CN106689669A - Corn straw biological composite leavening agent and method for producing silage corn feed by using same - Google Patents
Corn straw biological composite leavening agent and method for producing silage corn feed by using same Download PDFInfo
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- CN106689669A CN106689669A CN201710017317.XA CN201710017317A CN106689669A CN 106689669 A CN106689669 A CN 106689669A CN 201710017317 A CN201710017317 A CN 201710017317A CN 106689669 A CN106689669 A CN 106689669A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention provides a corn straw biological composite leavening agent and a method for producing silage corn feed by using the same, and relates to a biological composite leavening agent and a method for producing silage corn feed by using the same. The problems about how to promote corn straw fiber, which is difficult to decompose, to degrade, shorten the fermentation period, improve the nutritional value and the palatability of the corn straw and promote feeding development and utilization of the corn straw are solved. The method for producing the silage corn feed comprises the steps: 1, preparing corn straw bottom materials; 2, preparing a corn straw biological composite leavening agent solution; 3, performing leavening agent inoculation; 4, performing corn straw fermentation. The invention provides the corn straw biological composite leavening agent and the method for producing the silage corn feed by using the same.
Description
Technical field
The present invention relates to biological composite fermentation agent and its method for production corn feed.
Background technology
China is one of maximum country of crop straws for producing amount in the world, wherein, maize straw resource amount is maximum, makees
It is a kind of important biomolecule matter resource, develops maize straw, becomes the roughage resource of ruminant high-quality, it is real
Considerable economic benefit and ecological benefits is showed.However, maize straw main component is cellulose, hemicellulose and lignin,
Molecular structure crystallinity is high with the degree of polymerization, it is difficult to be degraded, fermentation period is long.
The content of the invention
The invention solves the problems that how to promote the corn stalk fiber of difficult decomposition to degrade, shorten fermentation period, improve corn stalk
Stalk nutritive value and palatability, the problem for promoting corn straw feedstuff to develop and use, and a kind of cornstalk biological is provided and is answered
The method for closing leavening and its production silage corn feed.
A kind of cornstalk biological composite ferment is made up of composite bacteria agent and complex enzyme formulation；
Described composite bacteria agent is made up of bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri bacterium powder；
Described complex enzyme formulation is made up of cellulase and zytase；
Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:(1~2)；Described solution starch
Bacillus bacterium powder is 2 with the mass ratio of Lactobacillus reuteri bacterium powder:(1~2)；Described bacillus amyloliquefaciens bacterium powder and fiber
The mass ratio of plain enzyme is 2:(1~1.5)；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of zytase:(1~
1.5)。
A kind of method of cornstalk biological composite ferment production silage corn feed is followed the steps below：
First, maize straw bed material is prepared：
It is 2.5cm ~ 5cm that fresh corn straw utilization pulverizer is blocked to length, and it is 70% ~ 75% to control water content, is obtained
Maize straw bed material；
2nd, cornstalk biological composite fermentation agent solution is configured：
Cornstalk biological composite ferment is dissolved in the physiological saline that mass percent is 0.85% ~ 0.9%, corn stalk is obtained
Stalk biological composite fermentation agent solution；The quality of described cornstalk biological composite ferment and mass percent be 0.85% ~
The volume ratio of 0.9% physiological saline is (50 ~ 100) g:500mL；
Described cornstalk biological composite ferment is made up of composite bacteria agent and complex enzyme formulation；
Described composite bacteria agent is made up of bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri bacterium powder；
Described complex enzyme formulation is made up of cellulase and zytase；
Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:(1~2)；Described solution starch
Bacillus bacterium powder is 2 with the mass ratio of Lactobacillus reuteri bacterium powder:(1~2)；Described bacillus amyloliquefaciens bacterium powder and fiber
The mass ratio of plain enzyme is 2:(1~1.5)；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of zytase:(1~
1.5)；
3rd, fermenting agent inoculation：
By maize straw bed material layer on cement fermentation vat or ground, the thick maize straw bottoms of 25cm ~ 35cm are often piled up
Material just uniformly sprays a cornstalk biological composite fermentation agent solution and is compacted, and last plastic sheeting binds, firming sealing；
Described maize straw bed material and cornstalk biological composite ferment in cornstalk biological composite fermentation agent solution
Mass ratio is 1t:(50~100)g；
4th, maize straw fermentation：
Autumn or temperature complete the compound hair of cornstalk biological under conditions of 20 DEG C ~ 30 DEG C, to be taken after fermentation 20d ~ 35d
The method that ferment agent produces silage corn feed.
