CN103652387A - Biological type milk cow concentrated feed and preparation method thereof - Google Patents
Biological type milk cow concentrated feed and preparation method thereof Download PDFInfo
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- CN103652387A CN103652387A CN201210354362.1A CN201210354362A CN103652387A CN 103652387 A CN103652387 A CN 103652387A CN 201210354362 A CN201210354362 A CN 201210354362A CN 103652387 A CN103652387 A CN 103652387A
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Abstract
The invention discloses a biological type milk cow concentrated feed and a preparation method thereof. The preparation method comprises the following steps: inoculating lactobacillus plantarum, bacillus subtilis and candida utilis strains in respective liquid seed culture medium respectively to prepare liquid strains; inoculating three liquid strains according to the weight ratio of 1:(0.8-1.2):(1.0-1.5) into a solid culture material composed of corn flour, bran, bean pulp and water, mixing uniformly, carrying out solid culture, culturing stage by stage at the temperature of 20-40 DEG C, culturing each stage for 20-26h, drying the culture material at a low temperature being less than 60 DEG C so as to control the moisture to be 10-14%, and then mixing the dried culture material with nine raw materials of corn, wheat bran, soybean cake and the like, so as to form the concentrated feed.
Description
Technical field
The invention belongs to field of fodder, be specifically related to a kind of Bion cow concentrated feed and preparation method thereof.
Background technology
Cow concentrated feed is in the market all that traditional formula forms, and seldom contains bio-additive composition, and adds the additive that some can produce medicament residue or cause environmental pollution, affects milk products edible safety.
The cow concentrated feed of available data report has: No. 200510054625, title " concentrated feed for keerxin dairy cattle " patent application be first trace element, vitamin, amino acid etc. to be made to premix, then be mixed and made into concentrated feed with beans, cottonseed, flax, DDG, DDGS, microprotein powder, corn protein powder etc.Owing to there is no sweat, and lack biological activity, cause feed conversion rate low, lack the microorganism compositions such as beneficial bacterium milk products quality is reduced.No. 200610002389, title " animal fermented concentrated feed and mixed feed and preparation method thereof and equipment " patent, its component mainly contains dregs of beans, peanut meal, corn protein powder, calcium phosphate, salt, trace element etc., preparation method, for first making bacterium liquid, is mixed bacterium liquid and is packed involution fermentation in fermentation bag into mentioned component.The fermentation of this patent is to ferment in involution bag, because fermentation condition is uncertain, will directly affect quality of the fodder.The production process of another kind of biological feedstuff and additive is mainly to adopt single culture fermentation technique, or after adopting different strain to ferment separately, then mix, make bion feed or additive.Can cause like this production cost to increase or the increase of production equipment input.As following two pieces of patent documentations: No. CN1296765, " biology feed additive and preparation method thereof ", by screening carry lactobacillus viridescens, the pure bacterium of lactobacillus coprophilus, saccharomycete, bacillus subtilis through once expansion cultivation, the secondary of culture medium, expand cultivate, with rice bran, wheat bran absorption bacterium liquid, heap fermentation, dry and reach bacterial classification quantity 8～1,000,000,000/gram biology feed additive.This patent is a kind of preparation method of additive patent.No. CN1659976, " Aditive of energetic biologic feedstuff for mitch cow ", this disclosure of the invention a kind of Aditive of energetic biologic feedstuff for mitch cow, it is that the parts by weight of each component are by the composition of probiotics, complex enzyme formulation, three kinds of compositions of fatty acid calcium: 10～60 parts of probioticses, 5～15 parts of complex enzyme formulations, 200～350 parts of fatty acid calciums.This patent is a kind of preparation method of biological forage for dairy cattle additive.The product of above-mentioned two pieces of documents is biology feed additives, not only cost of manufacture is high, and the synergy of selecting compatibility bacterial classification reaches perfect condition, there are data to show that additive adds type and cultivates bacterium material and compare the former with feedstuff mixed type the rate of giving milk of milk cow is had to certain influence.
Summary of the invention
The object of the invention is to overcome the various shortcomings and deficiencies of the said goods and method existence, a kind of Bion cow concentrated feed and ferment making technology thereof are provided, this technology adopts solid-liquid segmentation temperature-variable fermentation mode, can effectively solve the compound cultivation problem of many bacterial classifications.Adopt this technology to produce to contain to tolerate the probiotics of milk cow stomach particular surroundings and the compost of enzyme preparation, then according to dairy cow nutrition needs, coordinate other raw materials to make cow concentrated feed.
The technical solution adopted in the present invention is: selecting can mutual synergistic bacterial classification: bacillus, lactic acid bacteria, saccharomycete, first cultivate and make seed liquor separately, after the seed liquor of making is mixed in proportion, be linked in solid culture medium and ferment, by controlling temperature, first bacillus is grown, bacillus is a kind of aerobic bacteria, can in growth course, consume a large amount of oxygen, cause an anaerobic environment, so just for this anaerobic bacteria of lactic acid bacteria, cause a good growing environment, make the breeding of lactic acid bacteria raised growth, in lactobacter growth process, produce lactic acid, reduced the PH of compost, form a sour environment, so again for saccharomycete causes a good growing environment, impel Yeast Growth breeding.The breeding of every kind of bacterium all can cause for the breeding of another kind of bacterium a good environment.
The technical scheme of feed of the present invention is:
A) Lactobacillus plantarum, bacillus subtilis and candida utili bacterial classification are inoculated into respectively in liquid seed culture medium separately and make liquid spawn, three kinds of liquid spawns are inoculated in solid culture medium by 1: 0.8～1.2: 1.0～1.5 weight ratios;
B) solid culture medium is comprised of following weight parts:
30～50 parts of corn flour, 25～35 parts, wheat bran, 10～20 parts of dregs of beans, 40～50 parts, water;
C) the raw material composition concentrated feed of cultivation and fermentation dry compost and following weight parts;
30～50 parts of 8～13 parts of corns of compost
5～20 parts of 10～25 portions of soya-bean cakes of wheat bran
1.2～1.8 parts of 5～20 portions of salt of cottonseed cake
0.8～1.5 part of 1.5～2.3 parts of stone flour of soda
0.5～1.2 part, 0.5～1.2 part of calcium carbonate of bone meal.
The seed culture medium of three kinds of bacterial classifications of the present invention forms respectively, and the culture medium weight of Lactobacillus plantarum consists of: skimmed milk powder 3～5g, beef extract 3～5g, tomato juice 1～3g; Bacillus subtilis bacterium culture medium weight consists of: peptone 0.5～1.2g, yeast extract 0.5～1.2g, glucose 0.1～0.5g, manganese sulfate 0.02g, dipotassium hydrogen phosphate 0.02g, sodium chloride 0.1g; Candida utili bacterium culture medium weight consists of: maltose 1～3.0g, peptone 0.5～1.2g, yeast extract 0.5～1.0g.
Feed of the present invention is to prepare by the following method:
A) Lactobacillus plantarum, bacillus subtilis and candida utili bacterial classification are inoculated into respectively and in liquid seed culture medium separately, make liquid spawn;
B) three kinds of liquid spawns are inoculated in the solid culture medium being comprised of corn flour, wheat bran, dregs of beans and water and are mixed and carry out solid culture by 1: 0.8～1.2: 1.0～1.5 weight ratios, temperature is cultivated 20～26 hours at 28～32 ℃, cultivate 20～26 hours for 34～40 ℃, temperature is down to 26～32 ℃ again and is continued to cultivate 20～26 hours;
C) by compost in low temperature drying below 60 ℃, make moisture control 10～14%, more dried compost and corn, wheat bran, soya-bean cake, cottonseed cake, salt, soda, stone flour, bone meal, calcium carbonate be mixed and made into concentrated feed by above-mentioned batching.
The present invention compares tool with prior art with product and has the following advantages:
1, the present invention is because the bacterial classification unit that selects optimum synergistic to do solely cultivates, liquid is with mixing segmentation temperature-variable fermentation technique, can either effectively solve the compound cultivation problem of many bacterial classifications, can produce again the compost of the probiotics that contains tolerance milk cow stomach particular surroundings and enzyme preparation until feed, make the rate of giving milk of milk cow improve 8～22% than conventional method, butterfat percnetage improves 6～15%;
2, product of the present invention, containing profitable probliotics, can replace antibiotic etc additive, promotes milk cow intestinal microecology balance, and prevention of intestinal tract disease, improves immunity;
3, product, without any drug ingedient, solves milk source safety problem, produces green high quality milk nuisanceless, drug residue free;
4, contain various digestive ferments, improve feed quality, increase feed intake, improve feed digestibility, improve milk cow production performance;
5, purifying aquaculture environment.
The specific embodiment
Embodiment 1:
By the good bacillus subtilis Bacillus subtills of activation rejuvenation, Lactobacillus plantarum Lactobacillus plantarum, candida utili Candida utilis slant strains is inoculated into respectively in corresponding with it liquid seed culture medium, and Lactobacillus plantarum culture medium forms (weight composition): skimmed milk powder 5g, beef extract 5g, tomato juice 3g.Bacillus subtilis bacterium culture medium consists of (weight composition): peptone 1.0g, yeast extract 0.5g, glucose 0.5g, manganese sulfate 0.02g, dipotassium hydrogen phosphate 0.02g, sodium chloride 0.1g.Candida utili culture medium consists of (weight composition): maltose 3.0g, peptone 1.0g, yeast extract 1.0g.Liquid seeds is made in cultivation, Lactobacillus plantarum: bacillus subtilis: three kinds of bacterium liquid of candida utili mix by the weight ratio of 1: 1.2: 1.5 and are inoculated in solid culture medium, and the weight proportion of compost is: 45 parts of corn flour, 35 parts, wheat bran, 20 parts of dregs of beans, 50 parts, water.After kind of daughter bacteria liquid and compost are mixed, carry out solid culture.Initial temperature is 28～32 ℃, and after 24hr, temperature rises to 34～40 ℃, and after 24hr, temperature is reduced to 26～32 ℃, continues to cultivate after 24hr fermentation ends.In low temperature drying below 60 ℃, make moisture control 10～14% compost, make compost standby.
Embodiment 2:
Three kinds of bacterial classifications are inoculated into method that liquid seed culture medium inoculates solid culture medium with embodiment 1, and the weight proportion of solid culture medium is 40 parts of corn flour, 35 parts, wheat bran, and 15 parts of soya-bean cakes, 40 parts, water, fermented and cultured is made compost method with embodiment 1.
Embodiment 3:(raw material composition is weight portion)
Get the dried compost 10 of embodiment 1 or 2 preparations, corn 40, wheat bran 18, soya-bean cake 12.4, cottonseed cake 13, salt 1.65, soda 1.85, stone flour 1.15, bone meal 1.0, calcium carbonate 0.95 mixes, and makes Bion cow concentrated feed.
Embodiment 4:(raw material composition is weight portion)
Get the dried compost 10 of embodiment 1 or 2 preparations, corn 35, wheat bran 25, soya-bean cake 17, cottonseed cake 17, salt 1.55, soda 1.72, stone flour 1.03, bone meal 0.86, calcium carbonate 0.84 mixes, and makes Bion cow concentrated feed.
Embodiment 5:
By the dried compost 10 of embodiment 1 or 2 preparations, corn 45, wheat bran 15, soya-bean cake 15, cottonseed cake 18, salt 1.7%, soda 1.85, stone flour 1.23, bone meal 1.15, calcium carbonate 1.07 mixes, and makes Bion cow concentrated feed.
Claims (1)
1. Bion cow concentrated feed and preparation method thereof, it is characterized in that: a) Lactobacillus plantarum, bacillus subtilis and candida utili bacterial classification are inoculated into respectively and in liquid seed culture medium separately, make liquid spawn, three kinds of liquid spawn Lactobacillus plantarums: bacillus subtilis: candida utili bacterium is inoculated in solid culture medium by 1: 0.8～1.2: 1.0～1.5 weight ratios; The culture medium weight of described Lactobacillus plantarum consists of: skimmed milk powder 3～5g, beef extract 3～5g, tomato juice 1～3g; Bacillus subtilis bacterium culture medium weight consists of: peptone 0.5～1.2g, yeast extract 0.5～1.2g, glucose 0.1～0.5g, manganese sulfate 0.02g, dipotassium hydrogen phosphate 0.02g, sodium chloride 0.1g; Candida utili bacterium culture medium weight consists of: maltose 1～3.0g, peptone 0.5～1.2g, yeast extract 0.5～1.0g; B) solid culture medium is comprised of following weight parts: 30～50 parts of corn flour, 25～35 parts, wheat bran, 10～20 parts of dregs of beans, 40～50 parts, water; C) the raw material composition concentrated feed of cultivation and fermentation dry compost and following weight parts: 0.5～1.2 part, 0.5～1.2 part of calcium carbonate of 0.8～1.5 part of bone meal of 1.5～2.3 parts of stone flours of 1.2～1.8 parts of soda of 5～20 portions of salt of 5～20 portions of cottonseed cakes of 10～25 portions of soya-bean cakes of 30～50 parts of wheat bran of 8～13 parts of corns of compost; This Bion cow concentrated feed is prepared by the following method: a) Lactobacillus plantarum, bacillus subtilis and candida utili bacterial classification are inoculated into respectively and in liquid seed culture medium separately, make liquid spawn; B) three kinds of liquid spawns are inoculated in the solid culture medium being comprised of corn flour, wheat bran, dregs of beans and water and are mixed and carry out solid culture by 1: 0.8～1.2: 1.0～1.5 weight ratios, temperature is cultivated 20～26 hours at 28～32 ℃, cultivate 20～26 hours for 34～40 ℃, temperature is fallen 26～32 ℃ again and is continued to cultivate 20～26 hours; C) by compost in low temperature drying below 60 ℃, make moisture control 10～14%, more dried compost and corn, wheat bran, soya-bean cake, cottonseed cake, salt, soda, stone flour, bone meal, calcium carbonate be mixed and made into concentrated feed by above-mentioned batching.
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| CN201210354362.1A CN103652387A (en) | 2012-09-22 | 2012-09-22 | Biological type milk cow concentrated feed and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107361198A (en) * | 2017-07-18 | 2017-11-21 | 东北农业大学 | A kind of preparation method and applications of the biological enzymolysis fermentation peptide of promotion domestic animal lactation |
| CN108522793A (en) * | 2018-03-23 | 2018-09-14 | 哈尔滨伟平科技开发有限公司 | A kind of production method of cow feed additive |
| CN112322562A (en) * | 2020-12-25 | 2021-02-05 | 广西壮族自治区畜牧研究所 | Solid culture medium of bacillus, preparation method thereof and bacillus culture method |
-
2012
- 2012-09-22 CN CN201210354362.1A patent/CN103652387A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107361198A (en) * | 2017-07-18 | 2017-11-21 | 东北农业大学 | A kind of preparation method and applications of the biological enzymolysis fermentation peptide of promotion domestic animal lactation |
| CN108522793A (en) * | 2018-03-23 | 2018-09-14 | 哈尔滨伟平科技开发有限公司 | A kind of production method of cow feed additive |
| CN112322562A (en) * | 2020-12-25 | 2021-02-05 | 广西壮族自治区畜牧研究所 | Solid culture medium of bacillus, preparation method thereof and bacillus culture method |
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Application publication date: 20140326 |