CN109362505A - A kind of Chinese cabbage quickly adds the method in generation - Google Patents
A kind of Chinese cabbage quickly adds the method in generation Download PDFInfo
- Publication number
- CN109362505A CN109362505A CN201811553171.1A CN201811553171A CN109362505A CN 109362505 A CN109362505 A CN 109362505A CN 201811553171 A CN201811553171 A CN 201811553171A CN 109362505 A CN109362505 A CN 109362505A
- Authority
- CN
- China
- Prior art keywords
- seed
- generation
- chinese cabbage
- plant
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of methods that Chinese cabbage quickly adds generation, the following steps are included: seed sprout after 2-4 °C vernalization treatment 15-25 days, then it is transplanted to greenhouse whole day illumination cultivation under the LED lamp tube of full spectrum, plant controls plant type by way of removing peripheral old leaf and lotus throne leaf after buddingging, main a kind of sedge the flowers are in blossom put after pollinate to the mature bud of inflorescence outermost, the kind skin of immature seed after pollination is removed with breaking dormancy, next generation is after planting entered.This method can greatly accelerate the breeding process of Chinese cabbage new varieties, realize the propagation generation in 4 generation of Chinese cabbage at least a year.
Description
Technical field
The invention belongs to crop breeding fields, and in particular to a kind of to realize that Chinese cabbage quickly adds the new of generation in greenhouse
Method.
Background technique
Chinese cabbage is biennial brassicaceous vegetable, can only breed a generation within 1 year under field conditions (factors).In breeding
A self-mating system is cultivated in the process and generally requires the 6-8 year, and is cultivated a cenospecies and then needed the longer time.In order to mention
The breeding efficiency of high Chinese cabbage, there is an urgent need to shorten its growth cycle, the method for exploring fast generation.Forefathers once attempted strange land
Shuttle breeding, winter greenhouse are collected seed and are bred Chinese cabbage material using means such as tissue cultures, but these methods are equal
It can only achieve the reproductive efficiency in 1 year 1.5-2 generation.Trace it to its cause be Chinese cabbage growth period it is longer, vegetative growth phase need
Experience the influence of vernalization, the later period also needs to incude higher temperature and long-day and could normally bloom, or even mature kind
Son also has stronger dormancy, so the reproductive efficiency of the overlaying influences of these factors Chinese cabbage improves.
Summary of the invention
In order to overcome the shortcomings and deficiencies of the prior art, the present invention provides a kind of quick using greenhouse progress Chinese cabbage
Add the method in generation, this method can greatly accelerate the breeding process of Chinese cabbage new varieties.The method utilizes the kind sprouted
Son carries out vernalization treatment in 2-4 DEG C of environment, is then transplanted under the LED lamp tube of the full spectrum in greenhouse and carries out 24 hours continuous light
According to control plant type and Pollination Modes in incubation, 25 days kind skins by immature seed are removed with breaking dormancy after pollination, are broadcast
Next generation is entered after kind.
To achieve the goals above, the present invention is achieved through the following technical solutions:
(1) seed sprouting and vernalization treatment
It selects full seed to be put into culture dish, water is added to sprout, the ice after be placed into 2-4 DEG C after Seed sprouting
It is handled 15-25 days in case, carries out incomplete vernalization treatment, during which Germinating Seeds is kept to be in the environment moistened.
(2) plant is cultivated
The seed of vernalization treatment is transplanted to greenhouse, is seeded into Nutrition Soil, is placed on tissue culture frame, full light is at
Under the irradiation of the LED lamp tube of spectrum, the spectral band of fluorescent tube is between 340-800nm, whole day illumination cultivation.During culture, every
One time of nutrition liquid was supplemented every two day time.
(3) buds dwarfing
After plant buddings, the old leaf of periphery is removed, removes whole lotus throne leaves after bolting, inhibits side a kind of sedge to grow, most
Plant is set to only have main a kind of sedge to bloom eventually.