The beneficial effects of the invention are as follows：Corn stalk fiber can be degraded to widow by complex enzyme formulation of the present invention rapidly
Sugar and the pool of fiber two, are utilized beneficial to the beneficial bacterial metabolic in composite bacteria agent, and profitable strain is formed in promoting fermented stalk microenvironment,
All kinds of digestive ferments that beneficial bacterial metabolic is produced, further promote corn stalk fiber degraded.
Composite bacteria agent of the present invention, on the one hand, aerobic bacillus amyloliquefaciens and the metabolizable product of bacillus subtilis
Life is various to suppress disease fungus and bacterial components, and effectively prevention ensilage goes mouldy or pathogenetic bacteria pollution.On the other hand, it is aerobic
Bacterium exhausts oxygen in bed material, is that the breeding of anaerobism Lactobacillus reuteri creates advantage so that Bacillus acidi lactici is set up rapidly excellent
Gesture flora status, forms sour environment, promotes maize straw fermentation and curing.
Present invention facilitates corn stalk fiber degraded, ensilage crude fiber content is reduced, shorten stalk fermentation
In the cycle, improve maize straw nutritive value and palatability.
Composite bacteria agent of the present invention and complex enzyme formulation are respectively provided with prebiotic effect, safety, have no toxic side effect, Bu Huizao
Into ecological environmental pollutions such as water body, soil and air, beneficial to the sustainable development of agricultural.
The present invention is for a kind of cornstalk biological composite ferment and its method for production silage corn feed.
Specific embodiment
Specific embodiment one：A kind of cornstalk biological composite ferment of present embodiment is by composite bacteria agent and answers
Synthase preparation is constituted；
Described composite bacteria agent is made up of bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri bacterium powder；
Described complex enzyme formulation is made up of cellulase and zytase；
Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:(1~2)；Described solution starch
Bacillus bacterium powder is 2 with the mass ratio of Lactobacillus reuteri bacterium powder:(1~2)；Described bacillus amyloliquefaciens bacterium powder and fiber
The mass ratio of plain enzyme is 2:(1~1.5)；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of zytase:(1~
1.5)。
The beneficial effect of present embodiment is：Complex enzyme formulation of the present embodiment can rapidly by corn stalk fiber
Oligosaccharides and the pool of fiber two are degraded to, are utilized beneficial to the beneficial bacterial metabolic in composite bacteria agent, it is beneficial in promotion fermented stalk microenvironment
Flora is formed, all kinds of digestive ferments that beneficial bacterial metabolic is produced, and further promotes corn stalk fiber degraded.
Composite bacteria agent of the present embodiment, on the one hand, aerobic bacillus amyloliquefaciens and bacillus subtilis can generations
Thank to various suppression disease fungus and the bacterial components of generation, effectively prevention ensilage goes mouldy or pathogenetic bacteria pollution.On the other hand,
Aerobic bacteria exhausts oxygen in bed material, is that the breeding of anaerobism Lactobacillus reuteri creates advantage so that Bacillus acidi lactici is built rapidly
Vertical dominant microflora status, forms sour environment, promotes maize straw fermentation and curing.
Present embodiment promotes corn stalk fiber degraded, reduces ensilage crude fiber content, shortens stalk
Fermentation period, improves maize straw nutritive value and palatability.
Composite bacteria agent of the present embodiment and complex enzyme formulation are respectively provided with prebiotic effect, safety, have no toxic side effect, no
The ecological environmental pollutions such as water body, soil and air can be caused, beneficial to the sustainable development of agricultural.
Specific embodiment two：Present embodiment from unlike specific embodiment one：Described solution starch gemma bar
Bacterium bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:1；Described bacillus amyloliquefaciens bacterium powder and Lactobacillus reuteri bacterium
The mass ratio of powder is 2:1；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of cellulase:1；Described solution starch
Bacillus bacterium powder is 2 with the mass ratio of zytase:1.Other are identical with specific embodiment one.
Specific embodiment three：Unlike one of present embodiment and specific embodiment one or two：The solution starch
Bacillus is bacillus amyloliquefaciens SSYB；The bacillus subtilis is bacillus subtilis SY6；The Lactobacillus reuteri
It is Lactobacillus reuteri DYF5.Other are identical with specific embodiment one or two.
Specific embodiment four：Unlike one of present embodiment and specific embodiment one to three：Described is compound
Microbial inoculum is prepared according to the following steps：
Bacillus amyloliquefaciens, bacillus subtilis and Lactobacillus reuteri are carried out into liquid state fermentation respectively, thalline is collected by centrifugation, so
Prepare and collect using spray drying and Freeze Drying Technique afterwards, respectively obtain bacillus amyloliquefaciens bacterium powder, bacillus subtilis
Bacterium bacterium powder and Lactobacillus reuteri bacterium powder, then by bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri
Bacterium powder mixes, and obtains composite bacteria agent；
Viable count is more than 10 in described bacillus amyloliquefaciens bacterium powder8CFU/g；Viable bacteria in described bacillus subtilis bacterium powder
Number is more than 108CFU/g；Viable count is more than 10 in described Lactobacillus reuteri bacterium powder8CFU/g.Other with specific embodiment one to
Three is identical.
Specific embodiment five：Unlike one of present embodiment and specific embodiment one to four：Described fiber
Plain enzyme enzyme activity is 500U/g ~ 1500U/g；Described xylanase activity power is 1000U/g ~ 2000U/g.Other and specific reality
Apply mode one to four identical.
Specific embodiment six：A kind of cornstalk biological composite ferment production silage corn described in present embodiment
The method of feed is followed the steps below：
First, maize straw bed material is prepared：
It is 2.5cm ~ 5cm that fresh corn straw utilization pulverizer is blocked to length, and it is 70% ~ 75% to control water content, is obtained
Maize straw bed material；
2nd, cornstalk biological composite fermentation agent solution is configured：
Cornstalk biological composite ferment is dissolved in the physiological saline that mass percent is 0.85% ~ 0.9%, corn stalk is obtained
Stalk biological composite fermentation agent solution；The quality of described cornstalk biological composite ferment and mass percent be 0.85% ~
The volume ratio of 0.9% physiological saline is (50 ~ 100) g:500mL；
Described cornstalk biological composite ferment is made up of composite bacteria agent and complex enzyme formulation；
Described composite bacteria agent is made up of bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri bacterium powder；
Described complex enzyme formulation is made up of cellulase and zytase；
Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:(1~2)；Described solution starch
Bacillus bacterium powder is 2 with the mass ratio of Lactobacillus reuteri bacterium powder:(1~2)；Described bacillus amyloliquefaciens bacterium powder and fiber
The mass ratio of plain enzyme is 2:(1~1.5)；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of zytase:(1~
1.5)；
3rd, fermenting agent inoculation：
By maize straw bed material layer on cement fermentation vat or ground, the thick maize straw bottoms of 25cm ~ 35cm are often piled up
Material just uniformly sprays a cornstalk biological composite fermentation agent solution and is compacted, and last plastic sheeting binds, firming sealing；
Described maize straw bed material and cornstalk biological composite ferment in cornstalk biological composite fermentation agent solution
Mass ratio is 1t:(50~100)g；
4th, maize straw fermentation：
Autumn or temperature complete the compound hair of cornstalk biological under conditions of 20 DEG C ~ 30 DEG C, to be taken after fermentation 20d ~ 35d
The method that ferment agent produces silage corn feed.
The beneficial effect of present embodiment is：Complex enzyme formulation of the present embodiment can rapidly by corn stalk fiber
Oligosaccharides and the pool of fiber two are degraded to, are utilized beneficial to the beneficial bacterial metabolic in composite bacteria agent, it is beneficial in promotion fermented stalk microenvironment
Flora is formed, all kinds of digestive ferments that beneficial bacterial metabolic is produced, and further promotes corn stalk fiber degraded.
Composite bacteria agent of the present embodiment, on the one hand, aerobic bacillus amyloliquefaciens and bacillus subtilis can generations
Thank to various suppression disease fungus and the bacterial components of generation, effectively prevention ensilage goes mouldy or pathogenetic bacteria pollution.On the other hand,
Aerobic bacteria exhausts oxygen in bed material, is that the breeding of anaerobism Lactobacillus reuteri creates advantage so that Bacillus acidi lactici is built rapidly
Vertical dominant microflora status, forms sour environment, promotes maize straw fermentation and curing.
Present embodiment promotes corn stalk fiber degraded, reduces ensilage crude fiber content, shortens stalk
Fermentation period, improves maize straw nutritive value and palatability.
Composite bacteria agent of the present embodiment and complex enzyme formulation are respectively provided with prebiotic effect, safety, have no toxic side effect, no
The ecological environmental pollutions such as water body, soil and air can be caused, beneficial to the sustainable development of agricultural.
Specific embodiment seven：Present embodiment from unlike specific embodiment six：Xie Dian described in step 2
Afnyloliquefaciens bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:1；Bacillus amyloliquefaciens bacterium described in step 2
Powder is 2 with the mass ratio of Lactobacillus reuteri bacterium powder:1；Bacillus amyloliquefaciens bacterium powder and cellulase described in step 2
Mass ratio is 2:1；Bacillus amyloliquefaciens bacterium powder and the mass ratio of zytase described in step 2 are 2:1.Other and tool
Body implementation method six is identical.
Specific embodiment eight：Unlike one of present embodiment and specific embodiment six or seven：Institute in step 2
Bacillus amyloliquefaciens are stated for bacillus amyloliquefaciens SSYB；Bacillus subtilis described in step 2 is bacillus subtilis
SY6；Lactobacillus reuteri described in step 2 is Lactobacillus reuteri DYF5.Other are identical with specific embodiment six or seven.
Specific embodiment nine：Unlike one of present embodiment and specific embodiment six to eight：Institute in step 2
The composite bacteria agent stated is prepared according to the following steps：
Bacillus amyloliquefaciens, bacillus subtilis and Lactobacillus reuteri are carried out into liquid state fermentation respectively, thalline is collected by centrifugation, so
Prepare and collect using spray drying and Freeze Drying Technique afterwards, respectively obtain bacillus amyloliquefaciens bacterium powder, bacillus subtilis
Bacterium bacterium powder and Lactobacillus reuteri bacterium powder, then by bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri
Bacterium powder mixes, and obtains composite bacteria agent；
Viable count is more than 10 in described bacillus amyloliquefaciens bacterium powder8CFU/g；Viable bacteria in described bacillus subtilis bacterium powder
Number is more than 108CFU/g；Viable count is more than 10 in described Lactobacillus reuteri bacterium powder8CFU/g.Other with specific embodiment six to
Eight is identical.
Specific embodiment ten：Unlike one of present embodiment and specific embodiment six to nine：Institute in step 2
The cellulose enzyme vigor stated is 500U/g ~ 1500U/g；Xylanase activity power described in step 2 for 1000U/g ~
2000U/g.Other are identical with specific embodiment six to nine.
Beneficial effects of the present invention are verified using following examples：
Embodiment one：
A kind of method of the cornstalk biological composite ferment production silage corn feed described in the present embodiment is according to following
What step was carried out：
First, maize straw bed material is prepared：
It is 2.5cm that fresh corn straw utilization pulverizer is blocked to length, and it is 70% to control water content, obtains maize straw
Bed material；
2nd, cornstalk biological composite fermentation agent solution is configured：
60g cornstalk biological composite ferments are dissolved in the physiological saline that 500mL mass percents are 0.85%, jade is obtained
Rice straw biological composite fermentation agent solution；
Described cornstalk biological composite ferment is made up of composite bacteria agent and complex enzyme formulation；
Described composite bacteria agent is by 20g bacillus amyloliquefaciens bacterium powder, 10g bacillus subtilises bacterium powder and 10g Lactobacillus reuteris
Bacterium powder is constituted；
Described complex enzyme formulation is made up of 10g cellulases and 10g zytases；
3rd, fermenting agent inoculation：
By maize straw bed material layer on cement fermentation vat or ground, the thick maize straw bed materials of 25cm are often piled up just equal
Cornstalk biological composite fermentation agent solution of even sprinkling is simultaneously compacted, and last plastic sheeting binds, firming sealing；
Described maize straw bed material and cornstalk biological composite ferment in cornstalk biological composite fermentation agent solution
Mass ratio is 1t:60g；
4th, maize straw fermentation：
Under conditions of being 20 DEG C in temperature, taken after fermentation 35d, obtain cornstalk silage, that is, complete maize straw life
The method that thing composite ferment produces silage corn feed.
Composite bacteria agent described in step 2 is prepared according to the following steps：
Bacillus amyloliquefaciens, bacillus subtilis and Lactobacillus reuteri are carried out into liquid state fermentation respectively, thalline is collected by centrifugation, so
Prepare and collect using spray drying and Freeze Drying Technique afterwards, respectively obtain bacillus amyloliquefaciens bacterium powder, bacillus subtilis
Bacterium bacterium powder and Lactobacillus reuteri bacterium powder, then by bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri
Bacterium powder mixes, and obtains composite bacteria agent；
Viable count is more than 10 in described bacillus amyloliquefaciens bacterium powder8CFU/g；Viable bacteria in described bacillus subtilis bacterium powder
Number is more than 108CFU/g；Viable count is more than 10 in described Lactobacillus reuteri bacterium powder8CFU/g。
Described bacillus amyloliquefaciens are SSYB plants；Described bacillus subtilis is SY6 plants；Described Roy's breast bar
Bacterium is DYF5 plants；
Described bacillus amyloliquefaciens are disclosed in for SSYB plants《Chinese veterinary science》, 2016,46（10）：Page 1328 ~ 1334
What page was delivered《The research of bacillus amyloliquefaciens solid state fermentation Radix Astragali culture medium》；
Described bacillus subtilis is disclosed in for SY6 plants《Chinese biological combinations magazine》, 01 month 2013 volume 26 the 1st
Phase, page 122 ~ page 128 deliver《The optimization of pig source bacillus subtilis drying process with atomizing》；
Described Lactobacillus reuteri is disclosed in for DYF5 plants《Herding and animal doctor》What page 76 ~ page 80 of 9 phase of volume 47 in 2015 delivered《Sieve
Her lactobacillus drying process with atomizing condition optimizing》.
Cellulose enzyme vigor described in step 2 is 500U/g；Described xylanase activity power is 1000U/g.
Crude protein content brings up to 10.6%, acidity by 6.3% in cornstalk silage finished product manufactured in the present embodiment
Washing fiber content is reduced to 16.2% by 27.5%.Additionally, be rich in various probiotics bacterial body proteins, digestive ferment and growth promotion because
Son, the nutritive value and ruminant that improve cornstalk silage is digested and assimilated to nutriment in ensilage
Rate.
Cornstalk silage finished product quality manufactured in the present embodiment is soft, and color and luster is in green or yellow green, with light
Micro- tart flavour and wine flavour, lactic acid content 4.1 %, pH 3.6, water content 69%, with good palatability, improves ruminant
Feed intake.
The present embodiment can accelerate fermentation reaction process, the cornstalk silage fermenting and producing time is shortened 9 days.
The various probiotics being rich in the present embodiment cornstalk biological composite ferment can ferment and produce various restraining epiphytes
Material, the restraining epiphyte ability with wide spectrum, cornstalk silage finished product Kaifeng can keep 10 days nothings to go mouldy after taking out.
Embodiment two：
A kind of method of the cornstalk biological composite ferment production silage corn feed described in the present embodiment is according to following
What step was carried out：
First, maize straw bed material is prepared：
It is 3cm that fresh corn straw utilization pulverizer is blocked to length, and it is 72% to control water content, obtains maize straw bottom
Material；
2nd, cornstalk biological composite fermentation agent solution is configured：
78g cornstalk biological composite ferments are dissolved in the physiological saline that 500mL mass percents are 0.86%, jade is obtained
Rice straw biological composite fermentation agent solution；
Described cornstalk biological composite ferment is made up of composite bacteria agent and complex enzyme formulation；
Described composite bacteria agent is by 26g bacillus amyloliquefaciens bacterium powder, 13g bacillus subtilises bacterium powder and 13g Lactobacillus reuteris
Bacterium powder is constituted；
Described complex enzyme formulation is made up of 13g cellulases and 13g zytases；
3rd, fermenting agent inoculation：
By maize straw bed material layer on cement fermentation vat or ground, the thick maize straw bed materials of 28cm are often piled up just equal
Cornstalk biological composite fermentation agent solution of even sprinkling is simultaneously compacted, and last plastic sheeting binds, firming sealing；
Described maize straw bed material and cornstalk biological composite ferment in cornstalk biological composite fermentation agent solution
Mass ratio is 1t:78g；
4th, maize straw fermentation：
Under conditions of being 23 DEG C in temperature, taken after fermentation 30d, obtain cornstalk silage, that is, complete maize straw life
The method that thing composite ferment produces silage corn feed.
Composite bacteria agent described in step 2 is prepared according to the following steps：
Bacillus amyloliquefaciens, bacillus subtilis and Lactobacillus reuteri are carried out into liquid state fermentation respectively, thalline is collected by centrifugation, so
Prepare and collect using spray drying and Freeze Drying Technique afterwards, respectively obtain bacillus amyloliquefaciens bacterium powder, bacillus subtilis
Bacterium bacterium powder and Lactobacillus reuteri bacterium powder, then by bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri
Bacterium powder mixes, and obtains composite bacteria agent；
Viable count is more than 10 in described bacillus amyloliquefaciens bacterium powder8CFU/g；Viable bacteria in described bacillus subtilis bacterium powder
Number is more than 108CFU/g；Viable count is more than 10 in described Lactobacillus reuteri bacterium powder8CFU/g；
Described bacillus amyloliquefaciens are SSYB plants；Described bacillus subtilis is SY6 plants；Described Lactobacillus reuteri is
DYF5 plants；
Described bacillus amyloliquefaciens are disclosed in for SSYB plants《Chinese veterinary science》, 2016,46（10）：Page 1328 ~ 1334
What page was delivered《The research of bacillus amyloliquefaciens solid state fermentation Radix Astragali culture medium》；
Described bacillus subtilis is disclosed in for SY6 plants《Chinese biological combinations magazine》, 01 month 2013 volume 26 the 1st
Phase, page 122 ~ page 128 deliver《The optimization of pig source bacillus subtilis drying process with atomizing》；
Described Lactobacillus reuteri is disclosed in for DYF5 plants《Herding and animal doctor》What page 76 ~ page 80 of 9 phase of volume 47 in 2015 delivered《Sieve
Her lactobacillus drying process with atomizing condition optimizing》.
Cellulose enzyme vigor described in step 2 is 1000U/g；Described xylanase activity power is 1500U/g.
Crude protein content brings up to 10.2%, acidity by 7.7% in cornstalk silage finished product manufactured in the present embodiment
Washing fiber content is reduced to 19.7% by 30.2%.Additionally, be rich in various probiotics bacterial body proteins, digestive ferment and growth promotion because
Son, the nutritive value and ruminant that improve cornstalk silage is digested and assimilated to nutriment in ensilage
Rate.
Cornstalk silage finished product quality manufactured in the present embodiment is soft, and color and luster is in green or yellow green, with light
Micro- tart flavour and wine flavour, lactic acid content 3.9 %, pH 3.8, water content 70%, with good palatability, improves ruminant
Feed intake.
The present embodiment can accelerate fermentation reaction process, the cornstalk silage fermenting and producing time is shortened 7 days.
The various probiotics being rich in the present embodiment cornstalk biological composite ferment can ferment and produce various restraining epiphytes
Material, the restraining epiphyte ability with wide spectrum, cornstalk silage finished product Kaifeng can keep 8 days nothings to go mouldy after taking out.
Embodiment three：
A kind of method of the cornstalk biological composite ferment production silage corn feed described in the present embodiment is according to following
What step was carried out：
First, maize straw bed material is prepared：
It is 3.3cm that fresh corn straw utilization pulverizer is blocked to length, and it is 75% to control water content, obtains maize straw
Bed material；
2nd, cornstalk biological composite fermentation agent solution is configured：
90g cornstalk biological composite ferments are dissolved in the physiological saline that 500mL mass percents are 0.9%, corn is obtained
Straw biological composite fermentation agent solution；
Described cornstalk biological composite ferment is made up of composite bacteria agent and complex enzyme formulation；
Described composite bacteria agent is by 30g bacillus amyloliquefaciens bacterium powder, 15g bacillus subtilises bacterium powder and 15g Lactobacillus reuteris
Bacterium powder is constituted；
Described complex enzyme formulation is made up of 15g cellulases and 15g zytases；
3rd, fermenting agent inoculation：
By maize straw bed material layer on cement fermentation vat or ground, the thick maize straw bed materials of 30cm are often piled up just equal
Cornstalk biological composite fermentation agent solution of even sprinkling is simultaneously compacted, and last plastic sheeting binds, firming sealing；
Described maize straw bed material and cornstalk biological composite ferment in cornstalk biological composite fermentation agent solution
Mass ratio is 1t:90g；
4th, maize straw fermentation：
Under conditions of being 25 DEG C in temperature, taken after fermentation 25d, obtain cornstalk silage, that is, complete maize straw life
The method that thing composite ferment produces silage corn feed.
Composite bacteria agent described in step 2 is prepared according to the following steps：
Bacillus amyloliquefaciens, bacillus subtilis and Lactobacillus reuteri are carried out into liquid state fermentation respectively, thalline is collected by centrifugation, so
Prepare and collect using spray drying and Freeze Drying Technique afterwards, respectively obtain bacillus amyloliquefaciens bacterium powder, bacillus subtilis
Bacterium bacterium powder and Lactobacillus reuteri bacterium powder, then by bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri
Bacterium powder mixes, and obtains composite bacteria agent；
Viable count is more than 10 in described bacillus amyloliquefaciens bacterium powder8CFU/g；Viable bacteria in described bacillus subtilis bacterium powder
Number is more than 108CFU/g；Viable count is more than 10 in described Lactobacillus reuteri bacterium powder8CFU/g。
Described bacillus amyloliquefaciens are SSYB plants；Described bacillus subtilis is SY6 plants；Described Roy's breast bar
Bacterium is DYF5 plants；
Described bacillus amyloliquefaciens are disclosed in for SSYB plants《Chinese veterinary science》, 2016,46（10）：Page 1328 ~ 1334
What page was delivered《The research of bacillus amyloliquefaciens solid state fermentation Radix Astragali culture medium》；
Described bacillus subtilis is disclosed in for SY6 plants《Chinese biological combinations magazine》, 01 month 2013 volume 26 the 1st
Phase, page 122 ~ page 128 deliver《The optimization of pig source bacillus subtilis drying process with atomizing》；
Described Lactobacillus reuteri is disclosed in for DYF5 plants《Herding and animal doctor》What page 76 ~ page 80 of 9 phase of volume 47 in 2015 delivered《Sieve
Her lactobacillus drying process with atomizing condition optimizing》.
Cellulose enzyme vigor described in step 2 is 1000U/g；Described xylanase activity power is 1500U/g.
Crude protein content brings up to 9.3% by 6.1% in cornstalk silage finished product manufactured in the present embodiment, and acidity is washed
Wash fiber content and be reduced to 22.5% by 31.1%.Additionally, various probiotics bacterial body proteins, digestive ferment and somatomedin are rich in,
Improve cornstalk silage nutritive value and ruminant to the digestibility of nutriment in ensilage.
Cornstalk silage finished product quality manufactured in the present embodiment is soft, and color and luster is in green or yellow green, with light
Micro- tart flavour and wine flavour, lactic acid content 3.4 %, PH 3.9, water content 71%, with good palatability, improves ruminant
Feed intake.
The present embodiment can accelerate fermentation reaction process, the cornstalk silage fermenting and producing time is shortened 7 days.
The various probiotics being rich in the present embodiment cornstalk biological composite ferment can ferment and produce various restraining epiphytes
Material, the restraining epiphyte ability with wide spectrum, cornstalk silage finished product Kaifeng can keep 7 days nothings to go mouldy after taking out.
Claims (10)
1. a kind of cornstalk biological composite ferment, it is characterised in that a kind of cornstalk biological composite ferment is by being combined
Microbial inoculum and complex enzyme formulation are constituted；
Described composite bacteria agent is made up of bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri bacterium powder；
Described complex enzyme formulation is made up of cellulase and zytase；
Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:(1~2)；Described solution starch
Bacillus bacterium powder is 2 with the mass ratio of Lactobacillus reuteri bacterium powder:(1~2)；Described bacillus amyloliquefaciens bacterium powder and fiber
The mass ratio of plain enzyme is 2:(1~1.5)；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of zytase:(1~
1.5)。
2. a kind of cornstalk biological composite ferment according to claim 1, it is characterised in that described solution starch bud
Spore bacillus bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:1；Described bacillus amyloliquefaciens bacterium powder and Roy's breast bar
The mass ratio of bacterium bacterium powder is 2:1；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of cellulase:1；Described solution
Bacillus amyloliquefacienses bacterium powder is 2 with the mass ratio of zytase:1.
3. a kind of cornstalk biological composite ferment according to claim 1, it is characterised in that the solution starch gemma
Bacillus is bacillus amyloliquefaciens SSYB；The bacillus subtilis is bacillus subtilis SY6；The Lactobacillus reuteri is sieve
She is lactobacillus DYF5.
4. a kind of cornstalk biological composite ferment according to claim 1, it is characterised in that described composite bacteria agent
Prepare according to the following steps：
Bacillus amyloliquefaciens, bacillus subtilis and Lactobacillus reuteri are carried out into liquid state fermentation respectively, thalline is collected by centrifugation, so
Prepare and collect using spray drying and Freeze Drying Technique afterwards, respectively obtain bacillus amyloliquefaciens bacterium powder, bacillus subtilis
Bacterium bacterium powder and Lactobacillus reuteri bacterium powder, then by bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri
Bacterium powder mixes, and obtains composite bacteria agent；
Viable count is more than 10 in described bacillus amyloliquefaciens bacterium powder8CFU/g；Viable bacteria in described bacillus subtilis bacterium powder
Number is more than 108CFU/g；Viable count is more than 10 in described Lactobacillus reuteri bacterium powder8CFU/g。
5. a kind of cornstalk biological composite ferment according to claim 1, it is characterised in that described cellulase
Enzyme activity is 500U/g ~ 1500U/g；Described xylanase activity power is 1000U/g ~ 2000U/g.
6. a kind of a kind of method that cornstalk biological composite ferment produces silage corn feed, it is characterised in that maize straw
The method of biological composite fermentation agent production silage corn feed is followed the steps below：
First, maize straw bed material is prepared：
It is 2.5cm ~ 5cm that fresh corn straw utilization pulverizer is blocked to length, and it is 70% ~ 75% to control water content, is obtained
Maize straw bed material；
2nd, cornstalk biological composite fermentation agent solution is configured：
Cornstalk biological composite ferment is dissolved in the physiological saline that mass percent is 0.85% ~ 0.9%, corn stalk is obtained
Stalk biological composite fermentation agent solution；The quality of described cornstalk biological composite ferment and mass percent be 0.85% ~
The volume ratio of 0.9% physiological saline is (50 ~ 100) g:500mL；
Described cornstalk biological composite ferment is made up of composite bacteria agent and complex enzyme formulation；
Described composite bacteria agent is made up of bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri bacterium powder；
Described complex enzyme formulation is made up of cellulase and zytase；
Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of bacillus subtilis bacterium powder:(1~2)；Described solution starch
Bacillus bacterium powder is 2 with the mass ratio of Lactobacillus reuteri bacterium powder:(1~2)；Described bacillus amyloliquefaciens bacterium powder and fiber
The mass ratio of plain enzyme is 2:(1~1.5)；Described bacillus amyloliquefaciens bacterium powder is 2 with the mass ratio of zytase:(1~
1.5)；
3rd, fermenting agent inoculation：
By maize straw bed material layer on cement fermentation vat or ground, the thick maize straw bottoms of 25cm ~ 35cm are often piled up
Material just uniformly sprays a cornstalk biological composite fermentation agent solution and is compacted, and last plastic sheeting binds, firming sealing；
Described maize straw bed material and cornstalk biological composite ferment in cornstalk biological composite fermentation agent solution
Mass ratio is 1t:(50~100)g；
4th, maize straw fermentation：
Autumn or temperature complete the compound hair of cornstalk biological under conditions of 20 DEG C ~ 30 DEG C, to be taken after fermentation 20d ~ 35d
The method that ferment agent produces silage corn feed.
7. the method that a kind of cornstalk biological composite ferment according to claim 6 produces silage corn feed, its
The bacillus amyloliquefaciens bacterium powder and the mass ratio of bacillus subtilis bacterium powder being characterised by described in step 2 are 2:1；Step
Bacillus amyloliquefaciens bacterium powder and the mass ratio of Lactobacillus reuteri bacterium powder described in two are 2:1；Xie Dian described in step 2
Afnyloliquefaciens bacterium powder is 2 with the mass ratio of cellulase:1；Bacillus amyloliquefaciens bacterium powder described in step 2 is gathered with wood
The mass ratio of carbohydrase is 2:1.
8. the method that a kind of cornstalk biological composite ferment according to claim 6 produces silage corn feed, its
Feature bacillus amyloliquefaciens described in step 2 are bacillus amyloliquefaciens SSYB；Bacillus subtilis described in step 2
It is bacillus subtilis SY6；Lactobacillus reuteri described in step 2 is Lactobacillus reuteri DYF5.
9. the method that a kind of cornstalk biological composite ferment according to claim 6 produces silage corn feed, its
It is characterised by that the composite bacteria agent described in step 2 is prepared according to the following steps：
Bacillus amyloliquefaciens, bacillus subtilis and Lactobacillus reuteri are carried out into liquid state fermentation respectively, thalline is collected by centrifugation, so
Prepare and collect using spray drying and Freeze Drying Technique afterwards, respectively obtain bacillus amyloliquefaciens bacterium powder, bacillus subtilis
Bacterium bacterium powder and Lactobacillus reuteri bacterium powder, then by bacillus amyloliquefaciens bacterium powder, bacillus subtilis bacterium powder and Lactobacillus reuteri
Bacterium powder mixes, and obtains composite bacteria agent；
Viable count is more than 10 in described bacillus amyloliquefaciens bacterium powder8CFU/g；Viable bacteria in described bacillus subtilis bacterium powder
Number is more than 108CFU/g；Viable count is more than 10 in described Lactobacillus reuteri bacterium powder8CFU/g。
10. the method that a kind of cornstalk biological composite ferment according to claim 6 produces silage corn feed, its
It is characterised by that the cellulose enzyme vigor described in step 2 is 500U/g ~ 1500U/g；Zytase enzyme described in step 2
Vigor is 1000U/g ~ 2000U/g.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102048025A (en) * | 2010-11-10 | 2011-05-11 | 天津科建科技发展有限公司 | Composite leavening agent combining xylanase with multiple strains and method for fermenting straw feed |
-
2017
- 2017-01-11 CN CN201710017317.XA patent/CN106689669A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102048025A (en) * | 2010-11-10 | 2011-05-11 | 天津科建科技发展有限公司 | Composite leavening agent combining xylanase with multiple strains and method for fermenting straw feed |
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