%；A polysaccharide, B polysaccharide, polyoses content is respectively 42.52%, 61.18%, 47.18% in C polysaccharide.
Wherein the beta glucan content in 20% concentration of alcohol alcohol precipitation polysaccharide (A polysaccharide) component is 15.20%, protein content
It is 10.95%, viscosity average molecular weigh is 150.18 KDa, and the yield that stimulated in vitro macrophage discharges NO is 58.41 μm of ol/mL,
It is in vitro 48.31%, DPPH Scavenging activity to the inhibiting rate of liver cancer cells is 49.32%, Hydroxyl radical-scavenging ability is 14.73%,
Superoxide ion Scavenging activity is 4.21%；
Wherein the beta glucan content in 50% concentration of alcohol alcohol precipitation polysaccharide (B polysaccharide) component is 30.14%, protein content is
8.56%, viscosity average molecular weigh is 82.06 KDa, and the yield that stimulated in vitro macrophage discharges NO is 40.93 μm of ol/mL, external right
The inhibiting rate of liver cancer cells is that 29.09%, DPPH Scavenging activity is 49.32%, Hydroxyl radical-scavenging ability be 5.90%, super oxygen from
Sub- Scavenging activity is 5.32%；
Wherein the beta glucan content in polysaccharide (C polysaccharide) component of 70% concentration of alcohol alcohol precipitation is 33.25%, protein content is
8.65%, viscosity average molecular weigh is 82.06 KDa, and the yield that stimulated in vitro macrophage discharges NO is 39.32 μm of ol/mL, in vitro
Inhibiting rate to liver cancer cells is that 27.15%, DPPH Scavenging activity is 34.92%, and Hydroxyl radical-scavenging ability is 13.28%, super oxygen
Ion Scavenging activity is 7.45%.
Embodiment 2
The present invention is a kind of preparation method of different bioactivity lentinans, comprising the following steps:
(1) it crushes, mushroom fruiting body is dry, and dry temperature is 60 DEG C, and the dry time is 6h, with 80 mesh screens after crushing
Filtering, obtains mushroom powder, and mushroom fruiting body is the fructification of immature phase；
(2) extract, take mushroom powder that pure water is added and obtain mixed liquor, the mushroom powder, pure water ratio be 1:20 m/v, add
Hot mixing liquid extracts for the first time at 100 DEG C, and the time of extraction is 3h；
(3) it filters, the first leaching liquor is obtained after filtering, filter residue carries out second and extracts, and obtains the second leaching liquor, merges the first leaching
Extraction filtrate is obtained after extract, the second leaching liquor；
(4) it is classified alcohol precipitation, extraction filtrate obtains concentrate after being concentrated under reduced pressure, and the cocnentration factor of reduced pressure is 5:1, and cocnentration factor is
Filtrate volume: mushroom powder quality is extracted, ethyl alcohol is added, 20% mL/mL of concentration of ethyl alcohol is made to carry out alcohol precipitation first, centrifugation is received
Collect precipitate A, then proceeding to the addition ethyl alcohol into supernatant makes 50% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, and it is heavy to be collected by centrifugation
Shallow lake B, continuing up addition ethyl alcohol in clear liquid makes 70% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, and precipitate C is collected by centrifugation, is centrifuged
Revolving speed be 5000 r/min, centrifugation time is 20 min, and the temperature of alcohol precipitation is 5 DEG C, and the time of alcohol precipitation is 6h, obtains three kinds not
It is precipitated with fraction polysaccharide；
(5) polysaccharide is dry, will obtain three kinds of lentinans after the polysaccharide precipitation drying of three kinds of different components respectively, A polysaccharide, B are more
Sugar, C polysaccharide, the temperature of drying are 45 DEG C.
After tested, the present embodiment is using 20%, 50%, 70% concentration of alcohol classification alcohol precipitation three kinds of lentinan components of gained
Yield is respectively 14.60%, 3.93%, 1.35%, and total polysaccharide yield is 19.88%；A polysaccharide, B polysaccharide, polyoses content in C polysaccharide
Respectively 32.77%, 62.70% 55.95%.
Wherein the beta glucan content in 20% concentration of alcohol alcohol precipitation polysaccharide component is 13.66%, protein content is
12.83%, viscosity average molecular weigh is 219.22 KDa, and the yield that stimulated in vitro macrophage discharges NO is 70.21 μm of ol/mL, body
It is outside 46.29%, DPPH Scavenging activity to the inhibiting rate of liver cancer cells is 57.38%, Hydroxyl radical-scavenging ability is 24.01%, is surpassed
Oxonium ion Scavenging activity is 9.70%.
