CN110432386A - A kind of fermentation process of stalk - Google Patents
A kind of fermentation process of stalk Download PDFInfo
- Publication number
- CN110432386A CN110432386A CN201910842097.3A CN201910842097A CN110432386A CN 110432386 A CN110432386 A CN 110432386A CN 201910842097 A CN201910842097 A CN 201910842097A CN 110432386 A CN110432386 A CN 110432386A
- Authority
- CN
- China
- Prior art keywords
- stalk
- fermentation process
- process according
- bacillus subtilis
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention provides a kind of fermentation process of stalk, belong to stalk fermentation technical field, the following steps are included: after stalk, manioc waste, rice husk powder are mixed, the water content for adjusting mixture is 50 ~ 60%, bacillus subtilis and bacillus amyloliquefaciens are inoculated in mixture again, ferment 48 ~ 72h at 28 ~ 32 DEG C.Using fermentation process of the invention, the content of crude protein is 24.85 ~ 26.34% in obtained fermentation material, and coarse-fibred content is 25.68 ~ 27.48%.
Description
Technical field
The invention belongs to stalk fermentation technical field more particularly to a kind of fermentation process of stalk.
Background technique
Accessory products of the agricultural crop straw as pesticide producing fail adequately to be applied always in China, tradition kind
Agricultural crop straw direct returning to farmland, discarded or burning are often caused into the waste of agricultural crop straw in plant.It is fine in agricultural crop straw
It is high to tie up cellulose content, due to the design feature of itself, cellulose is difficult to be degraded, it is difficult to be digested and assimilated by animal.Using nutrient
Few, palatability is poor.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of fermentation process of stalk, using fermentation provided by the invention
Method, by the cellulose decomposition in stalk.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical schemes:
The present invention provides a kind of fermentation process of stalk, comprising the following steps:
After stalk, manioc waste, rice husk powder are mixed, the water content for adjusting mixture is 50 ~ 60%, then by bacillus subtilis and
Bacillus amyloliquefaciens are inoculated in mixture, and ferment 48 ~ 72h at 28 ~ 32 DEG C.
Preferably, the stalk includes corn stover and/or wheat stalk.
Preferably, it is mixed again with manioc waste, rice husk powder after crossing 20 meshes after the stalk is mechanically pulverized.
Preferably, the weight ratio of the stalk, manioc waste and rice husk powder is (30 ~ 40): (5 ~ 15): (5 ~ 15).
Preferably, the weight ratio of the stalk, manioc waste and rice husk powder is 35:10:10.
Preferably, the water content of the mixture is 55%.
Preferably, total inoculum concentration of the bacillus subtilis and bacillus amyloliquefaciens is 10 ~ 15%.
Preferably, the mass ratio of the bacillus subtilis and bacillus amyloliquefaciens is (1 ~ 3): (2 ~ 6).
Preferably, the living bacteria count in the bacillus subtilis is 1 × 109CFU/g or more.
Preferably, the living bacteria count in the bacillus amyloliquefaciens is 1 × 1010CFU/g or more.
The present invention provides a kind of fermentation process of stalk, comprising the following steps: mixes stalk, manioc waste, rice husk powder
Afterwards, the water content for adjusting mixture is 50 ~ 60%, then bacillus subtilis and bacillus amyloliquefaciens are inoculated in mixture,
Ferment 48 ~ 72h at 28 ~ 32 DEG C.Using fermentation process of the invention, in obtained fermentation material the content of crude protein be 24.85 ~
26.34%, coarse-fibred content is 25.68 ~ 27.48%.
Specific embodiment
The present invention provides a kind of fermentation process of stalk, comprising the following steps: mixes stalk, manioc waste, rice husk powder
Afterwards, the water content for adjusting mixture is 50 ~ 60%, then bacillus subtilis and bacillus amyloliquefaciens are inoculated in mixture,
Ferment 48 ~ 72h at 28 ~ 32 DEG C.
