CN114375775A - Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage - Google Patents
Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage Download PDFInfo
- Publication number
- CN114375775A CN114375775A CN202210036083.4A CN202210036083A CN114375775A CN 114375775 A CN114375775 A CN 114375775A CN 202210036083 A CN202210036083 A CN 202210036083A CN 114375775 A CN114375775 A CN 114375775A
- Authority
- CN
- China
- Prior art keywords
- flowering
- cabbage
- flowering cabbage
- purple
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a breeding method for quickly transferring a bolting gene to a flowering cabbage, which comprises the following steps: (1) after sowing the flowering cabbage and the purple flowering cabbage respectively, carrying out light-dark alternate culture on the flowering cabbage at the constant temperature of 25 ℃; carrying out light-dark alternate culture on the brassica oleracea stems at the temperature change condition of 25 ℃/10 ℃; respectively applying water-soluble compound fertilizers when the culture lasts for 20-30 days; (2) when the flowering is performed by bolting the flowering cabbage heart and the purple flowering cabbage, hybridizing by taking the purple flowering cabbage as a female parent and the flowering cabbage as a male parent to obtain F1 hybrid seeds; (3) performing bark cutting treatment on the F1 hybrid seeds, sowing, culturing according to the cabbage cultivation mode in the step (1), and performing backcross with the cabbage when the F1 plant blooms to obtain BC1 seeds; (4) and (4) repeating the step (3) to carry out continuous multiple-generation backcross. The invention can be bred for one generation in 1.5 months, greatly shortens breeding time, saves a large amount of labor and land cost, and realizes annual breeding of the flowering cabbage under the artificial controllable environment.
Description
Technical Field
The invention belongs to the technical field of breeding, and particularly relates to a breeding method for quickly transferring a brassica oleracea bolting gene to a flowering cabbage.
Background
Flowering Chinese cabbage, also called as flowering Chinese cabbage, is a biennial herb in brassica species of brassica in cruciferae, takes flowering Chinese cabbage as an edible product, is mainly distributed in south China, has no strict requirement on low temperature for bolting and flowering, and can be produced in the south China all the year round. The brassica chinensis bolts are special vegetables in China, originate from the middle of the Yangtze river basin, the places of Changsha in Hunan, Huntan, Wuhan in Hubei, Sichuan Chengdu and the like, have long cultivation history, and require a certain low temperature for bolting and flowering. Interspecific hybridization is an important means for widening genetic germplasm resources and creating new species and new materials. Because the flowering requirements of the flowering cabbage heart and the purple flowering cabbage are different, flowering asynchronism is caused, the flowering cabbage is difficult to be improved by using the excellent genes of the purple flowering cabbage in breeding, the breeding process of obtaining a new variety through crossbreeding generally needs 6-8 generations in production, only 2 generations can be bred in each year under field conditions, and labor and time are consumed.
Disclosure of Invention
Aiming at the prior art, the invention provides a breeding method for quickly transferring the gene of the brassica oleracea to the flowering cabbage, and aims to solve the problems that the flowering cabbage is not encountered with the flowering cabbage, the flowering cabbage is difficult to be improved by using the excellent gene of the brassica oleracea, the breeding time is long, and the like.
In order to achieve the purpose, the invention adopts the technical scheme that: provides a breeding method for quickly transferring a bolting gene to a flowering cabbage, which comprises the following steps:
(1) respectively sowing the flowering cabbage and the purple flowering cabbage, and performing light-dark alternate culture on the flowering cabbage at the constant temperature of 25 ℃, wherein the light-dark alternate period is 16h/8h, and the illumination intensity is 250umol/m2s, the illumination spectrum is white: red-3: 1; culturing the purple flowering cabbage under variable temperature conditions with light and dark alternation cycle at 16h, 25 deg.C/8 h, 10 deg.C and illumination intensity of 250umol/m2s; when the flowering cabbage and the purple flowering cabbage are cultured for 20-30 days, respectively applying a water-soluble compound fertilizer;
(2) when flowering in the bolting of flowering cores and the brassica oleracea, hybridizing by taking the brassica oleracea as a female parent and the flowering cores as a male parent, removing growing points at the top ends of the hybridized brassica oleracea flowering stems, retaining pods at the middle and lower parts of the flowering stems, removing side stems, simultaneously applying a water-soluble compound fertilizer, reducing the water application amount after seed pods change color, and then obtaining F1 hybrid seeds;
(3) performing bark cutting treatment on the F1 hybrid seeds, sowing, culturing according to the cabbage cultivation mode in the step (1), and performing backcross with the cabbage when the F1 plant blooms to obtain BC1 seeds;
(4) and (4) repeating the step (3) to carry out continuous multiple-generation backcross.
Under the specific light intensity and spectrum conditions, the flowering cabbage and the purple flowering cabbage can bolt and flower in about 28 days, and the artificial hybridization can be conveniently carried out. Proper drought is carried out after the seed pod is discolored, which is favorable for rapid maturity of seeds. According to the invention, the excellent genes of the brassica oleracea are transferred into the flowering cabbage through artificial hybridization, so that the flowering cabbage with the flavor and color of the brassica oleracea is cultured, and the nutritional value of the flowering cabbage is improved. And backcrossing for many times to obtain the strain with homozygous genotype and stable character.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the water-soluble compound fertilizer comprises 20-30 parts of monoammonium phosphate, 30-40 parts of potassium nitrate, 30-40 parts of ammonium nitrate, 2-3 parts of brassin and 2-3 parts of sugar alcohol molybdenum.
The water-soluble compound fertilizer can be dissolved more completely in water, and the crop absorption and utilization speed is higher and more complete. The brassin can effectively increase the chlorophyll content, improve the photosynthesis efficiency, regulate the nutrient distribution, promote the transportation of carbohydrates of stems and leaves to granules, promote the roots and the seedlings, and protect the flowers and the fruits; the cold resistance, drought resistance, salt and alkali resistance and other stress resistances of crops are improved, and the occurrence of diseases is obviously reduced; and can remarkably relieve the occurrence of phytotoxicity, quickly recover the growth of crops and eliminate disease spots. The sugar alcohol molybdenum can promote the chlorophyll formation of crops, and has the advantages of early flowering, early maturity and full seeds. By applying the water-soluble compound fertilizer to the action, the nutrition required by crops can be comprehensively supplemented, the full absorption and utilization of the fertilizer are ensured, the growth of the flowering cabbage, the brassica oleracea and the filial generation thereof is effectively promoted, and the generation of full seeds is promoted.
Further, the hybridization mode in the step (2) is artificial hybridization.
Further, the step (3) of peeling comprises the steps of peeling, and soaking in warm water at the temperature of 25-35 ℃ for 24 hours.
Further, the multiple backcross in the step (5) is 4-5 backcross generations.
The invention also provides a breeding culture shelf suitable for the breeding method, which comprises a multilayer support, wherein the support comprises a storage plate at the top and supporting legs arranged at the bottom of the storage plate, a fixing groove matched with the lower ends of the supporting legs is formed in the storage plate, an LED light supplement lamp and a water dropping pipe are arranged at the bottom of the storage plate, and a water dropping opening is formed in the water dropping pipe.
When the support is used, the support legs of the upper layer support can be placed in the fixing grooves, and therefore the stability of the support is guaranteed. The multilayer detachable support is arranged, so that the carrying and the installation are convenient, and the space can be saved. The object placing plate is used for placing the incubator, the LED light supplementing lamp is used for supplementing illumination for action cultivation, and the water dripping pipe is used for watering, supplementing nutrient substances and the like for the action cultivation.
Furthermore, the support is square, the length, the width and the height of the support are 1800mm, 300mm and 600mm in sequence, and the support legs are arranged at four corners of the support.
The bracket is arranged into a cuboid shape, and can be used for placing two rows of crops, so that the manual hybridization operation is convenient. The supporting legs set up in the support four corners, and is more stable.
Further, the support is provided with three layers.
The support is arranged into three layers, the total height of the support is 1.8m, and the height of the support is convenient for manual hybridization operation.
The invention has the beneficial effects that:
1. according to the invention, the flowering cabbage and the purple flowering cabbage are cultivated under the specific light intensity and spectrum conditions, so that the flowering cabbage can be bolting and flowering in about 28 days, and further the two can be hybridized. According to the invention, the growth of the flowering cabbage, the purple flowering cabbage and the filial generation thereof is promoted by applying the specific water-soluble compound fertilizer, and the generation of full seeds is promoted.
