WO2021179567A1 - Chinese mugwort leaf composite essential oil for relieving sore throat and preparation method therefor - Google Patents

Abstract

Disclosed in the present invention are Chinese mugwort leaf composite essential oil for relieving sore throat and a preparation method therefor. The composite essential oil for relieving sore throat comprises the following components in parts by mass: 0.1-5.0 parts of artemisia verlotorum leaf essential oil, 0.1-3.0 parts of lemon essential oil, 0.1-2.0 parts of tea tree essential oil, 0.1-2.0 parts of lavender essential oil, 0.1-6.0 parts of rosemary essential oil, 15.0-25.0 parts of jojoba oil, 15.0-25.0 parts of sweet almond oil and 30.0-60.0 parts of camellia seed oil. According to the composite essential oil, different plant essential oils are reasonably blended to synergistically exert efficacy of the essential oils, and the essential oils are organically combined with high-permeability base oil such as camellia oil and jojoba oil, so that the composite essential oil can effectively permeate the skin, and has effects of effectively resisting bacteria, diminishing inflammation, relieving cough, and relieving sore throat on symptoms such as throat inflammation, cough, swelling and pain caused by pathogenic bacteria. In addition, the composite essential oil is natural, safe and nontoxic, and has fragrant smell, stable quality and a long shelf life.

Description

Mugwort compound essential oil for relieving sore throat and preparation method thereof Technical field

The invention relates to the technical field of essential oils, in particular to a wormwood compound essential oil for relieving sore throat and a preparation method thereof.

Background technique

Sore throat is one of the most common symptoms. Inflammatory reactions such as colds, sinusitis, whooping cough, tonsillitis, pharyngitis, and viral infections can cause sore throat. There are many bacteria that can cause sore throat inflammation, among which Haemophilus influenzae, Streptococcus pyogenes, Haemophilus parainfluenzae, Streptococcus pneumoniae, Mycobacterium tuberculosis, Staphylococcus aureus, etc. are the main bacteria. If throat pain is not treated for a long time, it may even cause rheumatic fever, which can harm the heart and kidneys. At present, the main drugs used to treat sore throat are anti-inflammatory drugs and antibiotics. However, many anti-inflammatory drugs are easy to cause gastrointestinal damage during the digestion and absorption process in the body. Frequent use of antibiotics is likely to make bacteria resistant, and the state restricts their use. Therefore, the research and development of a safe and effective product for relieving and treating throat pain is of great practical value.

Plant essential oils are natural volatile components of plants prepared by steam distillation or other methods. The main components are terpenoids such as monoterpenes, sesquiterpenes, diterpenes, and their oxygen-containing derivatives, and low molecular weight alcohols, aldehydes and ketones. Class and other substances. A variety of ingredients in plant essential oils have anti-bacterial, anti-inflammatory, analgesic and soothing effects, and can be used for anti-inflammatory and analgesic effects of the respiratory tract. Among them, eucalyptol has a variety of therapeutic effects, such as analgesia, anti-inflammatory, and antibacterial. It can be used to treat several respiratory diseases such as sinusitis, asthma, acute bronchitis and chronic bronchitis. Borneol (borneol) is an important component of traditional Asian medicines. It is an effective anti-inflammatory and analgesic. It is mainly used for external treatment and in traditional Chinese medicine for digestive system and respiratory diseases. Terpinenol has the effect of relieving cough, relieving asthma and expectorating. Caryophyllene and oxycaryophyllene have significant central and peripheral nerve analgesia and promote skin penetration. Plant essential oils mostly have plant aromas, which can make people feel happy and have natural advantages in relieving pain.

Each plant essential oil has different biological effects. Mixing different plant essential oils according to the ratio can exert a synergistic effect among essential oils. Using plant essential oils alone is irritating to the skin, easily causing skin burns and redness, and plant essential oils are highly volatile, and must be diluted with other base oils to prepare compound essential oils for use. The base oil can not only prevent the volatilization and oxidation of the essential oil, but also play a synergistic effect on the absorption and efficacy of the essential oil.

Summary of the invention

The purpose of the present invention is to provide a complex essential oil of mugwort leaf for relieving sore throat in response to the defects or deficiencies in the prior art. The compound essential oil is natural, safe, non-toxic, non-polluting, does not contain preservatives, and can effectively relieve sore throat.

The object of the present invention is also to provide a method for preparing the above-mentioned wormwood compound essential oil for relieving throat pain.

A compound essential oil of mugwort leaf for relieving sore throat, in parts by mass, including the following components:

0.1 to 5.0 parts of red wormwood essential oil, 0.1 to 3.0 parts of lemon essential oil, 0.1 to 2.0 parts of tea tree essential oil, 0.1 to 2.0 parts of lavender essential oil, 0.1 to 6.0 parts of rosemary essential oil, 15.0 to 25.0 parts of jojoba oil, sweet almonds 15.0-25.0 parts of oil and 30.0-60.0 parts of camellia seed oil.

In a preferred embodiment, the following components are included in parts by mass:

Red foot wormwood essential oil 0.1～4.0 parts, lemon essential oil 0.4～1.6 parts, tea tree essential oil 0.2～0.5 parts, lavender essential oil 0.2～1.0 parts, rosemary essential oil 1.5～6.0 parts, jojoba oil 20.0～25.0 parts, sweet almond 20.0-25.0 parts of oil and 42.0-57.0 parts of camellia seed oil.

