CN109907308A - Toxin-expelling and face nourishing, the preparation method and application for supplementing energy food and medicine consangunity product - Google Patents
Toxin-expelling and face nourishing, the preparation method and application for supplementing energy food and medicine consangunity product Download PDFInfo
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Abstract
The invention discloses a kind of with toxin-expelling and face nourishing, supplements the preparation method and application of energy food and medicine consangunity product.The preparation method of the product is the following steps are included: Chinese medicine mixes after being crushed by ultralow temperature with special biologos powder according to a certain percentage to obtain the final product.A kind of toxin-expelling and face nourishing, supplement energy food and medicine consangunity product can be processed into the various dosage forms such as tablet, capsule, pulvis, for food, health care product and other food and medicine consangunity product scopes relevant to health is improved.
Description
Technical field
The present invention relates to a kind of field of health care products more particularly to toxin-expelling and face nourishings, the system for supplementing energy food and medicine consangunity product
Preparation Method and application.
Background technique
It is theoretical according to Chinese medicine syndrome differentiation of yin-yang, common yin card, yang syndrome disease and its relevant administration are subjected to induction and conclusion, really
Determine 390 kinds of conventional Chinese medicine, including be served only for yin card 84 kinds of disease medicinal material, be served only for 82 kinds of yang syndrome disease medicinal material, Yin-yang Syndrome
There are 224 kinds of medicinal material used.
It is as follows to be served only for yang syndrome disease medicinal material (82 kinds):
Styrax, ardisia japonica, gingko, radix cynanchi atrati, kaolinite, cortex dictamni, chinaroot greenbrier, stephanotis, the Chinese bulbul, radix ampelopsis, Bi Collettii, Bi
Clear eggplant, fiveleaf akebia fruit, thorn rose, Chinese toon root white skin, cudrania root, fleabane flower, chaulmoogra, folium isatidis, lophatherum gracile, bitter citrus immature flower, Fermented Soybean, winter
Certain herbaceous plants with big flowers, Chinese violet, Rabdosia rubescens, Stephania epigaea, folium sennae, the root of fangji, phoenix-tail fern, fructus choerospondiatis, Caraway, galangal, cuttlebone, ligusticum sinense oliver, lotus
Leaf, airpotato yam, Lygodium japonicum, Sargent gloryvine, bryozoatum, Chinese knotweed, turmeric, herba lysimachiae, the seed of garden balsam, kuh-seng, Ilex Latifolia Thunb, radix gentianae, sieve
Cloth sesame slices, Radix zanthoxyli, serissa serissoide, Artemisia anomala, Longtube Ground Ivy Herb, armeniaca mume, ilex pubescens, gadfly, terminaliae billericae,fructus, fructus viticis, chlorite schist, big gun
Ginger, calculus bovis factitius, storax, cornu bubali, ginger, Chinese mahonia, black lotus seed, tabasheer, Rhizoma Gastrodiae, folium cycas, is led to seven cun of gold
Grass, ilexrotunda, zaocys dhumnade, Chinese parasol tree skin, the seed of cowherb, blood clam, dragon's blood, barberry skin, citron, coriander seed, China rose, emblic
Son, pappy shell, brush-cherry seed, Fructus Aurantii, cape jasmine, crinis trachycarpi.