(4) pollination is handled
When the main a kind of sedge of plant the flowers are in blossom put 3-4 after, to the mature bud of inflorescence outermost carry out artificial stripping flower bud selfing or
Remove stamen hybridization, it is made normally to set seeds.After 5-7 days, gone after the mature bud of inflorescence outermost can generate the silique of elongation
Fall immature bud on inflorescence.
(5) seed harvest and breaking dormancy
When successfully silique of pollinating starts elongation, blade all on plant is destroyed, only retains silique, to accelerate
The maturation of seed, the prolongation of Hoagland ' s nutrient solution are once a week.After pollination 25 days, silique is removed, by seed
Kind skin removes, and is seeded on culture dish, starts the breeding of next generation.
Preferably, the vernalization treatment time is 15-20 days in step 1).
Preferably, the environment temperature in greenhouse is controlled at 24-26 DEG C.
Compared with prior art, the present invention has the following advantages and beneficial effects:
The present invention comprehensively utilizes multiple technologies means and overcomes vernalization, illumination, temperature according to the habit of Chinese cabbage
Influence of the factors such as degree, plant type, seed dormancy to plant development finally realizes the quick adding generation of Chinese cabbage, pole
Big improves breeding efficiency.In the material for examination, material ' QR2 ' the completion breeding time of strong winter habit only needs 102 days, the weak winter
The material ' Chinese is excellent ' of property then needs the less time, reaches 69 days.Therefore, it will be realized in greenhouse using method provided by the invention
The propagation generation in Chinese cabbage at least a year 3-4 generation.
Detailed description of the invention
Fig. 1 is the young plant after Chinese cabbage transplanting.
Fig. 2 is Chinese cabbage illumination cultivation in the greenhouse.
Fig. 3 is the Chinese cabbage in squaring period.
Fig. 4 is the Chinese cabbage in florescence
Specific embodiment
Below with reference to embodiment, the invention will be further described.
Chinese cabbage material used in the present embodiment has two parts: portion is self-mating system ' QR2 ' (Wuhan City's agriculture of strong winter habit
The breeding of vegetables institute of institute of section), another for weak winter habit kind ' Chinese is excellent ' (breeding of vegetables institute of academy of agricultural sciences of Wuhan City).
1. seed treatment
1.1 germinations: each 20, seed for selecting Chinese cabbage material ' QR2 ' and ' Chinese is excellent ' full seed, respectively
It is put into culture dish, upper one layer of filter paper is padded in the bottom of ware, in addition a little pure water, enables the abundant imbibition of seed, but do not influence it
Respiration is advisable.Culture dish is put into 25 DEG C of incubators, seed germinating is observed.
The test of 1.2 vernalization treatment times: after 2 days, the seed of ' QR2 ' and ' Chinese is excellent ' is all exposed the bud of white, but cotyledon
Not yet it is unfolded, seed is put into refrigerator, adjusting refrigerator temperature is 2 DEG C, and it is 8 days, 15 days and 25 that the processing time, which is respectively set,
It, does not during which need illumination.As a result, it has been found that the vernalization treatment time is longer, material bolting, the time for needs of blooming are shorter.In conjunction with
The time of vernalization treatment in refrigerator, we show that the 15 days time of vernalization treatment can farthest shorten the breeding time of plant
(table 1).
The breeding time of Chinese cabbage under the different vernalization time-triggered protocols of table 1.
2. plant is planted
2.1 sowings: after the completion of vernalization treatment, the seed of ' QR2 ' and ' Chinese is excellent ' is all exposed two because of the reason of low-temperature epitaxy
Piece cotyledon, but be not fully extended.It is (public purchased from the clear biotechnology in Wuhan day that the seed of two materials is seeded into Nutrition Soil respectively
Department) in, each nutritive cube puts a seed, and watering makes its normal growth.Nutritive cube is placed on tissue culture frame, lamp is at
Under the irradiation of pipe.
2.2 exposure experiments to light:
In order to determine the optimum illumination condition that can promote flowering of plant, we carry out test for light quality and illumination length,
The seed of ' QR2 ' and ' Chinese is excellent ' after vernalization 15 days is put into 4 incandescent lamp tubes (power 36W) and 4 full spectrum lamps of LED respectively
Pipe (customizes, and adjusting light quality is full spectrum, spectral band is between 340-800nm, power from Shandong Gui Xiang Photoelectric Co., Ltd.
