CN114259430B - Plant essential oil antibacterial spray and preparation method thereof - Google Patents
Plant essential oil antibacterial spray and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a plant essential oil antibacterial spray and a preparation method thereof, wherein the antibacterial spray comprises A, B, C three phases, and the weight of the antibacterial spray is 100 parts, wherein A: 0.01-5 parts of spearmint essential oil, 0.01-1 part of lavender essential oil, 0.01-1 part of atractylis lancea essential oil, 0.01-1 part of wormwood essential oil, 0.01-1 part of sweet wormwood essential oil and 0.05-10 parts of propylene glycol; b: 0.01-1 part of phenoxyethanol and 0.1-0.5 part of octoxyglycerol; c: sodium citrate 0.01-0.1, bronopol 0.01-0.05, tween 20.5-10, sodium hyaluronate 0.01-0.1, defoamer 0.01-0.3, and spearmint hydrolat for the rest. The invention can effectively improve the bacteriostasis rate of pseudomonas aeruginosa, staphylococcus aureus and candida albicans, has obvious moisturizing effect on skin, has simple and easy operation process, is bacteriostasis, moisturizing and skin protection, has no toxic or side effect, and is safe and reliable.
Description
Technical Field
The invention belongs to the field of daily chemicals, and in particular relates to a plant essential oil antibacterial spray and a preparation method thereof.
Background
With the enhancement of resident health and disinfection consciousness, medical protection and disinfection articles can be continuously increased for a long time in the future, especially in the new crown epidemic situation, the demand of the disinfection articles can be increased in various regions of the whole country, the market scale of the disinfection products can be continuously expanded, and especially the demand of disinfection and bacteriostasis spraying in daily life is more indispensable, so that the disinfection and bacteriostasis spraying is a necessary article for the families and the trips of the masses. The existing antibacterial spray products in the market are various in variety, however, various chemical synthetic substances are added in most of the products, and the substances are not easy to degrade and pollute the environment; in addition, the antibacterial spray has single general efficacy and only has the functions of disinfection and bacteriostasis. Therefore, development of a bacteriostatic spray product containing natural plant active ingredients and having multiple functions is a significant need in the disinfection field. The antibacterial spray can regulate the flora balance, inhibit the growth of bacteria, prevent overgrowth of bacteria and harm health.
The research and utilization of essential oil at the current stage are relatively less, and Chinese patent CN 108938795A skin antibacterial spray and a preparation process thereof mainly introduce the steps of mixing lavender essential oil, honeysuckle essential oil, medicinal material concentrated solution of cinnamon with large leaf fragrance and phellodendron bark and part of chemical cosolvent, wherein the preparation process of the medicinal material concentrated solution is relatively complicated; chinese patent CN 108164785A, "a plant essential oil type insect-killing and insect-expelling master batch, a preparation method and application thereof", introduces that finished essential oil such as sabina chinensis, hunter Mao Hao, yunnan incense, spearmint and the like is mixed with antioxidant, talcum powder and the like, and is mainly applied to the technical field of insect-killing and insect-expelling of agricultural mulching films and greenhouse films; chinese patent CN 111700848A, a plant antibacterial spray and a preparation method thereof, introduces a mixture and stirring of combination essential oil of eucalyptus, cinnamomum camphora, green cajeput, verbenone rosemary and the like, a plurality of plant compound extract solutions, aromatic agents, cosolvents and the like, and has numerous varieties and redundant procedures.
