CN112175844A - Preparation method of microbial deodorant - Google Patents
Preparation method of microbial deodorant Download PDFInfo
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- CN112175844A CN112175844A CN202011090788.1A CN202011090788A CN112175844A CN 112175844 A CN112175844 A CN 112175844A CN 202011090788 A CN202011090788 A CN 202011090788A CN 112175844 A CN112175844 A CN 112175844A
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- 239000002781 deodorant agent Substances 0.000 title claims abstract description 43
- 230000000813 microbial effect Effects 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims abstract description 84
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 48
- 238000000855 fermentation Methods 0.000 claims abstract description 46
- 230000004151 fermentation Effects 0.000 claims abstract description 46
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 40
- 239000001963 growth medium Substances 0.000 claims abstract description 35
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 27
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 27
- 239000000725 suspension Substances 0.000 claims abstract description 26
- 238000002156 mixing Methods 0.000 claims abstract description 22
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 21
- 239000004310 lactic acid Substances 0.000 claims abstract description 21
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 20
- 235000015193 tomato juice Nutrition 0.000 claims abstract description 18
- 244000068988 Glycine max Species 0.000 claims abstract description 17
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 17
- 239000011780 sodium chloride Substances 0.000 claims abstract description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 14
- 239000002609 medium Substances 0.000 claims abstract description 14
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims abstract description 11
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims abstract description 11
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 11
- 229910052938 sodium sulfate Inorganic materials 0.000 claims abstract description 11
- 235000011152 sodium sulphate Nutrition 0.000 claims abstract description 11
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 8
- 238000007865 diluting Methods 0.000 claims abstract description 8
- 230000001502 supplementing effect Effects 0.000 claims abstract description 8
- 241000186660 Lactobacillus Species 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 7
- 150000001875 compounds Chemical class 0.000 claims abstract description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 3
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 30
- 239000008103 glucose Substances 0.000 claims description 30
- 239000002994 raw material Substances 0.000 claims description 20
- 229940041514 candida albicans extract Drugs 0.000 claims description 15
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 15
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 15
- 239000012138 yeast extract Substances 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 235000013312 flour Nutrition 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- 235000019764 Soybean Meal Nutrition 0.000 claims description 9
- 239000004455 soybean meal Substances 0.000 claims description 9
- 241000179039 Paenibacillus Species 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 239000003085 diluting agent Substances 0.000 claims description 5
- 235000019262 disodium citrate Nutrition 0.000 claims description 5
- 239000002526 disodium citrate Substances 0.000 claims description 5
- 229940079896 disodium hydrogen citrate Drugs 0.000 claims description 5
- CEYULKASIQJZGP-UHFFFAOYSA-L disodium;2-(carboxymethyl)-2-hydroxybutanedioate Chemical compound [Na+].[Na+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O CEYULKASIQJZGP-UHFFFAOYSA-L 0.000 claims description 5
- 210000002969 egg yolk Anatomy 0.000 claims description 5
- 244000144972 livestock Species 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 210000002700 urine Anatomy 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 3
- 230000001877 deodorizing effect Effects 0.000 claims description 3
- 239000010791 domestic waste Substances 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 8
- 238000004332 deodorization Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 7
- 239000002207 metabolite Substances 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 21
- 239000002131 composite material Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 235000013345 egg yolk Nutrition 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses a preparation method of a microbial deodorant, which comprises the following steps: (1) preparing a fermentation medium: decocting soybean sprout in water to obtain base solution; diluting the base solution, and mixing with tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate to obtain a fermentation medium; (2) photosynthetic bacteria fermentation: inoculating photosynthetic bacteria into a fermentation culture medium, and culturing to obtain photosynthetic bacteria suspension; (3) fermenting by using compound bacteria: respectively carrying out propagation on nitrogen-fixing bacillus, bacillus subtilis, saccharomycetes and lactic acid bacteria; inoculating the bacillus azotobacteria seed liquid, the bacillus subtilis seed liquid, the yeast seed liquid and the lactobacillus seed liquid which are obtained by propagation into the photosynthetic bacteria suspension, supplementing a carbon source, and fermenting to obtain the microbial deodorant. The invention ferments a plurality of microorganisms in the same system, has convenient operation, produces the metabolite and the flora to play a synergistic effect, and has good deodorization effect.
