CN114259430A

CN114259430A - Plant essential oil bacteriostatic spray and preparation method thereof

Info

Publication number: CN114259430A
Application number: CN202111618291.7A
Authority: CN
Inventors: 聂毅; 姚欢欢; 朱静静
Original assignee: Zhengzhou Institute of Emerging Industrial Technology
Current assignee: Zhengzhou Institute of Emerging Industrial Technology
Priority date: 2021-12-28
Filing date: 2021-12-28
Publication date: 2022-04-01
Anticipated expiration: 2041-12-28
Also published as: CN114259430B

Abstract

The invention discloses a plant essential oil bacteriostatic spray and a preparation method thereof, wherein the bacteriostatic spray comprises A, B, C three phases in 100 parts by weight, wherein A: 0.01-5 parts of spearmint essential oil, 0.01-1 part of lavender essential oil, 0.01-1 part of atractylis lancea essential oil, 0.01-1 part of wormwood essential oil, 0.01-1 part of sweet wormwood essential oil and 0.05-10 parts of propylene glycol; b: 0.01-1 part of phenoxyethanol and 0.1-0.5 part of octoxyglycerol; c: 0.01-0.1 part of sodium citrate, 0.01-0.05 part of bronopol, 200.5-10 parts of tween, 0.01-0.1 part of sodium hyaluronate, 0.01-0.3 part of defoaming agent and the balance of spearmint hydrosol. The antibacterial agent can effectively improve the antibacterial rate of pseudomonas aeruginosa, staphylococcus aureus and candida albicans, has an obvious skin moisturizing effect, is simple in process and easy to operate, and is safe and reliable, and the antibacterial agent has no toxic or side effect.

Description

Plant essential oil bacteriostatic spray and preparation method thereof

Technical Field

The invention belongs to the field of daily chemical articles, and particularly relates to a plant essential oil bacteriostatic spray and a preparation method thereof.

Background

Along with the enhancement of the health disinfection consciousness of residents, medical protection and disinfection articles can be continuously increased for a long time in the future, particularly, the required dosage of the disinfection articles can be increased in all regions of the whole country during a new crown epidemic situation, the market scale of the disinfection products can be continuously enlarged, the demand of disinfection and bacteriostasis spray in daily life is particularly indispensable, and the disinfection and bacteriostasis spray becomes a necessary article for the families and the trips of the masses. The existing bacteriostatic spray products on the market are various, however, various chemical synthesis substances are mostly added in the bacteriostatic spray products, and the substances are not easy to degrade and can cause environmental pollution; in addition, the bacteriostatic spray has single efficacy and only has the functions of disinfection and bacteriostasis. Therefore, the development of a bacteriostatic spray product containing natural plant active ingredients and having multiple functions is a great demand in the field of disinfection. The bacteriostatic spray can adjust the balance of flora, inhibit the growth of bacteria, prevent the bacteria from breeding too much and endanger health.

The research and utilization of essential oil at the present stage are relatively less, and Chinese patent CN 108938795A 'skin bacteriostatic spray and preparation process thereof' mainly introduces the mixing of concentrated solution of lavender essential oil, honeysuckle essential oil, large leaf fresh cinnamon and phellodendron bark and partial chemical cosolvent, wherein the preparation process of the concentrated solution of medicinal materials is relatively complicated; chinese patent CN 108164785A "a plant essential oil type master batch for killing and expelling insects, its preparation method and application" introduces mixing essential oil of herba Selaginellae Doederleinii, herba Artemisiae Anomalae, Murraya Koenigii, herba Menthae Rotundifoliae, etc. with antioxidant, pulvis Talci, etc., and is mainly applied to agricultural mulching film and greenhouse film for preventing insects and expelling insects; chinese patent CN 111700848A, "A plant bacteriostatic spray and its preparation method", introduces a plant bacteriostatic spray which is prepared by mixing and stirring combined essential oil of eucalyptus, Cinnamomum camphora, Verbenaceae, rosemary, etc., multiple plant compound extractive solutions, aromatic, cosolvent, etc., and has various kinds and complicated procedures.

