CN113293139B - 重组蛋白的高效选择性 - Google Patents

重组蛋白的高效选择性 Download PDF

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Publication number
CN113293139B
CN113293139B CN202110402963.4A CN202110402963A CN113293139B CN 113293139 B CN113293139 B CN 113293139B CN 202110402963 A CN202110402963 A CN 202110402963A CN 113293139 B CN113293139 B CN 113293139B
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China
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leu
gene
interest
cell
phe
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Chinese (zh)
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CN113293139A (zh
Inventor
D·德什潘德
D·布拉科夫
陈刚
J·凡德尔
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202110402963.4A 2014-08-19 2015-08-19 重组蛋白的高效选择性 Active CN113293139B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462039416P 2014-08-19 2014-08-19
US62/039,416 2014-08-19
PCT/US2015/045793 WO2016028838A1 (en) 2014-08-19 2015-08-19 Efficient selectivity of recombinant proteins
CN201580044100.7A CN107075549B (zh) 2014-08-19 2015-08-19 重组蛋白的高效选择性

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201580044100.7A Division CN107075549B (zh) 2014-08-19 2015-08-19 重组蛋白的高效选择性

Publications (2)

Publication Number Publication Date
CN113293139A CN113293139A (zh) 2021-08-24
CN113293139B true CN113293139B (zh) 2025-04-29

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CN202110402963.4A Active CN113293139B (zh) 2014-08-19 2015-08-19 重组蛋白的高效选择性
CN201580044100.7A Active CN107075549B (zh) 2014-08-19 2015-08-19 重组蛋白的高效选择性

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EP (2) EP3183359B1 (enExample)
JP (4) JP7115851B2 (enExample)
KR (2) KR102556383B1 (enExample)
CN (2) CN113293139B (enExample)
AR (1) AR102333A1 (enExample)
AU (3) AU2015305565B9 (enExample)
CA (1) CA2957036A1 (enExample)
CY (1) CY1124079T1 (enExample)
DK (1) DK3183359T3 (enExample)
EA (1) EA035340B1 (enExample)
ES (1) ES2849728T3 (enExample)
HR (1) HRP20210301T1 (enExample)
HU (1) HUE054069T2 (enExample)
IL (3) IL287020B (enExample)
LT (1) LT3183359T (enExample)
MA (1) MA40371B1 (enExample)
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SG (2) SG11201701121UA (enExample)
SI (1) SI3183359T1 (enExample)
SM (1) SMT202100054T1 (enExample)
TW (3) TWI832364B (enExample)
WO (1) WO2016028838A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
WO2019157395A1 (en) 2018-02-08 2019-08-15 Applied Stemcells, Inc Methods for screening variant of target gene
CN114585597A (zh) 2019-10-23 2022-06-03 佳能株式会社 制造陶瓷制品的方法和陶瓷制品

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066919A2 (en) * 2007-11-23 2009-05-28 Korea Research Institute Of Bioscience And Biotechnology Gpt gene as a marker for selecting plant transformant
CN102307993A (zh) * 2008-12-09 2012-01-04 哈洛齐梅公司 延长的可溶性ph20多肽及其用途

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US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US6077835A (en) 1994-03-23 2000-06-20 Case Western Reserve University Delivery of compacted nucleic acid to cells
KR970029803A (ko) 1995-11-03 1997-06-26 김광호 반도체 메모리장치의 프리차지 회로
US5972650A (en) 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
US6281005B1 (en) 1999-05-14 2001-08-28 Copernicus Therapeutics, Inc. Automated nucleic acid compaction device
WO2001053312A1 (en) * 1999-12-23 2001-07-26 Hyseq, Inc. Novel nucleic acids and polypeptides
EP1392859B1 (en) 2001-01-16 2006-05-10 Regeneron Pharmaceuticals, Inc. Isolating cells expressing secreted proteins
US7192737B2 (en) 2002-03-29 2007-03-20 Xoma Technology Ltd. Methods and materials for increasing expression of recombinant polypeptides
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
US7384744B2 (en) * 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
JP2005027599A (ja) 2003-07-09 2005-02-03 Nara Institute Of Science & Technology 植物由来遺伝子を用いた遺伝子導入植物の選抜法
EP1586585A1 (de) * 2004-04-14 2005-10-19 F. Hoffmann-La Roche Ag Expressionssystem zur Herstellung von IL-15/Fc-Fusionsproteinen und ihre Verwendung
AR064200A1 (es) 2006-12-07 2009-03-18 Dow Agrosciences Llc Genes marcadores seleccionables
ES2522615T3 (es) 2007-06-04 2014-11-17 Regeneron Pharmaceuticals, Inc. Regiones de expresión y estabilidad potenciadas
ES2570853T3 (es) * 2007-12-07 2016-05-20 Zymogenetics Inc Moléculas de anticuerpo humanizadas específicas para IL-31
US20110091495A1 (en) * 2008-04-22 2011-04-21 Rutgers, The State University Of New Jersey Hcv e2 construct compositions and methods
EP2674495A1 (en) 2012-06-14 2013-12-18 Sanofi CHO expression system
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066919A2 (en) * 2007-11-23 2009-05-28 Korea Research Institute Of Bioscience And Biotechnology Gpt gene as a marker for selecting plant transformant
CN102307993A (zh) * 2008-12-09 2012-01-04 哈洛齐梅公司 延长的可溶性ph20多肽及其用途

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Publication number Publication date
HRP20210301T1 (hr) 2021-04-02
AU2015305565B9 (en) 2020-05-21
TW202124716A (zh) 2021-07-01
KR20220080215A (ko) 2022-06-14
MA40371B1 (fr) 2021-03-31
JP7115851B2 (ja) 2022-08-09
SMT202100054T1 (it) 2021-03-15
AU2023200752A1 (en) 2023-03-09
BR112017003074A2 (pt) 2018-03-06
MY191745A (en) 2022-07-13
DK3183359T3 (da) 2021-02-01
EP3901274A1 (en) 2021-10-27
MA40371A (fr) 2017-06-28
AU2023200752B2 (en) 2024-02-29
IL287020B (en) 2022-09-01
CN107075549A (zh) 2017-08-18
TW201615833A (zh) 2016-05-01
TWI777374B (zh) 2022-09-11
US10457959B2 (en) 2019-10-29
CA2957036A1 (en) 2016-02-25
IL295361B1 (en) 2023-04-01
EA201790241A1 (ru) 2017-06-30
IL250531A0 (en) 2017-03-30
IL287020A (en) 2021-12-01
US20200063159A1 (en) 2020-02-27
CN113293139A (zh) 2021-08-24
IL295361A (en) 2022-10-01
AU2020205336A1 (en) 2020-08-06
IL250531B (en) 2021-10-31
JP2017525368A (ja) 2017-09-07
US9732357B2 (en) 2017-08-15
SI3183359T1 (sl) 2021-03-31
AU2020205336B2 (en) 2022-11-10
SG11201701121UA (en) 2017-03-30
US20160053280A1 (en) 2016-02-25
MX2017002203A (es) 2017-05-22
JP2024166420A (ja) 2024-11-28
US20210340564A1 (en) 2021-11-04
IL295361B2 (en) 2023-08-01
ES2849728T3 (es) 2021-08-20
EP3183359A4 (en) 2018-04-04
CN107075549B (zh) 2021-04-20
JP7260510B2 (ja) 2023-04-18
JP2023076667A (ja) 2023-06-01
TWI832364B (zh) 2024-02-11
LT3183359T (lt) 2021-02-25
PL3183359T3 (pl) 2021-08-02
JP7561232B2 (ja) 2024-10-03
KR102406976B1 (ko) 2022-06-10
MX384990B (es) 2025-03-14
EP3183359B1 (en) 2020-12-16
AU2015305565B2 (en) 2020-04-30
JP2020174681A (ja) 2020-10-29
MX2021009154A (es) 2021-09-10
PT3183359T (pt) 2021-02-03
EP3183359A1 (en) 2017-06-28
SG10201707859RA (en) 2017-10-30
US20170335341A1 (en) 2017-11-23
CY1124079T1 (el) 2022-05-27
RS61371B1 (sr) 2021-02-26
EA035340B1 (ru) 2020-05-29
AR102333A1 (es) 2017-02-22
KR102556383B1 (ko) 2023-07-17
KR20170055481A (ko) 2017-05-19
US11085053B2 (en) 2021-08-10
TWI719945B (zh) 2021-03-01
WO2016028838A1 (en) 2016-02-25
TW202246506A (zh) 2022-12-01
HUE054069T2 (hu) 2021-08-30
AU2015305565A1 (en) 2017-03-09

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