2. According to the invention, the dormancy of seeds is broken through the peeling treatment, so that the hybrid seeds of the flowering cabbage and the purple flowering Chinese cabbage can obtain the strains with homozygous genotypes and stable characters for about 1 year, the breeding time is greatly shortened, and the breeding efficiency is greatly improved.
3. When the isolated population is purified, the field breeding is not needed, a large amount of labor and land cost is saved, and the annual breeding of the flowering cabbage under the artificial controllable environment is realized.
4. The method of the invention is effective for early-maturing, medium-maturing and late-maturing varieties of the flowering cabbage and has wide application range.
Detailed Description
The following examples are provided to illustrate specific embodiments of the present invention.
Example 1
A breeding method for quickly transferring a brassica oleracea gene to a flowering cabbage comprises the following steps:
(1) respectively sowing the flowering cabbage and the purple flowering cabbage, and performing light-dark alternate culture on the flowering cabbage at the constant temperature of 25 ℃, wherein the light-dark alternate period is 16h/8h, and the illumination intensity is 250umol/m2s, the illumination spectrum is white: red-3: 1; culturing the purple flowering cabbage under variable temperature conditions with light and dark alternation cycle at 16h, 25 deg.C/8 h, 10 deg.C and illumination intensity of 250umol/m2s; when the flowering cabbage and the purple flowering cabbage are cultured for 20 days, respectively applying a water-soluble compound fertilizer;
(2) when flowering in the bolting of flowering cabbage and the purple flowering cabbage, manually hybridizing the purple flowering cabbage as a female parent and the flowering cabbage as a male parent, removing growing points at the top ends of the hybridized purple flowering cabbage, retaining pods at the middle and lower parts of the flowering cabbage, removing side flowering cabbage, simultaneously applying a water-soluble compound fertilizer, reducing the water application amount of 2/3 after the seed pods change color, and obtaining F1 hybrid seeds;
(3) cutting seed coat of F1 hybrid seed, soaking in 30 deg.C warm water for 24 hr, sowing, performing light-dark alternate culture at 25 deg.C under constant temperature with light-dark alternate period of 16 hr/8 hr and illumination intensity of 250umol/m2s, the illumination spectrum is white: red-3: 1, applying a water-soluble compound fertilizer when the F1 plants grow for 25 days, and then backcrossing with the flowering cabbage when the F1 plants bloom to obtain BC1 seeds;
(4) and (4) repeating the step (3) to carry out continuous 3-generation backcross.
The water-soluble compound fertilizer is prepared by mixing 25 parts of monoammonium phosphate, 35 parts of potassium nitrate, 35 parts of ammonium nitrate, 3 parts of brassin and 2 parts of sugar alcohol molybdenum.
Example 2
A breeding method for quickly transferring a brassica oleracea gene to a flowering cabbage comprises the following steps:
(1) cabbage heart and laverRespectively sowing the young shoots, and culturing the flowering Chinese cabbage under the constant temperature of 25 ℃ in light-dark alternating mode with the light-dark alternating period of 16h/8h and the illumination intensity of 250umol/m2s, the illumination spectrum is white: red-3: 1; culturing the purple flowering cabbage under the condition of controlling the temperature and carrying out light-dark alternate culture with the light-dark alternate period and the temperature of 16h, 25 ℃/8h and 10 ℃ and the illumination intensity of 250umol/m2s; when the flowering cabbage and the purple flowering cabbage are cultured for 25 days, respectively applying a water-soluble compound fertilizer;
(2) when flowering in the bolting of flowering cabbage and the purple flowering cabbage, manually hybridizing the purple flowering cabbage serving as a female parent and the flowering cabbage serving as a male parent, removing growing points at the top ends of the hybridized purple flowering cabbage, retaining pods at the middle and lower parts of the flowering cabbage, removing side flowering cabbage, simultaneously applying a water-soluble compound fertilizer, reducing the water application amount of 2/3 after the seed pods change color, and then obtaining F1 hybrid seeds;
(3) grinding seed coat of F1 hybrid seed, soaking in 25 deg.C warm water for 24 hr, sowing, performing light-dark alternate culture at 25 deg.C under constant temperature condition with light-dark alternate period of 16 hr/8 hr and illumination intensity of 250umol/m2s, the illumination spectrum is white: red-3: 1, applying a water-soluble compound fertilizer when F1 plants grow for 20 days, and then backcrossing with flowering cabbage when F1 plants bloom to obtain BC1 seeds;
(4) and (4) repeating the step (3) to carry out continuous backcross for 4 generations.
The water-soluble compound fertilizer is prepared by mixing 20 parts of monoammonium phosphate, 30 parts of potassium nitrate, 30 parts of ammonium nitrate, 2 parts of brassin and 3 parts of sugar alcohol molybdenum.
Example 3
A breeding method for quickly transferring a brassica oleracea gene to a flowering cabbage comprises the following steps:
(1) respectively sowing the flowering cabbage and the purple flowering cabbage, and performing light-dark alternate culture on the flowering cabbage at the constant temperature of 25 ℃, wherein the light-dark alternate period is 16h/8h, and the illumination intensity is 250umol/m2s, the illumination spectrum is white: red-3: 1; culturing the purple flowering cabbage under the condition of controlling the temperature and carrying out light-dark alternate culture with the light-dark alternate period and the temperature of 16h, 25 ℃/8h and 10 ℃ and the illumination intensity of 250umol/m2s; when the flowering cabbage and the purple flowering cabbage are cultured for 30 days, respectively applying a water-soluble compound fertilizer;
(2) when flowering in the bolting of flowering cabbage and the purple flowering cabbage, manually hybridizing the purple flowering cabbage serving as a female parent and the flowering cabbage serving as a male parent, removing growing points at the top ends of the hybridized purple flowering cabbage, retaining pods at the middle and lower parts of the flowering cabbage, removing side flowering cabbage, simultaneously applying a water-soluble compound fertilizer, reducing the water application amount of 2/3 after the seed pods change color, and then obtaining F1 hybrid seeds;
(3) grinding seed coat of F1 hybrid seed, soaking in 35 deg.C warm water for 24 hr, sowing, performing light-dark alternate culture at 25 deg.C under constant temperature with light-dark alternate period of 16 hr/8 hr and illumination intensity of 250umol/m2s, the illumination spectrum is white: adding water-soluble compound fertilizer when the F1 plants grow for 30 days; then backcrossing with flowering cabbage when F1 plants bloom to obtain BC1 seeds;
(4) and (4) repeating the step (3) to carry out continuous 5-generation backcross.
The water-soluble compound fertilizer is prepared by mixing 30 parts of monoammonium phosphate, 40 parts of potassium nitrate, 40 parts of ammonium nitrate, 3 parts of brassin and 3 parts of sugar alcohol molybdenum.
The invention also provides a breeding culture shelf suitable for the breeding method, which comprises a plurality of layers of square supports, wherein the supports are 1800mm, 300mm and 600mm in length, width and height, the supports comprise a top object placing plate and supporting legs arranged at four corners of the bottom of the object placing plate, fixing grooves matched with the lower ends of the supporting legs are arranged on the object placing plate, LED light supplementing lamps and water dripping pipes are arranged at the bottom of the object placing plate, and water dripping ports are formed in the water dripping pipes. Preferably, the stent is provided in three layers.
Comparative example 1
The light-dark alternation period in example 1 was changed to 12h/12h, and the rest was the same as in example 1.
Comparative example 2
The light intensity in example 1 was changed to 200umol/m2s, and the rest was the same as in example 1.
Comparative example 3
The seed coat cutting in example 1 was changed to direct seeding, and the rest was the same as in example 1.
Comparative example 4
The water-soluble compound fertilizer in the example 1 is changed into 25 parts of monoammonium phosphate, 35 parts of potassium nitrate and 35 parts of ammonium nitrate, and the rest is the same as the example 1.
In the following, statistics are given on the bolting time, thousand seed weight, seed germination rate and silique effective grain number of the flowering Chinese cabbage male parent, the purple flowering Chinese cabbage female parent and the filial generation in the planting method of the embodiment and the comparative example of the present invention, and the results are shown in table 1. It can be seen that the flowering time of the flowering cabbage heart and the flowering time of the purple flowering cabbage can be the same by controlling the illumination time and the illumination intensity, and the hybridization is convenient. By controlling the illumination time and intensity and the type of the water-soluble compound fertilizer, the development atrophy of fruit pods and the bending burst of the fruit pods can be effectively avoided, and meanwhile, the growth of the root system of the flowering cabbage can be promoted, the flowering cabbage pollen quality can be improved, and further the seed quality and quality of the flowering cabbage can be improved.
