CN112469821A - 一种制备重组人凝血因子ⅷ的方法 - Google Patents

一种制备重组人凝血因子ⅷ的方法 Download PDF

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Publication number
CN112469821A
CN112469821A CN201980049311.8A CN201980049311A CN112469821A CN 112469821 A CN112469821 A CN 112469821A CN 201980049311 A CN201980049311 A CN 201980049311A CN 112469821 A CN112469821 A CN 112469821A
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factor viii
recombinant human
culture
cell
blood coagulation
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CN201980049311.8A
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CN112469821B (zh
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胡伟伟
尚战强
杜敏
陆建胜
苏贤德
张哲文
谢一龙
费如翔
吴鼎
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Chia Tai Tianqing Pharmaceutical Group Co Ltd
Nanjing Shunxin Pharmaceutical Co Ltd
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Chia Tai Tianqing Pharmaceutical Group Co Ltd
Nanjing Shunxin Pharmaceutical Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

一种制备重组人凝血因子Ⅷ的方法，包括利用波浪式生物反应器培养表达重组人凝血因子Ⅷ的人胚肾细胞293/N27‑7以及从细胞培养液中分离并纯化重组人凝血Ⅷ因子。波浪生物反应器混合效率高，气液交换充分，泡沫少且剪切力低，避免了搅拌式不锈钢反应器浆叶端和气泡对细胞的伤害。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201980049311.8A 2018-07-26 2019-07-26 一种制备重组人凝血因子ⅷ的方法 Active CN112469821B (zh)

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CN2018108596181 2018-07-26
CN201810859618 2018-07-26
PCT/CN2019/097984 WO2020020364A1 (zh) 2018-07-26 2019-07-26 一种制备重组人凝血因子ⅷ的方法

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PH (1) PH12021550195A1 (zh)
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ZA (1) ZA202100861B (zh)

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CN115819608A (zh) * 2022-10-08 2023-03-21 盛禾(中国)生物制药有限公司 一种纯化融合蛋白的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1454257A (zh) * 2000-03-22 2003-11-05 奥克塔金尼有限责任公司 重组凝血因子在人类细胞系中的生产
CN103215308A (zh) * 2013-02-01 2013-07-24 中国科学院苏州生物医学院工程技术研究所 表达重组人fviii的整合质粒、细胞株及其构建方法和应用
CN103517919A (zh) * 2011-05-13 2014-01-15 欧克塔医药公司 在重组fviii的生产中提高真核细胞生产率的方法
CN107287265A (zh) * 2016-03-31 2017-10-24 正大天晴药业集团南京顺欣制药有限公司 一种制备重组人凝血因子ⅷ的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102311495A (zh) * 2010-06-30 2012-01-11 上海同科生物科技有限公司 新型重组人凝血因子ⅷ及其生产方法
CN102321668A (zh) * 2011-07-06 2012-01-18 中国人民解放军军事医学科学院野战输血研究所 一种表达重组人凝血因子ⅶ的方法及其专用载体
CN103555759A (zh) * 2013-07-05 2014-02-05 兰诺生物技术无锡有限公司 利用家兔乳腺生物反应器制备第八凝血因子的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1454257A (zh) * 2000-03-22 2003-11-05 奥克塔金尼有限责任公司 重组凝血因子在人类细胞系中的生产
CN103517919A (zh) * 2011-05-13 2014-01-15 欧克塔医药公司 在重组fviii的生产中提高真核细胞生产率的方法
CN103215308A (zh) * 2013-02-01 2013-07-24 中国科学院苏州生物医学院工程技术研究所 表达重组人fviii的整合质粒、细胞株及其构建方法和应用
CN107287265A (zh) * 2016-03-31 2017-10-24 正大天晴药业集团南京顺欣制药有限公司 一种制备重组人凝血因子ⅷ的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
STEFAN WINGE ET AL.: "Development, upscaling and validation of the purification process for human-cl rhFVIII (Nuwiq), a new generation recombinant factor VIII produced in a human cell-line", 《PROTEIN EXPRESSION AND PURIFICATION》 *
董艳荣 等: "重组凝血八因子及相关药物研究进展", 《中国新药杂志》 *

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ZA202100861B (en) 2022-05-25
WO2020020364A1 (zh) 2020-01-30
CN112469821B (zh) 2021-12-14
PH12021550195A1 (en) 2022-02-21

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