CN104055702B - A kind of skin antiallergic wetting agent - Google Patents
A kind of skin antiallergic wetting agent Download PDFInfo
- Publication number
- CN104055702B CN104055702B CN201410257045.7A CN201410257045A CN104055702B CN 104055702 B CN104055702 B CN 104055702B CN 201410257045 A CN201410257045 A CN 201410257045A CN 104055702 B CN104055702 B CN 104055702B
- Authority
- CN
- China
- Prior art keywords
- parts
- skin
- chinese medicine
- hyaluronic acid
- streptococcus zooepidemicus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of skin antiallergic wetting agent.The hyaluronic acid yield of the present invention is high, light transmittance is good, protein content is low, glucuronic acid content is high, soluble, moistening effect is good, the antiallergic skin moisturizer of the preparation good penetrability to skin, has the strongest antiallergic effect, safe without toxic side effect while the most effective moist dry skin.
Description
Technical field
The present invention relates to biological polyoses extract and applied technical field, particularly relate to a kind of skin antiallergic wetting agent.
Background technology
In daily life, people are frequently encountered by due to environment or climate reasons xerosis cutis situation therefore.Xerodermatic reason mainly has two: one to be dry, and relative humidity is low；Two are the normal water sorption of skin and barrier function likes infringement.Cause aging, environment and the injury of chemicals, the pathological changes of skin and the attribute of dry skin because have skin of the latter.Being the main uses of skin protection cosmetics for skin offer moisturizing, but for skin is than more sensitive crowd, the use of cosmetics can bring more skin problem, allergy is most common of which problem.
Allergic dermatitis is the skin allergy caused due to contact hypersensitivity antigen, the I allergic reaction type that it is mainly mediated by IgE.All have in heredity or the most susceptible people to specific antigen, when contacting this antigen, may result in anaphylactic type or delayed-type hypersensitivity dermatitis, be primarily referred to as human contact cause to some anaphylactogen red swelling of the skin, itch, welt, the skin disorder such as decortication.Needing again to make to apply some make up to carry out the crowd of skin moisture-keeping for easy allergy, it is necessary for researching and developing a kind of safe antianaphylactic wetting agent.
Hyaluronic acid, referred to as HA, be the acid mucopolysaccharide being formed by connecting by β-(1 → 3) and β-(1 → 4) glycosidic bond by glucuronic acid and acetylglucosamine.It has the advantageous properties such as humectant, viscoelasticity and biocompatibility, especially has the performance of keeping humidity of uniqueness, has been widely used in the fields such as medicine, daily use chemicals, food, has been one of domestic and international biochemical industry circle focus product.
The domestic manufacturer having tens hyaluronic acids at present, and these enterprise's great majority are to use animal tissue's extraction method.But owing to raw material is limited, and the content of HA is low in raw material, HA the most also coexists in biological tissue with the mucopolysaccharide such as chondroitin sulfate simultaneously, causes that its yield is low, purify difficulty, high cost, poor product quality, and is dfficult to apply to large-scale production.Along with the continuous increase of HA demand, an urgent demand produces substantial amounts of high-quality HA, animal tissue's extraction method oneself do not adapt to this requirement.Technique fermentation period length, product relative molecular weight that the most also Production by Microorganism Fermentation hyaluronic acid uses are low, product printing opacity rate variance, protein content high, glucuronic acid content is low.The form of some hyaluronic acids is threadiness, not readily dissolves.It is extremely difficult to be applied to the standard of cosmetic field.
Summary of the invention
The purpose of the present invention is aiming at the defect of above-mentioned existence and provides a kind of skin antiallergic wetting agent.The hyaluronic acid yield of the present invention is high, light transmittance is good, protein content is low, glucuronic acid content is high, soluble, moistening effect is good, the antiallergic skin moisturizer of the preparation good penetrability to skin, has the strongest antiallergic effect, safe without toxic side effect while the most effective moist dry skin.
