CN108065037A - Microbial fermentation prepares the method and functional feed of functional feed - Google Patents
Microbial fermentation prepares the method and functional feed of functional feed Download PDFInfo
- Publication number
- CN108065037A CN108065037A CN201711471049.5A CN201711471049A CN108065037A CN 108065037 A CN108065037 A CN 108065037A CN 201711471049 A CN201711471049 A CN 201711471049A CN 108065037 A CN108065037 A CN 108065037A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- functional feed
- solid medium
- dha
- time
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to the methods and functional feed that a kind of microbial fermentation prepares functional feed, belong to field of fermentation engineering.Preparation method includes：Microorganism fungus kind is fermented in fluid nutrient medium for the first time after 36 48h, be then seeded in solid medium second of 100 140h of fermentation.Microorganism fungus kind is the microorganism with production DHA abilities.This method is simple, take solid-liquid fermentation combined process, pass through the content and protein content of DHA in the effective control function feed of fermentation period, it is of low cost, low energy consumption, equipment damage is small, without waste water discarded object generate, the treatment and finishing section that ferments is simple, solves the problems, such as that fermentation thalli is directly granulated difficulty.Functional feed in the present invention contains abundant DHA and protein, and nutritive value is high, can improve the production performance of animal and the quality of animal product.
Description
Technical field
The present invention relates to field of fermentation engineering, and more particularly to a kind of microbial fermentation prepare functional feed method and
Functional feed.
Background technology
Docosahexaenoic acid (DHA) is a kind of long-chain polyunsaturated fatty acid with important physiological function, can be promoted
Brain and visual acuity improve intelligence, therefore make an addition to extensively in baby milk powder.In addition, DHA, which has, prevents and treats painstaking effort
Pipe disease prevents senile dementia, inhibits the physiological functions such as cell carcinogenesis, thus is widely used in food industry.
The study found that schizochytrium limacinum can largely accumulate DHA, the more other microalgaes of DHA content are high in algae oil.In addition, there is it
Its a variety of microorganism fungus kind that can be used to produce DHA.But the quasi-microorganism is mostly fermented using neat liquid, is needed not in fermentation process
Open close gas and stirring are to ensure dissolved oxygen, and liquid fermentation water content is up to more than 80%, and drying process energy consumption is higher, liquid fermentation
The DHA of output belongs to polyunsaturated fatty acid and is oxidized easily, it is necessary to further embedding treatment.
It therefore, need to be to being improved in the prior art using the method for micro-organisms DHA.
The content of the invention
The first object of the present invention is to provide a kind of method that microbial fermentation prepares functional feed, this method letter
It is single, solid-liquid fermentation combined process is taken, is contained by the content and protein of DHA in the effective control function feed of fermentation period
Amount, it is of low cost, low energy consumption, equipment damage is small, without waste water discarded object generate, fermentation treatment and finishing section it is simple, solve fermentation
Thalline is directly granulated the problem of difficulty.
The second object of the present invention is to provide a kind of functional feed, which contains abundant DHA and protein, battalion
It is high to support value, the production performance of animal and the quality of animal product can be improved.
The present invention is solved its technical problem and is realized using following technical scheme：
The present invention proposes a kind of method that microbial fermentation prepares functional feed, comprises the following steps：By microbial bacteria
The 36-48h that ferments for the first time in fluid nutrient medium is planted, is then seeded in solid medium second of fermentation 100-140h.
Microorganism fungus kind is the microorganism of the ability with production DHA.
It is preferred that microorganism fungus kind includes any one in schizochytrium limacinum, thraustochytriale, dino flagellate and saccharomycete.
The ingredient of fluid nutrient medium includes glucose, sodium glutamate, yeast extract and inorganic salts.
The ingredient of solid medium includes protein matter, inorganic salts and vitamin.
The present invention also proposes a kind of functional feed, which can be used above-mentioned preparation method and be prepared.
Microbial fermentation prepares the method for functional feed and the beneficial effect of functional feed in present pre-ferred embodiments
Fruit is：
By giving schizochytrium limacinum preferable growing environment first with liquid fermentation, when its cell concentration is higher, carry out
Sterile enrichment method is simultaneously transferred in the mode of solid medium relaying supervention ferment, can be made in fermentation process without constantly ventilating and stirring
It mixes, avoids the problems such as complicated for operation and energy consumption cost caused by the neat liquid fermentation used in conventional fermentation process is high.
Protein matter is utilized mainly as nitrogen source for schizochytrium limacinum, and selected protein matter contains abundant protein,
Raw material sources are relatively easy and cost is relatively low.
The functional feed obtained by the above method contains abundant DHA and protein, and nutritive value is high, can improve animal
The nutritive value of diet improves the production performance of animal and the quality of animal product.
Specific embodiment
It, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Prepared by the method for functional feed to the microbial fermentation of the embodiment of the present invention below and functional feed has
Body explanation.
An embodiment of the present invention provides a kind of method that functional feed is prepared using microbial fermentation, such as can include
Following steps：Microorganism fungus kind in fluid nutrient medium is fermented for the first time, second is then seeded in solid medium and sends out
Ferment.By giving schizochytrium limacinum preferable growing environment first with liquid fermentation, when its cell concentration is higher, carry out sterile dense
Contracting is enriched with and transfers relays supervention ferment in solid medium.As a result, without constantly ventilating and stirring in fermentation process, so as to avoid
The problems such as complicated for operation and energy consumption cost caused by the neat liquid fermentation used in conventional fermentation process is high.
Mentioned microorganism strain is the microorganism of the ability with production DHA, optionally, such as can be fragmentation pot
Any one in bacterium, thraustochytriale, dino flagellate and saccharomycete.Optionally, the ingredient of aforesaid liquid culture medium is for example
It can include glucose, inorganic salts, sodium glutamate and/or yeast extract.Wherein, glucose, sodium glutamate, yeast extract and nothing
The mass percent of machine salt in liquid medium can for example be followed successively by 5-7wt%, 2-4wt%, 0.8-1wt% and 3-
5wt% is preferably 6wt%, 3wt%, 0.9wt% and 4wt%.
It is preferred that inorganic salt can such as include the phosphate of 0.5-0.7wt%, the sulfate of 0.7-0.9wt%, 1.5-
The sodium salt of 2.5wt% and the calcium chloride of 0.02-0.04wt% etc..Preferably, the potassium dihydrogen phosphate of inorganic salts including 0.6wt%,
The magnesium sulfate of 0.8%wt%, the sodium chloride of 2wt%, the sodium sulphate of the calcium chloride of 0.03wt% and 0.44wt%.
Optionally, the ingredient of above-mentioned solid medium can for example include protein matter, inorganic salts and vitamin.
Preferably, protein matter can for example include corn, wheat and soya bean and its derivative and processing byproduct, of the invention real
It applies in example, corn can be by taking cornstarch and Dried Corn Steep Liquor Powder as an example, and wheat can be by taking wheat bran as an example, and soya bean can be with
Exemplified by soybean cake powder.Such protein matter is utilized mainly as nitrogen source for schizochytrium limacinum, containing abundant protein.This
Outside, such protein matter can preferably increase the porosity between culture medium, make to have centainly between each material component
Hole, to improve the biography oxygen transfer effect in incubation.
In addition, protein matter can also include relatively easy raw material sources and lower-cost xylose residue, spirulina powder
At least one of with feather meal.
It is preferred that it is 10-14 that solid medium, which can contain mass ratio,：38-42：8-12：8-12：3-5：The corn of 3-5
Starch, wheat bran, soybean cake powder, Dried Corn Steep Liquor Powder, inorganic salts and vitamin.More preferably, cornstarch, wheat bran, Huang
Beancake powder, Dried Corn Steep Liquor Powder, the mass ratio of inorganic salts and vitamin are 12：40：10：10：4：4.
Wherein, the species of inorganic salts can refer to fluid nutrient medium, and vitamin can for example include the thiamines of 0.8-1.2wt%
The cyanocobalamin of element, the calcium pantothenate of 0.2-0.4wt%, the biotin of 0.004-0.008wt% and 0.01-0.02wt%.It is preferred that
Ground, vitamin include the thiamine of 1.0wt%, the calcium pantothenate of 0.3wt%, the biotin of 0.006wt% and 0.015wt%
Cyanocobalamin.
In order to which the schizochytrium limacinum in solid medium is given to provide good growth and yeasting, the embodiment of the present invention is solid
It is 10-14 to be additionally added in body culture medium with cornstarch mass ratio：The water of 18-22, to obtain fluffy nutrient growth environment,
Avoid solid medium excessively dry and strong.Be inoculated with schizochytrium limacinum before, can also by the ingredient of mixed solid medium in
Boiling 10-20min under conditions of 95-100 DEG C so that the particulate component in solid medium is uniformly dispersed, is more conducively trained
Support the growth of the schizochytrium limacinum of different position in base.High-temperature sterilization at least 20min after boiling.
Schizochytrium limacinum is inoculated in fluid nutrient medium, ferment 36-48h for the first time under conditions of 26-30 DEG C.
Preferably, the temperature of fermentation is 27-29 DEG C for the first time, is 28 DEG C more preferably.Specifically, the temperature fermented for the first time
Degree can be 26 DEG C, 26.5 DEG C, 27 DEG C, 27.5 DEG C, 28 DEG C, 28.5 DEG C, 29 DEG C, 29.5 DEG C and 30 DEG C.
Preferably, the time of fermentation is 40-44h for the first time, is 42h more preferably.Specifically, the time fermented for the first time
Can be 36h, 38h, 40h, 42h, 44h, 46h and 48h.
The substance centrifugal concentrating of gained after first time is fermented collects the thalline after concentration and transfers in solid medium,
Second of fermentation 100-140h under conditions of 26-30 DEG C.
Preferably, the temperature of second of fermentation is also 27-29 DEG C, is 28 DEG C more preferably.Specifically, ferment for the second time
Temperature or 26 DEG C, 26.5 DEG C, 27 DEG C, 27.5 DEG C, 28 DEG C, 28.5 DEG C, 29 DEG C, 29.5 DEG C and 30 DEG C.
Preferably, the time of second of fermentation is 110-130h, is 120h more preferably.Specifically, for the first time fermentation when
Between can be 110h, 115h, 120h, 125h, 130h, 135h and 140h.
Optionally, the inoculum concentration of thalline can be for example the 10-30wt% of solid medium in solid medium, excellent
Elect 15-25wt% as, more excellent is 20wt%.It is inoculated with by above-mentioned preferred inoculum concentration, solid medium can be made to reach most with thalline
It is excessive or very few cause ferment effect and the not good enough feelings of tunning to avoid the occurrence of the nutriment needed for thalline fermentation for good balance
Condition.
It is preferred that stir solid medium once every 10-14h in second of fermentation process, stirring every time for example can be with
Continue 5-10min.Specifically, stirring every time can carry out in the following way：In sterile room with it is sterile shovel and it is sterile
The method that spreading rod is combined is stirred, and first with the sterile dynamic culture medium of shovel shovel, then carries out even spread with spreading rod, back and forth
2-3 bouts are stirred, stir 5-10min altogether, it should be noted that, ensure during stirring sterile.
By being stirred in second of fermentation process to solid medium, culture medium conglomeration can not only can be prevented,
It can also come into full contact with thalline and nutriment, reach in fluffy environment namely solid medium and accommodate certain sky
Gas and water form the environment for the suitable schizochytrium limacinum growth and breeding being made of solid, liquid, gas three-phase, and beneficial to dissolved oxygen.
Further, sterile water, optionally, nothing are supplemented in the embodiment of the present invention into solid medium during stirring
The magnitude of recruitment of bacterium water be solid medium 5-10wt%, such as can be 5wt%, 6wt%, 7wt%, 8wt%, 9wt% and
10wt%.It is preferred that different fermentations period rate of water make-up is different, because the earlier fermentation time is shorter, rate of water make-up can lack relatively, hair
Time ferment middle and later periods is long and moisture evaporation is more, therefore the fermentation period can enlarged relative rate of water make-up.By being mended during stirring
Sterile water is filled, on the one hand can aid in preventing culture medium conglomeration, on the other hand can be beneficial to mass transfer media of the water as solid fermentation,
Reach three kinds of states of solid, liquid, gas in fermentation to balance each other, to obtain good yeasting, enable schizochytrium limacinum preferable
Growth.
The culture medium and thalline obtained through the above method is respectively provided with the viscosity and surface state for meeting prilling requirement, from
And it solves in the prior art only through Wastewater treating is difficult, degree of drying is low, makes existing for the thalline obtained by neat liquid fermentation
The defects of grain difficulty and thalline cannot recycle, while also supplement the nutritional ingredient of high protein.
In consideration of it, it in the industrial production, will directly can ferment through second in fermentation process provided in an embodiment of the present invention
The solid medium of gained is dried with thalline, crushes and is granulated, and obtains making the functional production rich in DHA and high protein
Object.
Dry and crushing can carry out in microwave dryer, dry to substance to be dried without apparent moisture and in dried powder
Shape.It is preferred that the drying power in drying process is not less than 5kw, drying temperature is 90-110 DEG C, drying time 0.5-
2h.Index is granulated according to feed common index.
After measured, the functional product being prepared by above-mentioned preparation method has following characteristic：DHA content is 8wt%-
30wt%, protein content 30wt%-80wt%, crude fiber content are less than 10wt%.It is preferred that above-mentioned functional product
DHA content is 10wt%-20wt%, protein content 40wt%-60wt%, crude fiber content are less than 5wt%.
Further, the granularity of above-mentioned functional product is 0.8-6mm, moisture is no more than 10wt%, carbon-nitrogen ratio
For (15-30)：1.
Optionally, the functional feed in the embodiment of the present invention can directly be above-mentioned functional product, can also be through upper
Functional product is stated to be obtained by mixing with normal diet.
Specifically, above-mentioned functional feed can be animal feed or marine products feed to be applied to supporting for animal or marine products
It grows.By above-mentioned animal feed or marine products feed applications in the cultivation of animal or marine products, the diet battalion of animal or marine products can be improved
Value is supported, improves the production performance and product quality of animal or marine products.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
Schizochytrium limacinum is inoculated in fluid nutrient medium, ferment 48h for the first time under conditions of 26 DEG C, is then centrifuged for dense
The thalline after concentration is collected in contracting.Water is added in solid medium, the boiling 20min under conditions of 95 DEG C, sterilize 20min.It presses
Inoculum concentration transfers the thalline after concentration in solid medium for 10wt%, second of fermentation 140h under conditions of 26 DEG C.
The culture medium of second of fermentation gained is 5kw, is done in the microwave dryer that drying temperature is 90 DEG C with thalline in dry power
Dry crushing 2h, is then granulated, obtains functional feed.
Solid medium is stirred every 10h once, 5min can for example be continued every time by stirring in second of fermentation.It stirred
Sterile water is supplemented into solid medium by the 5wt% of solid medium in journey.
Wherein, fluid nutrient medium contains glucose, sodium glutamate, the yeast leaching of 5wt%, 2wt%, 0.8wt% and 3wt%
Cream and inorganic salts, inorganic salts contain the potassium dihydrogen phosphate of 0.5wt%, the magnesium sulfate of 0.7wt%, 1.5wt% sodium chloride,
The calcium chloride of 0.02wt% and the sodium sulphate of 0.40wt%.
Solid medium contains mass ratio for 10：38：8：8：3：3 corn, wheat, soya bean, Dried Corn Steep Liquor Powder, inorganic salts
And vitamin.Inorganic salts are identical with used in fluid nutrient medium.Vitamin contains the thiamine of 0.8wt%, the pantothenic acid of 0.2wt%
The cyanocobalamin of calcium, the biotin of 0.004wt% and 0.01wt%.Water added in solid medium and contained jade before boiling
The mass ratio of rice starch is 10：18.
Embodiment 2
Schizochytrium limacinum is inoculated in fluid nutrient medium, ferment 36h for the first time under conditions of 30 DEG C, is then centrifuged for dense
The thalline after concentration is collected in contracting.Water is added in solid medium, the boiling 10min under conditions of 100 DEG C, sterilize 30min.
The thalline after concentration is transferred in solid medium for 30wt% by inoculum concentration, is fermented for second under conditions of 30 DEG C
100h.Will second fermentation gained culture medium and thalline dry power be 10kw, the microwave drying that drying temperature is 110 DEG C
Drying and crushing 0.5h in machine, is then granulated, and obtains functional product.Above-mentioned functional product with normal diet is mixed, is obtained
Functional feed.
Solid medium is stirred every 14h once, 10min can for example be continued every time by stirring in second of fermentation.It stirs
Sterile water is supplemented into solid medium by the 10wt% of solid medium in the process.
Wherein, fluid nutrient medium contains the glucose, sodium glutamate, yeast extract of 7wt%, 4wt%, 1wt% and 5wt%
And inorganic salts, inorganic salts contain the potassium dihydrogen phosphate of 0.7wt%, the magnesium sulfate of 0.9wt%, 2.5wt% sodium chloride,
The calcium chloride of 0.04wt% and the sodium sulphate of 0.48wt%.
Solid medium contains mass ratio for 14：42：12：12：5：5 cornstarch, wheat bran, soybean cake powder, jade
Rice ＆ peanut milk dry powder, inorganic salts and vitamin.Inorganic salts are identical with used in fluid nutrient medium.Vitamin contains the thiamines of 1.2wt%
The cyanocobalamin of element, the calcium pantothenate of 0.4wt%, the biotin of 0.008wt% and 0.02wt%.Institute in solid medium before boiling
The mass ratio of the water added and contained cornstarch is 14：22.
Embodiment 3
Schizochytrium limacinum is inoculated in fluid nutrient medium, ferment 44h for the first time under conditions of 27 DEG C, is then centrifuged for dense
The thalline after concentration is collected in contracting.Water is added in solid medium, the boiling 15min under conditions of 98 DEG C, sterilize 30min.It presses
Inoculum concentration transfers the thalline after concentration in solid medium for 15wt%, second of fermentation 130h under conditions of 27 DEG C.
With thalline it is 12kw, in the microwave dryer that drying temperature is 100 DEG C in dry power by the culture medium of second fermentation gained
Drying and crushing 1h, is then granulated, and obtains functional product.Above-mentioned functional product with normal diet is mixed, obtains functionality
Feed.
Solid medium is stirred every 12h once, 7.5min can for example be continued every time by stirring in second of fermentation.It stirs
Sterile water is supplemented into solid medium by the 7wt% of solid medium in the process.
Wherein, fluid nutrient medium contains glucose, sodium glutamate, the yeast leaching of 6wt%, 3wt%, 0.9wt% and 4wt%
Cream and inorganic salts, inorganic salts contain the potassium dihydrogen phosphate of 0.6wt%, the magnesium sulfate of 0.8%wt%, 2wt% sodium chloride,
The calcium chloride of 0.03wt% and the sodium sulphate of 0.44wt%.
Solid medium contains mass ratio for 12：40：10：10：4：4 cornstarch, wheat bran, soybean cake powder, jade
Rice ＆ peanut milk dry powder, inorganic salts and vitamin.Inorganic salts are identical with used in fluid nutrient medium.Vitamin contains the thiamines of 1.0wt%
The cyanocobalamin of element, the calcium pantothenate of 0.3wt%, the biotin of 0.006wt% and 0.015wt%.Before boiling in solid medium
The mass ratio of added water and contained cornstarch is 12：20.
Embodiment 4
Schizochytrium limacinum is inoculated in fluid nutrient medium, ferment 40h for the first time under conditions of 29 DEG C, is then centrifuged for dense
The thalline after concentration is collected in contracting.Water is added in solid medium, the boiling 15min under conditions of 98 DEG C, sterilize 30min.It presses
Inoculum concentration transfers the thalline after concentration in solid medium for 25wt%, second of fermentation 110h under conditions of 29 DEG C.
With thalline it is 15kw, in the microwave dryer that drying temperature is 105 DEG C in dry power by the culture medium of second fermentation gained
Drying and crushing 1h, is then granulated, and obtains functional product.Above-mentioned functional product with normal diet is mixed, obtains functionality
Feed.
Solid medium is stirred every 12h once, 7.5min can for example be continued every time by stirring in second of fermentation.It stirs
Sterile water is supplemented into solid medium by the 8wt% of solid medium in the process.
Wherein, fluid nutrient medium contains glucose, sodium glutamate, the yeast leaching of 6wt%, 3wt%, 0.9wt% and 4wt%
Cream and inorganic salts, inorganic salts contain the potassium dihydrogen phosphate of 0.6wt%, the magnesium sulfate of 0.8%wt%, 2wt% sodium chloride,
The calcium chloride of 0.03wt% and the sodium sulphate of 0.44wt%.
Solid medium contains mass ratio for 12：40：10：10：4：4 cornstarch, wheat bran, soybean cake powder, jade
Rice ＆ peanut milk dry powder, inorganic salts and vitamin.Inorganic salts are identical with used in fluid nutrient medium.Vitamin contains the thiamines of 1.0wt%
The cyanocobalamin of element, the calcium pantothenate of 0.3wt%, the biotin of 0.006wt% and 0.015wt%.Before boiling in solid medium
The mass ratio of added water and contained cornstarch is 12：20.
Embodiment 5
Schizochytrium limacinum is inoculated in fluid nutrient medium, ferment 42h for the first time under conditions of 28 DEG C, is then centrifuged for dense
The thalline after concentration is collected in contracting.Water is added in solid medium, the boiling 15min under conditions of 98 DEG C, sterilize 30min.It presses
Inoculum concentration transfers the thalline after concentration in solid medium for 20wt%, second of fermentation 120h under conditions of 28 DEG C.
The culture medium of second of fermentation gained is 8kw, is done in the microwave dryer that drying temperature is 95 DEG C with thalline in dry power
Dry crushing 1.5h, is then granulated, obtains functional product.Above-mentioned functional product with normal diet is mixed, obtains functionality
Feed.
Solid medium is stirred every 12h once, 7.5min can for example be continued every time by stirring in second of fermentation.It stirs
Sterile water is supplemented into solid medium by the 7.5wt% of solid medium in the process.
Wherein, fluid nutrient medium contains glucose, sodium glutamate, the yeast leaching of 6wt%, 3wt%, 0.9wt% and 4wt%
Cream and inorganic salts, inorganic salts contain the potassium dihydrogen phosphate of 0.6wt%, the magnesium sulfate of 0.8%wt%, 2wt% sodium chloride,
The calcium chloride of 0.03wt% and the sodium sulphate of 0.44wt%.
Solid medium contains mass ratio for 12：40：10：10：4：4 cornstarch, wheat bran, soybean cake powder, jade
Rice ＆ peanut milk dry powder, inorganic salts and vitamin.Inorganic salts are identical with used in fluid nutrient medium.Vitamin contains the thiamines of 1.0wt%
The cyanocobalamin of element, the calcium pantothenate of 0.3wt%, the biotin of 0.006wt% and 0.015wt%.Before boiling in solid medium
The mass ratio of added water and contained cornstarch is 12：20.
Embodiment 6
The present embodiment is thraustochytriale difference lies in microorganism fungus kind used with embodiment 5.
Embodiment 7
The present embodiment is dino flagellate difference lies in microorganism fungus kind used with embodiment 5.
Embodiment 8
The present embodiment is saccharomycete difference lies in microorganism fungus kind used with embodiment 5.
Embodiment 9
Difference lies in the protein matters in solid medium also to contain xylose residue for the present embodiment and embodiment 5.Xylose
The mass ratio of slag and cornstarch is 4-6：10-14.
Embodiment 10
Difference lies in the protein matters in solid medium also to contain xylose residue and spiral for the present embodiment and embodiment 5
Algae powder.The mass ratio of xylose residue, spirulina powder and cornstarch is 2-3：1-2：10-14.
Embodiment 11
The present embodiment is with embodiment 5 difference lies in the protein matter in solid medium also containing xylose residue, spiral
Algae powder and feather meal.Xylose residue, spirulina powder, the mass ratio of feather meal and cornstarch are 1-2：1-2：1-2：10-14.
Embodiment 12
The present embodiment provides a kind of animal feed, which contains the functional feeding obtained by above-described embodiment 1-11
Material.
Embodiment 13
The present embodiment provides a kind of marine products feed, which contains the functional feeding obtained by above-described embodiment 1-11
Material.
Embodiment 14
The present embodiment provides a kind of baby milk, which contains the functional feeding obtained by above-described embodiment 1-11
Material.
Test example 1
It repeats to implement above-described embodiment 2-11, obtains enough functional feeds.By taking embodiment 5-8 as an example, setting pair
According to a group 1-4, control group 1-4 corresponds to embodiment 5-8, control group and embodiment difference lies in control group using existing pure respectively
Functional feed obtained by liquid fermentation mode (with reference to patent CN104312929B), comparative example 5-8 and control group 1-4
The content of middle DHA and protein (is accounted for by DHA/ protein in terms of the weight percent of functional feed), result such as Tables 1 and 2
It is shown.
The content (%) of 1 DHA of table and protein
The content (%) of 2 DHA of table and protein
It can be seen that the function as obtained by the functional feed obtained by embodiment 5-8 compared with control group 1-4 with reference to table 1 and table 2
The content of property feed, DHA and protein is considerably higher.Illustrating preparation method provided in an embodiment of the present invention, (solid-liquid ferments
Combined process) compared with the neat liquid zymotechnique of the prior art, it can ferment and generate more DHA and protein.
Also, embodiment 5 illustrates compared with the DHA of the functional feed obtained by embodiment 6-8 and the content higher of protein
The present invention program is particularly suited for schizochytrium limacinum in schizochytrium limacinum, thraustochytriale, dino flagellate and saccharomycete.
Test example 2
By taking the functional feed of embodiment 5 and control group 1 in test example 1 as an example, it is as shown in table 3 to compare its feed characteristics.
3 feed characteristics of table
As can be seen from Table 3, the more existing skill of preparation method (solid-liquid fermentation combined process) provided in an embodiment of the present invention
The functional feed that the neat liquid zymotechnique of art prepares gained has more preferably feed characteristics, and efficiently solves the current skill
Art field is granulated the problem of difficult.
In addition, being compared in the same way to embodiment 6-8 and control group 2-3, result equally shows that solid-liquid is sent out
Ferment combined process can obtain characteristic more preferably feed addictive compared with neat liquid zymotechnique, and not occur being granulated difficult ask
Topic.
Note：The DHA content scope of functional feed obtained by above-described embodiment 5-8 is 8wt%-30wt%, protein content
Scope is 30wt%-80wt%, crude fiber content is no more than 10wt%, size range 0.8-6mm, moisture and does not surpass
Cross 10wt%, carbon-nitrogen ratio scope is (15-30)：1.
Test example 3
By taking embodiment 12 as an example, the animal feed that embodiment 12 is provided replaces common feedstuffs to be used to feed pig, remaining is supported
It is constant to grow condition, cultivates 30 days, compares the Average weight increasing a day of pig after being cultivated with embodiment 12 compared with pig after being cultivated with common feedstuffs
Average weight increasing a day.In addition, the marine products feed that embodiment 13 provides is replaced common feedstuffs, for feeding silver carp, remaining cultivating condition is not
Become, cultivate 15 days, compare average daily increasing of the Average weight increasing a day compared with silver carp after being cultivated with common feedstuffs of silver carp after being cultivated with embodiment 13
Weight.The results are shown in Table 4 for it.
4 Average weight increasing a day of table
As can be seen from Table 4, raised using the animal feed provided in an embodiment of the present invention containing functional feed or marine products
Material cultivation pig or silver carp, the more common feed cultivation pig of energy or silver carp significantly improve its Average weight increasing a day namely improve feed conversion
Rate.
In conclusion the method that the microbial fermentation of the embodiment of the present invention prepares functional feed is simple, solid-liquid is taken
Ferment combined process, by the content and protein content of DHA in the effective control function feed of fermentation period, of low cost,
Low energy consumption, equipment damage is small, is generated without waste water discarded object, and fermentation treatment and finishing section is simple, solves fermentation thalli and is directly granulated
The problem of difficult.The functional feed obtained by the above method contains abundant DHA and protein, and nutritive value is high, can improve dynamic
The nutritive value of object diet improves the production performance of animal and the quality of animal product.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (11)
1. a kind of method that microbial fermentation prepares functional feed, which is characterized in that comprise the following steps：By microorganism fungus kind
Ferment 36-48h for the first time in fluid nutrient medium, is then seeded in solid medium second of fermentation 100-140h；
The microorganism fungus kind is the microorganism of the ability with production DHA；
The ingredient of the fluid nutrient medium includes glucose, inorganic salts, sodium glutamate and yeast extract；
The ingredient of the solid medium includes protein matter, inorganic salts and vitamin.
2. the method that microbial fermentation prepares functional feed according to claim 1, the microorganism fungus kind includes fragmentation
Any one in chytrid, thraustochytriale, dino flagellate and saccharomycete.
3. the method that microbial fermentation prepares functional feed according to claim 1, which is characterized in that for the first time fermentation with
The temperature of second of fermentation is 26-30 DEG C；
Preferably, the temperature of fermentation and second of fermentation is 27-29 DEG C for the first time；
More preferably, the temperature of fermentation and second of fermentation is 28 DEG C for the first time.
4. the method that microbial fermentation prepares functional feed according to claim 1, which is characterized in that fermented for the second time
The solid medium is stirred every 10-14h once, stir lasting 5-10min every time in journey.
5. the method that microbial fermentation prepares functional feed according to claim 4, which is characterized in that during stirring to
Sterile water is supplemented in the solid medium；
Preferably, the magnitude of recruitment of the sterile water is the 5-10wt% of the solid medium.
6. the method that microbial fermentation prepares functional feed according to claim 1, which is characterized in that inoculation micro- life
Before object strain, by the ingredient of the mixed solid medium under conditions of 95-100 DEG C boiling 10-20min.
7. the method that microbial fermentation prepares functional feed according to claim 1, which is characterized in that inoculum concentration is described
The 10-30wt% of solid medium；
Preferably, the inoculum concentration is the 15-25wt% of the solid medium；
More preferably, the inoculum concentration is the 20wt% of the solid medium.
8. the method that microbial fermentation prepares functional feed according to claim 1, which is characterized in that further include second
Solid medium obtained by secondary fermentation is dried with thalline, crushes and is granulated；
Preferably, dry power is not less than 5kw, and drying temperature is 90-110 DEG C, drying time 0.5-2h.
9. a kind of functional feed, which is characterized in that the DHA content of the functional feed is 8wt%-30wt%, albumen contains
It measures and is less than 10wt% for 30wt%-80wt%, crude fiber content.
10. functional feed according to claim 9, which is characterized in that the DHA content of the functional feed is
10wt%-20wt%, the protein content are 40wt%-60wt%, the crude fiber content is less than 5wt%.
11. according to any one of claim 9 or 10 functional feed, which is characterized in that the particle of the functional feed
It spends for 0.8-6mm, moisture is no more than 10wt%, carbon-nitrogen ratio is (15-30)：1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711471049.5A CN108065037A (en) | 2017-12-28 | 2017-12-28 | Microbial fermentation prepares the method and functional feed of functional feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711471049.5A CN108065037A (en) | 2017-12-28 | 2017-12-28 | Microbial fermentation prepares the method and functional feed of functional feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108065037A true CN108065037A (en) | 2018-05-25 |
Family
ID=62155928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711471049.5A Withdrawn CN108065037A (en) | 2017-12-28 | 2017-12-28 | Microbial fermentation prepares the method and functional feed of functional feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108065037A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527202A (en) * | 2018-12-29 | 2019-03-29 | 嘉必优生物技术（武汉）股份有限公司 | The method and feed of feed of the preparation rich in docosahexaenoic acid and bata-carotene |
| CN109527223A (en) * | 2018-12-29 | 2019-03-29 | 嘉必优生物技术（武汉）股份有限公司 | A kind of functional feed and preparation method thereof |
| CN109673815A (en) * | 2018-12-29 | 2019-04-26 | 嘉必优生物技术（武汉）股份有限公司 | Fermentation preparation is rich in the functional feed and preparation method of arachidonic acid and bata-carotene |
| CN110074256A (en) * | 2019-06-12 | 2019-08-02 | 瞿瀚鹏 | Improve the feed and preparation method thereof of ruminant milk fat rate |
| CN112772790A (en) * | 2020-12-31 | 2021-05-11 | 珠海市德海生物科技有限公司 | Raw fish feed additive and preparation method thereof |
| CN114009625A (en) * | 2021-10-18 | 2022-02-08 | 南京师范大学 | Aquaculture feed and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101500429A (en) * | 2006-08-09 | 2009-08-05 | 爱默思公司 | Methods of improving bone health and muscle health |
| US20130059028A1 (en) * | 2011-09-01 | 2013-03-07 | Julie Marie Foy | Compositions and Methods for Providing a Life-Stage-Appropriate Animal Diet |
| CN104212846A (en) * | 2014-08-28 | 2014-12-17 | 青岛海智源生命科技有限公司 | Method for producing DHA (docosahexenoic acid) by using cheap bean pulp as nitrogen source for fermenting schizochytrium |
| CN106333062A (en) * | 2016-08-31 | 2017-01-18 | 通威股份有限公司 | Method for increasing added values of raw materials of schizochytrium sp. solid-state fermented feed |
-
2017
- 2017-12-28 CN CN201711471049.5A patent/CN108065037A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101500429A (en) * | 2006-08-09 | 2009-08-05 | 爱默思公司 | Methods of improving bone health and muscle health |
| US20130059028A1 (en) * | 2011-09-01 | 2013-03-07 | Julie Marie Foy | Compositions and Methods for Providing a Life-Stage-Appropriate Animal Diet |
| CN104212846A (en) * | 2014-08-28 | 2014-12-17 | 青岛海智源生命科技有限公司 | Method for producing DHA (docosahexenoic acid) by using cheap bean pulp as nitrogen source for fermenting schizochytrium |
| CN106333062A (en) * | 2016-08-31 | 2017-01-18 | 通威股份有限公司 | Method for increasing added values of raw materials of schizochytrium sp. solid-state fermented feed |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527202A (en) * | 2018-12-29 | 2019-03-29 | 嘉必优生物技术（武汉）股份有限公司 | The method and feed of feed of the preparation rich in docosahexaenoic acid and bata-carotene |
| CN109527223A (en) * | 2018-12-29 | 2019-03-29 | 嘉必优生物技术（武汉）股份有限公司 | A kind of functional feed and preparation method thereof |
| CN109673815A (en) * | 2018-12-29 | 2019-04-26 | 嘉必优生物技术（武汉）股份有限公司 | Fermentation preparation is rich in the functional feed and preparation method of arachidonic acid and bata-carotene |
| CN109527202B (en) * | 2018-12-29 | 2022-06-10 | 嘉必优生物技术（武汉）股份有限公司 | Method for preparing feed rich in docosahexaenoic acid and beta carotene and feed |
| CN109673815B (en) * | 2018-12-29 | 2022-08-09 | 嘉必优生物技术（武汉）股份有限公司 | Functional feed rich in arachidonic acid and beta carotene prepared by fermentation and preparation method thereof |
| CN110074256A (en) * | 2019-06-12 | 2019-08-02 | 瞿瀚鹏 | Improve the feed and preparation method thereof of ruminant milk fat rate |
| CN112772790A (en) * | 2020-12-31 | 2021-05-11 | 珠海市德海生物科技有限公司 | Raw fish feed additive and preparation method thereof |
| CN114009625A (en) * | 2021-10-18 | 2022-02-08 | 南京师范大学 | Aquaculture feed and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108065037A (en) | Microbial fermentation prepares the method and functional feed of functional feed | |
| CN101107970B (en) | Bion cow concentrated feed and method for making same | |
| CN101215535B (en) | Solid fermentation process for preparing bacillus natto microecological preparation | |
| CN110150458A (en) | A method of forage protein is prepared using microbial fermentation black soldier flies larva | |
| CN102823726A (en) | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity | |
| CN102599351A (en) | Bio-enzyme and microorganism containing compound feed additive and preparation process thereof | |
| CN106889330A (en) | A kind of production method of nonreactive fermented feed for fattening pigs | |
| CN102823725A (en) | Method for producing biologic protein feed by using solid-state fermentation fiber dreg | |
| CN104311309A (en) | Indoor practical straw mushroom culture medium and preparation method thereof | |
| CN101632411B (en) | Method for preparing high-quality rapeseed protein containing conjugated linoleic acid by multi-strain fermentation | |
| CN109170151A (en) | The preparation method of fermentative feedstuff of microbe | |
| CN1171539C (en) | Nutrients additive for biological feed of livestock and fowls and its preparing process | |
| CN109678617A (en) | A kind of slow-release bio bacterial manure and preparation method based on stalk fibre | |
| CN109170150A (en) | The method for preparing feed using microorganism | |
| CN104762340A (en) | Method of converting nicotinonitrile into nicotinamide with immobilized cells | |
| CN106962643A (en) | A kind of production method of nonreactive grower pigs fermented feed | |
| CN101161809B (en) | Method for making ferment for agricultural byproducts | |
| CN103652387A (en) | Biological type milk cow concentrated feed and preparation method thereof | |
| CN111748493A (en) | Preparation method and application of compound microbial preparation | |
| CN1083671A (en) | De-poisoning method for cottonseed cake | |
| CN105918641A (en) | Soybean meal biological fermentation feed and preparation method thereof | |
| CN102669424A (en) | Microbe solid-state fermented biological feed and preparation method thereof | |
| CN103952356A (en) | Method for preparing phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure | |
| CN114525267B (en) | Method for improving keratinase production capacity of bacillus | |
| CN1074096A (en) | Rapeseed cake and cottonseed cake blended microbial detoxification method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180525 |
|
| WW01 | Invention patent application withdrawn after publication |