In the present invention, the stalk is preferably corn stover and/or wheat stalk, and the stalk is preferably harvesting corn
After seed or wheat seed.In the present invention, the stalk preferably through after mechanical crushing cross 20 meshes after again with manioc waste,
Rice husk powder mixing.The present invention is not particularly limited the mixed mode, by the way of routinely mixing.The present invention is excellent
It recruits to save after the water content for the mixture being mixed to get is 55 ~ 60% and ferment, the water content of the mixture is preferably 55%.
In the present invention, the weight ratio of the stalk, manioc waste and rice husk powder is preferably (30 ~ 40): (5 ~ 15): (5 ~
15), more preferably (32 ~ 38): (8 ~ 12): (8 ~ 12), most preferably 35:10:10.The present invention is to the manioc waste and rice husk powder
Source be not particularly limited, using routine.
For the present invention preferably in terms of the quality of mixture, total inoculum concentration of bacillus subtilis and bacillus amyloliquefaciens is preferred
It is 10 ~ 15%, more preferably 12%.The present invention does not have special limit to the source of the bacillus subtilis and bacillus amyloliquefaciens
It is fixed, using conventional bacterial classification.In the present invention, the mass ratio of the bacillus subtilis and bacillus amyloliquefaciens is preferably
(1 ~ 3): (2 ~ 6), more preferably 2:4.In the present invention, the living bacteria count in the bacillus subtilis preferably 1 ×
109CFU/g or more, the living bacteria count in the bacillus amyloliquefaciens is 1 × 1010CFU/g or more.The present invention is to described withered
The preparation method of careless bacillus and bacillus amyloliquefaciens is not particularly limited, using the solid fungicide of two kinds of bacterium of conventional preparation
Preparation method.
After inoculation bacillus subtilis and bacillus amyloliquefaciens, ferment the present invention 48 ~ 72h at 28 ~ 32 DEG C, described
The time of fermentation is preferably 56 ~ 60h.The present invention is not particularly limited other methods of the fermentation, using routine.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
After stalk, manioc waste and rice husk powder that mass ratio is 35:10:10 are mixed, the water content for adjusting mixture is 55%, then
Bacillus subtilis and bacillus amyloliquefaciens are inoculated in mixture, ferment 72h at 28 DEG C, obtains fermentation material；
Wherein, total inoculum concentration of bacillus subtilis and bacillus amyloliquefaciens is 15%, bacillus subtilis and solution starch gemma
The mass ratio of bacillus is 1:2, and the living bacteria count in bacillus subtilis is 1 × 109CFU/g or more, bacillus amyloliquefaciens
In living bacteria count 1 × 1010CFU/g or more.
Embodiment 2
After stalk, manioc waste and rice husk powder that mass ratio is 30:5:5 are mixed, the water content for adjusting mixture is 50%, then will
Bacillus subtilis and bacillus amyloliquefaciens are inoculated in mixture, and ferment 60h at 32 DEG C, obtain fermentation material；
Wherein, total inoculum concentration of bacillus subtilis and bacillus amyloliquefaciens is 12%, bacillus subtilis and solution starch gemma
The mass ratio of bacillus is 1:3, and the living bacteria count in bacillus subtilis is 1 × 109CFU/g or more, bacillus amyloliquefaciens
In living bacteria count 1 × 1010CFU/g or more.
Embodiment 3
After stalk, manioc waste and rice husk powder that mass ratio is 40:15:15 are mixed, the water content for adjusting mixture is 60%, then
Bacillus subtilis and bacillus amyloliquefaciens are inoculated in mixture, ferment 48h at 32 DEG C, obtains fermentation material；
Wherein, total inoculum concentration of bacillus subtilis and bacillus amyloliquefaciens is 10%, bacillus subtilis and solution starch gemma
The mass ratio of bacillus is 1:2, and the living bacteria count in bacillus subtilis is 1 × 109CFU/g or more, bacillus amyloliquefaciens
In living bacteria count 1 × 1010CFU/g or more.
Embodiment 4
The fermentation material obtained to embodiment 1 ~ 3 carries out thick protein and coarse-fibred analysis, wherein thick protein and coarse-fibred
Measurement is carried out according to the standard of GB/T6432-1994 and GB/T6434-1994, the results are shown in Table 1.
1 thick protein of table and coarse-fibred content
| Thick protein % | Crude fibre % | |
| Embodiment 1 | 25.36 | 26.32 |
| Embodiment 2 | 24.85 | 25.68 |
| Embodiment 3 | 26.34 | 27.48 |
According to table 1, using fermentation process of the invention, in obtained fermentation material the content of crude protein be 24.85 ~
26.34%, coarse-fibred content is 25.68 ~ 27.48%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of fermentation process of stalk, which comprises the following steps:
After stalk, manioc waste, rice husk powder are mixed, the water content for adjusting mixture is 50 ~ 60%, then by bacillus subtilis and
Bacillus amyloliquefaciens are inoculated in mixture, and ferment 48 ~ 72h at 28 ~ 32 DEG C.
2. fermentation process according to claim 1, which is characterized in that the stalk includes corn stover and/or Wheat Straw
Stalk.
3. fermentation process according to claim 1, which is characterized in that the stalk crossed after being mechanically pulverized after 20 meshes again with
Manioc waste, rice husk powder mixing.
4. fermentation process according to claim 1, which is characterized in that the weight ratio of the stalk, manioc waste and rice husk powder
For (30 ~ 40): (5 ~ 15): (5 ~ 15).
5. fermentation process according to claim 4, which is characterized in that the weight ratio of the stalk, manioc waste and rice husk powder
For 35:10:10.
6. fermentation process according to claim 1, which is characterized in that the water content of the mixture is 55%.
7. fermentation process according to claim 1, which is characterized in that the bacillus subtilis and bacillus amyloliquefaciens
Total inoculum concentration be 10 ~ 15%.
8. fermentation process according to claim 7, which is characterized in that the bacillus subtilis and bacillus amyloliquefaciens
Mass ratio be (1 ~ 3): (2 ~ 6).
9. fermentation process according to claim 7 or 8, which is characterized in that effective viable bacteria in the bacillus subtilis
Number is 1 × 109CFU/g or more.
10. fermentation process according to claim 7 or 8, which is characterized in that effective work in the bacillus amyloliquefaciens
Bacterium number is 1 × 1010CFU/g or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910842097.3A CN110432386A (en) | 2019-09-06 | 2019-09-06 | A kind of fermentation process of stalk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910842097.3A CN110432386A (en) | 2019-09-06 | 2019-09-06 | A kind of fermentation process of stalk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110432386A true CN110432386A (en) | 2019-11-12 |
Family
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|---|---|---|---|
| CN201910842097.3A Pending CN110432386A (en) | 2019-09-06 | 2019-09-06 | A kind of fermentation process of stalk |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855744A (en) * | 2015-06-25 | 2015-08-26 | 孙笑雨 | Straw feed |
| US20150290254A1 (en) * | 2013-12-31 | 2015-10-15 | Dupont Nutrition Biosciences Aps | Novel blends of bacillus strains and enzymes |
| CN106689669A (en) * | 2017-01-11 | 2017-05-24 | 黑龙江省兽医科学研究所 | Corn straw biological composite leavening agent and method for producing silage corn feed by using same |
| CN109287855A (en) * | 2018-09-28 | 2019-02-01 | 青岛农业大学 | A kind of fermentation process of corn stover, the fermented maize stalk of this method preparation and application |
-
2019
- 2019-09-06 CN CN201910842097.3A patent/CN110432386A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150290254A1 (en) * | 2013-12-31 | 2015-10-15 | Dupont Nutrition Biosciences Aps | Novel blends of bacillus strains and enzymes |
| CN104855744A (en) * | 2015-06-25 | 2015-08-26 | 孙笑雨 | Straw feed |
| CN106689669A (en) * | 2017-01-11 | 2017-05-24 | 黑龙江省兽医科学研究所 | Corn straw biological composite leavening agent and method for producing silage corn feed by using same |
| CN109287855A (en) * | 2018-09-28 | 2019-02-01 | 青岛农业大学 | A kind of fermentation process of corn stover, the fermented maize stalk of this method preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| 于向春等: "发酵木薯渣粉在文昌鸡日粮中的应用", 《中国农学通报》 * |
| 戴圻霏等: "解淀粉芽孢杆菌产纤维素酶及其酶解能力相关研究进展", 《安徽农业科学》 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191112 |
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