CN110691852A - Alk、ret和ros融合物的多重pcr检测 - Google Patents

Alk、ret和ros融合物的多重pcr检测 Download PDF

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Publication number
CN110691852A
CN110691852A CN201880035309.0A CN201880035309A CN110691852A CN 110691852 A CN110691852 A CN 110691852A CN 201880035309 A CN201880035309 A CN 201880035309A CN 110691852 A CN110691852 A CN 110691852A
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exon
alk
ros1
ret
fusion gene
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Chinese (zh)
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A.贝戈维奇
C.张
J.池
G.希尔曼
D.郭
M.李
X.M.马
C.马诺哈尔
E.奥尔迪纳里奥
J.拉贾马尼
H.张
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F Hoffmann La Roche AG
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Publication of CN110691852A publication Critical patent/CN110691852A/zh
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CN201880035309.0A 2017-05-31 2018-05-30 Alk、ret和ros融合物的多重pcr检测 Pending CN110691852A (zh)

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US201762513226P 2017-05-31 2017-05-31
US62/513226 2017-05-31
PCT/EP2018/064172 WO2018220004A1 (en) 2017-05-31 2018-05-30 Multiplex pcr detection of alk, ret, and ros fusions

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US (2) US11505830B2 (enExample)
EP (1) EP3631011B1 (enExample)
JP (2) JP2020521440A (enExample)
CN (1) CN110691852A (enExample)
AU (1) AU2018277210B2 (enExample)
CA (1) CA3059119C (enExample)
ES (1) ES2883934T3 (enExample)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112029857A (zh) * 2020-08-14 2020-12-04 上海真固生物科技有限公司 一种引物组合物及用于融合基因检测的试剂
CN115466788A (zh) * 2022-06-28 2022-12-13 湖南圣洲生物科技有限公司 引物探针组合、试剂盒及其检测人alk、ros1基因融合的方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3631011B1 (en) 2017-05-31 2021-06-23 Roche Diagnostics GmbH Multiplex pcr detection of alk, ret, and ros fusions
CN109837275B (zh) * 2019-01-08 2022-09-06 大连医科大学附属第二医院 一种融合基因阳性对照标准品的制备方法
CN111718994A (zh) * 2019-03-22 2020-09-29 广州金域医学检验中心有限公司 肿瘤融合基因靶向检测探针、试剂盒及应用
CN110964830A (zh) * 2019-12-31 2020-04-07 中山大学达安基因股份有限公司 多重检测ros1基因突变的试剂盒及方法
CN117144016A (zh) * 2023-10-27 2023-12-01 神州医疗科技股份有限公司 一种用于外周血循环肿瘤rna中检测ret融合基因的引物探针、试剂盒及检测系统

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101466721A (zh) * 2007-04-13 2009-06-24 细胞信号技术公司 在人实体瘤中的基因缺损和突变体alk激酶
CN104818320A (zh) * 2014-12-03 2015-08-05 厦门艾德生物医药科技有限公司 一次性检测肺癌多重基因的引物、探针、检测体系和试剂盒
CN105102635A (zh) * 2013-03-15 2015-11-25 生命技术公司 肺癌的分类和可行性指数

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
AU762754B2 (en) * 1999-02-18 2003-07-03 Regents Of The University Of California, The Salicylamide-lanthanide complexes for use as luminescent markers
CA2405687A1 (en) * 2000-04-05 2001-10-18 Molecular Staging, Inc. Process for allele discrimation utilizing primer extension
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
PL2450437T3 (pl) * 2006-04-14 2017-12-29 Cell Signaling Technology Inc Defekty genu i zmutowana kinaza ALK w ludzkich guzach litych
EP2775300A3 (en) * 2009-08-28 2015-04-01 Asuragen, INC. miRNA Biomarkers of Lung Disease
JP5861244B2 (ja) 2010-06-22 2016-02-16 公益財団法人がん研究会 新規ｒｏｓ１融合体の検出法
JPWO2012014795A1 (ja) * 2010-07-26 2013-09-12 アステラス製薬株式会社 新規ｒｅｔ融合体の検出法
US20130059762A1 (en) * 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US20130237442A1 (en) * 2012-03-07 2013-09-12 University Of Miami Methods and Compositions for Diagnosis of Non-Viable Early Pregnancy
CA2902167A1 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Method for amplification and assay of rna fusion gene variants, method of distinguishing same and related primers, probes, and kits
US10072298B2 (en) 2013-04-17 2018-09-11 Life Technologies Corporation Gene fusions and gene variants associated with cancer
WO2015148494A1 (en) 2014-03-25 2015-10-01 Quest Diagnostics Investments Incorporated Detection of gene fusions by intragenic differential expression (ide) using average cycle thresholds
CN106414768B (zh) 2014-03-27 2020-05-29 生命技术公司 与癌症相关的基因融合体和基因变异体
JP6696908B2 (ja) * 2014-04-25 2020-05-20 エグゼリクシス， インコーポレイテッド 肺腺癌の治療方法
JP6856544B2 (ja) * 2015-04-17 2021-04-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 遺伝子融合体を検出するための多重ｐｃｒ
EP3631011B1 (en) 2017-05-31 2021-06-23 Roche Diagnostics GmbH Multiplex pcr detection of alk, ret, and ros fusions

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101466721A (zh) * 2007-04-13 2009-06-24 细胞信号技术公司 在人实体瘤中的基因缺损和突变体alk激酶
CN105102635A (zh) * 2013-03-15 2015-11-25 生命技术公司 肺癌的分类和可行性指数
CN104818320A (zh) * 2014-12-03 2015-08-05 厦门艾德生物医药科技有限公司 一次性检测肺癌多重基因的引物、探针、检测体系和试剂盒

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MARUJA E LIRA等: "A single-tube multiplexed assay for detecting ALK, ROS1, and RET fusions in lung cancer" *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112029857A (zh) * 2020-08-14 2020-12-04 上海真固生物科技有限公司 一种引物组合物及用于融合基因检测的试剂
CN112029857B (zh) * 2020-08-14 2026-03-24 上海真固生物科技有限公司 一种引物组合物及用于融合基因检测的试剂
CN115466788A (zh) * 2022-06-28 2022-12-13 湖南圣洲生物科技有限公司 引物探针组合、试剂盒及其检测人alk、ros1基因融合的方法
CN115466788B (zh) * 2022-06-28 2026-02-10 湖南圣洲生物科技有限公司 引物探针组合、试剂盒及其检测人alk、ros1基因融合的方法

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WO2018220004A1 (en) 2018-12-06
US20230121541A1 (en) 2023-04-20
AU2018277210A1 (en) 2019-10-17
CA3059119A1 (en) 2018-12-06
CA3059119C (en) 2024-01-23
AU2018277210B2 (en) 2024-03-28
ES2883934T3 (es) 2021-12-09
US20180346994A1 (en) 2018-12-06
US11505830B2 (en) 2022-11-22
EP3631011A1 (en) 2020-04-08
JP2024019433A (ja) 2024-02-09
JP2020521440A (ja) 2020-07-27
EP3631011B1 (en) 2021-06-23

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