CN105732195A - Composite microbial fertilizer for controlling soil-borne disease and preparation method thereof - Google Patents
Composite microbial fertilizer for controlling soil-borne disease and preparation method thereof Download PDFInfo
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- CN105732195A CN105732195A CN201610067288.3A CN201610067288A CN105732195A CN 105732195 A CN105732195 A CN 105732195A CN 201610067288 A CN201610067288 A CN 201610067288A CN 105732195 A CN105732195 A CN 105732195A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a composite microbial fertilizer for controlling soil-borne disease and a preparation method thereof. The composite microbial fertilizer for controlling soil-borne disease is prepared from the following raw materials in parts by weight: waste livestock/fowl feather, rice bran, dry manure, weathered coal, biochar, a composite microbial inoculant, humic acid, bentonite, potassium sodium tartrate, citric acid, copper sulfate and ferrous sulfate. The composite microbial inoculant is prepared from the following raw materials in parts by weight: Bacillus megaterium, Bacillus mucilagimosus krassilm and Paenibacillus sabinae. The composite microbial fertilizer contains abundant various microbes, and the composite microbial inoculant is added to increase the beneficial bacterium content, so that the composite microbial fertilizer becomes a microbe resource pool. After being applied by drip irrigation and spraying, the composite microbial fertilizer can adjust the phytoedaphon structure, enhance the soil microbe diversity, improve the microecological environment of the soil and reduce the biosphere of the phytopathogen, thereby achieving the goal of controlling soil-borne disease.
Description
Technical field
The present invention relates to technical field of fertilizers, specifically a kind of composite microbiological fertilizer preventing and treating soil-borne disease and preparation method thereof.
Background technology
China is as large agricultural country, and national economy is played vital effect by agricultural production, and fertilizer is the key factor affecting agricultural production yield and quality.But most fertilizer existing defects in nutritional labeling in the market, namely while improving yield, cause that some nutriment does not catch up with demand, or lay particular emphasis on stimulation plant growth, cause nutritional deficiency disease etc., the resistance causing crop is poor, and yield reduces and quality decline, it is impossible to meet the demand of present stage agricultural production.
Additionally, China is for many years due to the life-time service of chemical fertilizer, and fertilizer is not enough, and all kinds of nutrients ratios are lacked of proper care, cause farmland ecological environment, soil physico-chemical property and soil microflora to be subject to destruction in various degree, have impact on the safety of agricultural product to a certain extent.Biological organic fertilizer nutrient is comprehensive, it is possible to improvement soil, improves the soil compaction using chemical fertilizer to cause.Improve soil physical and chemical property, strengthen the ability of soil water-retaining, fertilizer conservation, fertilizer.Beneficial microbe in biological organic fertilizer forms mutual symbiosis Relationship With Proliferation with Soil Microorganism after entering soil, harmful bacteria is suppressed to grow and be converted into probiotics, interact, mutually promoting, play the synergism of colony, probiotics produces substantial amounts of metabolite in growth and breeding process, promote organic decomposition and inversion, multiple nutrients and pungent directly or indirectly can be provided for crop, promote and regulation and control plant growth, be a kind of environment-friendly type fertilizer.
Along with microbial technique application in agricultural, many function bacterium are also widely used in pesticide and fertilizer field, for realizing environmental protection, pollution-free and high-efficiency agriculture produces and provides effective method and solution route, therefore, the bio-feritlizer that many function bacterium participate in arises at the historic moment, but current bio-feritlizer is only as complementary fertilizer, the consumption of chemical fertilizer and pesticide is made to reduce, and the problem that can not fundamentally solve soil compaction.
