CN110100582B - Cultivation method of gloryvine container seedlings - Google Patents

Cultivation method of gloryvine container seedlings Download PDF

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Publication number
CN110100582B
CN110100582B CN201811343167.2A CN201811343167A CN110100582B CN 110100582 B CN110100582 B CN 110100582B CN 201811343167 A CN201811343167 A CN 201811343167A CN 110100582 B CN110100582 B CN 110100582B
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cutting
branches
gloryvine
spraying
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CN110100582A (en
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林茂
唐庆
李进华
孙开道
孙利娜
陈尔
廖美兰
杜铃
杨舒婷
黄欣
李冰
秦波
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Guangxi Zhuang Autonomous Region Forestry Research Institute
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Guangxi Zhuang Autonomous Region Forestry Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a cultivation method of a gloryvine container seedling, which is characterized by comprising the following steps of: (1) preparing a matrix; (2) cutting the explant; (3) sterilizing; (4) promoting roots; (5) cutting; (6) and (5) managing illumination spraying. The method solves the problems of low propagation survival rate and long growth period of the gloryvine glaucescens in the prior art, breaks through the conventional propagation method, and can realize container seedling cultivation.

Description

Cultivation method of gloryvine container seedlings
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a cultivation method of a gloriosa vine container seedling.
Background
In recent years, three-dimensional greening is a great trend of future landscaping, and the market demand for strong adaptability and good ornamental vine plants is greatly increased. The vine plant is a kind of plant whose stem is slender, can not be upright, and can only be attached to other objects (such as tree and wall) or crawl on the ground to grow, and the vine plant is a plant material commonly used in garden-making, so that the area available for garden greening is smaller and smaller, and the full utilization of climbing plant to make vertical greening is an important way for expanding greening space, increasing urban green quantity, raising integral greening level and improving ecological environment, and its market demand is very large.
Currently, there are several methods for the propagation of liana: seed propagation, cutting propagation, layering propagation and plant division propagation. Wherein, cutting propagation is taken as the main. The cutting medium commonly used in production comprises yellow mud, vermiculite, perlite, peat soil, river sand and the like, and the commonly used medium materials can basically meet the growth requirement of vine plants in the aspects of physical and chemical properties such as porosity, gas-water ratio, pH value and the like, but have the following defects in the operation process: 1. the water retention time of the matrix is short, the watering frequency is frequent, and the nutrient loss is serious; 2. the substrate is easy to harden, and the water and fertilizer retention capacity is greatly reduced; 3. the survival rate of the cutting seedlings is low, and the industrial production of liana plants is limited.
The blazing vine, the scientific name of Vernonia ellitica DC, is a vine plant of Vernonia of Compositae, has soft lines, fresh and elegant posture, is elegant, strong in adaptability, barren-resistant, rapid in growth, extremely strong in tillering property, extensive in management, capable of being widely popularized and applied to plant configuration for greening balconies, skynes, enclosing walls, guardrails and bare mountains, and is a good material for three-dimensional greening. The method for propagating the gloryvine is mainly cutting propagation, but the survival rate is generally lower than 75%, and the growth cycle is relatively long.
Disclosure of Invention
The invention aims to provide a culture method of a container seedling of gloriosa vines, which aims to solve the problems of low propagation survival rate and long growth period of the gloriosa vines in the prior art, break through the conventional propagation method and realize the culture of the container seedling.
In order to achieve the purpose, the invention adopts the technical scheme that: provide for
A cultivation method of a gloryvine container seedling is characterized by comprising the following steps:
(1) preparing a matrix: uniformly mixing red mud, peat soil, sawdust and perlite according to a proportion, putting into a container, thoroughly leaching with a potassium permanganate aqueous solution, and standing for later use;
(2) cutting an explant: cutting the branches into a section of 10cm long, reserving 2 bud points, cutting the bud points 0.5cm away from the lower end bud point into an inclined plane of 45 degrees, and cutting the inclined plane at a position 1cm away from the upper end bud point;
(3) and (3) disinfection: flattening the lower end of the branch, placing into potassium permanganate aqueous solution, sterilizing the cut for 10min, and air drying for later use;
(4) promoting roots: soaking the disinfected and air-dried branches in a rooting agent solution for 30 min;
(5) cuttage: inserting 1/3 lengths of the branches soaked with the rooting agent into a matrix, compacting and pressing tightly, and thoroughly spraying a carbendazim solution;
(6) and (3) illumination spraying management: spraying for 5 minutes every 1 hour in the daytime, keeping the matrix moist, putting 20 slow release fertilizers for each seedling after the root system completely grows out, and spraying foliar fertilizer and pest control medicine every 7 days; in the illumination spraying management period, the ratio of the light to the water is 6: shading the sun at a point of 00-18: 00, and cooling to 25 ℃ when the illumination exceeds 400001X;
further, the red mud, the peat soil, the sawdust and the perlite are mixed according to a volume ratio of 1:1:1:1, wherein, the sawdust is sealed and fermented for 43-46 days at normal temperature by biogas liquid and zymogen agent before being used.
Further, the mass fraction of the potassium permanganate solution is 0.5%.
Further, the rooting agent is double Gill-GGR with the concentration of 1000 mg/L.
Further, the rooting agent is prepared by uniformly mixing sodium selenite, vitamins, gibberellin and a complex according to the mass part ratio of 10:2:5:5, adding 10 parts of humic acid and 3 parts of a fermenting agent, and fermenting for 10-15 days in a sealed indoor environment.
Further, the rooting agent is prepared by uniformly mixing sodium selenite, vitamins, gibberellin, dichlorophenoxyacetic acid and a complex according to the mass part ratio of 10:2:5:0.3:5, adding 10 parts of humic acid and 2 parts of a fermenting agent, and fermenting indoors in a sealed manner for 10-15 days, wherein the concentration of the dichlorophenoxyacetic acid is 1.5 mg/L.
Further, the foliar fertilizer is a potassium dihydrogen phosphate solution with the mass fraction of 0.1%.
Further, the pest control medicine comprises a carbendazim solution and imidacloprid missible oil; wherein, the carbendazim solution is diluted by 800 times and is sprayed once every 7 days; the imidacloprid missible oil is 5 percent by mass and is sprayed once every 7 days after being diluted by 2000 times.
The invention has the beneficial effects that:
1. compared with the prior art, the invention cultures the gloriosa vine seedling container, and fills the blank of the gloriosa vine seedling container culture technology.
2. The method of the invention breeds the solarium glauca in the container, is beneficial to industrial popularization and application, is beneficial to long-distance transportation, improves the transportation efficiency and reduces the transplanting cost.
3. According to the method, the solamum gloryanum branches are cut into the novel substrate, and the cutting substrate is disinfected in the production process, so that the method is light and clean, and can avoid insect pests and diseases during cutting; the substrate has rich nutrition, strong water absorption and retention capacity and ensures the sufficient water and nutrient supply of the branches; but also can be bred in a container bottle, so that the root system is prevented from extending and penetrating into the soil, the infection rate of surface germs is reduced, and the survival rate is improved.
4. Experiments prove that the method of the invention has short period of the propagation of the gloryvine, simple and convenient operation and high rooting rate; the bred seedlings are neat, light and handy, high in quality and convenient to transport, and can be directly applied after emergence.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more apparent, the present invention is further described in detail below with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
2 months and 1 day in 2018, Guangxi nanning
Culturing the blaze vine seedlings in a container according to the following method, which comprises the following specific steps:
(1) preparing a matrix: mixing red mud: peat soil: fermented sawdust: uniformly mixing perlite according to the volume ratio of 1:1:1:1, putting the perlite into a container of 12cm x 15cm, thoroughly leaching the perlite by using 0.5% potassium permanganate aqueous solution, and standing for 2 days for later use; wherein, the sawdust is sealed and fermented for 43-46 days at normal temperature by biogas liquid and zymophyte agent before use.
(2) Cutting an explant: in 2018, 2, 3 days, cutting healthy and strong lignified or semi-lignified branches into sections with the length of 10cm by using sharp branch scissors, keeping 2 bud points, cutting the bud points 0.5cm away from the lower end into inclined planes with the angle of 45 degrees, and cutting the inclined planes at the positions 1cm away from the upper end bud points;
(3) and (3) disinfection: bundling 50 branches into a bundle by using a rubber band, flattening the lower end, putting into 0.5% potassium permanganate aqueous solution, sterilizing the cut for 10min at the water level height of 5cm, and airing for later use.
(4) Promoting roots: and (3) soaking the aired branches in 1000mg/L double-gill-GGR solution for 30 min.
(5) Cuttage: 1/3 branches are inserted into the sterilized substrate, compacted and pressed tightly, and then drenched with 800 times of carbendazim solution.
(6) Illumination management: the movable sunshade net is set up and can be freely folded and unfolded, the sunshade degree is 75%, the sunshade net is folded from 18:00 o 'clock to 6:00 o' clock of the next day in sunny days and is folded in cloudy days, the sunshade net is opened in the rest of time, and the temperature is reduced to 25 ℃ when the illumination exceeds 400001X.
(7) And (3) water and fertilizer management: the substrate is kept moist, no water is accumulated, no water is poured, the substrate is thoroughly poured, and the substrate is sprayed for 5 minutes every 1 hour in the day to keep the air humidity. After root systems completely grow out in 30 days, 20 slow release fertilizers are put in each seedling, and 0.1% potassium dihydrogen phosphate solution foliar fertilizer is sprayed every 7 days, and the leaves are preferably dripped.
(8) And (3) pest control: spraying 800 times of carbendazim solution every 7 days to prevent and control diseases, and spraying 2000 times of 5% imidacloprid missible oil to prevent and control insect pests.
And counting is carried out by 5 months and 10 days in 2018, 96 surviving seedlings are grown after 60 days, the height of each seedling is over 50cm, the survival rate reaches 96%, no plant diseases and insect pests exist, and the quality of the seedlings is excellent.
Example 2
20 days 4 months in 2018, Guangxi Guilin
(1) Preparing a matrix: mixing red mud: peat soil: fermented sawdust: uniformly mixing perlite according to the volume ratio of 1:1:1:1, putting into a container of 12cm x 15cm, drenching with 0.5% potassium permanganate aqueous solution, standing for 2 days for later use, wherein sawdust is sealed and fermented for 43-46 days at normal temperature by using biogas liquid and fermentation microbial inoculum and then used.
(2) Cutting an explant: cutting healthy and strong lignified or semi-lignified branches into sections with the length of 10cm and the length of 100 sections by using sharp branch scissors in 22 days 4 months 2018, reserving 2 bud points, cutting the bud points 0.5cm away from the lower end into inclined planes with the angle of 45 degrees, and cutting the inclined planes at the positions 1cm away from the upper end;
(3) and (3) disinfection: bundling 50 branches into a bundle by using a rubber band, flattening the lower end, putting into 0.5% potassium permanganate aqueous solution, sterilizing the cut for 10min at the water level height of 5cm, and airing for later use.
(4) Promoting roots: the method comprises the steps of putting the aired branches into 1000mg/L rooting agent solution, soaking for 30min, uniformly mixing sodium selenite, vitamins, gibberellin and a complex according to the mass ratio of 10:2:5:5, adding 10 parts of humic acid and 3 parts of a fermenting agent, fermenting indoors for 10-15 days in a sealed mode, diluting the fermenting liquid with 300 times of clear water, and then soaking the branches.
(5) Cuttage: 1/3 branches are inserted into the sterilized substrate, compacted and pressed tightly, and then drenched with 800 times of carbendazim solution.
(6) Illumination management: the movable sunshade net is set up and can be freely folded and unfolded, the sunshade degree is 75%, the sunshade net is folded from 18:00 o 'clock to 6:00 o' clock of the next day in sunny days and is folded in cloudy days, the sunshade net is opened in the rest of time, and the temperature is reduced to 25 ℃ when the illumination exceeds 400001X.
(7) And (3) water and fertilizer management: the substrate is kept moist, no water is accumulated, no water is poured, the substrate is thoroughly poured, and the substrate is sprayed for 5 minutes every 1 hour in the day to keep the air humidity. After root systems completely grow out in 30 days, 20 slow release fertilizers are put in each seedling, and 0.1% potassium dihydrogen phosphate solution foliar fertilizer is sprayed every 7 days, and the leaves are preferably dripped.
(8) And (3) pest control: spraying 800 times of carbendazim solution every 7 days to prevent and control diseases, and spraying 2000 times of 5% imidacloprid missible oil to prevent and control insect pests.
And 7, 2018, 7, 20 days are counted, and after the growth of 60, the height of the plant is more than 50cm, the survival rate is 100%, the plant is free of plant diseases and insect pests, and the quality of the nursery stock is excellent.
Example 3
7/month/5/2018, Guangxi Baicai
(1) Preparing a matrix: mixing red mud: peat soil: fermented sawdust: uniformly mixing perlite according to the volume ratio of 1:1:1:1, putting into a container of 12cm x 15cm, drenching with 0.5% potassium permanganate aqueous solution, standing for 2 days for later use, wherein sawdust is sealed and fermented for 43-46 days at normal temperature by using biogas liquid and fermentation microbial inoculum and then used.
(2) Cutting an explant: in 2018, 7 months and 7 days, shearing healthy and strong lignified or semi-lignified branches into a section with the length of 10cm by using sharp branch shears, wherein the length of the section is 100 sections, reserving 2 bud points, shearing the bud points 0.5cm away from the lower end into an inclined plane with the angle of 45 degrees, and shearing the inclined plane at the position 1cm away from the upper end;
(3) and (3) disinfection: bundling 50 branches into a bundle by using a rubber band, flattening the lower end, putting into 0.5% potassium permanganate aqueous solution, sterilizing the cut for 10min at the water level height of 5cm, and airing for later use.
(4) Promoting roots: 50 sections of the aired branches are put into 1000mg/L double Gill-GGR solution to be soaked for 30 min. And the other 50 segments are put into another rooting agent solution, and the rooting agent solution is prepared from sodium selenite, vitamins, gibberellin, dichlorophenoxyacetic acid (the concentration is 1.5mg/L) and a complex compound according to the mass part ratio of 10:2:5: mixing uniformly at a ratio of 0.3:5, adding 10 parts of humic acid and 3 parts of leaven, and fermenting in a sealed room for 10-15 days.
(5) Cuttage: 1/3 branches are inserted into the sterilized substrate, compacted and pressed tightly, and then drenched with 800 times of carbendazim solution.
(6) Illumination management: the movable sunshade net is set up and can be freely folded and unfolded, the sunshade degree is 75%, the sunshade net is folded from 18:00 o 'clock to 6:00 o' clock of the next day in sunny days and is folded in cloudy days, the sunshade net is opened in the rest of time, and the temperature is reduced to 25 ℃ when the illumination exceeds 400001X.
(7) And (3) water and fertilizer management: the substrate is kept moist, no water is accumulated, no water is poured, the substrate is thoroughly poured, and the substrate is sprayed for 5 minutes every 1 hour in the day to keep the air humidity. After root systems completely grow out in 30 days, 20 slow release fertilizers are put in each seedling, and 0.1% potassium dihydrogen phosphate solution foliar fertilizer is sprayed every 7 days, and the leaves are preferably dripped.
(8) And (3) pest control: spraying 800 times of carbendazim solution every 7 days to prevent and control diseases, and spraying 2000 times of 5% imidacloprid missible oil to prevent and control insect pests.
The statistics is carried out in 2018, 10, 8 and 8 days, after the growth of 60 days, the plant height is more than 50cm, the survival rate reaches 99 percent, no plant diseases and insect pests exist, and the quality of the nursery stocks is excellent.
In the invention, the foliar fertilizer can also be an amino acid foliar fertilizer or other foliar fertilizers which are commonly sold in the market.
In conclusion, compared with the prior art, the method for cultivating the gloriosa vine seedling container fills the blank of the gloriosa vine seedling container seedling cultivation technology. The method of the invention breeds the solarium glauca in a container, is beneficial to industrial popularization and application, and is beneficial to long-distance transportation. According to the method, the solamum gloryanum branches are cut into the novel substrate, and the cutting substrate is disinfected in the production process, so that the method is light and clean, and can avoid insect pests and diseases during cutting; the substrate has rich nutrition, strong water absorption and retention capacity and ensures the sufficient water and nutrient supply of the branches; but also can be bred in a container bottle, so that the root system is prevented from extending and penetrating into the soil, the infection rate of surface germs is reduced, and the survival rate is improved. Experiments prove that the method of the invention has short period of the propagation of the gloryvine, simple and convenient operation and high rooting rate; the bred seedlings are neat, light and handy, high in quality and convenient to transport, and can be directly applied after emergence.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (4)

1. A cultivation method of a gloryvine container seedling is characterized by comprising the following steps:
(1) preparing a matrix: uniformly mixing red mud, peat soil, sawdust and perlite according to a proportion, putting into a container, thoroughly leaching with a potassium permanganate aqueous solution, and standing for later use; the red mud, the peat soil, the sawdust and the perlite are mixed according to the volume ratio of 1:1:1:1, mixing, wherein sawdust is subjected to sealed fermentation for 43-46 days at normal temperature through biogas liquid and a fermentation microbial inoculum and then used;
(2) cutting an explant: cutting the branches into a section of 10cm long, reserving 2 bud points, cutting the branches into an inclined plane of 45 degrees at a distance of 0.5cm from the bud point at the lower end, and cutting the branches flat at a distance of 1cm from the bud point at the upper end;
(3) and (3) disinfection: flattening the lower end of the branch, placing into potassium permanganate aqueous solution, sterilizing the cut for 10min, and air drying for later use;
(4) promoting roots: soaking the disinfected and air-dried branches in a rooting agent solution for 30 min;
(5) cuttage: inserting 1/3 lengths of the branches soaked with the rooting agent into a matrix, compacting and pressing tightly, and thoroughly spraying a carbendazim solution;
wherein the rooting agent is prepared by uniformly mixing sodium selenite, vitamins, gibberellin and a complex according to the mass part ratio of 10:2:5:5, adding 10 parts of humic acid and 3 parts of a fermenting agent, and fermenting indoors for 10-15 days in a sealed manner;
or; the rooting agent is prepared by uniformly mixing sodium selenite, vitamins, gibberellin, 1.5mg/L dichlorophenoxyacetic acid and a complex according to the mass part ratio of 10:2:5:0.3:5, adding 10 parts of humic acid and 2 parts of a fermenting agent, and fermenting indoors for 10-15 days in a sealed manner;
(6) and (3) illumination spraying management: spraying for 5 minutes every 1 hour in the daytime, keeping the matrix moist, putting 20 slow release fertilizers for each seedling after the root system completely grows out, and spraying foliar fertilizer and pest control medicine every 7 days; in the illumination spraying management period, the ratio of the light to the water is 6: shading the sun at a point of 00-18: 00, and cooling to 25 ℃ when the illumination exceeds 400001X; wherein the pest control medicine comprises carbendazim solution and imidacloprid missible oil; wherein, the carbendazim solution is diluted by 800 times and is sprayed once every 7 days; the imidacloprid missible oil is 5 percent by mass and is sprayed once every 7 days after being diluted by 2000 times.
2. A cultivation method of a container seedling of gloryvine stems as claimed in claim 1, wherein the mass fraction of the potassium permanganate aqueous solution is 0.5%.
3. The method for culturing a container seedling of gloryvine according to claim 1, wherein the rooting agent is bigill-GGR at a concentration of 1000 mg/L.
4. A method for cultivating a container seedling of gloryvine stems as claimed in claim 1, wherein the foliar fertilizer is a potassium dihydrogen phosphate solution with a mass fraction of 0.1%.
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