CA3101003A1 - Systeme de culture cellulaire en bioreacteur - Google Patents

Systeme de culture cellulaire en bioreacteur Download PDF

Info

Publication number
CA3101003A1
CA3101003A1 CA3101003A CA3101003A CA3101003A1 CA 3101003 A1 CA3101003 A1 CA 3101003A1 CA 3101003 A CA3101003 A CA 3101003A CA 3101003 A CA3101003 A CA 3101003A CA 3101003 A1 CA3101003 A1 CA 3101003A1
Authority
CA
Canada
Prior art keywords
cells
microcompartments
bioreactor
cell
cellular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3101003A
Other languages
English (en)
French (fr)
Inventor
Maxime FEYEUX
Kevin ALESSANDRI
Pierre Nassoy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut d'Optique Theorique et Appliquee
Universite de Bordeaux
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut d'Optique Theorique et Appliquee
Universite de Bordeaux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut d'Optique Theorique et Appliquee, Universite de Bordeaux filed Critical Centre National de la Recherche Scientifique CNRS
Publication of CA3101003A1 publication Critical patent/CA3101003A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3101003A 2018-05-21 2019-05-20 Systeme de culture cellulaire en bioreacteur Pending CA3101003A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1854207A FR3081168B1 (fr) 2018-05-21 2018-05-21 Systeme de culture cellulaire en bioreacteur
FR1854207 2018-05-21
PCT/FR2019/051144 WO2019224467A1 (fr) 2018-05-21 2019-05-20 Système de culture cellulaire en bioréacteur

Publications (1)

Publication Number Publication Date
CA3101003A1 true CA3101003A1 (fr) 2019-11-28

Family

ID=64049308

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3101003A Pending CA3101003A1 (fr) 2018-05-21 2019-05-20 Systeme de culture cellulaire en bioreacteur

Country Status (14)

Country Link
US (2) US20210123013A1 (https=)
EP (1) EP3797150A1 (https=)
JP (2) JP2021524253A (https=)
KR (2) KR20210011975A (https=)
CN (1) CN112424333A (https=)
AU (1) AU2019273006B2 (https=)
BR (1) BR112020023707A2 (https=)
CA (1) CA3101003A1 (https=)
CL (1) CL2020003003A1 (https=)
FR (1) FR3081168B1 (https=)
IL (2) IL325018A (https=)
MX (1) MX2020012412A (https=)
SG (1) SG11202011501XA (https=)
WO (1) WO2019224467A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3122883A1 (fr) * 2021-05-11 2022-11-18 Treefrog Therapeutics Microcompartiments cellulaires comprenant des cellules dont l’intégrité génomique est maintenue après amplification et procédé de préparation
CN114214267B (zh) * 2021-12-06 2024-04-09 大连大学 一种类器官基质胶微球及其制备方法和应用
CN114672455B (zh) * 2022-03-25 2024-07-09 中山大学 一种利用多能干细胞诱导骨髓基质细胞的方法
US20250213470A1 (en) 2022-06-03 2025-07-03 Flagship Pioneering Innovations Vi, Llc Compositions and methods
FR3151044B1 (fr) * 2023-07-11 2026-04-24 Treefrog Therapeutics Transduction et/ou transfection dans un microcompartiment en trois dimensions
WO2025211257A1 (ja) * 2024-04-01 2025-10-09 慶應義塾 オルガノイド製造用培地及びその使用
CN118308295B (zh) * 2024-06-06 2024-08-27 上海遇见味来生物技术有限公司 一种促进细胞生产胶原的添加剂、培养基及方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004260106C1 (en) * 2003-07-17 2010-12-09 Global Cell Solutions, Llc. Automated cell culture system and process
DE10349484A1 (de) * 2003-10-21 2005-05-25 Universität Leipzig Verfahren und Bioreaktor zum Kultivieren und Stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen Zelltransplantaten
JP2006136212A (ja) * 2004-11-10 2006-06-01 Olympus Corp 細胞培養用担体
CN1288235C (zh) * 2004-11-25 2006-12-06 西安交通大学 一种旋转灌注式生物反应器系统
US9109193B2 (en) * 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
JP2009247334A (ja) * 2008-04-11 2009-10-29 Toray Ind Inc 細胞培養担体
ES2579909T3 (es) * 2009-02-03 2016-08-17 Koninklijke Nederlandse Akademie Van Wetenschappen Medio de cultivo para células madre epiteliales y organoides que comprenden dichas células madre
CN102405280B (zh) 2009-03-20 2015-03-04 中胚层公司 重新设定的多能干细胞的制备
EP2718416B1 (en) * 2011-06-06 2019-11-13 Regenesys bvba Expansion of stem cells in hollow fiber bioreactors
CN103146572B (zh) * 2011-12-07 2016-01-06 清华大学 一种实现细胞集落均质性生长的装置和方法
EP2902478A1 (en) * 2012-09-06 2015-08-05 Pluristem Ltd. Devices and methods for culture of cells
US11583860B2 (en) * 2014-12-22 2023-02-21 Ecole Polytechnique Federale De Lausanne (Epfl) Microstructured thin hydrogel films
JP6807853B2 (ja) * 2015-03-03 2021-01-06 プレジデント アンド フェローズ オブ ハーバード カレッジ 機能的ヒト組織の作製方法
EP3296018A1 (en) * 2016-09-19 2018-03-21 Ecole Polytechnique Fédérale de Lausanne (EPFL) Organoid arrays
GB201706544D0 (en) * 2017-04-25 2017-06-07 Plasticell Ltd Method

Also Published As

Publication number Publication date
EP3797150A1 (fr) 2021-03-31
WO2019224467A1 (fr) 2019-11-28
US20210123013A1 (en) 2021-04-29
IL278775B1 (en) 2026-01-01
IL325018A (en) 2026-01-01
AU2019273006A1 (en) 2020-12-03
FR3081168B1 (fr) 2022-03-11
JP2025128230A (ja) 2025-09-02
SG11202011501XA (en) 2020-12-30
KR20250044479A (ko) 2025-03-31
JP2021524253A (ja) 2021-09-13
CL2020003003A1 (es) 2021-04-16
CN112424333A (zh) 2021-02-26
AU2019273006B2 (en) 2024-11-28
IL278775A (en) 2021-01-31
US20250304909A1 (en) 2025-10-02
BR112020023707A2 (pt) 2021-02-09
MX2020012412A (es) 2021-02-09
KR20210011975A (ko) 2021-02-02
FR3081168A1 (fr) 2019-11-22

Similar Documents

Publication Publication Date Title
CA3101003A1 (fr) Systeme de culture cellulaire en bioreacteur
EP3545080B1 (fr) Microcompartiment cellulaire et procédés de préparation
EP4337761A1 (fr) Microcompartiments cellulaires comprenant des cellules dont l'intégrité génomique est maintenue apres amplification et procede de preparation
EP4619505A1 (fr) Substitut de matrice extracellulaire dans un microcompartiment cellulaire
WO2024033284A1 (fr) Substitut de matrice extracellulaire dans un microcompartiment cellulaire
CA3222350A1 (fr) Microcompartiments cellulaires de grande taille comprenant plusieurs cystes
FR3138661A1 (fr) Substitut de matrice extracellulaire dans un microcompartiment cellulaire
WO2021004974A1 (fr) Procede de production in vitro de neurones de mammiferes
JPWO2019224467A5 (https=)
FR3134117A1 (fr) Microcompartiments cellulaires comprenant des lymphocytes adaptés pour la culture à grande échelle
CA3192855A1 (fr) Tissu cardiaque particulier et utilisation dans le traitement de pathologies cardiaque
EP4214305A1 (fr) Microcompartiments cellulaires comprenant des cellules humaines en cours de differenciation cardiaque, tissus obtenus a partir de ces microcompartiments et utilisations
WO2025012400A1 (fr) Transduction et/ou transfection dans un microcompartiment en trois dimensions
FR3124193A3 (fr) Microcompartiments cellulaires de grande taille comprenant plusieurs cystes
FR3134114A1 (fr) Microcompartiments cellulaires comprenant des lymphocytes adaptés pour la culture à grande échelle
EP4504912A1 (fr) Microcompartiments cellulaires comprenant des lymphocytes formant une culture groupee en 3d et ayant une faible teneur en granzyme b
FR3134115A1 (fr) Microcompartiments cellulaires comprenant des lymphocytes formant une culture groupée en 3D et ayant une faible teneur en granzyme B

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220929

B12 Application deemed to be withdrawn, abandoned or lapsed

Free format text: ST27 STATUS EVENT CODE: N-2-6-B10-B12-B303 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO AN EXAMINER'S REQUISITION

Effective date: 20240829

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD

Year of fee payment: 6

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: N-6-6-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250513

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: N-6-6-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250513