CN111718994A - 肿瘤融合基因靶向检测探针、试剂盒及应用 - Google Patents
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Abstract
本发明涉及一种肿瘤融合基因靶向检测探针、试剂盒及应用。该探针基于DNA水平发生融合突变主要区域进行靶向捕获，均衡覆盖程度及特异性进行的优化，对融合突变高发的目标区域实现了理想的覆盖，并具有高度均一性，其不仅适用于肿瘤组织样本或穿刺组织样本，同时适用于血浆游离DNA的捕获，以给随访检测或药效检测时，无法多次获得组织/细胞样本的病患提供标本源保障。并且探针设计基于液相杂交方法对目标区域进行捕获，可以对新发的融合事件进行检测及报告，相比扩增方法更具优越性；而且设计的探针针对融合检测技术灵敏度也远高于目前传统检测技术，通过高深度测序可以检出突变频率1％及以下的融合，同时可以对具体的融合断裂位点进行报告。
Description
技术领域
本发明涉及肿瘤检测领域，尤其是涉及一种肿瘤融合基因靶向检测探针、试剂盒及应用。
背景技术
目前认为肿瘤发生是多基因突变累积导致的结果，最终表现为肿瘤的多种生物学特性异常，如失控性增殖、逃避生长抑制、抗凋亡、复制和永生化、促血管生成、侵袭和转移、能量代谢紊乱、促进炎症、免疫逃逸以及基因组不稳定等。
随着肿瘤分子生物学与基因组学的发展，人们对肿瘤分子表型的认识也不断加深，肿瘤诊治的策略从既往围绕发病部位和形态病理为核心的系统化疗阶段，逐渐转变为针对驱动基因突变的精准靶向治疗时代。基于特定基因变异给予相应的靶向治疗是当前肿瘤治疗的首选方案，不同瘤种在肿瘤发生、发展过程中存在相同的信号通路激活或基因变异，“异病同治”的思路为临床靶向药物的探索提供了参考。此外，靶向治疗不可避免会发生耐药，而导致耐药的机制非常复杂。因此，多基因平行检测是指导肿瘤靶向治疗的趋势。传统的实体肿瘤融合基因检测时内含子等复杂序列区域位点覆盖不理想，限制了肿瘤多基因检测的应用。
发明内容
基于此，有必要提供一种覆盖度高的肿瘤融合基因靶向检测探针、试剂盒及应用。
一种肿瘤融合基因靶向检测探针，包括针对如下各染色体上内含子的不同起始位点或其附近位点与终止位点或其附近位点之间的基因片段按照预设长度构建的、与所述基因片段互补配对的检测探针中的至少一条：
参考基因组是hg19。
在其中一个实施例中，所述肿瘤融合基因靶向检测探针包括针对如下各内含子的不同起始位点与终止位点之间的基因片段按照预设长度构建的、与所述基因片段互补配对的检测探针中的至少一条：
参考基因组是hg19。
在其中一个实施例中，所述预设长度是100bp-140bp，优选为120bp。
在其中一个实施例中，所述肿瘤融合基因靶向检测探针包括序列如SEQ ID NO.1-SEQ ID NO.187所示的187条检测探针。
在其中一个实施例中，所述肿瘤融合基因靶向检测探针还包括针对如下各染色体上内含子或外显子的不同起始位点或其附近位点与终止位点或其附近位点之间的基因片段按照预设长度构建的、与所述基因片段互补配对的检测探针中的至少一条：
参考基因组是hg19。
在其中一个实施例中，所述肿瘤融合基因靶向检测探针还包括序列如SEQ IDNO.188-SEQ ID NO.229所示的42条检测探针。
上述任一实施例所述的肿瘤融合基因靶向检测探针在制备用于肿瘤分型的检测试剂或检测芯片中的应用。
一种肿瘤融合基因靶向检测试剂盒，含有上述任一实施例所述的肿瘤融合基因靶向检测探针。
在其中一个实施例中，所述肿瘤融合基因靶向检测试剂盒还含有核酸提取试剂、文库构建试剂、样品及文库定量试剂、片段质量控制试剂、杂交捕获试剂及核酸纯化试剂中的至少一种试剂。
一种上述肿瘤融合基因靶向检测试剂盒的使用方法，包括如下步骤：
提取肿瘤组织细胞的DNA；
使用所述检测探针捕获目标片段；
对捕获的目标片段进行测序；
生物信息分析鉴定融合突变的序列。
本发明构建的融合基因靶向检测探针及试剂盒至少具有如下有益效果：
1.该靶向检测探针设计基于DNA水平发生融合突变主要区域进行靶向捕获，均衡覆盖程度及特异性进行的优化，对融合突变高发的目标区域实现了理想的覆盖，并具有高度均一性；
2.ROS1_Intron_31-32区域60％序列为高度重复或同源区，无法满足探针设计条件，针对在该区域高发融合事件的5'Partner Gene(CD74、SLC34A2、SDC4)设计配对探针，可以避免该区域融合事件的漏检；
3.该靶向检测探针设计不仅适用于肿瘤组织样本或穿刺组织样本，同时适用于血浆游离DNA的捕获，以给随访检测或药效检测时，无法多次获得组织/细胞样本的病患提供标本源保障；
4.该靶向检测探针设计基于液相杂交方法对目标区域进行捕获，可以对新发的融合事件进行检测及报告，相比扩增方法(只能针对已知的融合事件进行检测)更具优越性；
5.该靶向检测探针设计针对融合检测技术灵敏度也远高于目前传统检测技术，通过高深度测序可以检出突变频率1％及以下的融合，同时可以对具体的融合断裂位点进行报告。
附图说明
图1为不同染色体上用于设计靶向检测探针的目的基因片段的分布示意图；
图2为本发明设计的探针在目标区域(ALK基因)覆盖程度及均一性示意；
图3为FISH验证结果。
具体实施方式
为了便于理解本发明，下面将参照相关附图对本发明进行更全面的描述。附图中给出了本发明的较佳实施例。但是，本发明可以以许多不同的形式来实现，并不限于本文所描述的实施例。相反地，提供这些实施例的目的是使对本发明的公开内容的理解更加透彻全面。
除非另有定义，本文所使用的所有的技术和科学术语与属于本发明的技术领域的技术人员通常理解的含义相同。本文中在本发明的说明书中所使用的术语只是为了描述具体的实施例的目的，不是旨在于限制本发明。本文所使用的术语“和/或”包括一个或多个相关的所列项目的任意的和所有的组合。
本发明提供了一种肿瘤融合基因靶向检测探针，请结合图1，其包括针对下表1所示各染色体上内含子的不同起始位点或其附近位点与终止位点或其附近位点之间的基因片段按照预设长度构建的、与基因片段互补配对的检测探针中的至少一条：
表1
参考基因组是hg19。
在上述表1所示各内含子的起始位点和终止位点的基础上，为进一步提高关键内含子区覆盖，有利于DNA融合水平的检出，设计时比较了Roche Nimblegen的在线工具和IDT的在线设计工具，对内含子的覆盖进行进一步的优化，如下表2所示，根据表2所示的各内含子的起始位点和终止位点之间的基因片段按照预设长度，如100bp-140bp的长度，优选120bp的长度设计检测探针，进行全片段覆盖。
表2
| 构建区域 | 染色体 | 起始位点 | 终止位点 |
| ALK_Target1 | chr2 | 29448390 | 29449830 |
| ALK_Target2 | chr2 | 29446341 | 29448381 |
| ALK_Target3 | chr2 | 29445420 | 29446260 |
| RET_Target1 | chr10 | 43604647 | 43606687 |
| RET_Target2 | chr10 | 43606870 | 43607590 |
| RET_Target3 | chr10 | 43608408 | 43609008 |
| RET_Target4 | chr10 | 43609106 | 43609946 |
| RET_Target5 | chr10 | 43610148 | 43612068 |
| ROS1_Target1 | chr6 | 117650572 | 117658372 |
| ROS1_Target2 | chr6 | 117647534 | 117650534 |
| ROS1_Target3 | chr6 | 117645523 | 117647443 |
| ROS1_Target4 | chr6 | 117642526 | 117645526 |
| ROS1_Target5 | chr6 | 117641148 | 117642468 |
设计原则及步骤如下：
1.目标区域在基因组上的坐标确定，优选如表2所示；
2.将目标区域按1×(120bp/条)进行全覆盖；
3.采用重复序列标记软件对目标区域内的序列重复区进行标记；
4.对目标区域进行全基因组blast(比对)，并输出Blast Hits数目(比对命中数目)；
5.根据Blast Hits数目进行风险等级分类，Hits>1000(直接去除)，100<Hits<1000(High)，10<Hits<100(RISK)，Hits<10(Low)；
6.选取Hits<10(Low)的探针序列为检测探针设计组；
7.进一步地，为了内含子更好低覆盖，并平衡repeat RISK(重复风险)对捕获效率的影响，再次重新计算10<Hits<30的探针数目，数目占比小于总探针数目的10％，则选取在检测探针组内。
优选地，根据上述设计原则和步骤，最终设计的肿瘤融合基因靶向检测探针包括序列如SEQ ID NO.1-SEQ ID NO.187所示的187条检测探针中的至少一条，更优选包括所有187条检测探针，具体如下表3所示。
表3
更进一步地，针对ROS1基因，如断裂发生在ROS1_Intron_31-32，便客观存在MISS(遗漏)检出的可能性，因为该区域repeat程度较高，即便优化的手工设计也只覆盖该区40％，因此反向地，设计落在此区域的高发融合事件的Partner Gene(CD74、SLC34A2、SDC4)添加探针，以提高检出可能性，也即优选地，该肿瘤融合基因靶向检测探针还包括针对下表4中各染色体上内含子或外显子的不同起始位点或其附近位点与终止位点或其附近位点之间的基因片段按照预设长度构建的、与基因片段互补配对的检测探针中的至少一条：
表4
| 构建区域 | 染色体 | 起始位点 | 终止位点 |
| CD74_Target1 | chr5 | 149782876 | 149784242 |
| CD74_Target2 | chr5 | 149784243 | 149784330 |
| SLC34A2_Target1 | chr4 | 25665824 | 25665952 |
| SLC34A2_Target2 | chr4 | 25665953 | 25667749 |
| SDC4_Target1 | chr20 | 43964422 | 43964560 |
| SDC4_Target2 | chr20 | 43961710 | 43964421 |
更优选地，该肿瘤融合基因靶向检测探针还包括序列如SEQ ID NO.188-SEQ IDNO.229所示的42条检测探针，具体如下表5所示。
表5
上述肿瘤融合基因靶向检测探针可以应用在制备用于肿瘤分型的检测试剂或检测芯片中。
本发明还提供了一种肿瘤融合基因靶向检测试剂盒，其含有上述任一实施例的肿瘤融合基因靶向检测探针。
在一个具体的示例中，肿瘤融合基因靶向检测试剂盒还含有核酸提取试剂、文库构建试剂、样品及文库定量试剂、片段质量控制试剂、杂交捕获试剂及核酸纯化试剂中的至少一种试剂。
本发明进一步还提供了一种上述肿瘤融合基因靶向检测试剂盒的使用方法，包括如下步骤：
提取肿瘤组织细胞的DNA；
使用检测探针捕获目标片段；
对捕获的目标片段进行测序；
生物信息分析鉴定融合突变的序列
以下结合具体实施例对本发明的肿瘤融合基因靶向检测探针、试剂盒及应用作进一步详细的说明。
1.如图2所示，图2所示的为本发明设计的探针在目标区域(ALK基因)覆盖程度及均一性示意，其他目标区域的类似，在此不再示意。
2.本设计探针验证检测案例及数据：
如图3所示，标本NT19D0240新发罕见融合突变BIN1-ALK，FISH验证结果显示检测到ALK基因重排，阳性细胞数/分析细胞数＝18％。
以上所述实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。
以上所述实施例仅表达了本发明的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。
序列表
<110> 广州金域医学检验中心有限公司、广州金域医学检验集团股份有限公司
<120> 肿瘤融合基因靶向检测探针、试剂盒及应用
<160> 229
<170> SIPOSequenceListing 1.0
<210> 1
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
ttccggacac ctggccttca tacacctccc caaaggcgcc atggcccaga cccctgtgca 60
aaggagaaga caagaggaga cagagtcaaa caggccacaa taatgaggca gctgggggtc 120
<210> 2
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
ctgagcctgg gcgtcacatt tagtggacaa acacgagagg cgggggtaac atacacactc 60
aggagtaata ccctcaacat ggctttgcta tgcaatctcg ggcttccagt tcttcacctg 120
<210> 3
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
aaaactggta agaaggattc caaccaggga ctcatgacaa tggtcaaatg tttattcatt 60
caatcagtag ttattgagtc tctgatattt gccagggaca caggggtgca taagccatgg 120
<210> 4
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
cttttccccg ggggaggttc atgctccttg gggagagaca gatcactgtg ggtactggct 60
ggggagtatt tccagtgagt gacagaggaa ggcgcagttg ctctagatgc tcagaggagg 120
<210> 5
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
ggccctttgc tcccctgctc ccctggaggg aaggggagcc aggtagactt ggagagagca 60
catctgaatg aattttgagg agagccctgg ttctcctctc attgtataaa ctccataaac 120
<210> 6
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
tatgggagtt aatgtaagcc aacatacatg gccctgaatg tcaaggcttg tcctcctagg 60
agggctaggg gtgcccatag ggagggctct gccggccttt tgtggctaga ggagtctgcg 120
<210> 7
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
gtgctgtgat aacattcagc ccctacactg cacccctctc ctcccaggac ggcagcaggg 60
