CA3059119C - Multiplex pcr detection of alk, ret, and ros fusions - Google Patents

Multiplex pcr detection of alk, ret, and ros fusions Download PDF

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Publication number
CA3059119C
CA3059119C CA3059119A CA3059119A CA3059119C CA 3059119 C CA3059119 C CA 3059119C CA 3059119 A CA3059119 A CA 3059119A CA 3059119 A CA3059119 A CA 3059119A CA 3059119 C CA3059119 C CA 3059119C
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exon
alk
ros1
ret
fusion gene
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French (fr)
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CA3059119A1 (en
Inventor
Ann Begovich
Cindy Cheung
Javelin CHI
Grantland HILLMAN
Dwight KUO
Michael Lee
Xiaoju Max Ma
Chitra MANOHAR
Ellen ORDINARIO
Jaya RAJAMANI
Huan Truong
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3059119A 2017-05-31 2018-05-30 Multiplex pcr detection of alk, ret, and ros fusions Active CA3059119C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762513226P 2017-05-31 2017-05-31
US62/513,226 2017-05-31
PCT/EP2018/064172 WO2018220004A1 (en) 2017-05-31 2018-05-30 Multiplex pcr detection of alk, ret, and ros fusions

Publications (2)

Publication Number Publication Date
CA3059119A1 CA3059119A1 (en) 2018-12-06
CA3059119C true CA3059119C (en) 2024-01-23

Family

ID=62567625

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CA3059119A Active CA3059119C (en) 2017-05-31 2018-05-30 Multiplex pcr detection of alk, ret, and ros fusions

Country Status (8)

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US (2) US11505830B2 (enExample)
EP (1) EP3631011B1 (enExample)
JP (2) JP2020521440A (enExample)
CN (1) CN110691852A (enExample)
AU (1) AU2018277210B2 (enExample)
CA (1) CA3059119C (enExample)
ES (1) ES2883934T3 (enExample)
WO (1) WO2018220004A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3631011B1 (en) 2017-05-31 2021-06-23 Roche Diagnostics GmbH Multiplex pcr detection of alk, ret, and ros fusions
CN109837275B (zh) * 2019-01-08 2022-09-06 大连医科大学附属第二医院 一种融合基因阳性对照标准品的制备方法
CN111718994A (zh) * 2019-03-22 2020-09-29 广州金域医学检验中心有限公司 肿瘤融合基因靶向检测探针、试剂盒及应用
CN110964830A (zh) * 2019-12-31 2020-04-07 中山大学达安基因股份有限公司 多重检测ros1基因突变的试剂盒及方法
CN112029857B (zh) * 2020-08-14 2026-03-24 上海真固生物科技有限公司 一种引物组合物及用于融合基因检测的试剂
CN115466788B (zh) * 2022-06-28 2026-02-10 湖南圣洲生物科技有限公司 引物探针组合、试剂盒及其检测人alk、ros1基因融合的方法
CN117144016A (zh) * 2023-10-27 2023-12-01 神州医疗科技股份有限公司 一种用于外周血循环肿瘤rna中检测ret融合基因的引物探针、试剂盒及检测系统

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US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
AU762754B2 (en) * 1999-02-18 2003-07-03 Regents Of The University Of California, The Salicylamide-lanthanide complexes for use as luminescent markers
CA2405687A1 (en) * 2000-04-05 2001-10-18 Molecular Staging, Inc. Process for allele discrimation utilizing primer extension
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
PL2450437T3 (pl) * 2006-04-14 2017-12-29 Cell Signaling Technology Inc Defekty genu i zmutowana kinaza ALK w ludzkich guzach litych
WO2008127248A1 (en) * 2007-04-13 2008-10-23 Cell Signaling Technology, Inc. Gene defects and mutant alk kinase in human solid tumors
EP2775300A3 (en) * 2009-08-28 2015-04-01 Asuragen, INC. miRNA Biomarkers of Lung Disease
JP5861244B2 (ja) 2010-06-22 2016-02-16 公益財団法人がん研究会 新規ｒｏｓ１融合体の検出法
JPWO2012014795A1 (ja) * 2010-07-26 2013-09-12 アステラス製薬株式会社 新規ｒｅｔ融合体の検出法
US20130059762A1 (en) * 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US20130237442A1 (en) * 2012-03-07 2013-09-12 University Of Miami Methods and Compositions for Diagnosis of Non-Viable Early Pregnancy
CN105102635B (zh) * 2013-03-15 2020-09-25 生命技术公司 肺癌的分类和可行性指数
CA2902167A1 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Method for amplification and assay of rna fusion gene variants, method of distinguishing same and related primers, probes, and kits
US10072298B2 (en) 2013-04-17 2018-09-11 Life Technologies Corporation Gene fusions and gene variants associated with cancer
WO2015148494A1 (en) 2014-03-25 2015-10-01 Quest Diagnostics Investments Incorporated Detection of gene fusions by intragenic differential expression (ide) using average cycle thresholds
CN106414768B (zh) 2014-03-27 2020-05-29 生命技术公司 与癌症相关的基因融合体和基因变异体
JP6696908B2 (ja) * 2014-04-25 2020-05-20 エグゼリクシス， インコーポレイテッド 肺腺癌の治療方法
CN104818320B (zh) * 2014-12-03 2018-02-06 厦门艾德生物医药科技有限公司 一次性检测肺癌多重基因的引物、探针、检测体系和试剂盒
JP6856544B2 (ja) * 2015-04-17 2021-04-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 遺伝子融合体を検出するための多重ｐｃｒ
EP3631011B1 (en) 2017-05-31 2021-06-23 Roche Diagnostics GmbH Multiplex pcr detection of alk, ret, and ros fusions

Also Published As

Publication number Publication date
WO2018220004A1 (en) 2018-12-06
US20230121541A1 (en) 2023-04-20
CN110691852A (zh) 2020-01-14
AU2018277210A1 (en) 2019-10-17
CA3059119A1 (en) 2018-12-06
AU2018277210B2 (en) 2024-03-28
ES2883934T3 (es) 2021-12-09
US20180346994A1 (en) 2018-12-06
US11505830B2 (en) 2022-11-22
EP3631011A1 (en) 2020-04-08
JP2024019433A (ja) 2024-02-09
JP2020521440A (ja) 2020-07-27
EP3631011B1 (en) 2021-06-23

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