Embodiment 3
The present invention is a kind of preparation method of different bioactivity lentinans, comprising the following steps:
(1) it crushes, mushroom fruiting body is dry, and dry temperature is 55 DEG C, and the dry time is 10h, smashes it through 70 meshes
Net, obtains mushroom powder, and mushroom fruiting body is the fructification in maturity period；
(2) extract, take mushroom powder that pure water is added and obtain mixed liquor, the mushroom powder, pure water ratio be 1:30 m/v, heating
Mixed liquor extracts for the first time at 95 DEG C, and the time of extraction is 2.5h；
(3) it filters, the first leaching liquor is obtained after filtering, filter residue carries out second and extracts, and obtains the second leaching liquor, merges the first leaching
Extraction filtrate is obtained after extract, the second leaching liquor；
(4) it is classified alcohol precipitation, extraction filtrate obtains concentrate after being concentrated under reduced pressure, and the cocnentration factor of reduced pressure is 4:1, and cocnentration factor is
Filtrate volume: mushroom powder quality is extracted, ethyl alcohol is added, 25% mL/mL of concentration of ethyl alcohol is made to carry out alcohol precipitation first, centrifugation is received
Collect precipitate A, then proceeding to the addition ethyl alcohol into supernatant makes 45% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, and it is heavy to be collected by centrifugation
Shallow lake B, continuing up addition ethyl alcohol in clear liquid makes 60% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, and precipitate C is collected by centrifugation, is centrifuged
Revolving speed be 4500 r/min, centrifugation time is 16 min, and the temperature of alcohol precipitation is 4.5 DEG C, and the time of alcohol precipitation is 10h, obtains three
Kind different component polysaccharide precipitation；
(5) polysaccharide is dry, will obtain three kinds of lentinans after the polysaccharide precipitation drying of three kinds of different components respectively, A polysaccharide, B are more
Sugar, C polysaccharide, the temperature of drying are 50 DEG C.
After tested, the present embodiment is using 25%, 45%, 60% concentration of alcohol classification alcohol precipitation three kinds of lentinan components of gained
Yield is respectively 10.08%, 1.50%, 1.78%, and total polysaccharide yield is 13.36%；A polysaccharide, B polysaccharide, polyoses content in C polysaccharide
Respectively 29.49%, 70.29%, 60.99%.
Wherein the beta glucan content in 45% concentration of alcohol alcohol precipitation polysaccharide component is 31.74%, protein content is
9.34%, viscosity average molecular weigh is 84.85 KDa, and the yield that stimulated in vitro macrophage discharges NO is 41.29 μm of ol/mL, in vitro
Inhibiting rate to liver cancer cells is that 28.67%, DPPH Scavenging activity is 13.25%, and Hydroxyl radical-scavenging ability is 12.55%, super oxygen
Ion Scavenging activity is 6.21%.
Embodiment 4
The present invention is a kind of preparation method of different bioactivity lentinans, comprising the following steps:
(1) it crushes, mushroom fruiting body is dry, and dry temperature is 60 DEG C, and the dry time is 12h, smashes it through 60 meshes
Net, obtains mushroom powder, and mushroom fruiting body is the fructification of parachute-opening phase；
(2) extract, take mushroom powder that pure water is added and obtain mixed liquor, the mushroom powder, pure water ratio be 1:25 m/v, heating
Mixed liquor extracts for the first time at 95 DEG C, and the time of extraction is 2h；
(3) it filters, the first leaching liquor is obtained after filtering, filter residue carries out second and extracts, and obtains the second leaching liquor, merges the first leaching
Extraction filtrate is obtained after extract, the second leaching liquor；
(4) it is classified alcohol precipitation, extraction filtrate obtains concentrate after being concentrated under reduced pressure, and the cocnentration factor of reduced pressure is 3:1, and cocnentration factor is
Filtrate volume: mushroom powder quality is extracted, ethyl alcohol is added, 30% mL/mL of concentration of ethyl alcohol is made to carry out alcohol precipitation first, centrifugation is received
Collection precipitating, then proceeding to the addition ethyl alcohol into supernatant makes 55% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, and it is heavy to be collected by centrifugation
It forms sediment, continuing up addition ethyl alcohol in clear liquid makes 70% mL/mL of concentration of ethyl alcohol carry out alcohol precipitation, precipitating is collected by centrifugation, centrifugation
Revolving speed is 4000r/min, and centrifugation time is 18 min, and the temperature of alcohol precipitation is 4 DEG C, and the time of alcohol precipitation is 12h, obtains three kinds of differences
Fraction polysaccharide precipitating；
(5) polysaccharide is dry, will obtain three kinds of lentinans after the polysaccharide precipitation drying of three kinds of different components respectively, A polysaccharide, B are more
Sugar, C polysaccharide, the temperature of drying are 50 DEG C.