It is as follows to be served only for yin card disease medicinal material (84 kinds):
Folium artemisiae argyi, Sculellaria barbata, crystobalite, root of Japanese banana, alum, rhizoma atractylodis, rde bean, the achene of Siberian cocklebur, tribulus terrestris, the bark of the root or stem of the tree of heaven, tsaoko,
Semen Leonuri, stringy stonecrop, Paper mulberry Fruit, the fruit of summer cypress, garlic, cordyceps sinensis, catechu, radix saposhnikoviae, Fructus Tritici Levis, affine cudweed, rhizoma cibotii, bone are broken
Benefit, downy rosemyrtle root, beggar-ticks, dog bone, dog testes and penis, walnut, Semen Juglandis, faenum graecum, semen sojae atricolor, Semen sesami nigrum, black tiger, hippocampus, sea otter, chaste tree
Mustard, semen allii tuberosi, Canton love-pea vine, Root of Taipei Monkshood, broom top, semen litchi, arillus longan, Siraitia grosvenorii, calamine, geranium wilfordii, pawpaw, Strychnos nux-vomica
Son, caulis akebiae, radix ranunculi ternati, Lasiosphaera fenzlii, butterflybush flower, beautiful millettia root pizzle, the sterculia seed, Gorgon fruit, philippine flemingia root, the seed of feather cockscomb, ramulus mori, cynomorium songaricum, sand
Yuan Zi, granatum, frutus cnidii, mulberries, mantis egg-case, subprostrate sophora, lucid asparagus, Radix aconiti szechenyiani, the root of three-nerved spicebush, cortex acanthopanacis, evergreen, Wangjiang
South, winged euonymus, thizoma curculiginis, fructus alpiniae oxyphyllae, bat's faces, actinolite, sheep bone, caulis bambusae in taenian, dried human placenta, fructus gleditsiae, creeping oxalis, stalactite, paris polyphylla,
Japanses beauty-berry grass, native copper.
Yin-yang Syndrome shared herbal medicines (224 kinds) are as follows:
The root of Dahurain angelica, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, the tuber of pinellia, psoralea corylifolia, the bletilla striata, borneol, Cynanchum glaucescens, lily, Zi Ren, fritillaria, Morinda officinalis, hyacinth bean,
The tuber of stemona, peppermint, Radix Isatidis, betel nut, turtle shell, the larva of a silkworm with batrytis, semen brassicae, field pennycress, pivot storage, oldenlandia diffusa, rhizoma imperatae, dried orange peel,
Agalloch eaglewood, radix paeoniae rubra, cicada slough, wilsonii, semen plantaginis, Rhizoma Chuanxiong, radix bupleuri, Fructus meliae toosendan, magnetite, red halloysite, the dried venom of toads, pangolin, cacumen biotae,
Silkworm excrement, jujube, cloves, Radix Angelicae Sinensis, Radix Codonopsis, glutinous rehmannia, rheum officinale, pheretima, Cortex Eucommiae, garden burnet, Radix Salviae Miltiorrhizae, Radix Angelicae Pubescentis, the shell of areca nut, rush, generation
Ochre, Semen Benincasae, the root bark of Chinese wolf-berry, curcuma zedoary, donkey-hide gelatin, Poria cocos, monkshood, raspberry, fingered citron, Radix Glycyrrhizae, rhizoma zingiberis, ramulus cinnamomi, Snakegourd Fruit, fructus lycii,
Gekko, pueraria lobata, tortoise, uncaria, rhizoma nardostachyos, Radix Astragali, Cortex Magnoliae Officinalis, safflower, polygonum cuspidate, radix scutellariae, wrinkled giant hyssop, the coptis, the fleece-flower root, red ginseng, root of kirilow rhodiola,
Rhizoma polygonati, Cortex Phellodendri, myrobalan, gypsum rubrum, seaweed, talcum, amber, cortex albiziae, Eclipta prostrata, sophora flower, silky ant, dalbergia wood, Caulis Spatholobi, tangerine
The red, fruit of Cherokee rose, campanulaceae, endothelium corneum gigeriae galli, honeysuckle, chrysanthemum, cassia seed, gynostemma pentaphylla, centella, aspongopus, thallus laminariae, tussilago, company
It sticks up, reed root, keel, radish seed, stamen nelumbini, antelope's horn, ganoderma lucidum, Fructus Liquidambaris, malt, radix aucklandiae, Radix Ophiopogonis, purslane, edestan, tree peony
Skin, Huang, oyster, myrrh, saltcake, rose, Oroxylum indicum, radix achyranthis bidentatae, great burdock achene, the fruit of glossy privet, cattail pollen, dandelion, loguat leaf, pendant
Orchid, scorpio, green peel, the root of purple-flowered peucedanum, Rhizoma Et Radix Notopterygii, the bark of ash, schefflera arboricola, sweet wormwood, indigo naturalis, madder, ginseng, cortex cinnamomi, olibanum, Herba Cistanches, meat
