CN112424333A - 用于在生物反应器中进行细胞培养的系统 - Google Patents

用于在生物反应器中进行细胞培养的系统 Download PDF

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Publication number
CN112424333A
CN112424333A CN201980034566.7A CN201980034566A CN112424333A CN 112424333 A CN112424333 A CN 112424333A CN 201980034566 A CN201980034566 A CN 201980034566A CN 112424333 A CN112424333 A CN 112424333A
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cells
bioreactor
micro
cell
compartment
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Chinese (zh)
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马克西姆·法耶尤科斯
凯文·亚历山德里
皮埃尔·纳索伊
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Institute Of Theory And Applied Optics
Centre National de la Recherche Scientifique CNRS
Universite de Bordeaux
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Institute Of Theory And Applied Optics
Centre National de la Recherche Scientifique CNRS
Universite de Bordeaux
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
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  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
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  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980034566.7A 2018-05-21 2019-05-20 用于在生物反应器中进行细胞培养的系统 Pending CN112424333A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1854207 2018-05-21
FR1854207A FR3081168B1 (fr) 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur
PCT/FR2019/051144 WO2019224467A1 (fr) 2018-05-21 2019-05-20 Système de culture cellulaire en bioréacteur

Publications (1)

Publication Number Publication Date
CN112424333A true CN112424333A (zh) 2021-02-26

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CN201980034566.7A Pending CN112424333A (zh) 2018-05-21 2019-05-20 用于在生物反应器中进行细胞培养的系统

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Country Link
US (2) US20210123013A1 (https=)
EP (1) EP3797150A1 (https=)
JP (2) JP2021524253A (https=)
KR (2) KR20210011975A (https=)
CN (1) CN112424333A (https=)
AU (1) AU2019273006B2 (https=)
BR (1) BR112020023707A2 (https=)
CA (1) CA3101003A1 (https=)
CL (1) CL2020003003A1 (https=)
FR (1) FR3081168B1 (https=)
IL (2) IL325018A (https=)
MX (1) MX2020012412A (https=)
SG (1) SG11202011501XA (https=)
WO (1) WO2019224467A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114672455A (zh) * 2022-03-25 2022-06-28 中山大学 一种利用多能干细胞诱导骨髓基质细胞的方法
CN118308295A (zh) * 2024-06-06 2024-07-09 上海遇见味来生物技术有限公司 一种促进细胞生产胶原的添加剂、培养基及方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3122883A1 (fr) * 2021-05-11 2022-11-18 Treefrog Therapeutics Microcompartiments cellulaires comprenant des cellules dont l’intégrité génomique est maintenue après amplification et procédé de préparation
CN114214267B (zh) * 2021-12-06 2024-04-09 大连大学 一种类器官基质胶微球及其制备方法和应用
WO2023235884A1 (en) 2022-06-03 2023-12-07 Flagship Pioneering Innovations Vi, Llc Compositions and methods
FR3151044B1 (fr) * 2023-07-11 2026-04-24 Treefrog Therapeutics Transduction et/ou transfection dans un microcompartiment en trois dimensions
WO2025211257A1 (ja) * 2024-04-01 2025-10-09 慶應義塾 オルガノイド製造用培地及びその使用

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1635108A (zh) * 2004-11-25 2005-07-06 西安交通大学 一种旋转灌注式生物反应器系统
CN1898375A (zh) * 2003-10-21 2007-01-17 莱比锡大学 用于对具有三维形状、活性和机械抗力的细胞移植体进行培养和刺激的方法和生物反应器
CN101416059A (zh) * 2003-07-17 2009-04-22 格洛伯塞尔解决方案公司 自动化细胞培养系统及方法
CN102439135A (zh) * 2009-02-03 2012-05-02 荷兰皇家科学院 用于上皮干细胞和包含所述干细胞的类器官的培养基
CN103146572A (zh) * 2011-12-07 2013-06-12 清华大学 一种实现细胞集落均质性生长的装置和方法
CN103732733A (zh) * 2011-06-06 2014-04-16 里珍西斯私人有限公司 在中空纤维生物反应器中扩增干细胞
WO2016103002A1 (en) * 2014-12-22 2016-06-30 Ecole Polytechnique Federale De Lausanne (Epfl) Devices for high-throughput aggregation and manipulation of mammalian cells
CN107427537A (zh) * 2015-03-03 2017-12-01 哈佛学院院长及董事 产生功能性人体组织的方法
WO2018050862A1 (en) * 2016-09-19 2018-03-22 Ecole Polytechnique Fèdèrale De Lausanne Organoid arrays

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006136212A (ja) * 2004-11-10 2006-06-01 Olympus Corp 細胞培養用担体
US9109193B2 (en) * 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
JP2009247334A (ja) * 2008-04-11 2009-10-29 Toray Ind Inc 細胞培養担体
US9487756B2 (en) 2009-03-20 2016-11-08 Mesoblast, Inc. Production of reprogrammed pluripotent cells
RU2615179C2 (ru) * 2012-09-06 2017-04-04 Плуристем Лтд. Устройства и способы культивирования клеток
GB201706544D0 (en) * 2017-04-25 2017-06-07 Plasticell Ltd Method

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101416059A (zh) * 2003-07-17 2009-04-22 格洛伯塞尔解决方案公司 自动化细胞培养系统及方法
US20130189723A1 (en) * 2003-07-17 2013-07-25 Global Cell Solutions, Llc Automated cell culture system and process
CN1898375A (zh) * 2003-10-21 2007-01-17 莱比锡大学 用于对具有三维形状、活性和机械抗力的细胞移植体进行培养和刺激的方法和生物反应器
CN1635108A (zh) * 2004-11-25 2005-07-06 西安交通大学 一种旋转灌注式生物反应器系统
CN102439135A (zh) * 2009-02-03 2012-05-02 荷兰皇家科学院 用于上皮干细胞和包含所述干细胞的类器官的培养基
CN103732733A (zh) * 2011-06-06 2014-04-16 里珍西斯私人有限公司 在中空纤维生物反应器中扩增干细胞
CN103146572A (zh) * 2011-12-07 2013-06-12 清华大学 一种实现细胞集落均质性生长的装置和方法
WO2016103002A1 (en) * 2014-12-22 2016-06-30 Ecole Polytechnique Federale De Lausanne (Epfl) Devices for high-throughput aggregation and manipulation of mammalian cells
CN107427537A (zh) * 2015-03-03 2017-12-01 哈佛学院院长及董事 产生功能性人体组织的方法
WO2018050862A1 (en) * 2016-09-19 2018-03-22 Ecole Polytechnique Fèdèrale De Lausanne Organoid arrays

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
KEVIN ALESSANDRI等: "A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells(hNSC)", 《LAB ON A CHIP》 *
KEVIN ALESSANDRI等: "A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells(hNSC)", 《LAB ON A CHIP》, vol. 16, 21 March 2016 (2016-03-21), pages 1593, XP055393107, DOI: 10.1039/C6LC00133E *
MITSUE KOMATSU等: "Maturation of human iPS cell-derived dopamine neuron precursors in alginate-Ca(2+) hydrogel", 《BIOCHIM BIOPHYS ACTA》 *
MITSUE KOMATSU等: "Maturation of human iPS cell-derived dopamine neuron precursors in alginate-Ca(2+) hydrogel", 《BIOCHIM BIOPHYS ACTA》, vol. 1850, no. 9, 4 May 2015 (2015-05-04) *
TOMOAKI ASHIDA等: "Propagation of human iPS cells in alginate-based microcapsules prepared using reactions catalyzed by horseradish peroxidase and catalase", 《ARTIF CELLS NANOMED BIOTECHNOL》, vol. 44, no. 6, 6 July 2015 (2015-07-06) *
施新猷等主编: "《比较医学(上册)》", vol. 1, 陕西科学技术出版社, pages: 193 - 195 *
颜慧琼等: "海藻酸盐水凝胶作为组织工程支架材料改性与应用研究进展", 《化工新型材料》 *
颜慧琼等: "海藻酸盐水凝胶作为组织工程支架材料改性与应用研究进展", 《化工新型材料》, vol. 45, no. 3, 31 March 2017 (2017-03-31) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114672455A (zh) * 2022-03-25 2022-06-28 中山大学 一种利用多能干细胞诱导骨髓基质细胞的方法
CN118308295A (zh) * 2024-06-06 2024-07-09 上海遇见味来生物技术有限公司 一种促进细胞生产胶原的添加剂、培养基及方法

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Publication number Publication date
FR3081168A1 (fr) 2019-11-22
SG11202011501XA (en) 2020-12-30
US20250304909A1 (en) 2025-10-02
AU2019273006A1 (en) 2020-12-03
EP3797150A1 (fr) 2021-03-31
IL325018A (en) 2026-01-01
IL278775A (en) 2021-01-31
BR112020023707A2 (pt) 2021-02-09
FR3081168B1 (fr) 2022-03-11
AU2019273006B2 (en) 2024-11-28
JP2021524253A (ja) 2021-09-13
CA3101003A1 (fr) 2019-11-28
MX2020012412A (es) 2021-02-09
US20210123013A1 (en) 2021-04-29
JP2025128230A (ja) 2025-09-02
KR20250044479A (ko) 2025-03-31
IL278775B1 (en) 2026-01-01
KR20210011975A (ko) 2021-02-02
WO2019224467A1 (fr) 2019-11-28
CL2020003003A1 (es) 2021-04-16

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