CN105850717A - Method for acquiring non-heading Chinese cabbage clubroot resistance germplasm resources - Google Patents
Method for acquiring non-heading Chinese cabbage clubroot resistance germplasm resources Download PDFInfo
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- CN105850717A CN105850717A CN201610248555.7A CN201610248555A CN105850717A CN 105850717 A CN105850717 A CN 105850717A CN 201610248555 A CN201610248555 A CN 201610248555A CN 105850717 A CN105850717 A CN 105850717A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a method for acquiring non-heading Chinese cabbage clubroot resistance germplasm resources. The method comprises the following steps of selecting introduced Chinese cabbages with clubroot resistance and susceptible non-heading Chinese cabbages with excellent agronomic characters; carrying out interspecific hybridization in a bud stage, and acquiring seeds of a hybrid generation F1; successively backcrossing 4 generations by the selected F1, and initially acquiring non-heading Chinese cabbage resistance new germplasm Q15-1 with stable phenotype and clubroot resistance. According to the method for acquiring the non-heading Chinese cabbage clubroot resistance germplasm resources, the method of interspecific hybridization is adopted, each backcrossing generation is artificially inoculated and identified, a resistant individual plant with clubroot resistance is selected, and a new germplasm is finally acquired. The hybrid vigor between the Chinese cabbages in cruciferae brassica species and the varietal non-heading Chinese cabbages thereof is utilized, and resistance genes of the Chinese cabbages with clubroot resistance are transferred into the non-heading Chinese cabbages through hybridization in the bud stage and a backcrossing transfer technique, so that the method is simple and easy to implement, and biohazards caused by other manners such as transgenosis and gene engineering technologies are eliminated.
Description
Technical field
The invention belongs to variety of crops germplasm innovation and develop field, particularly relate to a kind of anti-of Chinese cabbage
The method that swollen sick germ plasm resource obtains.
Background technology
Crop in cruciferae clubroot is to be soaked by plasmodiophora brassica bacteria (plasmodiophora brassicae Woron)
The crop in cruciferae root disease that dye causes, this pathogenic bacteria is a kind of obligate parasite, contaminates cruciate flower specially
Section crop.This pathogen invaded plants root hair, cortex, cambium layer, produce the disease containing auxin
Bacterium secretions, stimulate parenchyma cell abnormal division increase, expand at hypertrophy position formed warty daetylorhiz.
On the one hand the appearance of daetylorhiz reduces Root Absorption and supply moisture content and the ability of nutrient, causes aerial parts to lose
Water, withered and yellow and dead；On the other hand root system of plant is made to lose the protective effect of outer layer, easily by other miscellaneous bacteria
Invade and cause rotten.
Clubroot can endanger the cultivation of multiple Cruciferae and the open countries such as Chinese cabbage, green vegetable, Brassica oleracea L. var. botrytis L., Caulis et Folium Brassicae capitatae, Brassica campestris L
Raw plant, once occurs, and will result in serious harm.In China, this disease occurs mainly in East China and south China
The major production areas of some crop in cruciferae.Crop in cruciferae clubroot infectiousness is extremely strong, can with seed,
The number of ways such as fertilizer, rainwater, irrigation water, insect pest, the plague of rats, farming operation are propagated, and pathogen is at soil
Or survive the winter on seed, can survive in soil more than 6 years, pathogenic bacteria all can grow at 9～23 DEG C, soil water-containing
Amount 70%～90% susceptible disease, this disease spread speed soon, onset area between short several years in tens times,
Even hundreds of times increase, and endanger extremely serious.
Chinese cabbage (Brassic campestris ssp.chinensis Makino) has another name called Plantula Brassicae chinensis, green vegetable,
Belong to Cruciferae Brassica genus, because its quality is tender, unique flavor, the feature such as nutritious, in people's lives
In occupy an important position, especially in Shanghai, the middle and lower reach of Yangtze River and areas to the south, Chinese cabbage is people
Popular vegetable that every day must eat, indispensable.But in recent years, suburb of Shanghai, as Qingpu, Songjiang,
The ground Chinese cabbage clubroot morbidity such as Kingsoft and Pudong increases the weight of year by year, and serious region yield drastically declines,
Even return product, affect the regular supply in Chinese cabbage market, cause extreme loss.Generally, balling is not white
A large amount of hypertrophy that after dish morbidity, root tissue is abnormal, expand at hypertrophy position and form the daetylorhiz of warty, going out of daetylorhiz
Now compromising growth and the absorbability of root system, the growth and development of plants infected is slow, make plant wilt,
Yellow and fallen leaves, the most whole strain is dead.Will result in yield after green vegetable are susceptible to decline, quality reduces, soft time serious
Rotten antibacterial and other pathogen are taken advantage of a weak point, and infect the position that is injured, and No kernels or seeds are gathered, as in a year of scarcity finally to make plant withered death,
Loss greatly is caused to vegetable grower.
Chinese cabbage and Chinese cabbage are different mutation, the research to green vegetable clubroot in same Chinese cabbage subspecies
Chinese cabbage, the most sick to the inoculation of Chinese cabbage knee occurrence regularity, biological strain, inoculation mirror can be used for reference
Determine method, selection-breeding transformation and molecular biosciences repeatedly carry out research report.Tan, Huang, condition etc. have studied DABAI
Resistance Identification and Resistance Physiology analysis to clubroot between colza.By the anti-clubroot to Chinese cabbage such as king, grandson
Heredity carry out preliminary study, confirm that Chinese cabbage anti-clubroot heredity has pair of dominant genes control.In breeding
Aspect, because the most anti-source in Chinese cabbage, Ji Chuan etc. (1977) is by miscellaneous with disease-resistant Radix Brassicae rapae for Chinese cabbage
Hand over, with Chinese cabbage, the cenospecies of Chinese cabbage and Radix Brassicae rapae is carried out 4～5 times and backcross, it is possible to obtain character is excellent
Good, and there is the Chinese cabbage of resistance.The method, Japan, Korea S etc. is utilized the most in succession to obtain anti-clubroot
Chinese cabbage cultivar, domestic, Sun Baoya etc. isolates the anti-clubroot of Chinese cabbage certainly from the external Chinese cabbage introduced
Friendship is 48.At present in Qingdao, the area such as Yunnan and Shenyang obtained the Chinese cabbage material of anti-clubroot,
Open to wait and obtain Chinese cabbage anti-clubroot new varieties CR589 by selection-breeding.The kind of the anti-clubroot of Chinese cabbage
Matter resource innovation research rarely has report, does not more have the anti-clubroot green vegetable cultivar of molding.Therefore, by
Hybridization between distant hybridization or mutation, it is thus achieved that the main breeding mode of Resistant gerplasm resource, will resist for green vegetable
Clubroot breeding work provides new germ plasm resource.
Summary of the invention
It is an object of the invention to provide the method that the germ plasm resource of the anti-clubroot of a kind of Chinese cabbage obtains,
Aim to solve the problem that the method lacking the initiative about Chinese cabbage anti-clubroot germ plasm resource in prior art
Problem with the anti-clubroot green vegetable cultivar not having molding.
The present invention is achieved in that the method that the germ plasm resource of the anti-clubroot of a kind of Chinese cabbage obtains,
The method comprises the steps:
Step one, introduce the Chinese cabbage of anti-clubroot, and use artificial inoculation on seedling authentication method to screen agronomy
The susceptible Chinese cabbage homozygous inbred lines that character is excellent；
Step 2, in florescence, with susceptible Chinese cabbage as female parent, the Chinese cabbage of anti-clubroot is male parent,
Carry out shelling the intervarietal hybridization of flower bud, it is thus achieved that the seed of first-filial generation F1；
Step 3, is carried out the Resistance Identification of F1 generation clubroot, and screens anti-clubroot by Inoculation Method
Plant；
Step 4, with screening F1 generation anti-clubroot plant as female parent, the susceptible not balling that economical character is excellent
Chinese cabbage homozygous inbred lines is male parent, backcrosses；By the anti-clubroot plant of acquisition that backcrosses, by manually connecing
The method of kind carries out the artificial infection idenfication of clubroot；
Step 5, the plant of the anti-clubroot filtered out from backcross progeny is re-used as maternal and susceptible not balling
Chinese cabbage backcrosses, continuous backcross 4 generation, and wherein first backcross generation and second backcross generation are by artificial vernalization
After reason, carrying out adding generation obtains, and per generation all carry out the Resistance Identification of clubroot, screen anti-clubroot individual plant,
Obtain the Chinese cabbage resistance new germ plasm Q15-1 of the anti-clubroot of Phenotype preliminarily stabilised.
