KR101224343B1 - The manufacturing method of firs's leaf oil refning which relaxes the skin pruritis - Google Patents

Abstract

The present invention relates to a method for producing a fir leaf essential oil to alleviate skin pruritus, wherein the fir leaf is washed, and then the fir leaf pretreatment step (first step); An alcohol mixing step (second step) of mixing alcohol at a ratio of 1: 1 by weight to the fir leaves pretreated in the first step; Steam collecting step (third step) to collect the vapor containing the essential oil by heating the fir leaf solution mixed with alcohol in the second step at 90 ~ 110 ℃ 1 hour; And the essential oil collected in the third step It is characterized by consisting of a cooling step (fourth step) to obtain a steamed leaf oil by cooling the steam containing 1 to 2 hours at 0 ~ 20 ℃.
Fir leaf essential oil according to the present invention is alpha pinene (α-Pinen), camphor (Camphen), beta pinene (β-Pinen), myrene (Myrcene), Karen (Caren), Sabinene (Libinen), Limonen (Limonen) , Terpinene, Borneol, Caryophyllene, etc., are used to relieve skin irritation caused by itching, swelling, wounds, boils, swelling, etc. In addition, it is excellent for skin troubles such as skin dryness, rash, psoriasis, vitiligo, and skin metabolism promotion, and the fragrance of fir leaf essential oil relieves fatigue, headache, severe cough, asthma and neuralgia, depression, insomnia Psychological stabilizing effect on back stress neurological disease is great.

Description

The manufacturing method of fir leaf essential oil which alleviates skin pruritus {THE MANUFACTURING METHOD OF FIRS'S LEAF OIL REFNING WHICH RELAXES THE SKIN PRURITIS}

The present invention relates to a method for producing a fir leaf essential oil to alleviate pruritus by using on skin with pruritus, wherein the fir leaf is washed, and then the fir leaf pretreatment step (first step); An alcohol mixing step (second step) of mixing alcohol at a ratio of 1: 1 by weight to the fir leaves pretreated in the first step; Steam collecting step (third step) to collect the vapor containing the essential oil by heating the fir leaf solution mixed with alcohol in the second step at 90 ~ 110 ℃ 1 hour; And the essential oil collected in the third step It is made of a cooling step (fourth step) to obtain a fir leaf essential oil by cooling the steam containing 1 to 2 hours at 0 ~ 20 ℃.

Fir is an evergreen tree of the pine tree family of creeper trees, also called chopped tree. Alpine plant 40m high and 1.5m in diameter. The bark is grayish brown, coarse, small branches grayish brown, hairless or sometimes with shallow grooves. Winter snow is egg-shaped, hairless but slightly resinous. The leaves are spirally arranged in a row, 4cm in length, 2mm in butterfly, pointed at the end, with white pores on the back, and resin spheres in the cross section. Flowers are bisexual and bloom in late April. Male spikes are cylindrical, 15mm long, yellowish green, and flower stem is 4.5mm long. The female flower spikes are 2 to 3 running close to each other, 3.5cm long, long oval, and 6mm in length.

Fir leaf essential oils include alpha pinene (α-Pinen), camphor (Camphen), beta pinene (β-Pinen), myrcene, Karen, Sabinene, limonen and terpin ( Terpinene), Borneol (Borneol), Caryphyllene (Caryophyllene) and the like, the fir leaf essential oil has a good effect on various inflammatory diseases such as rheumatoid arthritis, back pain, urethritis, pulmonary tuberculosis, gastritis, gastric ulcer, itching When used in skin with skin pruritus, such as swelling, wounds, boils, swelling, etc., it is not only relieved, but also excellent for skin problems such as dry skin, rashes, psoriasis, vitiligo, and skin metabolism.

