CN103127269A - Extractive of Chinese herbal compound and cosmetics including active ingredients - Google Patents
Extractive of Chinese herbal compound and cosmetics including active ingredients Download PDFInfo
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Abstract
The invention relates to an extracting complex of a Chinese herbal compound-'screen sensitization-resisting formula 'and cosmetics including the 'screen sensitization-resisting formula' as active ingredients. The 'screen sensitization-resisting' formula comprises nine kinds of traditional Chinese medicine of astragalus mongholicus, bighead atractylodes rhizome, saposhnikovia divaricata, chamomile, purslane, scutellaria baicalensis, radix sophorae flavescentis, smoked plum and row liquorice, has obvious functions of sensitization prevention, moisture retention and antioxidation, and can be used for preparing skin-soothing facial cleanser, skin-soothing calm toner, skin-soothing moisture retention emulsion, skin-soothing calm skin cream, skin-soothing calm essence, skin-soothing calm sleeping masks, skin-soothing mask pasting, skin-soothing calm shower gel and the like which have the above functions. The Chinese herbal compound is less in untoward effects and high in safety to the skin. The cosmetics including the 'screen sensitization-resisting formula' have functions of sensitization prevention, moisture retention and aging prevention.
Description
Technical field
The present invention relates to the extraction complex of a first Chinese medicine compound---" screen is driven quick side " and contain described " screen is driven quick side " as the cosmetics of active component.
Background technology
Allergic dermatitis is the skin allergy that causes due to contact hypersensitivity antigen, and it is mainly the I allergic reaction type by the IgE mediation.All have the upper or people of susceptible constitutionally of heredity to specific antigen, when this antigen of contact, can cause anaphylactic type or delayed-type hypersensitivity dermatitis, mainly refer to human body touch some anaphylactogen and cause red swelling of the skin, itch, the skin disorders such as welt, decortication.The antiallergic agent that some are common such as chlorphenamine, diphenhydramine have obvious sedation and nervus centralis untoward reaction, and in addition, this type of medicine also has anticholinergic effect, can cause the symptoms such as xerostomia, eye are done, blurred vision, constipation, urine retention; Recent research finds that astemizole (hismanal) and teldane (terfenadine) may cause rare, serious cardiac toxicity, can cause fatal arrhythmia; Other medicines: as hydrocortisone, triamcinolone acetonide of belonging to glucocorticoid etc. only be for external application, and mainly is applicable to allergic dermatitis, seborrheic dermatitis and pruritus etc., this class medicine is not answered prolonged application.Chinese medicine extract with anti-allergic effects becomes one of trend of antiallergic functional components development because of its natural low toxicity, non-evident effect, the application in practice that some Chinese medicine compound were gone through thousand especially confirms it is safely and effectively.
The imperial quick former side of screen is from " studying carefully former side ", and record is from " Yifangleiju ".We select the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae, Flos Chrysanthemi, Herba Portulacae, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Mume, Radix Glycyrrhizae nine flavor books on Chinese herbal medicine, and compatibility forms meticulously, and makes through special extraction process.
Composition principle: the herbal combination principle that this compound recipe is followed monarch, minister, helped, makes, for quick property skin quality problem, taked the reason skin scheme of " anti-fixed closing "." interior warp " meaning: " institute of heresy gathers, and its gas must be empty ".The skin allergy person how by positive QI-insufficiency, can not resist exopathogen, and exopathogen (anaphylactogen) is taken advantage of a weak point, and sends out to be skin allergy.The imperial quick side's of screen the Radix Astragali, sweet in the mouth is warm in nature, is longer than invigorating QI to consolidate the body surface resistance, and Shennong's Herbal is called its " main strong wind ", can tonification healthy energy, can fill again skin, real space between skin and muscles and dispel the wind and go out, therefore as monarch drug.The Rhizoma Atractylodis Macrocephalae is sweet, bitter, warm, the function strengthening superficial resistance to stop perspiration, and the spleen strengthening and damp drying, " Bencao Congxin volume one ": the Rhizoma Atractylodis Macrocephalae " highly seasoned gold paste, virtue exceedes good wine, hundred is evil outer imperial, fills in six internal organs ", assist the Radix Astragali strong in interior QI invigorating, consolidating superficial resistance is imperial evil outside; The Flos Chrysanthemi light weight rises floating, can evacuate flesh table ailment said due to cold or exposure, in " Medical Treasures of the Golden Chamber " " Hou Shi is black loose " take this medicine as main, the treatment strong wind, therefore that it can assist the Radix Astragali to dispel the wind is loose evil, the Rhizoma Atractylodis Macrocephalae, the medicinal ministerial drug of doing of Flos Chrysanthemi two.Radix Saposhnikoviae is hot, sweet, tepor, disperses ailment said due to cold or exposure, is the general medicine that dispels the wind, and matches with the Radix Astragali, and the Radix Astragali gets Radix Saposhnikoviae, and its merit is larger, and Radix Saposhnikoviae gets the Radix Astragali, can lead vital qi circumfluence whole body to go out by heresy; Fructus Mume the flowers are in blossom midwinter becomes midsummer in fact, reports spring-QI to foot, and spring is at five elements Ying Feng, should acid at the five tastes, therefore Fructus Mume sour in the mouth and can dispelling wind for relieving itching; Pain is that for cloudy itching sun, skin pruritus mostly are pathogenic heat for suffering from, and Herba Portulacae acid is cold, the heat-clearing and toxic substances removing removing heat from blood; The Radix Sophorae Flavescentis bitter cold, clearing away heat-damp and promoting diuresis, wind dispelling insecticide are antipruritic, and being used for alleviating skin allergy has the merit of taking stopgap measures, therefore Radix Saposhnikoviae, Fructus Mume, Herba Portulacae, Radix Sophorae Flavescentis are adjuvant drug.Radix Glycyrrhizae is sweet flat, heat-clearing and toxic substances removing, and the mediation property of medicine is as making medicine.The complete square invigorating QI to consolidate the body surface resistance that gathers altogether, the effect of dispelling wind for relieving itching be can yet be regarded as and treated the efficacious prescriptions of skin allergy.
Compatibility characteristic: the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae three flavor concerted effort consolidating superficial resistance, Fructus Mume, Herba Portulacae, Radix Scutellariae, the Radix Sophorae Flavescentis four flavors quiet skin of going all out, " outer consolidating superficial resistance " arranges in pairs or groups with the perfection of " interior quiet skin ", consists of tight flesh table " screen " one, can increase the skin defense function in the easy quick skin of calmness.The envoy Radix Glycyrrhizae, in medicine " state is old ", imperial quick while, mediation is complete square, and association can do altogether.Loose and use are mended by full side, and specimen is double to be reached, and make skin " lean on as screen, precious as beautiful " after use, enjoy releive, peacefulness.
Therefore, one of the present invention to theme as a kind of be the Chinese herbal medicine extracting complex of raw material by the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae, Flos Chrysanthemi, Herba Portulacae, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Mume, Radix Glycyrrhizae.
An object of the present invention is to provide the safe effective ingredient that extracts with special process as the Chinese medicine extraction complex from specific Chinese herbal medicine.Described complex has antiallergic, moisturizing and anti-oxidation efficacy.Another theme of the present invention comprises that also this Chinese medicine extraction complex has application in the cosmetics of antiallergic, moisturizing and antioxidation in preparation.
Therefore the present invention also comprises a kind of cosmetic composition, it is characterized in that containing aforesaid Chinese medicine extraction complex, and its Chinese medicine extraction complex is 1～20%, based on the gross weight of cosmetic complex.
According to the use position of cosmetics and the different requirements of function, the cosmetic composition that contains the Chinese medicine composite extract of this application can be the form of facial milk cleanser, surfactant, skin-care milk curd, protective skin cream, facial treatment essence liquid, facial film, mask, bath gel.According to the different demands of outward appearance, cosmetic composition of the present invention can be the forms such as emulsion, water preparation, cream frost, gel, pad pasting, lotion.
Cosmetic composition of the present invention can contain other beneficiating ingredient, described beneficiating ingredient is selected from: water, emollient, wetting agent, surfactant, emulsifying agent, antioxidant, aromatic, thickening agent, nertralizer, screening agent, vitamin and antiseptic, and their mixture.
Summary of the invention
The present invention realizes by following design in order to obtain having the Chinese medicine extraction complex with antiallergic, moisturizing and anti-oxidation efficacy of above-mentioned purpose:
Chinese medicine extraction complex of the present invention by comprise the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae, Flos Chrysanthemi, Herba Portulacae, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Mume, Radix Glycyrrhizae totally nine the flavor Chinese herbal medicine be prepared from, each composition percentage by weight is 19.39～21.42%, 11.63～12.86%, 11.63～12.86%, 5.82～6.43%, 11.63～12.86%, 11.63～12.86%, 5.82～6.43%, 5.82～6.43%, 11.63～12.86%.
The effect of material characteristics contained according to above-mentioned Chinese medicine itself and Chinese herbal compounds, Chinese medicine extraction complex of the present invention makes (Fig. 1) by ad hoc approach:
1. the Rhizoma Atractylodis Macrocephalae is given as one thinks fit cataclasmly, adopts the light oil extractor, adds 10～20 times of weight pure water, extracts volatile oil with steam distillation, and extraction time is 5 hours, places a moment after stopped heating, collects volatile oil 1.
2. the Radix Astragali, Radix Saposhnikoviae, Flos Chrysanthemi, Herba Portulacae, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Mume, Radix Glycyrrhizae are given as one thinks fit cataclasmly, with 50～70% ethanol extractions twice, extract 2 hours with the solvent of 15 times of weight for the first time, for the second time with the solvent extraction of 10 times of weight 2 hours.Merge extractive liquid, reclaims ethanol, filters, and is concentrated, and with aqueous dispersion, upper D101 type macroporous resin with the ethanol elution of water and variable concentrations gradient, is collected 30～70% ethanol elution respectively, reclaims solvent, obtains effective ingredient 2.
3. get appropriate volatile oil 1 and effective ingredient 2, be dissolved in (propylene glycol: water=1: 5～5: 5 (volume ratio)) in the aqueous solution of propylene glycol according to the ratio of 1: 50～1: 200 (weight ratio), obtain rufous and be clear to translucent liquid, be i.e. Chinese medicine compound of the present invention " screen is driven quick side " extract.
Chinese medicine compound of the present invention " screen is driven quick side " extract PH scope: 4.5～6.5; Detect through thin layer chromatography, its main chemical compositions has: flavone, polysaccharide, volatile oil and glycoside.
Effective ingredient is described:
One. safety
1. skin irritation
Because active component of the present invention is to add in the cosmetics that directly are applied in skin, it is most important whether it produces zest to skin.Holder Shanghai Institute of Pharmaceutical Industry of special commission has carried out repeatedly the skin irritation test.
1.1. material and object
Material: Chinese medicine compound " screen is driven quick side " extract
Animal: Cavia porcellus (male and female are not limit), 250g～300g.
Occupancy permit number: SYXK (Shanghai) 2009-0068
Production licence number: SCXK (Shanghai) 2007-0008
Experiment condition: 20～24 ℃ of laboratory temperatures, relative humidity 60～70%.
1.2. method
" cosmetics health standard " (2007) of issuing with reference to Ministry of Public Health.
1.3. result
Report sees Fig. 2 for details, and result shows: the experiment through 14 days, the irritant reaction integration is 0, shows that " screen is driven quick side " composite extract is non-stimulated to skin.
2. patch test
By patch test, detect the Potential feasibility that tested material causes the human body skin untoward reaction." cosmetics health standard " (2007) that method is issued with reference to Ministry of Public Health.
2.1. material and object
Be subjected to test product: 1: the peace skin sleep facial film of releiving; 2: peace skin moisturizing curdled milk; 3: the peace skin essence of releiving.More than be subjected to contain in test product " screen is driven quick side " composite extract 20%.Blank: do not smear any product.
The experimenter: totally 30 people, male 10 people, female 20 people at 22～59 years old age, meet the experimenter and volunteer inclusion criteria.
2.2. speckle method for testing
Select qualified speckle examination device, with closed patch test method, with tested material approximately 0.020～0.025ml be applied in speckle examination device, the external special adhesive tape is affixed on the experimenter back, remove tested material after 24 hours, respectively at 0.5,24,48 hour observation dermoreaction after removing, record its result by dermoreaction grade scale in " cosmetics health standard " (version in 2007).
2.3. result
Explain " occurring the number of I level (suspicious reaction) untoward reaction in 30 routine experimenters more than 5 examples; or 2 grades of untoward reaction numbers are more than 2 examples; or any 1 example occurs more than 3 grades or 3 grades during skin adverse reaction; judge that tested material has untoward reaction to human body " according to the enclosed type patch test results, the test product that is subjected to that my company is used for patch test is negative reaction (table 1), is subjected to test product all human body not to be had untoward reaction more than explanation.
Table 1 contains the human body patch test results of the imperial quick side's composite extract cosmetics of screen
Two effectiveness
In order to verify that Chinese medicine compound of the present invention " screen is driven quick side " extract has antiallergic, moisturizing and anti-oxidation efficacy, implemented following external and human experimentation:
1. test based on RBL-2H3 cell anti-hypersensitivity of type I
Experimental principle: the RBL-2H3 cell is the strain of Wistar rat basophilic leukemia cell, cell surface expression IgE high-affinity receptor, when being subject to the stimulation of antigen or non-antigenic substance, can discharge the granule of materials such as containing histamine, beta-amino hexose glycosidase, thereby produce a series of biological effect, now be widely used in correlational study as cell model.β-aminohexose glycosidase is a kind of in the granule lysosomal enzyme that discharges of mastocyte, is the specific proteins of labelling mast cell degranulation, release parallel with histamine.Estimate the effect of medicine anti-hypersensitivity of type I by the burst size that detects histamine, beta-amino hexose glycosidase.
1.1. materials and methods
Instrument: cell culture super-clean bench, enzyme-linked immunosorbent assay instrument (biotek)
Reagent: DMEM culture medium, IgE (Anti-DNP IgE) (sigma), p-Nitrophenyl-N-Acetyl-β-D-Glucosaminide (PNAG) (Sigma), dinitrophenylation human albumin (DNP-HSA) (sigma), 96 porocyte culture plates (coster)
Cell culture: the RBL-2H3 cell is 10% FBS (Ilex purpurea Hassk.[I.chinensis Sims) containing volume fraction, penicillin 1 * 10
5UL-1 and streptomycin 1 * 10
5In the DMEM culture medium of UL-1,37 ℃, 5%CO
2Cultivate under condition.2～3d changes liquid and goes down to posterity 1 time.
The RBL-2H3 cell activation takes off granule:
The RBL-2H3 cell is washed 2 times with PBS, with 2-3 * 10
5On density inoculation 96 well culture plates of cells/ml, every hole 180 μ l.Grouping: normal cell group, negative control group, anaphylaxis group.Establish 6 parallel holes for every group, 37 ℃ of overnight incubation.The every hole of anaphylaxis group and negative control group adds 20 μ l DNP-IgE sensitization, cultivates 24h in incubator.
PBS washes plate 2 times, washing liquid adds 80 μ l basic culture solutions after all exhausting successively, and normal group adds 100 μ l basic culture solutions, the medicine that adds 10ul to the anaphylaxis group, drug level are respectively 25,5,1,0.2,0.04,0.008,0.0016,0.00032 μ g/ml; Negative control group adds 10 μ lPBS, and after hatching 0.5h, it is the DNP-HSA of 1000 μ g/l that anaphylaxis group and negative control group add 10 μ l final concentrations, after bringing out antigen antibody reaction 0.5h, and ice bath 10min cessation reaction.
The mensuration of beta-amino hexose glycosidase burst size:
Transferase 45 0 μ l supernatant to 96 orifice plate, the citric acid soln that adds 50 μ l 1mmol/l-1PNAG, rock gently and make mixing, 37 ℃ of reaction 1.5h, the beta-amino hexose glycosidase that the cell degranulation reaction discharges can be hydrolyzed to paranitrophenol with PNAG, add 200 μ l carbonic acid buffer colour developings, measure wavelength 405nm, measure each hole absorbance on enzyme-linked immunosorbent assay instrument.Suppress beta-amino hexose glycosidase release rate (%)=[(anaphylaxis group absorbance-blank group absorbance)/(negative group absorbance-blank group absorbance)] * 100%.
The mensuration of histamine release amount:
Getting cell conditioned medium liquid 50 μ l detects with rat histamine (HIS) enzyme-linked immunoassay kit.
Experimental principle: adopt the enzyme linked immunological competition law to detect HIS content.At first with the coated microwell plate of goat-anti rabbit, be prepared into solid phase two anti-, then add testing sample, horseradish peroxidase-labeled HIS and anti-HIS antibody, make it to form coated two anti--anti-HIS antibody-HIS (HRP) complex, the binding capacity of labelling HIS and the amount of the HIS in sample are inversely proportional to.Measure absorbance (OD value) on microplate reader after colour developing, mapping match concentration-absorbance curve, inverse goes out HIS content in specimen to be measured.
Operating procedure: various reagent are put equilibrium at room temperature half an hour, get concentrated cleaning solution, according to when batch amount detection, with dilution in 1: 20, standby after mixing with distilled water.ELISA Plate is taken out, establish a blank hole, do not add any liquid; Each standard point is respectively established two holes successively, and every hole adds respective standard product 50 μ l, and standard substance concentration is respectively 0.16,0.62,2.5,10,40ng/ml; All the other every holes directly add testing sample 50 μ l, and sample concentration is respectively 25,5,1,0.2,0.04,0.008,0.0016,0.00032 μ g/ml; Every hole adds enzyme conjugates 50 μ l (blank hole except), then adds antibody 50 μ l by same order, and fully mixing, stick the adhesive sticker mounting, put 37 ℃ of incubations 1 hour.
Wash plate by hand, discard liquid in the hole.Cleaning mixture is filled with each hole, dries, and pats dry after triplicate in standing 10 seconds.Every hole adds colour developing A liquid 50 μ l, colour developing B liquid 50 μ l, and after the vibration mixing, 37 ℃ of lucifuges developed the color 15 minutes, and every hole adds stop buffer 50 μ l.The microplate reader reading is measured the absorbance under wavelength 450nm.
Statistical method: experimental data all represents with x ± s, and many groups are relatively adopted one factor analysis of variance, relatively adopts the t check between two groups, uses the SPSS16.0 statistical software to analyze.
1.2. result and discussion
1.2.1. the imperial quick formula extraction of screen discharges the inhibitory action of beta-amino hexose glycosidase to the RBL-2H3 cell
From table 2, Fig. 3 as seen, the anaphylaxis group is in dosage is 1.6ng/ml～25 μ g/ml scopes, show the effect (suppression ratio＞80%) that good inhibition beta-amino hexose glycosidase discharges, compare with the feminine gender group, difference is (P＜0.01) extremely significantly.Along with the reduction of dosage, suppression ratio is downward trend gradually, demonstrates good dose-effect relationship.And when dosage was 0.32ng/ml, its suppression ratio illustrated that still greater than 50% this extract has good inhibitory action to the release of beta-amino hexose glycosidase.
The imperial quick formula extraction of table 2 screen discharges the inhibitory action (n=6) of beta-amino hexose glycosidase to the RBL-2H3 cell
Annotate: compare * * P＜0.01, * P＜0.05 with the feminine gender group.
1.2.2. the imperial quick formula extraction of screen discharges the inhibitory action of histamine to the RBL-2H3 cell
Adopt the enzyme linked immunological competition law to detect HIS content, drawing histamine release quantitative determination standard curve is y=-0.0164x+0.7087 (r=0.9982), calculate according to this burst size of each group histamine, and then draw the suppression ratio that under different dosing dosage, the RBL-2H3 cell is discharged histamine.Experimental result such as table 3, shown in Figure 4.Along with the reduction of dosage, suppression ratio is downward trend gradually, demonstrates good dose-effect relationship.With the feminine gender group relatively, dosage is when 25 μ g/ml～8ng/ml, difference is (P＜0.01) extremely significantly, when dosage is 0.32ng/ml, significant difference (P＜0.05).Wherein, suppression ratio is up to 98.32%.
The imperial quick formula extraction of table 3 screen discharges the inhibitory action (n=6) of histamine to the RBL-2H3 cell
Annotate: compare * * P＜0.01, * P＜0.05 with the feminine gender group.
2. sensitivity skin sensation of pricking experiment
By causing the power of the subjective sensations such as twinge after the facial proof test thing of the experimenter with sensitivity skin and reference substance, the test tested material is to sensitive-skinned subjective irritation.Special commission's holder Shanghai City Hospital of Skin Diseases carries out the sensation of pricking experiment.
2.1. materials and methods
Given the test agent: the 1. peace skin essence (accept numbering CS-2010-25) of releiving; 2. pacify skin moisturizing curdled milk (accepting numbering CS-2010-26); More than be subjected to contain in test product " screen is driven quick side " composite extract 20%.Positive control: 8% lactic acid; Negative control: distilled water.
The experimenter: 30 healthy adult women, 30～60 years old age, be responsive skin, sensation of pricking and clear expression can be felt without any dermatopathy in tested position.
Test method: test divides to be carried out in two weeks.Carry out sample and positive reference substance twinge test the first week, second week carries out sample and negative control product twinge test.The experimenter dries with dried and soft medicated napkin and cleans position, specimen and reference substance after sitting quietly 10 minutes with clear water cleaning bilateral nasolabial fold position.Sample and reference substance are by the random number sequence.Use adjustable pipette to draw 0.05ml sample and reference substance, the experimenter is applied in tested position 1cm with finger with sample
2, smear evenly as far as possible.Reference substance drips the filter paper in diameter 8mm, is placed in to be subjected to test product offside nasolabial fold.Position pruritus, twinge, three kinds of uncomfortable degree of burn feeling are tested in assessment in 20～30 seconds, 2.5,5,8 minutes: 0 is without sense of discomfort; 1 is slight uncomfortable; 2 is that moderate is uncomfortable; 3 is that severe is uncomfortable.After assessment finishes, wipe with medicated napkin and test the position product.
2.2. statistical method is with Wilcoxon and X
2Check.Significance level is P＜0.05.
2.3. experimental result
The given the test agent test result is seen Fig. 5,6,7,8.Tested material is through responsive skin test, and the total points of its scratchiness, sensation of pricking, the various sensations of burn feeling all is starkly lower than lactic acid, and both have significant difference; Tested material is through the test of responsive skin, and the total points of its scratchiness, sensation of pricking, the various sensations of burn feeling is compared there was no significant difference with distilled water, illustrates that it can effectively pacify responsive skin, has no side effect, and can satisfy responsive skin crowd to the demand of skin care item.
3. moistening effect checking
3.1. external moisturizing test
3.1.1. experiment material: screen is driven quick formula extraction; 10% glycerol; Medical adhesive tape.
3.1.2. test method: weight-loss method is adopted in experiment, as carrier, various samples is spread upon tape surface with medical adhesive tape, is placed in respectively the environment of humidity 81% and 43%, room temperature 18 degree.Divide and weigh at 4h, 6h and 24h, calculate the moisturizing rate by following formula: moisturizing rate=(Wt-W)/(W-W
0), wherein Wt is for placing the weight after certain hour, and W is the weight that adds after sample, W
0Be the weight of application of sample not.
3.1.3 experimental result: as shown in table 4, under 81% ambient humidity, the moisturizing rate of the imperial quick formula extraction of screen is all far away higher than 10% glycerol.Only after 43% ambient humidity was placed 24h, the moisturizing rate of the imperial quick extract of screen descended to some extent, but its moistening effect is still suitable with 10% glycerol.
Table 4 screen is driven the external moistening effect of quick formula extraction (n=3)
3.2. human body moistening effect test and appraisal
3.2.1. experiment material: 1: peace skin moisturizing curdled milk; 2: the peace skin essence of releiving.More than be subjected to contain in test product " screen is driven quick side " composite extract 1%.
3.2.2. experimental technique: use clear water to clean volunteer's inboard arm, dry, every selected two zone (4cm of arm
2/ zone), and labelling, measure moisture and the TWEL value of respective regions skin with corneometer moisture of skin tester and moisture of skin loss (TEWL) tester, as the skin moisture content initial value, corresponding product is smeared respectively in each zone afterwards, skin moisture content and TWEL value after corresponding product 1h, 5h are smeared in test, and record data are done moisture of skin and the TWEL value changing trend diagram of test zone.The experimental situation temperature: 20 ± 1 ℃, humidity: 50%.
3.2.3. experimental result
The instant hydration meansigma methods of skin and skin hydration increment rate: skin hydration meansigma methods * 100% before skin hydration increment rate=(the front skin hydration meansigma methods of skin hydration meansigma methods-use product after the use product)/use product, result such as table 5, shown in Figure 7.After use essence and curdled milk one hour, skin moisture content all significantly increases than blank, and along with the prolongation of time, skin moisture content is progressively reducing, and after 5 hours, the hydration increment rate of peace skin moisturizing curdled milk becomes 33.69%.
TWEL meansigma methods and TWEL value rate of change: skin TEWL meansigma methods * 100% before TWEL value rate of change=(the front skin TEWL meansigma methods of skin TEWL meansigma methods-use product after the use product)/use product, result such as table 9, shown in Figure 10.Use peace skin moisturizing curdled milk after 1 hour and 5 hours, the TEWL value all reduces, and illustrates that curdled milk can strengthen the barrier function of skin.When using the peace skin to releive essence to 5 hour, the reduction of TEWL value.Illustrate the effect that prevents that moisture of skin runs off is both all arranged.
The instant hydration meansigma methods of table 5 skin and skin hydration increment rate (n=10)
The instant TWEL meansigma methods of table 6 skin and TWEL value rate of change (n=10)
4. antioxidation experiment
4.1. materials and methods
Experiment material and instrument: 1% screen is driven quick formula extraction; The 1%Vc ethylether; 1% licoflavone; 95% ethanol (Chemical Reagent Co., Ltd., Sinopharm Group); DPPH (Tokyo changes into Co., Ltd.); UV-1601 ultraviolet spectrophotometer (Japanese Shimadzu company).
Experimental technique: precision takes DPPH 3mg, is placed in the brown volumetric flask of 100ml, uses the dehydrated alcohol standardize solution, stored refrigerated.Sample, DPPH solution according to adding shown in table 7, after placing 30min, are measured light absorption value A, the clearance rate of calculation sample solution to DPPH under 517nm.Sample is as follows to the clearance rate computing formula of DPPH: the clearance rate of DPPH=[1-(A
I-A
J)/A
C] * 100%.
Table 7 application of sample program
4.2. experimental result: licoflavone and Vc ethylether are a kind of vegetable active additives commonly used in cosmetics, mainly play whitening, antioxidation, uvioresistant, antidotal effect.1% screen is driven quick formula extraction, 1%Vc ethylether and 1% licoflavone the clearance rate of DPPH is respectively 91.96%, 87.38% and 74.99%.Can see from experimental result, in the situation that add same amount, the imperial quick extract of screen is better than licoflavone and Vc ethylether to the removing effect of DPPH.
By above experiment confirm, " screen is driven quick side " extract and contain the cosmetics of described active component has clear and definite antiallergic, moisturizing and anti-oxidation efficacy, and safe, and is non-stimulated to human body.