According to the invention, five natural plant essential oils of spearmint, lavender, rhizoma atractylodis, wormwood and sweet wormwood are selected through multi-aspect investigation, and the essential oils are composed of different organic compounds and mainly comprise alcohols, aldehydes, acids, phenols, esters, ketones and terpenes. The essential oil of spearmint is mainly composed of carvone, and other components of spearmint are limonene, pinene, myrcene, limonene, eucalyptol, menthone and the like, and the essential oil has the effects of dispelling wind, relieving exterior syndrome, diminishing inflammation, easing pain, resisting bacteria, killing insects and the like; the lavender essential oil has antioxidant, calm, smooth muscle relaxing, anticoagulant, manic-resistant, insect repellent, antifungal, sedative hypnotic, and anxiolytic effects; the atractylis lancea essential oil mainly has the functions of spasmolysis, pain relieving, antibiosis, antivirus and the like, and has certain curative effects in the aspects of resisting anoxia, protecting liver, treating gastric ulcer, regulating gastrointestinal immune system and the like; the wormwood essential oil has the effects of regulating qi and blood, expelling cold-dampness, warming channels and the like; the main component of the sweet wormwood essential oil is terpenoid chemical, and has the functions of resisting bacteria, resisting cancer, resisting oxidation, resisting inflammation, clearing heat, expelling mosquitoes and the like. The essential oils have wide application in the fields of daily necessities, food industry, medicines and the like.
The invention adopts the traditional technology to extract the essential oil and the hydrosol in the plant raw materials such as spearmint, lavender, rhizoma atractylodis, wormwood, sweet wormwood and the like, is green and pollution-free, has no solvent residue, and is also beneficial to conversion mass production. The natural active substance components are fused through the process technologies of compound addition, emulsification homogenization and the like, the spearmint hydrosol is selected as a main solvent system, and the product is rich in fresh, cool and sweet smell without adding chemical essence, so that the advantages are fully exerted. The product is mild, safe and non-irritating, does not harm the skin of a human body, has mature preparation process, less equipment investment and stable system, and can rapidly realize batch production.
Disclosure of Invention
The invention aims to provide a plant essential oil antibacterial spray product and a preparation method thereof, and the prepared product has multiple effects of bacteriostasis, moisturizing, anti-inflammation, tranquillization, skin care and the like, is applied to skin and mucous membrane sanitation, has no irritation to skin and has good system stability.
In order to achieve the above object, the technical scheme of the present invention is as follows:
the plant essential oil antibacterial spray product comprises A, B, C three-phase components in parts by weight (100 parts), wherein phase A: 0.01-5 parts of spearmint essential oil, 0.01-1 part of lavender essential oil, 0.01-1 part of atractylis lancea essential oil, 0.01-01 part of wormwood essential oil, 0.01-1 part of sweet wormwood essential oil and 0.05-10 parts of propylene glycol; and B phase: 0.01-1 part of phenoxyethanol and 0.1-0.5 part of octoxyglycerol; and C phase: 60-90 parts of spearmint hydrosol, 0.01-0.1 part of sodium citrate, 0.01-0.05 part of bronopol, 0.5-10 parts of tween 20, 0.01-0.1 part of sodium hyaluronate and 0.01-0.3 part of defoamer.
In the invention, the preparation processes of the spearmint essential oil, the spearmint hydrosol, the lavender essential oil, the atractylis lancea essential oil, the wormwood essential oil and the sweet wormwood essential oil are as follows:
weighing spearmint raw materials, placing the spearmint raw materials into a drying container, adding distilled water according to a liquid-to-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking the spearmint raw materials in 12-14 h, heating and extracting the spearmint raw materials by using an oil bath at 110 ℃ to obtain distilled volatile oil, taking out the volatile oil, continuously distilling the volatile oil to obtain water content of 2-3 h, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water and dry the volatile oil, filtering the volatile oil to obtain spearmint essential oil, and filtering the distilled water content to obtain the spearmint hydrosol.
The lavender raw material is weighed and placed in a drying container, distilled water is added according to the liquid-material ratio of 12:1 (ml/g) by using a steam distillation method, after soaking for 6-8 h, oil bath heating at 110 ℃ is carried out for 4-h, distilled volatile oil is obtained, a small amount of anhydrous sodium sulfate is added into the volatile oil for water absorption and drying, and the lavender essential oil is obtained after filtration.
Weighing rhizoma Atractylodis (Atractylodes lancea) raw materials, placing in a drying container, adding distilled water according to a liquid-to-material ratio of 12:1 (ml/g) by steam distillation, soaking for 10-12 h, heating in 110 deg.C oil bath to extract 6 h to obtain distilled volatile oil, adding small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain rhizoma Atractylodis (Atractylodes lancea) essential oil.
Weighing moxa raw materials, placing the moxa raw materials into a drying container, adding distilled water according to a liquid-to-material ratio of 12:1 (ml/g) by using a steam distillation method, soaking the moxa raw materials in 12-16 h, heating and extracting the moxa raw materials in an oil bath at 110 ℃ for 4 h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain the moxa essential oil.
Weighing herba Artemisiae Annuae raw materials, placing in a drying container, adding distilled water according to a liquid-to-material ratio of 10:1 (ml/g) by steam distillation, soaking for 8-12 h, heating in 110 deg.C oil bath to extract 4 h to obtain distilled volatile oil, adding small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain herba Artemisiae Annuae essential oil.
The preparation method of the plant essential oil antibacterial spray comprises the following steps:
(1) Mixing propylene glycol with spearmint essential oil, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in the preparation process according to parts by weight, so that the spearmint essential oil, the lavender essential oil, the atractylis lancea essential oil and the sweet wormwood essential oil are fully dissolved to prepare a phase A mixed solution;
(2) Mixing phenoxyethanol and octoxyglycerol according to parts by weight to make the phenoxyethanol and the octoxyglycerol fully compatible with each other to prepare a phase B mixed solution;
(3) Mixing the clear liquid obtained by filtering impurities of the spearmint hydrolat in the preparation process with sodium citrate, bromonitropropylene glycol, tween 20, sodium hyaluronate and defoamer according to parts by weight, and stirring for 5-30min at room temperature to obtain a phase C mixed solution;
(4) Mixing B, C two-phase mixed liquid obtained in the step (2) and (3) with each other, stirring for 30-60min at room temperature, slowly adding the A-phase mixed liquid obtained in the step (1) into the solution, stirring for 30-60min, and standing for 1-72h to obtain the antibacterial spray product.
The invention has the following advantages and beneficial effects:
(1) The essential oil and the hydrolat are extracted from spearmint, lavender, rhizoma atractylodis, wormwood and sweet wormwood, the components have definite efficacy, safety and no toxic or side effect, the natural plants are fully utilized, the resource utilization rate is improved, and the antibacterial, nerve soothing and eye-catching effects of the product are improved;
(2) The main solvent system of the invention is spearmint hydrolat, and chemical essence is not required to be added, so that the product is rich in fresh, cool, fragrant and sweet smell, and is safe and free from irritation;
(3) The preparation method of the essential oil provided by the invention is a traditional water extraction process, is simple and easy to operate, has good protection on the medicinal active ingredients in natural plants, has controllable cost, has no organic solvent residue, and is green and purely natural;
(4) The preparation technology is mature, the process is simple, the operation time is greatly shortened, the efficiency is improved, the high-end production equipment such as a homogenizer and the like is not needed, the full and uniform mixing can be realized, and the production cost is reduced;
(5) The invention realizes bacteriostasis and disinfection, and the solvent volatilizes and absorbs rapidly, and the residual substances can inhibit bacteria, disinfect, tranquilize and moisturize, so that the skin is moist and not tight, and the invention is safe and has no stimulation;
(6) The invention meets the requirements of the Ministry of health, and aims at the skin and mucous membrane of human body, and the main sterilization strains are staphylococcus aureus, pseudomonas aeruginosa and candida albicans;
(7) The invention meets the requirements of QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, and has remarkable moisturizing effect on human skin;
(8) The invention is mainly obtained by adding various plant essential oil components and solvents into spearmint hydrolat. The single plant essential oil has the functions of inhibiting bacteria and resisting inflammation, soothing nerves and calming, relieving fever and easing pain, but the main components are different in content, the antibacterial type is single, and the aging is not long. The components of the plant essential oil comprise organic compounds such as alcohols, aldehydes, acids, phenols, esters, ketones, terpenes and the like, and the synergistic effect can be achieved by the combined action of the organic compounds, so that the bactericidal activity on staphylococcus aureus, pseudomonas aeruginosa, candida albicans and the like is enhanced, the skin of a human body is stabilized, and the effects of moisturizing, relieving, diminishing inflammation and the like are achieved; the main solvent system is spearmint hydrolat, which contains a small amount of chemical components such as carvone, limonene, pinene, myrcene, menthone and the like, and has fresh smell and is refreshing; experimental results show that the plant essential oils and the hydrosol are matched for use, so that the optimal use effect can be obtained.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without any inventive effort, are intended to be within the scope of the invention.
