DE69832716D1 - Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung - Google Patents

Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung

Info

Publication number
DE69832716D1
DE69832716D1 DE69832716T DE69832716T DE69832716D1 DE 69832716 D1 DE69832716 D1 DE 69832716D1 DE 69832716 T DE69832716 T DE 69832716T DE 69832716 T DE69832716 T DE 69832716T DE 69832716 D1 DE69832716 D1 DE 69832716D1
Authority
DE
Germany
Prior art keywords
pgcs
cloning
chimerizing
preparation
cell lines
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69832716T
Other languages
English (en)
Other versions
DE69832716T2 (de
Inventor
De Leon Abel Ponce
M Robl
L Stice
Joseph Jerry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Massachusetts UMass
Original Assignee
University of Massachusetts UMass
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Massachusetts UMass filed Critical University of Massachusetts UMass
Application granted granted Critical
Publication of DE69832716D1 publication Critical patent/DE69832716D1/de
Publication of DE69832716T2 publication Critical patent/DE69832716T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0271—Chimeric animals, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69832716T 1997-08-04 1998-08-04 Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung Expired - Fee Related DE69832716T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5467797P 1997-08-04 1997-08-04
US54677P 1997-08-04
PCT/US1998/015636 WO1999006534A1 (en) 1997-08-04 1998-08-04 Production of avian embryonic germ (eg) cell lines by prolonged culturing of pgcs, use thereof for cloning and chimerization

Publications (2)

Publication Number Publication Date
DE69832716D1 true DE69832716D1 (de) 2006-01-12
DE69832716T2 DE69832716T2 (de) 2006-09-14

Family

ID=21992766

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69832716T Expired - Fee Related DE69832716T2 (de) 1997-08-04 1998-08-04 Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung

Country Status (12)

Country Link
EP (2) EP1650294A1 (de)
JP (1) JP2003517261A (de)
CN (1) CN1273599A (de)
AT (1) ATE312167T1 (de)
AU (1) AU738357B2 (de)
BR (1) BR9811832A (de)
CA (1) CA2300336A1 (de)
DE (1) DE69832716T2 (de)
ES (1) ES2255174T3 (de)
IL (1) IL134366A (de)
NZ (1) NZ502713A (de)
WO (1) WO1999006534A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100378747B1 (ko) * 1999-02-11 2003-04-07 한미약품공업 주식회사 조류의 다능성 배자생식세포주 및 이의 제조방법
EP1155114B1 (de) * 1999-02-11 2007-11-14 Hanmi Pharm. Co., Ltd. Pluripotente embryonale keimzellinie aus vögeln
US6333192B1 (en) 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
WO2001019394A2 (en) * 1999-09-15 2001-03-22 Therapeutic Human Polyclonals, Inc. Immunotherapy with substantially human polyclonal antibody preparations purified from genetically engineered birds
JP3580221B2 (ja) 2000-04-12 2004-10-20 ヤマハ株式会社 電気弦楽器
CA2634294A1 (en) 2000-08-03 2002-02-14 Therapeutic Human Polyclonals, Inc. Production of humanized antibodies in transgenic animals
WO2003025159A1 (en) 2001-08-13 2003-03-27 Embrex, Inc. Methods for injecting avian eggs
AU2002341795A1 (en) * 2001-09-21 2003-04-01 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
FR2836924B1 (fr) * 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
KR100502889B1 (ko) 2003-04-21 2005-07-20 한미약품(주) 조류 원시생식세포의 생식선 전이 효율의 개선방법
US20060174362A1 (en) * 2005-02-01 2006-08-03 Origen Therapeutics, Inc. Long-term culture of avian primordial germ cells (PGCs)
CN100366734C (zh) * 2006-03-02 2008-02-06 扬州大学 一种鸡精原细胞分离纯化方法
RU2473688C2 (ru) 2006-08-09 2013-01-27 Вивалис Способ получения трансгенных птиц с использованием эмбриональных стволовых клеток
EP2064325B1 (de) 2006-09-01 2011-12-07 Therapeutic Human Polyclonals, Inc. Erhöhte expression von humanem oder humanisiertem immunglobulin bei nicht-humanen transgenen tieren
EP1985305A1 (de) 2007-04-24 2008-10-29 Vivalis Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren
AU2013204327B2 (en) 2012-04-20 2016-09-01 Aviagen Cell transfection method
CN103674653B (zh) * 2013-12-19 2016-08-24 浙江省农业科学院 一种原始生殖细胞的爬片方法
CZ307102B6 (cs) * 2016-06-10 2018-01-10 Biopharm, Výzkumný Ústav Biofarmacie A Veterinárních Léčiv, A.S. Způsob produkce spermií a transgenních ptáků
CN108384749B (zh) * 2017-12-07 2021-08-17 广西大学 鸡性腺原始生殖细胞快速分离与建系的方法
CN113355359A (zh) * 2021-06-11 2021-09-07 扬州大学 一种高效生产类克隆鸡的方法
CN116286612B (zh) * 2023-03-30 2023-10-20 广东省农业科学院动物科学研究所 以mTOR、SMAD信号通路和p53基因为靶点在提高鸡PGCs体外建系效率中的应用
CN117586944B (zh) * 2024-01-19 2024-04-30 成都思澍生物科技有限公司 一种鸡原始生殖细胞体外无血清扩增培养的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US6156569A (en) * 1997-08-04 2000-12-05 University Of Massachusetts Office Of Vice Chancellor For Research At Amherst Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians

Also Published As

Publication number Publication date
JP2003517261A (ja) 2003-05-27
EP1019491A4 (de) 2001-06-06
DE69832716T2 (de) 2006-09-14
NZ502713A (en) 2003-09-26
AU738357B2 (en) 2001-09-13
EP1019491B1 (de) 2005-12-07
EP1019491A1 (de) 2000-07-19
CA2300336A1 (en) 1999-02-11
WO1999006534A1 (en) 1999-02-11
ES2255174T3 (es) 2006-06-16
AU8759498A (en) 1999-02-22
EP1650294A1 (de) 2006-04-26
CN1273599A (zh) 2000-11-15
BR9811832A (pt) 2000-08-15
IL134366A (en) 2007-12-03
ATE312167T1 (de) 2005-12-15

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: UNIVERSITY OF MASSACHUSETTS INSTITUTION OF HIGHER

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee