CN115136968A - Natural preservative containing camellia oleifera extract and preparation method thereof - Google Patents
Natural preservative containing camellia oleifera extract and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
Abstract
The invention relates to the technical field of preservatives, and particularly discloses a natural preservative containing an oil tea extract and a preparation method thereof. The preservative comprises the following raw materials in percentage by mass: 40 to 50 percent of camellia oleifera extract, 35 to 50 percent of traditional Chinese medicine extract, 0.02 to 8 percent of tea polyphenol and 0.03 to 2 percent of chitin; the Camellia oleifera extract is extract of Camellia oleifera leaves, camellia oleifera flowers and Camellia oleifera shell core. The Chinese medicinal extract is extract of flos Lonicerae, caulis Lonicerae, pericarpium Granati, folium Artemisiae Argyi, perillae herba, radix Sophorae Flavescentis, coptidis rhizoma, cortex moutan and herba Artemisiae Annuae. The natural preservative prepared by the synergistic effect of the components overcomes the allergen risk of the traditional chemical preservative, can reduce or delay the growth and the propagation of microorganisms, can be used as the preservative in industries such as cosmetics, washing and caring products, foods and the like to delay the putrefaction caused by the growth or the chemical change of the microorganisms and inhibit the growth and the propagation of the microorganisms so as to prolong the storage time of articles and inhibit the putrefaction of substances, and has the effects of being natural, safe, non-toxic, harmless and non-irritant.
Description
Technical Field
The invention relates to the technical field of preservatives, in particular to a natural preservative containing an oil tea extract and a preparation method thereof.
Background
With the advent of the consumer appeal of "natural, organic, chemically non-preserved", traditional chemically synthetic preservatives, which have some toxicity due to structure and properties, have been gradually replaced by safer preservatives. For example, the imidazolidinyl urea series, the hydantoin derivatives dmdmdmh (formaldehyde donor), isothiazolinone, parabens (paraben), bronopol, quaternary ammonium salt-15, kaempon-CG, triclosan and the like, have good antiseptic effect and low cost, but have large skin irritation, and some of them are even carcinogenic and allergenic, and thus have gradually become replaced by new natural preservatives in cosmetics and personal care products. Most of the reported natural preservatives are derived from natural organisms, including animal resources, plant resources, microorganisms and the like.
Natural antiseptic substances can be extracted from many plants in nature. In recent years, people begin to extract effective components from plants or traditional Chinese medicines, the effective components have natural antibacterial action, for example, the extract in the sweet wormwood herb has certain antibacterial activity, the tea polyphenol has good antiseptic and fresh-keeping effects, and the extract has the inhibitory effects on bacillus subtilis, staphylococcus aureus, escherichia coli, tomato ulcer, streptococcus carious, mucor, penicillium, gibberella, anthrax and beer yeast. Because different kinds of plant extracts have respective antibacterial spectrum and application range, and a single component cannot achieve the comprehensive preservative effect, the synergistic effect of multiple natural antibacterial components is often needed to achieve better preservative capability. Therefore, how to match different antibacterial ingredients to achieve a synergistic preservative effect is a difficult point in developing novel natural preservatives at present.
The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.
Disclosure of Invention
The invention aims to provide a natural preservative containing an oil tea extract and a preparation method thereof. The invention takes the camellia oleifera extract, the traditional Chinese medicine extract, the tea polyphenol and the chitin as raw materials, and the antibacterial synergistic effect exists among the extracts, so the invention has obvious antibacterial and antiseptic effects and can be widely applied as a natural preservative.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a natural preservative containing an oil tea extract is composed of the following raw materials in percentage by mass: 40 to 50 percent of camellia oleifera extract, 35 to 50 percent of traditional Chinese medicine extract, 0.02 to 8 percent of tea polyphenol and 0.03 to 2 percent of chitin (chitosan/chitosan oligosaccharide); the Camellia oleifera extract is extract of Camellia oleifera leaf, camellia oleifera flower and Camellia oleifera shell core. The traditional Chinese medicine extract is extracts of honeysuckle flower, honeysuckle flower stem, pomegranate rind, folium artemisiae argyi, purple perilla, radix sophorae flavescentis, coptis chinensis, peony root bark and sweet wormwood herb.
The scheme of the invention is further optimized as follows: the chitin comprises chitosan and/or chitosan oligosaccharide.
The scheme of the invention is further optimized as follows: the mass ratio of the camellia oleifera leaves, the camellia oleifera flowers and the camellia oleifera shell cores in the extract is 1.
The scheme of the invention is further optimized as follows: the traditional Chinese medicine extract comprises extracts of honeysuckle flower, honeysuckle flower stem, pomegranate rind, folium artemisiae argyi, purple perilla, radix sophorae flavescentis, coptis chinensis, peony root bark and sweet wormwood in equal mass ratio.
The invention also provides a preparation method of the natural preservative containing the camellia oleifera extract, which comprises the following steps:
(1) Preparing a camellia oleifera extract: picking oil tea leaves, oil tea flowers and oil tea shell cores, deactivating enzymes, crushing, extracting with ethanol twice, mixing the extract solutions, centrifuging, collecting supernatant, performing rotary evaporation, and concentrating to solid content of 50-80% to obtain oil tea extract;
(2) Preparing a traditional Chinese medicine extract: pulverizing Chinese medicinal materials, decocting, cooling, extracting with ethanol twice, centrifuging, collecting supernatant, rotary evaporating, and concentrating to solid content of 50-80% to obtain Chinese medicinal extract;
(3) The camellia oleifera extract and the traditional Chinese medicine extract are uniformly mixed according to a ratio, spray drying, vacuum-pumping and storing are carried out to obtain mixed powder, and finally the mixed powder, tea polyphenol and chitin are prepared according to a ratio to obtain the natural preservative.
The scheme of the invention is further optimized as follows: the ethanol leaching in the step (1) and the step (2) is performed for 5 to 24 hours by adopting 50 to 75 percent at the temperature of between 20 and 35 ℃.
The scheme of the invention is further optimized as follows: the rotary evaporation is carried out at a temperature of 40-50 ℃.
The scheme of the invention is further optimized as follows: and (3) deactivating enzyme for 1-2min by 100W microwave before crushing the materials in the step (1) and the step (2).
The scheme of the invention is further optimized as follows: and (3) the cooking in the step (2) is performed for 30-60min in a water bath kettle at 100 ℃.
Compared with the prior art, the invention has the advantages and beneficial effects that:
1. the camellia oleifera extract, the traditional Chinese medicine extract, the tea polyphenol and the chitin are used as raw materials, the camellia oleifera extract, the traditional Chinese medicine extract, the tea polyphenol and the chitin can be used for anticorrosion in the fields of cosmetics, food and the like, the synergistic effect of antibacterial property also exists among the extracts, the antibacterial and anticorrosion effects are obvious, and the camellia oleifera extract, the traditional Chinese medicine extract, the tea polyphenol and the chitin can be widely used as natural preservatives.