Description of drawings
Fig. 1 screen is driven quick formula extraction extraction process
Fig. 2 is the skin irritation test report repeatedly
The imperial quick formula extraction of Fig. 3 screen discharges the inhibitory action of beta-amino hexose glycosidase to the RBL-2H3 cell
The imperial quick formula extraction of Fig. 4 screen discharges the inhibitory action of histamine to the RBL-2H3 cell
Fig. 5 sensation of pricking result of the test (accepting numbering CS-2010-25)
Fig. 6 sensation of pricking result of the test (accepting numbering CS-2010-25)
Fig. 7 sensation of pricking result of the test (accepting numbering CS-2010-26)
Fig. 8 sensation of pricking result of the test (accepting numbering CS-2010-26)
The instant hydration meansigma methods of Fig. 9 skin and skin hydration increment rate
Figure 10 TWEL meansigma methods and TWEL value rate of change
The specific embodiment
Take Rhizoma Atractylodis Macrocephalae 18g, give as one thinks fit cataclasm.Adopt the light oil extractor, add the 180g distilled water, extract volatile oil with steam distillation, extraction time is 5 hours, places a moment after stopped heating, collects volatile oil.
Take Radix Astragali 30g, Radix Saposhnikoviae 18g, Flos Chrysanthemi 9g, Herba Portulacae 18g, Radix Scutellariae 18g, Radix Sophorae Flavescentis 9g, Fructus Mume 9g, Radix Glycyrrhizae 18g, give as one thinks fit cataclasm.With 70% ethanol extraction twice, extracted 2 hours with the 1935g solvent for the first time, extracted 2 hours with the 1290g solvent for the second time.Merge extractive liquid, reclaims ethanol, and is concentrated, filters, and filtrate is with aqueous dispersion, and upper D101 type macroporous resin with the ethanol elution of water and variable concentrations gradient, is collected 30～70% ethanol elution respectively, reclaims solvent, evaporate to dryness.
Get above-mentioned volatile oil and powder, be dissolved in (propylene glycol: water=3: 5 (volume ratio)), obtain rufous and be clear to translucent liquid in the aqueous solution of propylene glycol according to the ratio of 1: 200 (weight ratio).
Take Rhizoma Atractylodis Macrocephalae 27g, give as one thinks fit cataclasm.Adopt the light oil extractor, add the 405g distilled water, extract volatile oil with steam distillation, extraction time is 5 hours, places a moment after stopped heating, collects volatile oil.
Take Radix Astragali 45g, Radix Saposhnikoviae 27g, Flos Chrysanthemi 13.5g, Herba Portulacae 27g, Radix Scutellariae 27g, Radix Sophorae Flavescentis 13.5g, Fructus Mume 13.5g, Radix Glycyrrhizae 27g, give as one thinks fit cataclasm.With 70% ethanol extraction twice, extracted 2 hours with the 2902g solvent for the first time, extracted 2 hours with the 1935g solvent for the second time.Merge extractive liquid, reclaims ethanol, and is concentrated, filters, and filtrate is with aqueous dispersion, and upper D101 type macroporous resin with the ethanol elution of water and variable concentrations gradient, is collected 30～70% ethanol elution respectively, reclaims solvent, evaporate to dryness.
Get above-mentioned volatile oil and powder, be dissolved in (propylene glycol: water=1: 5 (volume ratio)), obtain rufous and be clear to translucent liquid in the aqueous solution of propylene glycol according to the ratio of 1: 100 (weight ratio).
Take Rhizoma Atractylodis Macrocephalae 67.2g, give as one thinks fit cataclasm.Adopt the light oil extractor, add the 1008g distilled water, extract volatile oil with steam distillation, extraction time is 5 hours, places a moment after stopped heating, collects volatile oil.
Take Radix Astragali 112g, Radix Saposhnikoviae 67.2g, Flos Chrysanthemi 33.6g, Herba Portulacae 67.2g, Radix Scutellariae 67.2g, Radix Sophorae Flavescentis 33.6g, Fructus Mume 33.6g, Radix Glycyrrhizae 33.6g, give as one thinks fit cataclasm.With 70% ethanol extraction twice, extracted 2 hours with the 7224g solvent for the first time, extracted 2 hours with the 4816g solvent for the second time.Merge extractive liquid, reclaims ethanol, and is concentrated, filters, and filtrate is with aqueous dispersion, and upper D101 type macroporous resin with the ethanol elution of water and variable concentrations gradient, is collected 30～70% ethanol elution respectively, reclaims solvent, evaporate to dryness.
Get above-mentioned volatile oil and powder, be dissolved in (propylene glycol: water=5: 5 (volume ratio)), obtain rufous and be clear to translucent liquid in the aqueous solution of propylene glycol according to the ratio of 1: 50 (weight ratio).
