CN107466751A - A kind of implantation methods for improving rice quality and preparation method thereof - Google Patents

A kind of implantation methods for improving rice quality and preparation method thereof Download PDF

Info

Publication number
CN107466751A
CN107466751A CN201710641831.0A CN201710641831A CN107466751A CN 107466751 A CN107466751 A CN 107466751A CN 201710641831 A CN201710641831 A CN 201710641831A CN 107466751 A CN107466751 A CN 107466751A
Authority
CN
China
Prior art keywords
implantation methods
bacterium
saccharomycete
individual
nitric acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710641831.0A
Other languages
Chinese (zh)
Inventor
李达耀
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pingnan County Zhengda Agriculture Development Co Ltd
Original Assignee
Pingnan County Zhengda Agriculture Development Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pingnan County Zhengda Agriculture Development Co Ltd filed Critical Pingnan County Zhengda Agriculture Development Co Ltd
Priority to CN201710641831.0A priority Critical patent/CN107466751A/en
Publication of CN107466751A publication Critical patent/CN107466751A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A01G22/22—Rice
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to agricultural technology field, more particularly to a kind of implantation methods for improving rice quality and preparation method thereof, fertilizer includes bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite, Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide, saccharomycete, nitric acid needle-like bacterium, thread sulfur bacteria, bacillus megaterium and is made according to certain percentage；Preparation method is material mixed fermentation first to cultivate micro- raw bacterium of effective dose.It is an advantage of the invention that preparation method is simple, each element in fertilizer is sufficient, is adapted to element needed for the seedling growth of seedling phase, premunition is improved since seedling phase rice shoot, so as to reach the purpose of volume increase.

Description

A kind of implantation methods for improving rice quality and preparation method thereof
【Technical field】
The present invention relates to agricultural technology field, and in particular to a kind of implantation methods for improving rice quality and its preparation side Method.
【Background technology】
China is a large agricultural country, and rice cultivation has had the history of more than one thousand years, and its key link of rice cultivation is Seedling, the quality that big rice shoot is obtained after seedling directly affects yield cultivated behind later stage field planting crop field etc., and therefore, exploitation is a kind of Brand-new special fertilizer for raising rice seedlings material is the task of top priority.
【The content of the invention】
The goal of the invention of the present invention is：A kind of implantation methods for improving rice quality and preparation method thereof, method are provided It is simple and easy, there is great generalization, seedling fertilizer can be disease-resistant, improves the survival rate and resistance against diseases of rice shoot, so as to fixed Pest and disease damage Probability is reduced after planting crop field, improves the quality of rice.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of implantation methods for improving rice quality, the implantation methods of the raising rice quality are according to percent by weight bag Include 10%-20% bagasse, 8%-15% furfural dregs, 10%-20% soybean slags, 5%-10% phosphorus ores slag, 5%-10% biologies Charcoal, 5%-10% nitrate fertilizers, 2%-8% humic acids, 3%-5% chondrodites, 2%-5% Cynanchum Komarrivii AI Iijiniskis, 1%-5% saltbush, 1%-5% seas silkweed, 1%-5% kuh-sengs, 1%-5% Sophora alopecuroides, 2%-3% saccharomycete, 1%-2% nitric acid needle-likes bacterium, 1%- 2% thread sulfur bacteria, 1%-2% bacillus megateriums.
Preferably, the implantation methods of the raising rice quality according to percent by weight include 12%-18% bagasse, 10%-14% furfural dregs, 12%-18% soybean slags, 6%-9% phosphorus ores slag, 6%-9% charcoals, 6%-9% nitrate fertilizers, 3%-7% humic acids, 3.5%-4.5% chondrodites, 2.5%-4.5% Cynanchum Komarrivii AI Iijiniskis, 1.5%-4.5% saltbush, 2%-4.5% Extra large silkweed, 1.5%-4.5% kuh-sengs, 1.5%-4.5% Sophora alopecuroides, 2.5%-2.9% saccharomycete, 1.2%-1.9% nitric acid pins The thread sulfur bacteria of shape bacterium, 1.1%-1.9%, 1.3%-2.6% bacillus megateriums.
It is more preferred, the implantation methods of the raising rice quality according to percent by weight include 17% bagasse, 12% furfural dregs, 16% soybean slag, 7% phosphorus ore slag, 8% charcoal, 7% nitrate fertilizer, 5% humic acid, 4% chondrodite, 2.8% Cynanchum Komarrivii AI Iijiniski, 3.5% saltbush, 3.6% extra large silkweed, 2.6% kuh-seng, 3.6% Sophora alopecuroide, 2.6% saccharomycete, 1.7% nitric acid Needle-like bacterium, 1.8% thread sulfur bacteria, 1.8% bacillus megaterium.
Further illustrate, the effective bacteria containing amount of saccharomycete is 2.15 × 108-3.21×108Individual/mL, nitric acid needle-like bacterium have It is 1.15 × 10 to imitate bacteria containing amount8-2.15×108Individual/mL, the effective bacteria containing amount of thread sulfur bacteria are 1.11 × 108-3.45×108 The effective bacteria containing amount of individual/mL, bacillus megaterium is 3.22 × 108-5.16×108Individual/mL.
A kind of preparation method of implantation methods for improving rice quality as described above, including step in detail below：
S1, by peptone 18g, brewer's wort 15g, beef extract 8g, glucose 15g, the agar (W/V) 1.2% of 7 degree of Baumes, 1000ml is added water to, saccharomycete is inoculated in liquid training by pH6-6.5 as fluid nutrient medium, then in terms of v/v 5% inoculum concentration Can obtain effective bacteria containing amount after fermentation 20-24 hours under conditions of 25 DEG C of temperature, rotating speed 300rpm in foster base is
2.15×108-3.21×108Individual/mL saccharomycete；
S2, by NaHCO3 0.34g、NH4Cl 0.13g、MgSO4·7H2O 0.25g、K2HPO4 1.35g、Na(CH3COO) 1.85g、KNO3 1.25g、Na2S·9H2O 3g add water to 10ml, and pH8-8.5 is as fluid nutrient medium, and inoculum concentration is according to v/v 25-30h is cultivated under conditions of 28 DEG C, you can obtain effective bacteria containing amount as 1.15 × 10 for 10% addition nitric acid needle-like bacterium8- 2.15×108Individual/mL nitric acid needle-like bacterium；
S3, by EDTA 12g, NaHCO3 15g、FeCl2·4H2O 10g、MnCl2·2H2O 20g、K2HPO4 1.35g、 (NH4)6Mo7O24·4H2O 16g、KNO3 15g、Na2S·9H2O 12g, agar 10g add water to 1000ml, pH8-8.5 conducts Fluid nutrient medium, inoculum concentration are that 10% plus thread sulfur bacteria cultivate 25-30h under conditions of 28 DEG C according to using v/v, you can are obtained Effective bacteria containing amount is 1.11 × 108-3.45×108Individual/mL thread sulfur bacteria；
S4, brewer's wort 1%, sodium chloride 3%, dipotassium hydrogen phosphate 0.3%, glucose 2%, iron chloride 0.5%, ammonium sulfate 0.02%th, magnesium sulfate 0.03%, dusty yeast 0.08%, agar 0.1%, calcium carbonate 0.1%, excess water polishing to 100%, configuration Into 1000ml fluid nutrient medium, inoculum concentration is that 8% plus bacillus megaterium cultivate 25- under conditions of 30 DEG C according to using v/v 30h, you can obtain effective bacteria containing amount as 3.22 × 108-5.16×108Individual/mL bacillus megateriums；
S5, according to percent by weight, the saccharomycete that above-mentioned steps culture is obtained, nitric acid needle-like bacterium, thread sulfur bacteria, Bacillus megaterium builds heap with bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite Fermentation can obtain base manure in 10 days；
S6, according to percent by weight, by the base manure of above-mentioned steps and Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide The implantation methods of rice quality can be improved after mixed fermentation 15-25 hours.
Further illustrate, the fermentation condition of the S5 steps is that temperature is 30-32 DEG C.
Further illustrate, the fermentation condition of the S6 steps is that temperature is 27-30 DEG C.
Further illustrate, Cynanchum Komarrivii AI Iijiniski uses Cynanchum Komarrivii AI Iijiniski refining liquid in the present invention, according to quality after Cynanchum Komarrivii AI Iijiniski is crushed Than for 1:10-20 ratios add water carry out microwave abstracting extract to obtain crude extract, be subsequently added into ethanol solution standard atmospheric pressure, Distilled at 80-90 DEG C, removal slips out thing, and it is Cynanchum Komarrivii AI Iijiniski refining liquid to obtain residue.
Further illustrate, saltbush uses saltbush extract solution in the present invention, and saltbush shines dry grinding, adds 3-5 times of volume Mass concentration be 75% ethanol soak 5 minutes, then extract 35 minutes at 65 DEG C, filtering, residue soaks at 65 DEG C again Carry 35 minutes, the extract solution merged twice can obtain saltbush extract solution.
Further illustrate, extra large silkweed uses extra large silkweed extract in the present invention, after extra large silkweed complete stool is shone into dry grinding The degreasing of progress soxhlet extraction, water extract-alcohol precipitation, centrifugation, water-bath are volatilized, are freeze-dried successively, produce extra large silkweed extract.
Further illustrate, kuh-seng uses Radix Sophorae Flavescentis extractive liquid in the present invention, is 3-5 times of kuh-seng by kuh-seng addition volume ratio Mass concentration be 75% ethanol in be ground 3-5 minutes, then the mixed liquor after grinding is extracted, circulate Repeat 3-5 times, remember to obtain Radix Sophorae Flavescentis extractive liquid.
Further illustrate, Sophora alopecuroide uses Sophora alopecuroide extract solution in the present invention, is added after Sophora alopecuroide is cleaned appropriate Water is beaten, then add lauryl sodium sulfate soak 20 hours, then pressure be 0.8-0.9MPa, temperature 30- Cohobation 20-30 minutes are carried out under the conditions of 40 DEG C, it is Sophora alopecuroide extract solution then to carry out press filtration and obtain filtrate.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Bagasse, furfural dregs in the application are that slant acidity is neutralized, it is necessary to add some alkaline matters, otherwise mistake The growth of nitric acid needle-like bacterium, thread sulfur bacteria, Bacillus megatherium can be suppressed under the environment of acid, so as to cut down full fermentation significantly Speed, bagasse, one that furfural dregs are presented can be neutralized to a certain extent after researcher has found by with addition of soybean slag It is a little acid, cut down pH numerical value so that a suitable prolific environment of beneficial microbe is presented in overall raw material, so as to contract The time of short overall raw material full fermentation, but in the fermentation process of entirety, not exclusively the pH value of environment can be influenceed beneficial to micro- Whether the growth of biology is comprehensive so as to influence fermentation, and beneficial microorganism breeding also needs to substantial amounts of energy element, and in bagasse Directly the energy can be improved in fermentation with the molecular forms of glucose initial stage, beneficial microbe can directly be inhaled containing sugar material Receive using provide the energy element, then beneficial microbe amount reproduction and decompose Organic Ingredients release raw material in carbon source, nitrogen source, phosphorus Source, there is trace element, a carbon source part for release continues to absorb amount reproduction by beneficial microbe, ground by researcher Discovery is studied carefully during seedling, if there is P elements, element silicon, magnesium elements shortage, the probability that disease occurs in rice shoot is big, planting percent Low, often present seedling fertilizer occurs as soon as these elements and lacked or the bad phenomenon for causing planting percent not high of ratio, addition 5%-10% phosphorus ores slag, 3%-5% chondrodites reach after full fermentation is decomposed by detecting total phosphorus content in seedling fertilizer 13%, containing rapid available phosphorus 85-95ppm, effective silicon reaches 15-17%, and magnesium element content reaches 55-68.5ppm；Bagasse contains 45-50% fiber, crude protein >=8.5%, sugared content reach 13%, by the way that there is splendid organic substance after decomposition of fermenting, Furfural dregs are rich in vitamin, lignin and a small amount of sulfuric acid, with addition of thread sulfur bacteria can use and decompose a small amount of sulfuric acid, change give up into Treasured, the fiber containing 12-22% in soybean slag, protein >=32%, amino acid >=30%, content is largely divided greatly in humic acid The basic structure of son, such as aromatic ring and alicyclic ring, the functional groups such as carboxyl, hydroxyl, carbonyl, quinonyl, methoxyl group is connected with ring, but greatly Molecule is difficult to directly be absorbed use by plant, and fraction can absorb, and greatly reduce utilization rate, and the application makes by research The molecular substance of macoradical can more thoroughly be decomposed with addition of bacillus megaterium with addition of discovery after fermentation with multiple-microorganism For small molecule, and after ensureing the amount of breeding with addition of the external environment for adjusting fermentation after a certain amount again, can decompose largely fine Dimension element, the material such as protein is absorbed after the STRUCTURE DECOMPOSITION that uses is small molecule group from the difficult of macromolecule, preferably by rice shoot Use is absorbed, and bacillus megaterium can decompose the content of the P elements increase available phosphorus in raw material；During the fermentation A series of biochemical reactions of generation wherein just include nitrous acid caused by nitrification, and its toxicity is very strong, if in the environment Accumulate, be all poisonous to plant and other microorganisms, thus with addition of nitric acid needle-like bacterium so as to by it is nitrite-oxidizing into Nitric acid, harmless use can be absorbed by plant, and during whole full fermentation, it is found by the applicant that can decompose therewith useless The heavy metal element contained in raw material is abandoned, this can bring harm to the growth of rice shoot, and the absorption of heavy metal can also restrict it The uptake of his healthy trace elements with household, property is carried out to the fertilizer after full fermentation so as to add charcoal absorption heavy metal element Improvement, so as to improve the quality of rice so that rice all greatly improves in each healthy trace elements with household content and yield；Complete With the addition of in base manure after fermentation, 2%-3% Cynanchum Komarrivii AI Iijiniskis, 1%-5% saltbush, 1%-3% seas silkweed, 1%-3% kuh-sengs, 1%- 2% Sophora alopecuroide can control the growth of harmful microorganism flora, expand the scope of disease and insect resistance, greatly reduce the disease in later stage Rate, and the obvious approach that disease rate improves yield that reduces is confirmed from experiment.
【Embodiment】
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below Embodiment is closed, the present invention is expanded on further.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, It is not intended to limit the present invention.
Embodiment 1：
A kind of implantation methods for improving rice quality, the implantation methods of the raising rice quality are according to percent by weight bag Include 15% bagasse, 8% furfural dregs, 15% soybean slag, 10% phosphorus ore slag, 10% charcoal, 5% nitrate fertilizer, 2% rotten plant Acid, 5% chondrodite, 5% Cynanchum Komarrivii AI Iijiniski, 5% saltbush, 5% extra large silkweed, 5% kuh-seng, 5% Sophora alopecuroide, 2% saccharomycete, 1% nitre Sour needle-like bacterium, 1% thread sulfur bacteria, 1% bacillus megaterium.
The raw material of aforementioned proportion is used to prepare a kind of method for the implantation methods for improving rice quality, including in detail below Step：
S1, by peptone 18g, brewer's wort 15g, beef extract 8g, glucose 15g, the agar (W/V) 1.2% of 7 degree of Baumes, 1000ml is added water to, saccharomycete is inoculated in Liquid Culture by pH6.2 as fluid nutrient medium, then in terms of v/v 5% inoculum concentration Effective bacteria containing amount is can obtain after being fermented 22 hours under conditions of 25 DEG C of temperature, rotating speed 300rpm in base as 2.65 × 108Individual/ ML saccharomycete；
S2, by NaHCO3 0.34g、NH4Cl 0.13g、MgSO4·7H2O 0.25g、K2HPO4 1.35g、Na(CH3COO) 1.85g、KNO3 1.25g、Na2S·9H2O 3g add water to 10ml, and pH8-8.5 is as fluid nutrient medium, and inoculum concentration is according to v/v 25-30h is cultivated under conditions of 28 DEG C, you can obtain effective bacteria containing amount as 1.15 × 10 for 10% addition nitric acid needle-like bacterium8- 2.15×108Individual/mL nitric acid needle-like bacterium；
S3, by EDTA 12g, NaHCO3 15g、FeCl2·4H2O 10g、MnCl2·2H2O 20g、K2HPO4 1.35g、 (NH4)6Mo7O24·4H2O 16g、KNO3 15g、Na2S·9H2O 12g, agar 10g add water to 1000ml, pH8-8.5 conducts Fluid nutrient medium, inoculum concentration are that 10% plus thread sulfur bacteria cultivate 28h under conditions of 28 DEG C according to using v/v, you can are had It is 2.25 × 10 to imitate bacteria containing amount8Individual/mL thread sulfur bacteria；
S4, brewer's wort 1%, sodium chloride 3%, dipotassium hydrogen phosphate 0.3%, glucose 2%, iron chloride 0.5%, ammonium sulfate 0.02%th, magnesium sulfate 0.03%, dusty yeast 0.08%, agar 0.1%, calcium carbonate 0.1%, excess water polishing to 100%, configuration Into 1000ml fluid nutrient medium, inoculum concentration according to using v/v be 8% plus bacillus megaterium cultivate 28h under conditions of 30 DEG C, Effective bacteria containing amount be can obtain as 4.12 × 108Individual/mL bacillus megateriums；
S5, according to percent by weight, the saccharomycete that above-mentioned steps culture is obtained, nitric acid needle-like bacterium, thread sulfur bacteria, Bacillus megaterium builds heap with bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite Temperature is that 31 DEG C of fermentations can obtain base manure in 10 days；
S6, according to percent by weight, by the base manure of above-mentioned steps and Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide Mixing temperature can be improved the implantation methods of rice quality after being fermented 18 hours for 28 DEG C.
Further illustrate, Cynanchum Komarrivii AI Iijiniski uses Cynanchum Komarrivii AI Iijiniski refining liquid in the present invention, according to quality after Cynanchum Komarrivii AI Iijiniski is crushed Than for 1:15 ratios add water progress microwave abstracting and extract to obtain crude extract, are subsequently added into ethanol solution in standard atmospheric pressure, 85 Distilled at DEG C, removal slips out thing, and it is Cynanchum Komarrivii AI Iijiniski refining liquid to obtain residue.
Further illustrate, saltbush uses saltbush extract solution in the present invention, and saltbush shines dry grinding, 4 times of volumes of addition The ethanol that mass concentration is 75% soaks 5 minutes, is then extracted 35 minutes at 65 DEG C, filters, and residue extracts at 65 DEG C again 35 minutes, the extract solution merged twice can obtain saltbush extract solution.
Further illustrate, extra large silkweed uses extra large silkweed extract in the present invention, after extra large silkweed complete stool is shone into dry grinding The degreasing of progress soxhlet extraction, water extract-alcohol precipitation, centrifugation, water-bath are volatilized, are freeze-dried successively, produce extra large silkweed extract.
Further illustrate, kuh-seng uses Radix Sophorae Flavescentis extractive liquid in the present invention, and kuh-seng is added into volume ratio for 4 times of kuh-seng Mass concentration is to be ground 4 minutes in 75% ethanol, is then extracted the mixed liquor after grinding, circulating repetition 4 times, remember to obtain Radix Sophorae Flavescentis extractive liquid.
Further illustrate, Sophora alopecuroide uses Sophora alopecuroide extract solution in the present invention, is added after Sophora alopecuroide is cleaned appropriate Water is beaten, then add lauryl sodium sulfate soak 20 hours, then pressure be 0.85MPa, temperature be 35 DEG C of bars Cohobation 25 minutes is carried out under part, it is Sophora alopecuroide extract solution then to carry out press filtration and obtain filtrate.
Embodiment 2：
A kind of implantation methods for improving rice quality, the implantation methods of the raising rice quality are according to percent by weight bag Include 17% bagasse, 12% furfural dregs, 16% soybean slag, 7% phosphorus ore slag, 8% charcoal, 7% nitrate fertilizer, 5% humic acid, 4% chondrodite, 2.8% Cynanchum Komarrivii AI Iijiniski, 3.5% saltbush, 3.6% extra large silkweed, 2.6% kuh-seng, 3.6% Sophora alopecuroide, 2.6% yeast Bacterium, 1.7% nitric acid needle-like bacterium, 1.8% thread sulfur bacteria, 1.8% bacillus megaterium.
The raw material of aforementioned proportion is used to prepare a kind of method for the implantation methods for improving rice quality, including in detail below Step：
S1, by peptone 18g, brewer's wort 15g, beef extract 8g, glucose 15g, the agar (W/V) 1.2% of 7 degree of Baumes, 1000ml is added water to, saccharomycete is inoculated in fluid nutrient medium by pH6 as fluid nutrient medium, then in terms of v/v 5% inoculum concentration In fermented 20 hours under conditions of 25 DEG C of temperature, rotating speed 300rpm after can obtain effective bacteria containing amount as 2.15 × 108Individual/mL Saccharomycete；
S2, by NaHCO3 0.34g、NH4Cl 0.13g、MgSO4·7H2O 0.25g、K2HPO4 1.35g、Na(CH3COO) 1.85g、KNO3 1.25g、Na2S·9H2O 3g add water to 10ml, pH8 as fluid nutrient medium, inoculum concentration according to using v/v as 10% addition nitric acid needle-like bacterium cultivates 25h under conditions of 28 DEG C, you can obtains effective bacteria containing amount as 1.15 × 108Individual/mL's Nitric acid needle-like bacterium；
S3, by EDTA 12g, NaHCO3 15g、FeCl2·4H2O 10g、MnCl2·2H2O 20g、K2HPO4 1.35g、 (NH4)6Mo7O24·4H2O 16g、KNO3 15g、Na2S·9H2O 12g, agar 10g add water to 1000ml, pH8-8.5 conducts Fluid nutrient medium, inoculum concentration are that 10% plus thread sulfur bacteria cultivate 25-30h under conditions of 28 DEG C according to using v/v, you can are obtained Effective bacteria containing amount is 1.11 × 108Individual/mL thread sulfur bacteria；
S4, brewer's wort 1%, sodium chloride 3%, dipotassium hydrogen phosphate 0.3%, glucose 2%, iron chloride 0.5%, ammonium sulfate 0.02%th, magnesium sulfate 0.03%, dusty yeast 0.08%, agar 0.1%, calcium carbonate 0.1%, excess water polishing to 100%, configuration Into 1000ml fluid nutrient medium, inoculum concentration according to using v/v be 8% plus bacillus megaterium cultivate 25h under conditions of 30 DEG C, Effective bacteria containing amount be can obtain as 3.22 × 108Individual/mL bacillus megateriums；
S5, according to percent by weight, the saccharomycete that above-mentioned steps culture is obtained, nitric acid needle-like bacterium, thread sulfur bacteria, Bacillus megaterium builds heap with bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite Temperature is that 30 DEG C of fermentations can obtain base manure in 10 days；
S6, according to percent by weight, by the base manure of above-mentioned steps and Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide Mixing temperature can be improved the implantation methods of rice quality after being fermented 15 hours for 27 DEG C.
Further illustrate, Cynanchum Komarrivii AI Iijiniski uses Cynanchum Komarrivii AI Iijiniski refining liquid in the present invention, according to quality after Cynanchum Komarrivii AI Iijiniski is crushed Than for 1:10 ratios add water progress microwave abstracting and extract to obtain crude extract, are subsequently added into ethanol solution in standard atmospheric pressure, 80 Distilled at DEG C, removal slips out thing, and it is Cynanchum Komarrivii AI Iijiniski refining liquid to obtain residue.
Further illustrate, saltbush uses saltbush extract solution in the present invention, and saltbush shines dry grinding, 3 times of volumes of addition The ethanol that mass concentration is 75% soaks 5 minutes, is then extracted 35 minutes at 65 DEG C, filters, and residue extracts at 65 DEG C again 35 minutes, the extract solution merged twice can obtain saltbush extract solution.
Further illustrate, extra large silkweed uses extra large silkweed extract in the present invention, after extra large silkweed complete stool is shone into dry grinding The degreasing of progress soxhlet extraction, water extract-alcohol precipitation, centrifugation, water-bath are volatilized, are freeze-dried successively, produce extra large silkweed extract.
Further illustrate, kuh-seng uses Radix Sophorae Flavescentis extractive liquid in the present invention, is 3-5 times of kuh-seng by kuh-seng addition volume ratio Mass concentration be 75% ethanol in be ground 3 minutes, then the mixed liquor after grinding is extracted, circulation weight It is multiple 3-5 times, remember to obtain Radix Sophorae Flavescentis extractive liquid.
Further illustrate, Sophora alopecuroide uses Sophora alopecuroide extract solution in the present invention, is added after Sophora alopecuroide is cleaned appropriate Water is beaten, then add lauryl sodium sulfate soak 20 hours, then pressure be 0.8MPa, temperature be 30 DEG C of conditions Lower progress cohobation 20 minutes, it is Sophora alopecuroide extract solution then to carry out press filtration and obtain filtrate.
Embodiment 3：
A kind of implantation methods for improving rice quality, the implantation methods of the raising rice quality are according to percent by weight bag Include 20% bagasse, 15% furfural dregs, 20% soybean slag, 5% phosphorus ore slag, 5% charcoal, 10% nitrate fertilizer, 7% rotten plant Acid, 3% chondrodite, 2% Cynanchum Komarrivii AI Iijiniski, 1% saltbush, 1% extra large silkweed, 1% kuh-seng, 1% Sophora alopecuroide, 3% saccharomycete, 2% nitre Sour needle-like bacterium, 2% thread sulfur bacteria, 2% bacillus megaterium.
The raw material of aforementioned proportion is used to prepare a kind of method for the implantation methods for improving rice quality, including in detail below Step：
S1, by peptone 18g, brewer's wort 15g, beef extract 8g, glucose 15g, the agar (W/V) 1.2% of 7 degree of Baumes, 1000ml is added water to, saccharomycete is inoculated in Liquid Culture by pH6.5 as fluid nutrient medium, then in terms of v/v 5% inoculum concentration Effective bacteria containing amount is can obtain after being fermented 24 hours under conditions of 25 DEG C of temperature, rotating speed 300rpm in base as 3.21 × 108Individual/ ML saccharomycete；
S2, by NaHCO3 0.34g、NH4Cl 0.13g、MgSO4·7H2O 0.25g、K2HPO4 1.35g、Na(CH3COO) 1.85g、KNO3 1.25g、Na2S·9H2O 3g add water to 10ml, pH8.5 as fluid nutrient medium, inoculum concentration according to using v/v as 10% addition nitric acid needle-like bacterium cultivates 25-30h under conditions of 28 DEG C, you can obtains effective bacteria containing amount as 2.15 × 108Individual/mL Nitric acid needle-like bacterium；
S3, by EDTA 12g, NaHCO3 15g、FeCl2·4H2O 10g、MnCl2·2H2O 20g、K2HPO4 1.35g、 (NH4)6Mo7O24·4H2O 16g、KNO3 15g、Na2S·9H2O 12g, agar 10g add water to 1000ml, and pH8-8.5 is as liquid Body culture medium, inoculum concentration are that 10% plus thread sulfur bacteria cultivate 25-30h under conditions of 28 DEG C according to using v/v, you can are had It is 3.45 × 10 to imitate bacteria containing amount8Individual/mL thread sulfur bacteria；
S4, brewer's wort 1%, sodium chloride 3%, dipotassium hydrogen phosphate 0.3%, glucose 2%, iron chloride 0.5%, ammonium sulfate 0.02%th, magnesium sulfate 0.03%, dusty yeast 0.08%, agar 0.1%, calcium carbonate 0.1%, excess water polishing to 100%, configuration Into 1000ml fluid nutrient medium, inoculum concentration according to using v/v be 8% plus bacillus megaterium cultivate 30h under conditions of 30 DEG C, Effective bacteria containing amount be can obtain as 5.16 × 108Individual/mL bacillus megateriums；
S5, according to percent by weight, the saccharomycete that above-mentioned steps culture is obtained, nitric acid needle-like bacterium, thread sulfur bacteria, Bacillus megaterium builds heap with bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite Temperature is that 32 DEG C of fermentations can obtain base manure in 10 days；
S6, according to percent by weight, by the base manure of above-mentioned steps and Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide Mixing temperature can be improved the implantation methods of rice quality after being fermented 25 hours for 30 DEG C.
Further illustrate, Cynanchum Komarrivii AI Iijiniski uses Cynanchum Komarrivii AI Iijiniski refining liquid in the present invention, according to quality after Cynanchum Komarrivii AI Iijiniski is crushed Than for 1:10-20 ratios add water carry out microwave abstracting extract to obtain crude extract, be subsequently added into ethanol solution standard atmospheric pressure, Distilled at 90 DEG C, removal slips out thing, and it is Cynanchum Komarrivii AI Iijiniski refining liquid to obtain residue.
Further illustrate, saltbush uses saltbush extract solution in the present invention, and saltbush shines dry grinding, 5 times of volumes of addition The ethanol that mass concentration is 75% soaks 5 minutes, is then extracted 35 minutes at 65 DEG C, filters, and residue extracts at 65 DEG C again 35 minutes, the extract solution merged twice can obtain saltbush extract solution.
Further illustrate, extra large silkweed uses extra large silkweed extract in the present invention, after extra large silkweed complete stool is shone into dry grinding The degreasing of progress soxhlet extraction, water extract-alcohol precipitation, centrifugation, water-bath are volatilized, are freeze-dried successively, produce extra large silkweed extract.
Further illustrate, kuh-seng uses Radix Sophorae Flavescentis extractive liquid in the present invention, and kuh-seng is added into volume ratio for 5 times of kuh-seng Mass concentration is to be ground 5 minutes in 75% ethanol, is then extracted the mixed liquor after grinding, circulating repetition 5 times, remember to obtain Radix Sophorae Flavescentis extractive liquid.
Further illustrate, Sophora alopecuroide uses Sophora alopecuroide extract solution in the present invention, is added after Sophora alopecuroide is cleaned appropriate Water is beaten, then add lauryl sodium sulfate soak 20 hours, then pressure be 0.9MPa, temperature be 40 DEG C of conditions Lower progress cohobation 30 minutes, it is Sophora alopecuroide extract solution then to carry out press filtration and obtain filtrate.
Embodiment 4：
A kind of implantation methods for improving rice quality, the implantation methods of the raising rice quality are according to percent by weight bag Include 12% bagasse, 14% furfural dregs, 12.7% soybean slag, 9% phosphorus ore slag, 9% charcoal, 9% nitrate fertilizer, 7% rotten plant Acid, 4.5% chondrodite, 4.5% Cynanchum Komarrivii AI Iijiniski, 1.5% saltbush, 4.5% extra large silkweed, 1.5% kuh-seng, 1.5% Sophora alopecuroide, 2.9% saccharomycete, 1.2% nitric acid needle-like bacterium, 1.9% thread sulfur bacteria, 1.3% bacillus megaterium.
The raw material of aforementioned proportion is used to prepare a kind of method for the implantation methods for improving rice quality, including in detail below Step：
S1, by peptone 18g, brewer's wort 15g, beef extract 8g, glucose 15g, the agar (W/V) 1.2% of 7 degree of Baumes, 1000ml is added water to, saccharomycete is inoculated in Liquid Culture by pH6.3 as fluid nutrient medium, then in terms of v/v 5% inoculum concentration Effective bacteria containing amount is can obtain after being fermented 23 hours under conditions of 25 DEG C of temperature, rotating speed 300rpm in base as 2.88 × 108Individual/ ML saccharomycete；
S2, by NaHCO3 0.34g、NH4Cl 0.13g、MgSO4·7H2O 0.25g、K2HPO4 1.35g、Na(CH3COO) 1.85g、KNO3 1.25g、Na2S·9H2O 3g add water to 10ml, pH8.2 as fluid nutrient medium, inoculum concentration according to using v/v as 10% addition nitric acid needle-like bacterium cultivates 26h under conditions of 28 DEG C, you can obtains effective bacteria containing amount as 1.26 × 108Individual/mL's Nitric acid needle-like bacterium；
S3, by EDTA 12g, NaHCO3 15g、FeCl2·4H2O 10g、MnCl2·2H2O 20g、K2HPO4 1.35g、 (NH4)6Mo7O24·4H2O 16g、KNO3 15g、Na2S·9H2O 12g, agar 10g add water to 1000ml, and pH8 is as liquid Culture medium, inoculum concentration are that 10% plus thread sulfur bacteria cultivate 29h under conditions of 28 DEG C according to using v/v, you can are effectively contained Bacterium amount is 3.02 × 108Individual/mL thread sulfur bacteria；
S4, brewer's wort 1%, sodium chloride 3%, dipotassium hydrogen phosphate 0.3%, glucose 2%, iron chloride 0.5%, ammonium sulfate 0.02%th, magnesium sulfate 0.03%, dusty yeast 0.08%, agar 0.1%, calcium carbonate 0.1%, excess water polishing to 100%, configuration Into 1000ml fluid nutrient medium, inoculum concentration according to using v/v be 8% plus bacillus megaterium cultivate 29h under conditions of 30 DEG C, Effective bacteria containing amount be can obtain as 4.89 × 108Individual/mL bacillus megateriums；
S5, according to percent by weight, the saccharomycete that above-mentioned steps culture is obtained, nitric acid needle-like bacterium, thread sulfur bacteria, Bacillus megaterium builds heap with bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite Temperature is that 30-32 DEG C of fermentation can obtain base manure in 10 days；
S6, according to percent by weight, by the base manure of above-mentioned steps and Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide Mixing temperature can be improved the implantation methods of rice quality after being fermented 22 hours for 29 DEG C.
Further illustrate, Cynanchum Komarrivii AI Iijiniski uses Cynanchum Komarrivii AI Iijiniski refining liquid in the present invention, according to quality after Cynanchum Komarrivii AI Iijiniski is crushed Than for 1:18 ratios add water progress microwave abstracting and extract to obtain crude extract, are subsequently added into ethanol solution in standard atmospheric pressure, 88 Distilled at DEG C, removal slips out thing, and it is Cynanchum Komarrivii AI Iijiniski refining liquid to obtain residue.
Further illustrate, saltbush uses saltbush extract solution in the present invention, and saltbush shines dry grinding, 5 times of volumes of addition The ethanol that mass concentration is 75% soaks 5 minutes, is then extracted 35 minutes at 65 DEG C, filters, and residue extracts at 65 DEG C again 35 minutes, the extract solution merged twice can obtain saltbush extract solution.
Further illustrate, extra large silkweed uses extra large silkweed extract in the present invention, after extra large silkweed complete stool is shone into dry grinding The degreasing of progress soxhlet extraction, water extract-alcohol precipitation, centrifugation, water-bath are volatilized, are freeze-dried successively, produce extra large silkweed extract.
Further illustrate, kuh-seng uses Radix Sophorae Flavescentis extractive liquid in the present invention, and kuh-seng is added into volume ratio for 4 times of kuh-seng Mass concentration is to be ground 5 minutes in 75% ethanol, is then extracted the mixed liquor after grinding, circulating repetition 4 times, remember to obtain Radix Sophorae Flavescentis extractive liquid.
Further illustrate, Sophora alopecuroide uses Sophora alopecuroide extract solution in the present invention, is added after Sophora alopecuroide is cleaned appropriate Water is beaten, then add lauryl sodium sulfate soak 20 hours, then pressure be 0.8MPa, temperature be 34 DEG C of conditions Lower progress cohobation 27 minutes, it is Sophora alopecuroide extract solution then to carry out press filtration and obtain filtrate.
Embodiment 5：
A kind of implantation methods for improving rice quality, the implantation methods of the raising rice quality are according to percent by weight bag Include 18% bagasse, 14% furfural dregs, 17.4% soybean slag, 6% phosphorus ore slag, 6% charcoal, 6% nitrate fertilizer, 3% rotten plant Acid, 3.5% chondrodite, 2.5% Cynanchum Komarrivii AI Iijiniski, 4.5% saltbush, 2% extra large silkweed, 4.5% kuh-seng, 4.5% Sophora alopecuroide, 2.5% Saccharomycete, 1.9% nitric acid needle-like bacterium, 1.1% thread sulfur bacteria, 2.6% bacillus megaterium.
The raw material of aforementioned proportion is used to prepare a kind of method for the implantation methods for improving rice quality, including in detail below Step：
S1, by peptone 18g, brewer's wort 15g, beef extract 8g, glucose 15g, the agar (W/V) 1.2% of 7 degree of Baumes, 1000ml is added water to, saccharomycete is inoculated in Liquid Culture by pH6.4 as fluid nutrient medium, then in terms of v/v 5% inoculum concentration Effective bacteria containing amount is can obtain after being fermented 23 hours under conditions of 25 DEG C of temperature, rotating speed 300rpm in base as 3.11 × 108Individual/ ML saccharomycete；
S2, by NaHCO3 0.34g、NH4Cl 0.13g、MgSO4·7H2O 0.25g、K2HPO4 1.35g、Na(CH3COO) 1.85g、KNO3 1.25g、Na2S·9H2O 3g add water to 10ml, pH8.4 as fluid nutrient medium, inoculum concentration according to using v/v as 10% addition nitric acid needle-like bacterium cultivates 27h under conditions of 28 DEG C, you can obtains effective bacteria containing amount as 1.74 × 108Individual/mL's Nitric acid needle-like bacterium；
S3, by EDTA 12g, NaHCO3 15g、FeCl2·4H2O 10g、MnCl2·2H2O 20g、K2HPO4 1.35g、 (NH4)6Mo7O24·4H2O 16g、KNO3 15g、Na2S·9H2O 12g, agar 10g add water to 1000ml, and pH8.1 is as liquid Body culture medium, inoculum concentration are that 10% plus thread sulfur bacteria cultivate 27h under conditions of 28 DEG C according to using v/v, you can are obtained effectively Bacteria containing amount is 2.11 × 108Individual/mL thread sulfur bacteria；
S4, brewer's wort 1%, sodium chloride 3%, dipotassium hydrogen phosphate 0.3%, glucose 2%, iron chloride 0.5%, ammonium sulfate 0.02%th, magnesium sulfate 0.03%, dusty yeast 0.08%, agar 0.1%, calcium carbonate 0.1%, excess water polishing to 100%, configuration Into 1000ml fluid nutrient medium, inoculum concentration according to using v/v be 8% plus bacillus megaterium cultivate 26h under conditions of 30 DEG C, Effective bacteria containing amount be can obtain as 3.59 × 108Individual/mL bacillus megateriums；
S5, according to percent by weight, the saccharomycete that above-mentioned steps culture is obtained, nitric acid needle-like bacterium, thread sulfur bacteria, Bacillus megaterium builds heap with bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite Temperature is that 31 DEG C of fermentations can obtain base manure in 10 days；
S6, according to percent by weight, by the base manure of above-mentioned steps and Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide Mixing temperature can be improved the implantation methods of rice quality after being fermented 23 hours for 28 DEG C.
Further illustrate, Cynanchum Komarrivii AI Iijiniski uses Cynanchum Komarrivii AI Iijiniski refining liquid in the present invention, according to quality after Cynanchum Komarrivii AI Iijiniski is crushed Than for 1:16 ratios add water progress microwave abstracting and extract to obtain crude extract, are subsequently added into ethanol solution in standard atmospheric pressure, 87 Distilled at DEG C, removal slips out thing, and it is Cynanchum Komarrivii AI Iijiniski refining liquid to obtain residue.
Further illustrate, saltbush uses saltbush extract solution in the present invention, and saltbush shines dry grinding, 3 times of volumes of addition The ethanol that mass concentration is 75% soaks 5 minutes, is then extracted 35 minutes at 65 DEG C, filters, and residue extracts at 65 DEG C again 35 minutes, the extract solution merged twice can obtain saltbush extract solution.
Further illustrate, extra large silkweed uses extra large silkweed extract in the present invention, after extra large silkweed complete stool is shone into dry grinding The degreasing of progress soxhlet extraction, water extract-alcohol precipitation, centrifugation, water-bath are volatilized, are freeze-dried successively, produce extra large silkweed extract.
Further illustrate, kuh-seng uses Radix Sophorae Flavescentis extractive liquid in the present invention, and kuh-seng is added into volume ratio for 5 times of kuh-seng Mass concentration is to be ground 4 minutes in 75% ethanol, is then extracted the mixed liquor after grinding, circulating repetition 4 times, remember to obtain Radix Sophorae Flavescentis extractive liquid.
Further illustrate, Sophora alopecuroide uses Sophora alopecuroide extract solution in the present invention, is added after Sophora alopecuroide is cleaned appropriate Water is beaten, then add lauryl sodium sulfate soak 20 hours, then pressure be 0.85MPa, temperature be 37 DEG C of bars Cohobation 27 minutes is carried out under part, it is Sophora alopecuroide extract solution then to carry out press filtration and obtain filtrate.
Testing inspection data explanation：
Control group 1：Same as Example 5, difference is to be not added with Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide；
Control group 2：Same as Example 5, difference is to be not added with soybean slag；
Embodiments of the invention 1-5, control group the 1-2 rice shoot for cultivating to obtain are subjected to conventional rice and are colonized plantation, it is right Following indexs are detected：
Maturity period, each cell singles singly receive, and paddy claims dry mass and determines moisture after drying, then converse aqueous Measure the rice yield for 14%.While surveying production, each cell randomly selects 6 root and stem of certain plants plant and gathers in, air-dries together, divides paddy and straw Weigh respectively, calculate ratio of grain to straw, then according to ratio of grain to straw conversion straw yield.
The rice dry matter amount situation of table 1 (average ± standard deviation n=3)
The different letters of each numerical value represent the significance of difference (P in upper table<0.05)
By upper table data analysis understand embodiment 1-5 production rice quality it is more preferable than control group 1-2, embodiment it is total Amount of dry matter is high, and harvest index is high, and because resistance against diseases is poor in comparative example 1, generation disease, which reaches, to yield poorly, in comparative example 2 Fertilizer is acid to be unfavorable for greatly seedling growth, and yield is compared and also reduced after caused Seedlings quality steps into embodiment 1-5 therefore is colonized.
Millet after embodiment 1-5, control group 1-2 kinds are planted, which picks and places, puts more than January, takes 1kg to determine quality.Coarse Rice Rate, Polished rice rate, head rice rate, chalky grain rate, Chalkiness size, chalkiness degree and paddy length-width ratio are with reference to country of People's Republic of China (PRC) mark It is accurate《GB/T17891-1999 high quality paddies》Measure；Brown rice protein, brown rice amylose, polished rice protein and polished rice straight chain form sediment Powder content is determined using near-infrared grain analyzer (production of FOSS companies of Sweden)
The rice quality situation table of table 2
The different letters of each numerical value represent the significance of difference (P in upper table<0.05)
Rice paddy after the rice shoot field planting obtained as seen from the above table using the implantation methods of the raising rice quality of the application Sub- quality is more preferable.
The rice shoot that the implantation methods culture of rice quality is improved using the application has good resistance against diseases, so as to be colonized Resistance is strong behind crop field, and generation pest and disease damage probability is small (the probability statistics that disease occurs see the table below), and survival rate is high, and increase income One key factor, and the probability that pest and disease damage occurs is few, and a major reason increased income.
The disease rate situation table disease rate % of table 3
Remarks：Disease rate %=onset areas/cultivated area
The probability that disease occurs using the culture medium of the application as seen from the above table is far smaller than control group.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims (7)

  1. A kind of 1. implantation methods for improving rice quality, it is characterised in that：Comprise the following steps：
    (1), choose seeds：After the seed of full grains is dried immersion one day is carried out using water；
    (2), seedling planting：Good seed is sowed on seedling soil after going out, and then spreads the middle sand of 1-2 centimetres of last layer, is kept The humidity of seedling soil is in 60-75%, when rice shoot length is to 10-15 centimetres, waits to be colonized in big Tanaka；
    (3), Field planting：The rice shoot rice transplanting that seedling obtains is colonized in big Tanaka, spacing in the rows is 15-20 centimetres of 15-20 cm x, Period keeps the humidity in crop field to carry out conventional top dressing, desinsection and weeding management during field planting in 70-75%, until harvesting；
    The seedling soil is big including 10%-20% bagasse, 8%-15% furfural dregs, 10%-20% according to percent by weight Bean dregs, 5%-10% phosphorus ores slag, 5%-10% charcoals, 5%-10% nitrate fertilizers, 2%-8% humic acids, 3%-5% grain silicon Magnesite, 2%-5% Cynanchum Komarrivii AI Iijiniskis, 1%-5% saltbush, 1%-5% seas silkweed, 1%-5% kuh-sengs, 1%-5% Sophora alopecuroides, 2%- 3% saccharomycete, 1%-2% nitric acid needle-likes bacterium, the thread sulfur bacterias of 1%-2%, 1%-2% bacillus megateriums.
  2. A kind of 2. implantation methods for improving rice quality according to claim 1, it is characterised in that：The seedling soil according to Percent by weight includes 12%-18% bagasse, 10%-14% furfural dregs, 12%-18% soybean slags, 6%-9% phosphorus ores Slag, 6%-9% charcoals, 6%-9% nitrate fertilizers, 3%-7% humic acids, 3.5%-4.5% chondrodites, 2.5%- 4.5% Cynanchum Komarrivii AI Iijiniski, 1.5%-4.5% saltbush, 2%-4.5% seas silkweed, 1.5%-4.5% kuh-sengs, 1.5%-4.5% hardship beans Son, 2.5%-2.9% saccharomycete, 1.2%-1.9% nitric acid needle-likes bacterium, the thread sulfur bacterias of 1.1%-1.9%, 1.3%-2.6% Bacillus megaterium.
  3. A kind of 3. implantation methods for improving rice quality according to claim 1, it is characterised in that：The seedling soil according to Percent by weight includes 17% bagasse, 12% furfural dregs, 16% soybean slag, 7% phosphorus ore slag, 8% charcoal, 7% nitre state Nitrogenous fertilizer, 5% humic acid, 4% chondrodite, 2.8% Cynanchum Komarrivii AI Iijiniski, 3.5% saltbush, 3.6% extra large silkweed, 2.6% kuh-seng, 3.6% Sophora alopecuroide, 2.6% saccharomycete, 1.7% nitric acid needle-like bacterium, 1.8% thread sulfur bacteria, 1.8% bacillus megaterium.
  4. A kind of 4. implantation methods for improving rice quality according to claim 1, it is characterised in that：The saccharomycete is effective Bacteria containing amount is 2.15 × 108-3.21×108Individual/mL, the effective bacteria containing amount of nitric acid needle-like bacterium are 1.15 × 108-2.15×108Individual/ ML, the effective bacteria containing amount of thread sulfur bacteria are 1.11 × 108-3.45×108The effective bacteria containing amount of individual/mL, bacillus megaterium is 3.22 ×108-5.16×108Individual/mL.
  5. 5. a kind of preparation method of the implantation methods of raising rice quality according to claim 1-4 any one, it is special Sign is：Including step in detail below：
    S1, by peptone 18g, brewer's wort 15g, beef extract 8g, glucose 15g, the agar (W/V) 1.2% of 7 degree of Baumes, add water Saccharomycete is inoculated in fluid nutrient medium as fluid nutrient medium, then in terms of v/v 5% inoculum concentration to 1000ml, pH6-6.5 In ferment under conditions of 25 DEG C of the temperature, rotating speed 300rpm and can obtain effective bacteria containing amount as 2.15 × 10 after 20-24 hours8- 3.21×108Individual/mL saccharomycete；
    S2, by NaHCO3 0.34g、NH4Cl 0.13g、MgSO4·7H2O 0.25g、K2HPO4 1.35g、Na(CH3COO) 1.85g、KNO3 1.25g、Na2S·9H2O 3g add water to 10ml, and pH8-8.5 is as fluid nutrient medium, and inoculum concentration is according to v/v 25-30h is cultivated under conditions of 28 DEG C, you can obtain effective bacteria containing amount as 1.15 × 10 for 10% addition nitric acid needle-like bacterium8- 2.15×108Individual/mL nitric acid needle-like bacterium；
    S3, by EDTA 12g, NaHCO3 15g、FeCl2·4H2O 10g、MnCl2·2H2O 20g、K2HPO4 1.35g、 (NH4)6Mo7O24·4H2O 16g、KNO3 15g、Na2S·9H2O 12g, agar 10g add water to 1000ml, pH8-8.5 conducts Fluid nutrient medium, inoculum concentration are that 10% plus thread sulfur bacteria cultivate 25-30h under conditions of 28 DEG C according to using v/v, you can are obtained Effective bacteria containing amount is 1.11 × 108-3.45×108Individual/mL thread sulfur bacteria；
    S4, brewer's wort 1%, sodium chloride 3%, dipotassium hydrogen phosphate 0.3%, glucose 2%, iron chloride 0.5%, ammonium sulfate 0.02%th, magnesium sulfate 0.03%, dusty yeast 0.08%, agar 0.1%, calcium carbonate 0.1%, excess water polishing to 100%, configuration Into 1000ml fluid nutrient medium, inoculum concentration is that 8% plus bacillus megaterium cultivate 25- under conditions of 30 DEG C according to using v/v 30h, you can obtain effective bacteria containing amount as 3.22 × 108-5.16×108Individual/mL bacillus megateriums；
    S5, according to percent by weight, it is the saccharomycete that above-mentioned steps culture is obtained, nitric acid needle-like bacterium, thread sulfur bacteria, huge Bacillus builds heap fermentation with bagasse, furfural dregs, soybean slag, phosphorus ore slag, charcoal, nitrate fertilizer, humic acid, chondrodite It can obtain base manure within 10 days；
    S6, according to percent by weight, the base manure of above-mentioned steps is mixed with Cynanchum Komarrivii AI Iijiniski, saltbush, extra large silkweed, kuh-seng, Sophora alopecuroide The implantation methods of rice quality can be improved after fermentation 15-25 hours.
  6. A kind of 6. preparation method of implantation methods for improving rice quality according to claim 5, it is characterised in that：It is described The fermentation condition of S5 steps is that temperature is 30-32 DEG C.
  7. A kind of 7. preparation method of implantation methods for improving rice quality according to claim 5, it is characterised in that：It is described The fermentation condition of S6 steps is that temperature is 27-30 DEG C.
CN201710641831.0A 2017-07-31 2017-07-31 A kind of implantation methods for improving rice quality and preparation method thereof Pending CN107466751A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710641831.0A CN107466751A (en) 2017-07-31 2017-07-31 A kind of implantation methods for improving rice quality and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710641831.0A CN107466751A (en) 2017-07-31 2017-07-31 A kind of implantation methods for improving rice quality and preparation method thereof

Publications (1)

Publication Number Publication Date
CN107466751A true CN107466751A (en) 2017-12-15

Family

ID=60598011

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710641831.0A Pending CN107466751A (en) 2017-07-31 2017-07-31 A kind of implantation methods for improving rice quality and preparation method thereof

Country Status (1)

Country Link
CN (1) CN107466751A (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101563995A (en) * 2008-04-25 2009-10-28 林天国 Planting method of paddy rice by water-free arid soil transplantation
CN104381064A (en) * 2014-09-24 2015-03-04 江苏农林职业技术学院 Paddy rice planting method
CN105123330A (en) * 2015-08-31 2015-12-09 潍坊友容实业有限公司 Seedling culturing substrate containing Chinese medicine residues and application of seedling culturing substrate in salt golden cypress bonsai seedling culturing
CN105145246A (en) * 2015-08-31 2015-12-16 潍坊友容实业有限公司 Method for seedling culture of high-survival-rate tamarix chinensis bonsai
CN105732195A (en) * 2016-02-01 2016-07-06 广西微力大生物科技有限公司 Composite microbial fertilizer for controlling soil-borne disease and preparation method thereof
CN106171633A (en) * 2016-07-11 2016-12-07 全椒县志恒庄园 A kind of paddy drought seedling bed soil
CN106305196A (en) * 2016-07-29 2017-01-11 宝兴县淡蓝种植专业合作社 Special culture soil for breeding seedlings of bletilla striata and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101563995A (en) * 2008-04-25 2009-10-28 林天国 Planting method of paddy rice by water-free arid soil transplantation
CN104381064A (en) * 2014-09-24 2015-03-04 江苏农林职业技术学院 Paddy rice planting method
CN105123330A (en) * 2015-08-31 2015-12-09 潍坊友容实业有限公司 Seedling culturing substrate containing Chinese medicine residues and application of seedling culturing substrate in salt golden cypress bonsai seedling culturing
CN105145246A (en) * 2015-08-31 2015-12-16 潍坊友容实业有限公司 Method for seedling culture of high-survival-rate tamarix chinensis bonsai
CN105732195A (en) * 2016-02-01 2016-07-06 广西微力大生物科技有限公司 Composite microbial fertilizer for controlling soil-borne disease and preparation method thereof
CN106171633A (en) * 2016-07-11 2016-12-07 全椒县志恒庄园 A kind of paddy drought seedling bed soil
CN106305196A (en) * 2016-07-29 2017-01-11 宝兴县淡蓝种植专业合作社 Special culture soil for breeding seedlings of bletilla striata and preparation method thereof

Similar Documents

Publication Publication Date Title
CN102876576B (en) Acidic soil conditioner, and preparation method and application thereof
CN104609997A (en) Manufacturing process and application method of ginseng bio-organic fertilizer
CN103214292A (en) Plant cultivation medium and its preparation method
CN107580818B (en) A kind of integrated approach of soil conditioning and reparation
CN107723258B (en) Composite microbial inoculum for preventing and treating tobacco bacterial wilt and preparation method thereof
CN109852565A (en) A kind of salt-soda soil composite modifying agent and its method of administration
CN106699332A (en) Production method of special fertilizer for watermelon
CN108821873A (en) Rice hull carbon base manure material and preparation method thereof and its application in Rice Cropping
CN104788237B (en) A kind of wheat selenium-rich zinc-iron microbial fermentation fertilizer and its preparation method and application
CN103964951B (en) A kind of magnetization complex microorganism organic water-soluble fertilizer and its preparation method and application
CN107324940A (en) A kind of dragon fruit synergy fertilizer and preparation method thereof
CN111411063A (en) Bacillus composite microbial inoculum with function of preventing and treating tobacco soil-borne diseases and preparation method thereof
CN110330370A (en) While a kind of turf biological organic fertilizer and its preparation method and application containing trichoderma and bacillus amyloliquefaciens
CN107188749A (en) A kind of high-yield planting base manure of passion fruit and preparation method thereof
CN108676755A (en) A kind of microbial liquid fertilizer and its preparation method and application containing bacillus
CN107338056B (en) Green microbial soil conditioner and preparation method thereof
CN109355197B (en) Growth-promoting bacterium for promoting growth of saline-alkali soil alfalfa and microbial organic fertilizer thereof
CN105175162B (en) It is a kind of for preventing and treating the microbial organic fertilizer and its preparation and application of bacterial wilt
CN101870595B (en) Old ginseng land soil biological modifying agent
CN105802898A (en) Bacillus amyloliquefaciens strain and biological organic fertilizer
Giöbel The relation of the soil nitrogen to nodule development and fixation of nitrogen by certain legumes
CN101569284A (en) Preparation method of mycorrhizal tobacco seedling by taking sand as ground substance
CN105347860A (en) Pome organic fertilizer
CN106396861A (en) Compound microbial bamboo shoot fertilizer and preparation method thereof
CN110713953A (en) Mesorhizobium strain with phosphate solubilizing property and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20171215