Disclosure of Invention
The invention aims to provide an effective cultivation method for improving the bud number and the plant type fullness of a single plant of chrysanthemum.
The method for cultivating the chrysanthemum morifolium provided by the invention comprises the following steps: starting to supplement light on the 6 th to 13 th days of short-day treatment; the supplementary lighting is continuously supplemented for 7-9 days, and the method for supplementing lighting every day comprises the following steps: and 4-6 hours are selected for light supplement in the time period from sunset to sunrise of the next day.
In the cultivation method, light supplement is started on the 6 th day, the 7 th day, the 8 th day, the 9 th day, the 10 th day, the 11 th day, the 12 th day or the 13 th day of short-day treatment, and light supplement is continuously performed for 7 days, 8 days or 9 days; the method for supplementing light every day comprises the following steps: the light supplement is carried out for 4 hours, 5 hours or 6 hours randomly in the time period from the sunset to the sunrise of the next day, and the light supplement time can be continuous (such as continuous light supplement for 4 hours, 5 hours or 6 hours) or discontinuous (such as continuous light supplement for 2 hours, then stopping light supplement for 1 hour, and then continuous light supplement for 2 hours).
Further, light supplement is started at the 6 th to 10 th days of short-day treatment, and light supplement is continuously carried out for 7 to 8 days; the method for supplementing light every day comprises the following steps: the light supplement is carried out for 4-5 hours in the time period from 20:00 at night to 4:00 in the next morning (Beijing time). For example, the light is continuously supplemented for 5 hours in the time period from 20:00 at night to 1:00 (Beijing time) in the next morning; or continuously supplementing light for 2 hours from 21:00 at night to 23:00 at night (Beijing time), stopping supplementing light for 2 hours, and continuously supplementing light for 2 hours from 1:00 at early morning to 3:00 at early morning (Beijing time).
Furthermore, light supplement is started on the 6 th day of short-day treatment, and light supplement is continuously carried out for 8 days; the method for supplementing light every day comprises the following steps: continuously supplementing light for 4 hours from 22:00 at night to 2:00 (Beijing time) in the morning of the next day.
In the cultivation method, the light intensity of the supplementary lighting is at least 50 Lux.
Further, the light intensity of the supplementary lighting can be 50-70 Lux.
Furthermore, the light intensity of the supplementary lighting can be 50-60 Lux.
In an embodiment of the present invention, the fill light has a light intensity of 50 Lux.
In the above cultivation method, the starting time of the short-day treatment is 12-13 days earlier than the normal starting time. Taking a growth period (consisting of a vegetative growth period and a reproductive growth period in sequence) of 90 days, taking a small chrysanthemum which can flower after the short-day condition lasts for 45 days as an example, the normal starting time of the short-day treatment is the 46 th day after the beginning of the vegetative growth period; in the method of the present invention, on the other hand, the initiation time of the short-day treatment is 12 to 13 days earlier than the normal initiation time, i.e., the short-day treatment is started on day 32 or 33 after the start of the vegetative growth phase.
In one embodiment of the present invention, the start time of the short-day treatment is 13 days earlier than the normal start time. Taking the example of a growth period (consisting of a vegetative growth period and a reproductive growth period in this order) of 85 days, and a short-day condition for 43 days before flowering, the normal starting time of the short-day treatment is 43 days after the vegetative growth period; in the method of the present invention, on the other hand, the initiation time of the short-day treatment is 13 days earlier than the normal initiation time, i.e., the short-day treatment is started on day 30 after the start of the vegetative growth phase.
In the cultivation method, the short-day treatment is carried out so that the duration of the short-day treatment does not exceed 10 h/day. The method of short-day treatment may be shading.
In one embodiment of the present invention, the short-day treatment is performed so that the duration of day is 10 h/day.
The cultivation method also comprises the following steps: when the branch length of the plant is 1-2cm, the plant is treated by a plant growth inhibitor to control the length of the first section and the second section of the branch, so as to prevent the high position from overgrowing and the branch from overlong.
The cultivation method also comprises the following steps: when the diameter of the main bud of the plant branch is 2-3mm, the plant is treated by a plant growth inhibitor so as to control the length of the flower head of each branch and achieve the commodity export standard.
Further, the plant growth inhibitor can be a plant growth inhibitor commonly used in the prior art, such as paclobutrazol, high-efficiency azole, bicolor and the like.
Further, the plant growth inhibitor may specifically be daminozide.