Skin pruritus is basically a condition in which itching is the disease itself, and itching is perceived only in the skin, mucosa and cornea. This perception is limited to skin skin connections. Itching is a common sensation in most skin diseases with pain, and it is defined as an unpleasant sensation that causes the urge to scratch in clinical practice (Yosipovitch et al, The Lancet, 361, pp, 690-93, 2003). The causes of localized or systemic pruritus vary. Extensive itching or generalized pruritis is caused by many external factors and internal conditions. The most common causative agents are environmental triggers, including climate, particulate matter, detergents, skin infections of fungi or bacteria, insects and invasion of the body. A second common factor is caused by biliary atresia or kidney failure (Sonja et al, Arch. Dermatol., 139, pp. 1443-70, 2003). Specifically, skin diseases in which itching is a major problem include hives, dry skin, seborrheic dermatitis, psoriasis and insect bites (Greaves et al, The Lancet., 348, pp. 938-40, 1996). In addition, the skin pruritus includes itching caused by inflammatory dermatitis, dermatitis due to skin roughness, contact dermatitis due to sweat bands, sores, frostbite, diaper, psoriasis or psoriasis. As shown in the above classification of causes and symptoms, itching is the primary cause of bacterial or fungal infection of the body, or influenza poison or environmental toxicity, and secondly, the immune system against these primary causes. It refers to a series of phenomena leading to the working phenomena of inflammation, swelling, and perception of itching. Therefore, antibiotics, antimicrobials, or anti-toxics or immunosuppressive methods are expected to be therapies. In practice, itching corticosteroids, antihistamines, immunosuppressants, capsaicin, etc. are used, but these treatments have their own side effects. Topical steroids can cause skin thinning, skin color changes, rashes, and long-term use of large amounts of systemic side effects that can inhibit adrenal function. First-generation antihistamines are mainly used systemically, and have anti-sympathetic effects, and chlorpheniramine, a first-generation antihistamine, did not suppress the itching of patients with atopic dermatitis when administered topically (Munday et al., Dermatology, 205, pp 40-45, 2002). In addition, the use of topical antihistamines for atopic dermatitis is not recommended because there is a risk of skin hypersensitivity. The second generation of antihistamines, eastin and terpenadine, which are non-sedative, can cause arrhythmia when combined with drugs that inhibit cytochrome P450 activity (ketoconazole, erythromycin) (Hey et al., Arnezimittelforschung, 46, pp159 -163, 1996). Cyclosporine, an immunosuppressive agent, can cause serious side effects such as hypertension, nephrotoxicity, and drug interactions during systemic administration, and its high molecular weight makes it difficult to reach the skin through organs. Calcineurin inhibitors, recently developed as topical agents, protopic (tacrolimus, FK506) and ellidel (pimecrolimus) have been shown to increase the incidence of leukemia in pediatric patients, furthermore redness, irritation, and itching early in use. , Erythema and other side effects have been reported (Gupta et al., JEADV, 16, pp. 100-114, 2002; Gupta and Chow, JEADV, 17, pp. 493-503, 2003). Capsaicin is widely known to be the fastest and most potent anti-itch, but has limited use due to strong skin irritation. As seen above, subcutaneous intravenous injections or doses should be premised on the condition that they should be able to reach the cell with a small enough molecular weight to have an effect. In addition, both systemic and topical preparations should have no side effects, and agents that inhibit the operation of the immune system or suppress the parasympathetic nerve should be applied only temporarily to not disturb the immune system itself. Therefore, there is an urgent need for effective, side effects and safe topical itch relievers.

The present invention is invented to solve the above problems, and after firing the fir leaves, fir leaf pre-treatment step (first step); An alcohol mixing step (second step) of mixing alcohol at a ratio of 1: 1 by weight to the fir leaves pretreated in the first step; Steam collecting step (third step) to collect the vapor containing the essential oil by heating the fir leaf solution mixed with alcohol in the second step at 90 ~ 110 ℃ 1 hour; And the essential oil collected in the third step It is an object of the present invention to provide a method for preparing a fir leaf essential oil to alleviate skin pruritus consisting of a cooling step (fourth step) of cooling steam containing 0 to 2 hours at 0 to 20 ° C. to obtain a fir leaf essential oil.

Fir leaf essential oils prepared by the above method are alpha pinene (α-Pinen), camphor (Camphen), beta pinene (β-Pinen), myrcene, Karen (Caren), Sabinene (Sabinene), limonene ( Limonen, Terpinene, Borneol, Caryophyllene, etc., are used to relieve skin irritation caused by itching, swelling, wounds, boils, and swelling. Not only effective, but also excellent for skin troubles such as dry skin, rash, psoriasis, vitiligo, and skin metabolism, and the fragrance of fir leaf essential oil relieves fatigue, headache, severe cough, asthma and neuralgia, depression It has a great psychological stability effect on stressful neurological diseases such as insomnia and insomnia.