On tissue culture frame for 36W) composition.Then under two kinds of illumination conditions, light application time is set as 3 gradients, respectively 12 hours,
16 hours and 24 hours, the environment temperature in greenhouse was controlled at 25 DEG C.As a result, it has been found that (table 2), ' QR2 ' and ' Chinese is excellent ' is having the same
Characteristic is experienced in illumination, in the breeding time under the full spectrum fluorescent tube of LED than identical light application time under incandescent lamp breeding time it is short, show
The full spectrum fluorescent tube of LED can advantageously promote Chinese cabbage and bloom.Meanwhile under the conditions of identical light quality, light application time is longer, plants
The breeding time of strain is shorter.Therefore, we determined that plant can maximum journey under the conditions of continuous illumination in 24 hours of LED full spectrum fluorescent tube
Spend to shorten the breeding time of Chinese cabbage.
The breeding time of Chinese cabbage under 2. different light medium of table and illumination condition (from the time spent is seeded into out)
The culture of 2.3 plant: it after germination is completed, puts it on the culturing rack of LED lamp tube composition, carries out 24 hours
Continuous illumination, until seed harvests.During culture, at interval of two day time supplement Hoagland ' s nutrient solution (formula are as follows:
Calcium nitrate 945mg/L, potassium nitrate 607mg/L, ammonium phosphate 115mg/L, magnesium sulfate 493mg/L, iron salt solutions 2.5ml/L are micro
Element 5ml/L, pH=6.0, wherein iron salt solutions: ferrous sulfate heptahydrate 2.78g, disodium ethylene diamine tetraacetate 3.73g, distilled water
500ml adjusts pH=5.5；Liquid microelement: potassium iodide 0.83mg/l, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate
8.6mg/L, sodium molybdate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L), the environment temperature control in greenhouse exists
25℃。
3. buds dwarfing
After plant buddings, the old leaf of periphery is removed, to prevent its excessive growth, while showing that plant has been introduced into life
Generative growth phase.Subsequent statistical result show all plant of material ' QR2 ' and ' Chinese is excellent ' greenhouse can normal bolting,
Wherein ' QR2 ' is 48.5 days from the average time for being seeded into bolting, and ' Chinese is excellent ' is 21.4 from the average time for being seeded into bolting
It.Remove whole lotus throne leaves of plant in the bolting stage, inhibits side a kind of sedge to grow, plant is finally made to only have main a kind of sedge to bloom；When first
Piece the flowers are in blossom put after investigation plant from time spent discovery, ' QR2 ' is seeded into out need 62.1 days, and ' Chinese is excellent ' needs 28.7 days
(table 1, table 2).
4. pollination is handled
When the main a kind of sedge of plant the flowers are in blossom put 3-4 after, artificial stripping flower bud selfing is carried out to the mature bud of inflorescence outermost, makes it
Normally it can set seeds and mark.Retain immature bud on inflorescence simultaneously, to prevent the mature bud of periphery from cannot generate
Seed.After 5-7 days, remove all immature flowers on inflorescence after the mature bud of inflorescence outermost can generate the silique of elongation
Flower bud.
5. seed harvest and breaking dormancy
When successfully silique of pollinating starts elongation, blade all on plant is destroyed, only retains silique, to accelerate
The maturation of seed is simultaneously stopped the supply of Hoagland ' s nutrient solution, supplements a small amount of moisture every 5 days, and plant is made to be in opposite
In the environment of arid.25 days after pollination, silique is removed, the kind skin of seed is removed, and is seeded on culture dish.After 2-3 days, kind
Son is sprouted, and cotyledon starts to grow, is placed into refrigerator and carries out vernalization treatment, starts the breeding of next generation.