The invention is researched and developed by multiple parties, and five kinds of natural plant essential oil of spearmint, lavender, rhizoma atractylodis, wormwood and sweet wormwood are selected, wherein the essential oil is composed of different organic compounds, mainly alcohols, aldehydes, acids, phenols, esters, ketones and terpenoids. The essential oil mainly comprises carvone as a main chemical component, and limonene, pinene, myrcene, limonene, eucalyptol, menthone and the like as other components, wherein the components have the effects of dispelling wind, relieving exterior syndrome, diminishing inflammation, relieving pain, resisting bacteria, killing insects and the like; the lavender essential oil has antioxidant, tranquilizing, smooth muscle relaxing, anticoagulant, mania resisting, anthelmintic, antifungal, hypnotic, and anxiolytic effects; the atractylis lancea essential oil mainly has the effects of spasmolysis, analgesia, antibiosis, antivirus and the like, and also has certain curative effects on the aspects of resisting anoxia, protecting the liver, treating gastric ulcer, regulating the gastrointestinal immune system and the like; the wormwood essential oil has the effects of regulating qi and blood, expelling cold and dampness, warming channels and the like; the essential oil mainly contains terpenoids, and has antibacterial, anticancer, antioxidant, antiinflammatory, heat clearing away, and mosquito repelling effects. The essential oil has wide application in the fields of daily necessities, food industry, medicine and the like.

The invention adopts the traditional process to extract essential oil and hydrolat in a plurality of plant raw materials such as spearmint, lavender, atractylis lancea, wormwood, sweet wormwood and the like, is green and pollution-free, has no solvent residue, and is also beneficial to conversion and mass production. Through the process technologies of compound addition, emulsification homogenization and the like, various natural active substance components are fused, the spearmint hydrosol is selected as a main solvent system, and chemical essence is not required to be added, so that the product is rich in fresh, cool and sweet smell, and the advantages are fully exerted. The product is mild, safe, non-irritant, harmless to human skin, mature in preparation process, low in equipment investment, stable in system integrity and capable of realizing batch production rapidly.

Disclosure of Invention

The invention aims to provide a plant essential oil bacteriostatic spray product and a preparation method thereof, and the prepared product has multiple effects of bacteriostasis, moisture preservation, inflammation diminishing, nerve soothing, skin care and the like, is applied to skin and mucosa sanitation, has no stimulation to skin and has good system stability.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a plant essential oil bacteriostatic spray product and a preparation method thereof comprise A, B, C three-phase components in parts by weight (100 parts), wherein the A phase comprises the following components: 0.01-5 parts of spearmint essential oil, 0.01-1 part of lavender essential oil, 0.01-1 part of atractylis lancea essential oil, 0.01-01 parts of wormwood essential oil, 0.01-1 part of sweet wormwood essential oil and 0.05-10 parts of propylene glycol; phase B: 0.01-1 part of phenoxyethanol and 0.1-0.5 part of octoxyglycerol; and C phase: 60-90 parts of spearmint hydrosol, 0.01-0.1 part of sodium citrate, 0.01-0.05 part of bronopol, 200.5-10 parts of tween, 0.01-0.1 part of sodium hyaluronate and 0.01-0.3 part of defoaming agent.

In the invention, the preparation processes of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, wormwood essential oil and sweet wormwood essential oil are respectively as follows:

weighing spearmint raw materials, placing the spearmint raw materials in a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking for 12-14 h, carrying out oil bath heating at 110 ℃ for 4h to obtain distilled volatile oil, taking out the volatile oil, continuing distilling for 2-3 h to obtain water content of the distilled water, adding a small amount of anhydrous sodium sulfate into the volatile oil for water absorption and drying, filtering to obtain the spearmint essential oil, and filtering the distilled water to obtain the spearmint hydrosol.

