CN106344420B - A kind of nostoc Multifunctional cotton and the preparation method and application thereof - Google Patents

A kind of nostoc Multifunctional cotton and the preparation method and application thereof Download PDF

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CN106344420B
CN106344420B CN201610868282.6A CN201610868282A CN106344420B CN 106344420 B CN106344420 B CN 106344420B CN 201610868282 A CN201610868282 A CN 201610868282A CN 106344420 B CN106344420 B CN 106344420B
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nostoc
preparation
cotton
algae slurry
poured
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CN106344420A (en
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安彦君
钟责平
董美丽
刘红英
周丰
王卫
熊斌
王远忠
陈牧
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HUNAN YANDI BIOLOGICAL ENGINEERING Co Ltd
CHANGDE YANDI BIOLOGICAL TECHNOLOGY Co Ltd
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HUNAN YANDI BIOLOGICAL ENGINEERING Co Ltd
CHANGDE YANDI BIOLOGICAL TECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0212Face masks
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/168Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645Proteins of vegetable origin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/70Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A61K9/7023Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/10Washing or bathing preparations

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Dermatology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to a kind of nostoc Multifunctional cottons and the preparation method and application thereof;The preparation method includes tissue mashing, algae slurry filtering, ultrasonication, casting mold, freezing, drying and other steps.The present invention has found a kind of method that can conveniently make nostoc Multifunctional cotton, simple process, and environmentally protective.

Description

A kind of nostoc Multifunctional cotton and the preparation method and application thereof
Technical field
The present invention relates to a kind of nostoc Multifunctional cottons and the preparation method and application thereof.
Background technique
Nostoc (latin name: Pogostemon auricularius (L.) Kassk;Scientific name: intend spherical nostoc Nostoc sphaeroides K ü tzing), it is commonly called as beauty's rice, day rosetangle, loosestrife, water agaric, field agaric belong to Cyanophyceae, Nostocaceae.Nostoc is a kind of fresh water Azotica of preciousness.The distribution of nostoc in the world is extremely rare, in China master It is distributed in the paddy field in mountain area, the yield highest of Hefeng of Hubei, it was reported that also there is a small amount of distribution on the ground such as Africa.Nostoc exists China is with a long history, is not only one of traditional delicacies full of nutrition, and have considerable medical value and health-care efficacy.Pueraria lobota Celestial meter Fu Han amino acid, protein, vitamin C, vitamin B1 and B2, several mineral materials, the height of calcium content pole in vegetables To be rare, polysaccharide has good beauty and skin care effect.It is recorded according to Compendium of Material Medica and supplementary Amplifications of the Compendium of Materia Medica etc., nostoc Yctalopia, rectal prolapse, burn and scald etc. can be treated.In addition, introducing according to " national Chinese herbal medicine compilation ", nostoc is cold in nature, lightly seasoned, has clear The medicinal healths effects such as heat, convergence, QI invigorating, improving eyesight.Therefore, nostoc is a kind of natural algae resource that economic value is high.
It is widely distributed in cyanobacteria that phycobniliprotein as one kind catches photochromic fibroin.Phycobniliprotein has significant eliminate certainly By the effect of base and active state oxygen, also it has been reported that phycobniliprotein has significant antiphlogistic activity.Therefore the application of phycobniliprotein Wide scope has very high exploitation, utility value.Nostoc phycobniliprotein rich in, the phase of content and spirulina When its own potential commercial value also increasingly shows, therefore its development and utilization has caused government and people widely to weigh Depending on.
It is at present to the application and development of nostoc to be confined to catering trade, nutrient and healthcare products, washing product and skin care field more. The product having been developed that includes nostoc nutritious food, nostoc Moisturizer, nostoc washing product etc..In catering trade, make It is difficult tasty for a kind of rare food materials since its spherome surface is the stronger colloid epitheca of one layer of toughness.Present invention production Nostoc Multifunctional cotton be not present colloid epitheca structure, porous spongy material is also a kind of natural green food, because It is easily tasty when this is as food materials.
The form of facial mask mainly has mud paste type, tear-pull, gel-type, four kinds of hygenic towelette type.Mud paste type facial mask common are Seaweed mask, sludge facial mask etc., it is exactly the dedicated nose patch of blackhead that tear-pull facial mask is most common, gel-type with sleep mask most To be famous, hygenic towelette formula is generally exactly the face paper for infiltrating beautifying liquid of mmic package.Along with the development of beauty science and technology, out Show a kind of using silk as the hygenic towelette type facial mask of material.The hygenic towelette formula facial mask for wherein infiltrating shin moisturizer occupies on the market Very big share, face paper can be divided into silk, cotton, terylene, blended, biological fiber etc. by material, Biofibre facial mask paper Effect is best, and blended face paper is worst.The material of face paper will affect the comfort and effect that facial mask uses.Natural textile quality Face paper be that most popular material, synthetic fibers material are taken second place in recent years.Slightly thick, important face paper can absorb enough Moisturizing essence, it is more preferable to the package effect of skin " pressurization ".Biofibre facial mask paper effect is best, generally the beauty in profession Hold chief minister to use, because somewhat expensive, the more difficult product for bearing this price of common people.
Nostoc Multifunctional cotton can be used as a kind of pure-natural biological facial mask and be subject to development and application.The material can realize function Property nutrition skin-protection ingredient (predominantly nostoc phycobniliprotein) and biological mask carriers natural combination, dry product state can be permanent It saves, and easy to carry, when use adds a small amount of drinking water or purified water is impregnated, and is most importantly not added with any Preservative.
Cotton pads on existing market is usually the small cotton piece that 5-6 centimetres is about as made of cotton or paper pulp compacting, thick About 0.1-3 centimetres, the cotton pads price that supermarket is sold is generally all very durable in 5-6 member or so;Better brand will be tens Member or so, it is on sale in cosmetic counter.Many using the use of cotton pads, most important purposes is exactly to remove ornaments and formal dress, because of its matter Ground very soft makeup removing when be not easy face to injure, toner is poured on can remove adornment very clean above.Cotton pads It can also be used to make up, have secondary clean effect in face wiping toner with it, be the not omissible step of skin care.
The material of cotton pads can be divided into two kinds substantially: absorbent cotton and non-woven fabrics, both materials respectively have advantage and disadvantage, can be with It selects according to their own needs.Using absorbent cotton is more thick and solid as the cotton pads of material, sense of touch is soft and fine, needs when for smearing toner Amount it is relatively more, have the phenomenon that furfur sometimes, water absorbing force is weaker;Using non-woven fabrics as the cotton pads of material then relatively thin, sense of touch It is relatively thick, but since water absorbing force is strong, so the dosage of toner compares province.
Anti-inflammatory plaster in the market is mostly various plaster products, has multilayered structure, and internal layer is generally soaked with drug Abiotic material.
The current pure natural nostoc biological material facial mask of dry product state that there are no skin care ingredient and mask carriers combine;Not See the cotton pads for having pure natural nostoc biological material;It there are no the small patch of anti-inflammatory of the Multifunctional cotton class of nostoc organism material Piece, this Anti-inflammatory plaster are entirely pure-natural biological material, and itself is living rich in the biology with anti-inflammatory and nutrition skin-protection Property ingredient.
Summary of the invention
It is an object of the present invention to provide a kind of nostoc Multifunctional cottons and the preparation method and application thereof.The present invention has found one kind The method that nostoc Multifunctional cotton can be conveniently made, simple process, and also it is environmentally protective.
Technical solution of the present invention is as follows:
A kind of preparation method of nostoc Multifunctional cotton, including nostoc is subjected to tissue mashing, algae is made in ultrasonication Gained algae is starched casting mold, is protected from light freezing in -10 DEG C or less by slurry, dry to get.
Preferably, the nostoc is the nostoc after sphere state fresh goods nostoc frond either dry product rehydration, or It is the dry powder that nostoc dry product smashes it through >=80 mesh screens.
Preferably, it by nostoc dry product rehydration, increases weight after being absorbed water using purified water or distilled water immersion nostoc to it Up to 16-50 times, further preferably 30-40 times, then the nostoc frond after recovery is subjected to tissue mashing.
