CA2943357C - Methods of preparing a poloxamer for use in cell culture medium - Google Patents

Methods of preparing a poloxamer for use in cell culture medium Download PDF

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Publication number
CA2943357C
CA2943357C CA2943357A CA2943357A CA2943357C CA 2943357 C CA2943357 C CA 2943357C CA 2943357 A CA2943357 A CA 2943357A CA 2943357 A CA2943357 A CA 2943357A CA 2943357 C CA2943357 C CA 2943357C
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CA
Canada
Prior art keywords
poloxamer
minutes
cell
cell culture
treated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2943357A
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English (en)
French (fr)
Other versions
CA2943357A1 (en
Inventor
Ganesh Prasadh Vissvesvaran
Robert David Kiss
Steven J. Meier
Inchan Kwon
Kara Calhoun
Kate WINCHESTER
Amelia ADAMS
Marion GLENN
Stefan Koenig
Alan Deese
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Genentech Inc
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Genentech Inc
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Publication of CA2943357A1 publication Critical patent/CA2943357A1/en
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/34—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from hydroxy compounds or their metallic derivatives
    • C08G65/48—Polymers modified by chemical after-treatment
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
    • C08G65/30—Post-polymerisation treatment, e.g. recovery, purification, drying
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/34—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from hydroxy compounds or their metallic derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2650/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G2650/28—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type
    • C08G2650/50—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type containing nitrogen, e.g. polyetheramines or Jeffamines(r)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2650/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G2650/28—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type
    • C08G2650/58—Ethylene oxide or propylene oxide copolymers, e.g. pluronics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2943357A 2014-03-25 2015-03-25 Methods of preparing a poloxamer for use in cell culture medium Active CA2943357C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461970281P 2014-03-25 2014-03-25
US61/970,281 2014-03-25
PCT/US2015/022592 WO2015148736A1 (en) 2014-03-25 2015-03-25 Methods of preparing a poloxamer for use in cell culture medium

Publications (2)

Publication Number Publication Date
CA2943357A1 CA2943357A1 (en) 2015-10-01
CA2943357C true CA2943357C (en) 2023-06-27

Family

ID=52824596

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2943357A Active CA2943357C (en) 2014-03-25 2015-03-25 Methods of preparing a poloxamer for use in cell culture medium

Country Status (19)

Country Link
US (2) US11034793B2 (https=)
EP (2) EP3778702B1 (https=)
JP (3) JP6655548B2 (https=)
KR (1) KR102352250B1 (https=)
CN (2) CN106414552B (https=)
AU (1) AU2015236008B2 (https=)
BR (1) BR112016021739B1 (https=)
CA (1) CA2943357C (https=)
ES (1) ES2778680T3 (https=)
HR (1) HRP20200372T1 (https=)
IL (2) IL247902B (https=)
MX (2) MX2016012288A (https=)
MY (1) MY178060A (https=)
PL (1) PL3122799T3 (https=)
RU (1) RU2710551C2 (https=)
SG (1) SG11201607929PA (https=)
SI (1) SI3122799T1 (https=)
WO (1) WO2015148736A1 (https=)
ZA (1) ZA201606711B (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112016021739B1 (pt) 2014-03-25 2021-11-03 Genentech, Inc Método para preparar um poloxâmero para uso em um meio de cultura de célula
EP3430072B1 (en) * 2016-03-17 2020-07-01 Merck Patent GmbH Method for purifying poloxamers
JP7135096B2 (ja) * 2017-12-21 2022-09-12 シグマ－アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー ポロキサマー組成物ならびにその製造方法およびその使用
EP3790597A4 (en) * 2018-07-12 2022-03-09 Arshintseva, Elena Valentinovna THERMAL PROCESS FOR THE STERILIZATION OF POLOXAMER WITH LIQUID PHARMACEUTICALS
CN112469814A (zh) * 2018-07-27 2021-03-09 味之素株式会社 动物细胞的悬浮培养用添加物、悬浮培养用培养基和悬浮培养方法

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Also Published As

Publication number Publication date
RU2710551C2 (ru) 2019-12-27
JP2023011566A (ja) 2023-01-24
BR112016021739A2 (pt) 2017-08-15
RU2016141568A (ru) 2018-04-28
US11912823B2 (en) 2024-02-27
KR20160138161A (ko) 2016-12-02
US11034793B2 (en) 2021-06-15
MX2021001981A (es) 2021-04-28
EP3122799B1 (en) 2020-01-15
JP2017516459A (ja) 2017-06-22
AU2015236008A1 (en) 2016-10-27
SI3122799T1 (sl) 2020-06-30
JP7472222B2 (ja) 2024-04-22
WO2015148736A8 (en) 2016-08-18
EP3778702B1 (en) 2023-06-28
CN106414552B (zh) 2020-08-25
SG11201607929PA (en) 2016-10-28
EP3122799B8 (en) 2020-03-11
PL3122799T3 (pl) 2020-06-29
RU2016141568A3 (https=) 2018-09-26
WO2015148736A1 (en) 2015-10-01
BR112016021739B1 (pt) 2021-11-03
IL279583B (en) 2022-03-01
IL247902A0 (en) 2016-11-30
JP6655548B2 (ja) 2020-02-26
MX2016012288A (es) 2017-01-23
CA2943357A1 (en) 2015-10-01
ZA201606711B (en) 2019-12-18
MY178060A (en) 2020-09-30
EP3122799A1 (en) 2017-02-01
IL279583A (en) 2021-03-01
EP3778702A1 (en) 2021-02-17
IL247902B (en) 2021-01-31
ES2778680T3 (es) 2020-08-11
KR102352250B1 (ko) 2022-01-17
CN111808276A (zh) 2020-10-23
NZ725151A (en) 2021-05-28
US20210371587A1 (en) 2021-12-02
AU2015236008B2 (en) 2018-12-20
US20170009015A1 (en) 2017-01-12
CN106414552A (zh) 2017-02-15
HRP20200372T1 (hr) 2020-06-12
JP2020103290A (ja) 2020-07-09

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