Weighing lavender raw materials, placing the lavender raw materials into a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking for 6-8 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain the lavender essential oil.

Weighing atractylis lancea raw material, placing into a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking for 10-12 h, heating in an oil bath at 110 ℃ for extraction for 6h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain the atractylis lancea essential oil.

Weighing wormwood raw materials, placing the wormwood raw materials into a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking for 12-16 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain the wormwood essential oil.

Weighing sweet wormwood raw materials, placing the sweet wormwood raw materials into a drying container, adding distilled water according to the liquid-material ratio of 10:1 (ml/g) by using a steam distillation method, soaking for 8-12 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain the sweet wormwood essential oil.

The preparation method of the plant essential oil bacteriostatic spray comprises the following steps:

(1) mixing propylene glycol with the above essential oil of herba Menthae Rotundifoliae, Lavender, Atractylodes lancea rhizome, folium Artemisiae Argyi and herba Artemisiae Annuae in weight parts, and dissolving to obtain A phase mixture;

(2) mixing phenoxyethanol and octoxyglycerol in parts by weight, and fully dissolving the phenoxyethanol and the octoxyglycerol to obtain a B-phase mixed solution;

(3) mixing the spearmint hydrosol obtained in the preparation process with sodium citrate, bronopol, tween-20, sodium hyaluronate and defoaming agent in parts by weight, and stirring at room temperature for 5-30min to obtain a C-phase mixed solution;

(4) and (3) mixing the B, C two-phase mixed solution obtained in the steps (2) and (3) with each other, stirring at room temperature for 30-60min, slowly adding the A-phase mixed solution obtained in the step (1) into the solution, stirring for 30-60min, and standing for 1-72h to obtain the bacteriostatic spray product.

The invention has the following advantages and beneficial effects:

(1) the essential oil and the hydrolat are extracted from the spearmint, the lavender, the atractylis lancea, the wormwood and the sweet wormwood, the component efficacy is clear, the safety and the toxic and side effects are avoided, the natural plants are fully utilized, the resource utilization rate is improved, and the antibacterial, nerve calming and eye-catching effects of the product are improved;

(2) the main solvent system of the invention is the spearmint hydrolat, and chemical essence is not needed to be added, so that the product is rich in fresh, cool and sweet smell, and is safe and non-irritant;

(3) the preparation method of the essential oil provided by the invention is a traditional water extraction process, is simple and easy to operate, well protects the active ingredients of the medicine in natural plants, has controllable cost, does not have organic solvent residue, and is green and pure natural;

(4) the preparation technology is mature, the process is simple, the operation time is greatly shortened, the efficiency is improved, full and uniform mixing can be realized without high-end production equipment such as a homogenizer, and the production cost is reduced;

(5) the invention realizes bacteriostasis and disinfection, the solvent volatilizes and is absorbed quickly, the residual substances can inhibit bacteria, disinfect, calm the nerves and preserve moisture, the skin is moist and not tight, and the invention is safe and has no stimulation;

(6) the invention meets the requirement of 'disinfection technical specification' of the Ministry of health, and aims at the skin and mucosa of human body, the main sterilization strains are staphylococcus aureus, pseudomonas aeruginosa and candida albicans;

(7) the invention meets the requirements in QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, and has obvious moisturizing effect on human skin;

(8) the invention is mainly obtained by adding various plant essential oil components and solvents into the spearmint hydrosol. The single plant essential oil has the effects of bacteriostasis, anti-inflammation, tranquilization, antipyresis, analgesia and the like, but has different main component contents, single bacteriostasis type and short time effect. According to the invention, the essential oils of a plurality of plants such as spearmint, lavender, atractylis lancea, wormwood and sweet wormwood contain organic compounds such as alcohols, aldehydes, acids, phenols, esters, ketones and terpenes, and under the combined action, the synergistic effect can be achieved, the bactericidal activity to staphylococcus aureus, pseudomonas aeruginosa, candida albicans and the like can be enhanced, the skin of a human body can be strengthened and calmed, and the effects of moisturizing, soothing and diminishing inflammation and the like can be achieved; the main solvent system is spearmint hydrosol which contains a small amount of chemical components such as carvone, limonene, pinene, myrcene, menthone and the like, has fresh smell and is refreshing; experimental results show that the optimal use effect can be obtained by using the plant essential oils and the hydrolat in a matching way.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.

The parts in the following examples and comparative examples represent grams.

Example 1 (product 8)

The preparation method of the spearmint essential oil, the spearmint hydrosol, the lavender essential oil, the atractylis lancea essential oil, the wormwood essential oil and the sweet wormwood essential oil in the embodiment is as follows:

weighing spearmint powder, placing the spearmint powder in a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a traditional steam distillation method, soaking for 12-14 h, carrying out oil bath heating at 110 ℃ for 4h to obtain distilled volatile oil, taking out the volatile oil, continuing distilling for 2-3 h to obtain water content of the distilled water, adding a small amount of anhydrous sodium sulfate into the volatile oil for water absorption and drying, filtering to obtain spearmint essential oil, and filtering the distilled water to obtain spearmint hydrosol; weighing lavender powder, placing into a drying container, adding distilled water according to a liquid-material ratio of 12 (ml/g) by using a traditional steam distillation method, soaking for 6-8 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain lavender essential oil; weighing Atractylodes lancea powder, placing into a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using the traditional steam distillation method, soaking for 10-12 h, heating in an oil bath at 110 ℃ for extraction for 6h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain Atractylodes lancea essential oil; weighing wormwood raw materials, placing the wormwood raw materials into a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a traditional steam distillation method, soaking for 12-16 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain wormwood essential oil; weighing sweet wormwood raw materials, placing the sweet wormwood raw materials into a drying container, adding distilled water according to the liquid-material ratio of 10:1 (ml/g) by using a traditional steam distillation method, soaking for 8-12 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain the sweet wormwood essential oil.

The preparation method of the plant essential oil bacteriostatic spray product of the embodiment is as follows (all raw materials are calculated by 100 parts by weight):

mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 0.5 part of lavender essential oil, 0.5 part of atractylis lancea essential oil, 0.5 part of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to fully dissolve the components to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.1 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the balance, namely the sum of all the components is 100 parts) in the preparation process with 0.1 part of sodium citrate, 0.01 part of bronopol, 205 parts of tween, 0.05 part of sodium hyaluronate and 0.05 part of defoaming agent, and stirring for 20min at room temperature to prepare a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 30min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 30min, and standing for 12h to obtain antibacterial spray product.

The experiment is carried out according to 2.1.8 antibacterial test in 'disinfection technical Specification', and the result shows that the antibacterial rate of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like is not 98.8%, 100% and 97.5%, and the antibacterial effect is strong.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 11.67%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 19.86%.

Example 2

The preparation method of the essential oil of spearmint, the pure dew of spearmint, the lavender essential oil, the atractylis lancea essential oil, the wormwood essential oil and the sweet wormwood essential oil in the embodiment is the same as that in the embodiment 1.

mixing 8 parts of propylene glycol with 3 parts of spearmint essential oil, 0.3 part of lavender essential oil, 0.2 part of atractylis lancea essential oil, 0.2 part of wormwood essential oil and 0.2 part of sweet wormwood essential oil in the preparation process to fully dissolve the mixture to prepare a phase A mixed solution; mixing 0.4 part of phenoxyethanol and 0.1 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.1 part of sodium citrate, 0.03 part of bronopol, 207 parts of tween, 0.1 part of sodium hyaluronate and 0.1 part of defoaming agent, and stirring at room temperature for 30min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 60min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 60min, and standing for 24h to obtain antibacterial spray product.

The test of the embodiment is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items of pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are respectively 97.8%, 99.9% and 96.8%, and the test items have strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 11.54%; compared with the basic value, the product has obvious increase (p is less than 0.001) at four weeks of use, and the increase rate is 20.01 percent.

