JP2021524253A - バイオリアクターでの細胞培養システム - Google Patents

バイオリアクターでの細胞培養システム Download PDF

Info

Publication number
JP2021524253A
JP2021524253A JP2020565372A JP2020565372A JP2021524253A JP 2021524253 A JP2021524253 A JP 2021524253A JP 2020565372 A JP2020565372 A JP 2020565372A JP 2020565372 A JP2020565372 A JP 2020565372A JP 2021524253 A JP2021524253 A JP 2021524253A
Authority
JP
Japan
Prior art keywords
cells
microcompartment
cell
bioreactor
culture system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2020565372A
Other languages
English (en)
Japanese (ja)
Other versions
JP2021524253A5 (https=
JPWO2019224467A5 (https=
Inventor
マクシム・フェイユー
ケヴィン・アレッサンドリ
ピエール・ナソワ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut d'Optique Theorique et Appliquee
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut d'Optique Theorique et Appliquee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut d'Optique Theorique et Appliquee filed Critical Centre National de la Recherche Scientifique CNRS
Publication of JP2021524253A publication Critical patent/JP2021524253A/ja
Publication of JP2021524253A5 publication Critical patent/JP2021524253A5/ja
Publication of JPWO2019224467A5 publication Critical patent/JPWO2019224467A5/ja
Priority to JP2025092385A priority Critical patent/JP2025128230A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2020565372A 2018-05-21 2019-05-20 バイオリアクターでの細胞培養システム Pending JP2021524253A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025092385A JP2025128230A (ja) 2018-05-21 2025-06-03 バイオリアクターにおける細胞培養のためのシステム

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1854207A FR3081168B1 (fr) 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur
FR1854207 2018-05-21
PCT/FR2019/051144 WO2019224467A1 (fr) 2018-05-21 2019-05-20 Système de culture cellulaire en bioréacteur

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025092385A Division JP2025128230A (ja) 2018-05-21 2025-06-03 バイオリアクターにおける細胞培養のためのシステム

Publications (3)

Publication Number Publication Date
JP2021524253A true JP2021524253A (ja) 2021-09-13
JP2021524253A5 JP2021524253A5 (https=) 2022-05-27
JPWO2019224467A5 JPWO2019224467A5 (https=) 2022-05-27

Family

ID=64049308

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2020565372A Pending JP2021524253A (ja) 2018-05-21 2019-05-20 バイオリアクターでの細胞培養システム
JP2025092385A Pending JP2025128230A (ja) 2018-05-21 2025-06-03 バイオリアクターにおける細胞培養のためのシステム

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025092385A Pending JP2025128230A (ja) 2018-05-21 2025-06-03 バイオリアクターにおける細胞培養のためのシステム

Country Status (14)

Country Link
US (2) US20210123013A1 (https=)
EP (1) EP3797150A1 (https=)
JP (2) JP2021524253A (https=)
KR (2) KR20210011975A (https=)
CN (1) CN112424333A (https=)
AU (1) AU2019273006B2 (https=)
BR (1) BR112020023707A2 (https=)
CA (1) CA3101003A1 (https=)
CL (1) CL2020003003A1 (https=)
FR (1) FR3081168B1 (https=)
IL (2) IL325018A (https=)
MX (1) MX2020012412A (https=)
SG (1) SG11202011501XA (https=)
WO (1) WO2019224467A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025211257A1 (ja) * 2024-04-01 2025-10-09 慶應義塾 オルガノイド製造用培地及びその使用

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3122883A1 (fr) * 2021-05-11 2022-11-18 Treefrog Therapeutics Microcompartiments cellulaires comprenant des cellules dont l’intégrité génomique est maintenue après amplification et procédé de préparation
CN114214267B (zh) * 2021-12-06 2024-04-09 大连大学 一种类器官基质胶微球及其制备方法和应用
CN114672455B (zh) * 2022-03-25 2024-07-09 中山大学 一种利用多能干细胞诱导骨髓基质细胞的方法
US20250213470A1 (en) 2022-06-03 2025-07-03 Flagship Pioneering Innovations Vi, Llc Compositions and methods
FR3151044B1 (fr) * 2023-07-11 2026-04-24 Treefrog Therapeutics Transduction et/ou transfection dans un microcompartiment en trois dimensions
CN118308295B (zh) * 2024-06-06 2024-08-27 上海遇见味来生物技术有限公司 一种促进细胞生产胶原的添加剂、培养基及方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006136212A (ja) * 2004-11-10 2006-06-01 Olympus Corp 細胞培養用担体
JP2009247334A (ja) * 2008-04-11 2009-10-29 Toray Ind Inc 細胞培養担体
JP2015527075A (ja) * 2012-09-06 2015-09-17 プルリステム リミテッド 細胞の培養のためのデバイス及び方法
WO2016103002A1 (en) * 2014-12-22 2016-06-30 Ecole Polytechnique Federale De Lausanne (Epfl) Devices for high-throughput aggregation and manipulation of mammalian cells
WO2018050862A1 (en) * 2016-09-19 2018-03-22 Ecole Polytechnique Fèdèrale De Lausanne Organoid arrays

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004260106C1 (en) * 2003-07-17 2010-12-09 Global Cell Solutions, Llc. Automated cell culture system and process
DE10349484A1 (de) * 2003-10-21 2005-05-25 Universität Leipzig Verfahren und Bioreaktor zum Kultivieren und Stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen Zelltransplantaten
CN1288235C (zh) * 2004-11-25 2006-12-06 西安交通大学 一种旋转灌注式生物反应器系统
US9109193B2 (en) * 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
ES2579909T3 (es) * 2009-02-03 2016-08-17 Koninklijke Nederlandse Akademie Van Wetenschappen Medio de cultivo para células madre epiteliales y organoides que comprenden dichas células madre
CN102405280B (zh) 2009-03-20 2015-03-04 中胚层公司 重新设定的多能干细胞的制备
EP2718416B1 (en) * 2011-06-06 2019-11-13 Regenesys bvba Expansion of stem cells in hollow fiber bioreactors
CN103146572B (zh) * 2011-12-07 2016-01-06 清华大学 一种实现细胞集落均质性生长的装置和方法
JP6807853B2 (ja) * 2015-03-03 2021-01-06 プレジデント アンド フェローズ オブ ハーバード カレッジ 機能的ヒト組織の作製方法
GB201706544D0 (en) * 2017-04-25 2017-06-07 Plasticell Ltd Method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006136212A (ja) * 2004-11-10 2006-06-01 Olympus Corp 細胞培養用担体
JP2009247334A (ja) * 2008-04-11 2009-10-29 Toray Ind Inc 細胞培養担体
JP2015527075A (ja) * 2012-09-06 2015-09-17 プルリステム リミテッド 細胞の培養のためのデバイス及び方法
WO2016103002A1 (en) * 2014-12-22 2016-06-30 Ecole Polytechnique Federale De Lausanne (Epfl) Devices for high-throughput aggregation and manipulation of mammalian cells
WO2018050862A1 (en) * 2016-09-19 2018-03-22 Ecole Polytechnique Fèdèrale De Lausanne Organoid arrays

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAB ON A CHIP, vol. 16, JPN6024024317, 2016, pages 1593 - 1604, ISSN: 0005524921 *
STEM CELLS, vol. 22, JPN7024002645, 2004, pages 275 - 282, ISSN: 0005524922 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025211257A1 (ja) * 2024-04-01 2025-10-09 慶應義塾 オルガノイド製造用培地及びその使用

Also Published As

Publication number Publication date
EP3797150A1 (fr) 2021-03-31
WO2019224467A1 (fr) 2019-11-28
US20210123013A1 (en) 2021-04-29
IL278775B1 (en) 2026-01-01
IL325018A (en) 2026-01-01
AU2019273006A1 (en) 2020-12-03
FR3081168B1 (fr) 2022-03-11
JP2025128230A (ja) 2025-09-02
SG11202011501XA (en) 2020-12-30
KR20250044479A (ko) 2025-03-31
CL2020003003A1 (es) 2021-04-16
CN112424333A (zh) 2021-02-26
CA3101003A1 (fr) 2019-11-28
AU2019273006B2 (en) 2024-11-28
IL278775A (en) 2021-01-31
US20250304909A1 (en) 2025-10-02
BR112020023707A2 (pt) 2021-02-09
MX2020012412A (es) 2021-02-09
KR20210011975A (ko) 2021-02-02
FR3081168A1 (fr) 2019-11-22

Similar Documents

Publication Publication Date Title
JP2021524253A (ja) バイオリアクターでの細胞培養システム
US12146153B2 (en) Cellular microcompartment and preparation processes
JP2018534936A (ja) 大規模細胞製造システム
US20240043793A1 (en) Extracellular matrix substitute in a cellular microcompartment
Zhao et al. Application of stem cells in engineered vascular graft and vascularized organs
JP2025528743A (ja) 細胞微小区画における細胞外マトリックス代替物
Miranda et al. Human pluripotent stem cells: Applications and challenges for regenerative medicine and disease modeling
CN112852709B (zh) 小鼠肺类器官培养方法
JPWO2019224467A5 (https=)
JP2024521447A (ja) 複数の嚢胞を含む大型細胞微小区画
US8969079B2 (en) Method for inducing human blood-born hematospheres through aggregate culture and expanding blood adult stem cells and progenitor cells, and stem cell prepared by the same
CN117286106B (zh) 一种小鼠视网膜类器官的构建方法
WO2005121319A1 (en) Methods for production of mesodermal lineage cells
Borozenets et al. Features of spheroidogenesis of L929 cell line during prolonged three-dimensional cultivation
KR20250172953A (ko) 조성물
Willerth et al. Biomaterial Scaffolds for Human Embryonic Stem Cell Culture and Differentiation
RU2563347C2 (ru) Способ культивирования стволовых клеток, препятствующий их спонтанной дифференцировке (варианты)
Unsworth et al. Tissue assembly in microgravity
Polak et al. Cell expansion, cell encapsulation, 3D cultures
HK40015329A (en) Cellular microcompartment and preparation methods
NZ794660A (en) Cellular microcompartment and preparation methods
Akasha Entrapment of α-MHC [alpha-MHC] cardiomyocytes in CultiSpher-S microcarriers: preparation and characterization

Legal Events

Date Code Title Description
A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20220517

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220518

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220518

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230613

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20230906

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20231109

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20231208

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240206

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240430

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240625

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20240917

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241107

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20250212