In the material for examination, material ' QR2 ' the completion breeding time of strong winter habit only needs 102 days, the material ' Chinese of weak winter habit
It is excellent ' the less time is then needed, reach 69 days.Therefore, Chinese cabbage will be realized using method provided by the invention in greenhouse
The propagation generation in generation at least a year 3-4.
Claims (3)
1. a kind of Chinese cabbage quickly adds the method in generation, which comprises the following steps:
1) seed sprouting and vernalization treatment
It selects full seed to be put into culture dish, water is added to sprout, in the refrigerator after be placed into 2-4 °C after Seed sprouting
Processing 15-25 days, carries out incomplete vernalization treatment；
2) plant is cultivated
The seed of vernalization treatment is transplanted to greenhouse, is seeded into Nutrition Soil, is placed on tissue culture frame, the LED lamp tube of full spectrum
Lower continuous illumination culture in 24 hours, spectral band is between 340-800nm, during culture, supplements at interval of two day time primary
Hoagland ' s nutrient solution；
3) buds dwarfing
After plant buddings, the old leaf of periphery is removed, removes whole lotus throne leaves after bolting, inhibits side a kind of sedge to grow, finally makes
Plant only has main a kind of sedge to bloom；
4) pollination is handled
When the main a kind of sedge of plant the flowers are in blossom put 3-4 after, artificial stripping flower bud selfing is carried out to the mature bud of inflorescence outermost or is removed
Stamen hybridization, makes it normally set seeds, after 5-7 days, removes flower after the mature bud of inflorescence outermost can generate the silique of elongation
Immature bud in sequence；
5) seed harvest and breaking dormancy
When successfully silique of pollinating starts elongation, blade all on plant is destroyed, retains silique, only to accelerate seed
Maturation, be simultaneously stopped the supply of Hoagland ' s nutrient solution, interval keeps the skin wet, after pollination after 25 days, remove silique, will
The kind skin of seed removes, and is seeded on culture dish, starts the breeding of next generation.
2. Chinese cabbage according to claim 1 quickly adds the method in generation, which is characterized in that vernalization treatment in step 1)
Time is 15-20 days.
3. Chinese cabbage according to claim 1 quickly adds the method in generation, which is characterized in that the environment temperature control in greenhouse
System is at 24-26 °C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811553171.1A CN109362505B (en) | 2018-12-18 | 2018-12-18 | Method for rapidly adding generations of non-heading Chinese cabbages |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811553171.1A CN109362505B (en) | 2018-12-18 | 2018-12-18 | Method for rapidly adding generations of non-heading Chinese cabbages |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109362505A true CN109362505A (en) | 2019-02-22 |
| CN109362505B CN109362505B (en) | 2021-06-11 |
Family
ID=65370815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811553171.1A Active CN109362505B (en) | 2018-12-18 | 2018-12-18 | Method for rapidly adding generations of non-heading Chinese cabbages |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109362505B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110663470A (en) * | 2019-10-14 | 2020-01-10 | 甘肃农业大学 | Strong winter cabbage type winter rape generation-adding breeding method |
| CN111990103A (en) * | 2020-08-05 | 2020-11-27 | 江苏省农业科学院 | Method for adjusting flowering synchronization of hybrid seed production of non-heading Chinese cabbages |
| CN113597994A (en) * | 2021-08-19 | 2021-11-05 | 南京新创蔬菜分子育种研究院有限公司 | Vernalization treatment method of white stem vegetables |
| CN114375775A (en) * | 2022-01-13 | 2022-04-22 | 任海龙 | Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1404722A (en) * | 2002-11-05 | 2003-03-26 | 蒋树德 | Non-beading Chinese-cabbage male-sterile-Heibuyu 1 transformation method and its use |
| CN105850717A (en) * | 2016-04-20 | 2016-08-17 | 上海市农业科学院 | Method for acquiring non-heading Chinese cabbage clubroot resistance germplasm resources |
