CN110168080B - 检测aav的方法 - Google Patents

检测aav的方法 Download PDF

Info

Publication number
CN110168080B
CN110168080B CN201780063531.7A CN201780063531A CN110168080B CN 110168080 B CN110168080 B CN 110168080B CN 201780063531 A CN201780063531 A CN 201780063531A CN 110168080 B CN110168080 B CN 110168080B
Authority
CN
China
Prior art keywords
aav
capsid
gly
pro
ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201780063531.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN110168080A (zh
Inventor
X·金
C·R·奥里奥登
L·刘
K·张
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=59762045&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN110168080(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corp filed Critical Genzyme Corp
Priority to CN202410630268.7A priority Critical patent/CN118688363A/zh
Publication of CN110168080A publication Critical patent/CN110168080A/zh
Application granted granted Critical
Publication of CN110168080B publication Critical patent/CN110168080B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201780063531.7A 2016-08-15 2017-08-14 检测aav的方法 Active CN110168080B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202410630268.7A CN118688363A (zh) 2016-08-15 2017-08-14 检测aav的方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662375314P 2016-08-15 2016-08-15
US62/375,314 2016-08-15
PCT/US2017/046814 WO2018035059A1 (en) 2016-08-15 2017-08-14 Methods for detecting aav

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202410630268.7A Division CN118688363A (zh) 2016-08-15 2017-08-14 检测aav的方法

Publications (2)

Publication Number Publication Date
CN110168080A CN110168080A (zh) 2019-08-23
CN110168080B true CN110168080B (zh) 2024-05-31

Family

ID=59762045

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201780063531.7A Active CN110168080B (zh) 2016-08-15 2017-08-14 检测aav的方法
CN202410630268.7A Pending CN118688363A (zh) 2016-08-15 2017-08-14 检测aav的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202410630268.7A Pending CN118688363A (zh) 2016-08-15 2017-08-14 检测aav的方法

Country Status (23)

Country Link
US (5) US11698377B2 (enExample)
EP (2) EP3497207B1 (enExample)
JP (3) JP7021191B2 (enExample)
KR (5) KR102922101B1 (enExample)
CN (2) CN110168080B (enExample)
AU (2) AU2017312951B2 (enExample)
CA (1) CA3033856A1 (enExample)
CL (2) CL2019000392A1 (enExample)
CR (1) CR20190127A (enExample)
CY (1) CY1124364T1 (enExample)
DK (1) DK3497207T3 (enExample)
ES (1) ES2863674T3 (enExample)
HU (1) HUE053747T2 (enExample)
IL (3) IL264819B2 (enExample)
MA (1) MA55748A (enExample)
MX (2) MX2019001938A (enExample)
NZ (3) NZ751711A (enExample)
PH (1) PH12019500316A1 (enExample)
SG (2) SG10201913002QA (enExample)
TN (1) TN2019000047A1 (enExample)
TW (2) TW202309068A (enExample)
WO (1) WO2018035059A1 (enExample)
ZA (2) ZA201900945B (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201508026D0 (en) 2015-05-11 2015-06-24 Ucl Business Plc Capsid
CA3033856A1 (en) 2016-08-15 2018-02-22 Genzyme Corporation Methods for detecting aav
JOP20190200A1 (ar) 2017-02-28 2019-08-27 Univ Pennsylvania تركيبات نافعة في معالجة ضمور العضل النخاعي
PT3589730T (pt) 2017-02-28 2024-02-28 Univ Pennsylvania Vetor de vírus adenoassociado (aav) de clado f e suas utilizações
IL276859B2 (en) * 2018-02-27 2025-12-01 Univ Pennsylvania Novel adeno-associated virus (aav) vectors, aav vectors having reduced capsid deamidation and uses therefor
EP3768695A4 (en) * 2018-02-27 2022-04-06 The Trustees of the University of Pennsylvania NOVEL ADENO-ASSOCIATED VIRUS (AAV) VECTORS, AAV VECTORS WITH REDUCED CAPSIID DEAMIDATION AND USES THEREOF
EP3762500A1 (en) * 2018-03-06 2021-01-13 Voyager Therapeutics, Inc. Insect cell manufactured partial self-complementary aav genomes
EP4509831A3 (en) * 2018-07-27 2025-03-19 Waters Technologies Corporation Liquid chromatography/mass spectrometry methods for the analysis of polar molecules
WO2020041498A1 (en) * 2018-08-21 2020-02-27 Massachusetts Eye And Ear Infirmary Compositions and methods for modulating transduction efficiency of adeno-associated viruses
IL282411B2 (en) * 2018-10-25 2025-09-01 Regeneron Pharma Methods for analyzing viral capsid protein composition
FI3953483T3 (fi) * 2019-04-11 2023-11-30 Regenxbio Inc Kokoekskluusiokromatografiamenetelmiä rekombinanttien adenoassosioituneiden viruskoostumusten karakterisoimiseksi
US11841352B2 (en) * 2019-07-31 2023-12-12 Thermo Finnigan Llc Determination and correction of retention time and mass/charge shifts in LC-MS experiments
CN114902051B (zh) * 2020-01-03 2026-04-17 萨勒普塔医疗公司 用于分析aav衣壳蛋白的方法
EP4097121A1 (en) 2020-01-29 2022-12-07 Genzyme Corporation Modified adeno-associated viral capsid proteins for ocular gene therapy and methods of use thereof
CN111517359B (zh) * 2020-04-23 2021-08-20 江南大学 一种手性硫化铜超粒子的合成方法
RU2748540C1 (ru) * 2021-02-08 2021-05-26 Автономная некоммерческая образовательная организация высшего образования "Сколковский институт науки и технологий" Способ детектирования вируса SARS-CoV-2 методом масс-спектрометрии
WO2022175728A1 (en) * 2021-02-19 2022-08-25 Waters Technologies Corporation Methods for peptide mapping of adeno-associated virus (aav) proteins
US20240382966A1 (en) * 2021-04-20 2024-11-21 Universal Bio-Sampling Inc. Specimen retention card and specimen inspection method using same
AU2022309726A1 (en) * 2021-07-12 2024-01-25 Regeneron Pharmaceuticals, Inc. Liquid chromatography assay for determining aav capsid ratio
TW202321691A (zh) * 2021-07-12 2023-06-01 美商再生元醫藥公司 用於分析病毒粒子之線上原生質譜方法
CN113552349B (zh) * 2021-07-30 2022-07-19 上海勉亦生物科技有限公司 Aav蛋白外壳的检测方法
JPWO2023132338A1 (enExample) * 2022-01-06 2023-07-13
US20250110119A1 (en) * 2023-03-03 2025-04-03 Sartorius Bioanalytical Instruments, Inc. Methods for quantitating viral capsids
WO2024263520A1 (en) * 2023-06-19 2024-12-26 Regeneron Pharmaceuticals, Inc. Host cell protein analysis for adeno-associated virus (aav)-based gene therapy
WO2025101795A1 (en) * 2023-11-08 2025-05-15 Oxford Biomedica (Us) Llc Deamidation depleted adeno-associated virus product

