CN105603029A - Extraction method of active walnut peptides - Google Patents
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Abstract
The invention discloses an extraction method of active walnut peptides. The method comprises the following steps: screening dry walnut oil pulp subjected to cold oil pressing to obtain slag a, and carrying out ultrasonic extraction on the slag a twice to obtain a supernatant c; carrying out membrane concentration on the supernatant c to obtain a concentrated solution a; carrying out enzymolysis on the concentrated solution a twice to obtain an enzymolysis solution; carrying out membrane separation on the enzymolysis solution to obtain a low-molecular-weight polypeptide solution; carrying out desalting concentration on the low-molecular-weight polypeptide solution through a nanofiltration membrane to obtain a concentrated solution b; carrying out decolorization on the concentrated solution b to obtain a decolorized solution e; and carrying out desalting concentration on the solution e through a nanofiltration membrane to obtain a concentrated solution c, and carrying out vacuum concentration and spray drying on the concentrated solution c to obtain the walnut peptide product. The production technique for extracting walnut peptides from walnut oil pulp has the advantages of high extraction rate, high product activity, pure product color, low cost, no environment pollution and the like, is simple and safe to operate in the production process, and is convenient for industrialized large-scale production.
Description
Technical field
The invention belongs to technical field of bioengineering, relate to the extracting method of active walnut peptide.
Background technology
Walnut peptide can Promote immunity tissue functional rehabilitation, improve immunity of organisms, have and promote nucleosides to absorb and the effect of running, adjustable gut flora, is effectively to work in coordination with the prebiotics that probio plays a role, and well sobers up in addition, liver-protecting function. Walnut peptide has good dissolubility and has the low viscous functional characteristic of high concentration, is to produce the good raw material of high protein fluid food. The physiologically active that walnut peptide has is as absorption easy to digest, promotion microorganism are fermented, antioxidation activity can be used as raw material, the additive of producing fermented food, or the base-material of functional food.
At present, walnut peptide production technology directly adopts walnut oil meal hydro-thermal refluxing extraction, enzymolysis, activated carbon decolorizing, sephadex separation, evaporation and concentration and the such technical process of vacuum drying mostly, it is low that it produces recovery rate, extract, the high temperature heavy damage of concentration process activity and the color and luster of walnut peptide, and the residue severe contamination of leaching process environment, cause China's walnut peptide to can not get promotion and application effectively.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the invention provides a kind of extracting method of active walnut peptide.
The present invention adopts following technical scheme, and the extracting method of active walnut peptide is implemented according to following steps:
Step 1, obtain slag charge a after the dry walnut oil meal after pressed oil is screened, then slag charge a is carried out to twice ultrasonic extraction, obtain supernatant c;
Step 2, that supernatant c is carried out to film is concentrated, and obtains concentrate a;
Step 3, concentrate a is carried out to enzymolysis twice, obtain enzymolysis liquid;
Step 4, enzymolysis liquid is carried out to film separation, obtain little molecular weight polypeptide solution;
Step 5, to little molecular weight polypeptide solution NF membrane desalination and concentration, obtain concentrate b;
Step 6, again concentrate b is decoloured and obtains decolouring solution after treatment, be designated as solution e;
Step 7, again solution e is obtained to concentrate c by NF membrane desalination and concentration, by concentrate c Vacuum Concentration and spraying after dry walnut peptide product.
Further, in step 1, to use sieve diameter be 0.425mm the screen cloth dry walnut oil meal crushed material after to pressed oil screens, and obtains slag charge a.
Further, in step 1, the Na that is 0.1% by mass percentage concentration2S03Aqueous solution soaking slag charge a20～40min, and obtain mixed solution, be designated as solution a, wherein, Na2S03The weight ratio of the aqueous solution and slag charge a is 6:1; After solution a adds clear water, obtain mixed solution again, be designated as solution b, wherein, Na2S03The weight ratio of the gross weight of the aqueous solution and clear water and slag charge a is 20:1;
With dilute alkaline soln, the pH value of solution b is transferred to 7.5～8.5 again, and at the temperature of 50 DEG C～60 DEG C, carries out ultrasonic agitation and extract 1h, by centrifugal supernatant a and the slag charge b of obtaining of tube centrifuge; Adding pH value to slag charge b is again that the water of 8.0-9.0 obtains solution c, the weight ratio of the water adding and slag charge b is 20:1, at the temperature of 50 DEG C～60 DEG C, solution c is carried out to ultrasonic agitation and extract 1h, by the centrifugal supernatant b that obtains of tube centrifuge, finally supernatant a and supernatant b are mixed to get to supernatant c.
Further, in step 2, the concentrated concrete grammar of film is: by supernatant c ceramic membrane filter, obtain filtrate a, then by NF membrane, filtrate a to be concentrated into concentration of substrate be 3.5～4.5%, and obtain concentrate a.
Further, in step 3, the concrete grammar of enzymolysis is: be that 3 ‰ alkali protease obtains solution d to adding mass percentage concentration in concentrate a, and at the temperature of 50-55 DEG C, solution d is stirred to enzymolysis 2 hours, make the pH value of solution d remain 8.5～9.5, after the pH value of solution d drops between 7-8, adding mass percentage concentration is 3 ‰ neutral proteinase again, and at the temperature of 50-55 DEG C, stirs enzymolysis 2 hours, obtains enzymolysis liquid.
Further, in step 4, enzymolysis liquid is adjusted to faintly acid with diluted acid, then to enzymolysis liquid precipitate and centrifugal after obtain supernatant d; Supernatant d is adjusted into after neutrality with sig water, then uses milipore filter ultrafiltration, obtain little molecular weight polypeptide solution.
Further, in step 4, it is 4-5 that described enzymolysis liquid is adjusted to pH value with diluted acid, the more centrifugal supernatant d that obtains after precipitating 25～35 minutes.
Further, the concrete grammar that step 6 is decoloured is: the alcohol immersion that is 95% by concentration expressed in percentage by volume by non-polar macroporous resin 24 hours, wash with water again to without alcohol taste, soak 2 hours with the NaOH solution of molar concentration 1.0mol/L again, wash with water to neutrality again, then with molar concentration 1.0mol/LHCl solution immersion 2 hours, finally wash non-polar macroporous resin to neutral, use through above-mentioned macroreticular resin after treatment again concentrate b is decoloured, solution after treatment obtains decolouring.
Further, in step 7, it is 1.1～1.3Kg/cm that concentrate c is concentrated in vacuo to density3, and to the concentrate c walnut peptide product of spraying after dry to obtain.
The invention has the beneficial effects as follows, it is high that the production technology of extracting walnut peptide from walnut oil meal has recovery rate, and product is energetic, color and luster is pure, and production process is simple to operate, safety, cost is low, environmentally safe and be convenient to the outstanding advantages such as large-scale industrialization production.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in detail.
The invention provides a kind of extracting method of active walnut peptide, specifically implement according to following steps:
Step 1, to use sieve diameter be 0.425mm the screen cloth dry walnut oil meal crushed material after to pressed oil screens, and obtains slag charge a; The Na that is 0.1% by mass percentage concentration2S03Aqueous solution soaking slag charge a20～40min, and obtain mixed solution, be designated as solution a, wherein, Na2S03The weight ratio of the aqueous solution and slag charge a is 6:1; After solution a adds clear water, obtain mixed solution again, be designated as solution b, wherein, Na2S03The weight ratio of the gross weight of the aqueous solution and clear water and slag charge a is 20:1; With molar concentration 0.5mol/LNaOH solution, the pH value of solution b is transferred to 7.5～8.5 again, and at the temperature of 50 DEG C～60 DEG C, solution b is carried out to ultrasonic agitation and extract 1h, by centrifugal supernatant a and the slag charge b of obtaining of tube centrifuge; Adding pH value to slag charge b is again that the water of 8.0-9.0 obtains solution c, the weight ratio of the water adding and slag charge b is 20:1, at the temperature of 50 DEG C～60 DEG C, solution c is carried out to ultrasonic agitation and extract 1h, by the centrifugal supernatant b that obtains of tube centrifuge, finally supernatant a and supernatant b are mixed to get to supernatant c.
Step 2, by supernatant c ceramic membrane filter, obtain filtrate a, then by NF membrane, filtrate a to be concentrated into concentration of substrate be 3.5～4.5%, and obtain concentrate a.
Step 3, to add mass percentage concentration to concentrate a be 3 ‰ alkali protease m gram, under the temperature conditions of 55 DEG C, stir enzymolysis and after 2 hours, obtain solution d, in 2 hours, regulating the pH value of maintenance solution d with molar concentration 0.5mol/LNaOH solution is 8.5～9.5, after 2 hours, no longer add molar concentration 0.5mol/LNaOH solution, after the pH value of solution d drops between 7-8, adding mass percentage concentration is 3 ‰ neutral proteinase m gram again, at 50-55 DEG C, stir enzymolysis 2 hours, obtain enzymolysis liquid. Wherein, add the ratio of 2.5～3.5mg alkali protease according to the concentrate a of every 1000ml, add alkali protease according to the actual volume of concentrate a, and the addition of neutral proteinase and the addition of alkali protease equate.
Step 4, enzymolysis liquid is adjusted to faintly acid with molar concentration 0.5mol/LHCl solution, then to enzymolysis liquid precipitate and centrifugal after obtain supernatant d; Supernatant d is adjusted into after neutrality with molar concentration 0.5mol/LNaOH solution, then it is carried out to ultrafiltration with milipore filter, obtain little molecular weight polypeptide solution. Wherein, faintly acid is pH value 4-5, and the sedimentation time is 25～35 minutes.
Step 5, to little molecular weight polypeptide solution NF membrane desalination and concentration, obtain concentrate b.
Step 6, the alcohol immersion that is 95% by non-polar macroporous resin LX-T25H by volumetric concentration 24 hours, wash with water again to without alcohol taste, soak 2 hours with molar concentration 1.0mol/LNaOH solution again, wash with water again to neutrality, soak 2 hours with molar concentration 1.0mol/LHCl solution again, finally wash non-polar macroporous resin to neutral, then use through above-mentioned macroreticular resin after treatment concentrate b is decoloured, the solution after treatment that obtains decolouring, is designated as solution e. Wherein, the above-mentioned consumption for the ethanol, NaOH solution and the HCl solution that soak is all according to the requirement of this area production technology, and selects according to the consumption of non-polar macroporous resin.
Step 7, again solution e is obtained to concentrate c by NF membrane desalination and concentration, it is 1.1～1.3Kg/cm that concentrate c is concentrated in vacuo to density3, and to the concentrate c walnut peptide product of spraying after dry to obtain.
Embodiment 1:
To use sieve diameter be 0.425mm the screen cloth dry walnut oil meal crushed material after to pressed oil screens, and gets 300 grams of walnut oil meals through screening, the Na that is 0.1% by 1800m mass percentage concentration2S03Aqueous solution soaking walnut oil meal 30min, add wherein again the water of 4200ml, and its pH value is adjusted into 8.0 with the NaOH solution of molar concentration 0.5mol/L, under the temperature conditions of 55 DEG C, use ultrasonic agitation to extract 1h, finally by tube centrifuge centrifugal supernatant a and slag charge b;
Adding pH value to slag charge b is the water of 8.0-9.0, and the weight ratio of the water adding and slag charge b is 20:1, uses ultrasonic agitation to extract 1h under the temperature conditions of 55 DEG C, then by the centrifugal supernatant b that obtains of tube centrifuge.
Supernatant a and supernatant b merging ceramic membrane filter, the filtrate after filtration is 4% by the mass concentration that NF membrane is concentrated into substrate, obtains concentrate a2000ml.
Adding mass percentage concentration to concentrate a is 3 ‰ alkali protease 0.21g, under the temperature conditions of 55 DEG C, stir enzymolysis and after 2 hours, obtain solution d, in 2 hours, regulating the pH value of maintenance solution d with molar concentration 0.5mol/LNaOH solution is 9.0, after 2 hours, no longer add molar concentration 0.5mol/LNaOH solution, after the pH value of solution d drops between 7-8, adding mass percentage concentration is 3 ‰ neutral proteinase 0.21g, stirs enzymolysis 2 hours at 50-55 DEG C, obtains enzymolysis liquid.
It is between 4-5 that enzymolysis liquid is adjusted to pH value with molar concentration 0.5mol/LHCl solution, again enzymolysis liquid is precipitated to 30 minutes, centrifuging and taking supernatant d, and the pH value of supernatant d is transferred to neutrality, with milipore filter, supernatant d is filtered again, obtain the polypeptide solution of little molecular weight.
By the polypeptide solution of little molecular weight NF membrane desalination and concentration, obtain concentrate b1500ml, the non-polar macroporous resin post decolouring that the upper pretreatment of concentrate b is good, destainer NF membrane desalination and concentration, be concentrated into small size with rotary evaporator again, vacuum drying, obtains walnut peptide product 27.8g.
Wherein, to the pretreated method of non-polar macroporous resin post be: the alcohol immersion that is 95% by non-polar macroporous resin LX-T25H concentration expressed in percentage by volume 24 hours, wash with water again to without alcohol taste, soak 2 hours with molar concentration 1.0mol/LNaOH solution again, wash with water again to neutrality, soak 2 hours with molar concentration 1.0mol/LHCl solution again, finally wash non-polar macroporous resin to neutral.
Embodiment 2:
To use sieve diameter be 0.425mm the screen cloth dry walnut oil meal crushed material after to pressed oil screens, and gets the walnut oil meal that double centner crushes through screening, and adding 600L mass percentage concentration to it is 0.1%Na2S03The aqueous solution also soaks 30min, then adds wherein 1400L water, and its pH value is adjusted into PH9.0 with dilute alkaline soln, uses ultrasonic agitation to extract 1h under the temperature conditions of 50 DEG C-60 DEG C, finally by tube centrifuge centrifugal supernatant a and slag charge b;
Adding pH value to slag charge b is the water of 8.0-9.0, and the weight ratio of the water adding and slag charge b is 20:1, uses ultrasonic agitation to extract 1h under the temperature conditions of 50 DEG C-60 DEG C, by the centrifugal supernatant b that obtains of tube centrifuge.
Supernatant a and supernatant b merging ceramic membrane filter, the filtrate that filtration obtains is concentrated into the mass concentration 4% of substrate by NF membrane, obtain concentrate a640L.
Adding mass fraction to concentrate a is that to add mass percentage concentration be 3 ‰ alkali protease 75g, under the temperature conditions of 55 DEG C, stir enzymolysis and after 2 hours, obtain solution d, in 2 hours, regulating the pH value of maintenance solution d with the NaOH solution of molar concentration 0.5mol/L is 9.0, after 2 hours, no longer add molar concentration 0.5mol/LNaOH solution, after the pH value of solution d drops between 7-8, adding 75g mass percentage concentration is 3 ‰ neutral proteinase, at 50-55 DEG C, stir enzymolysis 2 hours, obtain enzymolysis liquid.
It is between 4-5 that enzymolysis liquid is adjusted to PH with molar concentration 0.5mol/LHCl solution, again enzymolysis liquid is precipitated to 30 minutes, centrifuging and taking supernatant is adjusted d, and the pH value of supernatant d is transferred to neutrality, with milipore filter, supernatant d is filtered again, obtain the polypeptide solution of little molecular weight.
By the polypeptide solution of little molecular weight NF membrane desalination and concentration, obtain concentrate b520L, the resin column decolouring that the upper pretreatment of concentrate b is good, destainer NF membrane desalination and concentration, be concentrated into small size with vacuum concentrator again, spraying is dry, obtains 9.8 kilograms of walnut peptide products.