Example 3

mixing 10 parts of propylene glycol with 4 parts of spearmint essential oil, 0.4 part of lavender essential oil, 0.2 part of atractylis lancea essential oil, 0.2 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; mixing 0.5 part of phenoxyethanol and 0.05 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.05 part of sodium citrate, 0.01 part of bronopol, 205 parts of tween, 0.05 part of sodium hyaluronate and 0.1 part of defoaming agent, and stirring at room temperature for 15min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 40min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 40min, and standing for 12h to obtain antibacterial spray product.

In the embodiment, the test is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items of pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 96.5%, 100% and 95.8% respectively, and the test items have strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.23%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 19.78%.

Example 4

mixing 5 parts of propylene glycol with 2 parts of spearmint essential oil, 0.3 part of lavender essential oil, 0.1 part of atractylis lancea essential oil, 0.1 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; mixing 0.5 part of phenoxyethanol and 0.01 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.05 part of sodium citrate, 0.01 part of bronopol, 204 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoaming agent, and stirring at room temperature for 25min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 50min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 50min, and standing for 36h to obtain antibacterial spray product.

In the embodiment, the test is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items of pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are respectively 96.5%, 98.5% and 96.7%, and the test items have strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.17%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 19.53 percent.

Example 5

mixing 10 parts of propylene glycol with 5 parts of spearmint essential oil, 0.3 part of lavender essential oil, 0.2 part of atractylis lancea essential oil, 0.3 part of wormwood essential oil and 0.2 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.05 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.1 part of sodium citrate, 0.02 part of bronopol, 208 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoaming agent, and stirring at room temperature for 30min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 30min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 60min, and standing for 72h to obtain antibacterial spray product.

In the embodiment, the test is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus and candida albicans are respectively 98.6%, 100% and 97.5%, and the test items have strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 11.62%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 21.03%.

Example 6

mixing 10 parts of propylene glycol with 5 parts of spearmint essential oil, 0.4 part of lavender essential oil, 0.1 part of atractylis lancea essential oil, 0.3 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; mixing 0.4 part of phenoxyethanol and 0.05 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.1 part of sodium citrate, 0.02 part of bronopol, 205 parts of tween, 0.05 part of sodium hyaluronate and 0.1 part of defoaming agent, and stirring at room temperature for 5min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 60min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 30min, and standing for 10h to obtain antibacterial spray product.

In the embodiment, the test is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus and candida albicans are respectively 98.8%, 100% and 98.5%, and the test items have strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 11.52%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 20.76%.

Example 7

mixing 9 parts of propylene glycol with 4 parts of spearmint essential oil, 0.3 part of lavender essential oil, 0.1 part of atractylis lancea essential oil, 0.2 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.1 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.05 part of sodium citrate, 0.01 part of bronopol, 208 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoaming agent, and stirring at room temperature for 15min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 40min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 50min, and standing for 8h to obtain antibacterial spray product.

The test of the embodiment is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items of pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 97.6%, 98.8% and 95.8%, respectively, and the test items have strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.86%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 19.78%.

Example 8

mixing 8 parts of propylene glycol with 3 parts of spearmint essential oil, 0.3 part of lavender essential oil, 0.2 part of atractylis lancea essential oil, 0.2 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil in the preparation process to fully dissolve the mixture to prepare a phase A mixed solution; mixing 0.2 part of phenoxyethanol and 0.05 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.1 part of sodium citrate, 0.05 part of bronopol, 206 parts of tween, 0.05 part of sodium hyaluronate and 0.1 part of defoaming agent, and stirring at room temperature for 5min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 35min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 50min, and standing for 1h to obtain antibacterial spray product.

In the embodiment, the test is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items of pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 96.8%, 98.8% and 97.8%, respectively, and the antibacterial test has strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.56%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 19.67%.

