ATE312167T1 - Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung - Google Patents

Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung

Info

Publication number
ATE312167T1
ATE312167T1 AT98939102T AT98939102T ATE312167T1 AT E312167 T1 ATE312167 T1 AT E312167T1 AT 98939102 T AT98939102 T AT 98939102T AT 98939102 T AT98939102 T AT 98939102T AT E312167 T1 ATE312167 T1 AT E312167T1
Authority
AT
Austria
Prior art keywords
pgcs
production
cloning
chimerization
cell lines
Prior art date
Application number
AT98939102T
Other languages
English (en)
Inventor
De Leon F Ponce
Eckles Ave Hall
James M Robl
Steven L Stice
D Joseph Jerry
Original Assignee
Univ Massachusetts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Massachusetts filed Critical Univ Massachusetts
Application granted granted Critical
Publication of ATE312167T1 publication Critical patent/ATE312167T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT98939102T 1997-08-04 1998-08-04 Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung ATE312167T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5467797P 1997-08-04 1997-08-04
PCT/US1998/015636 WO1999006534A1 (en) 1997-08-04 1998-08-04 Production of avian embryonic germ (eg) cell lines by prolonged culturing of pgcs, use thereof for cloning and chimerization

Publications (1)

Publication Number Publication Date
ATE312167T1 true ATE312167T1 (de) 2005-12-15

Family

ID=21992766

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98939102T ATE312167T1 (de) 1997-08-04 1998-08-04 Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung

Country Status (12)

Country Link
EP (2) EP1019491B1 (de)
JP (1) JP2003517261A (de)
CN (1) CN1273599A (de)
AT (1) ATE312167T1 (de)
AU (1) AU738357B2 (de)
BR (1) BR9811832A (de)
CA (1) CA2300336A1 (de)
DE (1) DE69832716T2 (de)
ES (1) ES2255174T3 (de)
IL (1) IL134366A (de)
NZ (1) NZ502713A (de)
WO (1) WO1999006534A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100378747B1 (ko) * 1999-02-11 2003-04-07 한미약품공업 주식회사 조류의 다능성 배자생식세포주 및 이의 제조방법
DE60037107T2 (de) * 1999-02-11 2008-09-25 Hanmi Pharm. Co., Ltd. Pluripotente embryonale keimzellinie aus vögeln
US6333192B1 (en) 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
AU7491800A (en) * 1999-09-15 2001-04-17 Therapeutic Human Polyclonals, Inc. Immunotherapy with substantially human polyclonal antibody preparations purifiedfrom genetically engineered birds
JP3580221B2 (ja) 2000-04-12 2004-10-20 ヤマハ株式会社 電気弦楽器
IL154751A0 (en) 2000-08-03 2003-10-31 Humanized antibodies, recombination vectors, transgenic vectors and methods for the preparation of humanized antibodies from transgenic animals
BR0211895A (pt) 2001-08-13 2006-04-04 Embrex Inc métodos de injetar um ovo avìcola, injetar uma pluralidade de ovos avìculas, de produzir uma ave quimérica e uma ave transgênica
WO2003024199A2 (en) * 2001-09-21 2003-03-27 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
FR2836924B1 (fr) * 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
KR100502889B1 (ko) 2003-04-21 2005-07-20 한미약품(주) 조류 원시생식세포의 생식선 전이 효율의 개선방법
US20060174362A1 (en) 2005-02-01 2006-08-03 Origen Therapeutics, Inc. Long-term culture of avian primordial germ cells (PGCs)
CN100366734C (zh) * 2006-03-02 2008-02-06 扬州大学 一种鸡精原细胞分离纯化方法
BRPI0714257A8 (pt) 2006-08-09 2017-05-09 Valneva "métodos para a cultura de células tronco embriónarias (es) de aves, para modificar geneticamente as células tronco embrionárias, para a obtenção de um pintinho quimérico, para a obtenção de um progênie do pintinho, para a analise do polimorfismo genético, meio de cultura para células tronco embrionárias de aves e meio de cultura".
ATE536374T1 (de) 2006-09-01 2011-12-15 Therapeutic Human Polyclonals Inc Erhöhte expression von humanem oder humanisiertem immunglobulin bei nicht-humanen transgenen tieren
EP1985305A1 (de) 2007-04-24 2008-10-29 Vivalis Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren
AU2013204327B2 (en) 2012-04-20 2016-09-01 Aviagen Cell transfection method
CN103674653B (zh) * 2013-12-19 2016-08-24 浙江省农业科学院 一种原始生殖细胞的爬片方法
CZ2016348A3 (cs) * 2016-06-10 2018-01-10 Biopharm, Výzkumný Ústav Biofarmacie A Veterinárních Léčiv, A.S. Způsob produkce spermií a transgenních ptáků
CN108384749B (zh) * 2017-12-07 2021-08-17 广西大学 鸡性腺原始生殖细胞快速分离与建系的方法
CN113355359A (zh) * 2021-06-11 2021-09-07 扬州大学 一种高效生产类克隆鸡的方法
CN116286612B (zh) * 2023-03-30 2023-10-20 广东省农业科学院动物科学研究所 以mTOR、SMAD信号通路和p53基因为靶点在提高鸡PGCs体外建系效率中的应用
CN117586944B (zh) * 2024-01-19 2024-04-30 成都思澍生物科技有限公司 一种鸡原始生殖细胞体外无血清扩增培养的方法
CN119192326B (zh) * 2024-10-23 2025-06-17 四川大学 重组cFGF2蛋白和鸟类原始生殖细胞培养基及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US6156569A (en) * 1997-08-04 2000-12-05 University Of Massachusetts Office Of Vice Chancellor For Research At Amherst Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians

Also Published As

Publication number Publication date
BR9811832A (pt) 2000-08-15
ES2255174T3 (es) 2006-06-16
EP1019491A4 (de) 2001-06-06
CN1273599A (zh) 2000-11-15
DE69832716T2 (de) 2006-09-14
EP1019491A1 (de) 2000-07-19
EP1650294A1 (de) 2006-04-26
AU738357B2 (en) 2001-09-13
JP2003517261A (ja) 2003-05-27
EP1019491B1 (de) 2005-12-07
CA2300336A1 (en) 1999-02-11
AU8759498A (en) 1999-02-22
IL134366A (en) 2007-12-03
WO1999006534A1 (en) 1999-02-11
NZ502713A (en) 2003-09-26
DE69832716D1 (de) 2006-01-12

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