Wherein, to the pretreated method of non-polar macroporous resin post be: the alcohol immersion that is 95% by non-polar macroporous resin LX-T25H concentration expressed in percentage by volume 24 hours, wash with water again to without alcohol taste, soak 2 hours with molar concentration 1.0mol/LNaOH solution again, wash with water again to neutrality, soak 2 hours with molar concentration 1.0mol/LHCl solution again, finally wash non-polar macroporous resin to neutral.
The denaturation temperature of walnut protein is 67.05 DEG C, and walnut protein confrontation heat is comparatively responsive. Walnut peptide production technology directly adopts walnut oil meal hydro-thermal refluxing extraction, evaporation and concentration mostly at present, and more than 90 DEG C, high temperature extracts like this, and 70 DEG C above concentrated, causes the intensification of walnut protein inactivation and product color. And the present invention adopts ultrasonic agitation to extract and film concentration technique, solve the low problem of walnut peptide recovery rate under 55 DEG C of left and right temperature conditions, use lower extraction temperature, the problem of having deepened with regard to having avoided extracting walnut protein inactivation that concentrated high temperature causes and product color.
Walnut peptide health care the most effectively part is that molecular weight is 2000-3000 dalton. Walnut peptide production technology adopts sephadex to separate walnut peptide sized molecules mostly at present, causes separating technology complexity, and cost is high, suitability for industrialized production difficulty. The present invention adopts milipore filter separation equipment to remove large molecule and the little molecule in solution, and technique is simple, and cost is low, is easy to suitability for industrialized production.
Walnut peptide production technology adopts alkali protease, neutral proteinase hydrolysis walnut protein mostly at present, the disposable utilization of protease, and utilization rate is low, causes production cost higher. The present invention adopts immobilized enzyme hydrolysis technology, and protease repeatedly utilizes, and utilization rate is high, greatly reduces production costs. Immobilized enzyme hydrolysis technology is by being embedded in enzyme in gel, microcapsule, or be connected on solid phase carrier by covalent bond, ionic bond or absorption, or by crosslinking agent make enzyme molecule mutually the method such as crosslinked make enzyme insoluble or be confined to a process in limited space, enzyme Reusability be can make, enzyme and product separation are convenient to.
Wherein, the preparation process of immobilised enzymes is as follows:
1. take Powdered chitin 5g, add 1000ml5% glutaraldehyde solution, regulate pH=8.5 with 0.5mol/LNaOH solution, after stirring 25 DEG C of constant temperature joltings 1 hour. The taking-up glutaraldehyde that inclines, with distilled water washing 2 times, the stillness of night of inclining is to remove unnecessary crosslinking agent.
2. get enzyme liquid prepared by 1000ml, the chitin of processing is added and mixed, 25 DEG C of constant temperature joltings 1 hour, 4 DEG C of refrigerators were placed and are spent the night.
3. next day, use 4000rpm centrifugation, the clear liquid that inclines, distilled water washing obtains immobilised enzymes 2 times.
Walnut oil meal is pulverized too thick, is unfavorable for the stripping of protein, pulverizes too carefully, though be conducive to the stripping of protein, easily causes filtration difficulty, the prolongation production time. Walnut peptide production technology adopts plain gauze natural filtration mostly at present, and oil meal is pulverized and too carefully caused filtration difficulty, extends the production time. The present invention adopts tube centrifuge to carry out Separation of Solid and Liquid, has accelerated Separation of Solid and Liquid speed, has greatly reduced the production time.
The present invention also adopts non-polar macroporous resin to decolour to the solution colour in active walnut peptide production process, this non-polar macroporous resin is selected the model LX-T25H of Xi'an Sunresin New Materials Co., Ltd., can produce the active walnut peptide product of white or near-white, make product appearance good, be suitable in skin care item, food or skin care products and apply.
For addressing the above problem, we have carried out abundant research to the production technology of extracting walnut peptide from walnut oil meal, advanced production technology and the equipment such as ultrasound assisted extraction technique, film concentration technique, immobilized enzyme hydrolysis technology, film separation and desalting technology and continuous low temperature drying technology are adopted, it is high that the production technology that makes to extract walnut peptide from walnut oil meal has recovery rate, product is energetic, color and luster is pure, and production process is simple to operate, safety, cost is low, environmentally safe and be convenient to the outstanding advantages such as large-scale industrialization production.
Table 1
Table 2
Table 3
As seen from Table 1, adopt ultrasonic extracting method of the present invention to improve 34.38% than the recovery rate that adopts hot reflux extracting method; As seen from Table 2, adopt film method for concentration of the present invention to reduce by 27.29% than the loss late that adopts evaporation and concentration; As seen from Table 3, it is more little than adopting the recovery rate difference of gel separation method to adopt membrane separating method of the present invention, but 1/8 of the disengaging time that the disengaging time that adopts membrane separating method only separates for gel, the unit cost that adopts membrane separating method is only 1/5 of gel separation. To sum up, the extracting method of active walnut peptide of the present invention, recovery rate is high, loss late is low, cost is low.
The present invention is according to the chemical property of walnut peptide and equipment characteristic, the advanced production technologies such as ultrasound assisted extraction technique, film concentration technique, immobilized enzyme hydrolysis technology, film separation and desalting technology and continuous low temperature drying technology are organically combined, it is high that the production technology that makes to extract walnut peptide from walnut oil meal has recovery rate, product is energetic, color and luster is pure, and production process is simple to operate, safety, cost is low, environmentally safe, be convenient to large-scale industrialization produce.
Claims (9)
1. the extracting method of active walnut peptide, is characterized in that, implements according to following steps:
Step 1, obtain slag charge a after the dry walnut oil meal after pressed oil is screened, then slag charge a is carried outTwice ultrasonic extraction, obtains supernatant c;
Step 2, that supernatant c is carried out to film is concentrated, and obtains concentrate a;
Step 3, concentrate a is carried out to enzymolysis twice, obtain enzymolysis liquid;
Step 4, enzymolysis liquid is carried out to film separation, obtain little molecular weight polypeptide solution;
Step 5, to little molecular weight polypeptide solution NF membrane desalination and concentration, obtain concentrate b;
Step 6, again concentrate b is decoloured and obtains decolouring solution after treatment, be designated as solution e;
Step 7, again solution e is obtained to concentrate c by NF membrane desalination and concentration, by concentrate c Vacuum ConcentrationAnd spraying after dry walnut peptide product.
2. the extracting method of active walnut peptide as claimed in claim 1, is characterized in that, described step 1In, to use sieve diameter be 0.425mm the screen cloth dry walnut oil meal crushed material after to pressed oil screens,And obtain slag charge a.
3. the extracting method of active walnut peptide as claimed in claim 1 or 2, is characterized in that, described stepIn 1, the Na that is 0.1% by mass percentage concentration2S03Aqueous solution soaking slag charge a20～40min, and mixedSolution, is designated as solution a, wherein, and Na2S03The weight ratio of the aqueous solution and slag charge a is 6:1; Add to solution a againAfter entering clear water, obtain mixed solution, be designated as solution b, wherein, Na2S03The gross weight of the aqueous solution and clear water and slag chargeThe weight ratio of a is 20:1;
With dilute alkaline soln, the pH value of solution b is transferred to 7.5～8.5 again, and enters at the temperature of 50 DEG C～60 DEG CRow ultrasonic agitation is extracted 1h, by centrifugal supernatant a and the slag charge b of obtaining of tube centrifuge; Add to slag charge b againPH value is that the water of 8.0-9.0 obtains solution c, and the weight ratio of the water adding and slag charge b is 20:1, at 50 DEG C～60 DEG CTemperature under solution c carried out to ultrasonic agitation extract 1h, by tube centrifuge centrifugal supernatant b, lastSupernatant a and supernatant b are mixed to get to supernatant c.
