CN111808276A - 制备用于细胞培养基中的泊洛沙姆的方法 - Google Patents

制备用于细胞培养基中的泊洛沙姆的方法 Download PDF

Info

Publication number
CN111808276A
CN111808276A CN202010723304.6A CN202010723304A CN111808276A CN 111808276 A CN111808276 A CN 111808276A CN 202010723304 A CN202010723304 A CN 202010723304A CN 111808276 A CN111808276 A CN 111808276A
Authority
CN
China
Prior art keywords
poloxamer
minutes
cell culture
cell
heated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010723304.6A
Other languages
English (en)
Chinese (zh)
Inventor
G·P·维瑟维斯瓦兰
R·D·基斯
S·J·迈耶
I·权
K·卡尔霍恩
K·温彻斯特
A·亚当斯
M·格伦
S·凯尼格
A·德泽
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN111808276A publication Critical patent/CN111808276A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
    • C08G65/30—Post-polymerisation treatment, e.g. recovery, purification, drying
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/34—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from hydroxy compounds or their metallic derivatives
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/34—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from hydroxy compounds or their metallic derivatives
    • C08G65/48—Polymers modified by chemical after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2650/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G2650/28—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type
    • C08G2650/50—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type containing nitrogen, e.g. polyetheramines or Jeffamines(r)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2650/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G2650/28—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type
    • C08G2650/58—Ethylene oxide or propylene oxide copolymers, e.g. pluronics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Peptides Or Proteins (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202010723304.6A 2014-03-25 2015-03-25 制备用于细胞培养基中的泊洛沙姆的方法 Pending CN111808276A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461970281P 2014-03-25 2014-03-25
US61/970,281 2014-03-25
CN201580026701.5A CN106414552B (zh) 2014-03-25 2015-03-25 制备用于细胞培养基中的泊洛沙姆的方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201580026701.5A Division CN106414552B (zh) 2014-03-25 2015-03-25 制备用于细胞培养基中的泊洛沙姆的方法

Publications (1)

Publication Number Publication Date
CN111808276A true CN111808276A (zh) 2020-10-23

Family

ID=52824596

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202010723304.6A Pending CN111808276A (zh) 2014-03-25 2015-03-25 制备用于细胞培养基中的泊洛沙姆的方法
CN201580026701.5A Active CN106414552B (zh) 2014-03-25 2015-03-25 制备用于细胞培养基中的泊洛沙姆的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201580026701.5A Active CN106414552B (zh) 2014-03-25 2015-03-25 制备用于细胞培养基中的泊洛沙姆的方法

Country Status (19)

Country Link
US (2) US11034793B2 (https=)
EP (2) EP3778702B1 (https=)
JP (3) JP6655548B2 (https=)
KR (1) KR102352250B1 (https=)
CN (2) CN111808276A (https=)
AU (1) AU2015236008B2 (https=)
BR (1) BR112016021739B1 (https=)
CA (1) CA2943357C (https=)
ES (1) ES2778680T3 (https=)
HR (1) HRP20200372T1 (https=)
IL (2) IL247902B (https=)
MX (2) MX2016012288A (https=)
MY (1) MY178060A (https=)
PL (1) PL3122799T3 (https=)
RU (1) RU2710551C2 (https=)
SG (1) SG11201607929PA (https=)
SI (1) SI3122799T1 (https=)
WO (1) WO2015148736A1 (https=)
ZA (1) ZA201606711B (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HRP20200372T1 (hr) 2014-03-25 2020-06-12 F. Hoffmann - La Roche Ag Postupci proizvodnje poloksamera za upotrebu u mediju za staničnu kulturu
EP3430072B1 (en) * 2016-03-17 2020-07-01 Merck Patent GmbH Method for purifying poloxamers
EP3728471A1 (en) * 2017-12-21 2020-10-28 Sigma Aldrich Co. LLC Poloxamer compositions and methods of making and using same
EP3790597A4 (en) * 2018-07-12 2022-03-09 Arshintseva, Elena Valentinovna THERMAL PROCESS FOR THE STERILIZATION OF POLOXAMER WITH LIQUID PHARMACEUTICALS
CN112469814A (zh) * 2018-07-27 2021-03-09 味之素株式会社 动物细胞的悬浮培养用添加物、悬浮培养用培养基和悬浮培养方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1753949A (zh) * 2003-02-27 2006-03-29 诺瓦赛澳大利亚有限公司 泊洛沙姆乳液制剂
US20060115535A1 (en) * 2004-11-30 2006-06-01 Basf Corporation Method for formation of micro-prilled polymers
CN1939534A (zh) * 2005-09-27 2007-04-04 长春金赛药业有限责任公司 含有人生长激素或人粒细胞巨噬细胞刺激因子的用于治疗损伤和溃疡的外用制剂

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE266710C (https=)
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
ATE135397T1 (de) * 1988-09-23 1996-03-15 Cetus Oncology Corp Zellenzuchtmedium für erhöhtes zellenwachstum, zur erhöhung der langlebigkeit und expression der produkte
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
FR2646437B1 (fr) 1989-04-28 1991-08-30 Transgene Sa Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
EP0402226A1 (en) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
DE69120146T2 (de) 1990-01-12 1996-12-12 Cell Genesys Inc Erzeugung xenogener antikörper
WO1996033735A1 (en) 1995-04-27 1996-10-31 Abgenix, Inc. Human antibodies derived from immunized xenomice
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
ATE158021T1 (de) 1990-08-29 1997-09-15 Genpharm Int Produktion und nützung nicht-menschliche transgentiere zur produktion heterologe antikörper
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
BR9206593A (pt) 1991-10-04 1995-11-28 Gs Biochem Ab Partículas especialmente partículas coloidais processo de prepará-las a partir de uma fase cristalina líquida homogênea ou fase L3 composição farmacêutica e uso destas partículas
ATE219684T1 (de) * 1994-01-14 2002-07-15 Xoma Technology Ltd Anti gram positive bakterielle verfahren und mittel
US5912228A (en) * 1995-01-13 1999-06-15 Xoma Corporation Therapeutic compositions comprising bactericidal/permeability-increasing (BPI) protein products
WO1996034096A1 (en) 1995-04-28 1996-10-31 Abgenix, Inc. Human antibodies derived from immunized xenomice
CA2722378C (en) 1996-12-03 2015-02-03 Amgen Fremont Inc. Human antibodies that bind tnf.alpha.
GB9625175D0 (en) * 1996-12-04 1997-01-22 Medi Cult As Serum-free cell culture media
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
ES2248127T3 (es) 1999-10-04 2006-03-16 Medicago Inc. Metodo para regular la transcripcion de genes foraneos en presencia de nigtrogeno.
EP1596767B1 (en) * 2003-02-12 2018-10-03 Syncera, Inc. Random and non-random alkylene oxide polymer alloy compositions
US8802116B2 (en) * 2003-02-27 2014-08-12 Novasel Australia Pty. Ltd. Poloxamer emulsion preparations
WO2005014162A1 (en) 2003-08-04 2005-02-17 Camurus Ab Method for improving the properties of amphiphile particles
WO2005053652A1 (en) 2003-12-04 2005-06-16 Pfizer Products Inc. Multiparticulate crystalline drug compositions containing a poloxamer and a glyceride
EP1809272A4 (en) 2004-10-18 2008-01-02 Maroon Biotech Corp METHOD AND COMPOSITIONS FOR TREATING DAMAGES THROUGH FREE RADICALS
JP5546860B2 (ja) * 2006-08-16 2014-07-09 ノバルティス アーゲー 高結晶性治療化合物の固体分散体を製造するための方法
KR100932613B1 (ko) 2007-04-27 2009-12-17 한남대학교 산학협력단 고분자 용융공정을 이용한 약물전달용 생체적합성 고분자나노 미립구의 제조방법 및 그 나노 미립구
WO2009055312A1 (en) * 2007-10-22 2009-04-30 Dfb Pharmaceuticals, Inc. Process for producing a poloxamer gel
SG187397A1 (en) 2007-12-27 2013-02-28 Baxter Int Cell culture processes
CN102782121A (zh) * 2009-12-23 2012-11-14 赛诺菲巴斯德有限公司 用于培养贴壁细胞的方法
US9629811B2 (en) * 2012-03-30 2017-04-25 Institut National De La Sante Et De La Recherche Medicale (Inserm) Microsphere compositions, preparation method and applications thereof
HRP20200372T1 (hr) 2014-03-25 2020-06-12 F. Hoffmann - La Roche Ag Postupci proizvodnje poloksamera za upotrebu u mediju za staničnu kulturu

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1753949A (zh) * 2003-02-27 2006-03-29 诺瓦赛澳大利亚有限公司 泊洛沙姆乳液制剂
US20060115535A1 (en) * 2004-11-30 2006-06-01 Basf Corporation Method for formation of micro-prilled polymers
CN1939534A (zh) * 2005-09-27 2007-04-04 长春金赛药业有限责任公司 含有人生长激素或人粒细胞巨噬细胞刺激因子的用于治疗损伤和溃疡的外用制剂

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHUL SOON YONG，ET AL: "Enhanced anti-tumor activity and alleviated hepatotoxicity of clotrimazole-loaded suppository using poloxamer-propylene glycol gel", 《INTERNATIONAL JOURNAL OF PHARMACEUTICS》 *

Also Published As

Publication number Publication date
EP3778702A1 (en) 2021-02-17
BR112016021739A2 (pt) 2017-08-15
SG11201607929PA (en) 2016-10-28
SI3122799T1 (sl) 2020-06-30
KR102352250B1 (ko) 2022-01-17
NZ725151A (en) 2021-05-28
IL247902B (en) 2021-01-31
JP2023011566A (ja) 2023-01-24
CA2943357C (en) 2023-06-27
CA2943357A1 (en) 2015-10-01
EP3122799A1 (en) 2017-02-01
IL279583A (en) 2021-03-01
US20170009015A1 (en) 2017-01-12
RU2710551C2 (ru) 2019-12-27
ES2778680T3 (es) 2020-08-11
US11034793B2 (en) 2021-06-15
EP3122799B8 (en) 2020-03-11
WO2015148736A1 (en) 2015-10-01
RU2016141568A3 (https=) 2018-09-26
RU2016141568A (ru) 2018-04-28
EP3778702B1 (en) 2023-06-28
JP6655548B2 (ja) 2020-02-26
AU2015236008B2 (en) 2018-12-20
MX2021001981A (es) 2021-04-28
HRP20200372T1 (hr) 2020-06-12
KR20160138161A (ko) 2016-12-02
JP7472222B2 (ja) 2024-04-22
MY178060A (en) 2020-09-30
EP3122799B1 (en) 2020-01-15
BR112016021739B1 (pt) 2021-11-03
WO2015148736A8 (en) 2016-08-18
US11912823B2 (en) 2024-02-27
US20210371587A1 (en) 2021-12-02
CN106414552B (zh) 2020-08-25
JP2020103290A (ja) 2020-07-09
IL247902A0 (en) 2016-11-30
JP2017516459A (ja) 2017-06-22
ZA201606711B (en) 2019-12-18
AU2015236008A1 (en) 2016-10-27
CN106414552A (zh) 2017-02-15
IL279583B (en) 2022-03-01
PL3122799T3 (pl) 2020-06-29
MX2016012288A (es) 2017-01-23

Similar Documents

Publication Publication Date Title
JP7472222B2 (ja) 細胞培養培地における使用のためのポロキサマーを調製する方法
JP7377316B2 (ja) 哺乳類細胞培養物を回収するための方法
JP6591395B2 (ja) 抗酸化剤を含む細胞培養組成物およびポリペプチド産生のための方法
JP7075927B2 (ja) 緩衝剤溶液を提供するための方法及びシステム
EP3378932A1 (en) Serum-free cell culture medium
JP2011500054A (ja) 安定な保存用中間体を使用した生物製剤の製造方法
HK40038241A (en) Methods of preparing a poloxamer for use in cell culture medium
HK1233287B (zh) 制备用於细胞培养基中的泊洛沙姆的方法
HK1233287A1 (en) Methods of preparing a poloxamer for use in cell culture medium
NZ725151B2 (en) Methods of preparing a poloxamer for use in cell culture medium
HK1259741A1 (en) Serum-free cell culture medium

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40038241

Country of ref document: HK

WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20201023

WD01 Invention patent application deemed withdrawn after publication