ES3055471T3 - Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes - Google Patents
Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymesInfo
- Publication number
- ES3055471T3 ES3055471T3 ES18731446T ES18731446T ES3055471T3 ES 3055471 T3 ES3055471 T3 ES 3055471T3 ES 18731446 T ES18731446 T ES 18731446T ES 18731446 T ES18731446 T ES 18731446T ES 3055471 T3 ES3055471 T3 ES 3055471T3
- Authority
- ES
- Spain
- Prior art keywords
- sequence
- site
- dna recombination
- target
- sequences
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Ecology (AREA)
- Mycology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17175895 | 2017-06-14 | ||
| PCT/EP2018/065881 WO2018229226A1 (en) | 2017-06-14 | 2018-06-14 | Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES3055471T3 true ES3055471T3 (en) | 2026-02-12 |
Family
ID=59269741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES18731446T Active ES3055471T3 (en) | 2017-06-14 | 2018-06-14 | Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11845954B2 (https=) |
| EP (1) | EP3638776B1 (https=) |
| JP (2) | JP7402503B2 (https=) |
| CN (1) | CN110914414A (https=) |
| CA (1) | CA3066047A1 (https=) |
| DK (1) | DK3638776T3 (https=) |
| ES (1) | ES3055471T3 (https=) |
| FI (1) | FI3638776T3 (https=) |
| PL (1) | PL3638776T3 (https=) |
| WO (1) | WO2018229226A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018229226A1 (en) | 2017-06-14 | 2018-12-20 | Technische Universität Dresden | Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes |
| GB2700048B (en) | 2019-09-18 | 2026-03-04 | Aldevron Llc | synthetic dna vectors and method of use |
| EP3831939A1 (en) * | 2019-12-06 | 2021-06-09 | Technische Universität Dresden | Fusion of site-specific recombinases for efficient and specific genome editing |
| MX2024005779A (es) | 2021-11-15 | 2024-05-24 | Univ Dresden Tech | Recombinasas especificas de sitio para edicion eficiente y especifica del genoma. |
| WO2025143215A1 (ja) * | 2023-12-28 | 2025-07-03 | デンカ株式会社 | 多型塩基を組み込んだ標的配列を作製するための組換えベクター |
| US20260002204A1 (en) | 2024-06-07 | 2026-01-01 | Technische Universität Dresden Körperschaft Des Öffentlichen Rechts | Method for producing spacer-specific dna recombining enzymes |
| US20260049306A1 (en) | 2024-07-25 | 2026-02-19 | Technische Universität Dresden Körperschaft Des Öffentlichen Rechts | Modifying nucleic acid sequences having asymmetric target sites |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1293460C (en) | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
| US4861719A (en) | 1986-04-25 | 1989-08-29 | Fred Hutchinson Cancer Research Center | DNA constructs for retrovirus packaging cell lines |
| US5278056A (en) | 1988-02-05 | 1994-01-11 | The Trustees Of Columbia University In The City Of New York | Retroviral packaging cell lines and process of using same |
| US5670488A (en) | 1992-12-03 | 1997-09-23 | Genzyme Corporation | Adenovirus vector for gene therapy |
| DE69434860T2 (de) | 1993-02-22 | 2007-03-15 | The Rockefeller University | Herstellung von helfer-freien retroviren mit hohem titer mittels transienter transfektion |
| FR2712812B1 (fr) | 1993-11-23 | 1996-02-09 | Centre Nat Rech Scient | Composition pour la production de produits thérapeutiques in vivo. |
| IL116816A (en) | 1995-01-20 | 2003-05-29 | Rhone Poulenc Rorer Sa | Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof |
| US6013516A (en) | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
| US5981830A (en) | 1997-05-30 | 1999-11-09 | Schering Aktiengesellschaft | Knockout mice and their progeny with a disrupted hepsin gene |
| US6774279B2 (en) | 1997-05-30 | 2004-08-10 | Carnegie Institution Of Washington | Use of FLP recombinase in mice |
| CA2306188C (en) | 1997-11-18 | 2008-08-05 | Pioneer Hi-Bred International, Inc. | A novel method for the integration of foreign dna into eukaryotic genomes |
| US6187994B1 (en) | 1997-11-18 | 2001-02-13 | Pioneer Hi-Bred International, Inc. | Compositions and methods for genetic modification of plants |
| US5929301A (en) | 1997-11-18 | 1999-07-27 | Pioneer Hi-Bred International | Nucleic acid sequence encoding FLP recombinase |
| DE19941186A1 (de) | 1999-08-30 | 2001-03-01 | Peter Droege | Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen |
| GB0029375D0 (en) | 2000-12-01 | 2001-01-17 | Total Fina Elf | Substrate linked directed evolution (slide) |
| DE10140030C1 (de) | 2001-08-16 | 2002-12-19 | Gsf Forschungszentrum Umwelt | Einsatz mutierter Erkennungssequenzen für mehrfache aufeinander folgende Rekombinase-vermittelte Rekombinationen in einem genetischen System |
| WO2005081632A2 (en) | 2004-02-26 | 2005-09-09 | State Of Israel, Ministry Of Agriculture, Agricultural Research Organization | Enzymes, cells and methods for site specific recombination at asymmetric sites |
| US7422889B2 (en) | 2004-10-29 | 2008-09-09 | Stowers Institute For Medical Research | Dre recombinase and recombinase systems employing Dre recombinase |
| EP1942192A1 (en) | 2007-01-08 | 2008-07-09 | Heinrich-Pette-Institut für experimentelle Virologie und Immunologie | Use of a tailored recombinase for the treatment of retroviral infections |
| DE102007001370A1 (de) | 2007-01-09 | 2008-07-10 | Curevac Gmbh | RNA-kodierte Antikörper |
| US20100285464A1 (en) | 2007-07-11 | 2010-11-11 | Recogene Ltd | A conserved region of the hiv-1 genome and uses thereof |
| WO2009146179A1 (en) | 2008-04-15 | 2009-12-03 | University Of Iowa Research Foundation | Zinc finger nuclease for the cftr gene and methods of use thereof |
| US8645115B2 (en) | 2008-12-22 | 2014-02-04 | Trustees Of Boston University | Modular nucleic acid-based circuits for counters, binary operations, memory and logic |
| DE102008063216A1 (de) | 2008-12-29 | 2010-07-01 | Wilhelm Karmann Gmbh | Verdeck eines Cabriolet-Fahrzeugs mit wenigstens zwei Dachsegmenten und einem Innenhimmel |
| WO2010143606A1 (ja) | 2009-06-08 | 2010-12-16 | 財団法人かずさディー・エヌ・エー研究所 | 新規な部位特異的組換え酵素とその認識配列を用いた部位特異的組換え方法 |
| CN103154256A (zh) | 2010-05-27 | 2013-06-12 | 海因里希·佩特研究所莱比锡试验病毒学研究所－民法基金会 | 用于使多种逆转录病毒毒株中的不对称靶位点重组的修整重组酶 |
| RS63817B1 (sr) | 2010-07-06 | 2023-01-31 | Glaxosmithkline Biologicals Sa | Čestice za isporuku slične virionu za molekule samoreplicirajuće rnk |
| EP3202903B1 (en) | 2010-12-22 | 2020-02-12 | President and Fellows of Harvard College | Continuous directed evolution |
| EP2690177B1 (en) | 2012-07-24 | 2014-12-03 | Technische Universität Dresden | Protein with recombinase activity for site-specific DNA-recombination |
| EP4293125A3 (en) | 2012-12-10 | 2024-02-28 | Resolution Bioscience, Inc. | Methods for targeted genomic analysis |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| WO2014134412A1 (en) | 2013-03-01 | 2014-09-04 | Regents Of The University Of Minnesota | Talen-based gene correction |
| GB201414130D0 (en) | 2014-08-08 | 2014-09-24 | Agency Science Tech & Res | Mutants of the bacteriophage lambda integrase |
| EP2993229A1 (en) * | 2014-09-02 | 2016-03-09 | Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - | Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains |
| WO2016089866A1 (en) * | 2014-12-01 | 2016-06-09 | President And Fellows Of Harvard College | Rna-guided systems for in vivo gene editing |
| CN106191041B (zh) | 2015-04-30 | 2020-12-29 | 杭州菁因康生物科技有限公司 | 基因打靶方法 |
| WO2017015545A1 (en) | 2015-07-22 | 2017-01-26 | President And Fellows Of Harvard College | Evolution of site-specific recombinases |
| US10017832B2 (en) * | 2015-08-25 | 2018-07-10 | Washington University | Compositions and methods for site specific recombination at asymmetric sites |
| US11078493B2 (en) | 2016-06-21 | 2021-08-03 | Nanyang Technological University | Site-specific DNA recombination |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018229226A1 (en) | 2017-06-14 | 2018-12-20 | Technische Universität Dresden | Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes |
| US20220267802A1 (en) | 2019-07-15 | 2022-08-25 | President And Fellows Of Harvard College | Methods and compositions for gene delivery |
| EP3831939A1 (en) | 2019-12-06 | 2021-06-09 | Technische Universität Dresden | Fusion of site-specific recombinases for efficient and specific genome editing |
| WO2021158651A1 (en) | 2020-02-04 | 2021-08-12 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods for controlling production of polypeptides in cells |
| EP4133086A4 (en) | 2020-04-07 | 2024-06-05 | IO Biosciences, Inc. | NUCLEIC ACID CONSTRUCTS WITH GENE-EDITING MULTI-SITES |
| GB202005180D0 (en) | 2020-04-08 | 2020-05-20 | Ge Healthcare Bio Sciences Ab | Methods for targeted integration |
-
2018
- 2018-06-14 WO PCT/EP2018/065881 patent/WO2018229226A1/en not_active Ceased
- 2018-06-14 CA CA3066047A patent/CA3066047A1/en active Pending
- 2018-06-14 PL PL18731446.3T patent/PL3638776T3/pl unknown
- 2018-06-14 US US16/622,937 patent/US11845954B2/en active Active
- 2018-06-14 EP EP18731446.3A patent/EP3638776B1/en active Active
- 2018-06-14 CN CN201880039699.9A patent/CN110914414A/zh active Pending
- 2018-06-14 ES ES18731446T patent/ES3055471T3/es active Active
- 2018-06-14 DK DK18731446.3T patent/DK3638776T3/da active
- 2018-06-14 FI FIEP18731446.3T patent/FI3638776T3/fi active
- 2018-06-14 JP JP2019568653A patent/JP7402503B2/ja active Active
-
2022
- 2022-10-12 US US18/046,066 patent/US20230235364A1/en active Pending
-
2023
- 2023-06-05 JP JP2023092153A patent/JP7842465B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2023116580A (ja) | 2023-08-22 |
| CA3066047A1 (en) | 2018-12-20 |
| FI3638776T3 (fi) | 2025-12-09 |
| US20230235364A1 (en) | 2023-07-27 |
| JP7842465B2 (ja) | 2026-04-08 |
| DK3638776T3 (da) | 2025-12-15 |
| PL3638776T3 (pl) | 2026-02-23 |
| US11845954B2 (en) | 2023-12-19 |
| US20210147878A1 (en) | 2021-05-20 |
| EP3638776B1 (en) | 2025-11-05 |
| EP3638776A1 (en) | 2020-04-22 |
| JP2020527938A (ja) | 2020-09-17 |
| JP7402503B2 (ja) | 2023-12-21 |
| WO2018229226A1 (en) | 2018-12-20 |
| CN110914414A (zh) | 2020-03-24 |
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