The parts in the examples and comparative examples below represent grams.
Example 1 (product 8)
The preparation methods of the spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, wormwood essential oil and sweet wormwood essential oil in the embodiment are as follows:
weighing spearmint powder, placing the spearmint powder into a drying container, adding distilled water according to a liquid-to-material ratio of 12:1 (ml/g) by using a traditional steam distillation method, soaking 12-14 h, heating and extracting 4 h by using an oil bath at 110 ℃ to obtain distilled volatile oil, taking out the volatile oil, continuing to distill 2-3 h to obtain water content of distilled water, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water and dry, filtering to obtain spearmint essential oil, and filtering the distilled water content to obtain spearmint hydrosol; weighing Lavender powder, placing in a drying container, adding distilled water according to a liquid-material ratio of (ml/g) 1 by using a traditional steam distillation method, soaking 6-8 h, heating in an oil bath at 110 ℃ to extract 4 h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain Lavender essential oil; weighing rhizoma Atractylodis powder, placing in a drying container, adding distilled water at a liquid-to-material ratio of 12:1 (ml/g) by conventional steam distillation method, soaking 10-12 h, heating in 110 deg.C oil bath to extract 6 h to obtain distilled volatile oil, adding small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain rhizoma Atractylodis essential oil; weighing moxa raw materials, placing the moxa raw materials into a drying container, adding distilled water according to a liquid-to-material ratio of 12:1 (ml/g) by using a traditional steam distillation method, soaking the moxa raw materials in 12-16 h, heating and extracting the moxa raw materials in an oil bath at 110 ℃ for 4 h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying the volatile oil, and filtering the volatile oil to obtain the moxa essential oil; weighing herba Artemisiae Annuae raw materials, placing in a drying container, adding distilled water according to a liquid-material ratio of 10:1 (ml/g) by conventional steam distillation method, soaking for 8-12 h, heating in 110 deg.C oil bath to extract 4 h to obtain distilled volatile oil, adding small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain herba Artemisiae Annuae essential oil.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 0.5 part of lavender essential oil, 0.5 part of rhizoma atractylodis essential oil, 0.5 part of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.1 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance of the spearmint hydrolat is 100 parts of all components), sodium citrate 0.1 part, bronopol 0.01 part, tween 20 5 parts, sodium hyaluronate 0.05 part and defoamer 0.05 part, and stirring for 20min at room temperature to obtain a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 30min, slowly adding the A-phase mixed solution into the solution, stirring for 30min, and standing for 12h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', and the results show that the bacteria inhibition rate of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like is not 98.8%, 100% and 97.5%, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 11.67%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 19.86%.
Example 2
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing propylene glycol 8 parts with spearmint essential oil 3 parts, lavender essential oil 0.3 parts, rhizoma atractylodis essential oil 0.2 parts, wormwood essential oil 0.2 parts and sweet wormwood essential oil 0.2 parts in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.4 part of phenoxyethanol and 0.1 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.1 part of sodium citrate, 0.03 part of bronopol, 7 parts of tween, 0.1 part of sodium hyaluronate and 0.1 part of defoamer, and stirring for 30min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 60min, slowly adding the A-phase mixed solution into the solution, stirring for 60min, and standing for 24h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', which shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 97.8%, 99.9% and 96.8%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 11.54%; the product has a significant rise (p < 0.001) compared with the basic value for four weeks, and the rise rate is 20.01%.