A kind of skin antiallergic wetting agent technical scheme of the present invention is, this wetting agent contains hyaluronic acid and Chinese medicine extract, described hyaluronic acid use streptococcus zooepidemicus (Streptococcus
zooepidemicus) AWA008 ferments preparation, streptococcus zooepidemicus (Streptococcus
zooepidemicus) AWA008 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, deposit number is: CGMCCNo.9111, and preservation date is on 04 29th, 2014.
This streptococcus zooepidemicus is at the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode: 100101 at present, and its deposit number is: CGMCCNo.9111, and the Latin title of strain isStreptococcus
zooepidemicus, the microorganism (strain) of ginseng evidence: AWA008, preservation date is on 04 29th, 2014.
Streptococcus zooepidemicus strain characteristics of the present invention: spherical in shape or oval, in catenation, has pod membrane, without spore, atrichia, Gram-positive.
Described Chinese medicine extract is prepared from by following Chinese medicine: Folium Artemisiae Argyi, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Herba Portulacae, Flos Chrysanthemi, Radix Scutellariae, Cortex Phellodendri, Radix Glycyrrhizae.
Described Chinese medicine extract is prepared from by following Chinese medicine by weight: Folium Artemisiae Argyi 8-10 part, Radix Astragali 10-12 part, Rhizoma Atractylodis Macrocephalae 3-5 part, Herba Portulacae 3-5 part, Flos Chrysanthemi 5-10 part, Radix Scutellariae 5-10 part, Cortex Phellodendri 3-5 part, Radix Glycyrrhizae 4-8 part.
Preferably, described Chinese medicine extract is prepared from by following Chinese medicine by weight: Folium Artemisiae Argyi 10 parts, the Radix Astragali 12 parts, the Rhizoma Atractylodis Macrocephalae 4 parts, Herba Portulacae 3 parts, Flos Chrysanthemi 8 parts, Radix Scutellariae 6 parts, Cortex Phellodendri 4 parts, 8 parts of Radix Glycyrrhizae.
The preparation process of described Chinese medicine extract is: weighs Chinese medicine the most by weight and pulverizes, with water-ethanol as Extraction solvent, and heating and refluxing extraction three times, each two hours, united extraction liquid, discard medicinal residues, extracting solution concentrated recovery ethanol, transpiring moisture uses after being spray-dried and obtains Chinese medicine extract.
Described hyaluronic acid uses streptococcus zooepidemicus fermentation preparation to comprise the following steps:
(1) shake-flask seed liquid is cultivated: slant strains being inoculated in equipped with in the 1L conical flask of sterile medium, cultivate 12-16 hour for 37 DEG C, it is good that microscopy observes thalli growth, without living contaminants, can be inoculated in seed tank；
(2) strain expansion is joined: joined by culture fluid in seed tank, after carrying out steam sterilization, design temperature 36.3 DEG C, pH6.86, in seed tank, strain is added by sterility requirements, maintaining tank pressure 0.05MPa, cultivate 12-16 hour, it is good that microscopy observes thalli growth, without living contaminants, can be inoculated in fermentation tank；
(3) fermentation: half fermentation culture is joined in fermentation tank, after carrying out steam sterilization, design temperature 36.3 DEG C, pH6.86, maintain tank pressure 0.05MP, the culture medium of seed tank be all pressed in fermentation tank, record pH value, add second half fermentation culture when pH declines, keep indices, when fermentation liquid pH fall per minute is less than 0.01, terminate fermentation, detection fermentation broth viscosity, is pressed into fermentation liquid in settling tank；
(4) front precipitation: the most gently add the ethanol of 95% in settling tank, to producing flakes precipitation, controls, at 47-50 degree (alcoholic strengths when folding is to 20 DEG C), to collect precipitate；
(5) dissolve: with sodium chloride solution, precipitate is dissolved, regulate pH to 9.3-9.5, add EDTA, activated carbon, perlite, stir；
(6) filter: after the feed liquid press-in big plate-and-frame filtration of 1# one time after dissolving, filtrate adjusts pH9.3-9.5.After adding white kieselguhr and perlite, after stirring, the press-in big plate-and-frame filtration of 2# second time.Adding kieselguhr in two times filtrate, after stirring, after feed liquid beats circular clarifying by corrosion resistant plate, filter the 3rd time, clarity is more than 99.5%, and then filtrate passes through polypropylene filter element filtering to clean room settling tank；
(7) postprecipitation: feed liquid adjusts pH to 7.0-7.3, gently adds 95% ethanol to precipitating generation under stirring, control ethanol number of degrees 47-50 degree.After static 20-40 minute, supernatant is squeezed into waste alcohol tank；
(8) dehydration is centrifugal: with the pH value of 75%-80% dehydration of alcohol between 7.0-7.3 6-7 time, the most again with 90% dehydration of alcohol 2-3 time, after dehydration, alcoholic strength counts to more than 90%, static about 1 hour, beating supernatant, sampling is surveyed pH value, and is controlled between 7.0-7.3.It is centrifuged after stirring 15-20 minute；
(9) dry: the material being centrifuged being put into drying chamber, design temperature 30 DEG C, after evacuation is dried 3 hours, heats up 5 DEG C per hour, if stopping during temperature 60 DEG C, detecting moisture, moisture goes out tank after being less than 7%.
Culture fluid in step (1), (2), (3) includes glucose 3-5% by weight percentage, yeast powder 1.5-2%, Magnesium sulfate heptahydrate 1-2%, four water manganese sulfate 0.01-0.1%, potassium dihydrogen phosphate 0.1-0.5%, disodium hydrogen phosphate 0.5-0.8%, calcium carbonate 0.1-1%, zinc chloride 0.05-0.1%, and surplus is water.
Step 2. concentration of sodium chloride solution is 1-5%.
Step 2. in, add EDTA, activated carbon, perlite mass ratio are, 1:10-15:20-30.
Preferably, add EDTA, activated carbon, perlite mass ratio are, 1:11:25.
Step 3. in, white kieselguhr and perlitic mass ratio are 1:1-10.
Preferably, white kieselguhr and perlitic mass ratio are 1:1.5.
In step (6), filtrate passes through 0.22 micron polypropylene filter element filtering to clean room settling tank.
The invention have the benefit that the antiallergic skin moisturizer of the present invention good penetrability to skin, it is possible to the most effective dry skin that moistens, the most sticky.The ability of the sky hyaluronic acid holding moisture contained in the present invention is strong.There is high biological compatible, and other components matching, have moisturizing, soft skin, crease-resistant, brighten, salubrious effect.Chinese medicine extract in the present invention cooperates, and has the effect of obvious antiallergic, moisturizing, antioxidation, whitening and anti-aging.Folium Artemisiae Argyi therein, Radix Scutellariae, Cortex Phellodendri, Radix Glycyrrhizae extract all have good antiallergic effect.
Product feel of the present invention is the most smooth rapid osmotic can enter skin histology, makes skin have gloss and salubrious wettability effect.The infiltration of skin is quick, gives skin moisturizing sense.Product of the present invention can improve rapidly the moisture holding capacity of skin, the vigor of activated cell, has holding skin and moistens, smooth, prevents the effect of xerosis cutis and obfuscation.
The present invention uses complex mutation technology screening to go out the strain that hyaluronic acid productivity is high, combining with fermentation technology, and hyaluronic acid product output capacity is high, up to 7-9g/L.The hyaluronic acid that fermentation obtains is easily isolated purification, with short production cycle.Using interpolation activated carbon, perlite, white kieselguhr, EDTA to combine big plate-and-frame filtration technology and remove the impurity in hyaluronic acid, additive is common to be easy to get, and operates relatively easy, and impurity content is lower.Using 75-80% ethanol that material has carried out 6-7 dehydration, 90% ethanol carries out 2-3 dehydration to material.The most progressively being dehydrated the moisture not only decreased in hyaluronic acid, it is easy to be dried, and washed away a small amount of impurity of residual in material, protein content is lower, and product purity is high.After using ethanol to be repeatedly dehydrated when reaching more than 90%, can obtain powder amorphous precipitated, dried product dissolubility is good, and bulk density is moderate.Hyaluronic acid product quality meets the standard of cosmetic field.
Detailed description of the invention:
In order to be more fully understood that the present invention, describe technical scheme in detail with instantiation below, but the invention is not limited in this.