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1826414A (zh) * 2003-06-30 2006-08-30 加利福尼亚大学董事会 突变型腺伴随病毒的病毒颗粒及其使用方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5540931A (en) 1989-03-03 1996-07-30 Charles W. Hewitt Methods for inducing site-specific immunosuppression and compositions of site specific immunosuppressants
US6204059B1 (en) * 1994-06-30 2001-03-20 University Of Pittsburgh AAV capsid vehicles for molecular transfer
EP0931158A1 (en) 1996-09-06 1999-07-28 The Trustees Of The University Of Pennsylvania An inducible method for production of recombinant adeno-associated viruses utilizing t7 polymerase
US6989264B2 (en) 1997-09-05 2006-01-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6566118B1 (en) 1997-09-05 2003-05-20 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
CA2379166C (en) 1999-08-09 2013-03-26 Targeted Genetics Corporation Enhancement of expression of a single-stranded, heterologous nucleotide sequence from recombinant viral vectors by designing the sequence such that it forms instrastrand base pairs
ATE318923T1 (de) 2000-06-01 2006-03-15 Univ North Carolina Doppelsträngige parvovirus-vektoren
US6723551B2 (en) 2001-11-09 2004-04-20 The United States Of America As Represented By The Department Of Health And Human Services Production of adeno-associated virus in insect cells
MX359371B (es) 2001-11-13 2018-09-25 Univ Pennsylvania Un metodo para detectar y/o identificar secuencias del virus adeno-asociado y aislamiento de secuencias novedosas identificadas de ese modo.
SI1496944T1 (sl) 2002-05-01 2009-02-28 Univ Florida Izboljšani sistemi ekspresije RAAV za genetsko modifikacijo specifičnih kapsidnih proteinov
US20040172200A1 (en) * 2002-11-22 2004-09-02 Kearney Paul Edward Constellation mapping and uses thereof
US7510872B2 (en) 2003-02-26 2009-03-31 Nationwide Children's Hospital Recombinant adeno-associated virus production
US7261544B2 (en) 2003-05-21 2007-08-28 Genzyme Corporation Methods for producing preparations of recombinant AAV virions substantially free of empty capsids
EP1486567A1 (en) 2003-06-11 2004-12-15 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Improved adeno-associated virus (AAV) vector for gene therapy
WO2006119432A2 (en) 2005-04-29 2006-11-09 The Government Of The U.S.A., As Rep. By The Sec., Dept. Of Health & Human Services Isolation, cloning and characterization of new adeno-associated virus (aav) serotypes
EP2311966A3 (en) * 2005-10-20 2012-09-05 Amsterdam Molecular Therapeutics (AMT) B.V. Improved AAV vectors produced in insect cells
US7588772B2 (en) 2006-03-30 2009-09-15 Board Of Trustees Of The Leland Stamford Junior University AAV capsid library and AAV capsid proteins
US20110275529A1 (en) 2008-09-19 2011-11-10 Charite Universitatsmedizin Berlin Identification and characterisation of recombinant viral gene therapy vectors
WO2010093784A2 (en) 2009-02-11 2010-08-19 The University Of North Carolina At Chapel Hill Modified virus vectors and methods of making and using the same
KR20180000341A (ko) 2009-06-16 2018-01-02 젠자임 코포레이션 재조합 ａａｖ 벡터에 대한 개선된 정제 방법
EP2561073B1 (en) 2010-04-23 2016-08-24 University of Massachusetts Cns targeting aav vectors and methods of use thereof
WO2012031245A1 (en) * 2010-09-03 2012-03-08 North Carolina Central University Biodegradable liquogel and ph sensitive nanocarriers
US8663624B2 (en) 2010-10-06 2014-03-04 The Regents Of The University Of California Adeno-associated virus virions with variant capsid and methods of use thereof
TR201908020T4 (tr) 2011-02-17 2019-06-21 Univ Pennsylvania Doku özgüllüğünü değiştirmek ve aav9-aracılı gen transferini geliştirmek için bileşimler ve yöntemler.
ES2857773T5 (es) 2011-08-24 2024-06-04 Univ Leland Stanford Junior Nuevas proteínas de la cápside de AAV para la transferencia de ácidos nucleicos
NZ701693A (en) * 2012-04-18 2017-02-24 The Children’S Hospital Of Philadelphia Composition and methods for highly efficient gene transfer using aav capsid variants
US20140017716A1 (en) * 2012-07-11 2014-01-16 Siscapa Assay Technologies, Inc. Proteolytic digestion kit with dried reagents
GB201401707D0 (en) 2014-01-31 2014-03-19 Sec Dep For Health The Adeno-associated viral vectors
CA2942289C (en) 2014-03-10 2024-05-21 Uniqure Ip B.V. Further improved aav vectors produced in insect cells
CA3033856A1 (en) 2016-08-15 2018-02-22 Genzyme Corporation Methods for detecting aav

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1826414A (zh) * 2003-06-30 2006-08-30 加利福尼亚大学董事会 突变型腺伴随病毒的病毒颗粒及其使用方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Adeno-associated virus capsid serotype identification: Analytical methods development and application;Kim Van Vliet等;《Journal of Virological Methods》;20090326;第159卷;第167页摘要，第169页第2.5节，第175页右栏第2段 *
Characterization of the Capsid Protein Glycosylation of Adeno-Associated Virus Type 2 by High-Resolution Mass Spectrometry;Sarah Murray等;《JOURNAL OF VIROLOGY》;20060630;第80卷(第12期);第6171页摘要，第6173页右栏第4-5段 *
High-Temperature Protein Mass Mapping Using a Thermophilic Protease;Steven J. Bark等;《J. Am. Chem. Soc.》;20011231;第123卷;第1774页左栏第3-4段，右栏第1-2段，图1 *
Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry;Joanna A. Majchrzykiewicz-Koehorst 等;《Journal of Virological Methods》;20141208;第75-83页 *
Resolving Adeno-Associated Viral Particle Diversity With Charge Detection Mass Spectrometry;Elizabeth E. Pierson等;《Anal. Chem. 》;20160616;第88卷;第3718-3725页 *

Also Published As

Publication number Publication date
US20210041451A1 (en) 2021-02-11
KR102538037B1 (ko) 2023-05-30
US11698377B2 (en) 2023-07-11
NZ751711A (en) 2025-11-28
MA55748A (fr) 2022-03-02
AU2017312951A1 (en) 2019-04-04
US12298313B1 (en) 2025-05-13
HUE053747T2 (hu) 2021-07-28
BR112019002934A2 (pt) 2019-05-14
EP3497207B1 (en) 2021-01-06
US12123880B2 (en) 2024-10-22
IL264819B2 (en) 2024-10-01
NZ791267A (en) 2025-11-28
SG11201901221YA (en) 2019-03-28
TW202309068A (zh) 2023-03-01
US20250147042A1 (en) 2025-05-08
KR20240172773A (ko) 2024-12-10
ZA202101002B (en) 2024-07-31
US20250027951A1 (en) 2025-01-23
IL312792A (en) 2024-07-01
KR102922101B1 (ko) 2026-02-03
AU2024200349A1 (en) 2024-02-08
US20240044910A1 (en) 2024-02-08
KR20230078834A (ko) 2023-06-02
IL264819B1 (en) 2024-06-01
CL2019000392A1 (es) 2019-05-10
ES2863674T3 (es) 2021-10-11
WO2018035059A9 (en) 2019-03-14
AU2017312951B2 (en) 2024-02-08
ZA201900945B (en) 2021-06-30
KR102738219B1 (ko) 2024-12-05
JP2024023460A (ja) 2024-02-21
JP2022064980A (ja) 2022-04-26
KR20260036296A (ko) 2026-03-16
WO2018035059A1 (en) 2018-02-22
SG10201913002QA (en) 2020-03-30
EP3851449A1 (en) 2021-07-21
KR20190039253A (ko) 2019-04-10
RU2019107207A3 (enExample) 2021-04-29
CL2019002912A1 (es) 2020-03-06
JP7021191B2 (ja) 2022-02-16
KR102425289B1 (ko) 2022-07-27
TN2019000047A1 (en) 2020-07-15
CR20190127A (es) 2019-06-25
JP2019533803A (ja) 2019-11-21
CY1124364T1 (el) 2022-07-22
TW201825898A (zh) 2018-07-16
NZ791263A (en) 2025-11-28
CN118688363A (zh) 2024-09-24
MX2019001938A (es) 2019-07-04
PH12019500316A1 (en) 2019-08-05
IL264819A (enExample) 2019-04-30
IL323969A (en) 2025-12-01
TWI781953B (zh) 2022-11-01
CN110168080A (zh) 2019-08-23
IL312792B2 (en) 2026-03-01
EP3497207A1 (en) 2019-06-19
DK3497207T3 (da) 2021-03-22
IL312792B1 (en) 2025-11-01
RU2019107207A (ru) 2020-09-15
MX2023009081A (es) 2023-08-08
US20250138024A1 (en) 2025-05-01
KR20220107327A (ko) 2022-08-02
CA3033856A1 (en) 2018-02-22

Similar Documents

Publication Publication Date Title
CN110168080B (zh) 检测aav的方法
RU2771622C2 (ru) Способы выявления aav
HK40056223A (en) Methods for detecting aav
HK40115838A (zh) 检测aav的方法
HK40013370A (en) Methods for detecting aav
HK40013370B (zh) 检测aav的方法
HK40009308B (en) Methods for detecting aav
HK40009308A (en) Methods for detecting aav
BR122024011768A2 (pt) Métodos para determinar a heterogeneidade de uma partícula de aav
BR122024011758A2 (pt) Partícula de raav, composição farmacêutica, kit e artigo de fabricação compreendendo a mesma, proteína de capsídeo de aav e métodos para otimizar a estabilidade, montagem ou transdução de uma partícula de raav
BR122024011753A2 (pt) Partícula de raav, composição farmacêutica, kit e artigo de fabricação compreendendo a mesma, proteína do capsídeo de aav, métodos para para otimizar a estabilidade, montagem, ou transdução de partículas de raav em uma célula
BR122024011745A2 (pt) Métodos para determinar o sorotipo de uma partícula viral e para determinar a heterogeneidade de um sorotipo de uma partícula viral
BR112019002934B1 (pt) Método para determinar o sorotipo de uma partícula de vírus adenoassociado (aav) e método para determinar o sorotipo de uma partícula viral

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40013370

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant
TG01 Patent term adjustment
TG01 Patent term adjustment

Free format text: NEW EXPIRY DATE: 20380614