In order to achieve the above object, the method for preparing a fir leaf essential oil to alleviate skin pruritus according to the present invention comprises: fir leaf pre-treatment step (first step), after washing the fir leaf; An alcohol mixing step (second step) of mixing alcohol at a ratio of 1: 1 by weight to the fir leaves pretreated in the first step; Steam collecting step (third step) to collect the vapor containing the essential oil by heating the fir leaf solution mixed with alcohol in the second step at 90 ~ 110 ℃ 1 hour; And the essential oil collected in the third step It is characterized by consisting of a cooling step (fourth step) to obtain a steamed leaf oil by cooling the steam containing 1 to 2 hours at 0 ~ 20 ℃.

In addition, after the cooling step of the fourth step, it is characterized in that it further comprises a herb essential oil mixing step (step 5) of mixing the obtained fir leaf essential oil and herb essential oil in a ratio of 1: 1 by weight.

In addition, the herbal essential oil of the fifth step is prepared by mixing one or two or more of jasmine, peppermint, rosemary, fennel, cinnamon, chamomile, lemongrass, lemon balm, rosehip, sage, thyme, basil, basil. It is done.

Fir leaf essential oil to alleviate the skin pruritus according to the present invention is alpha pinene (α-Pinen), camphor (Camphen), beta pinene (β-Pinen), myrcene, Karen (Caren), Sabinene (Sabinene) , Limonen, Terpinene, Borneol, Caryophyllene, etc.It is used for skin with pruritus caused by itching, swelling, wounds, boils, swelling, etc. It is not only effective in relieving skin, but also excellent in skin troubles such as dry skin, rash, psoriasis, vitiligo, and skin metabolism. The fragrance of fir leaf essential oil relieves fatigue, headache, severe cough, and asthma. Psychological stable effects on stress neurological disorders such as neuralgia, depression and insomnia.

1 is a flow chart schematically showing a method for producing a fir leaf essential oil to alleviate skin pruritus according to the present invention.

Hereinafter, the manufacturing method of the present invention will be described in detail with reference to the accompanying drawings.

1 is a flow chart schematically showing a method for producing a fir leaf essential oil to alleviate skin pruritus according to the present invention.

1. Fir leaf pretreatment step (1st step)

Fir leaf pretreatment step is to wash and crush the fir leaf,

The fir leaves are washed in water and crushed to a size of 0.5 to 10 mm.

In addition, the fir leaves are alpha pinene (α-Pinen), camphor (Camphen), beta pinene (β-Pinen), myrene (Myrcene), Karen (Caren), sabiene (Sabinene), limonene (Limonen), ter It contains Terpinene, Borneol and Caryophyllene, and fir leaf essential oil is used for various inflammatory diseases such as rheumatoid arthritis, back pain, urethritis, pneumonia, pulmonary tuberculosis, gastritis, gastric ulcer and duodenal ulcer. It has a good effect, and drinking the fragrance from fir leaf essential oils has the effect of relieving fatigue, headache, severe cough, asthma, and psychological stabilizing effect on stress neurological diseases such as neuralgia, depression, insomnia, and itching and swelling when applied to the skin. When used in the skin with skin pruritus, such as wounds, boils, swelling, swelling, etc., not only has a relieving effect, but also excellent for skin problems such as dry skin, rashes, psoriasis, vitiligo, and skin metabolism.

In addition to the leaves of the fir, roots, stems can be used.

Here, the fir leaves are mainly used because not only the essential oil is contained in the fir leaves, but also when the roots and stems of the firs have a problem in that the whole firs are damaged.

And, washing the fir leaves in water is to remove impurities of the fir leaves.

And, after washing the fir leaf, the crushed to 0.5 ~ 10mm size is to allow the fir leaf to easily penetrate the alcohol in the alcohol mixing step below.

If, after washing the fir leaf, the crushed to a size of less than 0.5mm, because the alcohol is easily penetrated into the fir leaf is more pulverized than necessary because it is uneconomical and the particles are small, the scattering is worse It may cause inconvenience to the production, and when pulverized to a size exceeding 10mm, alcohol may be difficult to penetrate easily into the fir leaves.

2. Alcohol mixing step (second step)

Alcohol mixing step is to mix the alcohol to the pre-treated fir leaf in the first step,

In the first step is to mix the alcohol in a ratio of 1: 1 by weight of the fir leaves pretreated.

The mixing of alcohol in the ratio of 1: 1 to the weight of the fir leaves pretreated in the first step is to allow the essential oil contained in the fir leaves pretreated in the first step to be sufficiently eluted.

If the alcohol is mixed in a ratio of less than 1: 1 to the weight of the fir leaves pretreated in the first step, the essential oil contained in the fir leaves pretreated in the first step may not be sufficiently eluted. When the alcohol is mixed in the ratio of 1: 1 to the weight of the fir leaves pretreated in the first step, more alcohol is required to elute the essential oil contained in the fir leaves pretreated in the first step. It is uneconomical and the heating time takes longer in the steam collection step below.

In this case, the alcohol is preferably used ethanol.

Here, the use of the ethanol is not only to increase the safety when the ethanol is used for extraction, it is also suitable for use as an additive to food and cosmetics because it is harmless to humans, and the cost increase such as strict process control to ensure safety Because it does not occur, it is economical.

In addition, a mixture of alcohol in a ratio of 1: 1 by weight to the fir leaf pretreated in the first step is called fir leaf solution.

3. Steam collection stage (3rd stage)

Steam collecting step is to collect the steam containing essential oil by heating the fir leaf solution mixed with alcohol in the second step,

In the second step, the fir-leaf solution mixed with alcohol is heated at 90 to 110 ° C. for 1 to 2 hours to collect steam containing essential oils.

Here, when collecting the steam containing the essential oil by heating the fir leaf solution mixed with alcohol in the second step at 90 ~ 110 ℃ for 1 hour, steam collection method is generally when extracting essential oil in the art The detailed description thereof will be omitted.

In addition, heating the fir leaf solution mixed with alcohol in the second step at 90 to 110 ° C. for 1 to 2 hours may cause vaporization of the alcohol in the fir leaf solution mixed with alcohol in the second step and vapor containing essential oil. To get it.

If the fir leaf solution in which alcohol is mixed in the second step is heated to 90 ° C. for less than 1 hour, the alcohol does not sufficiently evaporate in the fir leaf solution in which the alcohol is mixed in the second step. It may be difficult to obtain easily, if the alcohol is mixed in the fir leaf solution mixed with alcohol in the second step 110 ℃, heating for more than 2 hours, the alcohol is evaporated from the fir leaf solution mixed with alcohol in the second step and refined It is uneconomical to consume more heating temperature and heating time than is necessary to obtain steam containing.

4. Cooling stage (fourth stage)

The cooling step is to cool the steam containing the essential oil collected in the third step,

The steam containing the essential oil collected in the third step is cooled at 0 to 20 ° C. for 1 to 2 hours to obtain fir leaf essential oil.

And, what is obtained by cooling the steam containing the essential oil collected in the third step at 0 ~ 20 ℃ 1-2 hours is called fir leaf essential oil.

 Here, when the steam containing the essential oil collected in the third step is cooled for 1-2 hours at 0 ~ 20 ℃ to obtain the fir leaf essential oil, the cooling method is generally used when extracting essential oil in the art The detailed description thereof is omitted.

The cooling of the steam containing the essential oil collected in the third step at 0 to 20 ° C. for 1 to 2 hours is to sufficiently obtain the fir leaf essential oil from the steam containing the essential oil collected in the third step.

If the steam containing the essential oil collected in the third step is cooled at a temperature below 0 ° C., it is difficult to easily obtain the fir leaf essential oil from the steam containing the essential oil collected in the third step due to the low temperature. When the steam containing the essential oil collected in the third step is cooled at a temperature exceeding 20 ℃, steam containing essential oil collected in the third step is not cooled smoothly fir leaf essential oil Can be difficult to get.

In addition, if the water vapor containing the essential oil collected in the third step is cooled to less than 1 hour at 0 ~ 20 ℃, the cooling is not sufficiently made fir leaf in the water vapor containing the essential oil collected in the third step If the essential oil cannot be sufficiently obtained, and the steam containing the essential oil collected in the third step is cooled for more than 2 hours at 0-20 ° C., the fir leaf essential oil is removed from the steam containing the essential oil collected in the third step. It is uneconomical due to the cooling time longer than necessary to obtain.

Fir leaf essential oils prepared by the above method are alpha pinene (α-Pinen), camphor (Camphen), beta pinene (β-Pinen), myrene (Myrcene), Karen (Saenene), limonene ( Limonen, Terpinene, Borneol, Caryophyllene, etc., are used to relieve skin irritation caused by itching, swelling, wounds, boils, and swelling. Not only effective, but also excellent for skin troubles such as dry skin, rash, psoriasis, vitiligo, and skin metabolism, and the fragrance of fir leaf essential oil relieves fatigue, headache, severe cough, asthma and neuralgia, depression It has a great psychological stability effect on stressful neurological diseases such as insomnia and insomnia.

In addition, after the cooling step of the fourth step, it may further include a herb essential oil mixing step (step 5) of mixing the fir leaf essential oil and the herbal essential oil obtained in the fourth step.

5. Herb essential oil mixing step (Step 5)

Herb essential oil mixing step is a step of mixing the fir leaf essential oil and herb essential oil obtained in the fourth step,

The fir leaf essential oil and the herbal essential oil obtained in the fourth step is to be mixed in a ratio of 1: 1 by weight.

In addition, the herbal essential oil is to extract the essential oil from the herb, the extraction method is a method commonly used in the art, the specific method is omitted.

In addition, when mixing the fir leaf essential oil and the herbal essential oil obtained in the fourth step is a method generally used in the art is not limited to the method, but is preferably uniformly mixed using a stirrer.

In addition, the herbal essential oils are generally used in the art, and are not limited in kind.

Preferably, jasmine, peppermint, rosemary, fennel, cinnamon, chamomile, lemongrass, lemon balm, rosehip, sage, thyme, high boss, characterized in that prepared by mixing one or two or more of basil.

In addition, the herbal essential oils have excellent effects on neurological diseases such as skin elasticity, skin disease improvement, stress, as well as the inherent flavor of herbs.

More specifically, the jasmine gives elasticity to dry sensitive skin and is effective in normalizing menstruation, relieving postpartum pain, promoting breast milk, coldness, stressful stomach pain, depression, and the like. The peppermint cools the skin and mucous membranes and relieves antibacterial and pain. It is effective in rejuvenation, bath additives, fatigue and depression, nervous attacks, fever and cold protection, and is effective in colds, asthma, bronchitis, cholera, pneumonia, pulmonary tuberculosis, food poisoning, neuralgia, etc. The rosemary prevents aging, headache relief, digestion Efficacy in promoting, enhancing memory, etc. The pennel is purifying, tonic, expectorant, healthy, insecticidal, antiquatic, antiperspirant, insecticidal, disinfecting, anti-inflammatory, edible promoting, alleviating, diuretic Efficacy in detoxification, etc. The cinnamon has anti-inflammatory, bactericidal, detoxification and vitality and vitality effects on the skin. The chamomile is effective in skin soothing effect, anti-inflammatory effect, allergy, bacteria, fungal skin inflammation, and the lemongrass is effective in skin elasticity, skin impurity removal, stress relief, nasal cold, anemia, etc. Lemon balm is effective in treating wound inflammation, preventing aging, promoting memory, improving the cardiovascular system, depression, neuropathic pain, digestive respiratory disease, memory loss, toothache, and the like. It has an excellent effect on skin aging, and the sage has the effect of disinfection and antiseptic on antiseptic, antibacterial, etc., and it is effective in antipyretic, old wind, blood circulation and fatigue recovery. It is effective in preventing colds, coughing, skin cleansing, fatigue, and relieving stress. The high boss is effective in dermatitis, diabetes, and allergic diseases. And the basil has the effect of acute inflammatory skin diseases such as healing, it gives elasticity and vitality to the skin, digestive problems, nerve disorders.

Here, mixing the fir leaf essential oil and the herbal essential oil obtained in the fourth step in a ratio of 1 to 1 by weight, the herbal essential oil is mixed with the fir leaf essential oil obtained in the fourth step to relieve skin pruritus, as well as more fragrance In addition, the optimal mixing ratio was derived to further maximize the efficacy of the improvement of the neurological diseases such as skin recovery and stress such as skin elasticity, whitening, anti-aging, and the improvement of digestive diseases.

If the fir leaf essential oil and the herbal essential oil obtained in the fourth step is mixed at a ratio of less than 1: 1 by weight, the fragrance of the fir leaf essential oil may not be strong, the fragrance of the herbal essential oil, the efficacy of the herbal essential oil It may not be sufficiently exhibited, and when the fir leaf essential oil and the herbal essential oil obtained in the fourth step are mixed in a ratio of more than 1: 1 by weight, the fragrance of the fir leaf essential oil may not be sufficient, Efficacy in relieving skin pruritus may not be exerted enough.

Fir leaf essential oil prepared by the above method for alleviating skin pruritus is not particularly limited in its use, but is used by applying or spraying on the skin for alleviating skin pruritus and drinking the fragrance emitted from the fir leaf essential oil. It is preferable.

More specifically, the formulation is selected from sprays, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, cleansing, foundations, and perfumes.

Hereinafter, the present invention will be described more specifically by way of examples. It will be apparent to those skilled in the art, however, that the following examples are illustrative of the present invention only and do not limit the scope of the present invention. That is, a simple modification or change of the present invention can be easily performed by those skilled in the art, and all such modifications and alterations can be considered to be included in the scope of the present invention.

Example 1 Preparation of Essential Oil of Fir Leaf Prepared According to the Present Invention

After washing 1 kg of fir leaves and pulverizing to 5 mm, the alcohol was mixed at a ratio of 1 to the weight of the fir leaves, and heated at 100 ° C. for 1 hour and 30 minutes to collect water vapor, and the collected water vapor at 1 ° C. at 1 ° C. Prepared by cooling for 30 minutes.

The prepared fir leaf essential oil was 42.50 g.

Example 2 Preparation of Fir Leaf Essential Oils Prepared by Mixing Herbal Essential Oils According to the Present Invention

After washing 1 kg of fir leaves and pulverizing to 5 mm, the alcohol was mixed at a ratio of 1 to the weight of the fir leaves, and heated at 100 ° C. for 1 hour and 30 minutes to collect water vapor, and the collected water vapor at 1 ° C. at 1 ° C. Prepared by cooling for 30 minutes.

Then, 40 g of the fir leaf essential oil prepared above and 40 g of herb essential oil are mixed.

Comparative Example 1 prepared by mixing alcohol at a ratio of 0.5 times the weight of fir leaf

After washing 1 kg of fir leaves and pulverizing to 5 mm, the alcohol was mixed at a ratio of 0.5 times to the weight of the fir leaves, and heated at 100 ° C. for 1 hour and 30 minutes to collect water vapor, and the collected water vapor at 1 ° C. at 1 ° C. Prepared by cooling for 30 minutes.

The fir leaf essential oil thus prepared was 27.80 g.

Comparative Example 2: Prepared by mixing alcohol at a ratio of 2 times the weight of fir leaf

After washing 1 kg of fir leaves and pulverizing to 5 mm, the alcohol was mixed at a ratio of 2 times to the weight of the fir leaves, and heated at 100 ° C. for 1 hour and 30 minutes to collect water vapor, and the collected water vapor at 1 ° C. at 1 ° C. Prepared by cooling for 30 minutes.

The manufactured fir leaf essential oil was 31.50 g.

Comparative Example 3: Prepared by mixing the herbal essential oil at a ratio of 0.5 times to the weight of the fir leaf essential oil

After washing 1 kg of fir leaves and pulverizing to 5 mm, the alcohol was mixed at a ratio of 1 to the weight of the fir leaves, and heated at 100 ° C. for 1 hour and 30 minutes to collect water vapor, and the collected water vapor at 1 ° C. at 1 ° C. Prepared by cooling for 30 minutes.

Then, 40 g of the fir leaf essential oil prepared above and 20 g of herb essential oil are mixed.

Comparative Example 4: Prepared by mixing the herbal essential oil at a ratio of 2 times the weight of the fir leaf essential oil

After washing 1 kg of fir leaves and pulverizing to 5 mm, the alcohol was mixed at a ratio of 1 to the weight of the fir leaves, and heated at 100 ° C. for 1 hour and 30 minutes to collect water vapor, and the collected water vapor at 1 ° C. at 1 ° C. Prepared by cooling for 30 minutes.

Then, 40 g of the fir leaf essential oil prepared above and 80 g of herb essential oil are mixed.

Experiment 1: Analysis of Essential Oil Components of Fir Leaf Prepared According to the Present Invention

In order to determine the components contained in the essential oil of the fir leaf prepared according to the present invention was commissioned by the Korea Institute of Chemical Research and analyzed by the name of the sample "pine forest".

The analysis was performed by Agilent 689ON Gas Chromatograph / 5973N Mass Selective Detector (GC / MSD), and the GC / MSD analysis conditions are shown in Table 1 below, and the analysis results are shown in Table 2.

Analysis condition Column DB-1HT (30m × 0.25mm × 0.1㎛) Imjector Temp 250 ℃ Oven temp 30 ℃ (3min) to 300 ℃, rate: 10 ℃ / min Ionization mode EI, 70 eV

ingredient content(%) Alpha pinene (α-Pinen) 7.719 Campen 9.793 Beta pinene (β-Pinen) 1.625 Myrcene 7.563 Karen 17.561  Sabinene 2.367 Limonen 2.912 Terpinene 1.306 Borneol 6.657 Heptanol  41.144 Caryophyllene 1.352

Experiment 2: Experimental Extraction Ratio of Fir Leaf Essential Oil According to Alcohol Mixing Ratio

In Example 1, Comparative Example 1, Comparative Example 2, an experiment was conducted on the essential oil content extracted from the fir leaves according to the alcohol mixing ratio. The results are shown in Table 3 below.

Fir leaf essential oil content (%) Example 1 4.25 Comparative Example 1 2.78 Comparative Example 2 3.15

As shown in Table 3, Example 1 of the fir leaf and alcohol mixing ratio according to the present invention in a ratio of 1 to 1 was the content of the fir leaf essential oil was 4.25%, fir leaf and alcohol mixing ratio by weight In Comparative Example 1, the content of fir leaf essential oil was 2.78% in Comparative Example 1, which was prepared at a ratio of 1: 0.5. It was%.

Therefore, it can be seen that the fir leaf essential oil content of Example 1 prepared according to the present invention is the highest. In other words, it can be said that the fir leaf essential oil is extracted the most when the fir leaf and alcohol in a ratio of 1: 1 by weight.

As in Comparative Example 1, when the fir leaf and alcohol were prepared in a ratio of 1: 0.5 by weight, the essential oil contained in the fir leaf was not sufficiently eluted, so that the content of the fir leaf essential oil was low, and as in Comparative Example 2, the fir leaf When alcohol and alcohol is produced in a ratio of more than 1: 1 by weight, alcohol more than necessary to elute the essential oil contained in the fir leaf is used, rather it causes inconvenience in manufacturing, and thus the fir leaf oil cannot be easily eluted. It was.

Experiment 3: Sensory Test

Fir leaf essential oil (Example 1) prepared according to the present invention, and the essential oil (Example 2) and fir leaf essential oil and herb essential oil blended in a ratio of 1: 1 by weight of fir leaf essential oil and herb essential oil 1: The essential oils (comparative example 3) mixed with the ratio of 0.5 and the essential oils (comparative example 4) mixed with the essential oils of the fir leaf and the herb essential oils at the ratio of 1: 2 by weight are sensory test agents (30 years with sensory test experience) 15 people (15 males, 15 females) divided into incense, color, and overall taste sensory test (5 points measuring method: 1: very bad, 2: bad, 3: normal, 4: good, 5: very good) Was carried out. The results are shown in Table 4.

incense Color Overall preference Example 1 5 4 5 Example 2 5 5 5 Comparative Example 3 3 4 4 Comparative Example 4 3 3 3

As can be seen from Table 4, Examples 1 and 2 show higher scores than Comparative Examples 3 and 4 in all items.

The reason why Examples 1 and 2 were higher than Comparative Examples 3 and 4 in all the items was that the fragrance and color of fir leaf essential oil gave a psychologically stable effect on fatigue recovery and stress neurological disease as a whole. In addition, it can be seen that the herb essential oil is properly mixed with the fir leaf essential oil to show a preference in flavor or color.

Experiment 4: Skin Stimulation Experiment

Stability test for skin irritation of the composition containing the essential oil of fir leaf to relieve skin pruritus prepared according to the present invention (Example 1) was carried out based on the toxicity test criteria such as pharmaceuticals.

Here, 24 rabbits (7 rabbits), 7 weeks old Juryland White, were confirmed to have no skin infection by bacteria and fungi after 7 days of purifying period purchased from Hyochang Corporation for the above skin irritation experiment. Before the time, the back of the rabbit was epilated using an electric clipper, and each rabbit was divided into a rubbing and a rubbing spot, and the experiment was performed. Damage to the epidermis does not bleed to create a scratch condition.