Further, the anti-numbered B13-1 of clubroot Chinese cabbage introduced in described step one, economical character is excellent
The good susceptible numbered Q13-1 of Chinese cabbage homozygous inbred lines.
Further, in described step 4 with screening F1 generation anti-clubroot plant as female parent, economical character is excellent
Susceptible Chinese cabbage homozygous inbred lines be male parent, backcross；By the anti-clubroot plant of acquisition that backcrosses
Q15-1。
Further, in step 3 and step 4, Resistance Identification carries out artificial infection idenfication in warmhouse booth, system
Meter sickness rate and disease index.
The present invention method by intervarietal hybridization, enters Chinese cabbage and the susceptible Chinese cabbage of anti-clubroot
Row intervarietal hybridization, the Chinese cabbage utilizing economical character excellent cenospecies is carried out continuous 4 generations backcross turn
Educate, and each backcross progeny is carried out artificial infection idenfication, screen the resistance individual plant of anti-clubroot, finally reach
To the purpose obtaining the stable anti-clubroot Chinese cabbage new germ plasm of Phenotype.What the present invention utilized is cross
The Chinese cabbage of flower section Brassica genus and mutation Chinese cabbage heterosis, hybrid vigor between the two thereof, by anti-clubroot DABAI
The resistant gene of dish passes through hybridization and the backcross transformation technology of flower bud phase, in transformation to Chinese cabbage, the most easily
OK, eliminate other modes such as transgenic, technique for gene engineering etc. and cause biological risk.The present invention is led to
Cross intervarietal hybridization backcross transformation technology, the most only anti-clubroot of Chinese cabbage and formulate new disease-resistant material,
Will be the most significant to the practice of Chinese cabbage breeding for disease resistance and theoretical research thereof；The present invention by system,
Collect, excavate the germ plasm resource of the anti-clubroot of Chinese cabbage group widely, and carry out the arranging in order of resource,
Evaluate and effectively utilize；By the intervarietal hybridization technology that same genus is the most of the same race, the resistance of anti-clubroot is utilized to tie
Ball Chinese cabbage and susceptible Chinese cabbage, carry out the sexual hybridization of flower bud phase, utilizes excellent not the tying of economical character
Ball Chinese cabbage carries out continuous print transformation, wherein have employed low temperature vernalization treatment and carries out artificial increasing generation technique, shortens
The time limit of new germ plasm initiative；In addition combine clubroot Disease Resistance Identification technology filial generation and introgressive line are entered
Row Resistance Identification, for screening and the target of orientation system selection-breeding anti-clubroot Chinese cabbage new varieties, initiative
Go out new germ plasm anti-for Chinese Cabbage clubroot gene is raw by the susceptible Chinese cabbage of sexual hybridization technological sourcing
In object；And utilize hybridization, backcross transformation method and the identification technology of anti-clubroot, recombinate and improve sense
The anti-clubroot gene of sick Chinese cabbage, and drastically increase its resistance to clubroot, it is thus achieved that one
Criticize Chinese cabbage new germ plasm that have value, that anti-clubroot, Horticultural Characters are excellent；For fully excavating
The anti-clubroot gene of Chinese cabbage, further the research anti-clubroot of Chinese cabbage Mechanism of Physiological and Biochemical,
Genetic development and molecular biology thereof are laid a good foundation.