| CN106234207A (en) * | 2016-07-30 | 2016-12-21 | 南京市蔬菜科学研究所 | A kind of method of Chinese cabbage cytoplasmic male sterile line selection-breeding |
| CN107801628A (en) * | 2017-10-27 | 2018-03-16 | 胡月占 | A kind of Chinese cabbage parent greenhouse adds for mating system |
-
2018
- 2018-12-18 CN CN201811553171.1A patent/CN109362505B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1404722A (en) * | 2002-11-05 | 2003-03-26 | 蒋树德 | Non-beading Chinese-cabbage male-sterile-Heibuyu 1 transformation method and its use |
| CN105850717A (en) * | 2016-04-20 | 2016-08-17 | 上海市农业科学院 | Method for acquiring non-heading Chinese cabbage clubroot resistance germplasm resources |
| CN106234207A (en) * | 2016-07-30 | 2016-12-21 | 南京市蔬菜科学研究所 | A kind of method of Chinese cabbage cytoplasmic male sterile line selection-breeding |
| CN107801628A (en) * | 2017-10-27 | 2018-03-16 | 胡月占 | A kind of Chinese cabbage parent greenhouse adds for mating system |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110663470A (en) * | 2019-10-14 | 2020-01-10 | 甘肃农业大学 | Strong winter cabbage type winter rape generation-adding breeding method |
| CN111990103A (en) * | 2020-08-05 | 2020-11-27 | 江苏省农业科学院 | Method for adjusting flowering synchronization of hybrid seed production of non-heading Chinese cabbages |
| CN113597994A (en) * | 2021-08-19 | 2021-11-05 | 南京新创蔬菜分子育种研究院有限公司 | Vernalization treatment method of white stem vegetables |
| CN114375775A (en) * | 2022-01-13 | 2022-04-22 | 任海龙 | Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage |
| CN114375775B (en) * | 2022-01-13 | 2022-11-29 | 任海龙 | Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109362505B (en) | 2021-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109362505A (en) | A kind of Chinese cabbage quickly adds the method in generation | |
| CN101536641B (en) | Guangxi curcuma zedoary flowering phase regulation method | |
| CN106538395A (en) | A kind of method for improving endangered plants Tibet Paeonia ludlowii planting percent and seedling percent | |
| CN101690461B (en) | Method for preparing triploid plants | |
| CN106386488B (en) | It is a kind of to improve big flower pattern pocket orchid seed germination rate and its cultural method | |
| CN109691370A (en) | A kind of radish quickly adds the method in generation | |
| CN107801628B (en) | A kind of Chinese cabbage parent greenhouse adds for mating system | |
| CN106417014B (en) | A kind of big flower pattern pocket orchid breeding and cultural method | |
| CN101536663B (en) | Guangxi zedoary hydroponic blooming method | |
| CN103609433B (en) | Breeding method for bolting resistant radish selfing line | |
| CN101703003B (en) | Method for quickly propagating grassiness | |
| CN106665304B (en) | Method for rapid seedling formation of hybrid seeds of jujube trees based on soilless culture | |
| CN108703064A (en) | A kind of Small-sized watermelon cross breeding method | |
| CN110915656B (en) | Female-line cucumber double haploid regeneration plant and preparation method and application thereof | |
| CN115553211B (en) | Breeding method for quickly obtaining pure line by wheat hybridization | |
| CN109924076B (en) | Breeding method for shortening dragon fruit crossbreeding period | |
| CN106961992A (en) | A kind of cassava true seed seeding growing seedlings method | |
| CN103125391B (en) | Local multi-generation breeding method for soybean | |
| CN111972074B (en) | Seed treatment method for early-maturing sweet cherry variety and method for sowing seedlings in current year | |
| CN107950399B (en) | Method for improving utilization and screening efficiency of potato seedling seeds | |
| CN101889548A (en) | Cabbage haploid breeding method | |
| CN101263784B (en) | Rapeseed cultivar stem segment tissue culture fast breeding male sterile lines seed making method | |
| CN104938325A (en) | Strawberry hybridization and seed seedling method | |
| CN110896837B (en) | High-quality and high-yield planting method for andrographis paniculata | |
| CN107318556A (en) | Ulmus elongata fruit in-vitro breeding and its seeding growing seedlings method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |