CN110089296A - The method for establishing tobacco black shank resistance identification sick nursery - Google Patents
The method for establishing tobacco black shank resistance identification sick nursery Download PDFInfo
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- CN110089296A CN110089296A CN201910292886.4A CN201910292886A CN110089296A CN 110089296 A CN110089296 A CN 110089296A CN 201910292886 A CN201910292886 A CN 201910292886A CN 110089296 A CN110089296 A CN 110089296A
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- disease
- cigarette strain
- shank
- balck shank
- plot
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
Abstract
The invention discloses a kind of methods for establishing tobacco black shank resistance identification sick nursery, the flat plot of suitable planting tobacco is selected first, susceptible balck shank kind is chosen to be planted, it is inoculated with balck shank germ, when the mature later period, it after taking Field diseased plant stalk to be crushed, is uniformly sprinkling upon in sick nursery plot, then carries out turning over earthing；Second year, susceptible balck shank kind anti-kind in is chosen to be inoculated with respectively and be not inoculated with balck shank germ, it is inoculated with identical with nonvaccinated Variety Disease-resistance grade, the plot is used as balck shank sick nursery to carry out black shank fastness identification to tobacco bred and uses, grade is different, continue to fall ill and uniformly spread after cigarette strain stalk is crushed to sick nursery plot and carry out turning over earthing, field plot test is identical to grade again.The environmental restrictions factor such as more than long the time required to the present invention overcomes sick nursery foundation, plot selection requirement height and unstable factor provides and quickly establishes the method that the black shank fastness that accuracy is high, repeatability is strong identifies sick nursery.
Description
Technical field
The present invention relates to tobacco disease resistance breeding fields, and in particular to a kind of side for establishing tobacco black shank resistance identification sick nursery
Method.
Background technique
It usually requires to establish stable and accurate method of resistance identification during breeding for disease resistance, to select to resist
Sick breeding material, establishing sick nursery is the important channel that traditional tobacco breeding carries out Disease Resistance Identification, by tobacco breeding material kind
It is implanted in the sick nursery under grown in field environment, is traditional tobacco breeding for disease resistance in conjunction with artificial infection and spontaneous induction morbidity identification
The main method of use.
Currently, the sick nursery foundation for Resistance Identification is the most important thing of accurate breeding for disease resistance.Usual field sick nursery is built
The serious multiple plot of vertical more options disease, it is this meet establish specification sick nursery plot it is not only few, but also since disease is multiple
Plot disease species it is more, germ content type is more in soil and single germ amount is insufficient, on the identification of single disease influence compared with
Greatly, for this purpose, on the plot of the suitable planting tobacco of block one by one in office, the single germ amount of artificial increase in field, and then fast run-up
The black shank fastness that vertical accuracy is high, repeatability is strong identifies sick nursery.
Summary of the invention
Tobacco black shank resistance identification disease is established it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of
The method in garden, this method have the characteristics that accuracy is high, repeatability is strong.
To achieve the above object, a kind of method for establishing tobacco black shank resistance identification sick nursery designed by the present invention, including
Following steps:
1) the flat plot for selecting suitable planting tobacco, chooses susceptible balck shank kind and is planted；
2) when cigarette strain grows to the prosperous growth phase, start to be inoculated with balck shank germ in cigarette strain stem；Maturation is grown to cigarette strain
When the later period (when as the morbidity of cigarette strain balck shank reaches peak period), after taking Field diseased plant stalk to be crushed, it is uniformly sprinkling upon sick nursery
In plot, then carry out turning over earthing；
3) second year, choose susceptible balck shank kind and/or in anti-kind planted；When cigarette strain grows to the prosperous growth phase
When, when cigarette strain grows to the mature later period (when as the morbidity of cigarette strain balck shank reaches peak period), each kind odd number row or double
The cigarette strain stem of number row is inoculated with balck shank germ；
4) cigarette strain of observation inoculation balck shank germ and nonvaccinated cigarette strain incidence, investigate balck shank disease incidence and hair
Sick grade (as unit of strain), calculates disease index, determines resistance class according to disease index；
5) cigarette strain of inoculation balck shank germ and nonvaccinated cigarette strain disease index in more same kind:
When inoculation balck shank germ cigarette strain and nonvaccinated cigarette strain disease index in same interval value, that is, judge them
Balck shank state of an illness rank is consistent, meets known resistance class, continues to fall ill after cigarette strain stalk is crushed and uniformly spread to disease
Garden plot simultaneously carries out turning over earthing, at this point, the plot is used as balck shank sick nursery to carry out black shank fastness identification to tobacco bred
It uses；
When inoculation balck shank germ cigarette strain and nonvaccinated cigarette strain disease index not in same interval value, that is, judge it
Balck shank state of an illness rank it is inconsistent, do not meet known disease-resistant grade, continue that diseased plant stalk is taken to be crushed, be uniformly sprinkling upon sick nursery
It in plot, then carries out turning over earthing, continues to plant identical susceptible kind and/or middle disease-resistant variety repetitive identified, until meeting symbol
Conjunction condition can be used as black shank fastness identification sick nursery.
