AU2018277210B2 - Multiplex PCR detection of ALK, RET, and ROS fusions - Google Patents

Multiplex PCR detection of ALK, RET, and ROS fusions Download PDF

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Publication number
AU2018277210B2
AU2018277210B2 AU2018277210A AU2018277210A AU2018277210B2 AU 2018277210 B2 AU2018277210 B2 AU 2018277210B2 AU 2018277210 A AU2018277210 A AU 2018277210A AU 2018277210 A AU2018277210 A AU 2018277210A AU 2018277210 B2 AU2018277210 B2 AU 2018277210B2
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exon
alk
ret
fusion gene
fusion
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AU2018277210A1 (en
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Ann Begovich
Cindy Cheung
Javelin CHI
Grantland HILLMAN
Dwight KUO
Michael Lee
Xiaoju Max Ma
Chitra MANOHAR
Ellen ORDINARIO
Jaya RAJAMANI
Huan Truong
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2600/00—Oligonucleotides characterized by their use
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2018277210A 2017-05-31 2018-05-30 Multiplex PCR detection of ALK, RET, and ROS fusions Active AU2018277210B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762513226P 2017-05-31 2017-05-31
US62/513,226 2017-05-31
PCT/EP2018/064172 WO2018220004A1 (en) 2017-05-31 2018-05-30 Multiplex pcr detection of alk, ret, and ros fusions

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AU2018277210A1 AU2018277210A1 (en) 2019-10-17
AU2018277210B2 true AU2018277210B2 (en) 2024-03-28

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US (2) US11505830B2 (enExample)
EP (1) EP3631011B1 (enExample)
JP (2) JP2020521440A (enExample)
CN (1) CN110691852A (enExample)
AU (1) AU2018277210B2 (enExample)
CA (1) CA3059119C (enExample)
ES (1) ES2883934T3 (enExample)
WO (1) WO2018220004A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3059119C (en) 2017-05-31 2024-01-23 F. Hoffman-La Roche Ag Multiplex pcr detection of alk, ret, and ros fusions
CN109837275B (zh) * 2019-01-08 2022-09-06 大连医科大学附属第二医院 一种融合基因阳性对照标准品的制备方法
CN111718994A (zh) * 2019-03-22 2020-09-29 广州金域医学检验中心有限公司 肿瘤融合基因靶向检测探针、试剂盒及应用
CN110964830A (zh) * 2019-12-31 2020-04-07 中山大学达安基因股份有限公司 多重检测ros1基因突变的试剂盒及方法
CN112029857A (zh) * 2020-08-14 2020-12-04 上海真固生物科技有限公司 一种引物组合物及用于融合基因检测的试剂
CN115466788A (zh) * 2022-06-28 2022-12-13 湖南圣洲生物科技有限公司 引物探针组合、试剂盒及其检测人alk、ros1基因融合的方法
CN117144016A (zh) * 2023-10-27 2023-12-01 神州医疗科技股份有限公司 一种用于外周血循环肿瘤rna中检测ret融合基因的引物探针、试剂盒及检测系统

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US20150315657A1 (en) * 2013-04-17 2015-11-05 Life Technologies Corporation Gene fusions and gene variants associated with cancer
WO2016166269A1 (en) * 2015-04-17 2016-10-20 Roche Diagnostics Gmbh Multiplex pcr to detect gene fusions

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JP4943585B2 (ja) * 1999-02-18 2012-05-30 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 発光マーカーとして用いるためのサリチルアミド−ランタニド錯体
CA2405687A1 (en) * 2000-04-05 2001-10-18 Molecular Staging, Inc. Process for allele discrimation utilizing primer extension
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
ES2561406T3 (es) * 2006-04-14 2016-02-26 Cell Signaling Technology, Inc. Defectos de genes y quinasa ALK mutante en tumores sólidos humanos
JP5562640B2 (ja) * 2007-04-13 2014-07-30 セル・シグナリング・テクノロジー・インコーポレイテツド ヒト固形腫瘍における遺伝子欠失及び突然変異ａｌｋキナーゼ
US8735074B2 (en) * 2009-08-28 2014-05-27 Asuragen, Inc. MiRNA biomarkers of lung disease
US9109259B2 (en) * 2010-06-22 2015-08-18 Japanese Foundation For Cancer Research Detection method for novel ROS1 fusions
EP2599878A4 (en) * 2010-07-26 2014-02-12 Astellas Pharma Inc NEW METHOD FOR DETECTING FUSED BODIES RET
US20130059762A1 (en) * 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US20130237442A1 (en) * 2012-03-07 2013-09-12 University Of Miami Methods and Compositions for Diagnosis of Non-Viable Early Pregnancy
WO2014150300A2 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Method for amplification and assay of rna fusion gene variants, method of distinguishing same and related primers, probes, and kits
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EP3825421B1 (en) 2014-03-25 2022-06-22 Quest Diagnostics Investments Incorporated Detection of gene fusions by intragenic differential expression (ide) using average cycle thresholds
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WO2016166269A1 (en) * 2015-04-17 2016-10-20 Roche Diagnostics Gmbh Multiplex pcr to detect gene fusions

Also Published As

Publication number Publication date
CA3059119A1 (en) 2018-12-06
CA3059119C (en) 2024-01-23
EP3631011B1 (en) 2021-06-23
JP2020521440A (ja) 2020-07-27
ES2883934T3 (es) 2021-12-09
CN110691852A (zh) 2020-01-14
JP2024019433A (ja) 2024-02-09
US20180346994A1 (en) 2018-12-06
US11505830B2 (en) 2022-11-22
EP3631011A1 (en) 2020-04-08
WO2018220004A1 (en) 2018-12-06
US20230121541A1 (en) 2023-04-20
AU2018277210A1 (en) 2019-10-17

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