cgctcaccga atgagggtga tgtttttccg cggcacctcc ttcaggtcac tgatggagga 120
<210> 8
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
gctctgcagc tccatctgca tggcttgcag ctcctggtgc ttccggcggt acactgcagg 60
tgggtggtca gctgcaacat ggcctggcag cctggccctt gaagcactac acaggccact 120
<210> 9
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
tcctacagga agcctccctg gatctccata tcctcccctg agctctgaac ctttccatca 60
tacttagaaa tactaataaa atgattaaag aaggtgtgtc tttaattgaa gcatgattta 120
<210> 10
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
aagtaaatgc aaagctaaaa atcagatata tggaaaataa ttatttgtat tatatagggc 60
agagtcatgt tagtctggtt cctccaagaa gcagactgga gatgggatta gacccaatat 120
<210> 11
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
ggtctgcaga ttttattaga agaaatgccc atgagaggaa atggggaggg agccagggaa 60
ggctgggtga accagcagac tgtgttgcaa gtataacccc acgtgaacga gggagggagg 120
<210> 12
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 12
gaaggttggg tggaagcacc ctgggtgcca tggagcctaa ggaagtttca gcaaggccct 60
aaggggttct ggagccaaag tcagtcatca gaggagtccc atgactccca ggaattggcc 120
<210> 13
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
tgccttagta tttctgctgt gctcagccat tgggtagggc agcttcagtg caatcacagc 60
agtggatttg agggtgcagc tgggatcttg gtcagttgtg tttcctatag ttggagaact 120
<210> 14
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 14
gccaagccac agagttggag aagagccaca tcatgaaaag atctctgaat tggtgtctgg 60
ggatctgtgc tctaattccg cctcttgtta cttacaagct gagccatgag gaccaggtca 120
<210> 15
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 15
caggacctct ttggactgca gtttccctct ctgtaggcag ggatggtaac tcctgccctg 60
tttccctaac cactgccact ccccaccctc tagggttgtc aatgaaatga attcaccaac 120
<210> 16
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
ataaaatggt tttgaaaaat cctaaagagc tctaccaatg tgagtgacca ttatcactcc 60
tacatgtgag gatgttctgg aaggcaaact ccatggaagc cagaacaaaa ttgtgattca 120
<210> 17
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
gtgggtagat tctgtgtgta aagcccagcc ccccaacaca tgggccaggg caaatgagtc 60
acccgctatg tgctcagttc cctcctctat gcaatggacc gaccgtgatc agattagggt 120
<210> 18
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
tacctgagga tcgaatgaat tgaaatgtgt aaattgccga gcacgtagta accatgcaac 60
aagtgttagc tcctattatc ctgtcccttt gagggatggc accatatggg gacacagtgt 120
<210> 19
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
gtgctgccat ctcccttcta ccggcagatc cctttgcctg caggggcctg gcctgcgagg 60
gctctcaaga gcctttccct ctgccctttt caagcctctg cccatctgtc ctgggcatgt 120
<210> 20
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 20
ctctgccagc agtaagagct ggttgggacc acactgagtt ctctgtgacc tgcaggtcag 60
ctcaccttgg ctcacaggct gaacagaaat atactcagaa accgattttc ctatctctct 120
<210> 21
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
gcctggaggg tggtggaggg ctggtttggg gaagagtggg ctagtgcatt acatagggtg 60
ggagccaaac aggagctgcg ccggtggaag catgtgggag ctagaagtga cgtctagggg 120
<210> 22
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
tgggggcgag ctttcaccat cgtgatggac actgaaggag ctccccaccc cctgatcagc 60
caggaggata cacacggggc tgaggtgcag aatcaggggc tcctcaggga actgcagctg 120
<210> 23
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
ctctggtggg gggaaggttg ggagcttccg ttttggcttg gcctgggctg ccctaatcac 60
caccccaccc aattccaggg actagcataa cgaagtgaca ccttggcacc tgtggcacag 120
<210> 24
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
cctgagacac tattcagtcc tgccttcctg cccccttggg agtccctggg gctctgtgca 60
ctcaccaatc atgatgccgg agaaagccag gaccagggcg gccacgaggg cagaggtcac 120
<210> 25
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
gatcagcgag agtggcaggt gtggctccgg ggtgggtgac actggaagac aggtcccact 60
ggggtattga caaccacacc aggtctcctt tgagttggtc ccaagtcaga aataacctcc 120
<210> 26
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
cccactgaga caaaaactac ttgctccttc ccatcgctgg agaccttgtc acacttttct 60
tttcagggga ccatgagcag gaccagactc atcccgattt gacaggcttt tagcagtagc 120
<210> 27
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
ttttgggtga agggaaattt aacagggatg aatatcagtg gcgggatgtt ggtttgaaac 60
atgagctgca caactatggg gtgaggatgg aaggtgaaac gaaagtggat ctggaagaga 120
<210> 28
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
tgtgggggcc gcaggtgacc aaacaacagg gtgcgctgta agtaacaccc tctgaggcta 60
agttacttcc ttgtggccct gaggcactgg gctcacagtg gggaggggtc ctgtgccctc 120
<210> 29
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 29
tgctggccag accacacgga ggtatcgtgc ttggtactgg acactacatt tcaaaaggga 60
catcgatagt ggaacacatg cagaagaggg acactggtgg ggcgggggaa gagacacaaa 120
<210> 30
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 30
actgcatctt gtatgaactg ctgcaaacaa atgggttatt tagtctgaag aagagttaaa 60
ggaggaggat ggggccaggt gcggtggctc acacctgtaa tcccagcact ttgggaggcc 120
<210> 31
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
aaaaaaaaaa aaaaaaaaga ggaggagaat gggagcactg tcttcaaaca ctgcacatgc 60
acagctgctg ctgtaaggaa acctaggaga ggaaggttaa ggcaacaggt ccccagctct 120
<210> 32
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 32
gaaactgccc aagggaacag agaaccttag gagcagtaag atccctgtca ctgggcatgt 60
ttaagtggag gcaggatggc cccttggtgg gggtggtaga gggcttattc tatagtagag 120
<210> 33
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
gatttttaag actccttcag gagccatgac ccacctttca cacagtggtc agagcactcg 60
agctgtggca ggtaggggag ggacagaaag tttacaaaac cgaatccagg gtgttctgga 120
<210> 34
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
acccagaaac catttgtggt catgggccaa atctcaggct atgggcccct ctgcctcccc 60
tggccctgcc cccttaccaa tgcaggagac gccatcctca gccagcaccg tcccgtggtc 120
<210> 35
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
cgtgagcagg agggctcgcc gatttgccca ggtgagccca tacctattgc ctgtctgggg 60
aagattgaaa ggccaaggga catgggggca cagggaggca ggtgacactg cctcttggcc 120
<210> 36
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
caaccagcac agagtagact gggtggagtc ctgagcccag ggccaggagg tacagctgtg 60
tgcacagaag aggcctggga gagctcacag tgggcagggc tgggggctcc ttgggcctct 120
<210> 37
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
ctttttttcc cctttccatt cttggtatct ttaaaatgta ttttcaaaaa tgcaagagca 60
atactgggta aatctgcata tggtgactcg gaagaatctt cctggtggat tggtgagagt 120
<210> 38
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 38
ggcttgcaga gatgttggtc ccacgtgact ccctttgtgc aaaagcagat cttccctgac 60
agggatctgc aagtgtgcag agatcacagt gctgtccaca gtggtgccct cagggggagg 120
<210> 39
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 39
caggaaacgc ttccactttt tactttttgt aactaggttt gctagaaagc gtatatttat 60
atttgtcttt ggaaaataat gattagaaat agaaggcata aaagtaataa atattcatta 120
<210> 40
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 40
aagaagatga ggcatgaggc atgaggcgtg atcccttcta cccataggcg ccagtgttgc 60
