CN112204136A - 植酸酶突变体 - Google Patents
植酸酶突变体 Download PDFInfo
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- CN112204136A CN112204136A CN201980036097.2A CN201980036097A CN112204136A CN 112204136 A CN112204136 A CN 112204136A CN 201980036097 A CN201980036097 A CN 201980036097A CN 112204136 A CN112204136 A CN 112204136A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
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Abstract
本发明涉及热稳定性植酸酶，在野生型大肠杆菌植酸酶或突变型大肠杆菌植酸酶的氨基酸序列中包含至少一对引入的二硫键，引入后可以提升植酸酶的性质，特别是在于耐热稳定性，耐蒸汽稳定性，制粒稳定性方面，显著优于现有的野生型或者突变型植酸酶；相比于现有的引入二硫键的工程改造植酸酶，其耐热稳定性也有显著提高。
Description
PCT国内申请，说明书已公开。
Claims (48)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810540167 | 2018-05-30 | ||
| CN2018105401675 | 2018-05-30 | ||
| PCT/CN2019/089212 WO2019228441A1 (zh) | 2018-05-30 | 2019-05-30 | 植酸酶突变体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112204136A true CN112204136A (zh) | 2021-01-08 |
| CN112204136B CN112204136B (zh) | 2024-05-14 |
Family
ID=68698518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980036097.2A Active CN112204136B (zh) | 2018-05-30 | 2019-05-30 | 植酸酶突变体 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20210207112A1 (zh) |
| EP (1) | EP3805381A4 (zh) |
| CN (1) | CN112204136B (zh) |
| EA (1) | EA202092569A1 (zh) |
| TW (1) | TW202012621A (zh) |
| WO (1) | WO2019228441A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108603181A (zh) * | 2016-06-30 | 2018-09-28 | 福尼亚生物处理股份有限公司 | 新植酸酶及其用途 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117535266A (zh) * | 2020-04-29 | 2024-02-09 | 南京百斯杰生物工程有限公司 | 一种亲本植酸酶变体 |
| US20220049230A1 (en) * | 2020-08-13 | 2022-02-17 | Novozymes A/S | Phytase variants and polynucleotides encoding same |
| EP4370670A1 (en) * | 2021-07-16 | 2024-05-22 | AB Enzymes Oy | Phytase variants with improved stability and ip4 activity |
| EP4119661A1 (en) * | 2021-07-16 | 2023-01-18 | AB Enzymes Oy | Phytase variants with improved stability and ip4 activity |
| EP4119658A1 (en) * | 2021-07-16 | 2023-01-18 | AB Enzymes Oy | Phytase variants |
| KR20230048891A (ko) * | 2021-10-05 | 2023-04-12 | 씨제이제일제당 (주) | 피타아제 변이체 |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102002487A (zh) * | 2010-11-03 | 2011-04-06 | 广东溢多利生物科技股份有限公司 | 一种优化改良的耐高温植酸酶phyth及其基因和应用 |
| CN102573514A (zh) * | 2007-12-03 | 2012-07-11 | 先正达参股股份有限公司 | 工程化改造酶易感性肌醇六磷酸酶 |
| CN102906254A (zh) * | 2010-03-26 | 2013-01-30 | 诺维信公司 | 热稳定性肌醇六磷酸酶变体 |
| CN102906255A (zh) * | 2010-03-26 | 2013-01-30 | 诺维信公司 | 热稳定性肌醇六磷酸酶变体 |
| CN102943083A (zh) * | 2012-11-27 | 2013-02-27 | 青岛根源生物技术集团有限公司 | 一种定点突变的耐高温植酸酶基因tp及其表达载体和应用 |
| CN105219749A (zh) * | 2015-11-04 | 2016-01-06 | 广东溢多利生物科技股份有限公司 | 优化改良的植酸酶突变体及其编码基因和应用 |
| CN105624131A (zh) * | 2014-11-21 | 2016-06-01 | 青岛蔚蓝生物集团有限公司 | 植酸酶突变体 |
| CN105969750A (zh) * | 2016-06-24 | 2016-09-28 | 北京昕大洋科技发展有限公司 | 一种植酸酶突变体及其应用 |
| US20170240872A1 (en) * | 2016-02-18 | 2017-08-24 | Dongguan APAC Biotechnology Co., Ltd. | Phytase having improved thermostability |
| CN107236717A (zh) * | 2016-03-28 | 2017-10-10 | 青岛蔚蓝生物集团有限公司 | 植酸酶突变体 |
| CN107353327A (zh) * | 2017-03-30 | 2017-11-17 | 南京百斯杰生物工程有限公司 | 植酸酶在黑曲霉中表达 |
| CN107809912A (zh) * | 2015-07-02 | 2018-03-16 | 诺维信公司 | 动物饲料组合物及其用途 |
Family Cites Families (8)
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Also Published As
| Publication number | Publication date |
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| TW202012621A (zh) | 2020-04-01 |
| WO2019228441A1 (zh) | 2019-12-05 |
| EP3805381A1 (en) | 2021-04-14 |
| CN112204136B (zh) | 2024-05-14 |
| EA202092569A1 (ru) | 2021-07-08 |
| EP3805381A4 (en) | 2022-04-20 |
| US20210207112A1 (en) | 2021-07-08 |
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