Cardamom, caulis lonicerae, hawthorn, fructus amomi, semen ziziphi spinosae, Radix Notoginseng, Moschus, leech, Fructus Corni, Chinese yam, dendrobium nobile, the root of straight ladybell, mulberry leaf, stone Chang
Pu, herba taxilli, the root bark of white mulberry, cimicifugae foetidae, trigone, gypsum, blackberry lily, Divine Comedy, edible tulip, the calyx and receptacle of a persimmon, pyrrosia lingua, sea-buckthorn, the shell of seaear, pine needle,
Ground bettle, santal, peach kernel, radices trichosanthis, Semen Cuscutae, lepidium seed, radix pseudostellariae, rhizoma arisaematis, Schisandra chinensis, the root of Chinese clematis, dark plum, centipede,
Chinese gall, cuttle bone, evodia rutaecarpa, clever rouge, radix fici simplicissimae, rhizoma cyperi, fennel seeds, asarum, longstamen onion bulb and the almonds, teasel root, radix scrophulariae, the flower bud of lily magnolia,
Hairyvein agrimony, pig label grass, Prunella vulgaris, paniculate swallowwort, inular flower, Common Leafflower Herb, rhizoma corydalis, semen coicis, radix polygonati officinalis, Herba Epimedii, Radix Polygalae, benefit are female
Grass, Radix Curcumae, cordate houttuynia, oriental wormwood, ginkgo leaf, tuber fleeceflower stem, purple perilla, rhizoma alismatis, cinnabar, Herba Lycopi, the dried immature fruit of citron orange, aster, rhizoma anemarrhenae, umbellate pore furgus, soap
Angle thorn, pearl.
Wherein, Radix Astragali, warm-natured, sweet in flavor, returns spleen, lung channel；With invigorating qi for strengthening superficies, pus draining and toxin expelling, diuresis, myogenic and other effects,
It is mainly used for deficiency of vital energy and acking in strength, rush down prolapse of the anus for a long time, spontaneous perspiration, oedema, uterine prolapse, chronic rephrilis protinuria, diabetes, the open sore part not to be cured long
The diseases such as conjunction.
Radix Angelicae Sinensis, warm-natured, sweet in flavor, pungent, return heart, liver, the spleen channel；With benefiting blood and regulating blood circulation, menstruction regulating and pain relieving, moisturizing dryness and lubricating intestine and other effects,
It is mainly used for controlling that irregular menstruation, menostasis abdominal pain, abdominal mass knot are poly-, metrorrhagia and metrostaxis, headache due to deficiency of blood, dizziness, flaccidity syndrome and arthralgia syndrome, intestines are dry and shit is difficult, after dysentery with bloody stool
Weight, ulcer sore steal, the diseases such as injury from falling down.
Uncaria, cool in nature, sweet in flavor, converge to heart and liver channels；With The flat liver of heat-clearing, relieve dizziness, high fever, infantile convulsions, epilepsy, etc. the effect of calming the frightened, and it is frightened to be mainly used for controlling children
The diseases such as Xian Chi Zong, adult's slight Hypertension, dizziness, dizzy, married woman's eclampsia.
SOD is present in the matrix of protokaryon and eukaryocyte, is a kind of removing O2-Key metal enzyme, to aerobe object
Defence oxygen toxicity plays critical effect.As domestic and international scientific research personnel deepens continuously to SOD research, the exploitation of SOD and
Using all by the concern of people, application range is related to the fields such as medicine, food, cosmetics all the time.
SOD has the effects of antitumor, anti-aging, anti-inflammatory.Existing research show SOD it is oral be effective, therefore SOD
It can be used as additive to be added directly into food；On the other hand, there is SOD preferable antioxidation to be known as the fresh-keeping of food
Agent can be added in food as antioxidant, the decline for preventing to cause food quality because of peroxidation,
The SOD (manganese) that we develop is in aubergine, and research has shown that there is characteristic resistant to high temperature, highly stable at 60 DEG C, and 80
After DEG C 2 hours of water-bath still with 70% vigor, after 2 hours of 90 DEG C of water-baths still with 30% vigor.