Example 9

mixing 7 parts of propylene glycol with 2 parts of spearmint essential oil, 0.5 part of lavender essential oil, 0.1 part of atractylis lancea essential oil, 0.3 part of wormwood essential oil and 0.3 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.05 part of octoxyglycerol with each other to ensure that the phenoxyethanol and the octoxyglycerol are fully dissolved to prepare a B-phase mixed solution; mixing the spearmint hydrosol (the rest) in the preparation process with 0.1 part of sodium citrate, 0.05 part of bronopol, 203 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoaming agent, and stirring at room temperature for 25min to obtain a C-phase mixed solution; mixing the obtained B, C two-phase mixed solution, stirring at room temperature for 45min, slowly adding the obtained A-phase mixed solution into the above solution, stirring for 35min, and standing for 36h to obtain antibacterial spray product.

In the embodiment, the test is carried out according to 2.1.8 antibacterial test in disinfection technical specification, and the result shows that the antibacterial rates of the test items of pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 96.5%, 98.6% and 97.8%, respectively, and the antibacterial test has strong antibacterial action.

In this example, experiments are performed according to the QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the design requirements of the scheme are met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to requirements at a specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.56%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 19.58%.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Comparative example 1 (product 1, based on the formulation of example 1, without Atractylodes lancea essential oil and Artemisia annua essential oil)

The preparation method of the plant essential oil bacteriostatic spray product in the comparative example comprises the following steps (all raw materials are calculated by 100 parts by weight):

mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil and 1 part of wormwood essential oil in the preparation process, and fully dissolving to obtain a phase A mixed solution; the other embodiments are the same as example 1.

The comparative example is tested according to 2.1.8 antibacterial test in 'Disinfection technical Specification', and the results show that the antibacterial rates of the test items of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and the like are 56.8%, 98.9% and 5.3% respectively.

The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the scheme design requirement is met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement at the specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that the adverse reaction record result shows that no adverse reaction occurs, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 8.67%; compared with the basic value, the product has obvious increase (p is less than 0.001) at four weeks of use, and the increase rate is 10.96%.

Comparative example 2 (product 2, based on the formulation of example 1, without lavender essential oil, spearmint essential oil and wormwood essential oil)

mixing 7 parts of propylene glycol with 3.5 parts of atractylis lancea essential oil and 1.5 parts of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare A-phase mixed solution; the other embodiments are the same as example 1.

The comparative example is tested according to 2.1.8 antibacterial test in 'Disinfection technical Specification', and the results show that the antibacterial rates of the test items of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and the like are 4.5%, 98.6% and 58.1% respectively.

The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the scheme design requirement is met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement at the specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that the adverse reaction record result shows that no adverse reaction occurs, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 7.83%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 9.76%.

Comparative example 3 (product 3, based on the formulation of example 1, without Atractylodes lancea essential oil)

mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil, 0.5 part of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; the other embodiments are the same as example 1.

The comparative example is tested according to 2.1.8 antibacterial test in 'Disinfection technical Specification', and the results show that the antibacterial rates of the test items of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and the like are 60.5%, 99.9% and 20.8% respectively.

The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the scheme design requirement is met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement at the specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that the adverse reaction record result shows that no adverse reaction occurs, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 9.39%; compared with the basic value, the product has obvious increase (p is less than 0.001) at four weeks of use, and the increase rate is 10.97%.

Comparative example 4 (product 4, based on the formulation of example 1, without essential oil of Artemisia annua)

mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil, 0.5 part of atractylis lancea essential oil and 0.5 part of wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; the other embodiments are the same as example 1.

The comparative example is tested according to 2.1.8 antibacterial test in 'Disinfection technical Specification', and the results show that the antibacterial rates of the test items of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and the like are 61.1%, 99.9% and 28.5% respectively.

The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the scheme design requirement is met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement at the specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that the adverse reaction record result shows that no adverse reaction occurs, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.58%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 11.93%.

Comparative example 5 (product 5, based on the formulation of example 1, without lavender essential oil)

mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of atractylis lancea essential oil, 0.5 part of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process, and fully dissolving to obtain A-phase mixed solution; the other embodiments are the same as example 1.

The comparative example is tested according to 2.1.8 antibacterial test in 'Disinfection technical Specification', and the results show that the antibacterial rates of the test items of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and the like are 48.6%, 99.9% and 60.1% respectively.

The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the scheme design requirement is met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement at the specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that the adverse reaction record result shows that no adverse reaction occurs, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.10%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 10.56%.

Comparative example 6 (product 6, based on the formulation of example 1, without spearmint essential oil)

mixing 7 parts of propylene glycol with 1.5 parts of lavender essential oil, 1.5 parts of atractylis lancea essential oil, 1.5 parts of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process, and fully dissolving to obtain a phase A mixed solution; the other embodiments are the same as example 1.

The comparative example is tested according to 2.1.8 antibacterial test in 'Disinfection technical Specification', and the results show that the antibacterial rates of the test items of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and the like are 45.8%, 99.9% and 59.5% respectively.

The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the scheme design requirement is met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement at the specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that the adverse reaction record result shows that no adverse reaction occurs, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 9.61%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 11.05%.

Comparative example 7 (product 7, based on the formulation of example 1, without wormwood essential oil)

mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil, 0.5 part of atractylis lancea essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to fully dissolve the materials to prepare a phase A mixed solution; the other embodiments are the same as example 1.

The comparative example is tested according to 2.1.8 antibacterial test in 'Disinfection technical Specification', and the results show that the antibacterial rates of the test items of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and the like are 48.8%, 99.9% and 59.8% respectively.

The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guideline, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the scheme design requirement is met, 32 subjects are 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement at the specified time, the product is continuously used for four weeks according to the cosmetic safety technical specification 2015, on the premise that the adverse reaction record result shows that no adverse reaction occurs, the moisture content of the skin cuticle of the cheek is remarkably increased (p is less than 0.001) compared with the basic value (43%) for one week of the product use, and the increase rate is 10.54%; compared with the basic value, the product has remarkable increase (p is less than 0.001) at four weeks of use, and the increase rate is 11.86%.

The bacteriostatic spray products prepared in comparative examples 1-7 and example 1 are compared with the bacteriostatic spray product of a certain brand in volunteers for trial use, and the experimental results are shown in the following table:

experiments show that the plant essential oil bacteriostatic spray product has a very strong bacteriostatic effect and a very obvious moisturizing effect. In different plant essential oils, the combination of the spearmint essential oil, the lavender essential oil and the wormwood essential oil has a strong inhibiting effect on pseudomonas aeruginosa, the bacteriostasis rate of the single use or the combination of any two essential oils is less than 50%, but the inhibiting effect of the three essential oils on candida albicans is weak; the atractylis lancea essential oil and the artemisia apiacea essential oil have strong bacteriostatic action on candida albicans when being used in a combined way, the bacteriostatic rate is less than 50% when being used singly, and both the two essential oils have weak inhibitory action on pseudomonas aeruginosa; the five essential oils have the strongest inhibition effect on staphylococcus aureus, the inhibition rate can reach more than 90%, and in order that the inhibition rates of three bacteria can reach more than 90%, the five essential oils are required to be used in combination, so that the inhibition effect is better and obvious; the essential oils contain volatile components of organic compounds such as alcohols, aldehydes, acids, phenols, esters, ketones, terpenes and the like, have good effects of resisting oxidation, diminishing inflammation, nursing and moisturizing on human skin, and relieve the skin function, and the moisture content of the skin is higher than that of the single essential oil.