Summary of the invention
It is an object of the invention to provide a kind of convenient sources, the composite microbiological fertilizer of prevented and treated soil-borne disease improving crop resistance and preparation method thereof, with the problem solving to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme:
A kind of composite microbiological fertilizer preventing and treating soil-borne disease, includes according to the raw material of weight portion: discarded poultry feather 80-90 part, Testa oryzae 40-50 part, argol 40-50 part, weathered coal 40-50 part, charcoal 30-40 part, complex micro organism fungicide 20-36 part, humic acid 15-20 part, bentonite 8-12 part, sodium potassium tartrate tetrahydrate 4-8 part, citric acid 4-8 part, copper sulfate 3-7 part, ferrous sulfate 2-6 part；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 10-18 part, Bacillus mucilaginosus agent 5-9 part, fixed nitrogen series bacillus agent 5-9 part.
As the further scheme of the present invention: the described composite microbiological fertilizer preventing and treating soil-borne disease, include according to the raw material of weight portion: discarded poultry feather 82-88 part, Testa oryzae 42-48 part, argol 42-48 part, weathered coal 42-48 part, charcoal 32-38 part, complex micro organism fungicide 24-32 part, humic acid 16-19 part, bentonite 9-11 part, sodium potassium tartrate tetrahydrate 5-7 part, citric acid 5-7 part, copper sulfate 4-6 part, ferrous sulfate 3-5 part；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 12-16 part, Bacillus mucilaginosus agent 6-8 part, fixed nitrogen series bacillus agent 6-8 part.
As the further scheme of the present invention: the described composite microbiological fertilizer preventing and treating soil-borne disease, include according to the raw material of weight portion: discarded 85 parts of poultry feather, 45 parts of Testa oryzae, argol 45 parts, weathered coal 45 parts, charcoal 35 parts, complex micro organism fungicide 28 parts, humic acid 18 parts, bentonite 10 parts, sodium potassium tartrate tetrahydrate 6 parts, citric acid 6 parts, 5 parts of copper sulfate, 4 parts of ferrous sulfate；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 14 parts, Bacillus mucilaginosus agent 7 parts, fixed nitrogen series bacillus agent 7 parts.
As the further scheme of the present invention: described bacillus megaterium agent includes bacillus megaterium and adsorbent, described Bacillus mucilaginosus agent includes Bacillus mucilaginosus and adsorbent, and described fixed nitrogen series bacillus agent includes fixed nitrogen series bacillus and adsorbent.
As the further scheme of the present invention: bacterium number respectively 1.8-2.2 × 10 of bacillus megaterium, Bacillus mucilaginosus and fixed nitrogen series bacillus in described complex micro organism fungicide7Individual/g, 1-1.4 × 107Individual/g, 7-9 × 106Individual/g.
As the further scheme of the present invention: described adsorbent is mixed by the Organic substance powder of 70% percentage by weight and the inorganic matter powder of 30% percentage by weight.
As the present invention further scheme: described Organic substance powder is at least one in Testa Tritici, Testa oryzae, soybean stem meal, bean cake, maize cob meal, sorghum husk powder, powder of straw, furfural；Described inorganic powder is at least one in zeolite, kieselguhr, Vermiculitum, attapulgite, precipitated calcium carbonate, ground phosphate rock or potassium breeze.
The preparation method of the described composite microbiological fertilizer preventing and treating soil-borne disease, specifically comprises the following steps that
(1) each raw material is weighed according to weight portion；
(2) respectively the adsorbent of bacillus megaterium bacterium solution, Bacillus mucilaginosus bacterium solution and fixed nitrogen series bacillus bacterium solution Yu composite microbial bacteria is sufficiently mixed stirring, adsorb into graininess bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent, bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent are uniformly mixed so as to obtain complex micro organism fungicide；
(3) it is divided into equiponderant two parts after discarded poultry feather being cleaned drying pulverizing, the a 3mol/L sulfuric acid solution adding 8-12 times of weight, it is placed in microwave oven microwave hydrolysis 3-4h, another part adds in the 6mol/L potassium hydroxide solution of 8-12 times of weight, is placed in microwave oven microwave hydrolysis 4-5h；
(4) two kinds of microwave hydrolysis liquid of step (3) gained are mixed and stirred for uniformly, it is filtrated to get hydrolysis clear liquid and organic detritus, citric acid, copper sulfate is added again in hydrolysis clear liquid, PH is adjusted to 6.0,60-80min is reacted at being heated to 55-65 DEG C, add ferrous sulfate and be warming up to 60-70 DEG C of reaction 50-60min, obtaining aminoacids complex sequestration liquid, standby；
(5) being mixed with argol, weathered coal, charcoal, Testa oryzae by step (4) gained organic detritus, put in fermentation vat, adding water and making fermentation heap water content control is 45-55%, compost fermentation 30-40d, takes out after fully becoming thoroughly decomposed；
(6) pass into the water of 10-12 times of weight and add complex micro organism fungicide in step (5) gained compost, stir and be placed in hermetic container and place 6-10d, then filter with double gauze, obtain lonely compost extracting solution；
(7) by described aminoacids complex sequestration liquid, be sick of compost extracting solution, humic acid, bentonite and sodium potassium tartrate tetrahydrate mixing and stirring, gained liquid fertilizer is placed in magnetizer under 350-450mT field intensity and magnetizes 8-12min, obtain composite microbiological fertilizer of the present invention.
Compared with prior art, the invention has the beneficial effects as follows:
Containing substantial amounts of all types of microorganisms in the present invention, with the addition of again complex micro organism fungicide with abundant probiotics content, composite microbiological fertilizer is made to become a microbial resources storehouse, structure of soil microbial community can be adjusted after drip irrigation sprays, improve diversity of soil microorganism, improving soil microenvironment, reducing the scope of phytopathogen, thus reaching to prevent and treat the purpose of soil-borne disease.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the technical scheme of this patent is described in more detail.
Embodiment 1
A kind of composite microbiological fertilizer preventing and treating soil-borne disease, includes according to the raw material of weight portion: discarded 80 parts of poultry feather, 40 parts of Testa oryzae, argol 40 parts, weathered coal 40 parts, charcoal 30 parts, complex micro organism fungicide 20 parts, humic acid 15 parts, bentonite 8 parts, sodium potassium tartrate tetrahydrate 4 parts, citric acid 4 parts, 3 parts of copper sulfate, 2 parts of ferrous sulfate；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 10 parts, Bacillus mucilaginosus agent 5 parts, fixed nitrogen series bacillus agent 5 parts；The bacterium number of bacillus megaterium, Bacillus mucilaginosus and fixed nitrogen series bacillus respectively 1.8 × 10 in described complex micro organism fungicide7Individual/g, 1 × 107Individual/g, 7 × 106Individual/g.
The preparation method of the described composite microbiological fertilizer preventing and treating soil-borne disease, specifically comprises the following steps that
(1) each raw material is weighed according to weight portion；
(2) respectively the adsorbent of bacillus megaterium bacterium solution, Bacillus mucilaginosus bacterium solution and fixed nitrogen series bacillus bacterium solution Yu composite microbial bacteria is sufficiently mixed stirring, adsorb into graininess bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent, bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent are uniformly mixed so as to obtain complex micro organism fungicide；
(3) it is divided into equiponderant two parts after discarded poultry feather being cleaned drying pulverizing, the a 3mol/L sulfuric acid solution adding 8 times of weight, being placed in microwave oven microwave hydrolysis 3h, another part adds in the 6mol/L potassium hydroxide solution of 8 times of weight, is placed in microwave oven microwave hydrolysis 4h；
(4) two kinds of microwave hydrolysis liquid of step (3) gained are mixed and stirred for uniformly, it is filtrated to get hydrolysis clear liquid and organic detritus, citric acid, copper sulfate is added again in hydrolysis clear liquid, PH is adjusted to 6.0, it is heated at 55 DEG C and reacts 60min, add ferrous sulfate and be warming up to 60 DEG C of reaction 50min, obtaining aminoacids complex sequestration liquid, standby；
(5) being mixed with argol, weathered coal, charcoal, Testa oryzae by step (4) gained organic detritus, put in fermentation vat, adding water and making fermentation heap water content control is 45%, compost fermentation 30d, takes out after fully becoming thoroughly decomposed；
(6) pass into the water of 10 times of weight and add complex micro organism fungicide in step (5) gained compost, stir and be placed in hermetic container and place 6d, then filter with double gauze, obtain lonely compost extracting solution；
(7) by described aminoacids complex sequestration liquid, be sick of compost extracting solution, humic acid, bentonite and sodium potassium tartrate tetrahydrate mixing and stirring, gained liquid fertilizer is placed in magnetizer under 350mT field intensity and magnetizes 8min, obtain composite microbiological fertilizer of the present invention.
Embodiment 2
A kind of composite microbiological fertilizer preventing and treating soil-borne disease, includes according to the raw material of weight portion: discarded 82 parts of poultry feather, 42 parts of Testa oryzae, argol 42 parts, weathered coal 42 parts, charcoal 32 parts, complex micro organism fungicide 24 parts, humic acid 16 parts, bentonite 9 parts, sodium potassium tartrate tetrahydrate 5 parts, citric acid 5 parts, 4 parts of copper sulfate, 3 parts of ferrous sulfate；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 12 parts, Bacillus mucilaginosus agent 6 parts, fixed nitrogen series bacillus agent 6 parts；The bacterium number of bacillus megaterium, Bacillus mucilaginosus and fixed nitrogen series bacillus respectively 1.9 × 10 in described complex micro organism fungicide7Individual/g, 1.1 × 107Individual/g, 7.5 × 106Individual/g.
The preparation method of the described composite microbiological fertilizer preventing and treating soil-borne disease, specifically comprises the following steps that
(1) each raw material is weighed according to weight portion；
(2) respectively the adsorbent of bacillus megaterium bacterium solution, Bacillus mucilaginosus bacterium solution and fixed nitrogen series bacillus bacterium solution Yu composite microbial bacteria is sufficiently mixed stirring, adsorb into graininess bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent, bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent are uniformly mixed so as to obtain complex micro organism fungicide；
(3) it is divided into equiponderant two parts after discarded poultry feather being cleaned drying pulverizing, the a 3mol/L sulfuric acid solution adding 9 times of weight, being placed in microwave oven microwave hydrolysis 3.2h, another part adds in the 6mol/L potassium hydroxide solution of 9 times of weight, is placed in microwave oven microwave hydrolysis 4.2h；
(4) two kinds of microwave hydrolysis liquid of step (3) gained are mixed and stirred for uniformly, it is filtrated to get hydrolysis clear liquid and organic detritus, citric acid, copper sulfate is added again in hydrolysis clear liquid, PH is adjusted to 6.0, it is heated at 58 DEG C and reacts 65min, add ferrous sulfate and be warming up to 62 DEG C of reaction 52min, obtaining aminoacids complex sequestration liquid, standby；
(5) being mixed with argol, weathered coal, charcoal, Testa oryzae by step (4) gained organic detritus, put in fermentation vat, adding water and making fermentation heap water content control is 48%, compost fermentation 32d, takes out after fully becoming thoroughly decomposed；
(6) pass into the water of 10.5 times of weight and add complex micro organism fungicide in step (5) gained compost, stir and be placed in hermetic container and place 7d, then filter with double gauze, obtain lonely compost extracting solution；
(7) by described aminoacids complex sequestration liquid, be sick of compost extracting solution, humic acid, bentonite and sodium potassium tartrate tetrahydrate mixing and stirring, gained liquid fertilizer is placed in magnetizer under 380mT field intensity and magnetizes 9min, obtain composite microbiological fertilizer of the present invention.
Embodiment 3
A kind of composite microbiological fertilizer preventing and treating soil-borne disease, includes according to the raw material of weight portion: discarded 85 parts of poultry feather, 45 parts of Testa oryzae, argol 45 parts, weathered coal 45 parts, charcoal 35 parts, complex micro organism fungicide 28 parts, humic acid 18 parts, bentonite 10 parts, sodium potassium tartrate tetrahydrate 6 parts, citric acid 6 parts, 5 parts of copper sulfate, 4 parts of ferrous sulfate；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 14 parts, Bacillus mucilaginosus agent 7 parts, fixed nitrogen series bacillus agent 7 parts；The bacterium number of bacillus megaterium, Bacillus mucilaginosus and fixed nitrogen series bacillus respectively 2 × 10 in described complex micro organism fungicide7Individual/g, 1.2 × 107Individual/g, 8 × 106Individual/g.
The preparation method of the described composite microbiological fertilizer preventing and treating soil-borne disease, specifically comprises the following steps that
(1) each raw material is weighed according to weight portion；
(2) respectively the adsorbent of bacillus megaterium bacterium solution, Bacillus mucilaginosus bacterium solution and fixed nitrogen series bacillus bacterium solution Yu composite microbial bacteria is sufficiently mixed stirring, adsorb into graininess bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent, bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent are uniformly mixed so as to obtain complex micro organism fungicide；
(3) it is divided into equiponderant two parts after discarded poultry feather being cleaned drying pulverizing, the a 3mol/L sulfuric acid solution adding 10 times of weight, being placed in microwave oven microwave hydrolysis 3.5h, another part adds in the 6mol/L potassium hydroxide solution of 10 times of weight, is placed in microwave oven microwave hydrolysis 4.5h；
(4) two kinds of microwave hydrolysis liquid of step (3) gained are mixed and stirred for uniformly, it is filtrated to get hydrolysis clear liquid and organic detritus, citric acid, copper sulfate is added again in hydrolysis clear liquid, PH is adjusted to 6.0, it is heated at 60 DEG C and reacts 70min, add ferrous sulfate and be warming up to 65 DEG C of reaction 55min, obtaining aminoacids complex sequestration liquid, standby；
(5) being mixed with argol, weathered coal, charcoal, Testa oryzae by step (4) gained organic detritus, put in fermentation vat, adding water and making fermentation heap water content control is 50%, compost fermentation 35d, takes out after fully becoming thoroughly decomposed；
(6) pass into the water of 11 times of weight and add complex micro organism fungicide in step (5) gained compost, stir and be placed in hermetic container and place 8d, then filter with double gauze, obtain lonely compost extracting solution；
(7) by described aminoacids complex sequestration liquid, be sick of compost extracting solution, humic acid, bentonite and sodium potassium tartrate tetrahydrate mixing and stirring, gained liquid fertilizer is placed in magnetizer under 400mT field intensity and magnetizes 10min, obtain composite microbiological fertilizer of the present invention.
Embodiment 4
A kind of composite microbiological fertilizer preventing and treating soil-borne disease, includes according to the raw material of weight portion: discarded 88 parts of poultry feather, 48 parts of Testa oryzae, argol 48 parts, weathered coal 48 parts, charcoal 38 parts, complex micro organism fungicide 32 parts, humic acid 19 parts, bentonite 11 parts, sodium potassium tartrate tetrahydrate 7 parts, citric acid 7 parts, 6 parts of copper sulfate, 5 parts of ferrous sulfate: described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 16 parts, Bacillus mucilaginosus agent 8 parts, fixed nitrogen series bacillus agent 8 parts；The bacterium number of bacillus megaterium, Bacillus mucilaginosus and fixed nitrogen series bacillus respectively 2.1 × 10 in described complex micro organism fungicide7Individual/g, 1.3 × 107Individual/g, 8.5 × 106Individual/g.
The preparation method of the described composite microbiological fertilizer preventing and treating soil-borne disease, specifically comprises the following steps that
(1) each raw material is weighed according to weight portion；
(2) respectively the adsorbent of bacillus megaterium bacterium solution, Bacillus mucilaginosus bacterium solution and fixed nitrogen series bacillus bacterium solution Yu composite microbial bacteria is sufficiently mixed stirring, adsorb into graininess bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent, bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent are uniformly mixed so as to obtain complex micro organism fungicide；
(3) it is divided into equiponderant two parts after discarded poultry feather being cleaned drying pulverizing, the a 3mol/L sulfuric acid solution adding 11 times of weight, being placed in microwave oven microwave hydrolysis 3.8h, another part adds in the 6mol/L potassium hydroxide solution of 11 times of weight, is placed in microwave oven microwave hydrolysis 4.8h；
(4) two kinds of microwave hydrolysis liquid of step (3) gained are mixed and stirred for uniformly, it is filtrated to get hydrolysis clear liquid and organic detritus, citric acid, copper sulfate is added again in hydrolysis clear liquid, PH is adjusted to 6.0, it is heated at 62 DEG C and reacts 75min, add ferrous sulfate and be warming up to 68 DEG C of reaction 58min, obtaining aminoacids complex sequestration liquid, standby；
(5) being mixed with argol, weathered coal, charcoal, Testa oryzae by step (4) gained organic detritus, put in fermentation vat, adding water and making fermentation heap water content control is 52%, compost fermentation 38d, takes out after fully becoming thoroughly decomposed；
(6) pass into the water of 11.5 times of weight and add complex micro organism fungicide in step (5) gained compost, stir and be placed in hermetic container and place 9d, then filter with double gauze, obtain lonely compost extracting solution；
(7) by described aminoacids complex sequestration liquid, be sick of compost extracting solution, humic acid, bentonite and sodium potassium tartrate tetrahydrate mixing and stirring, gained liquid fertilizer is placed in magnetizer under 420mT field intensity and magnetizes 11min, obtain composite microbiological fertilizer of the present invention.
Embodiment 5
A kind of composite microbiological fertilizer preventing and treating soil-borne disease, includes according to the raw material of weight portion: discarded 90 parts of poultry feather, 50 parts of Testa oryzae, argol 50 parts, weathered coal 50 parts, charcoal 40 parts, complex micro organism fungicide 36 parts, humic acid 20 parts, bentonite 12 parts, sodium potassium tartrate tetrahydrate 8 parts, citric acid 8 parts, 7 parts of copper sulfate, 6 parts of ferrous sulfate；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 18 parts, Bacillus mucilaginosus agent 9 parts, fixed nitrogen series bacillus agent 9 parts；The bacterium number of bacillus megaterium, Bacillus mucilaginosus and fixed nitrogen series bacillus respectively 2.2 × 10 in described complex micro organism fungicide7Individual/g, 1.4 × 107Individual/g, 9 × 106Individual/g.
The preparation method of the described composite microbiological fertilizer preventing and treating soil-borne disease, specifically comprises the following steps that
(1) each raw material is weighed according to weight portion；
(2) respectively the adsorbent of bacillus megaterium bacterium solution, Bacillus mucilaginosus bacterium solution and fixed nitrogen series bacillus bacterium solution Yu composite microbial bacteria is sufficiently mixed stirring, adsorb into graininess bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent, bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent are uniformly mixed so as to obtain complex micro organism fungicide；
(3) it is divided into equiponderant two parts after discarded poultry feather being cleaned drying pulverizing, the a 3mol/L sulfuric acid solution adding 12 times of weight, being placed in microwave oven microwave hydrolysis 4h, another part adds in the 6mol/L potassium hydroxide solution of 12 times of weight, is placed in microwave oven microwave hydrolysis 5h；
(4) two kinds of microwave hydrolysis liquid of step (3) gained are mixed and stirred for uniformly, it is filtrated to get hydrolysis clear liquid and organic detritus, citric acid, copper sulfate is added again in hydrolysis clear liquid, PH is adjusted to 6.0, it is heated at 65 DEG C and reacts 80min, add ferrous sulfate and be warming up to 70 DEG C of reaction 60min, obtaining aminoacids complex sequestration liquid, standby；
(5) being mixed with argol, weathered coal, charcoal, Testa oryzae by step (4) gained organic detritus, put in fermentation vat, adding water and making fermentation heap water content control is 55%, compost fermentation 40d, takes out after fully becoming thoroughly decomposed；
(6) pass into the water of 12 times of weight and add complex micro organism fungicide in step (5) gained compost, stir and be placed in hermetic container and place 10d, then filter with double gauze, obtain lonely compost extracting solution；
(7) by described aminoacids complex sequestration liquid, be sick of compost extracting solution, humic acid, bentonite and sodium potassium tartrate tetrahydrate mixing and stirring, gained liquid fertilizer is placed in magnetizer under 450mT field intensity and magnetizes 12min, obtain composite microbiological fertilizer of the present invention.
Described bacillus megaterium agent includes bacillus megaterium and adsorbent, and described Bacillus mucilaginosus agent includes Bacillus mucilaginosus and adsorbent, and described fixed nitrogen series bacillus agent includes fixed nitrogen series bacillus and adsorbent；As the further scheme of the present invention: described adsorbent is mixed by the Organic substance powder of 70% percentage by weight and the inorganic matter powder of 30% percentage by weight；Described Organic substance powder is at least one in Testa Tritici, Testa oryzae, soybean stem meal, bean cake, maize cob meal, sorghum husk powder, powder of straw, furfural；Described inorganic powder is at least one in zeolite, kieselguhr, Vermiculitum, attapulgite, precipitated calcium carbonate, ground phosphate rock or potassium breeze.
Containing substantial amounts of all types of microorganisms in the present invention, with the addition of again complex micro organism fungicide with abundant probiotics content, composite microbiological fertilizer is made to become a microbial resources storehouse, structure of soil microbial community can be adjusted after drip irrigation sprays, improve diversity of soil microorganism, improving soil microenvironment, reducing the scope of phytopathogen, thus reaching to prevent and treat the purpose of soil-borne disease.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment, in the ken that one skilled in the relevant art possesses, it is also possible under the premise without departing from this patent objective, make various change.
Claims (8)
1. the composite microbiological fertilizer that can prevent and treat soil-borne disease, it is characterized in that, include according to the raw material of weight portion: discarded poultry feather 80-90 part, Testa oryzae 40-50 part, argol 40-50 part, weathered coal 40-50 part, charcoal 30-40 part, complex micro organism fungicide 20-36 part, humic acid 15-20 part, bentonite 8-12 part, sodium potassium tartrate tetrahydrate 4-8 part, citric acid 4-8 part, copper sulfate 3-7 part, ferrous sulfate 2-6 part；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 10-18 part, Bacillus mucilaginosus agent 5-9 part, fixed nitrogen series bacillus agent 5-9 part.
2. the composite microbiological fertilizer preventing and treating soil-borne disease according to claim 1, it is characterized in that, include according to the raw material of weight portion: discarded poultry feather 82-88 part, Testa oryzae 42-48 part, argol 42-48 part, weathered coal 42-48 part, charcoal 32-38 part, complex micro organism fungicide 24-32 part, humic acid 16-19 part, bentonite 9-11 part, sodium potassium tartrate tetrahydrate 5-7 part, citric acid 5-7 part, copper sulfate 4-6 part, ferrous sulfate 3-5 part；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 12-16 part, Bacillus mucilaginosus agent 6-8 part, fixed nitrogen series bacillus agent 6-8 part.
3. the composite microbiological fertilizer preventing and treating soil-borne disease according to claim 1, it is characterized in that, include according to the raw material of weight portion: discarded 85 parts of poultry feather, 45 parts of Testa oryzae, argol 45 parts, weathered coal 45 parts, charcoal 35 parts, complex micro organism fungicide 28 parts, humic acid 18 parts, bentonite 10 parts, sodium potassium tartrate tetrahydrate 6 parts, citric acid 6 parts, 5 parts of copper sulfate, 4 parts of ferrous sulfate；Described complex micro organism fungicide includes according to the raw material of weight portion: bacillus megaterium agent 14 parts, Bacillus mucilaginosus agent 7 parts, fixed nitrogen series bacillus agent 7 parts.
4. the composite microbiological fertilizer according to arbitrary described the prevented and treated soil-borne disease of claim 1-3, it is characterized in that, described bacillus megaterium agent includes bacillus megaterium and adsorbent, described Bacillus mucilaginosus agent includes Bacillus mucilaginosus and adsorbent, and described fixed nitrogen series bacillus agent includes fixed nitrogen series bacillus and adsorbent.
5. the composite microbiological fertilizer according to arbitrary described the prevented and treated soil-borne disease of claim 1-3, it is characterised in that bacterium number respectively 1.8-2.2 × 10 of bacillus megaterium, Bacillus mucilaginosus and fixed nitrogen series bacillus in described complex micro organism fungicide7Individual/g, 1-1.4 × 107Individual/g, 7-9 × 106Individual/g.
6. the composite microbiological fertilizer preventing and treating soil-borne disease according to claim 4, it is characterised in that described adsorbent is mixed by the Organic substance powder of 70% percentage by weight and the inorganic matter powder of 30% percentage by weight.
7. the composite microbiological fertilizer preventing and treating soil-borne disease according to claim 6, it is characterised in that described Organic substance powder is at least one in Testa Tritici, Testa oryzae, soybean stem meal, bean cake, maize cob meal, sorghum husk powder, powder of straw, furfural；Described inorganic powder is at least one in zeolite, kieselguhr, Vermiculitum, attapulgite, precipitated calcium carbonate, ground phosphate rock or potassium breeze.
8. one kind as arbitrary in claim 1-3 as described in the preparation method of composite microbiological fertilizer of prevented and treated soil-borne disease, it is characterised in that specifically comprise the following steps that
(1) each raw material is weighed according to weight portion；
(2) respectively the adsorbent of bacillus megaterium bacterium solution, Bacillus mucilaginosus bacterium solution and fixed nitrogen series bacillus bacterium solution Yu composite microbial bacteria is sufficiently mixed stirring, adsorb into graininess bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent, bacillus megaterium microbial inoculum, Bacillus mucilaginosus agent and fixed nitrogen series bacillus agent are uniformly mixed so as to obtain complex micro organism fungicide；
(3) it is divided into equiponderant two parts after discarded poultry feather being cleaned drying pulverizing, the a 3mol/L sulfuric acid solution adding 8-12 times of weight, it is placed in microwave oven microwave hydrolysis 3-4h, another part adds in the 6mol/L potassium hydroxide solution of 8-12 times of weight, is placed in microwave oven microwave hydrolysis 4-5h；
(4) two kinds of microwave hydrolysis liquid of step (3) gained are mixed and stirred for uniformly, it is filtrated to get hydrolysis clear liquid and organic detritus, citric acid, copper sulfate is added again in hydrolysis clear liquid, PH is adjusted to 6.0,60-80min is reacted at being heated to 55-65 DEG C, add ferrous sulfate and be warming up to 60-70 DEG C of reaction 50-60min, obtaining aminoacids complex sequestration liquid, standby；
(5) being mixed with argol, weathered coal, charcoal, Testa oryzae by step (4) gained organic detritus, put in fermentation vat, adding water and making fermentation heap water content control is 45-55%, compost fermentation 30-40d, takes out after fully becoming thoroughly decomposed；
(6) pass into the water of 10-12 times of weight and add complex micro organism fungicide in step (5) gained compost, stir and be placed in hermetic container and place 6-10d, then filter with double gauze, obtain lonely compost extracting solution；
(7) by described aminoacids complex sequestration liquid, be sick of compost extracting solution, humic acid, bentonite and sodium potassium tartrate tetrahydrate mixing and stirring, gained liquid fertilizer is placed in magnetizer under 350-450mT field intensity and magnetizes 8-12min, obtain composite microbiological fertilizer of the present invention.
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