The method for treating plants with the plant growth inhibitor can be specifically spraying.
In one embodiment of the invention, when the branch length of the plant is 1-2cm, spraying for one time and diluting for 800 times; when the diameter of the main bud of the plant branch is 2-3mm, spraying for once more and diluting for 800 times. The dosage of the 800-time diluted daminozide can be 60Kg per mu (32000 small chrysanthemum plants are cultivated per mu).
The cultivation method aims at the cut chrysanthemum with multiple heads.
Further, the chrysanthemum indicum is of autumn chrysanthemum series, and the whole growth period (sequentially consisting of vegetative growth period and reproductive growth period) can be 85-90 days.
Further, the feverfew may be a feverfew of japan.
In a specific embodiment of the present invention, the variety of the Japanese chrysanthemum may be Hongchang or Tiaodan or Xinhongxiao.
The chrysanthemum morifolium cultivated by the method also belongs to the protection scope of the invention.
The application of the above cultivation method or the chrysanthemum morifolium cultivated according to the above method in any one of the following (1) to (4) also belongs to the protection scope of the invention:
(1) preparing cut chrysanthemum or fresh cut chrysanthemum;
(2) preparing a processed product taking cut-flower chrysanthemum or fresh cut-flower chrysanthemum as a raw material;
(3) preparing Buddha flowers and/or tomb flowers and/or bouquet;
(4) the bud number and/or plant type fullness of the single small chrysanthemum are/is improved.
The light supplement technology is adopted in the method for cultivating the small chrysanthemum, so that the bud number and the plant type fullness of the single small chrysanthemum are improved. The principle of the invention is as follows: when the chrysanthemum enters the reproductive growth period, the flower buds are differentiated one by one from the growth point, and the self-capping of the chrysanthemum plant can be realized by controlling the photoperiod, so that the branches are differentiated to achieve the purpose of improving the bud number and plant type fullness of the single chrysanthemum. The method can produce the cut flower chrysanthemum with good fullness, wherein the length of each branch of the cut flower is more than 70cm, each branch has 3-4 branches, the length of each branch is 10-15cm, at least 5 buds are arranged per branch, the number of buds in 4cm below the top bud of each chrysanthemum is 15-29.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified. The quantitative tests in the following examples, all set up three replicates and the results averaged.
The Japanese chrysanthemum varieties Hongchang and Diandingxindan in the following examples are all products of the Japanese Jingxing garden, and the catalog numbers are 030-.
The daminow Chinese name in the following examples is N-dimethylamino succinamide or butyryl hydrazine, the brand name is Naofeng, and the name is the product of the research institute of dynamic test technology for aerospace in Western's safety. The content of active ingredients is as follows: 92 percent; the preparation formulation is as follows: soluble powder; net content: 50 g.
Example 1 cultivation method of Chrysanthemum parthenium (Red Chang)
A cultivation site: shaoxing city horizontal base in Zhejiang province
Cultivation time: 10 months in 2018-12 months in 2018
First, the cultivation method of the chrysanthemum indicum
1. Obtaining of seedlings of Chrysanthemum indicum
1) Preparation before cutting
Land preparation: and (3) selecting a plot which is loose in soil, high in terrain and not easy to flood, ploughing the plot in one week before cuttage, applying 1 ton of fully-decomposed organic fertilizer per mu during ploughing, and ensuring even ploughing.
Ridging: and (4) making beds according to the standard that the height of the bed is 25cm and the width of the bed is 90 cm.
Making a chrysanthemum net: and laying chrysanthemum nets according to the width of the planting bed. The method comprises the following specific steps: 2 main rods are respectively fixed at two ends of a ridge, one end of the chrysanthemum net is stretched by a cross rod of 80cm and fixed on the two main rods at one end of the ridge, the other end of the chrysanthemum net is stretched by a cross rod of 80cm, the distance from the main rods at the other end of the ridge to the main rods at the other end of the ridge is about 40cm when the chrysanthemum net is naturally spread, and the cross rod is grasped to pull the chrysanthemum net to be sleeved on the main rods at the other end of the ridge.
2) Cutting and seedling raising
Cuttage: collecting branches with the length of about 6-7cm from mother plants of the Japanese chrysanthemum Hongchang (autumn chrysanthemum), and cutting the collected branches in the middle of a chrysanthemum grid, wherein 1 plant is inserted into each grid with the insertion depth of about 2-3 cm. 32000 plants are planted in each mu of the field.
Seedling culture: immediately watering after cuttage, and culturing under the conditions that the air humidity is 90-100%, the environmental temperature is 18-25 ℃, the sunshine time is 14 h/day and the illumination intensity is 2-3 ten thousand Lux for 10-12 days to obtain the chrysanthemum seedling.
2. Cultivation of chrysanthemum indicum plants
And (3) cultivating the small chrysanthemum seedlings obtained in the step (1) into small chrysanthemum plants in the field. The entire growth period for growing the chrysanthemum plantlets into chrysanthemum plants is 85 days. The whole growth period consists of vegetative growth period and reproductive growth period in turn, and the reproductive growth period is started after the short-day treatment is started. The specific cultivation process is as follows:
1) vegetative growth phase
Culturing the young chrysanthemum under the conditions of air humidity of 50-75%, environment temperature of 15-30 ℃, sunshine time of 14 h/day and illumination intensity of 5-10 ten thousand Lux.
2) Growth period of reproduction
2-1) starting time of short day treatment: the initiation time of the short-day treatment was 13 days earlier than the normal initiation time. Since it is possible for Japanese chrysanthemum to bloom by continuing the short-day treatment for 43 days under normal conditions, the short-day treatment is started on day 30 after the start of the vegetative growth period (the start time of the normal short-day treatment is 43 days after the start of the vegetative growth period, and in the present invention, the start time of the short-day treatment is 13 days earlier than the normal start time, so the short-day treatment is started on day 30 after the start of the vegetative growth period).
2-2) short-day treatment: the short-day treatment method is shading, wherein shading is carried out in 17:00-7:00 (next day, Beijing time) every day, the day of the short-day treatment method is 10 h/day, and the purpose is to lead the upper leaves of the plants to sense the photoperiod, thereby turning to the reproductive growth period, leading the growth points of the plants to completely enter the bud scale differentiation period, and differentiating main buds to seal the tops of the plants.
2-3) light supplement: and (3) starting light supplement on the 6 th day (the terminal stage of differentiation of bud scales) of short-day sun treatment, wherein the light supplement time is 22:00 at night to 2:00 in the next morning, the light supplement time is 4 hours, the light supplement time is continuously 8 days, and the light intensity is 50Lux, so that the lateral branches of the plant are differentiated under the condition that the main buds are differentiated and the lateral buds are not differentiated. And after 8 days of continuous light supplement, stopping light supplement, and enabling the plants to sense the photoperiod and fully differentiate.
2-4) plant growth inhibitor treatment: when the branch length of the plant is 1-2cm, spraying 60Kg of diluted 800 times per mu to control the length of the first section and the second section of the branch (control the length of each branch to be about 10cm) so as to prevent overgrowth of high positions and overlong branches.
2-5) plant growth inhibitor treatment: when the diameter of the main bud of each branch is about 2-3mm, spraying 800 times diluted specific time with the amount of 60Kg per mu for controlling the length of the flower head of each branch (the length from the tail end of one uppermost leaf of the chrysanthemum plant to the tail end of the bud) to reach the commodity export standard.
3. Harvesting
And (4) harvesting after the reproductive period is finished.
Second, control group
The cultivation method of the control group is the same as the cultivation method of the invention except that the step of supplementing light in the step one, 2-3) is not provided.
Thirdly, the cultivation effect evaluation of the method and the comparison method
Counting the buds within 4cm below the top buds of the chrysanthemum of each of the control group and the experimental group (taking 30 buds of each group) after the reproductive growth period, and comparing the plant type fullness of the two groups of plants.
The comparison results of the number of buds of the chrysanthemum and the whole plant in the control group and the experimental group are shown in FIG. 1. The number of buds counted is as follows: the number of buds within 4cm below the top buds of each chrysanthemum in the control group is 5-6, and the average number is 5.5; the number of buds within 4cm below the top buds of each chrysanthemum in the experimental group is 15-25, and the average number is 21; the average number of buds within 4cm below the top buds of each chrysanthemum in the experimental group is obviously more than that in the control group. From the plant type of the whole plant: the plant type fullness of the single chrysanthemum of the experimental group is also obviously superior to that of the control group. The method can effectively improve the bud number and the plant type fullness of the single plant of the chrysanthemum.
In addition, the conditions of the branch length, the branch number, the branch length, the branch bud number and the like of each fresh cut flower of the small chrysanthemum in the experimental group are counted. The results show that: the branch length of each fresh cut flower of the small chrysanthemum cultivated by the method is more than 70cm, the number of branches is 3-4, the length of each branch is 10-15cm, and each branch has at least 5 buds.
The results show that the individual plant of the chrysanthemum obtained by the cultivation method can reach the commodity export standard.
Example 2 cultivation method of Chamomile
A cultivation site: shaoxing city horizontal base in Zhejiang province
Cultivation time: 10 months in 2018-12 months in 2018
First, the cultivation method of the chrysanthemum indicum
The Japanese chrysanthemum variety "Hongchang" in step 1(2) of example 1 was replaced with the Japanese chrysanthemum variety "Zhan", and the other steps were the same as in step one of example 1.
Second, control group
The Japanese chrysanthemum variety "Hongchang" in the second step of example 1 was replaced with the Japanese chrysanthemum variety "Zhan", and the other steps were the same as the second step of example 1.
Thirdly, the cultivation effect evaluation of the method and the comparison method
Counting the buds within 4cm below the top buds of the chrysanthemum of each of the control group and the experimental group (taking 30 buds of each group) after the reproductive growth period, and comparing the plant type fullness of the two groups of plants.
The comparison results of the number of buds of the chrysanthemum and the whole plant in the control group and the experimental group are shown in FIG. 2. The number of buds counted is as follows: the number of buds within 4cm below the top buds of each chrysanthemum in the control group is 5-6, and the average number is 5.5; the number of buds within 4cm below the top buds of each chrysanthemum in the experimental group reaches 24-29, and the average number is 27; the average number of buds within 4cm below the top buds of each chrysanthemum in the experimental group is obviously more than that in the control group. From the plant type of the whole plant: the plant type fullness of the single chrysanthemum of the experimental group is also obviously superior to that of the control group. The method can effectively improve the bud number and the plant type fullness of the single plant of the chrysanthemum.
In addition, the conditions of the branch length, the branch number, the branch length, the branch bud number and the like of each fresh cut flower of the small chrysanthemum in the experimental group are counted. The results show that: the branch length of each fresh cut flower of the small chrysanthemum cultivated by the method is more than 70cm, the number of branches is 3-4, the length of each branch is 10-15cm, and each branch has at least 5 buds.
The results show that the individual plant of the chrysanthemum obtained by the cultivation method can reach the commodity export standard.
Example 3 cultivation method of Feverfew
A cultivation site: shaoxing city horizontal base in Zhejiang province
Cultivation time: 10 months in 2018-12 months in 2018
First, the cultivation method of the chrysanthemum indicum
The Japanese chrysanthemum variety "Honghuao" in step 1(2) of example 1 was replaced with the Japanese chrysanthemum variety "Xinhongxiao", and the other steps were the same as in step one of example 1.
Second, control group
The Japanese chrysanthemum variety "Honghuai" in the second step of example 1 was replaced with the Japanese chrysanthemum variety "Xinhongxiao", and the other steps were the same as the second step of example 1.
Thirdly, the cultivation effect evaluation of the method and the comparison method
Counting the buds within 4cm below the top buds of the chrysanthemum of each of the control group and the experimental group (taking 30 buds of each group) after the reproductive growth period, and comparing the plant type fullness of the two groups of plants.
The comparison results of the number of buds of chrysanthemum and the whole plant in the control group and the experimental group are shown in FIG. 3. The number of buds counted is as follows: the number of buds within 4cm below the top buds of each chrysanthemum in the control group is 5-6, and the average number is 5.5; the number of buds within 4cm below the top buds of each chrysanthemum in the experimental group reaches 24-29, and the average number is 27; the average number of buds within 4cm below the top buds of each chrysanthemum in the experimental group is obviously more than that in the control group. From the plant type of the whole plant: the plant type fullness of the single chrysanthemum of the experimental group is also obviously superior to that of the control group. The method can effectively improve the bud number and the plant type fullness of the single plant of the chrysanthemum.
In addition, the conditions of the branch length, the branch number, the branch length, the branch bud number and the like of each fresh cut flower of the small chrysanthemum in the experimental group are counted. The results show that: the branch length of each fresh cut flower of the small chrysanthemum cultivated by the method is more than 70cm, the number of branches is 3-4, the length of each branch is 10-15cm, and each branch has at least 5 buds.
The results show that the individual plant of the chrysanthemum obtained by the cultivation method can reach the commodity export standard.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.