The foregoing shows and describes the fundamental principles and principal features of the invention, together with the advantages and benefits thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The bacteriostatic spray of the plant essential oil comprises A, B, C three-phase components in 100 parts by weight, wherein the A phase comprises the following components: 0.01-5 parts of spearmint essential oil, 0.01-1 part of lavender essential oil, 0.01-1 part of atractylis lancea essential oil, 0.01-1 part of wormwood essential oil, 0.01-1 part of sweet wormwood essential oil and 0.05-10 parts of propylene glycol; phase B: 0.01-1 part of phenoxyethanol and 0.1-0.5 part of octoxyglycerol; and C phase: 0.01-0.1 part of sodium citrate, 0.01-0.05 part of bronopol, 200.5-10 parts of tween, 0.01-0.1 part of sodium hyaluronate, 0.01-0.3 part of defoaming agent and the balance of spearmint hydrosol.

2. The plant essential oil bacteriostatic spray according to claim 1, characterized in that the preparation process of the spearmint essential oil and the spearmint hydrosol is as follows:

weighing spearmint raw materials, placing the spearmint raw materials in a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking for 12-14 h, carrying out oil bath heating at 110 ℃ for 4h to obtain distilled volatile oil, taking out the volatile oil, continuing distilling for 2-3 h to obtain water content of the distilled water, adding a small amount of anhydrous sodium sulfate into the volatile oil for water absorption and drying, filtering to obtain spearmint essential oil, and filtering the distilled water to obtain spearmint hydrosol.

3. The plant essential oil bacteriostatic spray according to claim 1, which is characterized in that: the preparation process of the lavender essential oil is as follows: weighing lavender raw materials, placing the lavender raw materials into a drying container, adding distilled water according to the liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking for 6-8 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain the lavender essential oil.

4. The plant essential oil bacteriostatic spray according to claim 1, which is characterized in that: the preparation process of the atractylis lancea essential oil is as follows: weighing Atractylodes lancea raw material, placing into a drying container, adding distilled water according to a liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking for 10-12 h, heating in an oil bath at 110 ℃ for extraction for 6h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain Atractylodes lancea essential oil.

5. The plant essential oil bacteriostatic spray according to claim 1, which is characterized in that: the preparation process of the wormwood essential oil is as follows: weighing folium Artemisiae Argyi raw materials, placing in a drying container, adding distilled water at a liquid-material ratio of 12:1 (ml/g) by conventional steam distillation method, soaking for 12-16 h, heating in 110 deg.C oil bath for extraction for 4h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, water-absorbing, drying, and filtering to obtain folium Artemisiae Argyi essential oil.

6. The plant essential oil bacteriostatic spray according to claim 1, which is characterized in that: the preparation process of the sweet wormwood essential oil is as follows: weighing sweet wormwood raw materials, placing the sweet wormwood raw materials into a drying container, adding distilled water according to the liquid-material ratio of 10:1 (ml/g) by using a traditional steam distillation method, soaking for 8-12 h, heating and extracting for 4h by using an oil bath at 110 ℃ to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil, absorbing water, drying, and filtering to obtain sweet wormwood essential oil.

7. A preparation method of a plant essential oil bacteriostatic spray according to any one of claims 1 to 6, characterized by comprising the following steps:

(1) mixing propylene glycol with spearmint essential oil, lavender essential oil, atractylis lancea essential oil, wormwood essential oil and sweet wormwood essential oil in parts by weight, and fully dissolving to obtain A-phase mixed solution;

(3) filtering and removing impurities from spearmint hydrosol to obtain a clarified solution, mixing the clarified solution with sodium citrate, bronopol, tween 20, sodium hyaluronate and a defoaming agent in parts by weight, and stirring at room temperature for 5-30min to obtain a C-phase mixed solution;

(4) and (3) mixing the phase B mixed solution obtained in the step (2) and the phase C mixed solution obtained in the step (3) with each other, stirring at room temperature for 30-60min, slowly adding the phase A mixed solution obtained in the step (1), stirring for 30-60min, and standing for 1-72h to obtain the antibacterial spray.

8. The plant essential oil bacteriostatic spray according to claims 1-6 is applied to skin and mucosa sanitation, has no stimulation to skin, has good system stability, and has the functions of bacteriostasis, moisture preservation, inflammation diminishing, nerve soothing and skin care.