Accompanying drawing explanation
Fig. 1 is the method stream of the germ plasm resource acquisition of the anti-clubroot of Chinese cabbage that the embodiment of the present invention provides
Cheng Tu.
Fig. 2 is the initiative process schematic of the anti-clubroot Chinese cabbage kind matter that the embodiment of the present invention provides.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment,
The present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to
Explain the present invention, be not intended to limit the present invention.
The collection of germ plasm resource, utilize and evaluate premise and the basis being by breeding research, therefore to cross
Extensively collecting and evaluating of flower section clubroot resistant material, is to study Chinese cabbage clubroot characteristic further
And the basis of resistance breeding, the present invention is by excellent with economical character for the Chinese cabbage obtaining introduction anti-clubroot gene
Good susceptible Chinese cabbage hybridization, by not tying of excellent for anti-clubroot channel genes character but the most anti-clubroot
In ball Chinese cabbage body, the modern breeding methods such as employing hybridizes and continuous backcross breeding technique, Disease Resistance Identification technology,
Selection-breeding obtains the most anti-clubroot, Horticultural Characters but also excellent Chinese cabbage new germ plasm.
Below in conjunction with the accompanying drawings the application principle of the present invention is explained in detail.
As shown in Figure 1: the method that the germ plasm resource of the anti-clubroot of a kind of Chinese cabbage obtains, this not balling
The method that the germ plasm resource of the anti-clubroot of Chinese cabbage obtains comprises the steps:
S101: introduce the Chinese cabbage of anti-clubroot, and use artificial inoculation on seedling authentication method to screen economical character
Excellent susceptible Chinese cabbage homozygous inbred lines；
S102: in florescence, with susceptible Chinese cabbage as female parent, the Chinese cabbage of anti-clubroot is male parent, enters
The intervarietal hybridization of row stripping flower bud, it is thus achieved that the seed of first-filial generation F1；
S103: carried out the Resistance Identification of F1 generation clubroot by Inoculation Method, and screen anti-clubroot and plant
Strain；
S104: with the F1 generation anti-clubroot plant of screening as female parent, the excellent susceptible not balling of economical character is white
Dish homozygous inbred lines is male parent, backcrosses；By the anti-clubroot plant of acquisition that backcrosses, pass through artificial vaccination
Method carries out the artificial infection idenfication of clubroot；
S105: it is white that the plant of the anti-clubroot filtered out from backcross progeny is re-used as maternal and susceptible not balling
Dish backcrosses, continuous backcross 4 generation, and wherein first backcross generation and second backcross generation are processed by artificial vernalization
After, carrying out adding generation obtains, and per generation all carry out the Resistance Identification of clubroot, screen anti-clubroot individual plant, just
Step obtains the Chinese cabbage resistance new germ plasm Q15-1 of the stable anti-clubroot of Phenotype.
In described S101 introduce the anti-numbered B13-1 of clubroot Chinese cabbage, economical character excellent susceptible not
The numbered Q13-1 of Chinese cabbage homozygous inbred lines.
In described S104 with screening F1 generation anti-clubroot plant as female parent, economical character excellent susceptible not
Chinese cabbage homozygous inbred lines is male parent, backcrosses；By the anti-clubroot plant Q15-1 of acquisition that backcrosses.
In S103 and S104, Resistance Identification carries out artificial infection idenfication in warmhouse booth, statistics sickness rate and
Disease index.
Below in conjunction with embodiment, the application principle of the present invention is further described.
1 material and method
1.1 material
Chinese cabbage B13-1 be the west quoted from Sheng Nisi seeds company of Korea S by ferrum root, there is anti-clubroot characteristic
Chinese cabbage.
Chinese cabbage Q13-1 by Academy of Agricultural Sciences, Shanghai City gardening the material of selection-breeding for many years, be selfing
The height that 6-10 obtains for selection-breeding is for selfing line, and Comprehensive Traits is excellent, has TuMV, downy mildew and melasma
Stronger resistance, then shows as susceptible to clubroot.
1.2 Disease Resistance Identification
Anti-clubroot Chinese cabbage, susceptible Chinese cabbage germplasm materials and cenospecies, the kind that backcrosses are in different generations
Disease Resistance Identification is carried out during transformation.Clubroot Inoculation Method uses pathogen plasmodiophora bacterium local method, i.e. with newly
The weight ratio 2:5 mixing of fresh knee block and substrate is mixed thoroughly, inserts the foam hole tray in 77 holes, with can under hole tray
The vinyl disc of the concave-convex type being filled with water.Qualification test is carried out in insect protected greenhouse, the strain of every part of material 50, is seeded in bacterium
In soil, repeat, compare with the bright light of anti-clubroot Chinese cabbage material (CK), carry out statistical analysis for 3 times.In
After planting 40d investigates disease index.Field test, is carried out on Qingpu Shanghai great sun farm.Utilize field natural
Source of disease, carries out appraisal survey, random alignment, repeats for 3 times, random searching 100 strain of every community germplasm materials,
Carry out statistical analysis.
1.3 data statistics
Clubroot severity Scaling there is no unified standard, and severity is divided into 4 grades:
Do not fall ill for 0 grade；
1 grade of fibrous root enlargement, or the slight enlargement of main root but inconspicuous；
3 grades of obvious enlargements of main root, tumor size reaches 2～3 times of basal part of stem cross-sectional area；
5 grades of obvious enlargements of main root, tumor size reaches more than 4 times of basal part of stem cross-sectional area, or main, must present many
Individual obvious tumor.
Survey result calculates strain sickness rate and disease index by formula (1)～(2).
Diseased plant rate=(morbidity strain number/total strain number) × 100% ... ... (1)
Disease index=∑ (morbidity level representative value × diseased plant numbers at different levels) × 100/
(investigating total strain number × superlative degree morbidity representative value) ... ... ... (2)
2 results and analysis
The initiative process of 2.1 kinds of matter
The initiative of kind of matter is carried out with the method for intervarietal hybridization, backcross transformation, the anti-clubroot i.e. selecting to introduce big
Chinese cabbage B13-1 and the excellent susceptible Chinese cabbage Q13-1 of economical character；In bolting florescence, with susceptible not
Chinese cabbage is maternal, and the Chinese cabbage of anti-clubroot is male parent, carries out the intervarietal hybridization of flower bud phase, it is thus achieved that hybridization
The seed of generation F1；The F1 generation Resistance Identification by clubroot, screens anti-clubroot plant；F1 with screening
It is maternal for anti-clubroot plant, with the excellent Q13-1 of economical character as male parent, backcrosses；By backcrossing
Obtain BC13-1, carry out the inoculated identification of clubroot, the plant of the anti-clubroot filtered out be re-used as maternal with
Chinese cabbage backcrosses, continuous backcross 4 generation, and wherein 2 generations obtained by manually adding generation, and per generation is all carried out
The Resistance Identification of clubroot, the preliminary Chinese cabbage resistance new germ plasm obtaining the stable anti-clubroot of Phenotype
Q15-1 (see Fig. 2)
2.2 Chinese cabbages and the clubroot inoculated identification result of Chinese cabbage cenospecies
The F1 obtained with resistance Chinese cabbage and susceptible Chinese cabbage intervarietal hybridization and the indoor of parents thereof and field
Qualification result is as follows, and comparison and Chinese cabbage B13-1 are accredited as disease-resistant in indoor and field anti-clubroot, right
The clubroot biological strain in area, Qingpu shows as high anti-.And Chinese cabbage Q13-1, indoor and field
Qualification result sickness rate and disease index reach 53.06% and 65.31% and 25.31 and 36.32, show as susceptible.
And the Hybrid F1 obtained by intervarietal hybridization is at the indoor and sickness rate of field test and disease index the most relatively
Low, cash as disease-resistant.
Table 1 Chinese cabbage and Chinese cabbage and the clubroot Resistance Identification result of cenospecies thereof
The clubroot inoculated identification result that 2.2 Chinese cabbages and Chinese cabbage backcross kind
Between 2013 to 2015 years, by manually adding generation, it is thus achieved that backcross 4 generation seeds, resist according to clubroot
Property qualification result, as shown in Table 2, the backcross progeny of resistance Chinese cabbage and Chinese cabbage is in indoor and field
In qualification, comparison and Chinese cabbage B13-1 are accredited as disease-resistant in indoor and field anti-clubroot, to ground, Qingpu
The clubroot biological strain in district shows as high anti-.Chinese cabbage Q13-1, at the indoor and qualification result in field
Sickness rate and disease index reach 63.43% and 55.35% and 39.61 and 28.93, and BC14-1 is 1 generation that backcrossed,
BC14-2 and BC15-1 is backcross 2 generations and 3 generations that backcrossed that artificial vernalization adds generation, its indoor and field test
Result all shows as disease-resistant. and the sickness rate of each backcross progeny is below 10%, and disease index is less than 10, table
It is now disease-resistant.
Table 2 Chinese cabbage and Chinese cabbage and the clubroot Resistance Identification result of backcross progeny thereof
The present invention passes through system, collects, excavates the germ plasm resource of the anti-clubroot of Chinese cabbage group widely, and
Carry out the arrangement in order of resource, evaluate and effectively utilize.By the intervarietal hybridization technology that same genus is the most of the same race,
Utilize the resistance Chinese Cabbage of anti-clubroot and susceptible Chinese cabbage, carry out the sexual hybridization of flower bud phase, profit
Carry out continuous print transformation with the Chinese cabbage that economical character is excellent, wherein have employed low temperature vernalization treatment and carry out
Artificial increasing generation technique, shortens the time limit of new germ plasm initiative.In addition clubroot Disease Resistance Identification technology pair is combined
Filial generation and introgressive line carry out Resistance Identification, white for screening and orientation system selection-breeding anti-clubroot not balling
The target of dish new varieties, formulates out new germ plasm by anti-for Chinese Cabbage clubroot gene by sexual hybridization technology
Import in susceptible Chinese cabbage organism.And utilize hybridization, backcross transformation method and the qualification of anti-clubroot
Technology, recombinates and improves the anti-clubroot gene of susceptible Chinese cabbage, and drastically increasing it to root
Swollen sick resistance, it is thus achieved that a collection of not balling that have value, that anti-clubroot, Horticultural Characters are excellent is white
Dish new germ plasm.For fully excavating the anti-clubroot gene of Chinese cabbage, research Chinese cabbage resists further
The Mechanism of Physiological and Biochemical of clubroot, genetic development and molecular biology thereof are laid a good foundation.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Any amendment, equivalent and the improvement etc. made within bright spirit and principle, should be included in the present invention
Protection domain within.
Claims (4)
1. the method that the germ plasm resource of the anti-clubroot of Chinese cabbage obtains, it is characterised in that this is not tied
The method that the germ plasm resource of the anti-clubroot of ball Chinese cabbage obtains comprises the steps:
Step one, introduce the Chinese cabbage of anti-clubroot, and use artificial inoculation on seedling authentication method to screen agronomy
The susceptible Chinese cabbage homozygous inbred lines that character is excellent；
Step 2, in florescence, with susceptible Chinese cabbage as female parent, the Chinese cabbage of anti-clubroot is male parent,
Carry out shelling the intervarietal hybridization of flower bud, it is thus achieved that the seed of first-filial generation F1；
Step 3, is carried out the Resistance Identification of F1 generation clubroot, and screens anti-clubroot by Inoculation Method
Plant；
Step 4, with screening F1 generation anti-clubroot plant as female parent, the susceptible not balling that economical character is excellent
Chinese cabbage homozygous inbred lines is male parent, backcrosses；By the anti-clubroot plant of acquisition that backcrosses, by manually connecing
The method of kind carries out the artificial infection idenfication of clubroot；
Step 5, the plant of the anti-clubroot filtered out from backcross progeny is re-used as maternal and susceptible not balling
Chinese cabbage backcrosses, continuous backcross 4 generation, and wherein first backcross generation and second backcross generation are by artificial vernalization
After reason, carrying out adding generation obtains, and per generation all carry out the Resistance Identification of clubroot, screen anti-clubroot individual plant,
The preliminary Chinese cabbage resistance new germ plasm Q15-1 obtaining the stable anti-clubroot of Phenotype.
2. the method that the germ plasm resource of the anti-clubroot of Chinese cabbage as claimed in claim 1 obtains, it is special
Levy and be, the anti-numbered B13-1 of clubroot Chinese cabbage introduced in described step one, excellent easy of economical character
The sense numbered Q13-1 of Chinese cabbage homozygous inbred lines.
3. the method that the germ plasm resource of the anti-clubroot of Chinese cabbage as claimed in claim 1 obtains, it is special
Levy and be, with the F1 generation anti-clubroot plant of screening as female parent in described step 4, excellent easy of economical character
Sense Chinese cabbage homozygous inbred lines is male parent, backcrosses；By the anti-clubroot plant Q15-1 of acquisition that backcrosses.
4. the method that the germ plasm resource of the anti-clubroot of Chinese cabbage as claimed in claim 1 obtains, it is special
Levying and be, in described step 3, step 4 and step 5, Resistance Identification carries out artificial vaccination in warmhouse booth
Identify, statistics sickness rate and disease index.
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Cited By (6)
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| CN106489723A (en) * | 2016-12-02 | 2017-03-15 | 辽宁省农业科学院 | A kind of inbred line breeding method of anti-celery cabbage clubroot |
| CN106508669A (en) * | 2016-11-16 | 2017-03-22 | 青岛市农业科学研究院 | Breeding method of Chinese cabbages resistant to clubroot |
| CN108739147A (en) * | 2018-06-13 | 2018-11-06 | 山东省潍坊市农业科学院 | A method of the anti-clubroot Chinese cabbage germplasm of initiative |
| CN109362505A (en) * | 2018-12-18 | 2019-02-22 | 武汉市农业科学院 | A kind of Chinese cabbage quickly adds the method in generation |
| CN111386788A (en) * | 2020-04-30 | 2020-07-10 | 南京农业大学 | Initiator with disease resistance and insect resistance for small-sized seeds of non-heading Chinese cabbages and application of initiator |
| CN118335187A (en) * | 2024-04-23 | 2024-07-12 | 重庆市农业科学院 | Construction method of lemon disease-resistant gene map based on data mining |
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| CN106508669A (en) * | 2016-11-16 | 2017-03-22 | 青岛市农业科学研究院 | Breeding method of Chinese cabbages resistant to clubroot |
| CN106489723A (en) * | 2016-12-02 | 2017-03-15 | 辽宁省农业科学院 | A kind of inbred line breeding method of anti-celery cabbage clubroot |
| CN106489723B (en) * | 2016-12-02 | 2019-10-22 | 辽宁省农业科学院 | A kind of inbred line breeding method of anti-celery cabbage clubroot |
| CN108739147A (en) * | 2018-06-13 | 2018-11-06 | 山东省潍坊市农业科学院 | A method of the anti-clubroot Chinese cabbage germplasm of initiative |
| CN108739147B (en) * | 2018-06-13 | 2020-09-01 | 山东省潍坊市农业科学院 | Method for creating clubroot-resistant Chinese cabbage germplasm |
| CN109362505A (en) * | 2018-12-18 | 2019-02-22 | 武汉市农业科学院 | A kind of Chinese cabbage quickly adds the method in generation |
| CN111386788A (en) * | 2020-04-30 | 2020-07-10 | 南京农业大学 | Initiator with disease resistance and insect resistance for small-sized seeds of non-heading Chinese cabbages and application of initiator |
| CN111386788B (en) * | 2020-04-30 | 2021-09-14 | 南京农业大学 | Initiator with disease resistance and insect resistance for small-sized seeds of non-heading Chinese cabbages and application of initiator |
| CN118335187A (en) * | 2024-04-23 | 2024-07-12 | 重庆市农业科学院 | Construction method of lemon disease-resistant gene map based on data mining |
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