Description
Technical Field
The invention relates to the technical field of biological deodorization, in particular to a preparation method of a microbial deodorant.
Background
The deodorant has the functions of deodorizing, beautifying the environment and the like, and mainly comprises a physical deodorant, a chemical deodorant, a microbial deodorant, a plant deodorant and a compound deodorant. The microbial deodorant is characterized in that the microbial deodorant is prepared by using the physiological metabolic activity of microbes, so that substances with foul odor are converted into odorless substances, and the microbial deodorant is safe, efficient, environment-friendly and low in price.
Because the components of the domestic waste liquid or the livestock excrement and urine are relatively complex, the deodorization effect of a single strain is not obvious under general conditions; although the composite microbial deodorization effect is better than that of a single bacterial strain, because the fermentation conditions of all the bacteria are different and antagonism may exist, the bacteria need to be fermented independently and then compounded, the process is complex and the cost is higher.
Therefore, how to provide a composite microbial deodorant which is simple to prepare and has remarkable deodorization effect is a problem to be solved by the technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a preparation method of a microbial deodorant, which is convenient to operate because a plurality of microbes are fermented in the same system, and the produced metabolites and flora play a synergistic effect, so that the deodorant effect is better.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a microbial deodorant comprises the following steps:
(1) preparing a fermentation medium:
decocting soybean sprout in water to obtain base solution;
diluting the base solution, and mixing with tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate to obtain a fermentation medium;
(2) photosynthetic bacteria fermentation:
inoculating photosynthetic bacteria into a fermentation culture medium, and culturing to obtain photosynthetic bacteria suspension;
(3) fermenting by using compound bacteria:
respectively carrying out propagation on nitrogen-fixing bacillus, bacillus subtilis, saccharomycetes and lactic acid bacteria;
inoculating the bacillus azotobacteria seed liquid, the bacillus subtilis seed liquid, the yeast seed liquid and the lactobacillus seed liquid which are obtained by propagation into the photosynthetic bacteria suspension, supplementing a carbon source, and fermenting to obtain the microbial deodorant.
Preferably, in the step (1),
the mass ratio of the soybean sprouts to water used in the soybean sprout decoction is 1: (1.1-1.4);
decocting and boiling for more than 30 min.
Preferably, in the step (1),
the base liquid is prepared according to the following steps of 1: (10-15) diluting in a mass ratio, wherein the mass ratio of the obtained diluent to other components in the fermentation medium is 1: (1.2-1.5);
the mass ratio of tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate is (15-20): (9-12): (0.5-0.8): (0.04-0.07).
The soybean sprouts and the tomato juice used in the invention are natural, safe and nutritional; the soybean sprouts contain rich protein and trace elements, and the boiled water obtained by boiling the soybean sprouts is used as a base solution to prepare a fermentation medium, so that the growth requirements of various bacteria can be met; the tomato juice contains abundant vitamins and trace elements, and can promote the growth and metabolism of various bacteria in the same fermentation system, so that the fermentation efficiency and the application effect of the deodorant are ensured. The fermentation of photosynthetic bacteria is firstly carried out, and then the fermentation of nitrogen-fixing bacillus, bacillus subtilis, saccharomycetes and lactic acid bacteria is carried out, so that the high-efficiency propagation of each bacterium is ensured, metabolites such as acid, ketone and the like are produced, a material basis is further provided for the conversion of organic matters during deodorization, and the synergistic effect of each flora is promoted.
The nitrogen-fixing Paenibacillus can fully release nitrogen and phosphorus in the water body, and the combined action of the microzyme, the lactic acid bacteria and the photosynthetic bacteria is favorable for absorbing and decomposing SO2、H2S and other harmful gases with foul smell, thereby achieving the effect of deodorization. The secondary metabolite of Bacillus subtilis can provide nutrition for yeast, lactobacillus, and photosynthetic bacteria.