This product formula has from Yin-yang Syndrome selects three taste Chinese medicines in the medicinal material used, and has combined MnSOD resistant to high temperature,
To make this product have effects that yin-yang and mend, raising body energy.
Summary of the invention
It is an object of that present invention to provide the preparation method and applications of toxin-expelling and face nourishing, supplement energy food and medicine consangunity product.
Technical solution of the present invention includes:
In a first aspect, the present invention provides the preparation method of a kind of toxin-expelling and face nourishing, supplement energy food and medicine consangunity product,
It is characterized in being prepared from the following raw materials in parts by weight: 15 parts of Radix Astragali, 6 parts of Radix Angelicae Sinensis, 8 parts of uncaria, 1 part of SOD (manganese).
Wherein, the food and medicine consangunity product is that Chinese medicine is carried out to ultralow temperature crushing and SOD (manganese) is added, according to galenic pharmacy
Routine techniques is prepared.
Wherein, the detection of the Chinese medicine, the method is as follows:
(1) ultralow temperature crushing is carried out to Radix Astragali, Radix Angelicae Sinensis, uncaria respectively, obtains Ultramicro-powder；
(2) (1) Ultramicro-powder by described in is added in aqueous solution respectively, after extracting 30min, through centrifugation, 0.45 μm of miillpore filter
Filtering, obtains sample；
(3) (2) sample by described in carries out efficient liquid phase chromatographic analysis respectively.
Wherein, the preparation method of the food and medicine consangunity product, which is characterized in that formula is as follows:
Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
Wherein, the preparation method of the food and medicine consangunity product, which is characterized in that the SOD (manganese), is by pet28a-
Mnsod imports Escherichia coli (E.coli) expression and forms recombinant bacterium to carry out fermented and cultured, can finally obtain by ultrasonication
To SOD (manganese).
Second aspect, the present invention provide a kind of toxin-expelling and face nourishing, supplement energy food and medicine consangunity product comprising said medicine
Any proportion combination and pharmaceutically acceptable carrier and auxiliary material.
Wherein, the food and medicine consangunity product, dosage form include: tablet, capsule, pulvis, powder, pill, granule and
Other available dosage forms.
The third aspect, the new application that the present invention provides a kind of toxin-expelling and face nourishing, supplements energy food and medicine consangunity product, it may be assumed that one
Application of the preparation of kind aforementioned pharmaceutical compositions for toxin-expelling and face nourishing, supplement energy food and medicine consangunity product.
Compared with prior art, beneficial effects of the present invention are as follows:
Pharmaceutical composition provided by the invention, including Radix Astragali, Radix Angelicae Sinensis, uncaria, the pharmaceutical composition are playing enhancing body
Under the premise of immune function, anti-aging, benefiting blood and regulating blood circulation, decompression, in conjunction with anti-oxidant, anti-aging the special efficacy of SOD (manganese),
Play better effect in terms of toxin-expelling and face nourishing, supplement energy, can further be made food, health care product and other and improve health
Relevant food and medicine consangunity product, has broad application prospects.
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, with presently preferred embodiments of the present invention and attached drawing is cooperated to be described in detail below.
Detailed description of the invention
Fig. 1 is toxin-expelling and face nourishing, the preparation flow figure for supplementing energy food and medicine consangunity product；
Fig. 2 be Radix Astragali high performance liquid chromatography spectrogram (abscissa be ultraviolet light absorption angle value, ordinate is retention time.)；
Fig. 3 be Radix Angelicae Sinensis high performance liquid chromatography spectrogram (abscissa be ultraviolet light absorption angle value, ordinate is retention time.)；
Fig. 4 be uncaria high performance liquid chromatography spectrogram (abscissa be ultraviolet light absorption angle value, ordinate is retention time.)；
Fig. 5 is SOD (manganese) tomograph.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment one: Radix Astragali, Radix Angelicae Sinensis, uncaria quality testing
1, the quality inspection steps of Radix Astragali are as follows:
(1) ultralow temperature crushing is carried out to Radix Astragali with the pulverizer of 300-600 mesh screen, crushing temperature is -30 DEG C, is surpassed
Micro mist；
(2) it weighs 90g Radix Astragali Ultramicro-powder to be added in 540mL aqueous solution, every 10min stirring is primary, stirs 1min every time, soaks
After mentioning 30min, leaching liquor is centrifuged 10min, takes supernatant to cross 0.45 μm of miillpore filter, obtain sample in 10 DEG C；
(3) sample obtained by step (2) is subjected to HPLC analysis:
(a) astragalus extraction Supernatant samples obtained in step (2) are taken, are analyzed for HPLC, mobile phase is methanol, water；It washes
Off-square formula are as follows: 5% methanol elutes 5min, and 5%-100% methanol elutes 30min, and 100% methanol elutes 5min；Applied sample amount: 20 μ
L；Ultraviolet detection wavelength: 260nm；Its high performance liquid chromatography spectrogram is as shown in Figure 2.
2, for Radix Angelicae Sinensis quality inspection steps referring to above-mentioned quality inspection steps, high performance liquid chromatography spectrogram is as shown in Figure 3.
3, for uncaria quality inspection steps referring to above-mentioned quality inspection steps, high performance liquid chromatography spectrogram figure is as shown in Figure 4.
The preparation and determination of activity of embodiment two: SOD (manganese)
1, the ingredient of LB culture medium
Sodium chloride (5g/L), peptone (10g/L), yeast powder (5g/L).
2, first order seed activates
The recombination of the kanamycins and 10L of 150L is successively added into the LB culture medium for bacterium of having gone out under superclean bench
Bacterium cultivates 16.5h under the conditions of 37 DEG C, 200rpm/min.
3, the ingredient of supplemented medium
Peptone (60g/L), yeast powder (30g/L)
4, the ingredient of fermentation medium
Disodium hydrogen phosphate (8g/L), potassium dihydrogen phosphate (4g/L), magnesium chloride (0.3g/L), manganese chloride (0.1g/L), GPE:
1mL/L, glycerol (5g/L), sodium chloride (5g/L), peptone (10g/L), yeast powder (5g/L)
5, secondary seed activates
The first order seed of certain volume is taken to be added in the LB culture medium of activation secondary seed, in 37 DEG C, 200rpm/min item
4.5h is cultivated under part.
6, it ferments
Secondary seed is accessed in fermentor and carries out fermentation 4h, carries out cooling induction, is cooled to 25 DEG C, is added final concentration of
The IPTG of 0.08mmol/L, induction time 4h.
7, fermentation liquid is collected, in 3500rpm/min, 30min is centrifuged, collects thallus, it is broken to carry out ultrasound to the thallus of collection
Broken, finally freeze-drying obtains SOD (manganese).
8, according to the step of CuZn/Mn-SOD activity detection kit (the green skies, S0103, WST-8 method) to SOD (manganese)
Activity is detected.
Embodiment three: tablet
Formula: Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
Production method: Radix Astragali Ultramicro-powder, Angelica micropowder, uncaria Ultramicro-powder, SOD (manganese) are sufficiently mixed with auxiliary material, pressure
Piece both obtains tablet.
Example IV: capsule
Formula: Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
Production method: Radix Astragali Ultramicro-powder, Angelica micropowder, uncaria Ultramicro-powder, SOD (manganese) are mixed well with auxiliary material and is made
Medicinal subdivision, medicinal subdivision carried out with capsule filler it is filling, both capsule.
Embodiment five: pulvis
Formula: Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
Production method: Radix Astragali Ultramicro-powder, Angelica micropowder, uncaria Ultramicro-powder, SOD (manganese) are mixed well and is added with auxiliary material
Add stabilizer appropriate to be mixed together crushing, both obtains pulvis.
Embodiment six: powder
Formula: Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
Production method: Radix Astragali Ultramicro-powder, Angelica micropowder, uncaria Ultramicro-powder, SOD (manganese) are mixed well with auxiliary material, both
Obtain powder.
Embodiment seven: pill
Formula: Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
Production method: by ball made of Radix Astragali Ultramicro-powder, Angelica micropowder, uncaria Ultramicro-powder, SOD (manganese) and bonding auxiliary material
Shape or class ball slice preparation both obtain pill.
Embodiment eight: granule
Formula: Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
Production method: Radix Astragali Ultramicro-powder, Angelica micropowder, uncaria Ultramicro-powder, SOD (manganese) are mixed well with auxiliary material, system
The standby dry granular formulation with certain particle size, both obtains granule.
Claims (8)
1. a kind of toxin-expelling and face nourishing, the preparation method for supplementing energy food and medicine consangunity product, it is characterized in that by following weight proportion
Made of raw material: 15 parts of Radix Astragali, 6 parts of Radix Angelicae Sinensis, 8 parts of uncaria, 1 part of SOD (manganese).
2. the preparation method of food and medicine consangunity product according to claim 1, which is characterized in that the food and medicine consangunity product is
Chinese medicine is subjected to ultralow temperature crushing and is added SOD (manganese), is prepared according to galenic pharmacy routine techniques.
3. the preparation method of food and medicine consangunity product according to claim 1, which is characterized in that the Chinese medicine quality testing,
Method is as follows:
(1) ultralow temperature crushing is carried out to Radix Astragali, Radix Angelicae Sinensis, uncaria respectively, obtains Ultramicro-powder；
(2) (1) Ultramicro-powder by described in is added in aqueous solution respectively, after extracting 30min, is centrifuged, 0.45 μm of filtering with microporous membrane,
Obtain sample；
(3) (2) sample by described in carries out efficient liquid phase chromatographic analysis respectively.
4. the preparation method of food and medicine consangunity product according to claim 1, which is characterized in that formula is as follows:
Radix Astragali 2400g, Radix Angelicae Sinensis 960g, uncaria 1280g, SOD (manganese) 20g.
5. the preparation method of food and medicine consangunity product according to claim 1, which is characterized in that the SOD (manganese), be by
Pet28a-Mnsod imports Escherichia coli (E.coli) expression and forms recombinant bacterium to carry out fermented and cultured, finally by ultrasonic broken
It is broken that SOD (manganese) can be obtained.
6. the preparation method of food and medicine consangunity product according to claim 1, which is characterized in that it includes that claim 1-6 appoints
One drug any proportion combination and pharmaceutically acceptable carrier and auxiliary material.
7. the preparation method of food and medicine consangunity product according to claim 1, which is characterized in that the food and medicine consangunity product,
Dosage form includes: tablet, capsule, pulvis, powder, pill, granule and other available dosage forms.
8. a kind of if any one of claim 1-7 described pharmaceutical composition is for toxin-expelling and face nourishing, the supplement homologous production of energy food
The preparation method and application of product.
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| CN114624357A (en) * | 2022-03-10 | 2022-06-14 | 中国中医科学院中药研究所 | Chinese medicine compound preparation characteristic atlas and its establishing method |
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| CN112107649A (en) * | 2020-11-03 | 2020-12-22 | 浙江省中医院、浙江中医药大学附属第一医院（浙江省东方医院） | Traditional Chinese medicine composition for treating oral mucosa injury caused by antitumor drugs |
| CN112494593A (en) * | 2020-12-31 | 2021-03-16 | 广东高山动物药业有限公司 | Pure traditional Chinese medicine preparation for resisting respiratory syndrome for poultry |
| CN114624357A (en) * | 2022-03-10 | 2022-06-14 | 中国中医科学院中药研究所 | Chinese medicine compound preparation characteristic atlas and its establishing method |
| CN114624357B (en) * | 2022-03-10 | 2023-04-18 | 中国中医科学院中药研究所 | Chinese medicine compound preparation characteristic atlas and its establishing method |
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