CN106539739B - Nostoc sphaeroids kutz cotton mask and preparation method and application thereof - Google Patents

Abstract

The invention relates to a nostoc sphaeroides cotton facial mask and a preparation method and application thereof; the preparation method comprises the steps of tissue mashing, nutrient component adding, colloid mill homogenizing, ultrasonic crushing, mold pouring, freezing, drying and the like. The invention finds a convenient and rapid method for making the nostoc sphaeroides cotton mask, which is simple in process and environment-friendly.

Description

Nostoc sphaeroids kutz cotton mask and preparation method and application thereof

Technical Field

The invention relates to a nostoc sphaeroides cotton facial mask and a preparation method and application thereof.

Background

Nostoc sphaeroides K ü tzing, commonly known as Nostoc sphaeroides, loosestrife, Auricularia, Tremella, belonging to the class of cyanobacteria, Nostoc sphaeroides K ü tzing, is a precious freshwater nitrogen-fixing cyanobacteria in the world, has the highest yield in Hubei, has been reported to be distributed in Africa and the like in a small amount in paddy fields mainly distributed in mountain areas in China.

Phycobiliprotein as a light-harvesting pigment protein is widely distributed in blue algae. Phycobiliprotein has the obvious function of eliminating free radicals and active oxygen, and has also been reported to have the obvious anti-inflammatory activity. Therefore, the application range of the phycobiliprotein is wide, and the development and utilization values are high. Nostoc sphaeroides contains abundant phycobiliprotein, the content of the Nostoc sphaeroides is equivalent to that of spirulina, the potential commercial value of Nostoc sphaeroides per se is increasingly shown, and therefore the development and the utilization of Nostoc sphaeroides have attracted the attention of governments and people.

Nostoc sphaeroids kutz is applied to the fields of washing products and skin care products. The developed products comprise nostoc moisturizing skin care products, nostoc washing products and the like. The nostoc sphaeroides cotton material prepared by the invention does not have a colloid sheath structure, and a porous sponge material of the nostoc sphaeroides cotton material can be developed and applied in the field of masks.

The facial mask mainly has four types, namely a paste type, a tearing type, a jelly type and a wet tissue type. The mud paste type facial mask is commonly an alga facial mask, a mud facial mask and the like, the tear type facial mask is the nasal paste special for blackheads, the jelly type is the best known as a sleep facial mask, and the wet tissue type is generally a facial mask paper which is packaged by a single sheet and is soaked with beauty treatment liquid. With the development of beauty science and technology, a wet tissue type facial mask made of silk appears. The wet tissue type facial mask soaked with the skin care lotion accounts for a large share in the market, the facial mask paper can be divided into silk, all cotton, terylene, blended fabric, biological fiber and the like according to the material, the biological fiber facial mask paper has the best effect, and the blended facial mask paper is the first time. The material of the facial mask paper can influence the comfort and the effect of the facial mask. Natural pure cotton-based facial mask paper is the most popular material in recent years, and synthetic fiber materials are inferior. The facial mask paper with a certain thickness and weight can absorb sufficient moisturizing essence, and the wrapping effect of the facial mask paper on skin in a pressurized sealing mode is better. The biological fiber facial mask paper has the best effect and is generally used in professional beauty salons, and because the biological fiber facial mask paper is expensive, the products at the price are difficult to be burdened by general people.

The nostoc sphaeroides can be processed into a pure natural cotton biological mask to be developed and applied. The material can realize natural combination of functional nutritional skin care components (mainly comprising nostoc sphaeroides biliprotein) and biological mask carrier, and has long shelf life in dry state, convenient carrying, and no preservative.

At present, no dry pure natural nostoc sphaeroides biological material facial mask combining skin care components and a facial mask carrier is available, the facial mask is completely a pure natural biological material and is rich in biological active components with the effects of diminishing inflammation and nourishing and protecting skin.

Disclosure of Invention

The invention aims to provide a nostoc sphaeroides cotton facial mask and a preparation method and application thereof. The invention finds a convenient and rapid method for making the nostoc sphaeroides cotton mask, which is simple in process and environment-friendly.