TABLE 1
While the present invention has been described in detail with reference to the embodiments, it should not be construed as limited to the scope of the patent. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.
Claims (8)
1. A breeding method for quickly transferring a brassica oleracea gene to a flowering cabbage is characterized by comprising the following steps:
(1) respectively sowing the flowering cabbage and the purple flowering cabbage, and performing light-dark alternate culture on the flowering cabbage at the constant temperature of 25 ℃, wherein the light-dark alternate period is 16h/8h, and the illumination intensity is 250umol/m2s, the illumination spectrum is white: red-3: 1; culturing the purple flowering cabbage under variable temperature conditions with light and dark alternation cycle at 16h, 25 deg.C/8 h, 10 deg.C and illumination intensity of 250umol/m2s; when the flowering cabbage and the purple flowering cabbage are cultured for 20-30 days, respectively applying a water-soluble compound fertilizer;
(2) when flowering in the bolting of flowering cores and the brassica oleracea, hybridizing by taking the brassica oleracea as a female parent and the flowering cores as a male parent, removing growing points at the top ends of the hybridized brassica oleracea flowering stems, retaining pods at the middle and lower parts of the flowering stems, removing side stems, simultaneously applying a water-soluble compound fertilizer, reducing the water application amount after seed pods change color, and then obtaining F1 hybrid seeds;
(3) performing bark cutting treatment on the F1 hybrid seeds, sowing, culturing according to the cabbage cultivation mode in the step (1), and performing backcross with the cabbage when the F1 plant blooms to obtain BC1 seeds;
(4) and (4) repeating the step (3) to carry out continuous multiple-generation backcross.
2. A breeding method according to claim 1, characterized in that: the water-soluble compound fertilizer in the steps (1) and (2) is formed by mixing 20-30 parts of monoammonium phosphate, 30-40 parts of potassium nitrate, 30-40 parts of ammonium nitrate, 2-3 parts of brassin and 2-3 parts of sugar alcohol molybdenum.
3. A breeding method according to claim 1, characterized in that: the hybridization mode in the step (2) is artificial hybridization.
4. A breeding method according to claim 1, characterized in that: the step (3) of peeling treatment is to cut the seed coat and then soak the seed coat in warm water at the temperature of 25-35 ℃ for 24 hours.
5. A breeding method according to claim 1, characterized in that: in the step (4), the multiple backcross is 4-5 backcross.
6. A breeding culture rack suitable for use in the breeding method according to any one of claims 1 to 5, characterized in that: including the multilayer support, the support includes that the thing board is put at the top and sets up in the supporting legs of putting thing board bottom, put be provided with on the thing board with the fixed slot that the supporting legs lower extreme matches, the bottom of putting the thing board is provided with LED light filling lamp and drip, be provided with the mouth that drips on the drip.
7. A breeding culture rack according to claim 6, characterized in that: the support is square, and its length, width and height are 1800mm, 300mm, 600mm in proper order, the supporting legs set up in the four corners of support.