After tested, the present embodiment is using 30%, 55%, 70% concentration of alcohol classification alcohol precipitation three kinds of lentinan components of gained
Yield difference, 9.71%, 3.58%, 1.05%, total polysaccharide yield be 14.34%；A polysaccharide, B polysaccharide, polyoses content point in C polysaccharide
It Wei 28.39%, 64.38%, 52.77%.
Wherein the beta glucan content in 70% concentration of alcohol alcohol precipitation polysaccharide component is 25.84%, protein content is
7.38%, viscosity average molecular weigh 93.32KDa, the yield that stimulated in vitro macrophage discharges NO is 47.00 μm of ol/mL, external right
The inhibiting rate of liver cancer cells is that 19.39%, DPPH Scavenging activity is 28.34%, Hydroxyl radical-scavenging ability be 11.80%, super oxygen from
Sub- Scavenging activity is 7.08%.
Have in embodiment 1 to embodiment 4 using the extraction that the mushroom fruiting body of four-stage carries out lentinan as raw material
Very high yield.
Each polysaccharide component of mushroom nutritional ingredient rich in embodiment 1 to embodiment 4, wherein each polysaccharide component
Rate range is 1.05%-14.60%, 20% ethyl alcohol alcohol precipitation polysaccharide yield highest；The polyoses content range of each polysaccharide component is
32.77%-70.29%, 50% ethyl alcohol alcohol precipitation polyoses content highest；The protein content range of each polysaccharide component is 7.38%-
12.83%, protein content highest in 20% ethyl alcohol alcohol precipitation polysaccharide；70% ethyl alcohol alcohol precipitation polysaccharide yield highest of beta glucan；It is viscous to divide equally
Son 20% ethyl alcohol alcohol precipitation polysaccharide highest of amount, the molecular weight of all polysaccharide components is within the components range of effective active；20% second
The yield highest of antioxidant activity and stimulated in vitro macrophage the release NO of alcohol alcohol precipitation polysaccharide；The body of 70% ethyl alcohol alcohol precipitation polysaccharide
Outside to the inhibiting rate highest of liver cancer cells.
Using different ethanol concentration, using the mushroom fruiting body of different growing stages as raw material, can obtain different bioactivity,
The yield of the lentinan that polyoses content is different, molecular weight is different, polysaccharide is high, sufficiently promotes the application value of lentinan, has
Conducive to the classification application of lentinan.Identical mushroom leaching liquor can propose a variety of polysaccharide precipitations, the second of various concentration simultaneously
The activity and component for the lentinan that alcohol extracting is taken out are different, can be improved with the simplification production technology of high degree to raw materials of letinous edodes
The recovery rate of middle polysaccharide saves the usage amount of raw materials of letinous edodes.Meet the exploitation demand of different food, health care product, is sufficiently promoted
The application value of lentinan, while effectively reducing the preparation cost of lentinan.
The present invention is not limited to above-mentioned specific embodiment, and the invention may be variously modified and varied.All foundations
Technical spirit of the invention should be included in the present invention to embodiment of above any modification, equivalent replacement, improvement and so on
Protection scope.
Claims (9)
1. a kind of preparation method of difference bioactivity lentinan, which comprises the following steps:
(1) it crushes, 60-80 mesh screen is crossed after mushroom fruiting body dry, pulverize, obtains mushroom powder；
(2) extract, take mushroom powder that pure water is added and obtain mixed liquor, the mushroom powder, pure water ratio be 1:20-40 m/v,
It heats mixed liquor and is extracted for the first time at 90-100 DEG C；
(3) it filters, the first leaching liquor is obtained after filtering, filter residue carries out secondary extraction, obtains the second leaching liquor, merges the first extraction
Liquid, the second leaching liquor obtain extraction filtrate；
(4) it is classified alcohol precipitation, extraction filtrate obtains concentrate after being concentrated under reduced pressure, ethyl alcohol is added several times, successively makes final second
Determining alcohol is 20-70% mL/mL, carries out classification alcohol precipitation, obtains the polysaccharide precipitation of a variety of different component contents；
(5) polysaccharide is dry, respectively dries a variety of polysaccharide precipitations, obtains a variety of lentinans.