acatcgtggt gttctttctg ggatgtttct cgtaagcact tattaagttg acttgaaact 120
<210> 41
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
gcagcacgca tgttctcgca tgccgctctc ccttgcagag cagcccgtgg cgcctcctct 60
tcaggggcca tctgccagtt ctttcgggtg gttctgtgcc cgcatcagct cctggcagcc 120
<210> 42
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
acccgtgtcc agcccacgag ggagcccacc cttggacata tctaagctcc ccgaccacct 60
ccttggcctt taatgggagt gggatcagag acagaggggc gacaagacag agggccccgg 120
<210> 43
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
ggggtctcat ggggggcggg gcaagagcag gcattgctga agttgggtca cctgccctct 60
gtcaggagca acgggggcct gaatcccagt ccagcctcag cctcactgtg gtctcacctt 120
<210> 44
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
tctccccagt ttgtaaagct gtgaattagg gacaggaacc ctgaacaatt ttgagagtca 60
catgagcatt tggaagtggg ctttgtaggg gaaagtgagg aggcccagga ggctggtttt 120
<210> 45
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 45
tgctgtggtc acttgtgggc ctgggagagc ccagccctgc tctggcccgc ccccatctcg 60
ccatctgtgg aacttttggt ttcaagcagc tgcaagagtt gccaagaggt tgaaagaatt 120
<210> 46
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 46
cagctgcctg actccaggtc ctggcatccc caggagaggc ccctctttcc caggggctct 60
ggtcaaagtc ccagagcctg gcatccccag gagaagaggc tcctctttcc cagaggctct 120
<210> 47
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 47
gctcaaagtc ccagggaggc cccctggtcc tgcatgggct ggggcaacca tgctcccgag 60
tcttgtggct ggggttcagt gctctgacca ggggacacca gggcagggtg gacctctaaa 120
<210> 48
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 48
ccctgtgcac tgagggagga gtgaggggtc cccagagcac tgctggggca gctgggagca 60
gccagggagg agcctgggag acattccggc ggctttgttg tctgagtgag ggaggaaagg 120
<210> 49
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
ggagtaaagg gttgagtcag ggcctgcctg gggcttttcc tgccaccaaa ctgaacctcg 60
aggccctggg ttgccttgac ctccagctcc caggaacagg ggcagctggt aacatgtggc 120
<210> 50
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
ctgagtggaa cggggcaggg cagggctggg gcaggtgggt gggtatgaag gctctgaggg 60
gtggaggaca gggtgctcgg gggggctgct gtctccaggc ccagttgggg gctgttccag 120
<210> 51
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
gacttaggct gtgtgggaat ctctaccctc aggccattac aggccggtcc agctgcctgg 60
ctaaggtgtt cccctgtgcc cccctagatc gggaaagtct gtgtggaaaa ctgccaggca 120
<210> 52
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
aggcaggccc aggcccagct gcttgtaaca gtggaggggt catgtgagtg cctgctccag 60
ggagggaggg tcggggtcct gggggcttct ggagcctggg cctcctgccc tttgagaaaa 120
<210> 53
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 53
gcagtacagc tgcaaggctt agctggggag tggggaaggc atggaccagc ttcaccctga 60
gtgacccagc agtaaatggt tgctccttcc agataacata caggaccttg ggtaaatttg 120
<210> 54
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 54
aattttgggt aaacaacaag cagttttttg gtataggtgt gttccatgca acttttgcag 60
cttctcgaaa gacacacctc taggtccatc catgccctct taggaacatg ctgacacagc 120
<210> 55
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 55
tgccattcat gccattcatt gtgtatctga aatgtaggtc ccacagggcg tcgttggctg 60
aatctggctg cactcacatc cttcctcctg tacttacccc agcccaggtg acccctgctt 120
<210> 56
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 56
tgtgaccatg atgtcctgta ccctgccctg cgccctgtgc tcctggcact gtctttgctg 60
ccctgggtct gtcactccgg tccccttggg ctccatccgt gggcagctca gctggtgctg 120
<210> 57
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
ttccctgtcc ttgggcacta gctggacgct gggcccaggc cagccccctg tgaccctgct 60
tgtctgccac ctgcagatgt ggccgaggag gcgggctgcc ccctgtcctg tgcagtcagc 120
<210> 58
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
tccgtaagca gggtttaatc agggcatggg aacaggtagg agatagtagg ggaaacctgg 60
atcccacagg cacttcagcc agagttgcca gggctgtcag ttctatgcat caagctgagc 120
<210> 59
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
ctcctgtgca tttcagcatc accctagcca tggggaggga gcaggcaggg tcagggacag 60
ggggaaggca gggacccaca gactgtccag gcccctgctg aggtcccaga agtcggcaca 120
<210> 60
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
cacagatttc agaagcagag aatggtcagt agggacactg acagggaaag tgcggccctg 60
agccagacga gggacactgc aatgtgcggg tcaggccacc aggctccagt tttggaggca 120
<210> 61
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 61
gagtcctttg ttcagatgaa atgttaggag ggggcctggc ttcacccatg gcttcagaaa 60
ggcactgtga ccaagccctg cccggctaag ccaagctgct gggcccctgg cctcactggg 120
<210> 62
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 62
cctatgcttg cgacaccagt tggggagggg gctccttggg acactgccct ggaaatatgg 60
gcgcctgggg tggtcaggcg ccccaggagg ctgagtgggc tacgtctgcc ctcagggggc 120
<210> 63
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 63
gcccgaagac atccagggtg agtgggtggc ggccgggacc accaccacct cccagcccca 60
cagaggtctc aacagcacat ctgaggtccc aacaagggag gaaattgctg ggaggcgagt 120
<210> 64
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 64
gggccccatg aaacttccct ccctccctct gggcctctgt tactccaccc aggagagggg 60
ccagggcccc tgtaaagtgt cttctggcca taagttctat gatggacagg ccagaaaagc 120
<210> 65
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
agttcttcca ccaaacaact tgtcagcctg acaagtcact gtccctgtga ccatgcagct 60
gggacccacc caggaacaca tttcaaggtc agcaggtatg gtgggttgca cagccacact 120
<210> 66
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
gactacactc aggggtgctg ttctgcctga gcatagggac acgtttctgt cattgagttt 60
tctggtatta tatagcccta cgtccctagc cacttagcat tttcataaag aaaatgccaa 120
<210> 67
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
agacatttgg aacagaggaa aattttgacc tcccctgcca gccctccagt gccagctggt 60
gtaatgagca cagcctctgc tgtgtgacct tggcaggctg ctcagcctct ctgagcctct 120
<210> 68
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
gtctccatct gtaagagggc aatagtggtc taggaggggg cagtaaatgg cagtacccat 60
gctcgatggg gtgttctcag gccttcccac acctccatgg ccacttccca gctggcgcgg 120
<210> 69
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 69
acacggcagg ctggagagcc atgaggcaga gcatacgcag cctgtaccca gtggtgccga 60
gcctctggcg gtgccaagcc tcacaccacc cccacccaca gatccactgt gcgacgagct 120
<210> 70
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 70
gagaaccagg tctccgtgga tgccttcaag atcctggtga gggtccctgc ggggcaggga 60
agatcccctg ccctccccag ctgccttcca gggagggagg ccagctgggg agacagaggc 120
<210> 71
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 71
catcctgtga ggggctgcca acgctgggca gacgaggcct gtgttctgcc cccatttcca 60
tagggcgctg tgtggggaca gtctgtgggg tgggactgtg atgaggtgcc gttcccatct 120
<210> 72
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 72
aggtgagagg cagtggtcag ggtcacagca tcgggcaggg gagcagcagt gtggatggag 60
gggcactgaa gtcagaaggg ggtgcctttc tggggagcct ggcctgcagg tctgcatgtg 120
<210> 73
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 73
ctactcagag cctccaggct gtgccgagta tcctggagcc tccttgtccc ggccaggcag 60
gcctctgccc tctcctggtg gtggcctgcc ccttcagtgt tcctactagc actgtccagg 120
<210> 74
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 74
gcgctggaag ccaagcccag ttctggaagt aacagaggct cagagccaag ggtgtgagtg 60
aacggtgagc cacgcagctt atggtggcgt gaatagctcc tcggcaggag cctccaggga 120
<210> 75
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
ggaagctgag cacccagtgg ccacagggcc ctggcagttc ccatctcagg ctgggaggtg 60
gcctgggatt cctgggaggg gccatatccc acagtgcagc tcagcctgag gcctcggccc 120
<210> 76
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 76
tggagcctcc gttcaggcaa cacccagccc tcggtaaggg tgtgagccaa ggaggccttc 60
ccagatgtgg ccactgccgc ttccccacca gctttcctaa ttggtggtcc ccatcctggc 120
<210> 77
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 77
ctggctgcag cttagcctca tggcagggct ctaggatgag ccaccagagt ccttcataaa 60
cccagtgggt ttgtgtgagg ctgcccagga aggccgcact ggtctgggct gctgctggca 120
<210> 78
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 78
gagaccacca ccctaacccc agtcagctcc agagtcacac tcatcagcac caggtcttgg 60
acccatgact caacctcagt atttgagagg atcaggttga tgtcgccctc atgtgcttat 120
<210> 79
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 79
tgcagtctct agagtgtggt aaacaggttt ccagtgccag ctgtggaggt gacagcggca 60
gggaagccat ggcagtgtcg acactgacct tgactgtggg ttcccaggga atgtggggcc 120
<210> 80
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 80
agaccaggac agcccaggag caggagacct ggggtgacgg atgcccagag ctggcacatc 60
aagggagggt tcctggatca tggcaggctt tggcctccct ggtcagagtt caagtactgg 120
<210> 81
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
gggccagggt gggggtctgg gaaggcatcc ggagcagtcc caagtgggcc caatgtgtgg 60
atagaacttt ggtgggaggg cagggtggta gtgccagcag gcagggtgag cgggtgcgtg 120
<210> 82
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
agggccagtg gcagcccttg aggagcagtg cttccacact ctgaggcgga acatggtggc 60
gcctttcttt gcaggggtgg ctatgtagag aagttgtcct ggacacttcc actgtagtca 120
<210> 83
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
gaggtcctgg gctgggcctg gtgctcattt agtcctgggg caggggtcag gggagacagt 60
agaccaggaa ccagagaggg tcgaagtact gagtccaagc catgctgtga ccacacctgt 120
<210> 84
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
catgtagcag ctttcagggg cctggctgtg gggtcctgcc cagggcagag acaggcagcg 60
ttgccgctgg ctcagatgac agccggttct ctgcacattg gaacttgtcc atggggcctc 120
<210> 85
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 85
ctttaagggt cttgccttct tcctcccctg tcatcctcac acttttcccc cctcttctcc 60
cccttccctc atttccaaca taggaggatc caaagtggga attccctcgg aagaacttgg 120
<210> 86
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 86
tttatgaagc cagcatcatc ctgataccaa aactgggaag agacacaaca acaaactttg 60
ggccaatata cctgatgaac attgatgtaa aaatcctcaa taaaatactg gcaaactgaa 120
<210> 87
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 87
tcatctaact accgttgaca ttcttcatag aattagaaaa agttatttta aatttcacat 60
agaatcaaag aagaccccat gtagccaaga caatcctaag caaaaagaac aaacttggag 120
<210> 88
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 88
ctcagtgggt ggggcttccc tgcagaatct tcagtaactg cagccagagg ctcagggaca 60
gaattcagat ctccctgggc ctgagcccct agggggaggg gtggctgcag tctctgcgga 120
<210> 89
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 89
cagaactggg cggaggatca gatggatgaa ctgacagaag taggcttcac aagatgggta 60
gaaaaaacta tgatgagcta aaggagtatg ttctaaccca atgcgaagaa gctaagaacc 120
<210> 90
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 90
agaaatggat acattcctgg atgcatacac caagactaaa ccaggaagaa gttgaatccc 60
tgactagacc agtagcaagc tctggaatgg aggcagtaat taatagacta ccaaccaaaa 120
<210> 91
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 91
acatgcacat gtatgtttac tgcagcactg tttacaataa caaagacatg gaaccaagcc 60
aatccatcaa tgacaaacca gataaagaaa atgtggtaca tatacaccat ggaatactat 120
<210> 92
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 92
gaaggagcag acacccattt ttgctgctct ccagcctcct tgagttacat atccaggcgt 60
gggagcaaat cagatgaata aggcctaaag tgaaccccag caaactgcag cagccctaca 120
<210> 93
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 93
ccaacagact tagcctctcc tcctggtagt tctgaggaat ctgggaagcc cagacaagtg 60
ggctttccca cagcaaaaca caccctctcc accaggggac aaagtgcttc gttaaatggg 120
<210> 94
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 94
tatgagactt cataaaaaga ctgaacctat gatagcttgg agtaccagga ggagatgggg 60
aaaatggaaa cacgtgggaa aacacccttc gggatattat ccaggagaac ttccccaacc 120
<210> 95
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 95
ctgcgggagc cagcgaggct ggacagcttg gtcccaagac ttgtcgccac aacccaacac 60
accagctgtg gtagtcagcg gccagagtgc ctcttcaggt ctaaccctca cccatccttc 120
<210> 96
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 96
aagtgggcaa aagatatgaa cagacacttc tcaaaaaggg atttatgcag ccaataaacg 60
tatgaaaaaa gctcaacatc actgatcatc agaggagtac aaatcaaaac tacaatgaga 120
<210> 97
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 97
acaaaaatca cacgattatc tgaatagatg cagaaaaggc ctttgataaa attcaacatc 60
aacatccctt tatgttaaaa actctcaata aactaggttt ttatggaaca tatctctaaa 120
<210> 98
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 98
ctccttgagg gaggggcagc agccagcact gggactccca actgcctaac acgctaatct 60
ccctgggcgg cggggagaaa tgcagcatcc atttctatag ctccaggctg tgcttttccc 120
<210> 99
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 99
gaagagggaa gtgactattg aaagaaaaac aaataagcag aaagtgacaa caacagcatc 60
atcatcaaca acaacaacaa gggcccccac aaaaaaccca tacaagggtc agcagcctca 120
<210> 100
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 100
atgtcctccc aaaattcata cccactcaga atattttaat gtgaccttgt ttagaaataa 60
gatcttgaca aatgtaatca agttaagatg aggtcatagt agtttagggt ggaccctaat 120
<210> 101
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 101
aaggtatcta gattcgctca tcaaaattga ctagaaggct ggcgtgaccc acagagagaa 60
ggaagagcag tgagcccacc tgagaaccac acggggaagg ggaaccccct cccctccagc 120
<210> 102
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 102
gggaaggcag gaagattttc aatctcctct tgggttggaa gagtactgta aaatagcaac 60
atttttgtct ccccaccctc cacatatata gggtgtacaa gtttgtgttg gtattggtat 120
<210> 103
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 103
ttgtgtttga gagagaaaga aatatctcat ggctttgcag tattgtgtgt tttatgtgac 60
acggtgatgt ggaactttgg gttcctaatt actttacttt actattctac aaccttttac 120
<210> 104
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 104
ccagacagag catgatggtc cacttctagg caccttaatt ttgataaaaa tatatatgtg 60
tatgcattgc ctcaacactt ttattgtagc ttgttgagtc aaagttagta ggtattaact 120
<210> 105
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 105
tcttcagatt gccaatttgt ataaaaagct actgcaaatt agtaataaat gcatctgggt 60
