JP7402503B2 - デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 - Google Patents

デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 Download PDF

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JP7402503B2
JP7402503B2 JP2019568653A JP2019568653A JP7402503B2 JP 7402503 B2 JP7402503 B2 JP 7402503B2 JP 2019568653 A JP2019568653 A JP 2019568653A JP 2019568653 A JP2019568653 A JP 2019568653A JP 7402503 B2 JP7402503 B2 JP 7402503B2
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ブチホルツ フランク
シュナイダー マーティン
ランシング フェリックス
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テクニスチェ ユニベルシタト ドレスデン
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    • C12Q1/6869Methods for sequencing

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JP2019568653A 2017-06-14 2018-06-14 デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 Active JP7402503B2 (ja)

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018229226A1 (en) 2017-06-14 2018-12-20 Technische Universität Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
GB2700048B (en) 2019-09-18 2026-03-04 Aldevron Llc synthetic dna vectors and method of use
EP3831939A1 (en) * 2019-12-06 2021-06-09 Technische Universität Dresden Fusion of site-specific recombinases for efficient and specific genome editing
MX2024005779A (es) 2021-11-15 2024-05-24 Univ Dresden Tech Recombinasas especificas de sitio para edicion eficiente y especifica del genoma.
WO2025143215A1 (ja) * 2023-12-28 2025-07-03 デンカ株式会社 多型塩基を組み込んだ標的配列を作製するための組換えベクター
US20260002204A1 (en) 2024-06-07 2026-01-01 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Method for producing spacer-specific dna recombining enzymes
US20260049306A1 (en) 2024-07-25 2026-02-19 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Modifying nucleic acid sequences having asymmetric target sites

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170058297A1 (en) 2015-08-25 2017-03-02 Washington University Compositions and methods for site specific recombination at asymmetric sites

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4861719A (en) 1986-04-25 1989-08-29 Fred Hutchinson Cancer Research Center DNA constructs for retrovirus packaging cell lines
US5278056A (en) 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5670488A (en) 1992-12-03 1997-09-23 Genzyme Corporation Adenovirus vector for gene therapy
DE69434860T2 (de) 1993-02-22 2007-03-15 The Rockefeller University Herstellung von helfer-freien retroviren mit hohem titer mittels transienter transfektion
FR2712812B1 (fr) 1993-11-23 1996-02-09 Centre Nat Rech Scient Composition pour la production de produits thérapeutiques in vivo.
IL116816A (en) 1995-01-20 2003-05-29 Rhone Poulenc Rorer Sa Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US5981830A (en) 1997-05-30 1999-11-09 Schering Aktiengesellschaft Knockout mice and their progeny with a disrupted hepsin gene
US6774279B2 (en) 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
CA2306188C (en) 1997-11-18 2008-08-05 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign dna into eukaryotic genomes
US6187994B1 (en) 1997-11-18 2001-02-13 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
US5929301A (en) 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
DE19941186A1 (de) 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
GB0029375D0 (en) 2000-12-01 2001-01-17 Total Fina Elf Substrate linked directed evolution (slide)
DE10140030C1 (de) 2001-08-16 2002-12-19 Gsf Forschungszentrum Umwelt Einsatz mutierter Erkennungssequenzen für mehrfache aufeinander folgende Rekombinase-vermittelte Rekombinationen in einem genetischen System
WO2005081632A2 (en) 2004-02-26 2005-09-09 State Of Israel, Ministry Of Agriculture, Agricultural Research Organization Enzymes, cells and methods for site specific recombination at asymmetric sites
US7422889B2 (en) 2004-10-29 2008-09-09 Stowers Institute For Medical Research Dre recombinase and recombinase systems employing Dre recombinase
EP1942192A1 (en) 2007-01-08 2008-07-09 Heinrich-Pette-Institut für experimentelle Virologie und Immunologie Use of a tailored recombinase for the treatment of retroviral infections
DE102007001370A1 (de) 2007-01-09 2008-07-10 Curevac Gmbh RNA-kodierte Antikörper
US20100285464A1 (en) 2007-07-11 2010-11-11 Recogene Ltd A conserved region of the hiv-1 genome and uses thereof
WO2009146179A1 (en) 2008-04-15 2009-12-03 University Of Iowa Research Foundation Zinc finger nuclease for the cftr gene and methods of use thereof
US8645115B2 (en) 2008-12-22 2014-02-04 Trustees Of Boston University Modular nucleic acid-based circuits for counters, binary operations, memory and logic
DE102008063216A1 (de) 2008-12-29 2010-07-01 Wilhelm Karmann Gmbh Verdeck eines Cabriolet-Fahrzeugs mit wenigstens zwei Dachsegmenten und einem Innenhimmel
WO2010143606A1 (ja) 2009-06-08 2010-12-16 財団法人かずさディー・エヌ・エー研究所 新規な部位特異的組換え酵素とその認識配列を用いた部位特異的組換え方法
CN103154256A (zh) 2010-05-27 2013-06-12 海因里希·佩特研究所莱比锡试验病毒学研究所-民法基金会 用于使多种逆转录病毒毒株中的不对称靶位点重组的修整重组酶
RS63817B1 (sr) 2010-07-06 2023-01-31 Glaxosmithkline Biologicals Sa Čestice za isporuku slične virionu za molekule samoreplicirajuće rnk
EP3202903B1 (en) 2010-12-22 2020-02-12 President and Fellows of Harvard College Continuous directed evolution
EP2690177B1 (en) 2012-07-24 2014-12-03 Technische Universität Dresden Protein with recombinase activity for site-specific DNA-recombination
EP4293125A3 (en) 2012-12-10 2024-02-28 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2014134412A1 (en) 2013-03-01 2014-09-04 Regents Of The University Of Minnesota Talen-based gene correction
GB201414130D0 (en) 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase
EP2993229A1 (en) * 2014-09-02 2016-03-09 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
WO2016089866A1 (en) * 2014-12-01 2016-06-09 President And Fellows Of Harvard College Rna-guided systems for in vivo gene editing
CN106191041B (zh) 2015-04-30 2020-12-29 杭州菁因康生物科技有限公司 基因打靶方法
WO2017015545A1 (en) 2015-07-22 2017-01-26 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018229226A1 (en) 2017-06-14 2018-12-20 Technische Universität Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
US20220267802A1 (en) 2019-07-15 2022-08-25 President And Fellows Of Harvard College Methods and compositions for gene delivery
EP3831939A1 (en) 2019-12-06 2021-06-09 Technische Universität Dresden Fusion of site-specific recombinases for efficient and specific genome editing
WO2021158651A1 (en) 2020-02-04 2021-08-12 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for controlling production of polypeptides in cells
EP4133086A4 (en) 2020-04-07 2024-06-05 IO Biosciences, Inc. NUCLEIC ACID CONSTRUCTS WITH GENE-EDITING MULTI-SITES
GB202005180D0 (en) 2020-04-08 2020-05-20 Ge Healthcare Bio Sciences Ab Methods for targeted integration

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170058297A1 (en) 2015-08-25 2017-03-02 Washington University Compositions and methods for site specific recombination at asymmetric sites

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
J. Mol. Biol.,2008年05月02日,Vol. 378, No. 3,pp. 653-665
Nature Biotechnology,2016年04月,Vol. 34, No. 4,pp. 401-409
Nucleic Acids Research,2006年09月20日,Vol. 34, No. 18,pp. 5259-5269

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JP2023116580A (ja) 2023-08-22
CA3066047A1 (en) 2018-12-20
FI3638776T3 (fi) 2025-12-09
US20230235364A1 (en) 2023-07-27
JP7842465B2 (ja) 2026-04-08
DK3638776T3 (da) 2025-12-15
PL3638776T3 (pl) 2026-02-23
US11845954B2 (en) 2023-12-19
US20210147878A1 (en) 2021-05-20
EP3638776B1 (en) 2025-11-05
EP3638776A1 (en) 2020-04-22
JP2020527938A (ja) 2020-09-17
WO2018229226A1 (en) 2018-12-20
CN110914414A (zh) 2020-03-24
ES3055471T3 (en) 2026-02-12

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