4. the extracting method of active walnut peptide as claimed in claim 1, is characterized in that, described step 2The concentrated concrete grammar of middle film is: by supernatant c ceramic membrane filter, obtain filtrate a, then with NF membrane willIt is 3.5～4.5% that filtrate a is concentrated into concentration of substrate, and obtains concentrate a.
5. the extracting method of active walnut peptide as claimed in claim 1, is characterized in that, described step 3The concrete grammar of middle enzymolysis is: be that 3 ‰ alkali protease obtains to adding mass percentage concentration in concentrate aSolution d, and at the temperature of 50-55 DEG C, solution d is stirred to enzymolysis 2 hours, make the pH value of solution dRemain 8.5～9.5, after the pH value of solution d drops between 7-8, then to add mass percentage concentration be 3 ‰Neutral proteinase, and at the temperature of 50-55 DEG C, stir enzymolysis 2 hours, obtain enzymolysis liquid.
6. the extracting method of active walnut peptide as claimed in claim 1, is characterized in that, described step 4In, enzymolysis liquid is adjusted to faintly acid with diluted acid, then to enzymolysis liquid precipitate and centrifugal after obtain supernatant d;Supernatant d is adjusted into after neutrality with sig water, then uses milipore filter ultrafiltration, obtain little molecular weight polypeptide solution.
7. the extracting method of active walnut peptide as claimed in claim 6, is characterized in that, described step 4In, it is 4-5 that described enzymolysis liquid is adjusted to pH value with diluted acid, the more centrifugal supernatant d that obtains after precipitating 25～35 minutes.
8. the extracting method of active walnut peptide as claimed in claim 1, is characterized in that, described step 6The concrete grammar of decolouring is: the alcohol immersion 24 that is 95% by non-polar macroporous resin concentration expressed in percentage by volume is littleTime, then wash with water to without alcohol taste, then soak 2 hours with the NaOH solution of molar concentration 1.0mol/L, thenWash with water to neutrality, then with molar concentration 1.0mol/LHCl solution immersion 2 hours, finally wash nonpolarMacroreticular resin is to neutral, then uses through above-mentioned macroreticular resin after treatment concentrate b is decoloured, and decolouredSolution after treatment.
9. the extracting method of the active walnut peptide as described in claim 1 or 8, is characterized in that, described stepIn rapid 7, it is 1.1～1.3Kg/cm that concentrate c is concentrated in vacuo to density3, and concentrate c is sprayedAfter dry, obtain walnut peptide product.
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| CN109527163A (en) * | 2018-11-21 | 2019-03-29 | 云南省林业科学院 | A kind of walnut polypeptide coffee and preparation method thereof |
| CN109527160A (en) * | 2018-11-21 | 2019-03-29 | 云南省林业科学院 | A kind of walnut polypeptide coffeemate and preparation method thereof |
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| CN107474941A (en) * | 2017-09-26 | 2017-12-15 | 绥化学院 | The method that aqueous enzymatic method synchronously extracts sesame oil and sesame polypeptide powder |
| CN107474941B (en) * | 2017-09-26 | 2020-11-06 | 绥化学院 | Method for synchronously extracting sesame oil and sesame polypeptide powder by aqueous enzymatic method |
| CN109527163A (en) * | 2018-11-21 | 2019-03-29 | 云南省林业科学院 | A kind of walnut polypeptide coffee and preparation method thereof |
| CN109527160A (en) * | 2018-11-21 | 2019-03-29 | 云南省林业科学院 | A kind of walnut polypeptide coffeemate and preparation method thereof |
| CN110089665A (en) * | 2019-05-14 | 2019-08-06 | 西安中粮工程研究设计院有限公司 | A kind of decolourize for walnut protein peptide bitter composition and method |
| CN114044811A (en) * | 2022-01-13 | 2022-02-15 | 中食都庆（山东）生物技术有限公司 | Walnut peptide and preparation method and application thereof |
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