FR3081168B1 - Systeme de culture cellulaire en bioreacteur - Google Patents

Systeme de culture cellulaire en bioreacteur Download PDF

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Publication number
FR3081168B1
FR3081168B1 FR1854207A FR1854207A FR3081168B1 FR 3081168 B1 FR3081168 B1 FR 3081168B1 FR 1854207 A FR1854207 A FR 1854207A FR 1854207 A FR1854207 A FR 1854207A FR 3081168 B1 FR3081168 B1 FR 3081168B1
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FR
France
Prior art keywords
cell culture
culture system
bioreactor cell
extracellular matrix
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1854207A
Other languages
English (en)
French (fr)
Other versions
FR3081168A1 (fr
Inventor
Maxime Feyeux
Kevin Alessandri
Pierre Nassoy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Bordeaux
Institut dOptique Graduate School
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Bordeaux
Institut dOptique Graduate School
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1854207A priority Critical patent/FR3081168B1/fr
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Bordeaux, Institut dOptique Graduate School filed Critical Centre National de la Recherche Scientifique CNRS
Priority to PCT/FR2019/051144 priority patent/WO2019224467A1/fr
Priority to CA3101003A priority patent/CA3101003A1/fr
Priority to AU2019273006A priority patent/AU2019273006B2/en
Priority to CN201980034566.7A priority patent/CN112424333A/zh
Priority to JP2020565372A priority patent/JP2021524253A/ja
Priority to KR1020207036522A priority patent/KR20210011975A/ko
Priority to US17/057,143 priority patent/US20210123013A1/en
Priority to MX2020012412A priority patent/MX2020012412A/es
Priority to BR112020023707-8A priority patent/BR112020023707A2/pt
Priority to EP19733852.8A priority patent/EP3797150A1/fr
Priority to KR1020257009299A priority patent/KR20250044479A/ko
Priority to IL325018A priority patent/IL325018A/en
Priority to SG11202011501XA priority patent/SG11202011501XA/en
Publication of FR3081168A1 publication Critical patent/FR3081168A1/fr
Priority to IL278775A priority patent/IL278775B1/en
Priority to CL2020003003A priority patent/CL2020003003A1/es
Application granted granted Critical
Publication of FR3081168B1 publication Critical patent/FR3081168B1/fr
Priority to JP2025092385A priority patent/JP2025128230A/ja
Priority to US19/233,809 priority patent/US20250304909A1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
FR1854207A 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur Active FR3081168B1 (fr)

Priority Applications (18)

Application Number Priority Date Filing Date Title
FR1854207A FR3081168B1 (fr) 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur
KR1020257009299A KR20250044479A (ko) 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템
AU2019273006A AU2019273006B2 (en) 2018-05-21 2019-05-20 System for cell culture in a bioreactor
CN201980034566.7A CN112424333A (zh) 2018-05-21 2019-05-20 用于在生物反应器中进行细胞培养的系统
JP2020565372A JP2021524253A (ja) 2018-05-21 2019-05-20 バイオリアクターでの細胞培養システム
KR1020207036522A KR20210011975A (ko) 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템
US17/057,143 US20210123013A1 (en) 2018-05-21 2019-05-20 System for cell culture in a bioreactor
MX2020012412A MX2020012412A (es) 2018-05-21 2019-05-20 Sistema de cultivo celular en biorreactor.
BR112020023707-8A BR112020023707A2 (pt) 2018-05-21 2019-05-20 sistema de cultura celular em biorreator
SG11202011501XA SG11202011501XA (en) 2018-05-21 2019-05-20 System for cell culture in a bioreactor
PCT/FR2019/051144 WO2019224467A1 (fr) 2018-05-21 2019-05-20 Système de culture cellulaire en bioréacteur
CA3101003A CA3101003A1 (fr) 2018-05-21 2019-05-20 Systeme de culture cellulaire en bioreacteur
EP19733852.8A EP3797150A1 (fr) 2018-05-21 2019-05-20 Système de culture cellulaire en bioréacteur
IL325018A IL325018A (en) 2018-05-21 2019-05-20 System for cell culture in a bioreactor
IL278775A IL278775B1 (en) 2018-05-21 2020-11-17 System for cell culture in a bioreactor
CL2020003003A CL2020003003A1 (es) 2018-05-21 2020-11-18 Sistema de cultivo celular en biorreactor
JP2025092385A JP2025128230A (ja) 2018-05-21 2025-06-03 バイオリアクターにおける細胞培養のためのシステム
US19/233,809 US20250304909A1 (en) 2018-05-21 2025-06-10 System for cell culture in a bioreactor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1854207 2018-05-21
FR1854207A FR3081168B1 (fr) 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur

Publications (2)

Publication Number Publication Date
FR3081168A1 FR3081168A1 (fr) 2019-11-22
FR3081168B1 true FR3081168B1 (fr) 2022-03-11

Family

ID=64049308

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1854207A Active FR3081168B1 (fr) 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur

Country Status (14)

Country Link
US (2) US20210123013A1 (https=)
EP (1) EP3797150A1 (https=)
JP (2) JP2021524253A (https=)
KR (2) KR20210011975A (https=)
CN (1) CN112424333A (https=)
AU (1) AU2019273006B2 (https=)
BR (1) BR112020023707A2 (https=)
CA (1) CA3101003A1 (https=)
CL (1) CL2020003003A1 (https=)
FR (1) FR3081168B1 (https=)
IL (2) IL325018A (https=)
MX (1) MX2020012412A (https=)
SG (1) SG11202011501XA (https=)
WO (1) WO2019224467A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3122883A1 (fr) * 2021-05-11 2022-11-18 Treefrog Therapeutics Microcompartiments cellulaires comprenant des cellules dont l’intégrité génomique est maintenue après amplification et procédé de préparation
CN114214267B (zh) * 2021-12-06 2024-04-09 大连大学 一种类器官基质胶微球及其制备方法和应用
CN114672455B (zh) * 2022-03-25 2024-07-09 中山大学 一种利用多能干细胞诱导骨髓基质细胞的方法
WO2023235884A1 (en) 2022-06-03 2023-12-07 Flagship Pioneering Innovations Vi, Llc Compositions and methods
FR3151044B1 (fr) * 2023-07-11 2026-04-24 Treefrog Therapeutics Transduction et/ou transfection dans un microcompartiment en trois dimensions
WO2025211257A1 (ja) * 2024-04-01 2025-10-09 慶應義塾 オルガノイド製造用培地及びその使用
CN118308295B (zh) * 2024-06-06 2024-08-27 上海遇见味来生物技术有限公司 一种促进细胞生产胶原的添加剂、培养基及方法

Family Cites Families (15)

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CA2532754A1 (en) * 2003-07-17 2005-02-03 Global Cell Solutions, Llc Automated cell culture system and process
DE10349484A1 (de) * 2003-10-21 2005-05-25 Universität Leipzig Verfahren und Bioreaktor zum Kultivieren und Stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen Zelltransplantaten
JP2006136212A (ja) * 2004-11-10 2006-06-01 Olympus Corp 細胞培養用担体
CN1288235C (zh) * 2004-11-25 2006-12-06 西安交通大学 一种旋转灌注式生物反应器系统
US9109193B2 (en) * 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
JP2009247334A (ja) * 2008-04-11 2009-10-29 Toray Ind Inc 細胞培養担体
EP2393917B1 (en) * 2009-02-03 2016-04-06 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells.
US9487756B2 (en) 2009-03-20 2016-11-08 Mesoblast, Inc. Production of reprogrammed pluripotent cells
PL2718416T3 (pl) * 2011-06-06 2020-05-18 ReGenesys BVBA Namnażanie komórek macierzystych w bioreaktorach włóknisto-kapilarnych
CN103146572B (zh) * 2011-12-07 2016-01-06 清华大学 一种实现细胞集落均质性生长的装置和方法
RU2615179C2 (ru) * 2012-09-06 2017-04-04 Плуристем Лтд. Устройства и способы культивирования клеток
JP7089240B2 (ja) * 2014-12-22 2022-06-22 エコール・ポリテクニーク・フェデラル・ドゥ・ローザンヌ （ウ・ペ・エフ・エル） 高い収集能力で哺乳動物細胞を操作（マニピュレーション）する装置
CN107427537A (zh) * 2015-03-03 2017-12-01 哈佛学院院长及董事 产生功能性人体组织的方法
EP3296018A1 (en) * 2016-09-19 2018-03-21 Ecole Polytechnique Fédérale de Lausanne (EPFL) Organoid arrays
GB201706544D0 (en) * 2017-04-25 2017-06-07 Plasticell Ltd Method

Also Published As

Publication number Publication date
FR3081168A1 (fr) 2019-11-22
SG11202011501XA (en) 2020-12-30
US20250304909A1 (en) 2025-10-02
AU2019273006A1 (en) 2020-12-03
EP3797150A1 (fr) 2021-03-31
IL325018A (en) 2026-01-01
IL278775A (en) 2021-01-31
BR112020023707A2 (pt) 2021-02-09
AU2019273006B2 (en) 2024-11-28
JP2021524253A (ja) 2021-09-13
CA3101003A1 (fr) 2019-11-28
MX2020012412A (es) 2021-02-09
US20210123013A1 (en) 2021-04-29
JP2025128230A (ja) 2025-09-02
CN112424333A (zh) 2021-02-26
KR20250044479A (ko) 2025-03-31
IL278775B1 (en) 2026-01-01
KR20210011975A (ko) 2021-02-02
WO2019224467A1 (fr) 2019-11-28
CL2020003003A1 (es) 2021-04-16

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