Embodiment 1
A kind of skin antiallergic wetting agent technical scheme of the present invention is, a kind of streptococcus zooepidemicus (Streptococcus
zooepidemicus) AWA008, it at the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, its deposit number is: CGMCCNo.9111, preservation date is on 04 29th, 2014.
This streptococcus zooepidemicus is at the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode: 100101 at present, and its deposit number is: CGMCCNo.9111, and the Latin title of strain isStreptococcus
zooepidemicus, the microorganism (strain) of ginseng evidence: AWA008, preservation date is on 04 29th, 2014.
Streptococcus zooepidemicus strain characteristics of the present invention: spherical in shape or oval, in catenation, has pod membrane, without spore, atrichia, Gram-positive.
Described streptococcus zooepidemicus prepares the production technology of hyaluronic acid, comprises the following steps:
(1) shake-flask seed liquid is cultivated: slant strains being inoculated in equipped with in the 1L conical flask of sterile medium, cultivate 12-16 hour for 37 DEG C, it is good that microscopy observes thalli growth, without living contaminants, can be inoculated in seed tank；
(2) strain expansion is joined: joined by culture fluid in seed tank, after carrying out steam sterilization, design temperature 36.3 DEG C, pH6.86, in seed tank, strain is added by sterility requirements, maintaining tank pressure 0.05MPa, cultivate 12-16 hour, it is good that microscopy observes thalli growth, without living contaminants, can be inoculated in fermentation tank；
(3) fermentation: half fermentation culture is joined in fermentation tank, after carrying out steam sterilization, design temperature 36.3 DEG C, pH6.86, maintain tank pressure 0.05MP, the culture medium of seed tank be all pressed in fermentation tank, record pH value, add second half fermentation culture when pH declines, keep indices, when fermentation liquid pH fall per minute is less than 0.01, terminate fermentation, detection fermentation broth viscosity, is pressed into fermentation liquid in settling tank；
(4) front precipitation: the most gently add the ethanol of 95% in settling tank, to producing flakes precipitation, controls, at 47-50 degree (alcoholic strengths when folding is to 20 DEG C), to collect precipitate；
(5) dissolve: with sodium chloride solution, precipitate is dissolved, regulate pH to 9.3-9.5, add EDTA, activated carbon, perlite, stir；
(6) filter: after the feed liquid press-in big plate-and-frame filtration of 1# one time after dissolving, filtrate adjusts pH9.3-9.5.After adding white kieselguhr and perlite, after stirring, the press-in big plate-and-frame filtration of 2# second time.Adding kieselguhr in two times filtrate, after stirring, after feed liquid beats circular clarifying by corrosion resistant plate, filter the 3rd time, clarity is more than 99.5%, and then filtrate passes through polypropylene filter element filtering to clean room settling tank；
(7) postprecipitation: feed liquid adjusts pH to 7.0-7.3, gently adds 95% ethanol to precipitating generation under stirring, control ethanol number of degrees 47-50 degree.After static 20-40 minute, supernatant is squeezed into waste alcohol tank；
(8) dehydration is centrifugal: with the pH value of 75%-80% dehydration of alcohol between 7.0-7.3 6-7 time, the most again with 90% dehydration of alcohol 2-3 time, after dehydration, alcoholic strength counts to more than 90%, static about 1 hour, beating supernatant, sampling is surveyed pH value, and is controlled between 7.0-7.3.It is centrifuged after stirring 15-20 minute；
(9) dry: the material being centrifuged being put into drying chamber, design temperature 30 DEG C, after evacuation is dried 3 hours, heats up 5 DEG C per hour, if stopping during temperature 60 DEG C, detecting moisture, moisture goes out tank after being less than 7%.
Culture fluid in step (1), (2), (3) includes glucose 3% by weight percentage, yeast powder 1.5%, Magnesium sulfate heptahydrate 1%, four water manganese sulfate 0.05%, potassium dihydrogen phosphate 0.3%, disodium hydrogen phosphate 0.8%, calcium carbonate 0.2%, zinc chloride 0.08%, and surplus is water.
Step (5) concentration of sodium chloride solution is 3%.
