CN110914414A - 利用设计dna重组酶遗传改变基因组的方法与手段 - Google Patents

利用设计dna重组酶遗传改变基因组的方法与手段 Download PDF

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CN110914414A
CN110914414A CN201880039699.9A CN201880039699A CN110914414A CN 110914414 A CN110914414 A CN 110914414A CN 201880039699 A CN201880039699 A CN 201880039699A CN 110914414 A CN110914414 A CN 110914414A
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dna
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F·布赫霍尔茨
M·施奈德
F·兰辛
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Technische Universitaet Dresden
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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CN201880039699.9A 2017-06-14 2018-06-14 利用设计dna重组酶遗传改变基因组的方法与手段 Pending CN110914414A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17175895 2017-06-14
EP17175895.6 2017-06-14
PCT/EP2018/065881 WO2018229226A1 (en) 2017-06-14 2018-06-14 Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes

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CN110914414A true CN110914414A (zh) 2020-03-24

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US (2) US11845954B2 (https=)
EP (1) EP3638776B1 (https=)
JP (2) JP7402503B2 (https=)
CN (1) CN110914414A (https=)
CA (1) CA3066047A1 (https=)
DK (1) DK3638776T3 (https=)
ES (1) ES3055471T3 (https=)
FI (1) FI3638776T3 (https=)
PL (1) PL3638776T3 (https=)
WO (1) WO2018229226A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018229226A1 (en) 2017-06-14 2018-12-20 Technische Universität Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
GB2700048B (en) 2019-09-18 2026-03-04 Aldevron Llc synthetic dna vectors and method of use
EP3831939A1 (en) * 2019-12-06 2021-06-09 Technische Universität Dresden Fusion of site-specific recombinases for efficient and specific genome editing
MX2024005779A (es) 2021-11-15 2024-05-24 Univ Dresden Tech Recombinasas especificas de sitio para edicion eficiente y especifica del genoma.
WO2025143215A1 (ja) * 2023-12-28 2025-07-03 デンカ株式会社 多型塩基を組み込んだ標的配列を作製するための組換えベクター
US20260002204A1 (en) 2024-06-07 2026-01-01 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Method for producing spacer-specific dna recombining enzymes
US20260049306A1 (en) 2024-07-25 2026-02-19 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Modifying nucleic acid sequences having asymmetric target sites

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JP2023116580A (ja) 2023-08-22
CA3066047A1 (en) 2018-12-20
FI3638776T3 (fi) 2025-12-09
US20230235364A1 (en) 2023-07-27
JP7842465B2 (ja) 2026-04-08
DK3638776T3 (da) 2025-12-15
PL3638776T3 (pl) 2026-02-23
US11845954B2 (en) 2023-12-19
US20210147878A1 (en) 2021-05-20
EP3638776B1 (en) 2025-11-05
EP3638776A1 (en) 2020-04-22
JP2020527938A (ja) 2020-09-17
JP7402503B2 (ja) 2023-12-21
WO2018229226A1 (en) 2018-12-20
ES3055471T3 (en) 2026-02-12

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