Formulation Example 1
Peace skin facial milk cleanser weight (consumption %)
Chinese medicine compound " screen is driven quick side " extract 1～20
Cocos nucifera oil acyl Glycine sodium (25%) 10～40
Lauryl hydroxyl sulfo betaine (30%) 2～10
LAA (30%) 2～10
Acrylic acid (ester) analog copolymer (30%) 0.2～2
Essence is appropriate
Antiseptic is appropriate
Deionized water adds to 100
Formulation Example 2
Peace skin opsonic water weight (consumption %)
Chinese medicine compound " screen is driven quick side " extract 1～20
Essence is appropriate
Antiseptic is appropriate
Cremophor RH40 0.01～0.2
Deionized water adds to 100
Formulation Example 3
The peace skin nourishes emulsion weight (consumption %)
Chinese medicine compound " screen is driven quick side " extract 1～20
C
14-22Pure and mild C
12-20Alkyl androstanediol 1.5～2.5
Shea butter 0.5～1
VE 0.5～1
Xanthan gum 0.1～0.2
Essence is appropriate
Antiseptic is appropriate
Deionized water adds to 100
Formulation Example 4
Peace skin moisturizing curdled milk weight (consumption %)
Chinese medicine compound " screen is driven quick side " extract 1～20
V
E 0.1～1
Carbomer 0.1～0.2
Acrylic acid (ester) class/C
10-30Alkylol acrylamide acid esters cross linked polymer 0.1～0.3
Triethanolamine 0.2～0.5
Polysorbate-20 0.1～0.5
Essence is appropriate
Antiseptic is appropriate
Deionized water adds to 100
Formulation Example 5
The peace skin protective skin cream weight (consumption %) of releiving
Chinese medicine compound " screen is driven quick side " extract 1～20
The pure and mild cetearyl glucoside 2.5～4 of cetearyl alcohol
Caprylic/capric triglyceride 3～6
VE 0.2～1
Xanthan gum 0.1～0.3
Essence is appropriate
Antiseptic is appropriate
Deionized water adds to 100
Formulation Example 6
The peace skin releive the sleep facial film weight (consumption %)
Chinese medicine compound " screen is driven quick side " extract 1～20
Two propyl group alcohol 3～8
Encircle five polydimethylsiloxane 1～3
Polysorbate-20 0.1～0.5
Acrylic acid (ester) class/C
10-30Alkylol acrylamide acid esters cross linked polymer 0.2～0.4
Triethanolamine 0.2～0.4
Essence is appropriate
Antiseptic is appropriate
Deionized water adds to 100
Formulation Example 7
Peace skin mask weight (consumption %)
Chinese medicine compound " screen is driven quick side " extract 1～20
Trehalose 0.5～1
Hyaluronate sodium 0.01～0.1
Essence is appropriate
Solubilizing agent is appropriate
Antiseptic is appropriate
Deionized water adds to 100
Formulation Example 8
Peace skin aroma calm relaxing bath and shower cream weight (consumption %)
Chinese medicine compound " screen is driven quick side " extract 1～15
Sodium laureth sulfate (70%) 15～25
Cocamido propyl betaine (30%) 5～10
Cocos nucifera oil acyl Glycine sodium (25%) 10～20
Coconut oleoyl amine DEA 2～5
Sodium chloride 0.5～1.5
Citric acid is appropriate
Essence is appropriate
Antiseptic is appropriate
Deionized water adds to 100
Industrial applicibility
Chinese medicine compound of the present invention " screen is driven quick side " extract compatibility is reasonable, preparation technology's simple possible.Extract belongs to natural origin, and safe to skin has no untoward reaction.Effect with clear and definite anti-skin allergy, moisturizing and opposing skin aging.The cosmetics that contain " screen is driven quick side " of the present invention Chinese medicine extract have also had both the characteristic of the present invention's " screen is driven quick side " Chinese medicine extract.
Claims (7)
- A Chinese medicine composite extract by comprise the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae, Flos Chrysanthemi, Herba Portulacae, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Mume, Radix Glycyrrhizae totally nine flavor Chinese herbal medicine be prepared from, each composition percentage by weight is 19.39～21.42%, 11.63～12.86%, 11.63～12.86%, 5.82～6.43%, 11.63～12.86%, 11.63～12.86%, 5.82～6.43%, 5.82～6.43%, 11.63～12.86%, it is characterized in that:(1) take the Rhizoma Atractylodis Macrocephalae, give as one thinks fit cataclasmly, add 10～20 times of weight pure water, extract volatile oil with steam distillation, extraction time is 5 hours, places a moment after stopped heating, collects volatile oil 1(2) take the Radix Astragali, Radix Saposhnikoviae, Flos Chrysanthemi, Herba Portulacae, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Mume, Radix Glycyrrhizae, give as one thinks fit cataclasmly, with twice of 50～70% ethanol extraction: the solvent with 15 times of weight extracted 2 hours for the first time, solvent with 10 times of weight extracted 2 hours for the second time, and merge extractive liquid, reclaims ethanol, concentrated, filter, with aqueous dispersion, upper D101 type macroporous resin, respectively with the ethanol elution of water and variable concentrations gradient, collect 30～70% ethanol elution, reclaim solvent, obtain effective ingredient 2(3) get appropriate volatile oil 1 and effective ingredient 2, be dissolved in (propylene glycol: water=1: 5～5: 5 (volume ratio)) in the aqueous solution of propylene glycol according to the ratio of 1: 50～1: 200 (weight ratio), obtain rufous and be clear to translucent liquid, its PH scope is 4.5～6.5.
- 2. the purposes of Chinese herbal compounds as claimed in claim 1 in preparation antiallergic, moisturizing, antioxidative compositions.
- 3. a cosmetic composition, is characterized in that containing Chinese herbal compounds as claimed in claim 1.
- 4. cosmetic composition as claimed in claim 1, its Chinese medicine extraction complex is 1～20%, based on the gross weight of cosmetic complex.
- 5. cosmetic composition as claimed in claim 1, it is facial milk cleanser, surfactant, skin-care milk curd, protective skin cream, facial treatment essence liquid, facial film, mask, bath gel.
- 6. cosmetic composition as claimed in claim 1, it is emulsion, water preparation, cream frost, gel, pad pasting, lotion.
- 7. cosmetic composition as claimed in claim 1, it also can contain other beneficiating ingredient: water, emollient, wetting agent, surfactant, emulsifying agent, antioxidant, aromatic, thickening agent, nertralizer, screening agent, vitamin and antiseptic, and their mixture.