Further, in the step 1), the tobacco bred of susceptible balck shank is selected from Burley67, Kentucky14 and Hubei Province
White 012.
Still further, in the step 3), in the tobacco bred of anti-balck shank be selected from Tennessee90, build and select 304 and
E'yan 1.
Still further, in the step 4), balck shank disease incidence and sick grade of falling ill calculate disease index, specific steps
It is as follows:
A. investigation 1 time before inoculation, investigation in every 5 days is primary after inoculation, investigates 5 times altogether, investigates whole cigarette strain incidences；
B. severity Scaling method:
0 grade: complete stool is disease-free；1 grade: stem's scab is no more than the one third of stem girth；3 grades: stem's scab is around stem girth
One third is to half；5 grades: stem's scab is more than the half of stem girth；7 grades: stem's scab is entirely around stem girth；9
Grade: diseased plant is substantially withered.
C. Evaluation standard of resistance
R (disease-resistant): 20 or less disease index；MR (in resist): disease index 20.1~40；MS (middle sense): disease index
40.1~60；S (susceptible): disease index 60.1~80；HS (susceptible): disease index 80.1~100；
D. to investigate resulting data, the disease index of each kind is calculated as follows:
Beneficial effects of the present invention:
The environmental restrictions such as more than long the time required to the present invention overcomes sick nursery foundation, plot selection requirement height and unstable factor
Factor provides the method for quickly establishing the black shank fastness identification sick nursery that accuracy is high, repeatability is strong.
Specific embodiment
The present invention is described in further detail combined with specific embodiments below, so as to those skilled in the art understand that.
The method for establishing tobacco black shank resistance identification sick nursery, comprising the following steps:
1) test sick nursery plot is located at Hubei Province somewhere, for 2 mu of area of examination；Test plot topography is flat, and quality is loose, soil
Earth middle fertility, illumination is sufficient, and drainage is good, suitable planting tobacco；
2) disease-resistant rank is divided into according to disease index: being susceptible when a. disease index is 80.1-100；
B. when disease index is 60.1-80, for sense；It c. is middle sense when disease index is 40.1-60；
D. disease index be 20.1-40 when, be in resist；It e. is anti-when disease index is 0.1-20
By it is upper choose plant susceptible No. 0 microspecies of balck shank physiology and No. 1 microspecies of physiology tobacco bred Burley67,
Kentucky14, every two row intervals plantation, each 1000 plants of plantation；
3) start to be inoculated with balck shank germ in cigarette strain stem at the beginning of 6 months when cigarette strain grows to the prosperous growth phase；Wherein, a line is inoculated with
No. 0 biological strain of balck shank, a line are inoculated with No. 1 biological strain, and (as cigarette strain balck shank is sent out when cigarette strain grows to the mature later period
When disease reaches peak period), it after taking Field diseased plant stalk to be crushed, is uniformly sprinkling upon in sick nursery plot, then carry out turning over earthing；
4) second year is chosen according to disease-resistant rank and plants susceptible kind Burley67, Kentucky14, E Bai 012, in resist
Kind (is) E'yan 1, builds and select 304 and 27039 (plantation specification is 120cm × 45cm), when cigarette strain grows to the prosperous growth phase, to
When cigarette strain grows to the mature later period (when as the morbidity of cigarette strain balck shank reaches peak period), each kind odd number row or even numbers row's
Cigarette strain stem is inoculated with balck shank germ；
5) cigarette strain of observation inoculation balck shank germ and nonvaccinated cigarette strain incidence, and investigate after fine day noon black
Shin disease disease incidence and sick grade (as unit of strain) of falling ill, calculate disease index；
Specific step is as follows:
A. investigation 1 time before inoculation, investigation in every 5 days is primary after inoculation, investigates 5 times altogether, investigates whole cigarette strain incidences；
B. severity Scaling method:
0 grade: complete stool is disease-free；1 grade: stem's scab is no more than the one third of stem girth；3 grades: stem's scab is around stem girth
One third is to half；5 grades: stem's scab is more than the half of stem girth；7 grades: stem's scab is entirely around stem girth；9
Grade: diseased plant is substantially withered.
C. Evaluation standard of resistance
R (disease-resistant): 20 or less disease index；MR (in resist): disease index 20.1~40；MS (middle sense): disease index
40.1~60；S (susceptible): disease index 60.1~80；HS (susceptible): disease index 80.1~100；
D. to investigate resulting data, the disease index of each kind is calculated as follows:
6) cigarette strain of inoculation balck shank germ and nonvaccinated cigarette strain disease index in more same kind:
1 balck shank crop field qualification result of table
As shown in table 1, three susceptible kind Burley67, Kentucky14, E Bai are calculated by field observation and investigation
012 is being inoculated with No. 0 microspecies of No. 1 microspecies of physiology and physiology and be not inoculated with the balck shank morbidity disease index and resistance class of processing,
The result shows that: each kind disease refers to that value is unanimous on the whole, within the scope of 80.1-100, disease-resistant grade be it is susceptible, with previous identification
As a result consistent, in three it is anti-kind Burley67, Kentucky14, E Bai's 012 the result shows that, each kind is inoculated with physiology 1
Number No. 0 microspecies of microspecies and physiology and it is not inoculated with the balck shank morbidity disease index of processing and resistance class disease refers to that value is unanimous on the whole,
Disease-resistant grade resists in being, consistent with previous qualification result.
Therefore, it uniformly spreads after for the year being crushed the cigarette strain stalk of three susceptible kinds of morbidity to sick nursery plot and goes forward side by side
Row turns over earthing, which is used as balck shank sick nursery to carry out black shank fastness identification to tobacco bred and uses.
Other unspecified parts are the prior art.Although above-described embodiment is made that the present invention and retouches in detail
State, but it is only a part of the embodiment of the present invention, rather than whole embodiments, people can also according to the present embodiment without
Other embodiments are obtained under the premise of creativeness, these embodiments belong to the scope of the present invention.
Claims (4)
1. a kind of method for establishing tobacco black shank resistance identification sick nursery, it is characterised in that: the following steps are included:
1) the flat plot for selecting suitable planting tobacco, chooses susceptible balck shank kind and is planted；
2) when cigarette strain grows to the prosperous growth phase, start to be inoculated with balck shank germ in cigarette strain stem；The mature later period is grown to cigarette strain
When, it after taking Field diseased plant stalk to be crushed, is uniformly sprinkling upon in sick nursery plot, then carry out turning over earthing；
3) second year, choose susceptible balck shank kind and/or in anti-kind planted；When cigarette strain grows to the prosperous growth phase, to
When cigarette strain grows to the mature later period, the cigarette strain stem of each kind odd number row or even numbers row are inoculated with balck shank germ；
4) cigarette strain of observation inoculation balck shank germ and nonvaccinated cigarette strain incidence investigate balck shank disease incidence and morbidity disease
Grade calculates disease index, determines resistance class according to disease index；
5) cigarette strain of inoculation balck shank germ and nonvaccinated cigarette strain disease index in more same kind:
When inoculation balck shank germ cigarette strain and nonvaccinated cigarette strain disease index in same interval value, that is, judge their black shins
State of an illness rank is consistent, meet it is known susceptible or in anti-resistance class, the cigarette strain stalk that continues fall ill is uniform after being crushed
It spreads to sick nursery plot and carries out turning over earthing, resist at this point, the plot is used as balck shank sick nursery to carry out balck shank to tobacco bred
Property identification use；
When inoculation balck shank germ cigarette strain and nonvaccinated cigarette strain disease index not in same interval value, that is, judge that they are black
Shin disease state of an illness rank is inconsistent, does not meet known disease-resistant grade, continues that diseased plant stalk is taken to be crushed, be uniformly sprinkling upon sick nursery plot
In, then carry out turning over earthing, continue to plant identical susceptible kind and/or middle disease-resistant variety repetitive identified, meets item until meeting
Part can be used as black shank fastness identification sick nursery.
2. establishing the method for tobacco black shank resistance identification sick nursery according to claim 1, it is characterised in that: the step 1)
In, the tobacco bred of susceptible balck shank is selected from Burley67, Kentucky14 and E Bai 012.
3. establishing the method for tobacco black shank resistance identification sick nursery according to claim 1, it is characterised in that: the step 3)
In, in anti-balck shank tobacco bred be selected from Tennessee90, build and select 304 and E'yan 1.
4. establishing the method for tobacco black shank resistance identification sick nursery according to claim 1, it is characterised in that: the step 4)
In, balck shank disease incidence and sick grade of falling ill calculate disease index, the specific steps are as follows:
A. investigation 1 time before inoculation, investigation in every 5 days is primary after inoculation, investigates 5 times altogether, investigates whole cigarette strain incidences；
B. severity Scaling method:
0 grade: complete stool is disease-free；1 grade: stem's scab is no more than the one third of stem girth；3 grades: stem's scab surround three points of stem girth
One of to half；5 grades: stem's scab is more than the half of stem girth；7 grades: stem's scab is entirely around stem girth；9 grades: disease
Strain is substantially withered.
C. resistance class evaluation criterion
R (disease-resistant): 20 or less disease index；MR (in resist): disease index 20.1~40；MS (middle sense): disease index 40.1~
60；S (susceptible): disease index 60.1~80；HS (susceptible): disease index 80.1~100；
D. to investigate resulting data, the disease index of each kind is calculated as follows:
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111727784A (en) * | 2020-07-27 | 2020-10-02 | 贵州省烟草科学研究院 | Method for forecasting tobacco black shank initial period |
| CN113966704A (en) * | 2021-10-27 | 2022-01-25 | 云南省烟草公司大理州公司 | Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety |
| CN114747445A (en) * | 2022-04-29 | 2022-07-15 | 湖北恩施中国南方马铃薯研究中心 | Method for establishing potato bacterial wilt resistance identification garden |
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| CN113966704A (en) * | 2021-10-27 | 2022-01-25 | 云南省烟草公司大理州公司 | Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety |
| CN114747445A (en) * | 2022-04-29 | 2022-07-15 | 湖北恩施中国南方马铃薯研究中心 | Method for establishing potato bacterial wilt resistance identification garden |
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Granted publication date: 20210601 |