The technical scheme of the invention is as follows:

a method for preparing nostoc sphaeroides cotton facial mask comprises mashing nostoc sphaeroides, adding nutritional ingredients, homogenizing with colloid mill, ultrasonic crushing to obtain algae slurry mixture, pouring the algae slurry into a mold, freezing at-10 deg.C in dark place, and drying.

Preferably, the nutrient components comprise one or more of liquid milk, honey, egg white and other mask nutrient components; further preferably, the nutrient content is 5-10ml per 100g algae slurry mixture.

Preferably, the nostoc sphaeroides is fresh nostoc sphaeroides or dried nostoc sphaeroides rehydrated or dried nostoc sphaeroides, and the dried nostoc sphaeroides is crushed and sieved by a sieve with the mesh size being more than or equal to 80 meshes.

Preferably, the nostoc sphaeroides dried product is rehydrated, the nostoc sphaeroides is soaked in purified water or distilled water until the weight of the nostoc sphaeroides is increased by 16-50 times, more preferably 30-40 times after the nostoc sphaeroides absorbs water, and then the recovered nostoc sphaeroides is mashed into tissues.

Pulverizing dried Nostoc sphaeroids Kutz for 3-8 min, sieving with 80 mesh sieve, adding purified water or distilled water into the sieved fine powder, and rehydrating with water at a ratio of 1Kg to 16L-1Kg to 50L.

Generally, the nostoc sphaeroides can be mashed into algae slurry by a tissue crusher, and the treatment time depends on the performance of the machine.

Preferably, the tissue is mashed by a tissue masher at a rotation speed of 10-120r/min, and the algal body tissue is mashed to prepare algal slurry; then adding nutrient components into the algae slurry.

Homogenizing the tissue-mashed algae slurry by using a colloid mill, preferably at 1450-. Preferably, the ultrasonic crushing treatment condition is that the frequency is 20-25KHz, ultrasonic treatment is carried out for 5s every 10s, and ultrasonic treatment is carried out for 5-10 min. Experiments show that the ultrasonic treatment is more favorable for breaking cell walls and enhancing the homogenization effect.

Preferably, a filtration step is also included after ultrasonication. According to the requirement, the mashed algae can be filtered by a sieve with 80 meshes or more. Experiments show that the multifunctional cotton with small, uniform and fine apertures can be obtained by selecting a screen with high mesh number (preferably 80-150 meshes); the coarse multifunctional cotton with larger aperture can be obtained by selecting a screen with low mesh number (preferably 20-80 meshes). If the filtration is not carried out, the obtained multifunctional cotton has larger aperture and is rougher.

And (3) carrying out ultrasonic crushing or further filtering to prepare an algae slurry mixture.

Preparing nostoc sphaeroides cotton facial mask, pouring the algae slurry mixture into a facial mould, wherein the pouring thickness can be less than or equal to 1cm, preferably about 0.3-0.5 cm; different surface molds can be selected for pouring according to the requirement.

Preferably, the freezing is performed under dark or light-shielding conditions.

The phycobiliprotein can be effectively prevented from fading and decomposing when being frozen in dark or dark.

Preferably, the freezing temperature is-10 ℃ or lower.

Generally, the low temperature environment can be realized by adjusting the temperature of a common refrigerator, freezer or cold storage to a freezing gear; if the requirement on the freezing speed is higher, the liquid nitrogen freezing method can be adopted to realize quick freezing.

Preferably, the nostoc sphaeroides cotton facial mask is collected and stored in a dark or dark dry environment.

Specifically, the preparation method of the nostoc sphaeroides cotton facial mask comprises the following steps:

mashing tissue of Nostoc sphaeroids Kutz; adding nutrient components into the mashed algae slurry, homogenizing with colloid mill, ultrasonic crushing, and filtering with 80-150 mesh sieve; preparing an algae slurry mixture; pouring the obtained algae slurry mixture into a surface mold, wherein the pouring thickness is 0.3-0.5 cm; placing the surface mold with the poured algae slurry at-10 deg.C in dark place, freezing, and drying;

wherein the tissue mashing rotating speed is 10-120 r/min; the homogenizing rotation speed of the colloid mill is 1450 and 3200r/min, and the processing fineness is 2-50 mu m; the ultrasonic crushing condition is that the frequency is 20-25KHz, and the ultrasonic treatment is carried out for 5s at intervals of 10s for 5-10 min;

the nutrient components comprise one or more of liquid milk, honey and egg white; the nutrient content is 5-10ml per 100g algae slurry mixture.

The invention also comprises the nostoc sphaeroides cotton facial mask prepared by the method; its main nutrient components, phycobiliprotein, etc. are stored in its own pores.

The invention also discloses the application of the nostoc sphaeroides mask prepared by the method in preparing the mask. The application is, for example, preparing a dry pure natural biological material facial mask.

When in use, the facial mask can also be soaked in clear water, or further soaked in materials such as milk, honey, cucumber juice, egg white, red wine, yoghourt, banana puree and the like, or soaked in a mixture of the materials and then applied to the face.

The nostoc sphaeroides cotton mask is a porous spongy pure natural biological material. After the material is soaked in water, the porous structure is filled with nutrient components such as phycobiliprotein. The crude protein extract rich in phycobiliprotein has obvious antioxidant and antiphlogistic effects, and is one kind of natural nutritious skin care liquid. The porous sponge structure is a pure natural skin care carrier material.

The nostoc sphaeroides cotton mask is a biological material with multiple functions, and is a porous spongy structure formed by processing nostoc sphaeroides through mashing firstly and then freezing, so that the molecular composition pattern of viscous and uniform algae slurry after mashing is changed, and no chemical reagent is added in the preparation process of the material, so that the nostoc sphaeroides cotton mask belongs to a pure natural biological material.

The invention has the advantages that:

(1) novel product processing thought

Freezing after cell wall breaking, the colloid component (mainly macromolecular protein and macromolecular polysaccharide) of nostoc sphaeroides is insoluble in water, the main functional nutrient component phycobiliprotein is soluble in water, after the whole biological material is frozen, the phycobiliprotein and water are frozen together by utilizing the water solubility of the phycobiliprotein, meanwhile, the water-insoluble biological macromolecules approach to each other, so that the chemical bonds of the adjacent macromolecules are contacted with each other to form new and more stable combination, namely, the polymerization reaction between the biological macromolecules is generated, the separation of the phycobiliprotein and other water-insoluble biological macromolecules is realized, then the water is removed by utilizing a drying method, the phycobiliprotein is left in the pores where the ice crystals formed by the original water are located, namely, the phycobiliprotein is stored in the pores of the dried cotton structure body, so that the cotton material formed by the nostoc sphaeroides itself becomes the storage carrier of the phycobiliprotein released by the nostoc sphaeroides itself, the phycobiliprotein of the cotton material can be released continuously after the cotton material is soaked by water.

The method has unique process, does not need pressing, and can realize the purpose of adhering the algae pulp into the cotton material by a freezing method.

(2) The product safety is outstanding

There is no pure natural plant facial mask on the market. The mask prepared by the invention is a pure natural biological material: the cotton material is self organism of mashed nostoc; the phycobiliprotein is natural pigment protein synthesized by nostoc sphaeroides per se; during the manufacturing process, no chemical agent is added. Because the cotton material belongs to the dry state, the phycobiliprotein is also in the dry state, and the phycobiliprotein is stored in the pores of the dry cotton, no adhesive or preservative is added, only clean water is needed to soak the cotton material when the cotton material is used, and the safety performance is extremely high.

(3) The product has outstanding effect

Fully retains the biological activity of the nostoc sphaeroides gallbladder protein. The mask contains pure natural nutritional ingredients in algae, most particularly high-content water-soluble phycobiliprotein, and the phycobiliprotein can be released only by immersing the mask sheet in clean clear water, has the effects of resisting oxidation, resisting radiation, resisting aging, resisting bacteria, diminishing inflammation, resisting allergy and the like, and can effectively nourish and clean the skin.

(4) The product has novel style

The phycobiliprotein with the biological active component of the phycobiliprotein is stored in pores of a cotton structure formed by the phycobiliprotein, so that the phycobiliprotein has stable biological activity, outstanding effect and high use safety. The nostoc sphaeroides cotton mask porous spongy biomaterial is rich in phycobiliprotein nutritional health-care ingredients, is soaked by clean water and then is released to form pure natural skin lotion beneficial to skin health, and the spongy structure of the nostoc sphaeroides cotton mask porous spongy biomaterial plays a role of a carrier. Therefore, the nostoc sphaeroides cotton facial mask realizes the perfect combination of skin care nutrient components and facial mask carriers, and is a dry-state pure natural biological material functional facial mask.

The invention has been verified by a plurality of tests, and the result shows that after the nostoc sphaeroides is firstly smashed into algae slurry and then is frozen, the nostoc sphaeroides can really and stably generate a porous spongy structure, and the porous structure is rich in functional nutrient components mainly comprising phycobiliprotein, and the crude protein extract can be released after being soaked in water.

Drawings

FIG. 1 is a graph showing the microscopic morphology of Nostoc sphaeroids Kutz, Nostoc commune and Nostoc flagelliforme in Experimental example 1.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.

Example 1

A preparation method of nostoc sphaeroides cotton facial mask comprises the following steps:

adopting a fresh nostoc sphaeroides product as an initial raw material, mashing tissues of spherical algae in the fresh nostoc sphaeroides product, and mashing the tissues by adopting a tissue mashing machine (the rotating speed is 80 r/min); performing wet ultrafine grinding on the mashed tissue by using a colloid mill (rotating speed of 2800r/min, processing fineness of 50 μm), and performing shearing/grinding/high-speed stirring acting force of the colloid mill to realize cell wall breaking and strong dispersion and mixing of algae slurry; filtering with 100 mesh screen; carrying out ultrasonic crushing treatment for 5min under the condition of 25KHz (5 s of ultrasonic treatment every 10 s); preparing algae slurry; the obtained algae slurry is poured into a surface mold, and the pouring thickness is 0.4 cm. Placing the mold with the poured algae slurry in a dark place at-10 deg.C, freezing, thawing at normal temperature, and drying at 35 deg.C under hot air circulation condition to obtain nostoc sphaeroides cotton facial mask. The cotton facial mask can be soaked in clear water, or soaked in materials such as milk, Mel, succus Cucumidis Sativi, ovum gallus Domesticus album, red wine, yogurt, and fructus Musae paste, or soaked in mixture of the above materials and applied to face.

Example 2

A preparation method of nostoc sphaeroides cotton facial mask comprises the following steps:

adopting a fresh nostoc sphaeroides product as an initial raw material, mashing tissues of spherical algae in the fresh nostoc sphaeroides product, and mashing the tissues by adopting a tissue mashing machine (the rotating speed is 80 r/min); adding liquid milk, honey, egg white or other nutrient components of the facial mask into the tissue-mashed algae slurry, wherein 1ml of the nutrient components is added into each 100g of the algae slurry; performing wet ultrafine grinding by using a colloid mill (rotating speed of 2800r/min, processing fineness of 50 μm), and performing strong dispersion and mixing of cell wall breaking and algae slurry by using shearing/grinding/high-speed stirring action of the colloid mill; carrying out ultrasonic crushing treatment for 5min under the condition of 25KHz (5 s of ultrasonic treatment every 10 s); filtering with 100 mesh screen; preparing an algae slurry mixture; pouring the obtained algae slurry mixture into a surface mold, wherein the pouring thickness is 0.4 cm. Placing the mold with the poured algae slurry in a dark place at-10 deg.C, freezing, thawing at normal temperature, and drying at 35 deg.C under hot air circulation condition to obtain nostoc sphaeroides cotton facial mask. The cotton facial mask can be soaked in clear water, or further soaked in materials such as milk, Mel, succus Cucumidis Sativi, ovum gallus Domesticus album, red wine, yogurt, and fructus Musae paste, or soaked in mixture of the above materials and applied to face.

Example 3

A preparation method of nostoc sphaeroides cotton facial mask comprises the following steps:

adopting a fresh nostoc sphaeroides product as an initial raw material, mashing tissues of spherical algae in the fresh nostoc sphaeroides product, and mashing the tissues by adopting a tissue mashing machine (the rotating speed is 80 r/min); adding liquid milk, honey, egg white or other nutrient components of the facial mask into the tissue-mashed algae slurry, wherein 1ml of the nutrient components is added into each 100g of the algae slurry; performing wet ultrafine grinding by using a colloid mill (rotating speed of 2800r/min, processing fineness of 50 μm), and performing strong dispersion and mixing of cell wall breaking and algae slurry by using shearing/grinding/high-speed stirring action of the colloid mill; carrying out ultrasonic crushing treatment for 5min under the condition of 25KHz (5 s of ultrasonic treatment every 10 s); filtering with 100 mesh screen; preparing an algae slurry mixture; pouring the obtained algae slurry mixture into a surface mold, wherein the pouring thickness is 0.4cm, and placing the surface mold with the algae slurry in a vacuum freeze dryer for vacuum freeze drying to obtain the nostoc sphaeroides cotton facial mask. The cotton facial mask can be soaked in clear water, or further soaked in materials such as milk, Mel, succus Cucumidis Sativi, ovum gallus Domesticus album, red wine, yogurt, and fructus Musae paste, or soaked in mixture of the above materials and applied to face.

Example 4

A preparation method of nostoc sphaeroides cotton facial mask comprises the following steps:

adopting a dry nostoc sphaeroides product as an initial raw material, rehydrating the dry nostoc sphaeroides product, soaking nostoc sphaeroides in purified water or distilled water until the weight of the nostoc sphaeroides product is increased by 40 times after the nostoc sphaeroides product absorbs water, and mashing the tissue of the algae by using a tissue mashing machine (the rotating speed is 80 r/min); adding liquid milk, honey, egg white or other nutrient components of the facial mask into the tissue-mashed algae slurry, and adding 5ml of the nutrient components into every 100g of algae slurry; performing wet ultrafine grinding by using a colloid mill (rotating speed of 2800r/min, processing fineness of 50 μm), and performing strong dispersion and mixing of cell wall breaking and algae slurry by using shearing/grinding/high-speed stirring action of the colloid mill; carrying out ultrasonic crushing treatment for 5min under the condition of 25KHz (5 s of ultrasonic treatment every 10 s); filtering with 100 mesh screen; preparing an algae slurry mixture; pouring the obtained algae slurry mixture into a face mold, pouring the thickness of 0.4cm, freezing the face mold with the algae filaments in the dark at-10 deg.C, thawing at normal temperature, and drying at 35 deg.C under hot air circulation condition to obtain the final product. The cotton facial mask can be soaked in clear water, or further soaked in materials such as milk, Mel, succus Cucumidis Sativi, ovum gallus Domesticus album, red wine, yogurt, and fructus Musae paste, or soaked in mixture of the above materials and applied to face.

Example 5

A preparation method of nostoc sphaeroides cotton facial mask comprises the following steps:

the nostoc sphaeroides dry powder is adopted as an initial raw material form, and the preparation process of the nostoc sphaeroides dry powder is as follows: pulverizing dried Nostoc sphaeroids Kutz for 5min, and sieving with 80 mesh sieve. Adding purified water or distilled water into the sieved fine powder for rehydration, wherein the rehydration ratio of the nostoc sphaeroids kutz dry powder to the water is 1Kg to 32L. Adding liquid milk, honey, egg white or other nutrient components of the facial mask into the obtained algae slurry, and adding 5ml of the nutrient components into each 100g of algae slurry; then carrying out wet ultrafine grinding on the algae slurry by using a colloid mill (the rotating speed is 2800r/min, the processing fineness is 50 mu m), and realizing cell wall breaking and strong dispersive mixing of the algae slurry by using the shearing/grinding/high-speed stirring acting force of the colloid mill; carrying out ultrasonic crushing treatment for 5min under the condition of 25KHz (5 s of ultrasonic treatment every 10 s); filtering with 100 mesh screen; obtaining an algae slurry mixture; pouring the obtained algae slurry mixture into a face mold, pouring the thickness of the algae slurry mixture to be 0.4cm, placing the face mold with the algae slurry in the place with the temperature of minus 10 ℃ and being away from the sun for freezing, melting at normal temperature, placing the whole body in the hot air circulation condition of 35 ℃ for drying, and obtaining the nostoc sphaeroides cotton facial mask. The cotton facial mask can be soaked in clear water, or further soaked in materials such as milk, Mel, succus Cucumidis Sativi, ovum gallus Domesticus album, red wine, yogurt, and fructus Musae paste, or soaked in mixture of the above materials and applied to face.

Comparative example 1

Adopting a fresh nostoc sphaeroides product as an initial raw material, mashing tissues of spherical algae in the fresh nostoc sphaeroides product, and mashing the tissues by adopting a tissue mashing machine (the rotating speed is 80 r/min); adding 2% agar powder or 10% cassava modified starch or 5% guar gum or 2.5% carrageenan or 3% sodium alginate or 20% gellan gum, adding liquid milk, honey or egg white and other facial mask nutrients, and adding 5ml of the nutrients to 100g of algae slurry; then the mixture is subjected to wet ultrafine grinding by using a colloid mill (the rotating speed is 2800r/min, the processing fineness is 50 mu m), and the shearing/grinding/high-speed stirring acting force of the colloid mill is utilized to realize the strong dispersion and mixing of the cell wall breaking and algae pulp mixture; filtering with 100 mesh screen; carrying out ultrasonic crushing treatment for 5min under the condition of 25KHz (5 s of ultrasonic treatment every 10 s); the prepared algae slurry mixture does not have the cotton forming characteristic after being poured into a surface type mould.

Comparative example 2

The nostoc sphaeroides dry powder is adopted as an initial raw material form, and the preparation process of the nostoc sphaeroides dry powder is as follows: pulverizing for 3 min, adding purified water or distilled water, and rehydrating with 1Kg of dried powder of nostoc sphaeroids kutz and 32L of water. Adding liquid milk, honey, egg white or other nutrient components of the facial mask into the obtained algae slurry, and adding 5ml of the nutrient components into each 100g of algae slurry; carrying out ultrasonic crushing treatment for 5min under the condition of 25KHz (5 s of ultrasonic treatment every 10 s); the prepared algae slurry mixture does not have the cotton forming characteristic after being poured into a surface type mould.

Comparative example 3

The procedure of example 1 was repeated except that Nostoc commune, a. kohlrabi, Nostoc commune, lichen, Nostoc commune, Geotrichum japonicum, Ralstonia rex, Geotrichum japonicum, etc., which are combinations of fungi and algae and belong to the family Candida mycoceae, was used as a starting material. As a result, the cotton characteristics were poor and the stability was poor.

Comparative example 4

Nostoc commune var. flagelliforme, a synonym Nostocflagelliforme, called Nostocflagelliforme, an alga of Nostocfagelliforme, is an objective Nostocflamda, was used as a raw material, and the operation was carried out in the same manner as in example 1. As a result, cotton formation was not possible.

Experimental example 1

The experimental study on the cotton forming characteristics of nostoc sphaeroides of example 1 and nostoc commune of comparative examples 3-4 was carried out, and the results are shown in fig. 1(a. nostoc sphaeroides-example 1; b. nostoc commune-comparative example 3; c. nostoc commune-comparative example 4).

1) Comparison of microscopic appearance of Nostoc sphaeroids Kutz and other two Nostoc commune (Nostoc sphaeroids Kutz and Nostoc flagelliforme)

FIG. 1-a is a structural view of the nostoc sphaeroides cotton structure obtained by the method of example 1, wherein the cotton body is rich in cavities formed by a large number of bubbles, the cavities have uniform sizes and are uniformly distributed, and the overall structure of the nostoc sphaeroides cotton material also shows uniform characteristics; FIG. 1-b shows the Auricularia auricula preparation obtained in comparative example 3, which can form cotton with very poor structural toughness in a short time, wherein the cotton also contains voids formed by bubbles, but the voids have significant size difference, very small number and very uneven distribution; the hair weeds were treated by the method of comparative example 4, and a cotton body mask was not formed, and fig. 1-c are the microscopic structures thereof. Fig. 1 shows a microscopic structure diagram of three nostoc, which fully shows the difference of texture of three nostoc treated by the method of the invention, the nostoc pulp has unique colloid performance before being formed into cotton, and only nostoc can form a stable high-quality cotton-shaped facial mask.

2) Comparison of Nostoc sphaeroids Kutz with population-state algae

Laver, kelp and undaria pinnatifida were treated according to the method of example 1, respectively, and none of the three seaweeds could form a cotton mask.

3) Comparison of Nostoc sphaeroides with unicellular algae other than Nostoc sphaeroides

Since the tackiness of the unicellular algae plants was significantly lower than nostoc sphaeroides, no cotton mask structure could be formed after the cell population of the unicellular algae plants was treated according to the method of example 5.

4) Comparison result of Nostoc sphaeroids Kutz with fungus fruiting body Tremella and Auricularia

After the fungus fruiting bodies of tremella and agaric are treated by the method of example 1, no cotton mask structure is formed.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (9)

1. A preparation method of nostoc sphaeroides cotton facial mask is characterized by comprising the steps of mashing nostoc sphaeroides tissue, adding nutrient components, homogenizing by a colloid mill, carrying out ultrasonic crushing to obtain an algae slurry mixture, pouring the algae slurry mixture into a mold, freezing in the dark at the temperature of below-10 ℃, and drying to obtain the nostoc sphaeroides cotton facial mask; the nutrient components comprise one or more of liquid milk, honey and egg white;

wherein the tissue mashing rotating speed is 10-120 r/min; the homogenizing rotation speed of the colloid mill is 1450 and 3200r/min, and the processing fineness is 2-50 mu m; the ultrasonic crushing condition is that the frequency is 20-25KHz, and the ultrasonic treatment is carried out for 5s at intervals of 10s for 5-10 min.

2. The method according to claim 1, wherein the nutrient is contained in an amount of 5-10ml per 100g of the algal slurry mixture.

3. The method according to claim 1 or 2, wherein the nostoc sphaeroides is a fresh nostoc sphaeroides or a dried nostoc sphaeroides rehydrated.

4. The method of claim 1 or 2, further comprising a step of filtration after the ultrasonication; the mesh number of the filter screen is 80-150 meshes.

5. The preparation method according to claim 1 or 2, comprising pouring the obtained algae slurry into a surface mold, wherein the pouring thickness is less than or equal to 1 cm.

6. The method of claim 5, wherein the cast thickness is 0.3-0.5 cm.

7. The method of manufacturing according to claim 1 or 2, comprising the steps of: mashing tissue of Nostoc sphaeroids Kutz; adding nutrient components into the mashed algae slurry, homogenizing with colloid mill, ultrasonic crushing, and filtering with 80-150 mesh sieve; preparing an algae slurry mixture; pouring the obtained algae slurry mixture into a surface mold, wherein the pouring thickness is 0.3-0.5 cm; placing the surface mold with the poured algae slurry at-10 deg.C in dark place, freezing, and drying;

the nutrient components comprise one or more of liquid milk, honey and egg white; the nutrient content is 5-10ml per 100g algae slurry mixture.

8. A nostoc sphaeroides cotton facial mask prepared by the method of any one of claims 1 to 7.

9. The use of the nostoc sphaeroides cotton mask as claimed in claim 8 for preparing a mask.

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