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| CN201110395990XA CN103127269A (en) | 2011-12-05 | 2011-12-05 | Extractive of Chinese herbal compound and cosmetics including active ingredients |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110395990XA CN103127269A (en) | 2011-12-05 | 2011-12-05 | Extractive of Chinese herbal compound and cosmetics including active ingredients |
Publications (1)
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Family
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| CN201110395990XA Pending CN103127269A (en) | 2011-12-05 | 2011-12-05 | Extractive of Chinese herbal compound and cosmetics including active ingredients |
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| CN103637973A (en) * | 2013-12-02 | 2014-03-19 | 太仓市伟基生物科技有限公司 | Skin-protecting and itch-relieving bath cream |
| CN104055702A (en) * | 2014-06-11 | 2014-09-24 | 滨州安华生物工程有限公司 | Anti-allergy skin moisturizer |
| CN104224629A (en) * | 2014-09-23 | 2014-12-24 | 珠海市时代经典化妆品有限公司 | Cosmetic with functions of tightening skin and relaxing muscles and stimulating blood circulation for body |
| CN104352598A (en) * | 2014-11-26 | 2015-02-18 | 河北科技师范学院 | Chinese herbal medicine composition for prevention and control of chicken diarrhea |
| CN104434726A (en) * | 2014-11-15 | 2015-03-25 | 柳州市康小乐牛奶有限公司 | Foot cream with capability of removing tinea pedis |
| CN104983633A (en) * | 2015-07-10 | 2015-10-21 | 北京国济众芳中医药研究所 | External traditional-Chinese-medicine composition with effects of relieving itching, firming hairs and removing free radicals, preparation and preparation method thereof |
| CN105796425A (en) * | 2016-04-07 | 2016-07-27 | 苏州蔻美新材料有限公司 | Moisturizing agent and preparation method thereof |
| CN106309911A (en) * | 2016-08-19 | 2017-01-11 | 上海家化联合股份有限公司 | Anti-ageing traditional Chinese medicine composition and preparation method thereof |
| CN109549883A (en) * | 2017-09-26 | 2019-04-02 | 东莞自然衡健康科技有限公司 | A kind of plant antiallergic is releived the preparation method of agent |
| CN109718185A (en) * | 2019-03-12 | 2019-05-07 | 北温草生物科技(上海)有限公司 | One kind is releived antianaphylactic herbal composite |
| CN109758391A (en) * | 2018-12-06 | 2019-05-17 | 浙江大学自贡创新中心 | It is a kind of to have effects that the plant composition of antiallergic |
| WO2019233158A1 (en) * | 2018-06-08 | 2019-12-12 | 苏州思源天然产物研发有限公司 | Traditional chinese medicine gel composition, preparation method and use thereof |
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| CN103637973A (en) * | 2013-12-02 | 2014-03-19 | 太仓市伟基生物科技有限公司 | Skin-protecting and itch-relieving bath cream |
| CN104055702A (en) * | 2014-06-11 | 2014-09-24 | 滨州安华生物工程有限公司 | Anti-allergy skin moisturizer |
| CN104055702B (en) * | 2014-06-11 | 2016-12-07 | 山东安华生物医药股份有限公司 | A kind of skin antiallergic wetting agent |
| CN104224629A (en) * | 2014-09-23 | 2014-12-24 | 珠海市时代经典化妆品有限公司 | Cosmetic with functions of tightening skin and relaxing muscles and stimulating blood circulation for body |
| CN104434726A (en) * | 2014-11-15 | 2015-03-25 | 柳州市康小乐牛奶有限公司 | Foot cream with capability of removing tinea pedis |
| CN104352598A (en) * | 2014-11-26 | 2015-02-18 | 河北科技师范学院 | Chinese herbal medicine composition for prevention and control of chicken diarrhea |
| CN104983633B (en) * | 2015-07-10 | 2018-02-09 | 北京国济众芳中医药研究所 | External medicine composition, preparation of free radical effect and preparation method thereof is removed with antipruritic strengthen the hair |
| CN104983633A (en) * | 2015-07-10 | 2015-10-21 | 北京国济众芳中医药研究所 | External traditional-Chinese-medicine composition with effects of relieving itching, firming hairs and removing free radicals, preparation and preparation method thereof |
| CN105796425A (en) * | 2016-04-07 | 2016-07-27 | 苏州蔻美新材料有限公司 | Moisturizing agent and preparation method thereof |
| CN106309911A (en) * | 2016-08-19 | 2017-01-11 | 上海家化联合股份有限公司 | Anti-ageing traditional Chinese medicine composition and preparation method thereof |
| CN106309911B (en) * | 2016-08-19 | 2021-01-22 | 上海家化联合股份有限公司 | Anti-aging traditional Chinese medicine composition and preparation method thereof |
| CN109549883A (en) * | 2017-09-26 | 2019-04-02 | 东莞自然衡健康科技有限公司 | A kind of plant antiallergic is releived the preparation method of agent |
| WO2019233158A1 (en) * | 2018-06-08 | 2019-12-12 | 苏州思源天然产物研发有限公司 | Traditional chinese medicine gel composition, preparation method and use thereof |
| CN109758391A (en) * | 2018-12-06 | 2019-05-17 | 浙江大学自贡创新中心 | It is a kind of to have effects that the plant composition of antiallergic |
| CN109718185A (en) * | 2019-03-12 | 2019-05-07 | 北温草生物科技(上海)有限公司 | One kind is releived antianaphylactic herbal composite |
| CN113693987A (en) * | 2021-09-16 | 2021-11-26 | 陈克 | Traditional Chinese medicine composition with moisturizing effect and extract and application thereof |
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Application publication date: 20130605 |