Pulverizer can be used in nostoc dry powder, and mistake >=80 mesh screens after crushing 3-8 minutes, the fine powder after taking sieving adds pure The rehydration ratio of change water or distilled water rehydration, nostoc dry powder and water is 1Kg:16L-1Kg:50L.
Generally, historrhexis's machine can be used to smash the nostoc as algae slurry, the performance of processing time view machine to pieces Depending on;Finally use colloid mill homogeneous.
Preferably, tissue mashing is using tissue mashing machine (preferably revolving speed 10-120r/min) first by frond tissue mashing;Again The tissue smashed to pieces is utilized into colloid mill (preferably revolving speed 1450-3200r/min processes 2-50 μm of fineness) Wet ultrafine grinding, benefit With shearing/grinding/high-speed stirred active force of colloid mill, the high force dispersion mixing of breaking-wall cell and algae slurry is realized.
Preferably, filtering is further included the steps that after tissue mashing.As needed, the sieve mistake of 80 mesh or more may be selected Algae slurry after filtering tissue mashing.It is smaller uniformly that experiment shows to select the sieve (preferably 80-150 mesh) of high mesh number that aperture can be obtained Fine and smooth Multifunctional cotton;The sieve (preferably 20-80 mesh) for selecting low mesh number, can be obtained the larger and coarse Multifunctional cotton in aperture. If without filtering, obtained Multifunctional cotton aperture is bigger and more coarse.
Preferably, the ultrasonication treatment conditions are frequency 20-25KHz, at interval of 10s ultrasound 5s, total ultrasound 5- 10min.Experiment shows to be more advantageous to breaking-wall cell under the conditions of the ultrasonic treatment, strengthens homogenizing effect.
If it is desired to preparing the Multifunctional cotton of face paper shape, then algae slurry is cast in face pattern tool, is poured thickness Can≤1cm, it is preferred with being poured thickness 0.3-0.5cm or so;If it is desired to being prepared into the shape of cotton pads, then algae is starched into being cast in In the mold of adornment cotton shape, casting thickness can≤2cm;If it is desired to being prepared into the small patch of anti-inflammatory skin care, then small-size flaky may be selected Mold, casting thickness can≤1cm;In short, can be as needed, select mold of different shapes to be poured.
Preferably, it is described be frozen in be protected from light or dark condition under carry out.
Be protected from light or dark condition under carry out freezing can effectively avoid phycobniliprotein meet photofading decompose.
Preferably, the cryogenic temperature is -10 DEG C or less.Generally, the temperature of general refrigerator, refrigerator-freezer or freezer is adjusted to This low temperature environment can be realized in freezing shelves;If the rate request to freezing is higher, liquid nitrogen freezing, which can be used, be can be realized fastly Quickly cooling is frozen.
Preferably, will gained nostoc Multifunctional cotton collect after be stored in be protected from light or dark dry environment in.
Specifically, the preparation method of above-mentioned nostoc Multifunctional cotton, comprising the following steps:
Nostoc is subjected to tissue mashing;Again by the tissue smashed to pieces using colloid mill (preferably revolving speed 1450-3200r/min, Process 2-50 μm of fineness) Wet ultrafine grinding;It is filtered using the sieve of 80-150 mesh;In frequency 20-25KHz, at interval of 10s ultrasound 5s carries out ultrasonication processing under the conditions of being total to ultrasound 5-10min;Algae slurry is made;Gained algae slurry is cast in face pattern In tool, it is poured thickness 0.3-0.5cm;
Or gained algae slurry is cast in the mold of cotton pads shape, it is poured thickness≤2cm;
Or gained algae slurry is cast in sheet mold, it is poured thickness≤1cm;
To pour and be poured in the mold of algae slurry and be placed in -10 DEG C or less and be protected from light freezing, dry to get.
The invention also includes the nostoc Multifunctional cottons of above method preparation;Its main nutrient composition is phycobniliprotein.
The invention also includes the nostoc Multifunctional cottons prepared according to the above method to be used to prepare facial mask, cotton pads or Anti-inflammatory plaster Deng application.Dry product state pure-natural biological material facial mask, the cotton pads of pure-natural biological material, pure day is for example made in the application Right biological material Anti-inflammatory plaster etc..
Nostoc Multifunctional cotton is porous spongy pure-natural biological material.After this material of water infiltration, poroid knot Structure will be filled by the protein liquid rich in phycobniliprotein.This protein crude extract rich in phycobniliprotein has significant anti-oxidant And the effect of anti-inflammatory, it is a kind of pure natural nutrition skin-protection liquid.And its cellular sponge structure is then a kind of pure natural skin care Carrier material.
Nostoc Multifunctional cotton is a kind of organism material with multiple functions, by first being smash to nostoc frond The working process freezed after broken changes the molecular composition pattern for smashing rear sticky and uniform algae slurry to pieces, forms A kind of porous spongy structure, the preparation process of this material do not add any chemical component, belong to pure-natural biological material.
The invention has the advantages that
(1) product processing thought is novel
It is freezed after breaking-wall cell, the gum components (mainly high molecular weight protein and macromolecular polysaccharide class) of nostoc do not dissolve in Water, and main function nutritional ingredient phycobniliprotein is dissolved in water, after entire biomaterial freezes, utilizes the water-soluble of phycobniliprotein Property, freeze phycobniliprotein together with water, at the same time, water-insoluble large biological molecule is close to each other, leads to adjacent macromolecular Chemical bond contact with each other to form new more stable combination, that is, the polymerization reaction between large biological molecule has occurred, simultaneously The separation for realizing phycobniliprotein Yu other water-insoluble large biological molecules recycles dry method to remove water, makes algae gallbladder Albumen has stayed in the hole where the ice crystal that original moisture content is formed, i.e. the cotton structural body of phycobniliprotein storage after the drying Hole in, therefore nostoc organism itself formed cotton material become as its own phycobniliprotein released Storage carrier, cotton material phycobniliprotein after with water infiltration will be released endlessly.
The method of the present invention technique is unique, without compacting, algae can be realized by freezing and starch adhesion into the mesh of the cotton scraps of paper 's.
(2) Product Safety is prominent
On the market without the face paper of pure plant material.Multifunctional cotton of the present invention is a kind of pure-natural biological material: Cotton material is the own biological body after nostoc is smashed to pieces;Phycobniliprotein is the natural pigment albumen of nostoc itself synthesis;? In manufacturing process, other than adding purified water, any chemical reagents are not added.Because cotton material belongs to dry product state, algae gallbladder Albumen is also dry product state, and phycobniliprotein is stored in the hole of dry product cotton, does not add any adhesive and preservative, makes Used time need to only use clean clear water infiltration, and security performance is high.
(3) product utility is prominent
It has been sufficiently reserved the bioactivity of nostoc phycobniliprotein.In Multifunctional cotton containing nutrition pure natural in algae at Point, it is most distinctive to be the water-soluble phycobniliprotein of high-content, as long as cotton piece is immersed in clean clear water, phycobniliprotein It releases, and there is anti-oxidant, anti-radiation, anti-aging, antibacterial anti-inflammatory, antiallergy and other effects, can effectively nutrition and clean Skin.
(4) product new style
Own bioactivity ingredient phycobniliprotein is stored in the cotton structure hole of itself formation, makes its bioactivity Stablize, effect protrudes, usage safety performance height.Nostoc Multifunctional cotton can play the effect of facial mask, and it can be used repeatedly, Due to being rich in phycobniliprotein class nutritional ingredient in its porous spongy biomaterial, will be discharged after adding clean clear water to be infiltrated Out, become the pure natural shin moisturizer for being conducive to skin health, spongelike structure then plays the effect of mask carriers.Therefore Nostoc Multifunctional cotton facial mask realizes the perfect combination of shin moisturizer and mask carriers, is a kind of dry product state pure-natural biological material Facial mask.
Nostoc Multifunctional cotton can also play the effect of cotton pads, not only environmentally protective, and use soft comfortable. The difference is that nostoc Multifunctional cotton is not necessarily to dip external source toner, toner or makeup remover, the cellular knot of its own Rich in functional components such as phycobniliprotein with significant anti-oxidant and anti-inflammatory effect in structure, when use, only needs plus a small amount of cleaning is drunk Water immersion can be such that the functional components such as phycobniliprotein dissolve out.In consideration of it, using nostoc Multifunctional cotton as cotton pads in use, The effect of skin care can also be played.
In addition, nostoc Multifunctional cotton, which is alternatively arranged as a kind of anti-inflammatory material, gives development and application, such as to be made into anti-inflammatory small Patch.This Anti-inflammatory plaster is complete pure-natural biological material, and itself is rich in the bioactive ingredients with anti-inflammatory effect.
The present invention has already passed through test of many times verifying, the results showed that first smashs into nostoc frond to pieces again that its is cold after algae is starched Freeze generation porous spongy structure that can actually be stable, and rich in the function based on phycobniliprotein in its vesicular texture Property nutritional ingredient, can be released with this protein crude extract after water infiltration.
Detailed description of the invention
Fig. 1 is 1 nostoc of experimental example figure compared with Nostoc commune, the microscopic appearance delivered vegetables.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..It is not specified in embodiment specific Technology or conditions person, described technology or conditions according to the literature in the art, or carried out according to product description.It is used Production firm person is not specified in reagent or instrument, is the conventional products that can be commercially available by regular distributor.
Embodiment 1
A kind of preparation method of nostoc Multifunctional cotton, comprising the following steps:
Using nostoc fresh goods as initial raw materials, nostoc fresh goods state spherical shape frond is subjected to tissue mashing, tissue It smashs to pieces using tissue mashing machine (revolving speed 80r/min) first by frond tissue mashing;The tissue smashed to pieces is utilized into colloid mill (revolving speed again 2800r/min processes 50 μm of fineness) Wet ultrafine grinding, utilize shearing/grinding/high-speed stirred active force of colloid mill, realization The high force dispersion mixing of breaking-wall cell and algae slurry;It is filtered using the sieve of 100 mesh;It is broken that ultrasound is carried out under the conditions of 25KHz Broken processing 5min (at interval of 10s ultrasound 5s);Algae slurry is made;Gained algae slurry is cast in face pattern tool respectively, is poured thickness 0.4cm;Gained algae slurry is cast in the mold of cotton pads shape, thickness 1cm is poured;Gained algae slurry is cast in sheet mold In, it is poured thickness 0.5cm;It will pour respectively and be poured in the mold of algae slurry and be placed in -10 DEG C and be protected from light freezing, be integrally placed at after thawing under room temperature Under the conditions of 35 DEG C of hot air circulations dry to get.
Embodiment 2
Using nostoc fresh goods as initial raw materials, nostoc fresh goods state spherical shape frond is subjected to tissue mashing, tissue It smashs to pieces using tissue mashing machine (revolving speed 80r/min) first by frond tissue mashing;The tissue smashed to pieces is utilized into colloid mill (revolving speed again 2800r/min processes 50 μm of fineness) Wet ultrafine grinding, utilize shearing/grinding/high-speed stirred active force of colloid mill, realization The high force dispersion mixing of breaking-wall cell and algae slurry;It is filtered using the sieve of 100 mesh;It is broken that ultrasound is carried out under the conditions of 25KHz Broken processing 5min (at interval of 10s ultrasound 5s);Algae slurry is made;Gained algae slurry is cast in face pattern tool respectively, is poured thickness 0.4cm;Gained algae slurry is cast in the mold of cotton pads shape, thickness 1cm is poured;Gained algae slurry is cast in sheet mold In, it is poured thickness 0.5cm;To pour respectively be poured in algae slurry mold be placed in vacuum freeze drier vacuum freeze-drying to get.
Embodiment 3
A kind of preparation method of nostoc Multifunctional cotton, comprising the following steps:
Using nostoc dry product as initial raw materials, by nostoc dry product rehydration, using purified water or distillation water logging Weight gain carries out tissue mashing up to 40 times, then by the nostoc frond after recovery after steeping nostoc to its water suction, and tissue mashing uses Tissue mashing machine (revolving speed 80r/min) is first by frond tissue mashing;The tissue smashed to pieces is utilized into colloid mill (revolving speed 2800r/ again Min processes 50 μm of fineness) Wet ultrafine grinding, using shearing/grinding/high-speed stirred active force of colloid mill, realization cell is broken The high force dispersion mixing of wall and algae slurry;It is filtered using the sieve of 100 mesh;Ultrasonication processing is carried out under the conditions of 25KHz 5min (at interval of 10s ultrasound 5s);Algae slurry is made;Gained algae slurry is cast in face pattern tool respectively, is poured thickness 0.4cm; Gained algae slurry is cast in the mold of cotton pads shape, thickness 1cm is poured;Gained algae slurry is cast in sheet mold, is poured Infuse thickness 0.5cm;The mold for being poured in algal filament will be poured respectively be placed in -10 DEG C and be protected from light freezing, be integrally placed at 35 DEG C after melting under room temperature Under the conditions of hot air circulation dry to get.
Embodiment 4
A kind of preparation method of nostoc Multifunctional cotton, comprising the following steps:
Using nostoc dry powder as initial feed form, the preparation process of nostoc dry powder are as follows: will first be done with pulverizer It savors nostoc to crush 5 minutes, after 80 mesh screens.Fine powder after taking sieving adds purified water or distilled water rehydration, and nostoc is dry The rehydration ratio of powder and water is 1Kg:32L.It is filtered using the sieve of 100 mesh;It is carried out at ultrasonication under the conditions of 25KHz It manages 5min (at interval of 10s ultrasound 5s);Algae slurry is made;Gained algae slurry is cast in face pattern tool respectively, is poured thickness 0.4cm;Gained algae slurry is cast in the mold of cotton pads shape, thickness 1cm is poured;Gained algae slurry is cast in sheet mold In, it is poured thickness 0.5cm;It will pour respectively and be poured in the mold of algae slurry and be placed in -10 DEG C and be protected from light freezing, be integrally placed at after thawing under room temperature Under the conditions of 35 DEG C of hot air circulations dry to get.
Comparative example 1
Using nostoc fresh goods as initial raw materials, nostoc fresh goods state spherical shape frond is subjected to tissue mashing, tissue It smashs to pieces using tissue mashing machine (revolving speed 80r/min) first by frond tissue mashing;The agar powder of addition 2% or 10% thereto Cassava modified starch or 5% guar gum or 2.5% carragheen or 3% sodium alginate or 20% gellan gum, then will Mixture using colloid mill (revolving speed 2800r/min, process 50 μm of fineness) Wet ultrafine grinding, using colloid mill shearing/grind Mill/high-speed stirred active force realizes the high force dispersion mixing of breaking-wall cell and algae slurry;It is filtered using the sieve of 100 mesh;? Ultrasonication processing 5min is carried out under the conditions of 25KHz (at interval of 10s ultrasound 5s);Algae slurry obtained does not have after being poured into mold There is into cotton characteristic.
Comparative example 2
Using nostoc dry powder as initial feed form, the preparation process of nostoc dry powder are as follows: first crush 3 with pulverizer After minute directly plus purified water or distilled water rehydration, the rehydration ratio of nostoc dry powder and water is 1Kg:32L.In 25KHz condition Lower progress ultrasonication handles 5min (at interval of 10s ultrasound 5s);Obtained algae slurry does not have after being poured into mold into cotton Characteristic.
Comparative example 3
With Nostoc commune (scientific name Nostoc commune also known as land for building dish, nostoc commune, lichens, Nostoc commune, land for building bacterium, Thunder God Bacterium, soft youngster, melon skin etc., be the combination of fungi and algae, belong to beads Cutleriales nostocaceae) be raw material, by 1 phase of embodiment Same method is operated.As a result poor at cotton characteristic, and stability is poor.
Comparative example 4
With (scientific name: Nostoc commune var.flagelliforme, different name Nostoc of delivering vegetables Flagelliforme claims hair-like nostoc, is a kind of algae of Cyanophyta nostoc purpose) it is raw material, it is identical by embodiment 1 Method is operated.It as a result cannot be at cotton.
Experimental example 1
Respectively to 1 nostoc of embodiment and 3 Nostoc commune of comparative example and comparative example 4 deliver vegetables at cotton characteristic carry out experiment grind Study carefully, (a. is nostoc-embodiment 1 to the result is shown in Figure 1;It b. is Nostoc commune-comparative example 3;It c. is-comparative example 4 of delivering vegetables).
1) nostoc is compared with the microscopic appearance of other two kinds of nostocs (Nostoc commune is delivered vegetables)
Fig. 1-a is the microscopic structure figure of the cotton structure of nostoc Multifunctional cotton made from 1 method of embodiment, cotton in figure The hole of a large amount of bubble formations is rich in plastid, hole size is more consistent, and is evenly distributed, while the whole matter of nostoc cotton Structure also shows uniform feature;Fig. 1-b is 3 gained Nostoc commune prepared product of comparative example, and it is tough can to form in a short time structure The very poor cotton object of property, also containing the hole of bubble formation in cotton body in figure, but hole difference in size is significant, quantity pole It is few, it is distributed extremely uneven;It is handled and is delivered vegetables using 4 method of comparative example, cotton body cannot be formed, Fig. 1-c is its microscopic structure. The micro-structure diagram of three kinds of nostocs shown in Fig. 1, sufficiently illustrating three kinds of nostocs, treated according to the method for the invention Texture difference, nostoc algae slurry have unique colloidal property before at cotton, and only nostoc is capable of forming stable quality High cotton.
2) nostoc and group's state seaweed comparison result
Seaweed, kelp, thallus laminariae are handled according to 1 method of embodiment respectively, these three seaweed all cannot be at cotton.
3) comparison result of nostoc and other unicellular alga plants other than nostoc
Because the gumminess of unicellular alga plant is substantially lower than nostoc, so being handled according to 4 method of embodiment slender Cotton structure cannot be all formed after the cell colony of born of the same parents algae.
4) comparison result of nostoc and Mycophyta fructification tremella, agaric
After handling Mycophyta fructification tremella and agaric using 1 method of embodiment, the formation of cotton structure is all had no.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.

Claims (10)

1. a kind of preparation method of nostoc Multifunctional cotton, which is characterized in that carry out tissue mashing including nostoc, ultrasound is broken It is broken, algae slurry is made, gained algae is starched into casting mold, is protected from light freezing in -10 DEG C or less, dry to get.
2. preparation method according to claim 1, which is characterized in that the nostoc is sphere state fresh goods either dry product Nostoc either nostoc dry product after rehydration smashes it through the nostoc dry powder of >=80 mesh screens.
3. preparation method according to claim 1 or 2, which is characterized in that the tissue mashing includes using tissue mashing Machine is first by frond tissue mashing;The tissue smashed to pieces is utilized into colloid mill Wet ultrafine grinding again.
4. preparation method according to claim 3, which is characterized in that tissue mashing machine revolving speed 10-120r/min;The glue Body grinds revolving speed 1450-3200r/min, processes 2-50 μm of fineness.
5. preparation method according to claim 3, which is characterized in that further include the steps that filtering after tissue mashing.
6. preparation method according to claim 5, which is characterized in that the mesh number of filter screen used is 80-150 mesh.
7. preparation method according to claim 1 or 2, which is characterized in that the ultrasonication condition is frequency 20- 25KHz is total to ultrasound 5-10min at interval of 10s ultrasound 5s.
8. preparation method according to claim 1 or 2, which comprises the following steps: nostoc is carried out tissue It smashs to pieces;It is filtered using the sieve of 80-150 mesh;In frequency 20-25KHz, ultrasound 5-10min item is total at interval of 10s ultrasound 5s Ultrasonication processing is carried out under part;Algae slurry is made;Gained algae slurry is cast in face pattern tool, thickness 0.3-0.5cm is poured;
Or gained algae slurry is cast in the mold of cotton pads shape, it is poured thickness≤2cm;
Or gained algae slurry is cast in sheet mold, it is poured thickness≤1cm;
To pour and be poured in the mold of algae slurry and be placed in -10 DEG C or less and be protected from light freezing, dry to get.
9. the nostoc Multifunctional cotton of any one of claim 1-8 the method preparation.
10. nostoc Multifunctional cotton described in claim 9 is used to prepare the application of facial mask, cotton pads or Anti-inflammatory plaster.
CN201610868282.6A 2016-09-29 2016-09-29 A kind of nostoc Multifunctional cotton and the preparation method and application thereof Active CN106344420B (en)

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