In a preferred embodiment, the following components are included in parts by mass:

Red wormwood essential oil 2.0 parts, lemon essential oil 0.8 parts, tea tree essential oil 0.2 parts, lavender essential oil 0.5 parts, rosemary essential oil 1.5 parts, jojoba oil 20.0 parts, sweet almond oil 20.0 parts, and camellia seed oil 55.0 parts.

In a preferred embodiment, the content of the red wormwood essential oil is 0.1-5.5% based on the mass percentage of the wormwood compound essential oil that relieves sore throat.

In a preferred embodiment, the red wormwood essential oil is obtained by the following method:

The dried wormwood leaves are taken, crushed, and _{extracted with supercritical CO 2 to} separate the wormwood leaves extract; then molecularly distilled to obtain the crude oil of wormwood leaves, stand still, and filter to obtain the wormwood essential oil.

The dried red wormwood leaves are dried leaves of fresh red wormwood after harvesting and removing impurities.

In a more preferred embodiment, the _{temperature of the supercritical CO 2} extraction is 30-60° C., the extraction pressure is 10-15 MPa, and the extraction time is 1 to 3 hours.

In a more preferred embodiment, the separation temperature is 30-60°C, and the separation pressure is 4-7 MPa.

In a more preferred embodiment, the molecular distillation temperature is 90-120°C, the vacuum degree is 50-200 Pa, the internal condensation temperature is 50-70°C, and the cold trap temperature is -15-0°C.

In a preferred embodiment, the lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil are obtained by the following methods:

Put the fresh lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, and extract by steam distillation to collect the primary essential oils, and then fumigate the primary essential oils with water-proof distillation to collect the finished essential oils.

In a more preferred embodiment, the steam distillation time is 2 to 4 hours.

In a more preferred embodiment, the time for the water-proof distillation and fumigation is 2 to 4 hours.

The functional principle of the present invention is:

Red-footed mugwort belongs to Artemisia.verlotorum Lamotte (Artemisia.verlotorum Lamotte) in taxonomy, also known as Baogu'ai. Its botanical characteristics are that the roots and stems are red or reddish brown in a specific period. _{As the supercritical CO 2} extraction product of Artemisia argyi leaf, the main components of Artemisia argyi essential oil are borneol, camphor, eucalyptol, caryophyllene and caryophyllene oxide. These components have significant antibacterial, anti-inflammatory and antitussive properties. Relieve depression, anxiety and relieve nerve pain. Based on the composition of wormwood essential oil, wormwood essential oil can warm meridians, activate collaterals, anti-bacterial and anti-inflammatory, relieve cough and reduce phlegm, relieve pain and calm, promote circulation and improve immunity, prevent and relieve chronic bronchitis, emphysema, bronchial asthma and other respiratory systems Sore and swollen throat caused. However, the traditional wormwood essential oil is mainly extracted from wormwood and other wormwood species by steam distillation. Its essential oil component contains a large amount of thujone, which is neurotoxic, and excessive use may cause kidney failure and cause spasms, etc. ; Moreover, when thujone is applied to the skin, it can cause severe skin redness and burns. Therefore, the compatible red wormwood essential oil in the compound essential oil of the present invention is the wormwood essential oil extracted from the red wormwood by the supercritical and molecular distillation method, which not only retains the original effect of the wormwood essential oil, but also is safer and more effective.

The main component of lemon essential oil is limonene, which has good expectorant and asthmatic, anti-oxidant, anti-inflammatory, anti-tumor, dissolving gallstone, antibacterial and other effects. Moreover, lemon essential oil has many functions, especially in terms of antiseptic, antibacterial and deodorizing. Lemon essential oil is also called a super purifier. Therefore, the combination of lemon essential oil in the compound essential oil of the present invention not only effectively enhances the effect of relieving sore throat, but also has a natural antiseptic effect, so that the compound essential oil remains natural without preservatives.

The main components of rosemary essential oil include borneol and its esters, camphor, campene, eucalyptol and sesquiterpenoids. It has antibacterial, anti-inflammatory, anti-oxidant and anti-cancer effects, and is very beneficial to the respiratory system; Moreover, rosemary essential oil can be used for respiratory diseases such as colds and bronchitis; at the same time, rosemary also has a good antiseptic effect. Compatible with rosemary essential oil in the compound essential oil of the present invention can effectively synergistically enhance the effect of relieving sore throat, and can synergistically enhance the antiseptic effect of the compound essential oil.

The main component of lavender essential oil is linalool, which has significant plant natural aroma components. It can be used as flavoring and deodorant for flavors and fragrances. Foreign folks have used linalool-containing volatile oils or plants as hypnotic and tranquilizers since ancient times. Reports. Moreover, lavender essential oil has good antibacterial activity against Escherichia coli, Enteritidis, Staphylococcus, Saccharomyces cerevisiae and Aspergillus niger. In addition, lavender essential oil can be combined with a variety of pure plant essential oils, and its active factors complement each other, quickly penetrate, and achieve the purpose of disinfection and antibacterial. Compounding lavender essential oil in the compound essential oil of the present invention can promote the compatibility and synergy between the essential oil components, and at the same time can effectively enhance the antiviral effect of the compound essential oil and enhance the sore throat effect.

Tea tree essential oil is extracted from Australian tea tree, and its main component is 4-terpineol. Tea tree essential oil has good broad-spectrum bactericidal, anti-bacterial and health-care effects. It can resist viruses, oxidation, inflammation, parasites, and promote wound healing. It is an excellent natural antibacterial agent that can replace antibiotics. The combination of tea tree essential oil in the compound essential oil of the present invention can effectively enhance the bactericidal and antiviral effects of the compound essential oil, thereby effectively enhancing the effect of relieving sore throat.

Camellia seed oil is rich in glycerolipids, which is a curative and health-care nutrient oil, which can be fully integrated with human skin and absorbed by the surface of the body. It has the functions of moisturizing, moisturizing, hair care and beauty. Jojoba oil is the most permeable base oil, which is easily absorbed by the skin. It can carry plant essential oils to quickly penetrate the skin to reach the areas where it needs to be effective. At the same time, jojoba oil can refresh and remove greasy and effectively improve oily skin. The best skin moisturizing oil. Sweet almond oil is rich in fatty acids, protein, and vitamins A, B1, B2, B6, and E. It can improve skin redness, swelling, dryness and inflammation, and has the effect of moisturizing and softening the skin. In the compound essential oil of the present invention, the compound of camellia seed oil, jojoba oil and sweet almond oil is used as the base oil, which not only improves the synergistic effect of the essential oil components, but also improves the interaction with human skin The adaptability makes the compound essential oil easier to be absorbed by the skin quickly, has a faster and better curative effect, is gentle and non-irritating, and has the effect of caring for the skin.

The method for preparing the wormwood compound essential oil for relieving sore throat as described in any one of the above includes the following steps:

According to the mass parts, mix the red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil evenly to obtain the relief of sore throat Mugwort complex essential oil.

The wormwood compound essential oil for relieving throat pain of the present invention is mixed with red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia seed oil according to the stated mass parts , Coordinated with each other, complemented each other, synergistic and synergistic, to achieve the effect of relieving throat pain. In addition, after mixing, the components in the argyle leaf compound essential oil of the present invention are fully coordinated and mixed uniformly, and no phenomena such as stratification, precipitation, etc. occur.

The wormwood compound essential oil for relieving sore throat of the present invention is used to apply an appropriate amount for external application to the swelling and sore throat.

Compared with the prior art, the present invention has the following advantages and beneficial effects:

(1) The wormwood compound essential oil for relieving sore throat of the present invention is made of red foot wormwood essential oil in combination with lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia seed oil. Aromatic smell not only has the effect of pleasant mood and relieve stress, but also can effectively inhibit and kill the bacteria that cause throat pain and inflammation, relieve throat inflammation, and effectively relieve throat pain.

(2) In the argyle leaf compound essential oil for relieving throat pain of the present invention, the compounded essential oil components are all natural sources, safe, non-polluting, and contain no preservatives, each component is synergistic, has good compatibility, and the system Stable, strong natural antiseptic ability, long shelf life.

(3) In the wormwood compound essential oil for relieving throat pain of the present invention, the essential oil of wormwood is extracted from wormwood by supercritical carbon dioxide fluid extraction technology and molecular distillation, and lemon essential oil, lavender essential oil, tea tree essential oil, rosemary The essential oil is obtained by steam distillation, which has low acquisition cost, can guarantee the quality of the product, and the steps are simple to operate, and the equipment used is all conventional equipment, which is easy to realize industrialized production.

Description of the drawings

Fig. 1 is the composition spectrum of the essential oil of the red foot wormwood extracted by supercritical and molecular distillation in Example 1.

Detailed ways

The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments and drawings. It should be noted here that the description of these embodiments is only used to help understand the present invention, and the protection scope and implementation modes of the present invention are not limited to this. In addition, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. And, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments, and are not intended to limit the present invention.

Example 1

The wormwood compound essential oil for relieving sore throat of this embodiment, in parts by mass, includes the following components:

Red wormwood essential oil 2 parts, lemon essential oil 0.8 parts, tea tree essential oil 0.2 parts, lavender essential oil 0.5 parts, rosemary essential oil 1.5 parts, jojoba oil 20 parts, sweet almond oil 20 parts, camellia seed oil 55 parts.

Among them, the essential oil of wormwood in this embodiment is obtained by the following method:

(1) The dried red mugwort leaves are crushed and loaded into a supercritical CO ₂ extraction device for extraction. The supercritical extraction temperature is adjusted to 50°C, the extraction pressure is 13MPa, and the extraction time is 3h; after the extraction is completed, it is passed through the separation kettle Separation, wherein the separation temperature of the separation kettle is 40°C, and the separation pressure is 6MPa; the extract is dissolved at 70°C and then condensed to remove water to obtain mugwort extract.

(2) Mugwort extract is heated to 80℃ to dissolve and then enter the molecular distillation equipment. Set the distillation temperature of molecular distillation to 90℃, vacuum degree to 100Pa, internal condensation temperature to 50℃, cold trap temperature to -5～0℃, and separate , Collect the wormwood light oil in the cold trap; let the wormwood light oil stand and filter to obtain the red wormwood essential oil.

The prepared wormwood essential oil was analyzed by liquid chromatograph. The analyzed component spectrum is shown in Figure 1. The main components and relative contents of the wormwood essential oil after sorting are shown in Table 1.