8. A breeding culture rack according to claim 7, characterized in that: the support is provided with three layers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210036083.4A CN114375775B (en) | 2022-01-13 | 2022-01-13 | Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210036083.4A CN114375775B (en) | 2022-01-13 | 2022-01-13 | Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114375775A true CN114375775A (en) | 2022-04-22 |
| CN114375775B CN114375775B (en) | 2022-11-29 |
Family
ID=81202763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210036083.4A Active CN114375775B (en) | 2022-01-13 | 2022-01-13 | Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114375775B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777528A (en) * | 2023-02-09 | 2023-03-14 | 广州市农业科学研究院 | Culture method for improving microspore embryo emergence rate of hybrid seeds of flowering cabbage and brassica oleracea |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358630A (en) * | 2016-08-19 | 2017-02-01 | 江西省农业科学院作物研究所 | Brassica napus annual multi-generation generation adding method |
| CN107750889A (en) * | 2017-10-20 | 2018-03-06 | 成都先先先生物科技有限公司 | A kind of a kind of sedge dish nutrient solution for not producing pollution peculiar smell and preparation method thereof |
| CN109362505A (en) * | 2018-12-18 | 2019-02-22 | 武汉市农业科学院 | A kind of Chinese cabbage quickly adds the method in generation |
| CN111990103A (en) * | 2020-08-05 | 2020-11-27 | 江苏省农业科学院 | Method for adjusting flowering synchronization of hybrid seed production of non-heading Chinese cabbages |
| CN113040048A (en) * | 2021-03-18 | 2021-06-29 | 河南省农业科学院经济作物研究所 | Method for rapidly identifying purity of rape hybrid seeds indoors |
| CN113598043A (en) * | 2021-08-05 | 2021-11-05 | 四川省农业科学院经济作物育种栽培研究所 | Indoor multi-generation peanut hybridization method |
-
2022
- 2022-01-13 CN CN202210036083.4A patent/CN114375775B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358630A (en) * | 2016-08-19 | 2017-02-01 | 江西省农业科学院作物研究所 | Brassica napus annual multi-generation generation adding method |
| CN107750889A (en) * | 2017-10-20 | 2018-03-06 | 成都先先先生物科技有限公司 | A kind of a kind of sedge dish nutrient solution for not producing pollution peculiar smell and preparation method thereof |
| CN109362505A (en) * | 2018-12-18 | 2019-02-22 | 武汉市农业科学院 | A kind of Chinese cabbage quickly adds the method in generation |
| CN111990103A (en) * | 2020-08-05 | 2020-11-27 | 江苏省农业科学院 | Method for adjusting flowering synchronization of hybrid seed production of non-heading Chinese cabbages |
| CN113040048A (en) * | 2021-03-18 | 2021-06-29 | 河南省农业科学院经济作物研究所 | Method for rapidly identifying purity of rape hybrid seeds indoors |
| CN113598043A (en) * | 2021-08-05 | 2021-11-05 | 四川省农业科学院经济作物育种栽培研究所 | Indoor multi-generation peanut hybridization method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777528A (en) * | 2023-02-09 | 2023-03-14 | 广州市农业科学研究院 | Culture method for improving microspore embryo emergence rate of hybrid seeds of flowering cabbage and brassica oleracea |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114375775B (en) | 2022-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103733859B (en) | A kind of cassava blooms control technique | |
| CN102893851B (en) | Butterfly orchid breeding method | |
| CN102487816B (en) | Method for producing wheat double haploid by wheat-and-maize distant hybridization | |
| CN102090316A (en) | Method for producing rice hybrid Guangliangyou 206 | |
| CN101919351A (en) | Production method of hybrid rice | |
| CN110169346B (en) | Cherry tomato soilless culture method suitable for tropical area | |
| CN109287486B (en) | Paphiopedilum seed germination rate improving method and paphiopedilum cultivation method | |
| CN103858754A (en) | Breeding method of novel high-yield out-of-season garlic | |
| CN110235776A (en) | A kind of tomato heliogreenhouse producing method for seed | |
| CN106417014B (en) | A kind of big flower pattern pocket orchid breeding and cultural method | |
| CN114375775B (en) | Breeding method for quickly transferring purple flowering cabbage gene to flowering cabbage | |
| CN101263783B (en) | Method for using herbicide barban to fast breed aloe all-male plant strain | |
| CN105940901A (en) | Method for postponing bolting of Wuta-tsai | |
| CN105359972A (en) | Wheat mature embryo callus induction culture and plant regeneration system construction method | |
| Saravia-Castillo et al. | Auxins and Cytokinins elicit a differentiated response in the formation of shoots and roots in Cattleya maxima Lindl and Phalaenopsis amabilis (L) Blume | |
| CN109197204B (en) | Method for cultivating new variety of pitaya with high cold resistance | |
| WO2019048382A1 (en) | Method for production of sugarcane seedlings | |
| CN103461097A (en) | Combined seed production method for three-line hybrid oilseed rape | |
| KR20140057917A (en) | Cultivation method of lily bulbs | |
| CN107439220B (en) | High-yield cultivation method for asparagus | |
| Della Rahayu et al. | Asymbiotic seed germination and plantlet development of Dendrobium spectabile (Blume) Miq. | |
| CN117546738B (en) | Efficient seed production method for tomato hybrid seeds | |
| Rajamani et al. | Glory lily (Gloriosa superba): A phytochemical crop of commercial significance | |
| CN210470598U (en) | Tomato soilless culture device | |
| CN110915584B (en) | Method for rapidly propagating lotus in multiple stubbles in one year |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |