KR20210011975A - 생물반응기에서 세포 배양을 위한 시스템 - Google Patents

생물반응기에서 세포 배양을 위한 시스템 Download PDF

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Publication number
KR20210011975A
KR20210011975A KR1020207036522A KR20207036522A KR20210011975A KR 20210011975 A KR20210011975 A KR 20210011975A KR 1020207036522 A KR1020207036522 A KR 1020207036522A KR 20207036522 A KR20207036522 A KR 20207036522A KR 20210011975 A KR20210011975 A KR 20210011975A
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KR
South Korea
Prior art keywords
cells
bioreactor
microcompartments
cell
microcompartment
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Ceased
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KR1020207036522A
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English (en)
Korean (ko)
Inventor
막심 페이유
케뱅 알렉상드리
피에르 나수아
Original Assignee
유니베르시떼 드 보르도
앵스띠뛰 도프띠끄 테오리끄 에 아플리끄
상뜨르 나쇼날 드 라 러쉐르쉬 샹띠피끄
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Application filed by 유니베르시떼 드 보르도, 앵스띠뛰 도프띠끄 테오리끄 에 아플리끄, 상뜨르 나쇼날 드 라 러쉐르쉬 샹띠피끄 filed Critical 유니베르시떼 드 보르도
Priority to KR1020257009299A priority Critical patent/KR20250044479A/ko
Publication of KR20210011975A publication Critical patent/KR20210011975A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/10—Bioreactors or fermenters specially adapted for specific uses adapted for the cultivation of avian eggs or in avian eggs, e.g. for vaccine production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020207036522A 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템 Ceased KR20210011975A (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020257009299A KR20250044479A (ko) 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1854207A FR3081168B1 (fr) 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur
FR1854207 2018-05-21
PCT/FR2019/051144 WO2019224467A1 (fr) 2018-05-21 2019-05-20 Système de culture cellulaire en bioréacteur

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020257009299A Division KR20250044479A (ko) 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템

Publications (1)

Publication Number Publication Date
KR20210011975A true KR20210011975A (ko) 2021-02-02

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ID=64049308

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020207036522A Ceased KR20210011975A (ko) 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템
KR1020257009299A Ceased KR20250044479A (ko) 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020257009299A Ceased KR20250044479A (ko) 2018-05-21 2019-05-20 생물반응기에서 세포 배양을 위한 시스템

Country Status (14)

Country Link
US (2) US20210123013A1 (https=)
EP (1) EP3797150A1 (https=)
JP (2) JP2021524253A (https=)
KR (2) KR20210011975A (https=)
CN (1) CN112424333A (https=)
AU (1) AU2019273006B2 (https=)
BR (1) BR112020023707A2 (https=)
CA (1) CA3101003A1 (https=)
CL (1) CL2020003003A1 (https=)
FR (1) FR3081168B1 (https=)
IL (2) IL325018A (https=)
MX (1) MX2020012412A (https=)
SG (1) SG11202011501XA (https=)
WO (1) WO2019224467A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3122883A1 (fr) * 2021-05-11 2022-11-18 Treefrog Therapeutics Microcompartiments cellulaires comprenant des cellules dont l’intégrité génomique est maintenue après amplification et procédé de préparation
CN114214267B (zh) * 2021-12-06 2024-04-09 大连大学 一种类器官基质胶微球及其制备方法和应用
CN114672455B (zh) * 2022-03-25 2024-07-09 中山大学 一种利用多能干细胞诱导骨髓基质细胞的方法
US20250213470A1 (en) 2022-06-03 2025-07-03 Flagship Pioneering Innovations Vi, Llc Compositions and methods
FR3151044B1 (fr) * 2023-07-11 2026-04-24 Treefrog Therapeutics Transduction et/ou transfection dans un microcompartiment en trois dimensions
WO2025211257A1 (ja) * 2024-04-01 2025-10-09 慶應義塾 オルガノイド製造用培地及びその使用
CN118308295B (zh) * 2024-06-06 2024-08-27 上海遇见味来生物技术有限公司 一种促进细胞生产胶原的添加剂、培养基及方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004260106C1 (en) * 2003-07-17 2010-12-09 Global Cell Solutions, Llc. Automated cell culture system and process
DE10349484A1 (de) * 2003-10-21 2005-05-25 Universität Leipzig Verfahren und Bioreaktor zum Kultivieren und Stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen Zelltransplantaten
JP2006136212A (ja) * 2004-11-10 2006-06-01 Olympus Corp 細胞培養用担体
CN1288235C (zh) * 2004-11-25 2006-12-06 西安交通大学 一种旋转灌注式生物反应器系统
US9109193B2 (en) * 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
JP2009247334A (ja) * 2008-04-11 2009-10-29 Toray Ind Inc 細胞培養担体
ES2579909T3 (es) * 2009-02-03 2016-08-17 Koninklijke Nederlandse Akademie Van Wetenschappen Medio de cultivo para células madre epiteliales y organoides que comprenden dichas células madre
CN102405280B (zh) 2009-03-20 2015-03-04 中胚层公司 重新设定的多能干细胞的制备
EP2718416B1 (en) * 2011-06-06 2019-11-13 Regenesys bvba Expansion of stem cells in hollow fiber bioreactors
CN103146572B (zh) * 2011-12-07 2016-01-06 清华大学 一种实现细胞集落均质性生长的装置和方法
EP2902478A1 (en) * 2012-09-06 2015-08-05 Pluristem Ltd. Devices and methods for culture of cells
US11583860B2 (en) * 2014-12-22 2023-02-21 Ecole Polytechnique Federale De Lausanne (Epfl) Microstructured thin hydrogel films
JP6807853B2 (ja) * 2015-03-03 2021-01-06 プレジデント アンド フェローズ オブ ハーバード カレッジ 機能的ヒト組織の作製方法
EP3296018A1 (en) * 2016-09-19 2018-03-21 Ecole Polytechnique Fédérale de Lausanne (EPFL) Organoid arrays
GB201706544D0 (en) * 2017-04-25 2017-06-07 Plasticell Ltd Method

Also Published As

Publication number Publication date
EP3797150A1 (fr) 2021-03-31
WO2019224467A1 (fr) 2019-11-28
US20210123013A1 (en) 2021-04-29
IL278775B1 (en) 2026-01-01
IL325018A (en) 2026-01-01
AU2019273006A1 (en) 2020-12-03
FR3081168B1 (fr) 2022-03-11
JP2025128230A (ja) 2025-09-02
SG11202011501XA (en) 2020-12-30
KR20250044479A (ko) 2025-03-31
JP2021524253A (ja) 2021-09-13
CL2020003003A1 (es) 2021-04-16
CN112424333A (zh) 2021-02-26
CA3101003A1 (fr) 2019-11-28
AU2019273006B2 (en) 2024-11-28
IL278775A (en) 2021-01-31
US20250304909A1 (en) 2025-10-02
BR112020023707A2 (pt) 2021-02-09
MX2020012412A (es) 2021-02-09
FR3081168A1 (fr) 2019-11-22

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