Example 3
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 10 parts of propylene glycol with 4 parts of spearmint essential oil, 0.4 part of lavender essential oil, 0.2 part of rhizoma atractylodis essential oil, 0.2 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.5 part of phenoxyethanol and 0.05 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.05 part of sodium citrate, 0.01 part of bronopol, 20 parts of tween, 0.05 part of sodium hyaluronate and 0.1 part of defoamer, and stirring for 15min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 40min, slowly adding the A-phase mixed solution into the solution, stirring for 40min, and standing for 12h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', which shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 96.5%, 100% and 95.8%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 10.23%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 19.78%.
Example 4
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing propylene glycol 5 parts with spearmint essential oil 2 parts, lavender essential oil 0.3 parts, rhizoma atractylodis essential oil 0.1 parts, wormwood essential oil 0.1 parts and sweet wormwood essential oil 0.1 parts in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.5 part of phenoxyethanol and 0.01 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.05 part of sodium citrate, 0.01 part of bronopol, 20 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoamer, and stirring for 25min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 50min, slowly adding the A-phase mixed solution into the solution, stirring for 50min, and standing for 36h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', which shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 96.5%, 98.5% and 96.7%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 10.17%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 19.53%.
Example 5
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 10 parts of propylene glycol with 5 parts of spearmint essential oil, 0.3 part of lavender essential oil, 0.2 part of rhizoma atractylodis essential oil, 0.3 part of wormwood essential oil and 0.2 part of sweet wormwood essential oil in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.05 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.1 part of sodium citrate, 0.02 part of bronopol, 20 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoamer, and stirring for 30min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 30min, slowly adding the A-phase mixed solution into the solution, stirring for 60min, and standing for 72h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', and the results show that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 98.6%, 100% and 97.5%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 11.62%; the product has a significant rise (p < 0.001) compared with the basic value for four weeks, and the rise rate is 21.03%.
Example 6
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 10 parts of propylene glycol with 5 parts of spearmint essential oil, 0.4 part of lavender essential oil, 0.1 part of rhizoma atractylodis essential oil, 0.3 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.4 part of phenoxyethanol and 0.05 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.1 part of sodium citrate, 0.02 part of bronopol, 20 parts of tween, 0.05 part of sodium hyaluronate and 0.1 part of defoamer, and stirring for 5min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 60min, slowly adding the A-phase mixed solution into the solution, stirring for 30min, and standing for 10h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', and the results show that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 98.8%, 100% and 98.5%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 11.52%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 20.76%.
Example 7
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing propylene glycol 9 parts with spearmint essential oil 4 parts, lavender essential oil 0.3 parts, rhizoma atractylodis essential oil 0.1 parts, wormwood essential oil 0.2 parts and sweet wormwood essential oil 0.1 parts in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.1 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.05 part of sodium citrate, 0.01 part of bronopol, 20 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoamer, and stirring for 15min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 40min, slowly adding the A-phase mixed solution into the solution, stirring for 50min, and standing for 8h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', which shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 97.6%, 98.8% and 95.8%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 10.86%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 19.78%.
Example 8
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing propylene glycol 8 parts with spearmint essential oil 3 parts, lavender essential oil 0.3 parts, rhizoma atractylodis essential oil 0.2 parts, wormwood essential oil 0.2 parts and sweet wormwood essential oil 0.1 parts in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.2 part of phenoxyethanol and 0.05 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.1 part of sodium citrate, 0.05 part of bronopol, 20 parts of tween, 0.05 part of sodium hyaluronate and 0.1 part of defoamer, and stirring for 5min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 35min, slowly adding the A-phase mixed solution into the solution, stirring for 50min, and standing for 1h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', which shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 96.8%, 98.8% and 97.8%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 10.56%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 19.67%.
Example 9
The preparation methods of spearmint essential oil, spearmint hydrosol, lavender essential oil, atractylis lancea essential oil, mugwort essential oil and sweet wormwood essential oil in this example are the same as in example 1.
The preparation method of the plant essential oil antibacterial spray product of the embodiment comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol with 2 parts of spearmint essential oil, 0.5 part of lavender essential oil, 0.1 part of rhizoma atractylodis essential oil, 0.3 part of wormwood essential oil and 0.3 part of sweet wormwood essential oil in the preparation process to make them fully compatible to prepare a phase A mixed solution; mixing 0.3 part of phenoxyethanol and 0.05 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing spearmint hydrolat (the balance) in the preparation process with 0.1 part of sodium citrate, 0.05 part of bronopol, 20 parts of tween, 0.1 part of sodium hyaluronate and 0.05 part of defoamer, and stirring for 25min at room temperature to prepare a phase C mixed solution; mixing the B, C two-phase mixed solution, stirring at room temperature for 45min, slowly adding the A-phase mixed solution into the solution, stirring for 35min, and standing for 36h to obtain the antibacterial spray product.
The test is carried out according to the test of '2.1.8 bacteria inhibition', which shows that the antibacterial rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like are 96.5%, 98.6% and 97.8%, respectively, and the antibacterial effect is stronger.
According to the embodiment, the test is carried out according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with a basic value (43%) in one week of the use of the product, and the rising rate is 10.56%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 19.58%.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Comparative example 1 (product 1, based on the formulation of example 1, contains no atractylis lancea essential oil and no sweet wormwood essential oil)
The preparation method of the plant essential oil antibacterial spray product of the comparative example comprises the following steps (all raw materials are calculated according to 100 parts by weight):
Mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil and 1 part of wormwood essential oil in the preparation process to make the essential oil fully dissolved to prepare a phase A mixed solution; other embodiments are the same as in example 1.
The comparative example is tested according to the test of '2.1.8 bacteria inhibition', which shows that the bacteria inhibition rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans are 56.8%, 98.9% and 5.3% respectively.
The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is obviously increased (p < 0.001) compared with the basic value (43%) in one week of the use of the product, and the rising rate is 8.67%; the product has significant rise (p < 0.001) compared with the basic value after four weeks of use, and the rise rate is 10.96 percent.
Comparative example 2 (product 2, based on the formulation of example 1, is free of lavender essential oil, spearmint essential oil and mugwort essential oil)
The preparation method of the plant essential oil antibacterial spray product of the comparative example comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol, 3.5 parts of atractylis lancea essential oil and 1.5 parts of sweet wormwood essential oil in the preparation process, and fully dissolving to prepare a phase A mixed solution; other embodiments are the same as in example 1.
The comparative example is tested according to the test of '2.1.8 bacteria inhibition', which shows that the bacteria inhibition rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans are 4.5%, 98.6% and 58.1% respectively.
The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with the basic value (43%) in one week of the use of the product, and the rising rate is 7.83%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 9.76%.
Comparative example 3 (product 3, based on the formulation of example 1, without atractylis lancea essential oil)
The preparation method of the plant essential oil antibacterial spray product of the comparative example comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil, 0.5 part of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to make the components fully dissolved to prepare a phase A mixed solution; other embodiments are the same as in example 1.
The comparative example is tested according to the test of '2.1.8 bacteria inhibition', which shows that the bacteria inhibition rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans are 60.5%, 99.9% and 20.8% respectively.
The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is obviously increased (p < 0.001) compared with the basic value (43%) in one week of the use of the product, and the rising rate is 9.39%; the product has a significant rise (p < 0.001) compared with the basic value for four weeks, and the rise rate is 10.97%.
Comparative example 4 (product 4, based on the formulation of example 1, without sweet wormwood essential oil)
The preparation method of the plant essential oil antibacterial spray product of the comparative example comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil, 0.5 part of atractylis lancea essential oil and 0.5 part of wormwood essential oil in the preparation process to make them fully dissolved to prepare a phase A mixed solution; other embodiments are the same as in example 1.
The comparative example is tested according to the test of '2.1.8 bacteria inhibition', which shows that the bacteria inhibition rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans are 61.1%, 99.9% and 28.5% respectively.
The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with the basic value (43%) in one week of the use of the product, and the rising rate is 10.58%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 11.93%.
Comparative example 5 (product 5, based on the formulation of example 1, without lavender essential oil)
The preparation method of the plant essential oil antibacterial spray product of the comparative example comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of atractylis lancea essential oil, 0.5 part of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to make the components fully dissolved to prepare a phase A mixed solution; other embodiments are the same as in example 1.
The comparative example is tested according to the test of '2.1.8 bacteria inhibition', which shows that the bacteria inhibition rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans are 48.6%, 99.9% and 60.1% respectively.
The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with the basic value (43%) in one week of the use of the product, and the rising rate is 10.10%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 10.56%.
Comparative example 6 (product 6, based on the formulation of example 1, without spearmint essential oil)
The preparation method of the plant essential oil antibacterial spray product of the comparative example comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol with 1.5 parts of lavender essential oil, 1.5 parts of atractylis lancea essential oil, 1.5 parts of wormwood essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to make them fully dissolved to prepare a phase A mixed solution; other embodiments are the same as in example 1.
The comparative example is tested according to the test of '2.1.8 bacteria inhibition', which shows that the bacteria inhibition rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans are 45.8%, 99.9% and 59.5% respectively.
The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is obviously increased (p < 0.001) compared with the basic value (43%) in one week of the use of the product, and the rising rate is 9.61%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 11.05%.
Comparative example 7 (product 7, based on the formulation of example 1, without mugwort essential oil)
The preparation method of the plant essential oil antibacterial spray product of the comparative example comprises the following steps (all raw materials are calculated according to 100 parts by weight):
mixing 7 parts of propylene glycol with 3 parts of spearmint essential oil, 1 part of lavender essential oil, 0.5 part of atractylis lancea essential oil and 0.5 part of sweet wormwood essential oil in the preparation process to make the components fully dissolved to prepare a phase A mixed solution; other embodiments are the same as in example 1.
The comparative example is tested according to the test of '2.1.8 bacteria inhibition', which shows that the bacteria inhibition rates of the test items such as pseudomonas aeruginosa, staphylococcus aureus, candida albicans are 48.8%, 99.9% and 59.8% respectively.
The comparative example is tested according to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines, the test environment temperature is 20.1-21.4 ℃, the relative humidity is 44.0-55.3%, the age of 32 subjects is 25-52 years old, the average age is 38.44 +/-1.36 years old, each subject uses a test sample according to the requirement in a specified time, the four sides of the product are continuously used on the premise that no adverse reaction occurs according to the adverse reaction recording result, the moisture content of skin horny layer of cheek is remarkably increased (p < 0.001) compared with the basic value (43%) in one week of the use of the product, and the rising rate is 10.54%; the product has a significant rise (p < 0.001) compared with the base value for four weeks, and the rise rate is 11.86%.
The antibacterial spray products prepared in comparative examples 1-7 and example 1 were compared with the existing antibacterial spray products of a certain brand by volunteer trial, and the experimental results are shown in the following table:
experiments show that the plant essential oil antibacterial spray product has a very strong antibacterial effect and a very obvious moisturizing effect. Among different plant essential oils, the combination of spearmint essential oil, lavender essential oil and wormwood essential oil has a strong inhibition effect on pseudomonas aeruginosa, and the inhibition rate of single use or the combination of any two essential oils is less than 50%, but the inhibition effect of the three essential oils on candida albicans is weak; the atractylis lancea essential oil and sweet wormwood essential oil combined use has a strong antibacterial effect on candida albicans, the antibacterial rate is less than 50% when the atractylis lancea essential oil and sweet wormwood essential oil are singly used, and the two essential oils have weak inhibition effect on pseudomonas aeruginosa; the five essential oils have the strongest inhibition effect on staphylococcus aureus, the inhibition rate can reach more than 90%, and the inhibition rate of three bacteria can reach more than 90% at the same time, so that the five essential oils are used in combination, and the inhibition effect is better and obvious; the essential oils contain volatile components of alcohols, aldehydes, acids, phenols, esters, ketones, terpenes and other organic compounds, have good antioxidant, anti-inflammatory, nursing and moisturizing effects on human skin, and can ease skin function and increase skin moisture content more than that of a single essential oil.