Table 1 The main components and relative contents of the essential oil of red foot wormwood (area normalization method)

Element	Relative content (%)
Borneol	34.1
Caryophyllene	11.6
Caryophyllene oxide	11.7
Eucalyptus	4.1
Camphor	6.5
Thujone	0.5
total	68.5

From the analysis of Figure 1 and Table 1, it can be seen that the main components of the essential oil of redfoot wormwood are borneol, camphor, eucalyptol, caryophyllene and caryophyllene oxide, and these components have significant antibacterial, anti-inflammatory, antitussive, and alleviating effects. Depression, anxiety and relieve nerve pain.

The lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil in this embodiment are obtained by the following methods:

Put the lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, and extract by steam distillation for 4 hours, collect the primary essential oils, and then fumigate the primary essential oils by water distillation for 2 hours to collect the finished essential oils.

The method for preparing the wormwood compound essential oil for relieving throat pain of this embodiment includes the following steps:

According to the said mass parts, the said red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil are evenly mixed at room temperature to obtain the said Mugwort complex essential oil that soothes sore throat.

The prepared argyle leaf compound essential oil is evenly mixed, and does not appear to be stratified or precipitated.

Example 2

The wormwood compound essential oil for relieving sore throat of this embodiment, in parts by mass, includes the following components:

4 parts of mugwort essential oil, 0.4 parts of lemon essential oil, 0.4 parts of tea tree essential oil, 0.2 parts of lavender essential oil, 1.5 parts of rosemary essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, and 57 parts of camellia seed oil.

Among them, the essential oil of wormwood in this embodiment is obtained by the following method:

(1) The dried red mugwort leaves are crushed and loaded into a supercritical CO ₂ extraction device for extraction. The supercritical extraction temperature is adjusted to 60℃, the extraction pressure is 10MPa, and the extraction time is 2h; after the extraction is completed, it is passed through the separation kettle Separation, wherein the separation temperature of the separation kettle is 30°C, and the separation pressure is 4MPa; the extract is dissolved at 70°C and water is removed to obtain a mugwort extract.

(2) Mugwort extract is heated to 80℃ to dissolve and then enter the molecular distillation equipment. Set the distillation temperature of molecular distillation to 120℃, vacuum degree of 50Pa, internal condensation temperature of 70℃, and cold trap temperature of -15～-5℃. Separate, obtain the wormwood light oil; let the wormwood light oil stand and filter to obtain the red foot wormwood essential oil.

The main component analysis spectrum and composition table of the prepared red foot wormwood essential oil are shown in Figure 1 and Table 1, respectively. The main components of the prepared red foot wormwood essential oil are borneol, camphor, eucalyptol, caryophyllene and oxidized caryophyllum These ingredients have significant antibacterial, anti-inflammatory, antitussive, relieving depression, anxiety, and nerve pain.

The lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil in this example were obtained by the following methods:

Put the lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, and extract the primary essential oils by steam distillation for 3 hours, collect the primary essential oils, and then fumigate the primary essential oils by water distillation for 3 hours to collect the finished essential oils.

The method for preparing the wormwood compound essential oil for relieving throat pain of this embodiment includes the following steps:

According to the said mass parts, the said red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil are evenly mixed at room temperature to obtain the said Mugwort complex essential oil that soothes sore throat.

The prepared argyle leaf compound essential oil is evenly mixed, and does not appear to be stratified or precipitated.

Example 3

The wormwood compound essential oil for relieving sore throat of this embodiment, in parts by mass, includes the following components:

0.1 part of mugwort essential oil, 0.1 part of lemon essential oil, 0.1 part of tea tree essential oil, 2.0 parts of lavender essential oil, 4.0 parts of rosemary essential oil, 15 parts of jojoba oil, 15 parts of sweet almond oil, and 60 parts of camellia seed oil.

Among them, the essential oil of wormwood in this embodiment is obtained by the following method:

(1) The dried red mugwort leaves are crushed and loaded into a supercritical CO ₂ extraction device for extraction. The supercritical extraction temperature is adjusted to 45°C, the extraction pressure is 12MPa, and the extraction time is 1h; after the extraction is completed, it is passed through the separation kettle Separation, wherein the separation temperature of the separation kettle is 50°C, and the separation pressure is 5MPa; the extract is dissolved at 70°C and water is removed to obtain a mugwort extract.

(2) Mugwort extract is heated to 80°C to dissolve, and then enter the molecular distillation equipment. Set the distillation temperature of molecular distillation to 105°C, the vacuum degree to 150Pa, the internal condensation temperature to 60°C, and the cold trap temperature to -10~-2°C. The wormwood light oil is separated and the wormwood extract is molecularly distilled; the wormwood light oil is allowed to stand and filtered to obtain the red wormwood essential oil.

The main component analysis spectrum and composition table of the prepared red foot wormwood essential oil are shown in Figure 1 and Table 1, respectively. The main components of the prepared red foot wormwood essential oil are borneol, camphor, eucalyptol, caryophyllene and oxidized caryophyllum These ingredients have significant antibacterial, anti-inflammatory, antitussive, relieving depression, anxiety, and nerve pain.

The lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil in this embodiment are obtained by the following methods:

Put the lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, and extract the primary essential oils by steam distillation for 3 hours, collect the primary essential oils, and then fumigate the primary essential oils with water distillation for 2 hours to collect the finished essential oils.

The method for preparing the wormwood compound essential oil for relieving throat pain of this embodiment includes the following steps:

According to the said mass parts, the said red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil are evenly mixed at room temperature to obtain the said Mugwort complex essential oil that soothes sore throat.

The prepared argyle leaf compound essential oil is evenly mixed, and does not appear to be stratified or precipitated.

Example 4

The wormwood compound essential oil for relieving sore throat of this embodiment, in parts by mass, includes the following components:

0.5 part of mugwort essential oil, 0.8 part of lemon essential oil, 0.5 part of tea tree essential oil, 0.2 part of lavender essential oil, 6 parts of rosemary essential oil, 25 parts of jojoba oil, 25 parts of sweet almond oil, and 42 parts of camellia seed oil.

Among them, the essential oil of wormwood in this embodiment is obtained by the following method:

(1) The dried red mugwort leaves are crushed and loaded into a supercritical CO ₂ extraction device for extraction. The supercritical extraction temperature is adjusted to 30℃, the extraction pressure is 15MPa, and the extraction time is 1h; after the extraction is completed, it is passed through the separation kettle Separation; Among them, the separation temperature of the separation kettle is 40°C, and the separation pressure is 7MPa; the extract is dissolved at 70°C and water is removed to obtain a mugwort extract.

(2) Mugwort extract is heated to 80°C to dissolve and then enter the molecular distillation equipment. Set the distillation temperature of molecular distillation to 100°C, vacuum degree to 200Pa, internal condensation temperature to 60°C, cold trap temperature to -5～0°C, and separate The obtained wormwood light oil wormwood extract is molecularly distilled; the wormwood light oil is allowed to stand and filtered to obtain the red wormwood essential oil.

The main component analysis spectrum and composition table of the prepared red foot wormwood essential oil are shown in Figure 1 and Table 1, respectively. The main components of the prepared red foot wormwood essential oil are borneol, camphor, eucalyptol, caryophyllene and oxidized caryophyllum These ingredients have significant antibacterial, anti-inflammatory, antitussive, relieving depression, anxiety, and nerve pain.

The lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil in this embodiment are obtained by the following methods:

Put the lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, and extract the primary essential oils by steam distillation for 2 hours, collect the primary essential oils, and then fumigate the primary essential oils by water-proof distillation for 2 hours to collect the finished essential oils.

The method for preparing the wormwood compound essential oil for relieving throat pain of this embodiment includes the following steps:

According to the said mass parts, the said red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil are evenly mixed at room temperature to obtain the said Mugwort complex essential oil that soothes sore throat.

The prepared argyle leaf compound essential oil is evenly mixed, and does not appear to be stratified or precipitated.

Example 5

The wormwood compound essential oil for relieving sore throat of this embodiment, in parts by mass, includes the following components:

0.1 part of mugwort essential oil, 1.6 parts of lemon essential oil, 0.4 part of tea tree essential oil, 1 part of lavender essential oil, 1.9 parts of rosemary essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, and 55 parts of camellia seed oil.

Among them, the essential oil of wormwood in this embodiment is obtained by the following method:

(1) The dried red mugwort leaves are crushed and loaded into a supercritical CO ₂ extraction device for extraction. The supercritical extraction temperature is adjusted to 50℃, the extraction pressure is 15MPa, and the extraction time is 2h; after the extraction is completed, it is passed through the separation kettle Separation; Among them, the separation temperature of the separation kettle is 30°C, and the separation pressure is 4MPa; the extract is dissolved at 70°C, and water is removed to obtain a mugwort extract.

(2) Mugwort extract is heated to 80°C to dissolve and then enter the molecular distillation equipment. Set the distillation temperature of molecular distillation to 90°C, vacuum degree to 150Pa, internal condensation temperature to 60°C, cold trap temperature to -5～0°C, and separate Obtain the wormwood light oil; let the wormwood light oil stand and filter to obtain the red wormwood essential oil.

The main component analysis spectrum and composition table of the prepared red foot wormwood essential oil are shown in Figure 1 and Table 1, respectively. The main components of the prepared red foot wormwood essential oil are borneol, camphor, eucalyptol, caryophyllene and oxidized caryophyllum These ingredients have significant antibacterial, anti-inflammatory, antitussive, relieving depression, anxiety, and nerve pain.

The lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil in this embodiment are obtained by the following methods:

Put the lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, extract by steam distillation for 4 hours, collect the primary essential oils, and then fumigate the primary essential oils by water distillation for 3 hours to collect the finished essential oils.

The method for preparing the wormwood compound essential oil for relieving throat pain of this embodiment includes the following steps:

According to the said mass parts, the said red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil are evenly mixed at room temperature to obtain the said Mugwort complex essential oil that soothes sore throat.

The prepared argyle leaf compound essential oil is evenly mixed, and does not appear to be stratified or precipitated.

Example 6

The wormwood compound essential oil for relieving sore throat of this embodiment, in parts by mass, includes the following components:

5.0 parts of mugwort essential oil, 3.0 parts of lemon essential oil, 2.0 parts of tea tree essential oil, 0.1 part of lavender essential oil, 0.1 part of rosemary essential oil, 25 parts of jojoba oil, 25 parts of sweet almond oil, and 30 parts of camellia seed oil.

Among them, the essential oil of wormwood in this embodiment is obtained by the following method:

(1) The dried red mugwort leaves are crushed and loaded into a supercritical CO ₂ extraction device for extraction. The supercritical extraction temperature is adjusted to 55℃, the extraction pressure is 12MPa, and the extraction time is 2h; after the extraction is completed, it is passed through the separation kettle Separation; Among them, the separation temperature of the separation kettle is 60°C, and the separation pressure is 6MPa; the extract is dissolved at 70°C, and water is removed to obtain a mugwort extract.

(2) The mugwort extract is heated to 80°C to dissolve and then enters the molecular distillation equipment. Set the distillation temperature of molecular distillation to 120°C, the vacuum degree to 100Pa, the internal condensation temperature to 50°C, and the cold trap temperature to -15～-5°C. The wormwood light oil is separated and the wormwood extract is molecularly distilled; the wormwood light oil is allowed to stand and filtered to obtain the red wormwood essential oil.

The main component analysis spectrum and composition table of the prepared red foot wormwood essential oil are shown in Figure 1 and Table 1, respectively. The main components of the prepared red foot wormwood essential oil are borneol, camphor, eucalyptol, caryophyllene and oxidized caryophyllum These ingredients have significant antibacterial, anti-inflammatory, antitussive, relieving depression, anxiety, and nerve pain.

The lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil in this embodiment are obtained by the following methods:

Put the lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, and extract the primary essential oils by steam distillation for 3 hours, collect the primary essential oils, and then fumigate the primary essential oils by water-proof distillation for 4 hours to collect the finished essential oils.

The method for preparing the wormwood compound essential oil for relieving throat pain of this embodiment includes the following steps:

According to the said mass parts, the said red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil are evenly mixed at room temperature to obtain the said Mugwort complex essential oil that soothes sore throat.

The prepared argyle leaf compound essential oil is evenly mixed, and does not appear to be stratified or precipitated.

Comparative example 1～5

The composite essential oils of mugwort leaves of Comparative Examples 1 to 5 were prepared according to the following formula:

Comparative Example 1: 0.8 parts of lemon essential oil, 0.2 parts of tea tree essential oil, 0.5 parts of lavender essential oil, 1.5 parts of rosemary essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, and 57 parts of camellia seed oil.

Comparative example 2: 2 parts of red wormwood essential oil, 0.2 parts of tea tree essential oil, 0.5 parts of lavender essential oil, 1.5 parts of rosemary essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, and 55.8 parts of camellia seed oil.

Comparative Example 3: 2 parts of red foot wormwood essential oil, 0.8 parts of lemon essential oil, 0.5 parts of lavender essential oil, 1.5 parts of rosemary essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, and 55.2 parts of camellia seed oil.

Comparative Example 4: 2 parts of red wormwood essential oil, 0.8 parts of lemon essential oil, 0.2 parts of tea tree essential oil, 1.5 parts of rosemary essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, and 55.5 parts of camellia seed oil.

Comparative Example 5: 2 parts of red wormwood essential oil, 0.8 parts of lemon essential oil, 0.2 parts of tea tree essential oil, 0.5 parts of lavender essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, and 56.5 parts of camellia seed oil.

Refer to Example 1 for the preparation method and process of the wormwood compound essential oil of Comparative Examples 1 to 5.

Test case 1

The wormwood compound essential oils prepared in Examples 1 to 6 and Comparative Examples 1 to 5 were subjected to antibacterial experiments. The experimental process is as follows:

(1) Test strains: Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae. The three activated strains are prepared into a bacterial suspension with a culture medium for later use.

(2) Treatment of Mugwort compound essential oil: Use a sterile filter paper with a diameter of 6mm to fully infiltrate the Mugwort compound essential oil.

(3) Take 0.1ml of bacterial suspension and pour them into petri dishes for plate culture. Place sterile filter paper infused with Mugwort compound essential oil in each petri dish to use bacteria-containing petri dishes without filter paper. As a control, the above-mentioned plate was finally cultured in a constant temperature incubator, and finally the diameter of the inhibition zone was measured.

In the petri dish containing Mugwort compound essential oil prepared above, if the diameter of the inhibition zone is less than or equal to 6mm, it is judged as no antibacterial effect, and the mark is "-"; if the diameter of the inhibition zone is 6-9mm, it is judged that the antibacterial effect is weak, mark It is "±"; those with an inhibition zone diameter of 9-12mm are judged to have moderate bacteriostasis, marked as "+"; those with an inhibition zone diameter of 12-15mm are judged to have strong bacteriostasis; marked as "++" Those with the diameter of the inhibition zone ≥ 15mm are judged to have strong bacteriostasis and marked as "+++".

Table 2 shows the antibacterial results of the wormwood compound essential oils of Examples 1 to 6 and Comparative Examples 1 to 5.

Table 2 Antibacterial test results of the Mugwort Compound Fund of Examples 1 to 6 and Comparative Examples 1 to 5

From the test structure in Table 2, it can be seen that the different proportions of Mugwort leaf compound essential oil have a certain degree of antibacterial effect. Among them, the groups of Examples 1 to 6 are similar and have strong bacteriostatic effects on three kinds of bacteria. The bacteriostatic effects of Example 3 and Examples 5 to 6 are slightly lower than that of Examples 1-2 and 4. The bactericidal effect of Examples 1 to 6 is better than that of Comparative Examples 1 to 5, which means that the essential oils of Artemisia wormwood, lemon essential oil, tea tree essential oil, lavender essential oil, and rosemary essential oils in Examples 1 to 6 have synergistic bactericidal effects, especially Targets bacteria that cause sore throat. Moreover, the weight of the five essential oils is controlled to 2 parts of red wormwood essential oil, 0.8 parts of lemon essential oil, 0.2 parts of tea tree essential oil, 0.5 parts of lavender essential oil, and 1.5 parts of rosemary essential oil, which can maximize the synergistic effect and make it The respective effects are further improved, and the antibacterial effect is better.

Test case 2

The wormwood compound essential oils prepared in Examples 1 to 6 and Comparative Examples 1 to 5 were tested for the anti-inflammatory effect of RAW264.7 cells induced by LPS in vitro. The anti-inflammatory model of mouse macrophages RAW264.7 induced by bacterial lipopolysaccharide (LPS) is a commonly used in vitro anti-inflammatory model. RAW264.7 cells secrete and produce a large amount of nitric oxide (NO) under the induction of LPS, and its content can be used as an indicator of the degree of inflammatory response. In medicine, anti-inflammatory drugs can be preliminarily screened by measuring the inhibition rate of NO secretion in RAW264.7 cells.

The specific process of this experiment is as follows:

(1) The purchased mouse mononuclear macrophage RAW264.7 cells were resuscitated and subcultured.

(2) The wormwood compound essential oil and LPS prepared in Examples 1 to 6 and Comparative Examples 1 to 5 were formulated into 100 μg/ml and 1 μg/ml, respectively, and added to the culture medium for cell culture of RAW264.7, and the growth pair was taken The number of cells in several phases was determined by MTT colorimetry and hemocytometer to determine the number of RAW264.7 viable cells. The blank control group was added with the same amount of normal saline.

Calculate the survival rate of each group of cells:

In the formula: CV represents cell survival rate;

V1 represents the survival rate of cells in the experimental group;

V0 represents the cell survival rate of the blank control group

(3) According to the concentration of 100μg/ml, the wormwood compound essential oils prepared in Examples 1 to 6 and Comparative Examples 1 to 5 were mixed with 1μg/ml LPS and then added to the culture medium for cell culture, LPS control group In order not to add the Mugwort compound essential oil group, the blank control was added with the same amount of normal saline.

(4) The Griess method was used to measure the NO secretion of RAW264.7 cells in each experimental group, and calculate the inhibition rate of NO secretion.

In the formula: NR represents the inhibition rate of NO secretion;

C1 represents the amount of NO produced in each experimental group;

C0 represents the amount of NO produced in the blank control group;

S1 represents the amount of NO produced in the LPS control group.

The results of the in vitro anti-inflammatory effect test are shown in Table 3 below.

Table 3 Test results of each experimental group on the survival rate of RAW264.7 cells and the inhibition of NO secretion

Group	CV(%)	NR(%)
Example 1	104.5	53.4
Example 2	103.9	49.2
Example 3	101.2	47.8
Example 4	101.3	45.4
Example 5	102.8	48.9
Example 6	101.3	48.3
Comparative example 1	101.4	28.9

Comparative example 2	104.1	41.4
Comparative example 3	102.7	23.3
Comparative example 4	103.6	40.6
Comparative example 5	100.3	36.5
Blank control	100.0	0.0
LSP control group	101.7	-

From the experimental results in Table 3, it can be seen that the 100μg/ml Mugwort complex essential oil and 1μg/ml LPS will not cause cell death, and even slightly stimulate growth, but the effect is not significant. This shows that Mugwort leaf compound essential oil at this concentration and below is safe for cells and has no toxic side effects. The inhibition rate of NO secretion indicates that the wormwood compound essential oil has anti-inflammatory effects on LPS-induced inflammation of RAW264.7 cells, among which the anti-inflammatory effects of Examples 1 to 6 are slightly better than those of Comparative Examples 1 to 5, and the wormwood compound essential oil ratio is red foot wormwood essential oil. 2 parts, 0.8 parts of lemon essential oil, 0.2 parts of tea tree essential oil, 0.5 parts of lavender essential oil, 1.5 parts of rosemary essential oil, 20 parts of jojoba oil, 20 parts of sweet almond oil, 55 parts of camellia seed oil, Mugwort complex essential oil is anti-inflammatory The effect is the strongest.

Test case 3

A total of 60 volunteers with sore throat were selected, 30 males and 30 females, aged 18-50 years old. The patients all had symptoms of sore throat, and there was no significant difference in the course and condition of the disease. They were randomly divided into 5 groups with 12 people in each group, including 6 males and females. Among them, the experimental group correspondingly applied the wormwood complex essential oil products of Examples 1 to 6 evenly on the sore throat, once in the morning, at night and once in the evening, with an amount of about 0.2ml each time; the control group did not use any products, and the patient’s throat was observed after 48 hours Pain conditions.

Efficacy criteria:

Significant: The throat pain is significantly reduced or basically disappeared, and the secondary symptoms are effectively relieved.

General: The sore throat is slightly weakened, and the secondary symptoms are partially improved.

Not obvious: Sore throat, no significant improvement in secondary symptoms.

Table 4 shows the test results of the Mugwort leaf compound essential oils in Examples 1 to 6 for relieving sore throat.

Table 4 Tests of the effects of the wormwood compound essential oils in Examples 1 to 6 in relieving throat pain

The results in Table 4 show that after applying the Mugwort complex essential oil products of Examples 1 to 6, the effect of alleviating throat pain is obvious compared with the control group, and the symptoms of more than 85% of patients have been improved to a certain extent; about 70% of patients have throat pain and secondary symptoms It is effectively relieved, which is more effective than the control. It can be seen that the wormwood compound essential oil of the present invention can effectively relieve the symptoms of sore throat, and its effect is remarkable. Moreover, after applying the wormwood compound essential oils of Examples 1 to 6, none of the subjects had any allergies, nor did they have skin itching, tingling, etc.; among them, the weight of the five essential oils was controlled to the wormwood essential oil 4 parts, 0.4 parts of lemon essential oil, 0.4 parts of tea tree essential oil, 0.2 parts of lavender essential oil, 1.5 parts of rosemary essential oil. The five essential oils can work together to relieve sore throat to the greatest extent.

The above embodiments are only preferred embodiments of the present invention, and only provide a further detailed description of the technical solution of the present invention to help understand the principle of the technical solution of the present invention. The various technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, this specification does not describe all possible combinations of the various technical features of the above-mentioned embodiments. However, as long as there is no contradiction in the combination of these technical features, it should be regarded as the scope described in this specification.

The above-mentioned embodiments only express several implementation modes of the present invention, and their description is relatively specific and detailed, but they should not be understood as a limitation on the scope of the invention patent. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made, and any changes and modifications made without departing from the principle and spirit of the present invention Combinations, deletions, replacements or modifications will all be included in the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention should be subject to the appended claims.

Claims (10)

1. A complex essential oil of mugwort leaf for relieving sore throat, which is characterized in that, in parts by mass, it comprises the following components:

   0.1 to 5.0 parts of red wormwood essential oil, 0.1 to 3.0 parts of lemon essential oil, 0.1 to 2.0 parts of tea tree essential oil, 0.1 to 2.0 parts of lavender essential oil, 0.1 to 6.0 parts of rosemary essential oil, 15.0 to 25.0 parts of jojoba oil, sweet almonds 15.0-25.0 parts of oil and 30.0-60.0 parts of camellia seed oil.
2. The wormwood compound essential oil for relieving sore throat according to claim 1, characterized in that, in parts by mass, it comprises the following components:

   Red foot wormwood essential oil 0.1～4.0 parts, lemon essential oil 0.4～1.6 parts, tea tree essential oil 0.2～0.5 parts, lavender essential oil 0.2～1.0 parts, rosemary essential oil 1.5～6.0 parts, jojoba oil 20.0～25.0 parts, sweet almond 20.0-25.0 parts of oil and 42.0-57.0 parts of camellia seed oil.
3. The wormwood compound essential oil for relieving sore throat according to claim 1, characterized in that, in parts by mass, it comprises the following components:

   Red foot wormwood essential oil 2.0 parts, lemon essential oil 0.8 parts, tea tree essential oil 0.2 parts, lavender essential oil 0.5 parts, rosemary essential oil 1.5 parts, jojoba oil 20.0 parts, sweet almond oil 20.0 parts, camellia seed oil 55.0 parts.
4. The wormwood compound essential oil for relieving sore throat according to claim 1 or 2, characterized in that the content of the red wormwood essential oil is 0.1~ 5.5%.
5. The wormwood compound essential oil for relieving sore throat according to any one of claims 1 to 3, wherein the wormwood essential oil is obtained by the following method:

   The dried wormwood leaves are taken, crushed, and _{extracted with supercritical CO 2 to} separate the wormwood leaves extract; then molecularly distilled to obtain the crude oil of wormwood leaves, stand still, and filter to obtain the wormwood essential oil.
6. The wormwood compound essential oil for relieving throat pain according to claim 5, wherein the supercritical CO ₂ extraction temperature is 30-60°C, the extraction pressure is 10-15 MPa, and the extraction time is 1 to 3 hours.
7. The Mugwort leaf compound essential oil for relieving throat pain according to claim 5, wherein the separation temperature is 30-60°C, and the separation pressure is 4-7 MPa.
8. The wormwood compound essential oil for relieving sore throat according to claim 5, characterized in that the temperature of the molecular distillation is 90-120°C, the vacuum degree is 50-200 Pa, the internal condensation temperature is 50-70°C, and the temperature is cold. The temperature of the trap is -15～0℃.
9. The wormwood compound essential oil for relieving sore throat according to any one of claims 1 to 3, wherein the lemon essential oil, tea tree essential oil, lavender essential oil and rosemary essential oil are obtained by the following methods:

   Put the fresh lemon peel, Australian tea leaves, lavender, and rosemary in the volatile oil extractor respectively, and extract by steam distillation for 2 to 4 hours, collect the primary essential oils, and then fumigate the primary essential oils by water distillation for 2 to 4 hours. Obtain all finished essential oils.
10. The method for preparing a wormwood compound essential oil for relieving sore throat according to any one of claims 1-9, characterized in that it comprises the following steps:

    According to the mass parts, mix the red wormwood essential oil, lemon essential oil, lavender essential oil, tea tree essential oil, rosemary essential oil, jojoba oil, sweet almond oil and camellia oil evenly to obtain the sore throat relief Mugwort complex essential oil.

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