In step (5), add EDTA, activated carbon, perlite mass ratio are, 1:11:25.
In step (6), white kieselguhr and perlitic mass ratio are 1:1.5.
In step (6), filtrate passes through 0.22 micron polypropylene filter element filtering to clean room settling tank.
1. the present invention uses complex mutation technology screening to go out the strain that hyaluronic acid productivity is high, combining with fermentation technology, and hyaluronic acid product productivity reaches 9g/L.The hyaluronic acid that fermentation obtains is easily isolated purification, with short production cycle.
2. the present invention uses interpolation activated carbon, perlite, white kieselguhr, EDTA to combine big plate-and-frame filtration technology and removes the impurity in hyaluronic acid, additive is common to be easy to get, operate relatively easy, impurity content lower (protein≤0.1%, heavy metal≤20ppm, arsenic salt≤2ppm).
3. the present invention uses 75-80% ethanol that material has carried out 6-7 dehydration, and 90% ethanol carries out 2-3 dehydration to material.The most progressively being dehydrated the moisture not only decreased in hyaluronic acid, it is easy to be dried, and washed away a small amount of impurity of residual in material, protein content is lower, and product purity is high, can reach more than 99.0%.
4. after the present invention uses ethanol to be repeatedly dehydrated when reaching more than 90%, can obtain powder amorphous precipitated, dried product dissolubility is good, and dissolution velocity is fast, and degraded is slow, bulk density moderate (bulk density 0.4～0.6g/ml).
Production technology the most of the present invention is simple, and supplementary material is common to be easy to get, and produces easily controllable, and impurity content is lower, is suitable for industrialization large-scale production, and yield is high, and greatly reduces production cost.
Embodiment 2
A kind of skin antiallergic wetting agent of the present invention, containing hyaluronic acid and Chinese medicine extract, described hyaluronic acid use streptococcus zooepidemicus (Streptococcus
zooepidemicus) AWA008 ferments preparation, streptococcus zooepidemicus (Streptococcus
zooepidemicus) AWA008 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, deposit number is: CGMCCNo.9111, and preservation date is on 04 29th, 2014.
Described Chinese medicine extract is prepared from by following Chinese medicine by weight: Folium Artemisiae Argyi 10 parts, the Radix Astragali 12 parts, the Rhizoma Atractylodis Macrocephalae 4 parts, Herba Portulacae 3 parts, Flos Chrysanthemi 8 parts, Radix Scutellariae 6 parts, Cortex Phellodendri 4 parts, 8 parts of Radix Glycyrrhizae.
The preparation process of described Chinese medicine extract is: weighs Chinese medicine the most by weight and pulverizes, with water-ethanol as Extraction solvent, and heating and refluxing extraction three times, each two hours, united extraction liquid, discard medicinal residues, extracting solution concentrated recovery ethanol, transpiring moisture uses after being spray-dried and obtains Chinese medicine extract.
The antiallergic skin moisturizer of the present invention good penetrability to skin, it is possible to the most effective dry skin that moistens, the most sticky.The ability of the sky hyaluronic acid holding moisture contained in the present invention is strong.There is high biological compatible, and other components matching, have moisturizing, soft skin, crease-resistant, brighten, salubrious effect.Chinese medicine extract in the present invention cooperates, and has the effect of obvious antiallergic, moisturizing, antioxidation, whitening and anti-aging.Folium Artemisiae Argyi therein, Radix Scutellariae, Cortex Phellodendri, Radix Glycyrrhizae extract all have good antiallergic effect.
Product feel of the present invention is the most smooth rapid osmotic can enter skin histology, makes skin have gloss and salubrious wettability effect.The infiltration of skin is quick, gives skin moisturizing sense.Product of the present invention can improve rapidly the moisture holding capacity of skin, the vigor of activated cell, has holding skin and moistens, smooth, prevents the effect of xerosis cutis and obfuscation.
Embodiment 3
A kind of skin antiallergic wetting agent of the present invention contains hyaluronic acid and Chinese medicine extract, described hyaluronic acid use streptococcus zooepidemicus (Streptococcus
zooepidemicus) AWA008 ferments preparation, streptococcus zooepidemicus (Streptococcus
zooepidemicus) AWA008 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, deposit number is: CGMCCNo.9111, and preservation date is on 04 29th, 2014.
Described Chinese medicine extract is prepared from by following Chinese medicine by weight: Folium Artemisiae Argyi 8 parts, the Radix Astragali 10 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, Herba Portulacae 5 parts, Flos Chrysanthemi 5 parts, Radix Scutellariae 10 parts, Cortex Phellodendri 3 parts, 4 parts of Radix Glycyrrhizae.
The preparation process of described Chinese medicine extract is: weighs Chinese medicine the most by weight and pulverizes, with water-ethanol as Extraction solvent, and heating and refluxing extraction three times, each two hours, united extraction liquid, discard medicinal residues, extracting solution concentrated recovery ethanol, transpiring moisture uses after being spray-dried and obtains Chinese medicine extract.
The antiallergic skin moisturizer of the present invention good penetrability to skin, it is possible to the most effective dry skin that moistens, the most sticky.The ability of the sky hyaluronic acid holding moisture contained in the present invention is strong.There is high biological compatible, and other components matching, have moisturizing, soft skin, crease-resistant, brighten, salubrious effect.Chinese medicine extract in the present invention cooperates, and has the effect of obvious antiallergic, moisturizing, antioxidation, whitening and anti-aging.Folium Artemisiae Argyi therein, Radix Scutellariae, Cortex Phellodendri, Radix Glycyrrhizae extract all have good antiallergic effect.
Product feel of the present invention is the most smooth rapid osmotic can enter skin histology, makes skin have gloss and salubrious wettability effect.The infiltration of skin is quick, gives skin moisturizing sense.Product of the present invention can improve rapidly the moisture holding capacity of skin, the vigor of activated cell, has holding skin and moistens, smooth, prevents the effect of xerosis cutis and obfuscation.
Claims (1)
1. a skin antiallergic wetting agent, it is characterised in that this wetting agent contains hyaluronic acid and Chinese medicine extract, described hyaluronic acid use streptococcus zooepidemicus (Streptococcus zooepidemicus) AWA008 ferments preparation, streptococcus zooepidemicus (Streptococcus zooepidemicus) AWA008 deposit number is: CGMCCNo.9111, preservation date is on 04 29th, 2014；
Described Chinese medicine extract is prepared from by following Chinese medicine by weight: Folium Artemisiae Argyi 10 parts, the Radix Astragali 12 parts, the Rhizoma Atractylodis Macrocephalae 4 parts, Herba Portulacae 3 parts, Flos Chrysanthemi 8 parts, Radix Scutellariae 6 parts, Cortex Phellodendri 4 parts, 8 parts of Radix Glycyrrhizae；
The preparation process of described Chinese medicine extract is: weighs Chinese medicine the most by weight and pulverizes, with water-ethanol as Extraction solvent, and heating and refluxing extraction three times, each two hours, united extraction liquid, discard medicinal residues, extracting solution concentrated recovery ethanol, transpiring moisture uses after being spray-dried and obtains Chinese medicine extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410257045.7A CN104055702B (en) | 2014-06-11 | 2014-06-11 | A kind of skin antiallergic wetting agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410257045.7A CN104055702B (en) | 2014-06-11 | 2014-06-11 | A kind of skin antiallergic wetting agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104055702A CN104055702A (en) | 2014-09-24 |
| CN104055702B true CN104055702B (en) | 2016-12-07 |
Family
ID=51543825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410257045.7A Active CN104055702B (en) | 2014-06-11 | 2014-06-11 | A kind of skin antiallergic wetting agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104055702B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814916A (en) * | 2015-04-24 | 2015-08-05 | 梁珍惠 | Moisturizing essence and preparing method thereof |
| CN105342947A (en) * | 2015-11-12 | 2016-02-24 | 金林墨 | Skin moisturizer and preparation method thereof |
| CN105434288B (en) * | 2016-01-06 | 2019-09-17 | 广州熙然化妆品有限公司 | Plant essence liquid and its preparation method and application with skin whitening effect |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914594A (en) * | 2010-08-13 | 2010-12-15 | 武汉嘉发胶原蛋白研究所 | Biological fermentation extracting method for hyaluronic acid |
| CN102772644A (en) * | 2012-02-09 | 2012-11-14 | 北京工商大学 | Traditional Chinese medicine composition and preparation with detumescence repairing function and preparation method thereof |
| CN103127269A (en) * | 2011-12-05 | 2013-06-05 | 上海原尚草化妆品有限公司 | Extractive of Chinese herbal compound and cosmetics including active ingredients |
| CN103550093A (en) * | 2013-10-30 | 2014-02-05 | 青岛叁鼎卫生制品有限公司 | Faint scent shower gel |
-
2014
- 2014-06-11 CN CN201410257045.7A patent/CN104055702B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914594A (en) * | 2010-08-13 | 2010-12-15 | 武汉嘉发胶原蛋白研究所 | Biological fermentation extracting method for hyaluronic acid |
| CN103127269A (en) * | 2011-12-05 | 2013-06-05 | 上海原尚草化妆品有限公司 | Extractive of Chinese herbal compound and cosmetics including active ingredients |
| CN102772644A (en) * | 2012-02-09 | 2012-11-14 | 北京工商大学 | Traditional Chinese medicine composition and preparation with detumescence repairing function and preparation method thereof |
| CN103550093A (en) * | 2013-10-30 | 2014-02-05 | 青岛叁鼎卫生制品有限公司 | Faint scent shower gel |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104055702A (en) | 2014-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100402555C (en) | White fungus heteropolysaccharide and its extract, preparation method and uses | |
| CN108823261B (en) | Ultralow-molecular-weight dendrobium officinale polysaccharide and preparation and application thereof | |
| Liu et al. | Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds | |
| CN102936292B (en) | Preparation method of lycium barbarum polysaccharide having high antioxidant activity | |
| CN104672339B (en) | Cordyceps cicadae rhzomorph as well as preparation method and application thereof | |
| CN101412703A (en) | Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid | |
| CN102242165A (en) | Method for producing high molecular weight sodium hyaluronate through fermentation and culture medium utilized by same | |
| CN107312807A (en) | The enzymolysis preparation of the brown alga function oligosaccharides in one main laminaria source | |
| CN104498564A (en) | Low molecular weight chondroitin sulfate preparation method | |
| CN104055702B (en) | A kind of skin antiallergic wetting agent | |
| CN105603029A (en) | Extraction method of active walnut peptides | |
| CN107080778A (en) | A kind of multiplex-enzyme extraction technique of longan pulp solid carbon dioxide insoluble active thing and application | |
| CN106349398A (en) | Cichorium intybus inulin extracting method | |
| WO2022067774A1 (en) | Preparation method and application of sea cucumber polysaccharide | |
| CN107286264A (en) | The deep working method of Chinese date nutrient material separation | |
| CN101899120B (en) | Method for refining jujube polysaccharide | |
| CN104059865B (en) | A kind of streptococcus zooepidemicus and prepare the production technology of hyaluronic acid with it | |
| CN110256598A (en) | A kind of preparation method of difference bioactivity lentinan | |
| CN104055686B (en) | A kind of skin moisturizer | |
| CN102617893A (en) | Preparation and application of composition of panaxoside Rg3, ginseng polysaccharide and ganoderan | |
| CN104774827A (en) | Method for preparing alginate lyase from abalone internal organs | |
| CN102304191B (en) | Method for extracting crude polysaccharide from waste internal organs of sea worms | |
| CN100358921C (en) | Method for producing Konjac Glucomannan in high purity | |
| CN104055701B (en) | Anti-allergy conditioning shampoo | |
| CN104069019A (en) | Shampoo containing hyaluronic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 256600 No. twelve, No. 488, the Yellow River Road, Shandong, Binzhou Applicant after: AWA BIOTECH BINZHOU CO., LTD. Address before: 256600 the Yellow River twelve, Shandong, Binzhou, Bohai, twenty-four West Road head Applicant before: Binzhou An Hua biological engineering company limited |
|
| COR | Change of bibliographic data | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |