CN112626128B - 细胞转染方法 - Google Patents

细胞转染方法 Download PDF

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Publication number
CN112626128B
CN112626128B CN202011320159.3A CN202011320159A CN112626128B CN 112626128 B CN112626128 B CN 112626128B CN 202011320159 A CN202011320159 A CN 202011320159A CN 112626128 B CN112626128 B CN 112626128B
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avian
embryo
transfection
transfection reagent
germ cells
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CN112626128A (zh
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S·G·蒂亚克
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Commonwealth Scientific and Industrial Research Organization CSIRO
Aviagen Inc
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Commonwealth Scientific and Industrial Research Organization CSIRO
Aviagen Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
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    • C12N2800/90—Vectors containing a transposable element

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CN202011320159.3A 2012-04-20 2013-04-19 细胞转染方法 Active CN112626128B (zh)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261636331P 2012-04-20 2012-04-20
US61/636,331 2012-04-20
US201361783823P 2013-03-14 2013-03-14
US61/783,823 2013-03-14
AU2013204327A AU2013204327B2 (en) 2012-04-20 2013-04-12 Cell transfection method
AU2013204327 2013-04-12
CN201380031734.XA CN104619848B (zh) 2012-04-20 2013-04-19 细胞转染方法
PCT/AU2013/000414 WO2013155572A1 (en) 2012-04-20 2013-04-19 Cell transfection method

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CN112626128B true CN112626128B (zh) 2025-03-28

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EP (2) EP2839016B1 (cg-RX-API-DMAC7.html)
JP (1) JP6531239B2 (cg-RX-API-DMAC7.html)
KR (1) KR102076784B1 (cg-RX-API-DMAC7.html)
CN (2) CN112626128B (cg-RX-API-DMAC7.html)
AU (2) AU2013204327B2 (cg-RX-API-DMAC7.html)
BR (1) BR112014026186B8 (cg-RX-API-DMAC7.html)
ES (2) ES2704155T3 (cg-RX-API-DMAC7.html)
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