2. a kind of preparation method of different bioactivity lentinans according to claim 1, which is characterized in that
The temperature of step (1) described drying is 40-60 DEG C, and the dry time is 6-12h.
3. a kind of preparation method of different bioactivity lentinans according to claim 1 or 2, which is characterized in that step
Suddenly the time of (2) described extraction is 2-3h.
4. a kind of preparation method of different bioactivity lentinans according to claim 1, which is characterized in that
The cocnentration factor of step (4) described reduced pressure is 3-5:1 v/m.
5. a kind of preparation method of different bioactivity lentinans according to claim 1, which is characterized in that step
(4) temperature of the alcohol precipitation is 4-5 DEG C, and the time of alcohol precipitation is 6-12h.
6. a kind of preparation method of different bioactivity lentinans according to claim 1, which is characterized in that step
(5) further include centrifugation before the drying, collect precipitating.
7. a kind of preparation method of different bioactivity lentinans according to claim 6, which is characterized in that it is described from
The revolving speed of the heart is 4000-5000 r/min, and centrifugation time is 15-20 min.
8. a kind of preparation method of different bioactivity lentinans according to claim 1, which is characterized in that
The temperature of the drying is 45-58 DEG C.
9. a kind of preparation method of different bioactivity lentinans according to claim 1, which is characterized in that the perfume (or spice)
Massee fruiting bodies are one in the fructification of mushroom flower bud phase, the fructification of immature phase, the fructification in maturity period, the fructification of parachute-opening phase
Kind is a variety of.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607012A (en) * | 2020-05-29 | 2020-09-01 | 西南林业大学 | Paeonia suffruticosa polysaccharide extract and preparation method and application thereof |
| CN113024677A (en) * | 2019-12-25 | 2021-06-25 | 金陵药业股份有限公司 | Preparation method of low-molecular-weight lentinan |
| CN113968919A (en) * | 2021-11-25 | 2022-01-25 | 浙江百山祖生物科技有限公司 | Edible fungus extract without auxiliary materials and preparation method thereof |
| CN117487036A (en) * | 2023-10-12 | 2024-02-02 | 上海市农业科学院 | Lentinan rich in beta-glucan and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004033502A1 (en) * | 2002-10-08 | 2004-04-22 | Ricom Corporation | Chitosan-containing polysaccharide, process for producing the same and use thereof |
| CN103724447A (en) * | 2013-12-31 | 2014-04-16 | 中国科学院长春应用化学研究所 | Extraction and classification method of lentinan |
| CN104892791A (en) * | 2015-06-29 | 2015-09-09 | 成都大学 | Lentinan and extraction and purification method thereof |
-
2019
- 2019-07-17 CN CN201910646558.XA patent/CN110256598A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004033502A1 (en) * | 2002-10-08 | 2004-04-22 | Ricom Corporation | Chitosan-containing polysaccharide, process for producing the same and use thereof |
| CN103724447A (en) * | 2013-12-31 | 2014-04-16 | 中国科学院长春应用化学研究所 | Extraction and classification method of lentinan |
| CN104892791A (en) * | 2015-06-29 | 2015-09-09 | 成都大学 | Lentinan and extraction and purification method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113024677A (en) * | 2019-12-25 | 2021-06-25 | 金陵药业股份有限公司 | Preparation method of low-molecular-weight lentinan |
| CN111607012A (en) * | 2020-05-29 | 2020-09-01 | 西南林业大学 | Paeonia suffruticosa polysaccharide extract and preparation method and application thereof |
| CN113968919A (en) * | 2021-11-25 | 2022-01-25 | 浙江百山祖生物科技有限公司 | Edible fungus extract without auxiliary materials and preparation method thereof |
| CN117487036A (en) * | 2023-10-12 | 2024-02-02 | 上海市农业科学院 | Lentinan rich in beta-glucan and preparation method thereof |
| CN117487036B (en) * | 2023-10-12 | 2024-05-31 | 上海市农业科学院 | Lentinan rich in beta-glucan and preparation method thereof |
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