2. The camellia extract, the traditional Chinese medicine extract and other natural plant components are used as main raw materials, the allergen risk of the traditional chemical preservative is overcome, the growth and the propagation of microorganisms can be reduced or delayed, the camellia extract, the traditional Chinese medicine extract and other natural plant components can be used as preservatives in industries such as cosmetics, washing products, foods and the like, the corruption caused by the growth or the chemical change of the microorganisms is delayed, the growth and the propagation of the microorganisms are inhibited, the storage time of articles is prolonged, and the preparation for inhibiting substance corruption has natural and safe effects.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A natural preservative containing an oil tea extract is composed of the following raw materials in percentage by mass: 50% of camellia oleifera extract, 45% of traditional Chinese medicine extract, 3% of tea polyphenol and 2% of chitosan oligosaccharide, and the preparation method of the natural preservative comprises the following steps:
(1) Preparing a camellia oleifera extract: picking oil tea leaves, oil tea flowers and oil tea shell cores, deactivating enzymes for 1-2min by 100W, crushing to 60 meshes, extracting with 50-75% ethanol, extracting at 20-35 ℃ for 5-24h, repeating the extraction twice, combining the extract, centrifuging, taking supernatant, performing rotary evaporation at 40-50 ℃, and concentrating until the solid content reaches 50-80% to obtain an oil tea extract;
(2) Preparing a traditional Chinese medicine extract: pulverizing Chinese medicinal materials (flos Lonicerae, caulis Lonicerae, pericarpium Granati, folium Artemisiae Argyi, perillae herba, radix Sophorae Flavescentis, coptidis rhizoma, cortex moutan and herba Artemisiae Annuae with equal mass), steaming and decocting in 100 deg.C water bath for 30-60min, cooling, leaching with 50-75% at 20-35 deg.C for 5-24 hr, repeating leaching for 2 times, mixing leaching solutions, centrifuging, collecting supernatant, rotary evaporating at 40-50 deg.C, and concentrating to solid content of 50-80% to obtain Chinese medicinal extract;
(3) Uniformly mixing 50g of camellia oleifera extract and 45g of traditional Chinese medicine extract according to a ratio, performing spray drying, extracting and vacuum storing to obtain mixed powder, and finally preparing the mixed powder, 3g of tea polyphenol and 2g of chitosan oligosaccharide according to a ratio to obtain the natural preservative.
Example 2
A natural preservative containing an oil tea extract is composed of the following raw materials in percentage by mass: 50% of camellia oleifera extract, 40% of traditional Chinese medicine extract, 8% of tea polyphenol and 2% of chitosan.
The preparation method of the natural preservative comprises the following steps:
(1) Preparing a camellia oleifera extract: picking oil tea leaves, oil tea flowers and oil tea shell cores, deactivating enzymes for 1-2min by 100W, crushing to 60 meshes, extracting with 50-75% ethanol, extracting at 20-35 ℃ for 5-24h, repeating the extraction twice, combining the extract, centrifuging, taking supernatant, performing rotary evaporation at 40-50 ℃, and concentrating until the solid content reaches 50-80% to obtain an oil tea extract;
(2) Preparing a traditional Chinese medicine extract: pulverizing Chinese medicinal materials (flos Lonicerae, caulis Lonicerae, pericarpium Granati, folium Artemisiae Argyi, perillae herba, radix Sophorae Flavescentis, coptidis rhizoma and herba Artemisiae Annuae with equal mass), steaming and decocting in 100 deg.C water bath for 30-60min, cooling, leaching with 50-75% at 20-35 deg.C for 5-24 hr, repeating leaching for 2 times, mixing leaching solutions, centrifuging, collecting supernatant, rotary evaporating at 40-50 deg.C, and concentrating to solid content of 50-80% to obtain Chinese medicinal extract;
(3) Uniformly mixing 50g of camellia oleifera extract and 40g of traditional Chinese medicine extract according to a ratio, performing spray drying, extracting and vacuum storing to obtain mixed powder, and finally preparing the mixed powder, 8g of tea polyphenol and 2g of chitosan according to a ratio to obtain the natural preservative.
Example 3
A natural preservative rich in camellia oleifera extract is prepared from the following raw materials in percentage by mass: 50% of camellia oleifera extract, 43% of traditional Chinese medicine extract, 6% of tea polyphenol and 1% of chitosan oligosaccharide;
the preparation method of the natural preservative comprises the following steps:
(1) Preparing a camellia oleifera extract: picking oil tea leaves, oil tea flowers and oil tea shell cores, deactivating enzymes for 1-2min by 100W, crushing to 60 meshes, extracting with 50-75% ethanol, extracting at 20-35 ℃ for 5-24h, repeating the extraction twice, combining the extract, centrifuging, taking supernatant, performing rotary evaporation at 40-50 ℃, and concentrating until the solid content reaches 50-80% to obtain an oil tea extract;
(2) Preparing a traditional Chinese medicine extract: pulverizing Chinese medicinal materials (flos Lonicerae, caulis Lonicerae, pericarpium Granati, folium Artemisiae Argyi, perillae herba, radix Sophorae Flavescentis, coptidis rhizoma and herba Artemisiae Annuae with equal mass), steaming and decocting in 100 deg.C water bath for 30-60min, cooling, leaching with 50-75% at 20-35 deg.C for 5-24 hr, repeating leaching for 2 times, mixing leaching solutions, centrifuging, collecting supernatant, rotary evaporating at 40-50 deg.C, and concentrating to solid content of 50-80% to obtain Chinese medicinal extract;
(3) Uniformly mixing 50g of camellia oleifera extract and 43g of traditional Chinese medicine extract according to a ratio, performing spray drying, extracting and vacuum preserving to obtain mixed powder, and finally preparing the mixed powder, 6g of tea polyphenol and 1g of chitosan oligosaccharide according to a ratio to obtain the natural preservative.
Comparative example 1
The present example differs from example 1 in that: the preservative does not contain chitosan oligosaccharide, and consists of 50g of camellia oleifera extract, 45g of traditional Chinese medicine extract and 3g of tea polyphenol.
Comparative example 2
The present example differs from example 1 in that: the preservative does not contain traditional Chinese medicine extracts, and consists of 50g of camellia oleifera extract, 3g of tea polyphenol and 2g of chitosan oligosaccharide.
Comparative example 3
The present example differs from example 1 in that: the preservative does not contain tea polyphenol, and consists of 50g of camellia oleifera extract, 45g of traditional Chinese medicine extract and 2g of chitosan oligosaccharide.
The preservative prepared in example 1 was diluted in the following concentrations, and the test results of the bacteriostatic ring test by the coating method after dilution are shown in table 1.
Table 1: test results of the antibacterial Ring of the preservative of the present invention
Note: (-) for sterile colony growth and (+) for colony growth
The preservatives prepared in examples 1 to 3 of the present invention and comparative examples were diluted at a concentration of 5% and then subjected to bacteriostatic test according to the above-mentioned method, and the test results are shown in table 2.
The preservative prepared in example 1 was subjected to an acute oral toxicity test, a skin irritation test and a skin allergy test, and the test results are shown in tables 3, 4 and 5, and the detailed test methods were as follows: 1. acute oral toxicity test: healthy rats (180 g-220 g) are selected, and the weight difference between the individuals of the same sex experimental animals is not more than 20% of the average weight after 14 days of the experiment. Rats are acclimatized in the test environment for at least 3-5 days prior to testing. 4-5 dosage groups are provided, and each group contains 10 animals, and the animals are male and female. The dose of 5000mg/kg did not cause death of the animals. 2. Skin irritation test and multiple skin irritation tests: no less than 4 rabbits are selected per group. The preservative is directly applied to the skin preparation part by adopting a one-piece left-right self-contrast method, and the dressing is covered and fixed. The application time is at least 4 hours. Multiple administrations of the skin irritation test were applied to the same area continuously, each administration was carried out for the same period of 14 days. The degree of skin damage in the damaged skin test is limited to damage of the epidermis layer. The skin reactions were observed visually under natural light or full spectrum light. Single-dose skin irritation test observation time points were 30-60 minutes, 24, 48, and 72 hours after preservative removal. Multiple skin irritation tests were performed 1 hour after each preservative removal and before each preservative application, and 30-60 minutes, 24, 48, and 72 hours after the preservative removal for the last application. The negative control animals are 20 white guinea pigs, half male and female animals, 250-300 g weight, 10 animals in each group, and are adapted to be raised in the laboratory for one week. The positive sensitizer is 2,4-dinitrochlorobenzene, and the sensitization concentration and the excitation concentration are respectively 10mg/mL and 8mg/mL.
Table 3: acute oral toxicity test results
Table 4: results of skin irritation test
From the above results, it can be seen that the antiseptic of the present invention is non-irritating to the skin of rabbits after several skin irritation tests. No irritation reaction such as erythema, edema and the like appears on the tested skin 24 hours and 48 hours after the rabbits of the test group are stimulated to contact the mixture, and the skin sensitization rate is 0 percent; no abnormality was found in the skin of the negative control guinea pigs.
Table 5: multiple skin irritation test results
Note: * The positive control group used 2.4-dinitrochlorobenzene as a positive substance (sensitization and excitation concentrations were 10mg/mL and 8mg/mL, respectively).
As can be seen from the test results, the skin allergy test results of the antiseptic of this example to guinea pigs showed that no skin allergy was observed.
The foregoing is a further detailed description of the invention in connection with specific/preferred embodiments and it is not intended to limit the invention to the specific embodiments described. It will be apparent to those skilled in the art that various substitutions and modifications can be made to the described embodiments without departing from the spirit of the invention, and such substitutions and modifications are to be considered as within the scope of the invention.
Claims (9)
1. A natural preservative containing an oil tea extract is characterized in that: the composite material comprises the following raw materials in percentage by mass: 40 to 50 percent of camellia oleifera extract, 35 to 50 percent of traditional Chinese medicine extract, 0.02 to 8 percent of tea polyphenol and 0.03 to 2 percent of chitin; the camellia oleifera extract is an extract of camellia oleifera leaves, camellia oleifera flowers and camellia oleifera shell cores,
the Chinese medicinal extract is extract of flos Lonicerae, caulis Lonicerae, pericarpium Granati, folium Artemisiae Argyi, perillae herba, radix Sophorae Flavescentis, coptidis rhizoma, cortex moutan and herba Artemisiae Annuae.
2. The natural preservative containing camellia oleifera extract according to claim 1, characterized in that: the traditional Chinese medicine extract comprises honeysuckle flower, honeysuckle flower stem, pomegranate rind, folium artemisiae argyi, purple perilla, radix sophorae flavescentis, coptis chinensis, peony root bark and sweet wormwood in equal mass ratio.
3. The natural preservative of the camellia oleifera extract according to claim 1, wherein: the chitin comprises chitosan and/or chitosan oligosaccharide.
4. The natural preservative of the camellia oleifera extract according to claim 1, wherein: the mass ratio of the camellia oleifera leaves, the camellia oleifera flowers and the camellia oleifera shell cores in the extract is 1.
5. The method for preparing a natural preservative containing an camellia sinensis extract as set forth in any one of claims 1 to 4, comprising the steps of:
preparing a camellia oleifera extract: picking oil-tea camellia tea leaves, oil-tea camellia flowers and oil-tea camellia shell cores, deactivating enzymes, crushing, repeatedly leaching twice with ethanol, combining leaching solutions, centrifuging, taking supernate, performing rotary evaporation, and concentrating until the solid content reaches 50-80% to obtain an oil-tea camellia extract;
preparing a traditional Chinese medicine extract: pulverizing Chinese medicinal materials, decocting, cooling, extracting with ethanol, repeating the extraction twice, mixing extractive solutions, centrifuging, collecting supernatant, rotary evaporating, and concentrating to solid content of 50-80% to obtain Chinese medicinal extract;
the camellia oleifera extract and the traditional Chinese medicine extract are uniformly mixed according to a ratio, spray drying and vacuum storage are carried out to obtain mixed powder, and finally the mixed powder, tea polyphenol and chitin are prepared according to a ratio to obtain the natural preservative.
6. The method for preparing a natural preservative containing a camellia oleifera extract according to claim 5, wherein: the ethanol leaching in the step (1) and the step (2) is performed for 5 to 24 hours by adopting 50 to 75 percent at the temperature of between 20 and 35 ℃.
7. The method for preparing a natural preservative containing a camellia oleifera extract according to claim 5, wherein: the rotary evaporation is carried out at a temperature of 40-50 ℃.
8. The method for preparing a natural preservative containing a camellia oleifera extract according to claim 5, wherein: and (3) deactivating enzyme for 1-2min by 100W microwave before crushing the materials in the step (1) and the step (2).
9. The method for preparing a natural preservative containing a camellia oleifera extract according to claim 5, wherein: and (3) the cooking in the step (2) is performed for 30-60min in a water bath kettle at 100 ℃.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115486478A (en) * | 2022-10-09 | 2022-12-20 | 江苏天美健大自然生物工程有限公司 | Preservative composition for protein beverage and preparation method thereof |
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