ttataagctt ttctcttagc tatttcacac atgtgacatc ttacgtaaaa caaaatttac 120
<210> 106
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 106
tatgaccaaa aaaatcaacc ccatcaccat aaggttaact ttaaaaagcc aagtttctca 60
cttggaatac tacaaggggg gaaaatgctg ctatgttaaa aatgctttca atttttaata 120
<210> 107
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 107
gagaatcttg agtagcagat atgctgagta tttgctcaaa ggaaagtcac ctctaaacag 60
ctgatttaga tcacctttgt gtctacttca aatcctttgt ttttacacct tgcattataa 120
<210> 108
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 108
atttctgaaa gcaaggccta tgatcattta attccttttt gcatagatcc taatataatg 60
aacctttgca taagacactt gaatattttc cttaattatg acggcaacaa aagttcaaaa 120
<210> 109
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 109
ctcatctatc ttaagaaggt tttgtgggca atcatcacac aacttctgaa atggagtaag 60
attgacttaa ggctgaccac gtaacacaag gctctcaaaa ctcctctcct ctcaaacact 120
<210> 110
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 110
gcctccaggt caaccaacca tgttaccatc tattctcttc aatgtaatat ttttctcctt 60
ctttcatttg tttttgagct acatgacaaa atactggaaa ctaatggatt tttctgtatt 120
<210> 111
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 111
tttcttaaaa ccacataatt ttccatccat cccaggtcac atttgagagt ggaagaggaa 60
gttattaata attatgccag aaaaactggt ataaaccatg actgtcttgg gcaatgcgga 120
<210> 112
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 112
attcataggc actctcctta ctgttgccca ccctttgcct aggtgctcca taatgatggc 60
caaagctaca tactgtattg catagcaggc attagccagg cctactccgg ctgccagacc 120
<210> 113
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 113
cttttttgat tctttaatct tctatgccag actataaagg aaaaaatgac ataatttaca 60
aaataaaata ttgcttcttt tttcctttag gaaatgttaa cagtgcattt gaagtgtttt 120
<210> 114
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 114
tatgcaagaa cactatcagt agaatagtta tagaagtttt cataatttga ttttaaaata 60
tcttataaaa aactaattaa atccaggtaa aaagccatat ataagtacac aaatcatact 120
<210> 115
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 115
acaccatttt taatctcact tcttaaaatt taatgtattt cttactgttg ttataaacaa 60
attaaagtat cttaatatta aaacttattt gtcaaaatca cccataaacg agtcaaaata 120
<210> 116
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 116
aaccaaaaca aacataatta ttagtgaaac aaattcattt ttgagttcag tcacatccag 60
gttatattcc tcagtagaaa agaaaatgct ttcagtataa tggcaatttt tttttttttt 120
<210> 117
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 117
ttttttgcag agtacaagag tgtttatcat tgttgttcat atacacatac tcgaatatgt 60
agaaaaagta ttggaacgaa gcttaccaaa tgttagcagt ggtctgccct agttgtagaa 120
<210> 118
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 118
tcatgagtaa tttgtactgt cttccttata taataagcag tattagtttt ataataataa 60
aattttttta atattaaaat acaattatgt caataggatt gtcatctgta ctggactatt 120
<210> 119
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 119
ggctcagaag ttgaaagcag caagcatgct ttgagagatg atcattaaaa tttgaactta 60
atttcacagt agcttctatg ttcaagaagt tagagtttta ttcacatgaa attcaatttg 120
<210> 120
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 120
aagggctaca cagtgaaaga tcacaagtac cttttgctaa attaattttt ccaacttgaa 60
atgttctcac cacctcgctt cacttttaaa ggcccaatgt aagcccacat cctcaatgat 120
<210> 121
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
gccttctgaa acctcagcaa cctctactaa tctttcatat ctgaacttga aagactaatt 60
gcgcctctct gtgagtgctt atcctatgca ctgtcttact tatttcatct atttttgtct 120
<210> 122
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
tttgttatta tttaacacat atcttgctat ttgtcttccc acacaggcat gtcttaactc 60
cctcattaaa tggcaagctc ctgaggtcag cagttgtatt ttacaggttt acattttccc 120
<210> 123
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 123
ctactcccag cagaaggcta atgttatgca ttagatatga ctgctcagaa gcaaaggctg 60
tcaacactca ttttaaaaat tgtctaattt tatgtttata actgatgagt gccctcagca 120
<210> 124
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
caatacaaat tttaaaataa ttgtgactgt tttgttattt taattactca atcttttctc 60
atgcacacac acgctcttac tagtaaaacc acatgcactg ctgctaaaag caaagaaccg 120
<210> 125
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
aattagctta gctagactca aaaattcgtg aatttcaaac agaataaaga cctttaagtc 60
ctgagaaagt cagatatcca taggatgtct tggaagcaca ataggttgtc tgaaattatc 120
<210> 126
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
tcatgttcat ccactagagt tatgggttta aacaatggtg atgtacaaga aagcaagcaa 60
aggtcagctg aaggattctt catgtaattc tccttcccag taggaaagca gacaagcagt 120
<210> 127
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
caagcctata tgccaactgc caaatatact gcagtcatat gcgtaagtct ggaggggctg 60
gcttagtggc acaaacatca gctgtgcaat acttagactc tctggaacca caggttaaaa 120
<210> 128
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
tcctggcagg gtcagctgaa atttttagct ttccaagttt gctgaaggag tttcacatat 60
tgtctgggtg gaatctttga aaaaaatctc aaatcgagct tcaaagtgct ctcataaaga 120
<210> 129
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
ttgtgactcc aatcactttg cagaaagaat aaagacttag aacacagtaa agtgttggct 60
gtctttatcc tgagagttca actttatgtt tgtgactttt ccatcaactt tcccttgtaa 120
<210> 130
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
cttaccccta cttgtgaaca aaacaagcca caactttctt aatatgtgtt tgagtgttct 60
tttctggtaa gaatcatgcc cttcttttaa tgattcagtg tgttggggac tctcaatatt 120
<210> 131
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
agcaatagat aggggtccat atatcttata agcaaactcg gacagtttgg ggaatgaaag 60
aaagtgctat tattatttgt gctgaaacag aaataaacaa gaatgtatga tcactgtgat 120
<210> 132
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
aactctaaaa gtccaatcag tacttgacca ttccctttga ggtttttact gcattttatt 60
atcattaaag taaatcttct tagaaaaaaa atagctttct atctacctaa cctaattttt 120
<210> 133
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
ctgccttcct ggtcatttat aaatgagcag ggatttgaaa taagcagatc agatgtctga 60
aagcaagagt cgatcccaca agccagaaat ggatcttctg caaaagaggc cctactcatt 120
<210> 134
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
ccaaaccagc ctgtctgctt agaaaccaaa actatcccaa tcaaagattg tcactggcct 60
cccattaatt ctatactttt ttctgtaccc agggttattt ttcttttatt tattttaaac 120
<210> 135
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
cataagccac aaaacatttg tatgcagcca atcagaaata aatactgttt taaagaaacc 60
taaaagttgg tgcccttgca agtagtttta tctaaagtat aacatgaaat gtacatgtat 120
<210> 136
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
attttccata aaatagttat gcctagatca gaaaacaatt cacagaagtt agtggttata 60
aatgtatagc caaaggtaac aatgtaaatt ttattaactt aatcaggcaa tatttcatgt 120
<210> 137
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
gggagattag atcaactaag agataaatca atcaggtatg attaagtaaa cagtttgttg 60
cctattttaa aaattctatt atacttacca aaggtcagtg ggattgtaac aaccagaaat 120
<210> 138
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
aatattccaa ctataatagt aagtatgaaa cttgtttctg gtatccaaaa atcatctaat 60
aatataaatc agaaaaagaa attaattcat agataaaagc taagttgccc cagctctacc 120
<210> 139
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
taagcacaca gagtaatata gcagagctag ctactactgg atttttgcaa gccagttgtt 60
agaccgttgg tagcttgaaa gcatccatac agacagtgtt tatgccatgt gaaatcaaaa 120
<210> 140
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
gcaaggtgtt tttgcttttt tttgcagaga gcaacaaacc actggaatat atccactcaa 60
tcttctactt taaaatgact taggaaacat atatatcttt ttaataaatt taggaccaag 120
<210> 141
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
aaatctcagt ctttggatac taaatagttg gcattattat tttgtataat atcttcatac 60
atatttaatt tcatggatat taaactgagg aataaacatg gtaatttctc ttaggtagtt 120
<210> 142
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
tcagttgtgt agaggaaaca aacacgtagg ccagagtctg gcaaactatg gcatgaggac 60
caaatctggc tatagcctga ttttgtaccc cctattagct atgaataagt tttacctttg 120
<210> 143
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
taaagtgtta tttttaaaaa gaaggagaag gaaaaagaga acagaaaaag aagaaaagaa 60
gaacatgcta cagagatcac atgtagcctg caaagtctaa catatttact atttggttct 120
<210> 144
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
tttcagaaaa agtttgctga gctgggtagc aaacttctgt acacaactaa ctagataact 60
aactataata taaacaagaa tcatgtaagt tttatatagc atgtgagtgt ataaaatgca 120
<210> 145
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
ataattcatt tgactaagga gcatcaggta agcattatga tgagttcatt taagtcttga 60
aggatgagaa gaatttcaat tggcagaaaa taaataatag gaaatcacag ctaaggggca 120
<210> 146
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
cagggtaggt agtggggacc tgtattctag gctgaggcaa acacagggcc aaagactaag 60
tgacataaaa actttttact ggtctgatat ggctacagca cagacataaa tattggaaaa 120
<210> 147
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
gataaaccac aattggagga taaattacag aagacctcac atgccacaaa gaagtttgta 60
gctgattctg aaagcagtaa atggagagcc attgtgggta tttcagagag ggattaagta 120
<210> 148
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
atccagcctg tgcttcaact aaatttaact gtcagcagta tgcgtaagtc aagggcacag 60
gcaagaaatg ataccttaaa ttaggaaagc caaggtggaa agaggaagat gagaactcat 120
<210> 149
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
cagagaaaaa tcaagatggt aaaatcaaca ggacctgatt actgagtaga gggaaaaatc 60
acatatggca ctgagatttc ctgcctatgt gattagaaag acaatatatc ataaactaaa 120
<210> 150
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
ataaaataca cagtggaaga ggtagatatt ggggaagaaa caaaggtctt aggcctgtta 60
tgtataatgt aggagtggtc ataaggctgg tataatgtga atagaattat atggctggat 120
<210> 151
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
agaacagatt gtgaacagcc ttggaagcct aattaggagc atggaattga tgtggtagga 60
attaaggggg tggctgatta ttattgggga gaaaaatgac atgatgataa tgatgtttta 120
<210> 152
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
gaaagatcac atcacatgaa tggaatagtt taatagtttg gataataaga ttatatatat 60
atatatatat atatatatat atatatagtc accattatct attgctaatg ttaagaatgt 120
<210> 153
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
actgatattt attactgaac ctttaggtaa taagctagtg tgtagacaga catggtaaca 60
tacctccaac taatataata ttctcactga ttccactata ttcaccaaac cctagattat 120
<210> 154
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
ccaccttaaa ttctggttct gtaaattatt tgaagtgctc tttctgcaaa aaataataaa 60
tacagaaaat atacatgaca atatacctgt tatttctagt tgttcataga tcttcttagt 120
<210> 155
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
gactgaacgc cagagagtgt cctttggcac aatttaccct tgacctcatt cctctcccat 60
aatctggcaa cacagatgct aacagcatgg ttttatagtg atcctagtga ggcatggagc 120
<210> 156
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
caattaactc agcccaccaa gaaaactgtg agtaatcagt ggtgaagaga gagctgagta 60
cctttttcct atactaacag catgggtaaa ctttctccag aattccaaac ctataagaca 120
<210> 157
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
attattccat gatttttaac tgggaagttt ccattttctc aattttcagt cttttacatt 60
atttgtagtt tacttatgcc atatttcaat aaaatacaga atacaaaatt gataaggaaa 120
<210> 158
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 158
gtgtgacagt tcagctacca aagttatcat tatatctgag attctgaggc tcgttgcttc 60
cttttgtatt gacctacatt ccacctcaaa gtcaatccaa ttgtttttca tttgaaacat 120
<210> 159
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
ggaaggagca caagggctgg gttcaaaaga cataactgga atgacttttt acagaatcat 60
caaaggcaag ctcaaggtct tccattatgt cttctctaac aatgaagaat atttcaggtc 120
<210> 160
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 160
tgaaactttt cagaccgcaa tagttttagg acttggtgcc aatccacggt aagcttaggg 60
atatgtggga gaatctggtt ctattcctgc taattgactc atccttctga agaccaacag 120
<210> 161
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
agagtgaagg ctggccctca taagaggcag ggaaagggta agaacatcgg gagcagcagg 60
gcagcagagt tggtcacagc tgttccccgg agctgagggt ccctttaagg accatcctga 120
<210> 162
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 162
gcaaagcatt aggacagagt gtttttacac attttgtgac gtctcacctt tagcattggc 60
catttcactg tttaaccaaa caactctgat ccaaaagaaa gaggcccttg agaatttgtg 120
<210> 163
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
aagtggctct acacaatggc cattgagaaa agaaagaaga attctgatgt tggttgagca 60
ccttctattt acaacatact gtactggggc tttacataac gttgttttaa ttcactgagt 120
<210> 164
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
ccacaacagc ttaaagagat aagatagata ttatcatctc cattttacag atgaggaaac 60
ttaagttcaa aaaaggaaaa gaacaagcat ttaaacatgg tttgtgtact ggattgagtc 120
<210> 165
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
ccaatgactg gcacttgcat aagaagaggg aaatttggac acagagaaca ccttgtgatg 60
caggaggtga tgatgacagt gatgcaccta cgagccaagg aataccaagg actgccagta 120
<210> 166
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
aacgccagaa gctaagaggc aaagatggat tctgccctag aaccatcaga acaagcagtc 60
ccgctgatac gttggtttca gacttctaac ctctagagct gtaagaataa atgtctactg 120
<210> 167
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
tttcaagcca cccagttggt ggtcctttat tgtggcaacc cttggaaact aataaggctt 60
gttcaactcc caagttcatg cactttgcac ttccactacc ccatcagtga gtacacagag 120
<210> 168
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
tctaactaga gtgtcctgac tgaaggactt attttgagac actgaagtta caacaatcat 60
tcagtcatcc tatagtctat gtcctgacag tggagacctg gagcaaggag tatgcaaagt 120
<210> 169
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
atttagatgg agtcatttta gtagcactta aggtgttact cagggtaaca cacaaaagag 60
gtcctagcaa aaacctgatg tgtgacctga ttgatgaaca tcattacagt cacaaaccac 120
<210> 170
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
caagacagtt ctgcaggtta gaatgtattt cactctgcat gaaaagcagc aacagaaact 60
tcaaggaggt ttcaattaga aataagggcc aagggaattc tctagtatga acaggaacat 120
<210> 171
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
tttttaaggt tatgggaatg ccagagccag atgaagcaaa aatagatcta aagatcaagt 60
tgccaggact ataattatgg aagtgacttt tgtccttgga acttcttaaa agatggtttc 120
<210> 172
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
atagtcctgt gagaggagtg tctttggaat ttggaattct cccctcaagt tggcatgaaa 60
tcagtccttc taagccagtt atggttcacg tgctccccgt actggccagg tctgaggctg 120
<210> 173
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
ttcatcagtg gctggttttc atcgacggtg tgttttcagt ccctgggaac acattgcaaa 60
ctgtaatctt cccaatgaat acagaatcct gtgtgacctc tacacttcag accatttcag 120
<210> 174
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
aacctgacct aattttacag tatgagaaat atctacttca tcaccatttc tcactagaga 60
gtattcacct tgctaaagca aattatcatc aggaatttac atgccatcat tatttaacat 120
<210> 175
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
taaacatcag ctaagctttt taaacaaaac tgatattaag ttaatactgg ttgcatgagc 60
ctatgatcaa acaatttcca taaagtgtca atgagcagtt agagatttct gagaatgacg 120
<210> 176
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
tgatctgttt ataattagca tgccaagacc aacgtctagg aattgagcaa agaacgtaat 60
gatgcatgtg agtcctttaa ctaaagatct ttgtgtttgt atataaatac atttgcataa 120
<210> 177
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
atagtgttac ttttgattac tttataatta tcttcttagt tctttgtcat ttacaagtac 60
tttgcaaaca cacatacctt atctcaagga tatagtatgt aattctacat ccattatctt 120
<210> 178
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
agtaatttgg gaatgcctgg tttatttggg actccagctt tagggaaaaa aagaaaatat 60
tggttgatat gtttgaagta ttaatcttct gcactgaaat ctttctacag aatatattaa 120
<210> 179
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
gaggaaagac aacattaaag aaatattcag aggcggggcc aagatggccg actagaagca 60
actccgttca gaggttccta ttgaaaaaaa ccataataag tgtgtgagtc cttcaccggc 120
<210> 180
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
caaggggagc ggcgagcgag caggctaccc agccggggaa attgcttttc caaagaactg 60
tgcaactcat ggattggaag atcccactct cgatcccatg ccaccgaggc ctagcatccc 120
<210> 181
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
aaccctagaa tgtacagatt cttatggcct ctcagctgga atctgcctaa gcttactgaa 60
ctccctgggg gaggggtgac cagcacaggc ttctgctgcc tgctgtctaa gccctttgag 120
<210> 182
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
agacaaagac acaaaaagcc ctccaaaaaa aatcaacaca tccaggagcc gtttttttta 60
aaaaataaaa taaataacaa aatataccac tagctagaga aataaagaag gagacagaga 120
<210> 183
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
ttttaaatga gcatttgaag tttttattag tgatggattt gatgaattgg tgataagatt 60
aacaatatat ctcaacttga aacaaaataa gtgataaatg aaaataaaga aaactatcat 120
<210> 184
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
gatctttgtt ccttccattc ttatagagct catctcagag ttaaggtata tctttcagaa 60
cactagttaa tttttaatga acttcttata tttttcatcc tagtatctca aatatagtag 120
<210> 185
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
ccaataaata ttttttgaac attcagtaac actattttca tatagttatc accataaaat 60
tgtcatagct agacatgaaa taaggatact gaaagaagaa gtaattttgc ccagttcagg 120
<210> 186
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
atccagccag acccagccag tattatttca ttactgcctg caaggtctcc tatattttct 60
gaatttcttc attcaagttc tagaaataat aatgtactgc ttttaaagaa caatttcata 120
<210> 187
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
tatctctagg attagtgaaa taagtgataa gaaaaaaact gaaacacaga gttttctgag 60
tattgagtat gttagtactc accttttgtc ttaaagcttt ctggaagtga ggtgctattt 120
<210> 188
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
gcccgtcctc cgcctcccga agtgctggga ttacaggtgt gagccaccgt gcctggcctc 60
ctgaacattg cttctgggct ccctggtcca tgggagcatc agctaggggc tttgctgtct 120
<210> 189
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
tggggaagga ggacccatag agtgttttcc tttttttgaa aggaaaagtt ccagcctggg 60
ccacatggtg agaacttgtc tctacaaaaa cacaaaaatt agctggatgt ggtggcatgc 120
<210> 190
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
taatggaaag atgttaatga tatattgtta cgagcccatt attgggaaaa atgtatttag 60
gaatacgtat ggagggaatt tatttattta tttttttgag acggagtctt gttctgtcgc 120
<210> 191
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
atcatgacaa tatttttaga taagaacatt ttaaacattc aacagtaaga gactattgaa 60
aataaatgaa attcattgaa tagaagtaat taaaataata atgtaactct ttaagcattg 120
<210> 192
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
agctggttgt aaggtggagc tcccaccagt cctggctgaa ggggtcattt tcctggggta 60
atggacctca ctcacacagc tattctgacc ttacagatga cttggaagac tccatgatcg 120
<210> 193
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
ctgccagctc acacattcct gtgaaagagc cagggggtgg gtctgaggag ccccatttta 60
cagatgagat gactgaagta ggggtgggga agctcgcttg ctggacattg agcatttgga 120
<210> 194
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
tgagccaccg cgcctggcct tgaaattcta catttctaac cagctctcag gtgttgctat 60
tggtttttgg atccacactt tgcagagcaa gggtttagag ctgatgaagc ctctgcccag 120
<210> 195
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
aaaaagtaga ttttgattca gtcagccctg aaattctaca tttcttcttc tttttttttt 60
aaccaatgaa ttatttttac tctttttaaa taagtgaaat attagcttta atgttttctg 120
<210> 196
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 196
taatattgca gttagagttt tctttctcaa aagttaattc cctggggatc ttgttaaaat 60
gtagattttg gccgggcgcg gtggcttaca cctgtaattg aagcactgtg ggaggccaag 120
<210> 197
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 197
ataaatttca ttatgtgcat acaacatgat attatgggat acatatagat agtaaaaatg 60
ttactacagt ggagttaagt aatatatcca tcatctcaca tagtcgccca ggaaatgttt 120
<210> 198
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 198
catggtcctg agttcaagcc tccatcctgt gtgaactgag cttcagtttt ctaatctgta 60
aaatgggaat aataaagata gtacatcagt gttgtgggga ctgaactgac ttaaagcttt 120
<210> 199
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 199
gtgggggcgg gtgaagggcc caaggcctct aggccagaca gctaataagt gtccctccta 60
tgtgcagaga ggtgttaatg attgcaagtt ttagctttgc aagttttagc tttggagtca 120
<210> 200
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 200
accctgtctc aaaacaaaaa aggaaaagta gcagcttaga agtggggatg gggtgggagg 60
gggcatgagt gggcagagat gtagttggga aaccaagaac aagtccctgc ttcagtgggg 120
<210> 201
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 201
attggtagct ctgggttttc tgagctccag cctgaacgtg tcctcataag ctcttctctt 60
ttctgcaggg catggtgggg gtggggtgag ggtaggatgg gtggcaggac agggtgggag 120
<210> 202
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 202
tgtgggactt ttcctagagt gcccttcttc cttctcaaaa aaaggggaaa caaaagtaat 60
ggattaacct attccatccc tgagagcccc tggggacaag ctgtttgctg ctttgaagtc 120
<210> 203
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 203
agctgtctgg ctctggagat ctgggtacgg aaggtgtgct gggcaggcgt aggcacaaag 60
ctggagggag tggtggcttc accagccagg agggtgacca tgccttgaga cttggatttt 120
<210> 204
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 204
gacgatgagg atgtagtggg gcccgggcag gaatctgatg actttgagct gtctggctct 60
ggagatctgg gtacggaagg tgtgctgggc aggcgtaggc acaaagctgg agggagtggt 120
<210> 205
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 205
gtcatggagc actggggatg ttggctgatg tgtctccttt ctttatctta gatccgagag 60
actgaggtca tcgaccccca ggacctccta gaaggccgat acttctccgg agccctacca 120
<210> 206
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 206
caacactaaa agtttcatgc ctttctctct ctccccccat cccacccccc tgcagagaga 60
gacaccaaag ggaagattct ctgtttcttc caagggattg ggagattgat tttacttctc 120
<210> 207
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 207
ggatttctct actttttcgt gtgctccctg gatattctta gtagcgcctt ccagctggtt 60
ggaggtaaga atgaaagggt gagaggtctg cgggtgaggg gcattatctt gaaatgtggt 120
<210> 208
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 208
ggtaagaatg aaagggtgag aggtctgcgg gtgaggggca ttatcttgaa atgtggttcc 60
gagagtaaaa ctcagcaagc cctctccagc tgcagcctcc tggagtgttt taggactgga 120
<210> 209
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 209
accgacctca gatcatttat gaagtctatg ctttccgtta tagaagagaa aactgaggcc 60
tatacaaagg gctatgactt ggcccaggtg gcttaggcca ggagctgggg ctagactatt 120
<210> 210
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 210
ccaagctatc cgccagttga gtttgtccac aaaccccagg caggaagttg tctgacatac 60
agacttagct gaggaattcc tatatcctcc acaccagaga gaattctgga atgagaaaga 120
<210> 211
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 211
gtgccctttc aaatatgggc ccttgctggg ggaaggacag ggccccttct ctgggctgga 60
ggaatctgca tccattgtac ttaccttcac agcccttagg gctgcgtgtg cccatgcagt 120
<210> 212
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 212
gtgagaaccc aggagtgaag tcaccatgtg cttggttccc tttgtacctc aatggtgatg 60
tcagaaacaa acaagcagca ctccgtctac tgggtgcagg cattttcggg ggccctctat 120
<210> 213
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 213
caaacattct ttcccagagc cctcccattt acggagagat catgaaccag ccctgagact 60
cacagcctgt gattagcaga gtaagattca gtctcagatc tgggtgacac aaaggaccat 120
<210> 214
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 214
ggatttctgc aacccttggt gcctttcttg ggaacccatc tgtgtgactt gggagagttg 60
gggaggtggg cattcatggg agagcatgag gggcaaacac ttcctggaca ttgctttttt 120
<210> 215
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 215
ggttatctca gacaaatcac ctgcatcctc tgggcttcca ccttctttat acaatggact 60
tgggaatgca ttgatggtct caggtacact cttgagaata actctcccag cattcaatta 120
<210> 216
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 216
caggctggtg ctagggctaa gggtggatag agagatctac tcttggagga taaatctgaa 60
ttgggtatac accccagaga catccaggag gctcattgga acccactggt cccagtatgg 120
<210> 217
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 217
agttgaggct gggttcctga gcacagaagg gcttgctgac tgaggagtgt ttgaagagtg 60
ggaggatgca gggtgaagga acaaaagtaa ccaggggctc acagtgggtc gggaaccagg 120
<210> 218
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 218
gcagagagac agagacagga ggcacgtgtg ggcagctagt agatttcctg ccttatcggg 60
gcagcactgg gaagaggcat ggggaagcaa gattccttgg gtgcctgcag cgatggaggc 120
<210> 219
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 219
tggactctgc aacccacagc cagctggcct tggatggaga cttctgttta ctcagtgccc 60
acctaatccc cctcgatcac gttgtgattg tttttgtttg tttgtttgtt tttcccagga 120
<210> 220
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 220
gtggtgtgtg ggtctttagc tctgctcaga ccctggttca gagctcactc actcactgtg 60
tcctcatcat gcctgtcgct tcagtactga ccaagtgcca ggaagaggtc agccacatcc 120
<210> 221
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 221
acatggccat tcacttcctt tttgatctca gaccttgttg gtctaggcct cagttttccc 60
atccgtgtga tggctggagt gagtaaagcc acttgggaag aaggcattaa gcccacagca 120
<210> 222
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 222
aaaccccatt tctacttgag aactcccagt aacaggaagc ttagacttac caaggtgccc 60
attgcttttc tctagaatca gaatcgcaag tgcaattcca aactgtaatg gtgttttttt 120
<210> 223
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 223
caaagggcca ccctgcctgg ggcctcagtt tctgcatcag attcatagaa ggcaccttac 60
atgctatctc caactcctag ctgatgctta aacccctcta agacatctcc aacaaacggt 120
<210> 224
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 224
taggatgttt ttagacccaa agacagagtg ctgtttctat cccggtgctg tctctaattt 60
gctatgtaac tttggacaag tccccttccc tctaggattc agggtcctga agtagaaggt 120
<210> 225
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 225
cagaaaggaa tccttgtaat gaccttgccc cagtgctgcc cgcaatccag tgagggcgcc 60
aaggtcacag ctgctgccaa gagagccttg ggcgtttccc acctcatgga caatgcagac 120
<210> 226
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 226
catcttgtct aagggttcct aagggcccac agaggcctgt caccacaggg tacaggtgac 60
ctctctcata gaggatggta cagcacaggg tgcctggggt agaacctgcc cgaaacactg 120
<210> 227
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 227
actccctggt cccagcaccg cggaatctcc catcctctca gctctcactt ctttctcact 60
tctctctctc tcctgtctgt tccttcttgg tttggctgtc cccctcccct gacccacccc 120
<210> 228
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 228
tccttggagc aaaagcccac tgacgctcca ccgaaaggta cagggagtgg gagctttagc 60
gtgccagggc ttctggaccc tcggggctct cctgaagctg ctgaggccgg ggcctccagc 120
<210> 229
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 229
cctctatccg tattaggtct ttgagagctg gatgcaccat tggctcctgt ttgaaatgag 60
caggcactcc ttggagcaaa agcccactga cgctccaccg aaaggtacag ggagtgggag 120
Claims (10)
1.一种肿瘤融合基因靶向检测探针，其特征在于，包括针对如下各染色体上内含子的不同起始位点或其附近位点与终止位点或其附近位点之间的基因片段按照预设长度构建的、与所述基因片段互补配对的检测探针中的至少一条：
参考基因组是hg19。
2.如权利要求1所述的肿瘤融合基因靶向检测探针，其特征在于，包括针对如下各内含子的不同起始位点与终止位点之间的基因片段按照预设长度构建的、与所述基因片段互补配对的检测探针中的至少一条：
参考基因组是hg19。
3.如权利要求2所述的肿瘤融合基因靶向检测探针，其特征在于，所述预设长度是100bp-140bp，优选为120bp。
4.如权利要求3所述的肿瘤融合基因靶向检测探针，其特征在于，包括序列如SEQ IDNO.1-SEQ ID NO.187所示的187条检测探针。
5.如权利要求1～4中任一项所述的肿瘤融合基因靶向检测探针，其特征在于，还包括针对如下各染色体上内含子或外显子的不同起始位点或其附近位点与终止位点或其附近位点之间的基因片段按照预设长度构建的、与所述基因片段互补配对的检测探针中的至少一条：
参考基因组是hg19。
6.如权利要求5所述的肿瘤融合基因靶向检测探针，其特征在于，还包括序列如SEQ IDNO.188-SEQ ID NO.229所示的42条检测探针。
7.如权利要求1～6中任一项所述的肿瘤融合基因靶向检测探针在制备用于肿瘤分型的检测试剂或检测芯片中的应用。
8.一种肿瘤融合基因靶向检测试剂盒，其特征在于，含有如权利要求1～6中任一项所述的肿瘤融合基因靶向检测探针。
9.如权利要求8所述的肿瘤融合基因靶向检测试剂盒，其特征在于，还含有核酸提取试剂、文库构建试剂、样品及文库定量试剂、片段质量控制试剂、杂交捕获试剂及核酸纯化试剂中的至少一种试剂。
10.一种权利要求8或9所述的肿瘤融合基因靶向检测试剂盒的使用方法，其特征在于，包括如下步骤：
提取肿瘤组织细胞的DNA；
使用所述检测探针捕获目标片段；
对捕获的目标片段进行测序；
生物信息分析鉴定融合突变的序列。
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| CN113373231A (zh) * | 2021-06-23 | 2021-09-10 | 广州市金域转化医学研究院有限公司 | 泛癌种多基因msi位点靶向检测探针、试剂盒及应用 |
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