Preferably, in the step (2),
inoculating photosynthetic bacteria in fermentation culture medium at 15-20 wt%, and culturing to obtain photosynthetic bacteria suspension with thallus concentration of 4 × 109cfu/mL or more.
Preferably, in the step (2),
inoculating photosynthetic bacteria in fermentation culture medium, culturing at 32-35 deg.C for 7d in constant temperature incubator with yellow fluorescent lamp, shaking and mixing uniformly every 18-24h during culture, and maintaining brightness at 2500lm/m of 2000-2。
Preferably, in the step (2),
the frequency of shaking and mixing is 30 times/min.
Preferably, in the step (3),
inoculating the nitrogen-fixing bacillus seed liquid, the bacillus subtilis seed liquid, the yeast seed liquid and the lactic acid bacteria seed liquid in the photosynthetic bacteria suspension according to the mass percentages of 6-8%, 2-5%, 8-10% and 6-8% respectively to ensure that the thallus concentration in the photosynthetic bacteria suspension is 4 multiplied by 109-9×109cfu/mL。
Preferably, in the step (3),
according to tomato juice before fermentation: the mass ratio of the glucose is (15-20): (20-30) supplementing glucose into the photosynthetic bacteria suspension;
the temperature is controlled at 32-35 deg.C, the stirring speed is 30-40rpm, and the culture is carried out for 48-72 h.
And during fermentation, low-speed stirring is carried out, so that on one hand, aerobic fermentation conditions are provided, on the other hand, a large amount of heat generated in the fermentation process is dissipated in time, and the stability of the fermentation temperature is ensured.
Preferably, in step (3)
The culture medium for the propagation of the nitrogen-fixing paenibacillus comprises the following raw materials in parts by weight:
25-30 parts of glucose, 8-12 parts of corn flour, 8-13 parts of soybean meal, 10-15 parts of yeast extract, 0.01-0.05 part of sodium chloride, 5-10 parts of calcium carbonate and K2HPO40.5-1 part and 800-1200 parts of water;
the culture medium for expanding propagation of the bacillus subtilis comprises the following raw materials in parts by weight:
glucose 25-30 parts, corn flour 8-12 parts, soybean meal 8-13 parts, MgSO40.05-0.08 part of (NH)4)2SO40.1-0.3 part of K2HPO40.5-0.9 part and 800-1200 parts of water;
the culture medium for yeast propagation comprises the following raw materials in parts by weight:
10-15 parts of yeast extract, 10-15 parts of yolk, 15-20 parts of glucose, 10-15 parts of peptone and 1200 parts of water;
the culture medium for propagation of the lactic acid bacteria comprises the following raw materials in parts by weight:
20-25 parts of glucose, 3-6 parts of yeast extract, 10-15 parts of peptone, 1-4 parts of disodium hydrogen citrate, 0.5-1 part of magnesium sulfate and K2HPO41-4 parts and 800-1200 parts of water;
the propagation culture temperature of the nitrogen-fixing bacillus, the bacillus subtilis, the microzyme and the lactic acid bacteria is 32-35 ℃, the culture time is 2-3d, the stirring speed is 10-20rpm, and the air volume is 100 plus 150L/h.
The microbial deodorant prepared by the method is applied to deodorizing domestic garbage liquid or livestock excrement and urine, and the dosage of the microbial deodorant is 1-1.5kg/m3。
After the microbial deodorant is applied to a water body containing domestic garbage liquid or livestock excrement and urine, the water body property can be effectively changed, the breeding of substances such as harmful bacteria, nematodes and the like in the water body is reduced, and the quantity of beneficial bacteria and harmful bacteria in the water body is effectively balanced; efficiently absorbed SO2、H2S, and other gases with foul smell, inhibiting NH3And (4) volatilizing.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of a microbial deodorant comprises the following steps:
(1) preparing a fermentation medium
According to the mass ratio of the soybean sprouts to water of 1: 1.2, placing the soybean sprouts in water for decocting for 30min to obtain a base solution;
diluting the base solution 12 times (by mass) with water to obtain a diluted solution;
mixing tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate according to the weight ratio of 16: 10: 0.6: mixing at 0.05 mass ratio;
mixing the diluent with a mixed solution of tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate according to a ratio of 1: mixing at a mass ratio of 1.5, sterilizing, and making into fermentation culture medium.
(2) Fermentation of photosynthetic bacteria
Inoculating photosynthetic bacteria (sold in the market) in a fermentation culture medium according to the mass percent of 18%, placing the fermentation culture medium in a constant temperature incubator with a yellow fluorescent lamp, shaking and mixing uniformly clockwise when the temperature rises to 35 ℃, and then shaking and mixing uniformly every 20 hours, wherein the frequency is 30 times/min when shaking; during the culture process, the temperature is controlled at 32-35 deg.C, and the brightness is 2200lm/m2. After 7 days of culture, the thallus concentration in the obtained photosynthetic bacteria suspension is more than or equal to 4 multiplied by 109cfu/mL。
(3) Fermenting with composite bacteria
Respectively carrying out propagation on nitrogen-fixing bacillus, bacillus subtilis, saccharomycetes and lactic acid bacteria (all commercially available):
the culture medium for the propagation of the nitrogen-fixing paenibacillus comprises the following raw materials in parts by weight:
25 parts of glucose, 10 parts of corn flour, 10 parts of soybean meal, 12 parts of yeast extract, 0.02 part of sodium chloride, 8 parts of calcium carbonate and K2HPO40.6 part and 1000 parts of water;
the culture medium for expanding propagation of the bacillus subtilis comprises the following raw materials in parts by weight:
25 parts of glucose, 10 parts of corn flour, 10 parts of soybean meal and MgSO40.06 part of (NH)4)2SO40.2 part of K2HPO40.8 part and 1000 parts of water;
the culture medium for yeast propagation comprises the following raw materials in parts by weight:
12 parts of yeast extract, 12 parts of egg yolk, 18 parts of glucose, 12 parts of peptone and 1000 parts of water;
the culture medium for propagation of the lactic acid bacteria comprises the following raw materials in parts by weight:
25 parts of glucose, 5 parts of yeast extract, 12 parts of peptone, 3 parts of disodium hydrogen citrate, 0.8 part of magnesium sulfate and K2HPO42 parts and 1000 parts of water;
the propagation culture temperature of the nitrogen-fixing bacillus, the bacillus subtilis, the microzyme and the lactic acid bacteria is 32-35 ℃, the culture time is 3d, the stirring speed is 15rpm, and the air volume is 120L/h.
Respectively inoculating the obtained azotobacteria seed liquid, bacillus subtilis seed liquid, yeast seed liquid and lactobacillus seed liquid in the photosynthetic bacteria suspension according to the mass percent of 7%, 3%, 9% and 7% to ensure that the thallus concentration in the photosynthetic bacteria suspension is about 6 multiplied by 109cfu/mL；
According to the tomato juice: the mass ratio of glucose is 1: 2, supplementing glucose into the photosynthetic bacteria suspension;
the temperature in the fermentation process is controlled to be 32-35 ℃, and the stirring speed is 35 rpm.
And fermenting for 3d to obtain the microbial deodorant.
Example 2
A preparation method of a microbial deodorant comprises the following steps:
(1) preparing a fermentation medium
According to the mass ratio of the soybean sprouts to water of 1: 1.1, placing the soybean sprouts in water for decocting for 30min to obtain a base solution;
diluting the base solution 10 times (by mass) with water to obtain a diluted solution;
mixing tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate according to the weight ratio of 15: 9: 0.5: mixing at 0.04 mass ratio;
mixing the diluent with a mixed solution of tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate according to a ratio of 1: 1.2, sterilizing and preparing the fermentation medium.
(2) Fermentation of photosynthetic bacteria
Photosynthetic bacteria (commercially available) were added in an amount of 15% by massInoculating the mixture in percentage into a fermentation medium, placing the mixture in a constant temperature incubator with a yellow fluorescent lamp, shaking clockwise to mix uniformly when the temperature rises to 35 ℃, and then shaking uniformly every 18 hours, wherein the frequency is 30 times/min; during the culture process, the temperature is controlled at 32-35 deg.C, and the brightness is 2000lm/m2. After 7 days of culture, the thallus concentration in the obtained photosynthetic bacteria suspension is more than or equal to 4 multiplied by 109cfu/mL。
(3) Fermenting with composite bacteria
Respectively carrying out propagation on nitrogen-fixing bacillus, bacillus subtilis, saccharomycetes and lactic acid bacteria (all commercially available):
the culture medium for the propagation of the nitrogen-fixing paenibacillus comprises the following raw materials in parts by weight:
25 parts of glucose, 10 parts of corn flour, 10 parts of soybean meal, 12 parts of yeast extract, 0.02 part of sodium chloride, 8 parts of calcium carbonate and K2HPO40.6 part and 1000 parts of water;
the culture medium for expanding propagation of the bacillus subtilis comprises the following raw materials in parts by weight:
25 parts of glucose, 10 parts of corn flour, 10 parts of soybean meal and MgSO40.06 part of (NH)4)2SO40.2 part of K2HPO40.8 part and 1000 parts of water;
the culture medium for yeast propagation comprises the following raw materials in parts by weight:
12 parts of yeast extract, 12 parts of egg yolk, 18 parts of glucose, 12 parts of peptone and 1000 parts of water;
the culture medium for propagation of the lactic acid bacteria comprises the following raw materials in parts by weight:
25 parts of glucose, 5 parts of yeast extract, 12 parts of peptone, 3 parts of disodium hydrogen citrate, 0.8 part of magnesium sulfate and K2HPO42 parts and 1000 parts of water;
the propagation culture temperature of the nitrogen-fixing bacillus, the bacillus subtilis, the microzyme and the lactic acid bacteria is 32-35 ℃, the culture time is 2d, the stirring speed is 10rpm, and the air volume is 100L/h.
Respectively performing propagation on the obtained nitrogen-fixing bacillus seed solution, bacillus subtilis seed solution, yeast seed solution and lactobacillus seed solution according to the ratio of 6%, 2%, 8% and 6%Inoculating the mixture into photosynthetic bacteria suspension by mass percent to ensure that the thallus concentration in the photosynthetic bacteria suspension is about 5 multiplied by 109cfu/mL；
According to the tomato juice: supplementing glucose into the photosynthetic bacteria suspension with a glucose mass ratio of 3: 4;
the temperature in the fermentation process is controlled to be 32-35 ℃, and the stirring speed is 30 rpm.
And fermenting for 3d to obtain the microbial deodorant.
Example 3
A preparation method of a microbial deodorant comprises the following steps:
(1) preparing a fermentation medium
According to the mass ratio of the soybean sprouts to water of 1: 1.4, putting the soybean sprouts into water and decocting for 30min to obtain a base solution;
diluting the base solution 15 times (by mass) with water to obtain a diluted solution;
mixing tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate according to the weight ratio of 20: 12: 0.8: 0.07 mass ratio;
mixing the diluent with a mixed solution of tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate according to a ratio of 1: mixing at a mass ratio of 1.5, sterilizing, and making into fermentation culture medium.
(2) Fermentation of photosynthetic bacteria
Inoculating photosynthetic bacteria (sold in the market) into a fermentation culture medium according to the mass percent of 20%, placing the fermentation culture medium into a constant-temperature incubator with a yellow fluorescent lamp, shaking and mixing the photosynthetic bacteria and the culture medium uniformly clockwise when the temperature rises to 35 ℃, and then shaking and mixing the photosynthetic bacteria and the culture medium uniformly every 24 hours, wherein the frequency of shaking is 30 times/min; during the culture process, the temperature is controlled at 32-35 deg.C, and the brightness is 2500lm/m2. After 7 days of culture, the thallus concentration in the obtained photosynthetic bacteria suspension is more than or equal to 4 multiplied by 109cfu/mL。
(3) Fermenting with composite bacteria
Respectively carrying out propagation on nitrogen-fixing bacillus, bacillus subtilis, saccharomycetes and lactic acid bacteria (all commercially available):
the culture medium for the propagation of the nitrogen-fixing paenibacillus comprises the following raw materials in parts by weight:
25 parts of glucose, 10 parts of corn flour and 10 parts of soybean meal12 parts of yeast extract, 0.02 part of sodium chloride, 8 parts of calcium carbonate and K2HPO40.6 part and 1000 parts of water;
the culture medium for expanding propagation of the bacillus subtilis comprises the following raw materials in parts by weight:
25 parts of glucose, 10 parts of corn flour, 10 parts of soybean meal and MgSO40.06 part of (NH)4)2SO40.2 part of K2HPO40.8 part and 1000 parts of water;
the culture medium for yeast propagation comprises the following raw materials in parts by weight:
12 parts of yeast extract, 12 parts of egg yolk, 18 parts of glucose, 12 parts of peptone and 1000 parts of water;
the culture medium for propagation of the lactic acid bacteria comprises the following raw materials in parts by weight:
25 parts of glucose, 5 parts of yeast extract, 12 parts of peptone, 3 parts of disodium hydrogen citrate, 0.8 part of magnesium sulfate and K2HPO42 parts and 1000 parts of water;
the propagation culture temperature of the nitrogen-fixing bacillus, the bacillus subtilis, the microzyme and the lactic acid bacteria is 32-35 ℃, the culture time is 3d, the stirring speed is 20rpm, and the air volume is 150L/h.
Respectively inoculating 8%, 5%, 10% and 8% of Bacillus azotobacteria seed solution, Bacillus subtilis seed solution, yeast seed solution and lactobacillus seed solution obtained by propagation into photosynthetic bacteria suspension to make the thallus concentration in photosynthetic bacteria suspension about 9 × 109cfu/mL；
According to the tomato juice: the mass ratio of glucose is 2: 3, supplementing glucose into the photosynthetic bacteria suspension;
the temperature in the fermentation process is controlled to be 32-35 ℃, and the stirring speed is 40 rpm.
And fermenting for 3d to obtain the microbial deodorant.
Example 4
The microbial deodorant prepared in example 1 was used to treat domestic waste liquid and livestock feces and urine in an amount of 1.2kg/m3(ii) a Setting control group and untreated blank group, wherein the control group uses commercially available photosynthetic bacteria and solidAnd (3) directly mixing bacillus azotobacteria, bacillus subtilis, saccharomycetes and lactic acid bacteria according to the concentration of each bacterium in the microbial deodorant in the embodiment 1 to obtain the microbial agent.
After 14 days of treatment, the SO of each group was determined2、H2S、NH3Concentration, clearance relative to the blank was calculated.
TABLE 1
The general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A preparation method of a microbial deodorant is characterized by comprising the following steps:
(1) preparing a fermentation medium:
decocting soybean sprout in water to obtain base solution;
diluting the base solution, and mixing with tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate to obtain a fermentation medium;
(2) photosynthetic bacteria fermentation:
inoculating photosynthetic bacteria into a fermentation culture medium, and culturing to obtain photosynthetic bacteria suspension;
(3) fermenting by using compound bacteria:
respectively carrying out propagation on nitrogen-fixing bacillus, bacillus subtilis, saccharomycetes and lactic acid bacteria;
inoculating the bacillus azotobacteria seed liquid, the bacillus subtilis seed liquid, the yeast seed liquid and the lactobacillus seed liquid which are obtained by propagation into the photosynthetic bacteria suspension, supplementing a carbon source, and fermenting to obtain the microbial deodorant.
2. The method for producing a microbial deodorant according to claim 1,
in the step (1), the step (c),
the mass ratio of the soybean sprouts to water used in the soybean sprout decoction is 1: (1.1-1.4);
decocting and boiling for more than 30 min.
3. The method for producing a microbial deodorant according to claim 1,
in the step (1), the step (c),
the base liquid is prepared according to the following steps of 1: (10-15) diluting in a mass ratio, wherein the mass ratio of the obtained diluent to other components in the fermentation medium is 1: (1.2-1.5);
the mass ratio of tomato juice, sodium chloride, sodium sulfate and dipotassium hydrogen phosphate is (15-20): (9-12): (0.5-0.8): (0.04-0.07).
4. The method for producing a microbial deodorant according to claim 1,
in the step (2),
inoculating photosynthetic bacteria in fermentation culture medium at 15-20 wt%, and culturing to obtain photosynthetic bacteria suspension with thallus concentration of 4 × 109cfu/mL or more.
5. The method for producing a microbial deodorant according to claim 1,
in the step (2),
inoculating photosynthetic bacteria in fermentation culture medium, culturing at 32-35 deg.C for 7d in constant temperature incubator with yellow fluorescent lamp, shaking and mixing uniformly every 18-24h during culture, and maintaining brightness at 2500lm/m of 2000-2。
6. The method for producing a microbial deodorant according to claim 5,
in the step (2),
the frequency of shaking and mixing is 30 times/min.
7. The method for producing a microbial deodorant according to claim 1,
in the step (3), the step (c),
inoculating the nitrogen-fixing bacillus seed liquid, the bacillus subtilis seed liquid, the yeast seed liquid and the lactic acid bacteria seed liquid in the photosynthetic bacteria suspension according to the mass percentages of 6-8%, 2-5%, 8-10% and 6-8% respectively to ensure that the thallus concentration in the photosynthetic bacteria suspension is 4 multiplied by 109-9×109cfu/mL。
8. The method for producing a microbial deodorant according to claim 1,
in the step (3), the step (c),
according to tomato juice before fermentation: the mass ratio of the glucose is (15-20): (20-30) supplementing glucose into the photosynthetic bacteria suspension;
the temperature is controlled at 32-35 deg.C, the stirring speed is 30-40rpm, and the culture is carried out for 48-72 h.
9. The method for producing a microbial deodorant according to claim 1,
in the step (3)
The culture medium for the propagation of the nitrogen-fixing paenibacillus comprises the following raw materials in parts by weight:
25-30 parts of glucose, 8-12 parts of corn flour, 8-13 parts of soybean meal, 10-15 parts of yeast extract, 0.01-0.05 part of sodium chloride, 5-10 parts of calcium carbonate and K2HPO40.5-1 part and 800-1200 parts of water;
the culture medium for expanding propagation of the bacillus subtilis comprises the following raw materials in parts by weight:
25-30 parts of glucose, 8-12 parts of corn flour, 8-13 parts of soybean meal and MgSO40.05-0.08 part of (NH)4)2SO40.1-0.3 part of K2HPO40.5-0.9 part and 800-1200 parts of water;
the culture medium for yeast propagation comprises the following raw materials in parts by weight:
10-15 parts of yeast extract, 10-15 parts of yolk, 15-20 parts of glucose, 10-15 parts of peptone and 1200 parts of water;
the culture medium for propagation of the lactic acid bacteria comprises the following raw materials in parts by weight:
20-25 parts of glucose, 3-6 parts of yeast extract, 10-15 parts of peptone, 1-4 parts of disodium hydrogen citrate, 0.5-1 part of magnesium sulfate and K2HPO41-4 parts and 800-1200 parts of water;
the propagation culture temperature of the nitrogen-fixing bacillus, the bacillus subtilis, the microzyme and the lactic acid bacteria is 32-35 ℃, the culture time is 2-3d, the stirring speed is 10-20rpm, and the air volume is 100 plus 150L/h.
10. Use of a microbial deodorant prepared by the process of any one of claims 1 to 9 for deodorizing a domestic waste liquid or feces and urine of livestock,
the dosage of the microbial deodorant is 1-1.5kg/m3。
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| CN1861199A (en) * | 2005-05-12 | 2006-11-15 | 中国农业大学 | Biological deodorization and purification agent, and its use |
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