The foregoing has shown and described the basic principles and main features of the present invention, as well as the advantages and benefits of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (1)
1. A preparation method of a plant essential oil antibacterial spray is characterized by comprising the following steps of:
all raw materials are mixed with each other by 100 parts by weight of 10 parts of propylene glycol, 5 parts of spearmint essential oil, 0.4 part of lavender essential oil, 0.1 part of rhizoma atractylodis essential oil, 0.3 part of wormwood essential oil and 0.1 part of sweet wormwood essential oil, so that the raw materials are fully dissolved to prepare a phase A mixed solution; mixing 0.4 part of phenoxyethanol and 0.05 part of octoxyglycerol to make them fully compatible to obtain phase B mixed solution; mixing 0.1 part of sodium citrate, 0.02 part of bromonitropropylene glycol, 5 parts of tween, 0.05 part of sodium hyaluronate, 0.1 part of defoamer and the balance of spearmint pure dew with each other, and stirring for 5min at room temperature to obtain a phase C mixed solution, wherein the spearmint pure dew is spearmint pure dew obtained in the preparation process of spearmint essential oil; mixing the B, C two-phase mixed solution, stirring at room temperature for 60min, slowly adding the A-phase mixed solution into the solution, stirring for 30min, and standing for 10h to obtain plant essential oil antibacterial spray;
The preparation method of the spearmint essential oil, spearmint hydrosol, lavender essential oil, rhizoma atractylodis essential oil, wormwood essential oil and sweet wormwood essential oil comprises the following steps:
weighing spearmint powder, placing the spearmint powder into a drying container, adding distilled water according to a liquid-to-material ratio of ml/g=12:1 by using a traditional steam distillation method, soaking 12-14 h, heating and extracting 4 h by using an oil bath at 110 ℃ to obtain distilled volatile oil, taking out the volatile oil, continuing to distill 2-3 h to obtain distilled water, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water and dry, filtering to obtain spearmint essential oil, and filtering the distilled water to obtain spearmint hydrosol;
weighing Lavender powder, placing in a drying container, adding distilled water according to a liquid-to-material ratio of ml/g=12:1 by using a traditional steam distillation method, soaking 6-8 h, heating in an oil bath at 110 ℃ to extract 4 h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain Lavender essential oil;
weighing rhizoma Atractylodis powder, placing in a drying container, adding distilled water according to a liquid-to-material ratio of ml/g=12:1 by conventional steam distillation method, soaking 10-12 h, heating in 110 deg.C oil bath to extract 6 h to obtain distilled volatile oil, adding small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain rhizoma Atractylodis essential oil;
Weighing moxa raw materials, placing the moxa raw materials into a drying container, adding distilled water according to a liquid-material ratio of ml/g=12:1 by using a traditional steam distillation method, soaking the moxa raw materials in 12-16 h, heating and extracting the moxa raw materials in an oil bath at 110 ℃ for 4 h to obtain distilled volatile oil, adding a small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying the volatile oil, and filtering the volatile oil to obtain the moxa essential oil;
weighing herba Artemisiae Annuae raw materials, placing in a drying container, adding distilled water according to a liquid-material ratio of ml/g=10:1 by conventional steam distillation method, soaking 8-12 h, heating in 110 deg.C oil bath to extract 4 h to obtain distilled volatile oil, adding small amount of anhydrous sodium sulfate into the volatile oil to absorb water, drying, and filtering to obtain herba Artemisiae Annuae essential oil.
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