After fixing 3 layers of 3cm * 3cm gauze to each rabbit that distinguishes between the rubbing and skin parts, the 100 parts of rabbits (rabbit 1, rabbit 2, rabbit 3) with gauze fixed 100 times with distilled water. The diluted Example 1 was absorbed by 5 ml (hereinafter referred to as 'experimental sphere'), and the remaining three animals (rabbit 4, rabbit 5, rabbit 6) were absorbed by 5 ml of distilled water (hereinafter referred to as 'control').

In addition, the experimental group and the control group were observed the clinical symptoms every 24 hours, 48 hours, 72 hours while providing the same feed and negative for 3 days, and evaluated the skin response due to stimulation.

Here, the evaluation of the skin reaction is based on the toxicity test standards (National Institute of Safety, 1994), such as drugs of the National Institute of Health and Safety No. 94-3, stimulation for the primary skin irritation response Grade; according to Draize (1959), Obra (1992)), erythema, crust and edema were observed in experimental and control groups and skin reactions were evaluated.

In addition, the clinical symptoms of the experimental and control groups were observed, and the evaluation of the skin response due to the stimulation is shown in Table 6 below.

Rating Range of Primary Stimulus Index Mild irritant <2 Moderate irritant 2 to 5 Severe irritant > 5

Mirror and site Abrasions 24 (hr) 48 (hr) 72 (hr) 24 (hr) 48 (hr) 72 (hr) Experimental Section Rabbit 1 0 0 0 0 0 0 Rabbit 2 0 0 0 0 0 0 Rabbit 3 0 0 0 0 0 0 Control Rabbit 4 0 0 0 0 0 0 Rabbit 5 0 0 0 0 0 0 Rabbit 6 0 0 0 0 0 0

As can be seen from Table 6, the skin, such as erythema and skin phenomenon, edema phenomenon in the gauze fixed in the experimental gauze absorbed in Example 1 diluted 100-fold with distilled water, such as the control gauze fixed in distilled water absorbed As the reaction does not appear it can be confirmed that there is no irritation on the skin.

Experiment 5: Confirmation of skin pruritus relief effect

Composition containing fir leaf essential oil prepared in accordance with the present invention divided into 10 each for a total of 20 people who are scratched at least once per minute to recognize severe itching within 30 minutes after at least two mosquito bites Example 1 using a spray containing the experimental (test), treated with nothing control for 1 hour, before treatment with the experimental and control, itching for 1 minute, 10 minutes, 1 hour after treatment Table 7 shows the number of people who felt.

Here, the definition of itching was defined as the state in which the hand went more than twice in the itchy area.

Before processing 1 minute later After 10 minutes After 1 hour Experiment
(10 people) 10 7 5 2 Control
(10 people) 10 10 9 8

As can be seen in Table 7, the mosquito bite at least two places within 30 minutes after perception of severe itching, compared to the control group did not use anything to the person who is scratched at least once a minute, prepared according to the present invention It can be seen that the experimental group treated with the composition containing the fir leaf essential oils feel less itching.

Thus, it can be seen that the fir leaf essential oil prepared according to the present invention alleviates skin pruritus.

Claims (4)

After washing the fir leaves, fir leaf pre-treatment step (first step) for crushing the fir leaf to 0.5 ~ 10mm size;
An alcohol mixing step (second step) of mixing alcohol in a ratio of 1: 1 by weight to the fir leaves pretreated in the first step;
Steam collecting step (3rd step) to collect the steam containing the essential oil by heating the fir leaf solution mixed with alcohol in the second step at 90 ~ 110 ℃ 1 ~ 2 hours;
A cooling step (fourth step) of cooling the steam containing essential oil collected in the third step at 5 to 20 ° C. for 1 to 2 hours to obtain fir leaf essential oil;
Herbal essential oil prepared by mixing one or two or more of the fir leaf essential oil obtained in the fourth step with jasmine, peppermint, rosemary, fennel, cinnamon, chamomile, lemongrass, lemon balm, rosehip, sage, thyme, basil Method for producing a skin leaf relieving fir leaf essential oil, characterized in that consisting of a herbal essential oil mixing step of mixing in a ratio of 1: 1 by weight (the fifth step). delete delete delete

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